CN221777881U - A cell selection device - Google Patents

Abstract

The application discloses a cell selecting device, which relates to the field of experimental equipment, and comprises: the pipette comprises a pipette shell and an adsorption component, wherein a handle is arranged on the side part of the rear end of the pipette shell, the adsorption component comprises a piston cavity, the piston cavity is positioned at the front end of the pipette shell, a nozzle is arranged at the front end of the piston cavity, a through hole for communicating the piston cavity is formed in the nozzle, a piston is arranged in the piston cavity in a sliding mode, a piston rod is arranged in a connecting mode, the piston rod extends out of the piston cavity towards the rear end in an extending mode, a rack A is arranged at the end portion of the piston rod extending out of the piston cavity, a gear is meshed with the rack A, the gear is arranged in the pipette shell through gear shaft in a rotating mode, a button is arranged at the front end of the handle, the button is arranged in a sliding mode along the front end direction and the rear end direction, a rack B is arranged at the rear end of the button, the rack B is meshed with the gear, and a spring A is arranged at the rear end of the rack B in a precompression mode. Therefore, the problems in the experiment are solved, the operation view is prevented from being blocked, and the usability of the experimental equipment is improved.

Description

Cell selecting device

Technical Field

The application relates to the field of experimental equipment, in particular to a cell selecting device.

Background

Monoclonal antibodies are an important task in modern life science research, and have an indispensable role in the structural and functional research of genes and proteins. The monoclonal antibody is prepared by the following steps: 1. selecting an immunized animal; 2. cell fusion; 3. selectively culturing; 4. screening and cloning hybridoma positive clones; 5. preparation of monoclonal antibodies in large quantities. In the selective culture of fused cells, a large number of screens and dilutions are required. The most used in the experiment is the liquid-transferring gun, the liquid-transferring gun is one kind of liquid-transferring device, is commonly used in laboratory for transferring a small amount or a trace amount of liquid, the liquid-transferring gun with different specifications is matched with the liquid-transferring gun with different sizes, monoclonal cells are required to be selected under an inverted microscope, the liquid-transferring gun is usually adopted for selecting single cells, but the prior conventional liquid-transferring gun and the matched gun are poor in usability, inconvenient to operate in the use process, and the prior matched gun is straight, and belongs to a disposable consumable product and can be directly contacted with a liquid sample, so that the accuracy of the liquid-transferring result is affected, the analysis result is directly affected by the inclusion of external biological pollutants or dissolution, the gun and the liquid-transferring gun are always in one straight line in the use process, the visual field of operators is easy to be blocked, and the operation is affected.

Disclosure of utility model

In order to solve the problems in the experiment, the application provides a cell selection device, which can avoid shielding the operation visual field, improve the usability of experimental equipment and improve the realization accuracy.

The application provides a cell selecting device which adopts the following technical scheme.

A cell sorting apparatus comprising: pipetting gun and gun head;

The liquid-transferring gun comprises a liquid-transferring gun shell and an adsorption component, wherein a handle is arranged at the side part of the rear end of the liquid-transferring gun shell, the adsorption component comprises a piston cavity, the piston cavity is positioned at the front end of the liquid-transferring gun shell, a connector is arranged at the front end of the piston cavity, a through hole communicated with the piston cavity is formed in the connector, a piston is slidably arranged in the piston cavity, a piston rod is connected and arranged, the piston rod extends out of the piston cavity towards the rear end, a rack A is arranged at the end part of the piston rod extending out of the piston cavity, a gear is meshed with the rack A, the gear is rotatably arranged in the liquid-transferring gun shell through a gear shaft, a button is arranged at the front end of the handle, a rack B is fixedly arranged on the button in a sliding manner along the front-rear direction, the rack B is meshed with the gear, and a spring A is pre-compressed at the rear end of the rack B;

The gun head is divided into a butt joint section, a thread section and a conical head section from top to bottom, and the butt joint section is used for being sleeved on the joint nozzle.

