CN218755520U - High-efficiency protein extraction device for high-speed centrifuges - Google Patents

Abstract

The utility model discloses a high-efficient protein extraction element suitable for high-speed centrifuge, centrifuging tube anchor clamps are located waste liquid receiving tank top and bottom and insert the waste liquid receiving tank, and centrifuging tube anchor clamps top is by the mouth formation a plurality of and places the hole, and centrifuging tube anchor clamps's outer wall is provided with buckle one, and waste liquid receiving tank outer wall is provided with buckle two, and waste liquid receiving tank bottom indent forms the thread connection groove. The device's buckle guarantees that centrifuge tube anchor clamps and waste liquid receiving tank can the same frequency stability centrifugation, and frosting makes centrifuge column and centrifuge tube anchor clamps contact well, guarantees centrifugal test's security in the at utmost, and the waste liquid receiving tank can accept the produced whole waste liquids of complete experiment, avoids taking off the centrifuge tube many times and falls the liquid, prevents that the waste liquid from polluting the mouth of pipe. The liquid level of the waste liquid is not contacted with the centrifugal column, so that the pollution of the waste liquid is greatly avoided, and the protein is extracted, thereby improving the yield of the target product of the experiment. The process of protein extraction experiment is simplified, and the protein extraction efficiency is improved greatly.

Description

High-efficiency protein extraction device for high-speed centrifuges

technical field

The utility model relates to the technical field of protein extraction, in particular to a high-efficiency protein extraction device suitable for high-speed centrifuges.

Background technique

Total protein can be divided into albumin and globulin, which have important physiological functions in the body. The determination of total serum protein is one of the important items in clinical biochemical tests. Serum protein has multiple functions such as maintaining normal colloid osmotic pressure and pH, transporting various metabolites, regulating the physiological effects of the transported substances and removing their toxicity, immune effects and nutritional effects. Serum total protein can not only be used for monitoring the nutritional status of the body, but also for the diagnosis and differential diagnosis of diseases.

Proteins in the extracellular matrix (ECM) participate in the unique, tissue-specific three-dimensional ultrastructure that makes up the ECM. Proteins in the ECM mainly include structural proteins such as collagen, proteins with linking or adhesion properties such as integrin fibronectin, etc. These proteins can provide structural support, tension strength and cell surface receptors due to their different structural characteristics. Attachment site and many other functions, ECM protein also organizes the microenvironment of cell survival, participates in the information transmission between cells, thus participates in and regulates the mechanical, physiological and biochemical behavior of tissue cells, including cell growth, differentiation, apoptosis, repair , regeneration, etc. In summary, the ECM is an important, dynamic, and integral component of all tissues and organs, serving as a natural scaffold for tissue and organ morphogenesis, maintenance, and post-injury reconstruction in nature, supported by ECM proteins. In preclinical and human clinical studies, ECM has been used as a biological scaffold for the reconstruction of many different tissue types.

Commonly used reagents for protein extraction: most proteins can be dissolved in water, dilute salt, dilute acid or dilute alkali solution, and a few proteins combined with lipids are soluble in organic solvents such as ethanol, acetone and butanol. Therefore, different methods can be used Solvent extraction isolates and purifies proteins. The first is the aqueous solution extraction method. The aqueous solution of dilute salt and buffer system makes the protein more stable and soluble. It is the most commonly used solvent for protein extraction. Dilute salt solution can promote the dissolution of protein, which is called salt dissolution. Add a small amount of neutral salts such as NaCl, generally at a concentration of 0.15mol/L. 0.02-0.05mol/L phosphate or carbonate isotonic saline solution is often used as the buffer. If the organic solvent extraction method is used, some proteins that bind firmly to lipids or have more non-polar side chains in the molecule can use organic solvents such as ethanol, acetone, or butanol, which have certain hydrophilicity and strong The lipophilicity is ideal for extracting lipoproteins, but it must be operated at low temperature. The butanol extraction method is superior to the extraction of some proteins that are tightly bound to lipids. It has a wide range of pH and temperature options, and is also suitable for animal, plant and microbial materials.

Centrifuge tube material: Centrifuge tubes commonly used in laboratories are made of plastic and glass. Generally speaking, glass centrifuge tubes have poor ductility and cannot withstand high speed or the speed of ultracentrifuges. The use of plastic centrifuge tubes is more common, and plastic centrifuge tubes are also It can be subdivided into PC (polycarbonate), PE (polyethylene), PP (polypropylene) and other materials, and the performance of PP material is relatively better. The plastic centrifuge tube is transparent or translucent, and the centrifugation of the sample can be seen intuitively, but it is relatively easy to deform, has poor corrosion resistance to organic solvents, and has a short service life.

