CN219730923U - An easy-to-control combined magnetic bead reaction tube - Google Patents

Abstract

The utility model is a combined magnetic bead reaction tube that is easy to control. The first plate body is set above the outer wall of the lower tube body and is flush with the opening of the lower tube body. The first plate body extends downward along one side of the tube body in the horizontal direction. The side extension is arranged, the outer side of the first plate body extension section and the one end of the second plate body are opened and closed, and the outermost upper end of the first plate body extension section is in contact with the lower side of one end of the second plate body, and the second plate body is in contact with the lower side of the second plate body. A lower pipe cover is set at a position corresponding to the opening of the pipe body, and the outer diameter is equal to the inner diameter of the opening of the lower pipe body; an upper pipe body corresponding to the position of the lower pipe cover is fixed on the second plate body, and the lower end of the third plate body is in contact with the second plate The other end of the body is fixedly connected, the upper end of the third plate body is flush with the upper tube body, one end of the fourth plate body is opened and closed with the upper end of the third plate body, and the other end of the fourth plate body is fixed on the downward side and can be inserted into the upper tube body. The magnetic body of the tube body has the same height as the upper tube body.

Description

An easy-to-control combined magnetic bead reaction tube

Technical field

The utility model relates to the field of biomedical treatment, and in particular to a combined magnetic bead reaction tube that is easy to control.

Background technique

Magnetic beads are a new type of extraction reagent that are frequently used in nucleic acid extraction, immunology, molecular purification and other reactions. Since their invention in the 1990s, they have been widely used in immune examinations, immune detection, cell separation, biological macromolecule purification and molecular purification. It has been widely used in biology.

Due to their special physical and chemical properties, magnetic beads can reduce the reaction rate in the reaction system and affect the reaction results. Adding magnetic beads during the experiment will require opening the lid during the reaction, which may not only contaminate the sample, but may also cause aerosol contamination, affecting the progress of the reaction. For biohazardous samples, the released aerosols can cause great harm to the personal health and safety of operators.

Utility model content

In view of the problems existing in the prior art, the present invention provides an easy-to-control combined magnetic bead reaction tube, which can release magnetic beads without opening the cover and realize the function of adsorbing magnetic beads.

The utility model is realized through the following technical solutions:

A combined magnetic bead reaction tube that is easy to control, including a lower tube body, an upper tube body, a first connecting piece and a second connecting piece;

The lower tube body is arranged with an opening. The first connecting member includes a first plate body and a second plate body. The first plate body is sleeved above the outer wall of the lower tube body. The openings of the first plate body and the lower tube body are flush. One plate extends horizontally from one side of the lower tube body, the second plate has a planar structure, the outside of the extension section of the first plate body and one end of the second plate body are opened and closed, and the extension section of the first plate body is open and closed. The outermost upper end is in contact with the lower side of one end of the second plate body. A cylindrical lower tube cover is provided at the position of the second plate body corresponding to the opening of the lower tube body. The outer diameter of the lower tube cover is equal to the diameter of the lower tube body opening. inner diameter, the other end of the second plate body is located on one side of the lower tube body;

An upper tube body corresponding to the position of the lower tube cover is fixed on the second plate body. The second connecting member includes a third plate body and a fourth plate body. The lower end of the third plate body is connected to the other end of the second plate body. One end is fixedly connected, the upper end of the third plate body is away from the upper tube body and the upper end of the third plate body is flush with the upper tube body, one end of the fourth plate body is opened and closed with the upper end of the third plate body, and the other end of the fourth plate body is opened and closed. A magnetic body that can be inserted into the upper tube body is fixed on one end facing downward, and the height of the magnetic body and the upper tube body is the same.

Preferably, it also includes a cylindrical upper tube cover fixed on the downward side of the other end of the fourth plate body. The height of the upper tube cover is equal to or smaller than the upper tube body. The shape of the magnetic body is such that the outer diameter is equal to the upper tube body. The inner diameter of the cylinder is fixedly inserted into the upper tube body with the magnetic body.

Furthermore, the height of the upper tube cover is smaller than the upper tube body, and the lower end of the magnetic body is located below the upper tube cover.

Preferably, the upper tube cover, lower tube cover, lower tube body, upper tube body, first connector and second connector are all made of PC plastic.

Preferably, the magnetic body is a ferrite magnet, a rare earth magnet, an alnico alloy magnet or a polymer magnetic compound.

Preferably, the height of the upper tube body is 3-8 mm.

Preferably, the number of the upper tube bodies and the magnetic bodies is the same and is several, and the several upper tube bodies are evenly arranged on the second plate body.

Preferably, the first plate body and the second plate body are of an integrated structure, and the third plate body and the fourth plate body are of an integrated structure.

Preferably, the lower end of the third plate body is perpendicular to the other end of the second plate body.

