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CN222250743U - Modular Space Microbial Experimental Payload - Google Patents

Modular Space Microbial Experimental Payload Download PDF

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Publication number
CN222250743U
CN222250743U CN202420823219.0U CN202420823219U CN222250743U CN 222250743 U CN222250743 U CN 222250743U CN 202420823219 U CN202420823219 U CN 202420823219U CN 222250743 U CN222250743 U CN 222250743U
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China
Prior art keywords
test
culture
microorganism
cavity
frame
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CN202420823219.0U
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Chinese (zh)
Inventor
张秦
印红
张文德
袁俊霞
范作伟
金鹏飞
宋研
党磊
童曈
马玲玲
曹茜
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Space Shenzhou Biology & Technology Group Co ltd
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Space Shenzhou Biology & Technology Group Co ltd
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Priority to CN202420823219.0U priority Critical patent/CN222250743U/en
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Abstract

The utility model relates to the technical field of space tests and provides a modularized space microorganism test load which comprises a shell, a plurality of test devices and a controller, wherein a plurality of observation holes are formed in the front side of the shell, the test devices are distributed in the shell in an array mode and correspond to the observation holes, the test devices are used for in-situ tests of microorganisms in an on-orbit mode, and the controller is arranged in the shell and electrically connected with the test devices and is used for independently controlling the microorganism test process of each test device. The utility model can realize the mutually independent control among a plurality of test devices and simultaneously develop a plurality of same or different types of microorganism tests according to the requirements, thereby effectively improving the microorganism test efficiency and the microorganism test flux.

Description

Modularized space microorganism test load
Technical Field
The utility model relates to the technical field of space tests, in particular to a modularized space microorganism test load.
Background
In the related art, microorganisms have the advantages of simple structure, short growth cycle, rapid propagation, convenient carrying and the like, and are used as biological models for research on life phenomena in space environment, detection of life outside the ground, research on planetary protection tasks taking the microorganisms as important attention objects, and the like.
However, due to the specificity of the space environment, the existing on-orbit microorganism culture test device has small test flux and low integration level, and can only carry out one microorganism test at a time, so that the on-orbit microorganism test efficiency is low, the parallelism among multiple tests is poor, and high-quality research results cannot be obtained.
Disclosure of utility model
The utility model provides a modularized space microorganism test load, which is used for solving the defects of small test flux, low integration level, single test type, low efficiency and the like of a microorganism test device in the related technology, realizing the mutually independent control of a plurality of test devices, and simultaneously carrying out a plurality of microorganism tests of the same or different types according to requirements, thereby effectively improving the microorganism test efficiency and flux.
The utility model provides a modularized space microorganism test load, which comprises the following components:
A housing, a plurality of observation holes being provided on a front side of the housing;
The test devices are distributed in the shell in an array manner and correspond to the observation holes, and the test devices are used for in-situ tests of microorganisms;
And the controller is arranged on the shell, is electrically connected with the test device and is used for independently controlling the microorganism test process of each test device.
According to the utility model there is provided a modular spatial microbiological test load, the housing comprising:
The front side of the first frame is provided with a plurality of first observation holes which are in one-to-one correspondence with the test devices, and the first frame is detachably connected with the test devices;
the front cover plate is detachably connected to the front side of the first frame and is provided with a plurality of second observation holes which are in one-to-one correspondence with the first observation holes;
and a back cover plate detachably connected to the back side of the first frame.
According to the modularized space microorganism test load provided by the utility model, a first sealing ring is respectively arranged at the joint of the first frame and the front cover plate and at the joint of the first frame and the rear cover plate;
and a first transparent observation plate is arranged between the front cover plate and the first frame.
According to the utility model there is provided a modular spatial microbiological test load, the housing comprising:
The front side of the second frame is provided with a plurality of mounting openings in parallel;
The module cavity shells are arranged in the second frame and detachably mounted at the mounting ports in a one-to-one correspondence mode, a plurality of test devices are arranged in the module cavity shells, and observation holes corresponding to the test devices in a one-to-one correspondence mode are formed in the front sides of the module cavity shells.
According to the utility model, there is provided a modular space microbiological test load, the modular chamber housing comprising:
The front side of the cavity shell is provided with a plurality of third observation holes which are in one-to-one correspondence with the test devices;
the front side plate is detachably connected to the front side of the cavity shell, and is provided with a plurality of fourth observation holes which are in one-to-one correspondence with the third observation holes;
and the rear side plate is detachably connected to the rear side of the cavity shell.
According to the modularized space microorganism test load provided by the utility model, the connection part of the cavity shell and the front side plate and the connection part of the cavity shell and the rear side plate are respectively provided with a second sealing ring;
and a second transparent observation plate is arranged between the front side plate and the cavity shell.
According to the modularized space microorganism test load provided by the utility model, the bottoms of two opposite sides of the shell are respectively provided with the mounting bases, and the mounting bases are used for being mounted on a spacecraft;
and at least one mounting base is provided with a socket, and the controller is arranged in the socket.
According to the utility model, a modular spatial microorganism test load is provided, and the test device comprises:
A culture assembly comprising a sealed culture pond;
The gas-liquid storage assembly comprises a storage pool and a turnover membrane, wherein the storage pool is connected with the culture pool, and a first accommodating cavity is formed between the storage pool and the culture pool;
the transmission assembly is arranged in the first accommodating cavity and comprises a liquid inlet pipeline and a gas outlet pipeline, the culture pond is connected with the liquid storage cavity through the liquid inlet pipeline, and the culture pond is connected with the gas storage cavity through the gas outlet pipeline.
According to the modularized space microorganism test load provided by the utility model, the turnover film is provided with the convex part which is convex towards the direction of the gas storage cavity.
According to the utility model there is provided a modular spatial microbiological test load, said culture assembly further comprising:
the heating film is coated on the outer wall of the culture pond;
the heat insulation blocks are arranged on the outer side of the heating film in a surrounding mode at intervals;
The temperature sensor is arranged in the culture pond;
The electric connector is arranged in the first accommodating cavity and is respectively connected with the controller, the liquid inlet pipeline, the heating film and the temperature sensor.
According to the modularized space microorganism test load, a plurality of small-sized and independent test devices are integrated under the effective space and weight constraint conditions, so that the independent control of the test devices can be realized, and a plurality of same or different microorganism tests can be carried out simultaneously according to requirements, so that the microorganism test efficiency and flux are effectively improved.
Drawings
In order to more clearly illustrate the utility model or the technical solutions in the related art, the drawings that are required to be used in the description of the embodiments or the related art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the utility model, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a test device according to the present utility model;
FIG. 2 is a schematic diagram of a second embodiment of the test apparatus according to the present utility model;
FIG. 3 is a schematic exploded view of the structure of the test device provided by the present utility model;
FIG. 4 is a schematic diagram of the structure of the culture pond provided by the utility model;
FIG. 5 is a schematic flow chart of a control method of the test device provided by the utility model;
FIG. 6 is a schematic illustration of one of the structures of a modular spatial microbiological test load provided by the present utility model;
FIG. 7 is an exploded schematic view of a modular spatial microbiological test load provided by the present utility model;
FIG. 8 is a second schematic diagram of a modular spatial microbiological test load provided by the present utility model;
FIG. 9 is a schematic view of a second frame provided by the present utility model;
FIG. 10 is a schematic view of the structure of a modular housing provided by the present utility model;
fig. 11 is an exploded view of a modular housing provided by the present utility model.