At present, the combination of the receiving sleeve combined with the centrifuge column is used for protein extraction in the laboratory. This structure has a small receiving area. The residual waste liquid at the mouth of the tube will affect the subsequent operation steps, and the extract will be immersed in the waste liquid level in the collection tube, thereby affecting the centrifugation of the centrifuge column.

Utility model content

The purpose of this utility model is to overcome the problems raised in the above-mentioned background technology and provide a high-efficiency protein extraction device suitable for high-speed centrifuges, which can effectively solve the problem of low efficiency of existing protein extraction devices and easy contamination of protein samples The problem.

The purpose of this utility model is mainly realized through the following technical solutions:

A high-efficiency protein extraction device suitable for high-speed centrifuges, including a waste liquid receiving tank and a centrifuge tube clamp, the centrifuge tube clamp is located above the waste liquid receiving tank and the bottom end is inserted into the waste liquid receiving tank, and the top of the centrifuge tube clamp is close to the mouth A number of placement holes are formed, the outer wall of the centrifuge tube fixture is provided with buckle one, and the outer wall of the waste liquid receiving tank is provided with buckle two, after the centrifuge tube fixture is inserted into the waste liquid receiving tank, the buckle one can be turned over and connected to the second buckle, The bottom of the waste liquid receiving tank is recessed to form a screw connection tank. In the traditional laboratory for protein extraction, the combination of the receiving sleeve combined with the centrifuge column is used. This structure has a small receiving area. The residual waste liquid at the mouth of the tube caused by the liquid will affect the subsequent operation steps, and the extract will be immersed in the waste liquid level in the collection tube, thereby affecting the centrifugation of the centrifuge column. In order to solve this problem, a high-efficiency protein extraction device suitable for high-speed centrifuges is designed. The centrifuge tube clamp is located above the waste liquid receiving tank and the bottom end is inserted into the waste liquid receiving tank. The placement hole, the outer wall of the centrifuge tube fixture is provided with buckle one, and the outer wall of the waste liquid receiving tank is provided with buckle two. After the centrifuge tube fixture is inserted into the waste liquid receiving tank, the buckle one can be turned over and connected to the buckle two, and the waste liquid is received. The bottom of the groove is recessed to form a screw connection groove. During the centrifugation process, the waste liquid can flow down from the centrifuge column into the cylindrical waste liquid receiving tank that is connected with the buckle and coincides with the shaft of the high-speed centrifuge. The waste liquid receiving tank can accommodate all the waste liquid in a complete protein extraction experiment. waste liquid, and ensure that the waste liquid does not come into contact with the bottom surface of the centrifuge column. After the experiment, the cylindrical waste liquid receiving tank can be easily removed from the high-speed centrifuge, and all the waste liquid is poured out at one time, which simplifies the protein extraction process and reduces Target protein sample contamination.

Further, the placement holes are evenly distributed along the centerline of the centrifuge tube holder. The centrifuge tube fixture can design 22 placement holes at one time, and handle multi-tube centrifuge tube operations at one time. In order to ensure the quality of centrifugal extraction, the placement holes are evenly distributed along the centerline of the centrifuge tube holder.

Further, the centrifuge tube clamp is hollow inside and has an opening at the top, and the opening at the top is located inside the area where the placement hole is formed. With such a hollow internal structure, the wheelbase is larger during centrifugation, and the centrifugation effect is better.

Further, the inner wall of the placement hole has a frosted texture. Due to the small diameter of the protein extraction centrifuge column, the use of frosted materials can effectively stabilize the centrifugation process.

Further, the threaded connection groove is arranged along the center of the waste liquid receiving groove. The waste liquid receiving tank is a closed structure at the bottom, and is highly consistent with the shaft of the high-speed centrifuge through the threaded connection tank at the center, and the combination is firm. The volume of the waste liquid receiving tank is relatively large, and because of the existence of pores for accommodating the rotating shaft of the centrifuge, the vortex of the waste liquid can be avoided while avoiding its contact with the extracted protein.

Further, the buckle one is arranged symmetrically along the center of the centrifuge tube clamp. Buckle two is arranged symmetrically along the center of the waste liquid receiving tank. The buckle combination makes the centrifuge tube clamp and the cylindrical waste liquid receiving tank tightly combined, and driven by the rotating shaft of the high-speed centrifuge, the two maintain the same frequency and stable high speed.