Preferably, the lower tube body includes an integrated cylindrical section, a transition section and a truncated cone section from top to bottom. The upper end outer diameter of the transition section is the same size as the lower end outer diameter of the cylindrical section. The lower end outer diameter of the transition section is the same as the truncated cone section. The outer diameters of the upper ends of the segments are the same, and the lower end of the lower tube body is hemispherical or conical. The diameter of the hemispherical or conical bottom surface is equal to the diameter of the lower bottom surface of the truncated cone segment;

The inner diameter of the cylindrical section is the same as the outer diameter of the lower tube cover.

Compared with the existing technology, this utility model has the following beneficial technical effects:

The utility model is a combined magnetic bead reaction tube that is easy to control. The cylindrical lower tube cover can be tightly inserted into the opening of the lower tube body, and the first plate body and the second plate body can contact each other when they are put together. To ensure the airtightness of the magnetic bead reaction, the other end of the second plate body is located on one side of the lower tube body to facilitate opening the lower tube cover after the reaction is completed. The fourth plate drives the magnetic body to contact the second plate after being inserted into the upper tube body. The magnetic beads required for the reaction can be adsorbed in the lower tube cover through magnetic force. Afterwards, the magnetic body is opened as needed, and the magnetic beads can participate in the reaction. , after the reaction is completed, insert the magnetic body into the upper tube body, turn the combined magnetic bead reaction tube upside down, and adsorb all the magnetic beads to complete the reaction, thus achieving the function of placing the magnetic beads and recovering them. The utility model uses magnetic bodies to control the release of magnetic beads. The lower tube cover can be used to preserve the magnetic beads. It avoids the defect of the traditional reaction device that needs to open the cover midway to add magnetic beads during the reaction process, and can effectively solve the problem of opening the lid during the reaction process. Contamination and biohazard problems caused by cover.

Description of the drawings

Figure 1 is a schematic view of the structure of the magnetic bead reaction tube according to the present invention.

Figure 2 is a schematic structural diagram of the magnetic bead reaction tube of the present invention from another perspective.

In the picture: 1-magnetic body, 2-upper tube cover, 3-lower tube cover, 4-lower tube body, 5-first connecting piece, 6-upper tube body, 7-second connecting piece.

Detailed ways

The present utility model will be further described below in conjunction with the accompanying drawings and examples, but this does not serve as a basis for limiting the present utility model.

The utility model is a combined magnetic bead reaction tube that is easy to control, including a magnetic body 1, an upper tube cover 2, a lower tube cover 3, a lower tube body 4, a first connecting piece 5, an upper tube body 6 and a second connecting piece. 7. The first connecting member 5 includes a first plate body and a second plate body, and the second connecting member 7 includes a third plate body and a fourth plate body.

Specifically, as shown in Figures 1 and 2, on the one hand, the lower tube body 4 is used to carry the reaction system and is designed with a common structure. Its upper end is an opening, and the main structure of the first plate body is a ring. The ring is set above the outer wall of the lower tube body 4 and is flush with the opening of the lower tube body 4. The ring extends in the horizontal direction to one side of the lower tube body 4. The extension section is rectangular. The second plate body is In the planar structure of the plate structure, the outer side of the extension section of the first plate body and one end of the second plate body are opened and closed, and at the same time, the outermost upper end of the extension section is in contact with the lower side of one end of the second plate body, so that the second plate body can When in contact with the first plate body, the second plate body is provided with a lower tube cover 3 at a position corresponding to the opening of the lower tube body 4. It is cylindrical and has an outer diameter equal to that of the opening of the lower tube body 4. inner diameter, so that the lower tube cover 3 can be tightly inserted into the opening of the lower tube body 4, and the other end of the second plate body is located on one side of the lower tube body 4, so that the second plate body extends outside the lower tube body 4, and is designed with a triangular Protruding for easy opening of the lower tube cover 3.

On the other hand, the magnetic beads can be of many types according to the needs of the magnetic bead method reaction. Therefore, several upper tube bodies 6 corresponding to the positions of the lower tube cover 3 are fixed on the second plate body for fixing magnetic beads of different specifications. Beads, the lower tube cover 3 stores a variety of magnetic beads of different specifications. The lower end of the third plate body is perpendicular to the upper surface of the second plate body, and the lower end of the third plate body is fixedly connected to the other end of the second plate body. The third plate body is far away from the upper tube body 6 and flush with the upper tube body 6. One end of the fourth plate body is opened and closed with the upper end of the third plate body. The other end of the fourth plate body is fixed on the downward side and can be inserted into To the magnetic body 1 of the upper tube body 6, the number of the upper tube body 6 and the magnetic body 1 is the same and several. Several upper tube bodies 6 are spaced and evenly arranged on the second plate body. At the same time, it is necessary to ensure that the magnetic body 1 The height of the upper tube body 6 is the same, so that the magnetic body 1 can contact the second plate body after being inserted into the upper tube body 6, and these magnetic beads can be adsorbed in the lower tube cover 3 through magnetic force. If more upper tube bodies 6 need to be designed, 2 to 3 rows of upper tube bodies 6 can be designed on the second plate body according to the above structure, and the specific number can be designed according to the actual size.