Reference numerals:
1, a culture pond, 101, 102, 103, a second accommodating cavity and a first flanging;
104, a first support column, 1041, a connecting hole, 105, a second support column;
1051, a liquid inlet channel, 106, an air outlet hole and 107, a first connecting pipe;
108, a second connecting pipe 109, a connecting column;
2, a storage pool, 201, a liquid storage cavity and 202, a third connecting pipe;
203, a fourth connecting pipe, 204, a pool body, 205, a cover body and 206, a third connecting part;
3, turning over the membrane 301;
4, a first accommodating cavity, 5, a heating film, 6, a heat insulation block and 7, an electric connector;
8, a sample bracket, 801, an overflow gap, 9, a compression frame and 10, a window;
11, pump, 12, heat insulation buffer pad, 121, fixing ring, 122, first connecting part;
13, a wire bundle seat, 131, a first wire bundle hole and 132, a second wire bundle hole;
14, connecting frame, 141, second connecting part, 142, weight reducing hole;
15, a controller 161, a first frame 162, a front cover plate 163 and a rear cover plate;
164, 165, 166, a first sealing ring;
167 a first transparent viewing panel 168 a first stiffener;
171, 172, 173, mounting port, 174, cavity shell;
175 a front side plate, 176 a rear side plate, 177 a third viewing aperture;
178, a fourth observation hole, 179, a second sealing ring and 180, a second transparent observation plate;
181 of second reinforcing ribs, 19 of mounting base and 20 of socket.
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present utility model more apparent, the technical solutions of the present utility model will be clearly and completely described below with reference to the accompanying drawings, and it is apparent that the described embodiments are some embodiments of the present utility model, not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.
In the description of the embodiments of the present utility model, it should be noted that the terms "center", "longitudinal", "lateral", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, are merely for convenience in describing the embodiments of the present utility model and simplifying the description, and do not indicate or imply that the apparatus or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the embodiments of the present utility model. Furthermore, the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In describing embodiments of the present utility model, it should be noted that, unless explicitly stated or limited otherwise, the terms "connected," "connected," and "coupled" should be construed broadly, and may be, for example, fixedly connected, detachably connected, integrally connected, mechanically connected, electrically connected, directly connected, or indirectly connected via an intermediate medium. The specific meaning of the above terms in embodiments of the present utility model will be understood in detail by those of ordinary skill in the art.
In embodiments of the utility model, unless expressly specified and limited otherwise, a first feature "up" or "down" on a second feature may be that the first and second features are in direct contact, or that the first and second features are in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.
In the description of the present specification, a description referring to terms "one embodiment," "some embodiments," "examples," "specific examples," or "some examples," etc., means that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the embodiments of the present utility model. In this specification, schematic representations of the above terms are not necessarily directed to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples. Furthermore, the different embodiments or examples described in this specification and the features of the different embodiments or examples may be combined and combined by those skilled in the art without contradiction.
The in-orbit miniaturized integrated microbiological test device and the modularized space microbiological test load according to the present utility model are described below with reference to fig. 1 to 11.
According to the embodiment of the first aspect of the utility model, referring to fig. 1-4, the on-orbit small-sized integrated microorganism test device provided by the utility model fully considers special challenges in space environment, such as liquid phenomenon change under microgravity condition, material transmission difficulty, sealing safety requirement, volume and the like, and provides corresponding solutions.
The on-orbit small-sized integrated microorganism test device provided by the embodiment of the utility model mainly comprises three parts, namely a culture assembly, a gas-liquid storage assembly and a transmission assembly.
Wherein, the culture assembly comprises a sealed culture pond 1, and the culture pond 1 is used for a culture test of microorganisms. By adopting the sealed culture pond 1 design, the microbial experiment process can not pollute the external environment, and the influence of the external environment on the experiment process is prevented.
The gas-liquid storage assembly comprises a storage pool 2 and a turnover membrane 3, wherein the storage pool 2 is connected to the culture pool 1, a first accommodating cavity 4 is formed between the gas-liquid storage assembly and the culture pool 1, the transmission assembly can be installed through the first accommodating cavity 4, the structure is compact, the overall structure is miniaturized, the turnover membrane 3 is arranged in the storage pool 2, the inner cavity of the storage pool 2 is divided into a sealed liquid storage cavity 201 and a sealed gas storage cavity, and the effective isolation of the culture solution in the liquid storage cavity 201 and the gas in the gas storage cavity is realized through dividing the inner part of the storage pool 2 into two independent closed spaces.
The transmission assembly is arranged in the first accommodating cavity 4 and comprises a liquid inlet pipeline and a gas outlet pipeline, the culture pond 1 is connected with the liquid storage cavity 201 through the liquid inlet pipeline, the liquid inlet pipeline is used for conveying culture liquid from the liquid storage cavity 201 to the culture pond 1 to activate microorganism culture, the culture liquid can be distilled water, aqueous solution or nutrient solution and the like, the culture pond 1 is connected with the gas storage cavity through the gas outlet pipeline, and redundant gas in the culture pond 1 flows into the gas storage cavity through the gas outlet pipeline, so that a closed gas-liquid circulation system is formed.
Specifically, the feed liquor pipeline can be with the culture solution transmission in the stock solution chamber 201 of storage pond 2 to cultivate in pond 1, for microorganism growth provides nutrient substance, in liquid transmission, because cultivate pond 1 and storage pond 2 are seal structure, the inside pressure increase of pond 1 cavity is cultivateed to the culture solution, thereby make the gas in the pond 1 independently flow into the gas storage intracavity of storage pond 2 through the gas outlet line, oppression upset membrane 3 takes place deformation upset, and then the pressure in the pond 1 cavity is cultivateed in the relief, in order to guarantee the inside pressure balance of whole device, effectively improve experimental security.
It can be understood that the design of the turnover membrane 3 skillfully utilizes the liquid pressure, and when the pressure is generated by increasing the gas in the gas storage cavity, the turnover membrane 3 is pressed to deform, so that the autonomous pressure relief is realized, and the pressure balance in the whole device is maintained.
The on-orbit minitype integrated microorganism test device provided by the embodiment of the utility model can effectively control the on-orbit microorganism culture experiment process by taking the culture solution in the conveying storage pool 2 as the activation condition of microorganism culture in the culture pool 1, and can automatically remove the pressure in the device by the turnover membrane 3 without additional operation and auxiliary equipment, thereby simplifying the structure of the device, being beneficial to miniaturization of the whole structure, improving the operation convenience, realizing the gas-liquid circulation balance requirement in the rigid sealing device and further ensuring the safety.
The utility model can automatically culture microorganisms under microgravity environment by integrated design, does not need additional operation equipment, reduces complexity and risk, and has the characteristics of small integration, thereby being beneficial to saving precious space resources of a spacecraft, ensuring the safety and convenience of experiments and having great significance for on-orbit microorganism research.
According to an embodiment of the present utility model, referring to fig. 3, the turning film 3 is provided with a protrusion 301 protruding toward the direction of the gas storage chamber, and the protrusion 301 may be a hemispherical surface having an arc surface. When the gas in the gas storage cavity presses the protruding portion 301, the protruding portion can be deformed and overturned, and the protruding portion is completely recessed to one side of the gas storage cavity 201.
According to the embodiment of the utility model, the bulge 301 of the hemispherical structure protruding towards the direction of the gas storage cavity is arranged on the turnover membrane 3, so that the pressure relief performance of the turnover membrane 3 is enhanced by the special ingenious design, and a certain amount of gas can be generated along with the growth and propagation of microorganisms in the culture pond 1 in the actual operation process. When the gas pressure increases to some extent, it acts on the protrusion 301 of the turning film 3. Because the convex part 301 adopts a hemispherical design, the convex part has good response to the pressure of gas, and can more effectively convert the received pressure into deformation kinetic energy, so that the convex part 301 can flexibly concavely overturn towards one side of the liquid storage cavity 201.
The deformation overturning action can rapidly release redundant gas in the culture pond 1, thereby achieving the purpose of rapid and complete internal pressure balance adjustment and effectively preventing the safety problem possibly caused by overlarge pressure of the device. Meanwhile, the design is beneficial to ensuring the stability of the microorganism culture environment and the accuracy of experimental results, and the utility model reflects the fine consideration of the design of the microorganism test device in the microgravity environment.
According to one embodiment of the utility model, the turning film 3 is preferably a silica gel turning film.