In summary, compared with the prior art, the utility model has the following beneficial effects: the buckle of the device ensures that the centrifuge tube fixture and the waste liquid receiving tank can be centrifuged at the same frequency and stably, and the frosted surface makes the centrifuge column and the centrifuge tube fixture in good contact, The above guarantees the safety of the centrifuge experiment to the greatest extent, and at the same time, the enlarged receiving device, that is, the cylindrical waste liquid receiving tank, can receive all the waste liquid generated in the complete experiment, avoiding the need to remove the centrifuge tube multiple times for pouring, and prevent waste liquid Contaminate nozzle. Since the liquid level of the waste liquid in the cylindrical waste liquid receiving tank is not in contact with the centrifuge column, the waste liquid is largely avoided from polluting the extracted protein, thereby improving the yield of the target product in the experiment. Simplifies the process of protein extraction experiments and greatly improves the efficiency of protein extraction.

Description of drawings

The drawings described here are used to provide a further understanding of the embodiments of the utility model, constitute a part of the application, and do not constitute a limitation to the embodiments of the utility model. In the attached picture:

Figure 1 is a schematic structural view of a centrifuge tube clamp.

Fig. 2 is a schematic structural diagram of a waste liquid receiving tank.

Figure 3 is a top view of the centrifuge tube holder.

Fig. 4 is a top view of the waste liquid receiving tank.

Fig. 5 is a schematic diagram of the combination of the centrifuge tube clamp and the waste liquid receiving tank.

Fig. 6 is a schematic diagram of a state where buckle 1 and buckle 2 are not engaged.

FIG. 7 is a schematic diagram of a buckle-one and buckle-two fastening state.

The names corresponding to the reference signs in the accompanying drawings are:

1-centrifuge tube clamp, 2-placement hole, 3-buckle 1, 4-buckle 2, 5-waste liquid receiving tank, 6-thread connection tank.

Detailed ways

In order to make the purpose, technical solutions and advantages of the utility model clearer, the utility model will be further described in detail below in conjunction with the examples and accompanying drawings. The schematic implementation of the utility model and its description are only used to explain the utility model Novelty, not as a limitation to the utility model.

As shown in Figures 1 to 7, this embodiment includes a waste liquid receiving tank 5 and a centrifuge tube clamp 1. The centrifuge tube clamp 1 is located above the waste liquid receiving tank 5 and the bottom end is inserted into the waste liquid receiving tank 5. Several placement holes 2 are formed near the mouth of the top of the fixture 1 , and the placement holes 2 are evenly distributed along the centerline of the centrifuge tube fixture 1 . The centrifuge tube holder 1 is hollow inside and has an opening at the top, and the opening at the top is located inside the area formed by the placement hole 2 . The centrifuge tube clamp 1 is mainly used for placing the centrifuge tube, and the placement hole 2 is provided with a frosted layer to ensure the stability of the combination between the clamp and the centrifuge tube.

The outer wall of the centrifuge tube fixture 1 is provided with buckle 1 3, and the outer wall of the waste liquid receiving tank 5 is provided with buckle 2 4, and after the centrifuge tube fixture 1 is inserted into the waste liquid receiving tank 5, the buckle 1 3 can be turned over and connected with the buckle 2 4 For connection, buckle one 3 is arranged symmetrically along the center of the centrifuge tube holder 1 ; buckle two 4 is arranged symmetrically along the center of the waste liquid receiving tank 5 . The combination of buckle one 3 and buckle two 4 is used to ensure that the upper centrifuge tube fixture 1 and the lower waste liquid receiving tank 5 can rotate synchronously according to the corresponding centrifugal speed.

The bottom of the waste liquid receiving tank 5 is recessed to form a screw connection groove 6 . The threaded connection groove 6 is arranged along the center of the waste liquid receiving groove 5 . The waste liquid produced by each centrifugation can be collected by the waste liquid receiving tank 5, and the active ingredients are kept in the upper centrifuge tube, and the threaded connection groove 6 protruding upward at the bottom center of the waste liquid receiving tank 5 can coincide with the rotating shaft of the centrifuge , to ensure that the overall structure can rotate and centrifuge with the centrifuge shaft at a predetermined speed.

The waste liquid receiving tank 5 at the bottom can collect all the waste liquid containers in the centrifuge tubes displayed by the upper fixture at one time, which can significantly reduce the number of times of removing the waste liquid receiving tank 5 to dump the waste liquid, and improve the efficiency of protein extraction while avoiding protein samples. Contaminated during waste dumping.