In order to ensure a more stable installation of the magnetic body 1, the utility model also designs an upper tube cover 2 fixed on the downward side of the other end of the fourth plate body. The upper tube cover 2 is also cylindrical, and its height is equal to or smaller than the upper tube cover 2. Tube 6. At this time, the shape of the magnetic body 1 needs to be a cylinder with an outer diameter equal to the inner diameter of the upper tube 6, so that the magnetic body 1 is fixedly inserted into the upper tube 6 and passes through the upper tube cover during the set reaction time. 2 is turned on to release the magnetic beads. In order to save materials and labor, the height of the upper tube cover 2 is designed to be smaller than the upper tube body 6 , so the lower end of the magnetic body 1 leaks out and is located below the upper tube cover 2 .

In order to facilitate the production of the above-mentioned combined magnetic bead reaction tube, the utility model selects the materials of the upper tube cover 2, the lower tube cover 3, the lower tube body 4, the first connector 5, the upper tube body 6 and the second connector 7. They are all PC plastic, that is, polycarbonate. Originally, there are many ways to realize the opening and closing of the first plate body and the second plate body, and the opening and closing of the third plate body and the fourth plate body, such as the most common hinge. Because of the above-mentioned material selection, the first plate body and the second plate body are opened and closed. As an integrated structure, it is more convenient for the third plate body and the fourth plate body to be an integrated structure, so the above-mentioned integrated structure is selected.

The specific height of the upper tube body 6 can be 3-8mm, and the specific shape of the lower tube body 4 includes a cylindrical section, a transition section and a truncated cone section of an integrated structure from top to bottom. Therefore, the outer diameter of the upper end of the transition section is equal to the outer diameter of the lower end of the cylindrical section. The same, the outer diameter of the lower end of the transition section is the same as the outer diameter of the upper end of the truncated cone section, and the lower end of the lower tube body 4 is hemispherical or conical, so the diameter of the hemispherical or conical bottom surface needs to be equal to the diameter of the bottom surface of the truncated cone section, so the cylinder The inner diameter of the segment is the same as the outer diameter of the lower tube cover 3 mentioned earlier.

The magnetic body 1 may include, but is not limited to, ferrite magnets, rare earth magnets, alnico magnets and polymer magnetic compounds.

This utility model is a combined magnetic bead reaction tube that is easy to control. When used, the following instructions will be given according to the situation:

When performing nucleic acid extraction reactions, silicone hydroxyl magnetic beads are used; when performing mRNA extraction and purification, magnetic beads coated with oligonucleotides (dT) are required on the surface; when performing RPA-CRISPR reactions, specific enzymes are required on the surface Modified magnetic beads include, first, RPA reaction system magnetic beads: magnetic beads with surface-modified recombinase, magnetic beads with surface-modified single-stranded DNA binding protein (SSB), and surface-modified strand-displacement DNA polymerase magnetic beads; second, , CRISPR reaction system magnetic beads: surface-modified CRISPR-CAS12 magnetic beads and surface-modified magnetic beads with probes. These reactions require adding magnetic beads at a set time and order, and then releasing the magnetic beads according to the order required for the reaction. When the magnetic beads are not required to participate in the reaction, the magnetic beads are adsorbed back to their original position for storage to avoid the magnetic beads escaping in the system and affecting the system reaction or detection, thus improving the reaction efficiency, because the relevant content is all existing technology , so this utility model does not make a detailed description. The utility model takes the RPA-CRISPR reaction as an example to design a combined magnetic bead reaction tube. For additional or unmentioned reactions, a corresponding number of upper tube covers 2 can be designed according to the same idea.

The utility model is a combined magnetic bead reaction tube that is easy to control. Taking the RPA-CRISPR reaction as an example, the operation process is as follows:

Before starting the reaction, add the target detection strain sample to the lower tube body 4, then add 25 μL of reaction gain agent (10X), 2 μL of upstream and downstream primers (10 μM), and heat the temperature of the lower tube body 4 to 90°C and maintain it for 5 minutes. Cell lysis of target detection strains. At this time, the reaction starts. When the temperature of lower tube 4 cools down to 30°C, add RPA reaction system magnetic beads, including surface-modified recombinase magnetic beads, surface-modified single-stranded DNA binding protein (SSB) magnetic beads, and surface-modified strands. Replace the magnetic beads of DNA polymerase. At this time, open one of the upper tube lids 2 above the magnetic beads to complete the addition;

When the RPA reaction is completed, add another CRISPR reaction system including magnetic beads with surface modification CRISPR-CAS12 and magnetic beads with surface modification with probes. At this time, open the other upper tube cover 2 above the magnetic beads to complete. Add, and then insert the upper tube cover 2 into the upper tube body 6. When CRISPR is completed, magnetic beads need to be adsorbed. Just turn the combined magnetic bead reaction tube upside down, and the magnetic body 1 will adsorb all the magnetic beads to complete the reaction. , in this way, the function of placing different magnetic beads separately and recovering the magnetic beads can be achieved.