In the embodiment of the utility model, the silica gel turning film is selected as a part of the device and has the following characteristics:
1. The material characteristics are that the silica gel is a high polymer elastomer material, and has good high temperature resistance, low temperature performance and good chemical stability. It can maintain stable physical properties in a wide temperature range, and is suitable for extreme temperature changes in space environment.
2. The silica gel turning film has excellent flexibility and elasticity, can flexibly deform and reset according to pressure change even in microgravity environment, ensures deformation turning pressure relief when the gas pressure in a storage pool is increased, and simultaneously can ensure difficult breakage or fatigue failure in long-term use.
3. The silica gel material has excellent air tightness and liquid tightness, can be used as a turnover film to effectively prevent cross contamination of gas, liquid and external environment in the culture pond, and ensures the sealing integrity of the whole test device.
According to one embodiment of the present utility model, as shown with reference to FIGS. 1-3, the culture assembly further comprises a heating film 5, a plurality of insulating blocks 6, a temperature sensor, and an electrical connector 7. The heating film 5 is coated on the outer wall of the culture pond 1 and used for heating the cavity of the culture pond to provide the temperature required by the growth of microorganisms, the plurality of heat insulation blocks 6 are arranged on the outer side of the heating film 5 at intervals to realize heat insulation and heat preservation, the temperature sensor is arranged on the outer side of the bottom of the culture pond 1 and used for detecting the temperature of the cavity of the culture pond, the electric connector 7 is arranged in the first accommodating cavity 4 and is respectively connected with the liquid inlet pipeline, the heating film 5 and the temperature sensor and used for receiving remote instructions to control the microorganism culture process, and the temperature sensor comprises real-time temperature acquisition, control of the heating film 5, control of the pump 11 and the like.
The culture assembly in the embodiment of the utility model not only comprises the culture pond 1 with a sealed design, but also realizes accurate control and automatic management of the microorganism growth environment by integrating various functional components. The special material comprises the following components and functions:
1. And the heating film 5 is attached to the outer wall of the culture pond 1, and converts electric energy into heat energy to heat the internal space of the culture pond 1, so as to ensure that a constant temperature condition suitable for the growth and propagation of microorganisms is maintained.
2. The heat insulation block 6 is arranged around the heating film 5 for reducing heat loss, ensuring effective utilization of energy, and preventing external temperature from affecting the micro-ecological environment in the culture pond 1.
3. The temperature sensor can be directly embedded or arranged at the bottom of the culture pond 1 by adopting a thermistor, can monitor and feed back actual temperature data in the cavity of the culture pond 1 in real time, ensures that the culture process is always in a preset accurate temperature control range, for example, the temperature control range is 25-37 ℃, the temperature control precision is +/-1 ℃, and meets the requirements of normal growth and sterilization treatment of microorganisms.
4. The electric connector 7 is used as a communication interface and a power supply controller, receives instructions from the controller, controls the working state of the liquid inlet pipeline (such as starting liquid injection operation of the pump 11) according to the instructions, and simultaneously adjusts the heating power of the heating film 5, reads data information of a temperature sensor and the like.
The design of the whole device of the embodiment of the utility model reflects the characteristics of high integration and intelligence, and optimizes and integrates the complex microorganism culture process in a limited space. All test steps such as liquid injection, temperature regulation and control and the like can be executed through remote instructions or automatic programs, and the direct participation of astronauts is not needed, so that the risk and difficulty of manual operation are greatly reduced, and the safety, reliability and effectiveness of an on-orbit microorganism experiment are improved. In addition, the precious space resource of the spacecraft is fully considered in the compact design, and the requirement of equipment miniaturization in the space environment is met.
According to one embodiment of the present utility model, the heating film 5 is preferably a polyimide heating film, the heating film 5 has a height of 18mm, a developed length of 90mm, and power consumption of 1W.
In the embodiment of the utility model, the polyimide material is very suitable for manufacturing heating elements in spacecrafts due to the excellent properties, such as high temperature resistance, high insulativity, high mechanical strength, good chemical stability, strong thermal stability and the like.
In the concrete parameter setting, the height of the heating film 5 is 18mm, the unfolding length is 90mm, and the dimension design can be effectively bonded and cover the outer wall of the culture pond 1, so that the heat is ensured to be uniformly distributed to the whole culture area, and the temperature condition required by the growth of microorganisms is met. The power consumption is designed to be 1W, so that the heating efficiency is ensured, the limitation of space station energy supply is considered, the purpose of low-power consumption and high-efficiency heating is realized, and the long-term stable operation and energy management of on-orbit equipment are facilitated. The precise and energy-saving design concept has important significance for experimental equipment in a space environment.
According to one embodiment of the utility model, referring to fig. 1-3, a first flange 101 and a second flange 102 are respectively arranged at two ends of the culture pond 1, a second accommodating cavity 103 is formed between the first flange 101 and the second flange 102, and the heating film 5 and the heat insulation block 6 are positioned in the second accommodating cavity 103.
The design of the culture pond 1 of the embodiment of the utility model further shows the characteristics of compact structure and miniaturization:
first turnup 101 and second turnup 102 are specially designed at the top and bottom ends of the culture pond 1, and an independent second accommodating cavity 103 is formed after the two turnups are oppositely arranged. The heating membrane 5 and the heat insulating block 6 are neatly disposed in this second housing chamber 103, and the arrangement is such that the heating assembly and the main body portion of the culture tank form a nested space utilization structure.
Through this design, on the one hand, can ensure that heating film 5 hugs closely and effectively heats cultivate pond 1, on the other hand, thermal-insulated piece 6 can prevent effectively that the heat from dispelling to the device outside, does not occupy the inside space of cultivate pond 1 again simultaneously to the validity and the stability of microorganism cultivation environment have been guaranteed. In addition, the heating component (the heating film 5 and the heat insulation block 6) is integrated in the second accommodating cavity 103 between the turnups at the end parts of the culture pond 1, so that the integration of functional modules is realized, space resources are greatly saved, the miniaturization and the light weight of the whole microorganism test device are facilitated, and the requirements of strict limitation on the volume and the weight of equipment in the space environment are met.
According to one embodiment of the present utility model, as shown with reference to FIGS. 1-3, the incubator assembly further comprises a sample holder 8, a compression frame 9, and a viewing window 10. The sample bracket 8 is detachably arranged in the culture pond 1, a fixing hole is formed in the sample bracket 8 and used for fixing a tested material sheet on the sample bracket 8 through two fixing screws, the fixing screws are M2 screws with gaskets and washers, the tested material sheet is a metal or nonmetal material sheet, the surface of the tested material sheet is inoculated with tested microorganisms, the sample bracket 8 is provided with an overflow gap 801 and is convenient for gas and liquid circulation in the culture pond 1, the compression frame 9 is detachably connected to a first flange 101 at the top end of the culture pond 1, and the window 10 is arranged between the first flange 101 at the top end of the culture pond 1 and the compression frame 9. Wherein the microorganism to be tested may be Aspergillus, penicillium, cladosporium, fusarium, staphylococcus, corynebacterium, bacillus, micrococcus and/or Pseudomonas.
In the design of the culture assembly of the embodiment of the utility model, key components such as a sample bracket 8, a compression frame 9, a window 10 and the like are further introduced to optimize the on-orbit culture experiment function of microorganisms, and the method specifically comprises the following steps:
1. The sample bracket 8 is detachably arranged in the culture pond 1, and is provided with a fixing hole in advance for firmly bearing the tested material sheet. The material sheet is typically subjected to surface inoculation with microorganisms so that the microorganisms can be subjected to growth propagation experiments under simulated space environmental conditions. The sample bracket 8 is also specially designed with an overflow gap 801, so that the free circulation of gas and liquid in the culture pond 1 is ensured, and the full contact of microorganisms and nutrient solution and the metabolic activity are facilitated.
2. The pressing frame 9 is also detachably designed and is connected to the first flanging 101 at the top end of the culture pond 1. The function of the pressing frame 9 is to firmly position the window 10 and to play a sealing role, ensure the integrity of the culture environment and prevent liquid leakage or gas escape.