The diameter of the upper opening of the centrifuge tube fixture 1 is about 13.0-16.0cm, the diameter of the lower opening is about 10.0-13.0cm, the vertical height between the upper and lower openings is about 1.0-2.0cm, and the side extension is a cylindrical side-shaped structure, and the upper opening is It is the upper opening of the centrifuge tube fixture 1. The diameter of the lower opening is the same as that of the upper opening. The vertical height between the upper and lower openings is about 2.5-3.5cm. A pair of arc protrusions on the side correspond to a central angle of about 30°, and the thickness of the protruding side is about 30°. 8mm, the height from the bottom of the side is about 1.0-1.5cm, the upper opening of the cylindrical waste liquid receiving tank 5 is as large as the outer ring of the lower bottom, the same length and diameter as the centrifuge tube fixture 1, and the upper opening and the lower bottom surface are vertical The distance is about 8.0-9.0cm. The hole on the bottom surface to accommodate the centrifuge shaft is located in the center of the bottom, and the side wall is threaded, which is suitable for the centrifuge shaft. The hole is equal in size and circle, with a diameter of about 1.0-1.5cm , the pore depth is about 4.0-5.0cm, the side extension is a cylindrical side structure, the lower opening is the upper opening of the waste liquid receiving tank 5, a pair of arc protrusions on the side, the size of which is the same as that of the upper fixture The size is exactly the same, the reversible buckle connected to the center of the side of the protrusion is slightly wider than the protrusion, about 10mm, and the length is about 1.5-2.0cm. The waste liquid produced by centrifugation can all fall into the waste liquid receiving tank 5 below. Since the waste liquid receiving tank 5 of this device can hold a considerable amount of waste liquid, it can significantly reduce the number of experimenters who take off the centrifuge tubes and waste liquid one by one. The number of times the receiving tank 5 performs the operation of dumping waste liquid.

Two pairs of symmetrical protrusions on the cylindrical sheet structure extending downward from the side of the upper centrifuge tube fixture 1 and the cylindrical sheet structure extending upward on the side of the waste liquid receiving tank 5 below are combined by reversible buckles to ensure that the upper and lower sides The synchronous rotation of the main structure. The junction of the body of the lower waste liquid receiving tank 5 and the extension of the side is the highest recommended dumping line of the waste liquid level of the waste liquid collecting tank.

The upwardly protruding threaded connection groove 6 at the center of the bottom of the waste liquid receiving tank 5 can coincide with the rotating shaft of the centrifuge, ensuring that the overall structure can rotate at a predetermined speed, and because the upwardly protruding threaded connection groove 6 at the bottom center of the waste liquid receiving tank 5, It can avoid the increase of waste liquid with the increase of centrifugation times, and the vortex will be generated in the waste liquid receiving tank 5 due to the higher rotational speed, so as to avoid the situation that the centrifugation is not complete due to the liquid level of the waste liquid being higher than the bottom of the centrifuge tube during the centrifugation process .

The bottom of the waste liquid receiving tank 5 is a circular plane with only one hole for accommodating the rotating shaft of the centrifuge, which ensures that the device can be stably placed on a plane structure such as a test table.

The waste liquid receiving tank 5 can hold all the waste liquid in a complete protein extraction experiment, and ensure that the waste liquid will not contact the bottom surface of the centrifuge column; after the experiment, the cylindrical waste liquid receiving tank can be easily removed from the high-speed centrifuge In this way, all the waste liquid is poured out at one time, which simplifies the protein extraction process and reduces the contamination of the target protein sample.

The cylindrical waste liquid receiving tank is a closed structure at the bottom, and the central characteristic part is highly consistent with the rotating shaft of the high-speed centrifuge, and the combination is firm.

The centrifuge tube fixture 1 contains 22 centrifuge tube columns, which can accommodate more centrifuge tubes and handle multi-tube centrifuge tube operations at one time.

The volume of the cylindrical waste liquid receiving tank is relatively large, and because of the existence of pores for accommodating the rotating shaft of the centrifuge, the vortex of the waste liquid can be avoided while avoiding its contact with the extracted protein.

The centrifuge column can be stably combined with the centrifuge tube fixture 1 due to the friction force, and the centrifuge tube fixture 1 and the cylindrical waste liquid receiving tank can be stably rotated at the same frequency as the high-speed centrifuge shaft under the action of the buckle, preventing the two from collapsing at high speed. Detachment during the centrifugation process mixes the liquid to be treated with the waste liquid and destroys the structure of the high-speed centrifuge.

