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CN218382403U - PCR optical detection assembly - Google Patents

PCR optical detection assembly Download PDF

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Publication number
CN218382403U
CN218382403U CN202221359916.2U CN202221359916U CN218382403U CN 218382403 U CN218382403 U CN 218382403U CN 202221359916 U CN202221359916 U CN 202221359916U CN 218382403 U CN218382403 U CN 218382403U
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light
light beam
optical detection
dichroic mirror
pcr
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付沈斌
唐安华
张键
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Guangzhou Fuyi Medical Technology Co.,Ltd.
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Guangzhou Fuyishi Medical Technology Co ltd
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Abstract

The utility model relates to a biology and medical science detect technical field, more specifically relates to a PCR optical detection subassembly. A PCR optical detection assembly comprises a light source for emitting a first light beam, a first beam splitter and an optical detection device; the first beam splitter receives the first light beam, reflects the first light beam to form a second light beam and transmits the second light beam to form a third light beam; the second light beam irradiates the first to-be-detected piece and forms a first excitation light path, the third light beam irradiates the second to-be-detected piece and forms a second excitation light path, and the optical detection device receives and processes light of the first excitation light path and the second excitation light path. The utility model discloses in, the first light beam that the light source sent can form the two bundles of light that the direction is different after first beam splitter handles, and then can make the more awaiting measuring piece of position of light detection that a homology sent.

Description

PCR optical detection assembly
Technical Field
The utility model relates to a biology and medical science detect's technical field, more specifically relates to a PCR optical detection subassembly.
Background
PCR (Polymerase Chain Reaction), which is called Polymerase Chain Reaction for short, is a molecular biology technique for amplifying and amplifying specific DNA fragments. In modern molecular biology, the PCR technology has become a key technology of modern molecular biology experimental work and is widely applied to the fields of medicine, agriculture, inspection and quarantine and the like; real-time fluorescent Quantitative PCR (Quantitative Real time PCR) is a method for testing the total amount of products after each polymerase chain reaction cycle by using fluorescent chemicals in a DNA amplification reaction, namely a method for quantitatively analyzing a specific DNA sequence in a sample to be tested by an internal reference method or an external reference method. The real-time fluorescent quantitative PCR technology can realize the quantitative analysis of the DNA template and has important significance for molecular biology research, medical research and the like.
During the optical detection of PCR, a light source is required to irradiate a sample solution, the sample solution excites fluorescence, and an optical detection device is used to receive and analyze the fluorescence excited by the sample solution, thereby completing the detection of the sample solution. However, most of the optical detection parts of the existing PCR instruments detect a sample solution by a light source, and when there are a plurality of sample solution containing cavities in the PCR instrument, a plurality of light sources are required to detect the sample. The arrangement of a plurality of light sources can increase the production cost of the PCR instrument on one hand, and is not beneficial to the popularization of PCR in the market; a plurality of light sources can occupy the space in the PCR, and the volume of the PCR instrument can be correspondingly increased, so that the PCR instrument is not beneficial to placing or carrying.
The prior art discloses a PCR instrument, which comprises: the temperature control module comprises a base and a temperature control piece, wherein the base is provided with a detection channel, and the detection channel comprises a sample accommodating cavity, a first incident light channel communicated with the sample accommodating cavity and a first emergent light channel communicated with the sample accommodating cavity; the light source module comprises a light emitting source arranged opposite to the inlet of the first incident light channel; the photoelectric detection module comprises a photoelectric detector which is arranged opposite to the outlet of the first emergent light channel; the base comprises a first side and a second side which are opposite, the first side and the second side are arranged along a first direction, the detection channel is located between the first side and the second side, and the temperature control piece comprises a first temperature control piece attached to the first side and a second temperature control piece attached to the second side. According to the disclosed technology, through the design of the position and the connection relation of the temperature control module, the light source module and the photoelectric detection module, the size of the PCR instrument can be reduced, and the use scene of the PCR instrument is enlarged. However, each detection channel of the disclosed scheme corresponds to one light source, that is, one light source is used for detecting one sample solution, so that a plurality of light sources are still arranged in the device for detecting a plurality of sample solutions. However, the multiple light sources will increase the production cost of the PCR instrument on one hand, and are not favorable for the popularization of PCR in the market; a plurality of light sources can occupy the space in the PCR, and the volume of the PCR instrument can be correspondingly increased, so that the PCR instrument is not beneficial to placing or carrying.
SUMMERY OF THE UTILITY MODEL
The utility model discloses a overcome among the above-mentioned prior art PCR problem that a light source can only detect a sample, provide a PCR optical detection subassembly.