3. The window 10 is arranged between the first flanging 101 at the top end of the culture pond 1 and the compression frame 9, so that scientific researchers can observe the growth condition of microorganisms on the sample bracket 8 and the change of the whole culture process through the window 10 without opening the device, thereby not only maintaining the requirement of aseptic operation, but also reducing the potential risk caused by frequent opening and closing of equipment.
The structural design ensures that the microorganism test device has high operation convenience and observation convenience, ensures the accuracy and safety of experiments, and reflects the high-efficiency utilization and scientific consideration of biological experiment equipment in microgravity environment.
According to one embodiment of the present utility model, window 10 is preferably a polycarbonate window.
In an embodiment of the present utility model, window 10 is made of a polycarbonate material. Polycarbonates are very suitable for use in the microbiological test device of the present utility model due to their unique performance characteristics, in particular as follows:
1. The polycarbonate has excellent light transmittance, can clearly observe the growth condition of microorganisms on a sample bracket 8 in the culture pond 1, and ensures the accuracy of experimental observation.
2. Compared with traditional materials such as glass, the polycarbonate has extremely high impact strength, is especially suitable for being used under the condition of being possibly influenced by special space environments such as vibration, microgravity and the like, and can effectively prevent the safety risk caused by window breakage.
3. The weight is light, the weight reduction is important as a part on a spacecraft, and the density of the polycarbonate is relatively low, so that the aim of miniaturization and light weight of equipment is fulfilled.
4. The polycarbonate has good weather resistance, can resist ultraviolet radiation in the sky and other severe environmental conditions, and ensures long-term stable operation of the window.
5. The polycarbonate is easy to process into various shapes and sizes, meets the requirement of complex space structure, and can improve the wear resistance and the scratch resistance through surface treatment.
Therefore, the embodiment of the utility model not only meets the visual requirement in the microorganism culture experiment process, but also fully considers the strict requirement on materials in the space environment by selecting the polycarbonate as the window material.
According to one embodiment of the utility model, the culture pond 1 and the compression frame 9 are preferably made of aluminum alloy materials, so that the quality of the whole test device can be remarkably reduced, and the requirements of a spacecraft on strict control of the weight of a payload are met. And after proper alloying treatment, the aluminum alloy can obtain good mechanical strength and hardness, so that the stable and reliable structure of the culture pond 1 is ensured, and the compression frame 9 can firmly fix the window 10 to prevent loosening or deformation under the microgravity environment. Meanwhile, the aluminum alloy has better corrosion resistance, and can keep a good working state in a long-time space environment.
In a specific example, screw holes are respectively formed at the four top corners of the first flanging 101, the pressing frame 9 and the window 10 at the top end of the culture pond 1, and fastening assembly is performed through four M2 screws. And in the actual process, a sealing gasket can be arranged at the joint to ensure the tightness of the culture pond 1.
According to one embodiment of the utility model, referring to FIG. 4, a first support column 104 and a second support column 105 are arranged in the culture pond 1 at intervals, the first support column 104 is provided with a connecting hole 1041, the second support column 105 is provided with a liquid inlet channel 1051, the sample bracket 8 is arranged on the first support column 104 and the second support column 105, the sample bracket 8 is connected with the connecting hole 1041 of the first support column 104 through a fastener, a liquid inlet pipeline is communicated with a flowing gap 801 of the sample bracket 8 through the liquid inlet channel 1051 of the second support column 105, and the bottom of the culture pond 1 is provided with an air outlet hole 106, and the air outlet hole 106 is connected with the air outlet pipeline.
For example, two first support columns 104 and one second support column 105 are located on opposite sides of the interior of the culture pond 1, respectively, which is advantageous in improving the stability of the sample carrier 8.
The internal design structure of the culture pond 1 in the embodiment of the utility model has the characteristics of precision and high efficiency, and is specifically as follows:
1. support column design A first support column 104 and a second support column 105 are arranged inside the culture pond 1. The first support column 104 is provided with a coupling hole 1041 for fixing the sample holder 8. The sample bracket 8 is tightly connected with the first support column 104 through fasteners such as screws, so that the sample bracket 8 and the tested material sheet are ensured to be stable and motionless in the culture process, and movement or overturning caused by the influence of microgravity environment is avoided.
2. The liquid inlet channel is designed in such a way that the second support column 105 is provided with a liquid inlet channel 1051 in addition to the supporting function, which directly communicates with the liquid inlet pipeline and the flow gap 801 on the sample holder 8. The culture solution can be directly conveyed to the sample bracket 8 from the liquid inlet channel 1051, so that microorganisms can be quickly contacted with fresh culture solution after being inoculated on the surface of a tested material sheet, and the culture efficiency and the possibility of maintaining the activity of the microorganisms are improved.
In conclusion, the design ensures effective transmission and management of liquid and gas in the microbial experiment process, realizes accurate control of the microbial growth environment, simplifies the operation flow, and reduces the complexity of microbial experiment operation on a space station. In addition, due to high integration and reasonable functional layout of the internal components of the equipment, the safety and reliability of the on-orbit operation of the whole microorganism test device are greatly improved.
According to one embodiment of the present utility model, as shown with reference to FIGS. 1-3, the inlet line basically comprises a feed tube, a pump 11, and a thermal insulation cushion 12. The liquid inlet pipe is respectively connected with liquid storage cavities 201 of the culture pond 1 and the storage pond 2, the pump 11 is clamped in the first accommodating cavity 4 between the culture pond 1 and the storage pond 2, the pump 11 is positioned in the liquid inlet pipe, and the heat insulation buffer cushion 12 is arranged between the pump 11 and the storage pond 2, namely positioned at the bottom of the pump 11.
In the embodiment of the utility model, the design of the liquid inlet pipeline fully considers the special requirements in the space environment and the accurate control requirements of the microorganism culture test, and the method comprises the following steps:
1. The liquid inlet pipe is used as a key component for liquid transmission, and two ends of the liquid inlet pipe are respectively connected to the liquid storage cavities 201 of the culture pond 1 and the storage pond 2, so that the culture liquid can be accurately conveyed to the culture pond 1 from the storage pond 2 according to experimental requirements.
2. The pump 11 is arranged in the first accommodating cavity 4 and is clamped between the culture pond 1 and the storage pond 2, so that the detachable assembly stability of the whole device is enhanced, and the pump 11 can be compactly integrated inside the system. By the operation of the pump 11, the precise control of the starting time of the microorganism culture and the flow rate of the culture solution can be realized, which is important for the on-orbit biological experiment.
3. The heat insulation cushion pad 12 is arranged between the pump 11 and the storage tank 2, and mainly plays two roles:
a. The heat insulation function is that the heat insulation buffer cushion 12 can effectively prevent heat from being transmitted to the storage pool 2 because the culture pool 1 can generate heat due to the heating film 5, so that the stored culture solution is prevented from evaporating due to heating, the temperature of the culture solution is maintained to be stable, and the test is protected from being disturbed.
B. The vibration damping effect is that microgravity conditions exist in the space environment, equipment is easy to be affected by vibration, vibration generated during the operation of the pump 11 can be absorbed and reduced by additionally arranging the heat insulation buffer pad 12, the pump 11 and accessories thereof are protected from damage, and meanwhile, the running stability and reliability of the whole device are ensured.
Therefore, the design of the liquid inlet pipeline in the embodiment realizes the organic combination of compact structure, miniaturization and functionality, and meets the requirements of the on-orbit microorganism test device on high efficiency, accuracy and safety.
According to one embodiment of the utility model, the inlet and outlet lines are latex or silicone tubes and the pump 11 is preferably a peristaltic pump.