Claims (10)

1. A combined magnetic bead reaction tube that is easy to control, characterized in that it includes a lower tube body (4), an upper tube body (6), a first connecting piece (5) and a second connecting piece (7); The lower tube body (4) is arranged in an opening, and the first connecting member (5) includes a first plate body and a second plate body. The first plate body is sleeved above the outer wall of the lower tube body (4). The first plate body and The opening of the lower tube body (4) is flush. The first plate body extends horizontally to one side of the lower tube body (4). The second plate body has a planar structure. The outside of the extension section of the first plate body and the second plate body are arranged flush with each other. One end of the plate body is opened and closed, and the outermost upper end of the extension section of the first plate body is in contact with the lower side of one end of the second plate body. A cylindrical shape is provided at a position corresponding to the opening of the second plate body and the lower tube body (4). The outer diameter of the lower tube cover (3) is equal to the inner diameter of the opening of the lower tube body (4), and the other end of the second plate body is located on one side of the lower tube body (4); The upper tube body (6) corresponding to the position of the lower tube cover (3) is fixed on the second plate body. The second connecting piece (7) includes a third plate body and a fourth plate body. The third plate body The lower end of the third plate body is fixedly connected to the other end of the second plate body, the upper end of the third plate body is away from the upper tube body (6) and the upper end of the third plate body is flush with the upper tube body (6), and one end of the fourth plate body is aligned with the upper tube body (6). The upper end of the third plate body is opened and closed, and a magnetic body (1) that can be inserted into the upper tube body (6) is fixed on the downward side of the other end of the fourth plate body. The magnetic body (1) and the upper tube body (6) ) have the same height. 2. A combined magnetic bead reaction tube that is easy to control according to claim 1, characterized in that it also includes a cylindrical upper tube cover (2) fixed on the downward side of the other end of the fourth plate body. ), the height of the upper tube cover (2) is equal to or smaller than the upper tube body (6), the shape of the magnetic body (1) is a cylinder with an outer diameter equal to the inner diameter of the upper tube body (6), and the magnetic body (1) is fixedly plugged in in the upper tube body (6). 3. A combined magnetic bead reaction tube that is easy to control according to claim 2, characterized in that the height of the upper tube cover (2) is smaller than the upper tube body (6), and the lower end of the magnetic body (1) Located below the upper tube cover (2). 4. A combined magnetic bead reaction tube that is easy to control according to claim 2, characterized in that the upper tube cover (2), the lower tube cover (3), the lower tube body (4), the upper tube The body (6), the first connecting piece (5) and the second connecting piece (7) are all made of PC plastic. 5. A combined magnetic bead reaction tube that is easy to control according to claim 1, characterized in that the magnetic body (1) is a ferrite magnet, a rare earth magnet, an AlNiCo alloy magnet or a polymer. Magnetic compounds. 6. A combined magnetic bead reaction tube that is easy to control according to claim 1, characterized in that the height of the upper tube body (6) is 3-8 mm. 7. A combined magnetic bead reaction tube that is easy to control according to claim 1, characterized in that the number of the upper tube body (6) and the magnetic body (1) is the same and several. The upper tube body (6) is evenly arranged on the second plate body. 8. A combined magnetic bead reaction tube that is easy to control according to claim 1, characterized in that the first plate body and the second plate body are an integrated structure, and the third plate body and the fourth plate body are As an integrated structure. 9. A combined magnetic bead reaction tube that is easy to control according to claim 1, characterized in that the lower end of the third plate body is perpendicular to the other end of the second plate body. 10. A combined magnetic bead reaction tube that is easy to control according to claim 1, characterized in that the lower tube body (4) includes an integrated cylindrical section, a transition section and a truncated cone section from top to bottom. , the outer diameter of the upper end of the transition section is the same as the outer diameter of the lower end of the cylindrical section, the outer diameter of the lower end of the transition section is the same as the outer diameter of the upper end of the truncated cone section, the lower end of the lower tube body (4) is hemispherical or conical, The diameter of the bottom surface of the hemisphere or cone is equal to the diameter of the bottom surface of the truncated cone segment; The inner diameter of the cylindrical section is the same as the outer diameter of the lower tube cover (3).