In order to solve the technical problem, the utility model discloses a technical scheme is: a PCR optical detection assembly comprises a light source for emitting a first light beam, a first beam splitter and an optical detection device; the first beam splitter receives the first light beam, reflects the first light beam to form a second light beam and transmits the second light beam to form a third light beam; the second light beam irradiates the first to-be-detected piece and forms a first excitation light path, the third light beam irradiates the second to-be-detected piece and forms a second excitation light path, and the optical detection device receives and processes light of the first excitation light path and the second excitation light path.
In this technical scheme, the first light beam that the light source sent can form two bundles of light that the direction is different after first beam splitter handles, and then can make the more waiting to measure of position of light detection that a isogenesis sent. In the technical scheme, a first light beam emitted by a light source is reflected and transmitted by a first beam splitter to form a second light beam and a third light beam which are different in direction. The second light beam irradiates the first piece to be tested and excites the first piece to be tested to generate a first excitation light path; the third light beam irradiates the second piece to be tested and excites the second piece to be tested to generate a second excitation light path; the optical detection device receives and processes the light of the first excitation light path and the light of the second excitation light path, and then the detection of the to-be-detected piece at two positions is completed.
Preferably, the light source further comprises a first light adjusting component for adjusting the path of the second light beam and a second light adjusting component for adjusting the path of the third light beam, wherein the first light adjusting component and the light source are positioned on the same side of the first beam splitter, and the second light adjusting component is positioned on the other side of the first beam splitter. In this way, the second light beam and the third light beam can be irradiated to a desired place according to the layout of the PCR instrument. Some devices such as temperature control are required to be arranged in the PCR instrument, so that the irradiation route of light in PCR needs to be designed according to the installation of other structures. The arrangement of the first light adjusting assembly and the second light adjusting assembly can enable the second light beam and the third light beam to change the routes according to actual conditions, and further enable the second light beam and the third light beam to irradiate to required positions. Meanwhile, the second light beam and the third light beam can be respectively subjected to light splitting processing according to the arrangement of the first light ray adjusting assembly and the second light ray adjusting assembly, and then the second light beam and the third light beam are irradiated to more positions.
Preferably, the second light adjusting assembly includes a first reflective mirror and a first dichroic mirror, the third light beam is reflected to the second to-be-detected element in sequence by the first reflective mirror and the first dichroic mirror, and the second excitation light path is transmitted to the optical detection device by the first dichroic mirror. In the technical scheme, according to the difference of light wavelengths, the first dichroic mirror only has a reflection effect on the light of the third light beam, and the first dichroic mirror only has a transmission effect on the second excitation light path. Therefore, light can be prevented from irradiating other positions, and light pollution of the light to the interior of the PCR instrument is reduced. Simultaneously, in this technical scheme, first light beam shines to first beam splitter with 45 jiaos on, and the contained angle of first reflector and first dichroic mirror and incident light also is 45, can make things convenient for the reflection or the transmission of light like this. Of course, some remaining angles are also within the scope of the present disclosure.
Preferably, the second light adjusting assembly further includes a second beam splitter and a second dichroic mirror, a portion of the third light beam is transmitted to the first reflective mirror through the second beam splitter, a portion of the third light beam is sequentially reflected to a third to-be-detected member through the second beam splitter and the second dichroic mirror to form a third excitation light path, and the third excitation light path is transmitted to the optical detection device through the second dichroic mirror. In this embodiment, the third light beam may be irradiated to the object to be measured at more positions. Meanwhile, through the arrangement of the second beam splitter and the second dichroic mirror, the transmitted or reflected light rays can be positioned in the same plane, and the transmitted or reflected light rays can also be positioned in different planes.
Preferably, the first light adjusting assembly includes a third dichroic mirror, the second light beam is reflected by the third dichroic mirror to the first object to be detected, and the first excitation light path is transmitted to the optical detection device through the third dichroic mirror. In the technical scheme, in the PCR instrument, the PCR optical detection principle mainly comprises that a light source irradiates a piece to be detected, then the piece to be detected generates an excitation light path, in the conventional PCR instrument, one light source irradiates one piece to be detected generally, and the optical detection component is mainly applied to the PCR instrument and realizes that one light source can irradiate a plurality of pieces to be detected. The first piece to be detected and the second piece to be detected are both vessels containing reactants and fluorescent substances, when the light source is turned on, the light emitted by the light source irradiates on a mixture of the reactants and the fluorescent substances, the fluorescent substances excite a light path after being irradiated, the optical detection device can judge the number of the pieces to be detected according to the intensity of the excitation light path, and then the detection of the pieces to be detected is completed. In this embodiment, the optical detection device may be a photodetector or some other device capable of analyzing the light emitted by the fluorescent material.