Specifically, the latex tube and the silica gel tube have good elasticity and restoring force, the peristaltic pump can realize liquid delivery by extruding the hose during operation, and after the extrusion is stopped, the hose can quickly recover, so that continuous, uniform and controllable liquid flow output is ensured, accurate control of liquid delivery can be realized, and the peristaltic pump is very important for quantitative addition and replacement of culture solution in the microbial experiment process. The peristaltic pump has the working principle that the liquid in the hose is extruded by the roller or the sliding block to advance, the liquid is not in direct contact with any part in the pump body, the problem of cross contamination is effectively avoided, and the peristaltic pump is particularly suitable for a microorganism culture test with high cleanliness requirement. In addition, compared with a hard pipeline, the latex tube and the silica gel tube are lighter, the weight of the whole device is reduced, and the strict standard of the spacecraft on the weight reduction of the effective load is met.
Therefore, the latex tube or the silica gel tube is matched with the peristaltic pump to serve as a liquid inlet and air outlet component in the microorganism test device, so that high-efficiency and accurate liquid transmission control is realized, and special requirements under on-orbit experiment conditions are fully considered.
And, the flow rate of the pump 11 is set to 3ml/min with an accuracy of 0.1ml, the control of the pump 11 is achieved by the electrical connector 7, and the selection of the parameters is mainly based on the following several considerations:
1. The requirement of microorganism growth is that the flow rate is set to meet the requirement of nutrient supply in the microorganism growth process, so that the microorganism can obtain enough nutrients for normal metabolism and propagation. The 3ml/min flow rate provides a moderate liquid turnover rate, and neither too fast results in microorganisms not reaching the point of use of the nutrients in the culture broth nor too slow results in metabolite accumulation affecting microbial health.
2. Space environment adaptability-taking into account special conditions in space environment, such as microgravity, limited resources and the like, properly reducing the flow rate helps to save resources and reduce mechanical vibration and energy consumption generated when the device is operated.
3. Experimental accuracy control-under certain specific experimental conditions, accurate control of the liquid feed rate is required to achieve stable experimental conditions. The flow rate of 3ml/min can enable researchers to better grasp the interaction process of microorganisms and the tested material sheet, and ensure the accuracy and repeatability of experimental data.
According to one embodiment of the utility model, referring to FIG. 3, the liquid inlet pipeline further comprises a wire bundle seat 13, wherein the wire bundle seat 13 is clamped on the side surface of the first accommodating cavity 4 between the second flanging 102 at the bottom end of the culture pond 1 and the heat insulation buffer cushion 12, a plurality of first wire bundle holes 131 and second wire bundle holes 132 are formed in the wire bundle seat 13, the first wire bundle holes 131 are used for penetrating and fixing the liquid inlet pipe, and the second wire bundle holes 132 are used for penetrating and fixing the wires of the temperature sensor, the electric connector 7, the pump 11 and other parts.
In this embodiment of the present utility model, the design of the liquid inlet pipeline considers the integration of harness management and space utilization efficiency, and is specifically as follows:
1. The wire bundle seat 13 is arranged on the side surface of the first accommodating cavity 4 between the culture pond 1 and the heat insulation buffer cushion 12, and the layout is favorable for orderly integrating wire bundles such as wires, cables and the like, so that the internal structure of the device is kept compact, and the whole miniaturization is realized.
2. Wire bundle holes, namely a plurality of wire bundle holes are arranged on the wire bundle seat 13 and are used for fixing different wires respectively. One part of the wire bundle holes are specially used for serving the liquid inlet pipe, so that the liquid inlet pipe can be stably and reasonably distributed in the device, and the other part of the wire bundle holes provide threading channels for electronic components such as the temperature sensor, the electric connector 7, the pump 11 and the like, so that the electric connection among the components is ensured to be stable and reliable.
3. The wire harness seat 13 is integrated in the equipment, and special wire harness holes are distributed, so that the complexity of circuit layout is simplified, the assembly difficulty is reduced, the maintenance convenience and safety of the device are improved, and the potential safety hazard caused by mess of cables is avoided.
In summary, the design of the wire harness seat 13 in the embodiment of the utility model fully embodies the engineering optimization thought under the space environment condition with limited space, not only meets the functional requirement, but also considers the light weight and compact principle required by the spacecraft application.
According to an embodiment of the present utility model, referring to fig. 1 to 3, the left and right sides of the heat insulation cushion pad 12 are respectively provided with a fixing ring 121, and the inlet pipe and the outlet pipe are respectively and correspondingly arranged through the fixing rings 121.
Specifically, a first connecting pipe 107 and a second connecting pipe 108 are respectively arranged at the left side and the right side of the bottom of the culture pond 1, the first connecting pipe 107 is communicated with a liquid inlet channel 1051 of a second supporting column 105 in the culture pond 1, the second connecting pipe 108 is communicated with an air outlet hole 106 at the bottom of the culture pond 1, a third connecting pipe 202 communicated with a liquid storage cavity 201 and a fourth connecting pipe 203 communicated with a gas storage cavity are arranged on the storage pond 2, the first connecting pipe 107, a left fixing ring 121 and the third connecting pipe 202 are correspondingly arranged along the height direction, and the second connecting pipe 108, the right fixing ring 121 and the fourth connecting pipe 203 are correspondingly arranged along the height direction.
One end of the liquid inlet pipe is connected with the first connecting pipe 107 at the bottom of the culture pond 1, the other end of the liquid inlet pipe is connected with the third connecting pipe 202 of the liquid storage cavity 201 after penetrating through the wire bundle seat 13 and the left fixing ring 121, one end of the air outlet pipe is connected with the second connecting pipe 108 at the bottom of the culture pond 1, and the other end of the air outlet pipe is connected with the fourth connecting pipe 203 of the air storage cavity after penetrating through the right fixing ring 121.
In this embodiment of the utility model, the design of the insulation cushion 12 further embodies concerns about the stability and durability of the space apparatus, as follows:
1. Fixing ring design the left and right sides of the insulation buffer pad 12 are provided with fixing rings 121 for precisely positioning and fixing the inlet pipe and the outlet pipe. Through the mode of wearing to locate solid fixed ring 121, ensure that the pipeline is at the inside firm installation of device, avoid shifting or becoming flexible because of factors such as vibration, microgravity environment lead to.
2. The vibration prevention function is that the fixing ring 121 not only can provide a supporting point required by a pipeline, but also can effectively absorb and reduce vibration generated by pump operation or other mechanical movements, thereby protecting the pipeline connection from the influence of additional stress and enhancing the stability of the whole microorganism test device in the operation process.
3. The assembly efficiency and reliability are improved, namely the positioning and fixing of the pipeline are realized by utilizing the fixing ring 121, the assembly steps are simplified, and the convenience and accuracy of the whole assembly are improved. Meanwhile, the sealing performance of the pipeline connecting part is enhanced by the structural design, liquid or gas leakage is prevented, and the reliability and the safety of experimental data are ensured.
Therefore, the unique design in the detail is of great importance for optimizing the overall efficiency of the on-orbit microorganism test device and ensuring the long-term stable operation of the on-orbit microorganism test device.
According to an embodiment of the present utility model, referring to fig. 1 to 3, a connection frame 14 is provided between the heat insulation buffer pad 12 and the storage tank 2, the connection frame 14 is detachably connected with the storage tank 2, and a plurality of connection columns 109 are provided at intervals at the bottom edge of the culture tank 1, and the connection columns 109 are detachably connected with the connection frame 14 through the heat insulation buffer pad 12, so that a first accommodating chamber 4 can be formed between the culture tank 1 and the heat insulation buffer pad 12.
For example, the four corners of the cushion pad 12 are provided with the first connecting portions 122, the four corners of the connecting frame 14 are provided with the second connecting portions 141, the four corners of the cover 205 of the storage pool 2 are provided with the third connecting portions 206, and the bottom four corners of the culture pool 1 are provided with the connecting posts 109, respectively. During assembly, the second connecting portion 141 of the connecting frame 14 is fastened to the third connecting portion 206 of the lid 205 of the storage pool 2 by screws, and the second connecting portion 141 of the connecting frame 14 and the first connecting portion 122 of the heat insulating cushion pad 12 are simultaneously connected to the connecting column 109 at the bottom of the culture pool 1 by screws penetrating from bottom to top.