Through adopting above-mentioned technical scheme, through the setting of handle, operating personnel can hold the handle operation liquid-transfering gun, and not the rifle body of direct holding liquid-transfering gun with the hand, make the operation of this liquid-transfering gun use more convenient, through the setting of piston cavity, utilize rack and pinion meshing cooperation, press the button and can realize driving the piston and remove, and then absorb liquid, through spring A's setting, utilize spring A to drive the piston and remove, under initial condition, spring A drives rack A and removes to the rear end direction, both drive the piston and remove to the position of keeping away from the nipple, through the setting of screw thread section in the rifle head, make the rifle head can be crooked, be difficult for sheltering from operating personnel's vision, do not influence the operation.

Optionally, a holding groove is arranged on the handle.

Through adopting above-mentioned technical scheme, through setting up on the handle and holding the groove, operating personnel's finger can be put on holding the groove, is favorable to improving user's travelling comfort, improves the use impression.

Optionally, the gear includes first gear, second gear, first gear and the coaxial fixed setting of second gear, first gear and rack A meshing cooperation, second gear and rack B meshing cooperation, the tooth ratio of first gear and second gear is greater than.

Through adopting above-mentioned technical scheme, the gear ratio of first gear and second gear is greater than 1, has both played the reduction gear effect, and the customer slows rack A's travel speed, is favorable to improving control accuracy, saves the operation dynamics of button simultaneously for press the operation lighter.

Optionally, the threaded section is proximate an upper portion of the gun head, and the volume of the gun head comprises a conical head volume.

By adopting the technical scheme, the capacity of the gun head comprises the conical head end capacity, and the capacity of the gun head is easy to change due to the fact that the threaded section can be bent, so that the capacity does not comprise the threaded section.

Optionally, this cell selection device still includes adjusting part, adjusting part includes lead screw, nut, the lead screw sets up the rear end at the pipette casing along fore-and-aft direction, the tip that the lead screw stretches out the pipette casing 1 rear end is provided with the knob, and the fixed circumference rotation setting of lead screw axial, the front end of lead screw offsets with rack A, the nut is fixed to be set up on the pipette casing, and sets up with lead screw-thread fit.

Through adopting above-mentioned technical scheme, through rotatory regulation lead screw, can make lead screw promote rack A to the front end remove, perhaps to the rear end removal, rack A also moves backward under spring A spring force effect, and then adjusts the position of piston at the piston cavity, realizes the size adjustment of piston cavity inner space, and then adjusts imbibition volume, and this regulation mode is simple.

Optionally, the cell selecting device further comprises a gun head pushing and unloading assembly, the gun head pushing and unloading assembly comprises a pushing and unloading sleeve, the pushing and unloading sleeve is sleeved outside the joint mouth, the rear end of the pushing and unloading sleeve is provided with a pushing and unloading rod, the pushing and unloading rod is arranged in a sliding mode along the piston cavity through a guide ring, a spring B is arranged between the pushing and unloading rod and the rear end of the piston cavity in a precompression mode, the rear end of the pushing and unloading rod extends out of the pipetting gun shell, and the end portion of the pushing and unloading rod is provided with a pushing and unloading button.

Through adopting above-mentioned technical scheme, through the setting of rifle head shirking the subassembly, press and shirk the button and can promote shirking the cover and remove, and then promote the rifle head that the butt joint was on the mouth and remove, with the rifle head lift off, simple structure is reliable, simple operation.

In summary, the application at least comprises the following beneficial effects:

1. The application relates to a pipette which comprises a pipette shell and an adsorption component, wherein the side part of the rear end of the pipette shell is provided with a handle, the adsorption component comprises a piston cavity, the piston cavity is positioned at the front end of the pipette shell, the front end of the piston cavity is provided with a connector, the connector is provided with a through hole communicated with the piston cavity, a piston is slidably arranged in the piston cavity, the piston is connected with a piston rod, the piston rod extends out of the piston cavity towards the rear end, the end part of the piston rod extending out of the piston cavity is provided with a rack A, a gear meshed with the rack A is arranged, the gear is rotatably arranged in the pipette shell through a gear shaft, the front end of the handle is provided with a button, the button is slidably arranged along the front end and the rear end direction, the button is fixedly provided with a rack B meshed with the gear, and the rear end of the rack B is precompressed with a spring A; through the setting of handle, operating personnel can hold the handle operation liquid-transfering gun, and not directly hold the rifle body of liquid-transfering gun with the hand for this liquid-transfering gun's operation is used more conveniently, through the setting of piston cavity, utilizes rack and pinion meshing cooperation, presses the button and can realize driving the piston and remove, and then absorbs liquid, through spring A's setting, utilizes spring force to drive the piston and remove, under initial condition, spring A drives rack A and removes to the rear end direction, both drives the piston and removes to the position of keeping away from the mouth.