The receiving sleeve combined with the centrifuge column used in the traditional experiment is replaced by a cylindrical waste liquid receiving tank that matches the shaft of the high-speed centrifuge, which expands the receiving volume, and the cylindrical waste liquid receiving tank that matches the high-speed centrifuge can be accommodated at one time There is a lot of waste liquid, which avoids removing the centrifuge column and receiving tube for pouring during the experimental operation, and the waste liquid at the mouth of the tube when the waste liquid is dumped will affect the subsequent operation steps, and at the same time avoids the extraction of the extract. In the waste liquid level in the tube, thereby reducing the impact on the centrifugation of the centrifuge column

The buckle ensures that the hollow centrifuge tube rack and the waste liquid receiving tank can be centrifuged stably at the same frequency. The frosted surface makes the centrifuge column and the hollow centrifuge tube rack in good contact, ensuring the safety of the centrifuge experiment to the greatest extent. At the same time, the enlarged receiving device is a cylinder The shaped waste liquid receiving tank can accept all the waste liquid generated in the complete experiment, avoid removing the centrifuge tube for pouring liquid many times, and prevent the waste liquid from polluting the nozzle. Since the liquid level of the waste liquid in the cylindrical waste liquid receiving tank is not in contact with the centrifuge column, the waste liquid is largely avoided from polluting the extracted protein, thereby improving the yield of the target product in the experiment. Simplifies the process of protein extraction experiments and greatly improves the efficiency of protein extraction.

The use process of the above-mentioned device is as follows: When using, select a centrifuge tube that is suitable for the size of the receiving hole of the centrifuge column and can be firmly combined, place it in the upper fixture, and strictly balance it. After the centrifuge tube is placed, the upper fixture is connected to the lower waste liquid receiving tank through the buckle on the side, and then the overall fixture is connected to the centrifuge shaft to ensure stability. Then start the centrifuge. After the first centrifugation, you can directly add the reagents for protein extraction in sequence according to the steps, and perform subsequent centrifugation operations. After all centrifugation operations are completed, or when the waste liquid in the waste liquid receiving tank needs to be dumped , and then open the buckle, remove the waste liquid receiving tank and dump the waste liquid to complete the protein extraction.

The above specific implementations have further described the purpose, technical solutions and beneficial effects of the present utility model in detail. It should be understood that the above are only specific implementations of the present utility model, and are not intended to limit the protection of the present utility model. Scope, within the spirit and principles of the present utility model, any modification, equivalent replacement, improvement, etc., shall be included in the protection scope of the present utility model.

Claims (7)

1. A high-efficiency protein extraction device suitable for high-speed centrifuges, characterized in that it includes a waste liquid receiving tank (5) and a centrifuge tube clamp (1), and the centrifuge tube clamp (1) is located above the waste liquid receiving tank (5) And the bottom end is inserted into the waste liquid receiving tank (5), the top of the centrifuge tube fixture (1) forms several placement holes (2) near the mouth, and the outer wall of the centrifuge tube fixture (1) is provided with a buckle one (3), The outer wall of the waste liquid receiving tank (5) is provided with buckle two (4), after the centrifuge tube fixture (1) is inserted into the waste liquid receiving tank (5), the buckle one (3) can be turned over and connected to the buckle two (4) , the bottom of the waste liquid receiving tank (5) is recessed to form a screw connection groove (6). 2. The high-efficiency protein extraction device suitable for high-speed centrifuges according to claim 1, characterized in that: the placement holes (2) are evenly distributed along the centerline of the centrifuge tube holder (1). 3. The high-efficiency protein extraction device suitable for high-speed centrifuges according to claim 1, characterized in that: the centrifuge tube holder (1) is hollow inside and has an opening at the top, and the opening at the top is located in the area formed by the placement hole (2) internal. 4. The high-efficiency protein extraction device suitable for high-speed centrifuges according to claim 1, characterized in that: the inner wall of the placement hole (2) is frosted. 5. The high-efficiency protein extraction device suitable for high-speed centrifuges according to claim 1, characterized in that: the threaded connection groove (6) is arranged along the center of the waste liquid receiving groove (5). 6. The high-efficiency protein extraction device suitable for high-speed centrifuges according to claim 1, characterized in that: the buckle one (3) is arranged symmetrically along the center of the centrifuge tube holder (1). 7. The high-efficiency protein extraction device suitable for high-speed centrifuges according to claim 1, characterized in that: the second buckle (4) is arranged symmetrically along the center of the waste liquid receiving tank (5).

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