In this embodiment of the present utility model, the connection frame 14 is used as an important connection structure between the culture tank 1 and the storage tank 2 to realize multiple functions, specifically as follows:
1. The connection frame 14 not only fixes the heat insulation buffer pad 12 in place, but also plays a role of connection and support, so that the connection between the storage pool 2 and the culture pool 1 is detachable. This design facilitates installation, maintenance and replacement of the device.
2. The first accommodation chamber 4 is constructed by providing a plurality of connection posts 109 at the bottom edge of the culture pond 1 and detachably connecting with the connection frame 14 via the heat insulation buffer pad 12, thereby forming the first accommodation chamber 4. The key components such as the pump 11 and the like can be placed in the accommodating cavity, so that the structure is compact, and the miniaturization of the whole structure is facilitated.
3. The heat insulation and buffering can be realized through the heat insulation buffer pad 12, and the connecting frame 14 is positioned between the heat insulation buffer pad 12 and the storage tank 2, so that the heat insulation buffer pad has a physical connection function, more importantly, the heat insulation buffer pad can further effectively isolate a heat source, prevent heat from being conducted to the culture solution in the storage tank 2, and keep the temperature of the culture solution stable. Meanwhile, the device can absorb mechanical vibration possibly generated, protect the internal precise components of the device from damage, improve the stability and service life of the whole microorganism test device, and realize secondary heat insulation and buffering.
Therefore, the structural design fully considers the special requirements in the space environment, ensures flexible and convenient assembly among all components, and also meets the requirements of heat management and vibration reduction, thereby having significant significance for improving the overall performance of the on-orbit microorganism experimental equipment.
According to one embodiment of the utility model, the connecting frame 14 is provided with a number of lightening holes 142 to reduce the overall mass.
According to one embodiment of the utility model, the volume of the storage pool 2 can be 3ml, the storage pool 2 comprises a pool body 204 and a cover body 205 which are detachably connected through four screws, a turnover membrane 3 is arranged between the pool body 204 and the cover body 205, a liquid storage cavity 201 is formed between the pool body 204 and the turnover membrane 3, and a gas storage cavity is formed between the cover body 205 and the turnover membrane 3.
The tank 204 is provided with a third connection pipe 202 communicating with the liquid storage chamber 201, and the cover 205 is provided with a fourth connection pipe 203 communicating with the liquid storage chamber.
According to one embodiment of the utility model, the cavity of the culture pond 1 is a cylindrical cavity with the inner diameter of 30mm and the depth of 20mm, the material is aluminum alloy, a space is provided for microorganism culture, the space volume is about 14ml, a proper amount of microorganism culture solution and a tested material sheet can be contained, and the requirement of a specific microorganism experiment on the culture environment capacity is met. Meanwhile, the compact design is beneficial to miniaturization and integration of the whole microorganism test device.
According to one embodiment of the present utility model, referring to fig. 3, the number of the heat insulation blocks 6 is four, and the heat insulation blocks 6 are respectively arranged at the four top corners of the culture pond 1, and each heat insulation block 6 is in the shape of an isosceles trapezoid, and the inner side of each heat insulation block is an arc surface matched with the cylindrical culture pond 1, so that the outer side surface of each heat insulation block 6 is tangent to the outer wall of the cylindrical culture pond 1 to form a square body.
By means of the shape design, the heat insulation block 6 can be tightly attached to the outer wall of the cylindrical culture pond 1, good contact area is ensured, the influence of the external environment on the temperature in the culture pond can be effectively prevented, heat loss is reduced, and meanwhile potential safety risks caused to external equipment or astronauts by high temperature in the culture pond 1 are prevented.
The heat insulation block 6 adopts an isosceles trapezoid structure tangent to the outer wall of the culture pond 1, and forms a square shape wrapping a part of the culture pond 1, so that the utilization rate of the space of the device is improved, the purposes of miniaturization and compactness are achieved, and the heat insulation block is particularly important for a limited space station environment.
In summary, the design of the heat insulation block 6 not only meets the requirement of the on-orbit microorganism experimental device on efficient heat insulation, but also fully considers the requirement of the space environment on the limitation of equipment volume and weight, and reflects the characteristics of high integration level and optimal design.
According to one embodiment of the present utility model, referring to fig. 1, the culture assembly, the transfer assembly and the gas-liquid storage assembly of the microorganism test apparatus of the present utility model are stacked in the height direction, and the overall apparatus has a shape of a rectangular parallelepiped of approximately 60mm (length) 60mm (width) 900mm (height), that is, a volume of only 324cm 3, so that a parallel test can be performed on a plurality of apparatuses simultaneously in a limited space.
The working principle of the on-orbit small-sized integrated microorganism testing device provided by the utility model is described below with reference to specific examples, and the on-orbit small-sized integrated microorganism testing device mainly comprises:
1. And controlling the process of the microorganism culture experiment, wherein before the microorganism culture experiment is started, the inoculated microorganism is dry spores of the microorganism, and the microorganism is in a dormant state and does not grow and reproduce. When the culture needs to be started, the transportation culture solution is used as an activation condition, the temperature control function is started at the same time, and after the microorganism has a growth condition, the microorganism starts to grow and reproduce.
2. In the liquid conveying working process, the liquid is conveyed by a micro peristaltic pump, after the micro peristaltic pump is started, the culture solution flows out of the liquid storage cavity 201 of the storage tank 2 through a liquid inlet pipe and the micro peristaltic pump and finally reaches the cavity of the culture tank 1, so that nutrient substances are provided for the growth of microorganisms. When the liquid is conveyed, as the culture pond 1 and the storage pond 2 are of sealing structures, the pressure in the cavity of the culture pond 1 can be increased, and the gas in the cavity of the culture pond 1 can automatically flow into the gas storage cavity of the storage pond 2 through the gas outlet pipeline, so that the silica gel overturning film is pressed to deform and overturn, the pressure in the cavity of the culture pond 1 is removed, and the balance of the pressure in the whole device is ensured. After the test, the storage pool 2 is disassembled, and the silica gel turning film can be observed to be completely sunken to one side of the liquid storage cavity 201.
The control method of the in-orbit miniature integrated microorganism test device provided by the utility model is continuously described below, and the control method of the in-orbit miniature integrated microorganism test device described below and the in-orbit miniature integrated microorganism test device described above can be correspondingly referred to each other.
According to an embodiment of the present utility model, referring to fig. 5, the present utility model further provides a control method of the on-orbit small-sized integrated microorganism test apparatus according to the above embodiment, which mainly includes the following steps:
S501, under the condition of microgravity sealing, acquiring a test starting instruction;
S502, responding to a test starting instruction, and controlling a liquid inlet pipeline to transmit the culture liquid in the liquid storage cavity 201 of the storage pool 2 into the culture pool 1;
Under the pressure effect of the culture solution, the gas in the culture pond 1 automatically flows into the gas storage cavity of the storage pond 2 through the gas outlet pipeline, and the turnover membrane 3 is pressed to deform and turn.
The control method provided by the embodiment of the utility model aims at the automatic starting and pressure balance adjusting flow of the on-orbit small-sized integrated microorganism test device in the microgravity sealing environment. The method comprises the following steps:
First, a start command to start a microorganism culture test is obtained by a remote command or a preset program under conditions that ensure that the whole device is under microgravity and completely sealed.
When receiving the test start instruction, the pump 11 of the automatic control liquid inlet pipeline starts to work, and the microorganism culture liquid reserved in the storage pool 2 is accurately transmitted to the inside of the culture pool 1, so that necessary nutrition environment is provided for microorganism growth.
Along with the injection of the culture solution into the culture pond 1, the gas originally existing in the culture pond 1 is extruded due to the fact that the liquid occupies space and generates certain pressure, and naturally flows out into the gas storage cavity of the storage pond 2 through the gas outlet pipeline. The process skillfully utilizes the fluidity principle of the gas in the closed system, and realizes the autonomous exhaust function without external power driving.