2. The gun head is divided into three sections, namely a butt joint section, a thread section and a conical head section from top to bottom, wherein the butt joint section is used for being sleeved on a joint nozzle. Through the setting of screw thread section in the rifle head for the rifle head can be crooked, is difficult for sheltering from operating personnel's field of vision, does not influence the operation.

3. The adjusting component comprises a screw and a nut, wherein the screw is arranged at the rear end of a pipette shell along the front-rear direction, a knob is arranged at the end part of the screw, extending out of the rear end of the pipette shell, the screw is axially fixed and circumferentially and rotationally arranged, the front end of the screw abuts against a rack A, and the nut is fixedly arranged on the pipette shell and is in threaded fit with the screw. Through rotatory regulation lead screw, can make lead screw promote rack A to the front end remove, perhaps to the rear end remove, rack A also moves backward under spring A spring force effect, and then adjusts the position of piston at the piston cavity, realizes the size regulation in piston cavity inner space, and then adjusts imbibition volume, and this regulation mode is simple.

4. The gun head pushing and unloading assembly comprises a pushing and unloading sleeve, wherein the pushing and unloading sleeve is sleeved outside a joint, a pushing and unloading rod is arranged at the rear end of the pushing and unloading sleeve, the pushing and unloading rod is arranged in a sliding mode along a piston cavity through a guide ring, a spring B is pre-compressed between the pushing and unloading rod and the rear end of the piston cavity, the rear end of the pushing and unloading rod extends out of a liquid-moving gun shell, and a pushing and unloading button is arranged at the end part of the pushing and unloading rod. Through the setting of rifle head shirking the subassembly, press and push away the button and can promote to push away and unload the cover and remove, and then promote the rifle head that the butt joint was connected on the mouth and remove, lift off the rifle head, simple structure is reliable, simple operation.

Drawings

In order to more clearly illustrate the embodiments of the utility model or the technical solutions in the prior art, the following description will briefly explain the drawings used in the embodiments or the description of the prior art, and it is obvious that the drawings in the following description are only some embodiments of the utility model, and that other drawings can be obtained according to these drawings without inventive effort to a person skilled in the art.

FIG. 1 is a schematic top view of a cell sorter.

Fig. 2 is a schematic diagram of the matching relationship of the gear, the rack a and the rack B.

Reference numerals illustrate: 1. a pipette housing; 2. a grip; 3. a piston cavity; 4. a nozzle; 5. a piston; 6. a piston rod; 7. a rack A; 8. a button; 9. a rack B; 10. a spring A; 11. a first gear; 12. a second gear; 13. a butt joint section; 14. a threaded section; 15. a conical head section; 16. a screw rod; 17. a nut; 18. a knob; 19. pushing off the sleeve; 20. a push-off lever; 21. a guide ring; 22. a spring B; 23. the push button is pushed.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present utility model, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

The application is described in further detail below with reference to fig. 1 to 2.

The embodiment of the application discloses a cell selecting device.

Referring to fig. 1 to 2, a cell sorting apparatus includes: pipette gun and gun head.

The pipette comprises a pipette shell 1 and an adsorption component, wherein a handle 2 is arranged on the side part of the rear end of the pipette shell 1, the adsorption component comprises a piston cavity 3, the piston cavity 3 is positioned at the front end of the pipette shell 1, a connector 4 is arranged at the front end of the piston cavity 3, a through hole communicated with the piston cavity 3 is formed in the connector 4, a piston 5 is slidably arranged in the piston cavity 3, a piston rod 6 is connected and arranged on the piston 5, the piston rod 6 extends out of the piston cavity 3 towards the rear end, a rack A7 is arranged at the end part of the piston rod 6 extending out of the piston cavity 3, teeth of the rack A7 face one side close to the handle 2 and are meshed with the rack A7, the gear is arranged in the pipette shell 1 through gear shaft rotation, a button 8 is arranged at the front end of the handle 2, the button 8 slides along the front end and the rear end direction, a rack B9 is fixedly connected to the top of the button 8, the rack B9 is meshed with the gear, and a spring A10 is pre-compressed at the rear end of the rack B9.