Preferably, the first light adjusting assembly further includes a third beam splitter, a part of the second light beam is reflected by the third beam splitter onto a fourth to-be-detected element and generates a fourth excitation light path, and the optical detection device receives and processes light of the fourth excitation light path; and part of the second light beam is transmitted to a third dichroic mirror through a third beam splitter.
Preferably, the first light adjusting assembly further includes a second reflective mirror and a fourth dichroic mirror, the second light beam reflected by the third reflective mirror is reflected to a fourth to-be-detected member in sequence by the second reflective mirror and the fourth dichroic mirror, and the fourth excitation light path is transmitted to the optical detection device by the fourth dichroic mirror.
Preferably, the first light beam is a parallel light beam. In this technical scheme, parallel light can make first light beam still keep for parallel state after refraction and reflection, makes things convenient for the irradiation of light beam to the piece that awaits measuring like this, and then makes it produce the excitation light path more easily.
Preferably, a filter is disposed between the first light ray adjusting assembly and the first beam splitter. In the technical scheme, the light with a certain wavelength is filtered through the filter, so that the light with the required wavelength is obtained, and the light pollution to the PCR instrument in the reflecting and transmitting processes of the light can be reduced.
Preferably, the first beam splitter is a neutral beam splitter. In this embodiment, the first beam splitter may split the first light beam into the second light beam and the third light beam having the same light intensity, and the second light beam and the third light beam are also protected by this embodiment.
Compared with the prior art, the utility model discloses the beneficial effect who produces is:
the utility model discloses in, the first light beam that the source sent can form the two bundles of light that the direction is different after first beam splitter handles, and then can make the more awaiting measuring pieces of position of light detection that a homology sent. In the technical scheme, a first light beam emitted by a light source is reflected and transmitted by a first beam splitter to form a second light beam and a third light beam which are different in direction. The second light beam irradiates the first piece to be tested and excites the first piece to be tested to generate a first excitation light path; the third light beam irradiates the second piece to be tested and excites the second piece to be tested to generate a second excitation light path; the optical detection device receives and processes the light of the first excitation light path and the light of the second excitation light path, and then the detection of the to-be-detected piece at two positions is completed. The utility model discloses a light source is accomplished simultaneously to the detection that waits to detect the sample of different positions, has reduced the setting of light source quantity in the PCR appearance, has reduced the manufacturing cost of PCR appearance, has saved the space that many light sources set up to occupy simultaneously, is favorable to the popularization of PCR in market.
Drawings
FIG. 1 is a schematic view of the PCR optical detection assembly of the present invention in an embodiment 1;
FIG. 2 is a schematic structural diagram of the PCR optical detection assembly of embodiment 2 of the present invention;
FIG. 3 is a schematic structural diagram of the PCR optical detection assembly of embodiment 3 of the present invention.
In the drawings: 1. a light source; 2. a first beam splitter; 3. a first to-be-tested member; 4. a second part to be tested; 5. an optical detection device; 6. a first light conditioning assembly; 7. a second light conditioning assembly; 8. a filter; 9. a third piece to be tested; 10. a fourth piece to be tested; 11. a first light beam; 31. a first excitation light path; 41. a second excitation light path; 61. a third dichroic mirror; 62. a third beam splitter; 63. a second reflective mirror; 64. a fourth dichroic mirror; 71. a first reflective mirror; 72. a first dichroic mirror; 73. a second beam splitter; 74. a second dichroic mirror; 91. a third excitation light path; 101. a fourth excitation optical path; 111. a second light beam; 112. and a third light beam.
Detailed Description
The drawings are for illustrative purposes only and are not to be construed as limiting the patent; for the purpose of better illustrating the present embodiments, certain elements of the drawings may be omitted, enlarged or reduced, and do not represent the size of an actual product; it will be understood by those skilled in the art that certain well-known structures in the drawings and descriptions thereof may be omitted. The positional relationships depicted in the drawings are for illustrative purposes only and should not be construed as limiting the present patent.
The same or similar reference numerals in the drawings of the embodiments of the present invention correspond to the same or similar parts; in the description of the present invention, it should be understood that if there are the terms "upper", "lower", "left", "right", "long", "short", etc. indicating the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, it is only for convenience of description and simplification of description, but it is not intended to indicate or imply that the device or element referred to must have a specific orientation, be constructed in a specific orientation, and be operated, and therefore the terms describing the positional relationship in the drawings are only for illustrative purposes and are not to be construed as limiting the present patent, and those skilled in the art will understand the specific meaning of the terms according to their specific circumstances.