In the process, the turnover membrane 3 serves as a key pressure regulating component, and deforms and turns along with the increase of the gas pressure in the gas storage cavity, so that the pressure relief effect is achieved, the pressure balance in the whole device is maintained, damage to equipment caused by overpressure is prevented, and meanwhile, microorganisms can grow and reproduce normally in a stable environment.
In conclusion, the control method realizes the automatic starting and self-adaptive pressure management of the microorganism culture experiment in the on-orbit environment through the intelligent operation flow, embodies the highly integrated and refined design thought, and greatly improves the safety and efficiency of the space biological experiment.
In example 1, the test material sheet is a metal material, the test microorganism is a fungus, and the inside of the liquid storage cavity of the storage pool is sterile water.
A material microorganism corrosion test was performed using Aspergillus niger (Aspergillus niger) as a test microorganism and an aluminum alloy sheet as a test material sheet. Firstly, the prepared aspergillus niger spores are fixed on the surface of an aluminum alloy sheet in a spraying mode, and after natural drying, a tested material sheet is fixed on a sample bracket. The cavity of the culture pond is in a dry state initially, and the aspergillus niger spores are in a dormant state and cannot germinate. When the corrosion test needs to be started, the micro peristaltic pump is started, and sterile water flows out of the liquid storage cavity of the storage tank through the liquid inlet pipe and finally reaches the cavity of the culture tank. And then the whole culture pond cavity is changed into a humid environment through natural evaporation, and meanwhile, a polyimide heating film is started to control the temperature to be about 28 ℃. Environmental conditions cause Aspergillus niger spores to start to germinate, start to grow on the surface of the aluminum alloy sheet, and start the corrosion test. The results of the 90-day test show that the Aspergillus niger can uniformly grow on the surface of the aluminum alloy sheet.
In example 2, the test material layer is a polymer material, the test microorganism is fungus, and the inside of the liquid storage cavity of the storage pool is nutrient solution.
A material microorganism corrosion test was performed using Aspergillus niger (Aspergillus niger) as a test microorganism and a polyimide sheet as a test material sheet. Firstly, the prepared aspergillus niger spores are fixed on the surface of a polyimide sheet in a spraying mode, and after natural drying, the material is fixed on a sample bracket. The cavity of the culture pond is in a dry state initially, and the aspergillus niger spores are in a dormant state and cannot germinate. When the corrosion test needs to be started, the micro peristaltic pump is started, and the nutrient solution flows out of the liquid storage cavity of the storage pool through the liquid inlet pipe and finally reaches the cavity of the culture pool. And then the whole cavity of the culture pond is changed into a humid environment through natural evaporation, and meanwhile, a polyimide heating film is started to control the temperature to be about 32 ℃. Environmental conditions aspergillus niger spores began to germinate, began to grow on the polyimide sheet surface, and the corrosion test started. The results of the 60-day test show that Aspergillus niger can uniformly grow on the surface of the polyimide sheet.
The description of the modular space microbiological test load provided by the utility model is continued below, and the test load adopts the test device described in the embodiment.
According to an embodiment of the second aspect of the present utility model, and as shown with reference to FIGS. 6-11, the present utility model also provides a modular spatial microbiological test load consisting essentially of a housing, a plurality of test devices and a controller 15. The device comprises a shell, a plurality of observation holes, a plurality of test devices and a controller, wherein the front side of the shell is provided with the plurality of observation holes, the plurality of test devices are distributed in the shell in an array mode and correspond to the observation holes, the test devices are used for in-situ tests of microorganisms in an on-orbit mode, and the controller 15 is arranged on the shell and is electrically connected with the test devices and used for independently controlling the microorganism test process of each test device.
Specifically, by adopting a modularized structural layout, a plurality of small independent test devices are integrated in a shell in an array distribution mode, and the modularized design greatly improves the space utilization rate and allows the number of the test devices to be flexibly increased or decreased according to research requirements.
Meanwhile, the test device is ensured to have corresponding observation holes, so that the growth state of microorganisms in each device can be monitored in real time, and visual and various experimental data are provided.
And, can adjust and control parameters such as temperature, intake volume in each test device independently through controller 15, realize the microorganism synchronous cultivation under the different conditions of multiple different kinds or same species.
Therefore, compared with the traditional single culture mode, the utility model has high throughput culture capability, can realize mutually independent control among a plurality of test devices, simultaneously carries out a plurality of microorganism tests, remarkably improves test efficiency and throughput, and is beneficial to shortening the research period. The test load of the utility model is high-flux microorganism experimental equipment applied to space environment.
According to one embodiment of the present utility model, referring to fig. 6 and 7, the housing includes a first frame 161, a front cover 162 and a rear cover 163, the front side of the first frame 161 is provided with a plurality of first viewing holes 164 corresponding to the test devices one by one, and the first frame 161 is detachably connected to the test devices, the front cover 162 is detachably connected to the front side of the first frame 161, and the front cover 162 is provided with a plurality of second viewing holes 165 corresponding to the first viewing holes 164 one by one, i.e., the first viewing holes 164 and the second viewing holes 165 are also distributed in an array, and the rear cover 163 is detachably connected to the rear side of the first frame 161.
For example, a plurality of screw holes are formed around the first observation hole 164 of the first frame 161, and are connected to the pressing frame 9 at the top end of the test device by screws, and a plurality of screw holes are formed at the edges of the front cover plate 162 and the rear cover plate 163, respectively, and the front cover plate 162, the rear cover plate 163 and the first frame 161 are fixed by screws.
In this embodiment of the present utility model, the first frame 161 is detachably connected to the test devices, so that each test device can be flexibly installed, replaced or maintained according to actual requirements.
The front cover plate 162 is detachably connected to the front side of the first frame 161, and is provided with second observation holes 165 corresponding to the first observation holes 164 one by one, so that an observation channel is formed, allowing the user to clearly see the culture process from the outside without opening the front cover plate 162, and ensuring the stability and sterility of the experimental environment. The back cover 163 is also removably attached to the back side of the first frame 161 to provide closed protection for the back of the entire apparatus.
The first observation holes 164 and the second observation holes 165 are distributed in an array, and the layout characteristics not only meet the requirement of high-flux multi-unit parallel experiments, but also ensure the independence and mutual noninterference among units, thereby being beneficial to researchers to synchronously conduct microorganism culture research under various conditions.
Therefore, the shell design fully considers the requirements of quick installation, convenient operation and efficient utilization of space resources in the space environment, and greatly improves the flexibility and practicability of the on-orbit microorganism test through a modularized assembly mode and optimized observation window configuration.
According to an embodiment of the present utility model, referring to fig. 7, a first sealing ring 166 is provided at a junction of the first frame 161 and the front cover plate 162 and a junction of the first frame 161 and the rear cover plate 163, respectively, and a first transparent viewing plate 167 is provided between the front cover plate 162 and the first frame 161.
Specifically, the first sealing ring 166 is made of a high polymer material, and corresponding grooves are respectively formed in the front and rear edges of the first frame 161 and the edges of the front cover plate 162 and the rear cover plate 163, and the first sealing ring 166 is embedded into the grooves, so that the overall sealing performance of the assembled shell can be ensured, the influence of the external environment on the internal microorganism culture test can be effectively prevented, and meanwhile, the leakage of culture solution or gas can be avoided.
The first transparent observation plate 167 is made of a high-transparency polymer material, and the growth of microorganisms in each test device can be clearly observed through the first transparent observation plate 167.
The design meets the requirements of experimental observation and maintains the sealing integrity of the equipment. The effect of high integration and integral sealing of multiple samples in a compact space is achieved.
In addition, the front cover 162, the first frame 161, the rear cover 163, and the like are made of high-strength materials such as aluminum alloy, titanium alloy, carbon fiber, and the like, for improving the overall structural strength.