Through the setting of piston cavity 3, utilize rack and pinion meshing cooperation, press button 8 can realize driving piston 5 and remove, and then absorb liquid, through the setting of spring A10, utilize spring force to drive piston 5 and remove, under initial condition, spring A10 drives rack A7 and removes to the rear end direction, both drives piston 5 and removes to the position of keeping away from the nipple 4.

Be provided with on the handle 2 and hold the groove, through the setting of handle 2, operating personnel can hold the handle 2 and operate the liquid-transfering gun, and not directly hold the rifle body of liquid-transfering gun with the hand for this liquid-transfering gun's operation is used more conveniently, through setting up on handle 2 and hold the groove, operating personnel's finger can put on holding the groove, is favorable to improving user's travelling comfort, improves the use impression.

The gears comprise a first gear 11 and a second gear 12, the first gear 11 and the second gear 12 are coaxially and fixedly arranged, the first gear is meshed with the rack A, the second gear is meshed with the rack B, and the gear ratio of the first gear 11 to the second gear 12 is larger than 1. The speed reducer has the function of a speed reducer, can slow down the moving speed of the rack A7, is favorable for improving the control precision, and simultaneously saves the operation force of the button 8, so that the pressing operation is lighter.

The gun head is divided into three sections from top to bottom, namely a butt joint section 13, a thread section 14 and a conical head section 15, wherein the butt joint section is used for being sleeved on the joint nozzle 4. By arranging the thread section 14 in the gun head, the gun head can be bent, the visual field of an operator is not easy to be blocked, and the operation is not influenced. The threaded section 14 is adjacent the upper portion of the gun head, the volume of the gun head comprising the volume of the conical head, the volume not comprising the threaded section 14 since the threaded section 14 is bendable and the volume is subject to change.

The cell selecting device further comprises an adjusting component, the adjusting component comprises a screw rod 16 and a nut 17, the screw rod 16 is arranged at the rear end of the pipette shell 1 along the front-back direction, a knob 18 is arranged at the end part of the screw rod 16 extending out of the rear end of the pipette shell 1, the screw rod 16 is axially fixed and circumferentially rotates, the front end of the screw rod 16 abuts against the rack A7, and the nut 17 is fixedly arranged on the pipette shell 1 and is in threaded fit with the screw rod 16. Through rotating the adjusting knob 18, the lead screw 16 can push the rack A7 to move towards the front end or move towards the rear end, the rack A7 also moves backwards under the action of the spring force of the spring A10, and then the position of the piston 5 in the piston cavity 3 is adjusted, the size of the space in the piston cavity 3 is adjusted, and then the liquid suction volume is adjusted.

The cell selecting device further comprises a gun head pushing and unloading assembly, the gun head pushing and unloading assembly comprises a pushing and unloading sleeve 19, the pushing and unloading sleeve 19 is sleeved outside the connector 4, a pushing and unloading rod 20 is arranged at the rear end of the pushing and unloading sleeve 19, the pushing and unloading rod 20 is arranged in a sliding mode along the piston cavity 3 through a guide ring 21, a spring B22 is arranged between the pushing and unloading rod and the rear end of the piston cavity in a precompression mode, the rear end of the pushing and unloading rod 20 extends out of the pipette shell 1, and a pushing and unloading button 23 is arranged at the end portion of the end. Through the setting of rifle head shirk subassembly, press and push away and unload button 23 can promote to push away and unload the cover 19 and remove, and then promote the rifle head that the butt joint was connected on the mouth 4 and remove, with the rifle head lift off, simple structure is reliable, simple operation.