The front cover 162 is designed to reduce weight, and the first frame 161, the rear cover 163 and other parts can be provided with first reinforcing ribs 168, so that the overall strength and rigidity of the shell are ensured while the weight of the shell is reduced.
Unlike the above embodiment, referring to fig. 8-11, the housing provided in the embodiment of the present utility model is of a quick-insertion design, and mainly includes a second frame 171 and a plurality of module cavity shells 172, wherein a plurality of mounting ports 173 are arranged side by side on the front side of the second frame 171, the plurality of module cavity shells 172 are disposed in the second frame 171 and detachably mounted on the mounting ports 173 through screws in a one-to-one correspondence manner, so as to realize quick assembly and disassembly, a plurality of test devices are disposed in each module cavity shell 172, and observation holes corresponding to the test devices are disposed on the front side of each module cavity shell 172 in a one-to-one correspondence manner.
The embodiment of the utility model can realize the rapid installation or disassembly of a plurality of groups of standardized test modules under the limited space and weight constraint conditions. Each standard test module comprises a plurality of small-sized and independent microorganism test devices, all the devices are completely isolated, the liquid supply and the temperature of the devices are independently controlled, the independence and the undisturbed performance of each test are ensured, and a plurality of microorganism tests can be simultaneously operated.
Therefore, the design concept of the quick-plug type microorganism test module is to realize standardization, modularization and efficient resource utilization, greatly improves the operation flexibility of the experiment and is beneficial to accelerating the experiment process.
According to one embodiment of the present utility model, referring to fig. 10 and 11, the module chamber housing 172 is substantially rectangular in shape and includes a chamber housing 174, a front side plate 175 and a rear side plate 176, the front side of the chamber housing 174 is provided with a plurality of third observation holes 177 in one-to-one correspondence with the test devices, the front side plate 175 is detachably connected to the front side of the chamber housing 174, the front side plate 175 is provided with a plurality of fourth observation holes 178 in one-to-one correspondence with the third observation holes 177, and the rear side plate 176 is detachably connected to the rear side of the chamber housing 174. By the design, each test device can be flexibly installed, replaced or maintained according to actual requirements.
According to an embodiment of the present utility model, referring to fig. 11, a second sealing ring 179 is provided at the junction of the cavity case 174 and the front side plate 175 and the junction of the cavity case 174 and the rear side plate 176, respectively, and a second transparent viewing plate 180 is provided between the front side plate 175 and the cavity case 174.
Specifically, the second sealing ring 179 is made of a high polymer material, and corresponding grooves are respectively formed in the front and rear edges of the cavity shell 174 and the edges of the front side plate 175 and the rear side plate 176, and the second sealing ring 179 is embedded into the grooves, so that the overall sealing performance of the assembled module cavity shell 172 can be ensured, the influence of the external environment on the internal microorganism culture test can be effectively prevented, and meanwhile, the leakage of culture solution or gas can be avoided.
And, the second transparent observation plate 180 is made of high transparent high polymer material, and the growth of microorganisms in each test device can be clearly observed through the second transparent observation plate 180. The design meets the requirements of experimental observation and maintains the sealing integrity of the equipment.
In addition, the front side plate 175, the chamber housing 174, the rear side plate 176, and the like are made of high-strength materials such as aluminum alloy, titanium alloy, carbon fiber, and the like, for improving the overall structural strength.
The second frame 171 may further be provided with second reinforcing ribs 181 to secure overall strength and rigidity.
According to one embodiment of the utility model, referring to fig. 6-9, the bottoms of the left and right opposite sides of the shell are respectively provided with a mounting base 19, the mounting bases 19 are used for being mounted on corresponding parts of the spacecraft through loosening-free screws, at least one mounting base 19 is provided with a socket 20, and the socket 20 is internally provided with a controller 15.
It will be appreciated that the controller 15 may be replaced by an electrical connector, which may be a sealable connector, capable of ensuring a seal at the interface location, and implementing signal transmission control with a remote control system.
It should be noted that the above-mentioned embodiments are merely for illustrating the technical solution of the present utility model, and not for limiting the same, and although the present utility model has been described in detail with reference to the above-mentioned embodiments, it should be understood by those skilled in the art that the technical solution described in the above-mentioned embodiments may be modified or some technical features may be equivalently replaced, and these modifications or substitutions do not make the essence of the corresponding technical solution deviate from the spirit and scope of the technical solution of the embodiments of the present utility model.

Claims (10)

1. A modular spatial microbiological test load comprising:
A housing, a plurality of observation holes being provided on a front side of the housing;
The test devices are distributed in the shell in an array manner and correspond to the observation holes, and the test devices are used for in-situ tests of microorganisms;
And the controller is arranged on the shell, is electrically connected with the test device and is used for independently controlling the microorganism test process of each test device.
2. The modular space microbiological test load of claim 1 wherein the housing includes:
The front side of the first frame is provided with a plurality of first observation holes which are in one-to-one correspondence with the test devices, and the first frame is detachably connected with the test devices;
the front cover plate is detachably connected to the front side of the first frame and is provided with a plurality of second observation holes which are in one-to-one correspondence with the first observation holes;
and a back cover plate detachably connected to the back side of the first frame.
3. The modular space microbiological test load of claim 2, wherein a first seal is provided at the junction of the first frame and the front cover plate and the junction of the first frame and the rear cover plate, respectively;
and a first transparent observation plate is arranged between the front cover plate and the first frame.
4. The modular space microbiological test load of claim 1 wherein the housing includes:
The front side of the second frame is provided with a plurality of mounting openings in parallel;
The module cavity shells are arranged in the second frame and detachably mounted at the mounting ports in a one-to-one correspondence mode, a plurality of test devices are arranged in the module cavity shells, and observation holes corresponding to the test devices in a one-to-one correspondence mode are formed in the front sides of the module cavity shells.
5. The modular space microbiological test load of claim 4 wherein the module chamber housing comprises:
The front side of the cavity shell is provided with a plurality of third observation holes which are in one-to-one correspondence with the test devices;
the front side plate is detachably connected to the front side of the cavity shell, and is provided with a plurality of fourth observation holes which are in one-to-one correspondence with the third observation holes;
and the rear side plate is detachably connected to the rear side of the cavity shell.
6. The modular space microbiological test load of claim 5 wherein a second seal ring is provided at each of the junction of the housing and the front side plate and the junction of the housing and the rear side plate;
and a second transparent observation plate is arranged between the front side plate and the cavity shell.
7. The modular space microbiological test load of claim 1, wherein the bottoms of opposite sides of the housing are each provided with a mounting base for mounting to a spacecraft;
and at least one mounting base is provided with a socket, and the controller is arranged in the socket.
8. The modular space microbiological test load of any of claims 1-7 wherein the test device comprises:
A culture assembly comprising a sealed culture pond;
The gas-liquid storage assembly comprises a storage pool and a turnover membrane, wherein the storage pool is connected with the culture pool, and a first accommodating cavity is formed between the storage pool and the culture pool;
the transmission assembly is arranged in the first accommodating cavity and comprises a liquid inlet pipeline and a gas outlet pipeline, the culture pond is connected with the liquid storage cavity through the liquid inlet pipeline, and the culture pond is connected with the gas storage cavity through the gas outlet pipeline.
9. The modular space microbiological test load of claim 8 wherein the flip membrane is provided with a projection projecting toward the gas storage chamber.
10. The modular space microbiological test load of claim 8, wherein the culture assembly further comprises:
the heating film is coated on the outer wall of the culture pond;
the heat insulation blocks are arranged on the outer side of the heating film in a surrounding mode at intervals;
The temperature sensor is arranged in the culture pond;
The electric connector is arranged in the first accommodating cavity and is respectively connected with the controller, the liquid inlet pipeline, the heating film and the temperature sensor.
CN202420823219.0U 2024-04-19 2024-04-19 Modular Space Microbial Experimental Payload Active CN222250743U (en)

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