Description of operation: firstly, an operator needs to rotate a knob 18 to adjust the measuring range according to the use requirement, selects a gun head according to the measuring range, holds a pipette handle 2 to operate, and enables a connector 4 at the front end of the pipette to be in butt joint with a butt joint section 13 of the gun head, so that aseptic operation can be carried out by aid of auxiliary tools according to actual use conditions, and the bending direction and angle of the gun head can be adjusted. Then, the pipetting operation can be carried out according to the conventional pipetting operation flow, firstly, the button 8 is pushed to the bottom at a lower speed, then the tip of the gun head is contacted with liquid, the liquid level is kept, the button 8 is slowly loosened and completely loosened, the liquid is sucked, the button 8 is pushed to the bottom at a lower speed, the button 8 is slowly loosened and completely loosened again, the liquid is sucked, the operation can be repeated for 2-3 times, the error caused by residual on the inner wall of the gun head can be reduced, the required liquid amount is obtained, the gun head is moved out of the liquid level, the suction of the liquid is realized, the gun head is moved to a position required to be discharged according to the requirement, the button 8 is slowly pressed to the bottom, the liquid is discharged, and the pipetting operation is realized. After the end, the push-off button 23 is pressed, so that the push-off sleeve 19 can be pushed to move to push out the gun head.

In the description of the present utility model, it should be understood that the directions or positional relationships indicated by the terms "side", "end", "inner", "one side", "rear", "front", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of description and to simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus should not be construed as limiting the present utility model. In the description of the present utility model, unless otherwise specified and defined, it should be noted that the term "connected" should be interpreted broadly, and for example, it may be a mechanical connection or an electrical connection, or may be a connection between two elements, or may be a direct connection or may be an indirect connection through an intermediary, and it will be understood to those skilled in the art that the specific meaning of the term may be interpreted according to the specific circumstances.

The foregoing description of the preferred embodiment of the utility model is not intended to be limiting, but rather is intended to cover all modifications, equivalents, alternatives, and improvements that fall within the spirit and scope of the utility model.

Claims (6)

1. A cell sorting apparatus comprising: pipetting gun and gun head;

The liquid-transferring gun comprises a liquid-transferring gun shell and an adsorption component, wherein a handle is arranged at the side part of the rear end of the liquid-transferring gun shell, the adsorption component comprises a piston cavity, the piston cavity is positioned at the front end of the liquid-transferring gun shell, a connector is arranged at the front end of the piston cavity, a through hole communicated with the piston cavity is formed in the connector, a piston is slidably arranged in the piston cavity, a piston rod is connected and arranged, the piston rod extends out of the piston cavity towards the rear end, a rack A is arranged at the end part of the piston rod extending out of the piston cavity, a gear is meshed with the rack A, the gear is rotatably arranged in the liquid-transferring gun shell through a gear shaft, a button is arranged at the front end of the handle, a rack B is fixedly arranged on the button in a sliding manner along the front-rear direction, the rack B is meshed with the gear, and a spring A is pre-compressed at the rear end of the rack B;

The gun head is divided into a butt joint section, a thread section and a conical head section from top to bottom, and the butt joint section is used for being sleeved on the joint nozzle.

2. The cell sorter of claim 1 wherein the grip is provided with a hand-held slot.

3. The cell sorter of claim 1 wherein the gears comprise a first gear and a second gear, the first gear and the second gear being coaxially and fixedly disposed, the first gear being in meshing engagement with rack a, the second gear being in meshing engagement with rack B, the first gear and the second gear having a gear ratio greater than 1.

4. The cell sorting apparatus of claim 1, wherein the threaded section is adjacent to an upper portion of a lance tip, and wherein the volume of the lance tip comprises a conical head volume.

5. The cell sorting device according to claim 1, further comprising an adjusting assembly, wherein the adjusting assembly comprises a screw and a nut, the screw is arranged at the rear end of the pipette housing in the front-rear direction, a knob is arranged at the end of the screw extending out of the rear end of the pipette housing, the screw is axially fixed and circumferentially rotatably arranged, the front end of the screw abuts against the rack A, and the nut is fixedly arranged on the pipette housing and is in threaded fit with the screw.

6. The cell sorting device according to claim 1, further comprising a gun head pushing assembly, wherein the gun head pushing assembly comprises a pushing sleeve, the pushing sleeve is sleeved outside the connector, a pushing rod is arranged at the rear end of the pushing sleeve, the pushing rod is arranged in a sliding mode along the piston cavity through a guide ring, a spring B is arranged between the pushing rod and the rear end of the piston cavity in a precompression mode, the rear end of the pushing rod extends out of the pipette shell, and a pushing button is arranged at the end portion of the pushing rod.