The technical solution of the present invention is further described in detail by the following specific embodiments in combination with the accompanying drawings:
example 1
As shown in fig. 1, a PCR optical detection assembly comprises a light source 1 emitting a first light beam 11, a first beam splitter 2 and an optical detection device; the first beam splitter 2 receives the first light beam 11, reflects the first light beam to form a second light beam 111 and transmits the first light beam to form a third light beam 112; the second light beam irradiates the first to-be-detected piece and forms a first excitation light path, the third light beam irradiates the second to-be-detected piece and forms a second excitation light path, and the optical detection device receives and processes light of the first excitation light path and the second excitation light path.
In this embodiment, the first light beam 11 emitted by the light source 1 is processed by the first beam splitter 2 to form two light beams with different directions, so that one light beam emitted from the same source can detect more positions of the to-be-detected object. In the present embodiment, the first light beam 11 emitted from the light source 1 is reflected by the first beam splitter 2 and transmitted to form the second light beam 111 and the third light beam 112 with different directions. The second light beam 111 irradiates on the first object 3 and excites the first object 3 to generate a first excitation light path 31; the third light beam 112 irradiates on the second device under test 4 and excites the second device under test 4 to generate a second excitation light path 41; the optical detection device 5 receives and processes the received light from the first excitation light path 31 and the second excitation light path 41, and then completes the detection of the two position objects to be detected.
The light source device further comprises a first light adjusting component 6 for adjusting the route of the second light beam 111 and a second light adjusting component 7 for adjusting the route of the third light beam 112, wherein the first light adjusting component 6 and the light source 1 are positioned on the same side of the first beam splitter 2, and the second light adjusting component 7 is positioned on the other side of the first beam splitter 2. In this embodiment, the second beam 111 and the third beam 112 can be irradiated to a desired place according to the layout of the PCR instrument. Some devices such as temperature control are required to be arranged in the PCR instrument, so that the irradiation route of the light in the PCR instrument needs to be designed according to the installation of other structures. The first light adjusting assembly 6 and the second light adjusting assembly 7 are arranged to change the paths of the second light beam 111 and the third light beam 112 according to actual conditions, so that the second light beam 111 and the third light beam 112 are irradiated to desired positions. Meanwhile, the second light beam 111 and the third light beam 112 may be split according to the arrangement of the first light adjusting assembly 6 and the second light adjusting assembly 7, so that the second light beam 111 and the third light beam 112 irradiate to a plurality of positions.
The second light adjusting assembly 7 includes a first reflective mirror 71 and a first dichroic mirror 72, the third light beam 112 is sequentially reflected to the second to-be-detected element 4 through the first reflective mirror 71 and the first dichroic mirror 72, and the second excitation light path 41 is transmitted to the optical detection device 5 through the first dichroic mirror 72. In this embodiment, first dichroic mirror 72 only reflects light from third light beam 112 and first dichroic mirror 72 only transmits light from second excitation light path 41, depending on the wavelength of the light. Therefore, light can be prevented from irradiating other positions, and light pollution of the light to the interior of the PCR instrument is reduced. Meanwhile, in the present embodiment, the first light beam 11 is irradiated onto the first beam splitter 2 at an angle of 45 °, and the angles between the first reflective mirror 71 and the first dichroic mirror 72 and the incident light are also 45 °, so that the reflection or transmission of light can be facilitated. Of course, some of the remaining angles are within the scope of the present embodiment.
Wherein the first light beam 11 is a parallel light beam. In this embodiment, the parallel light beams can make the light beams of the first light beam 11 after refraction and reflection still keep in a parallel state, so as to facilitate the irradiation of the light beams on the object to be measured, and further make it easier to generate the excitation light path.
In addition, a filter 8 is arranged between the first light adjusting assembly 6 and the first beam splitter 2. In this embodiment, the light with a certain wavelength is filtered by the filter 8 to obtain the light with a desired wavelength, so that the light pollution to the PCR instrument during the reflection and transmission processes can be reduced.
Wherein, the first beam splitter 2 is a neutral beam splitter. In this embodiment, the first beam splitter 2 can split the first light beam 11 into the second light beam 111 and the third light beam 112 with equal beam intensity, and it is understood that the second light beam 111 and the third light beam 112 have different light intensity and are protected by this embodiment.
In addition, the first light adjusting assembly 6 includes a third dichroic mirror 61, the second light beam 111 is reflected to the first to-be-detected component 3 through the third dichroic mirror 61, and the first excitation light path 31 is transmitted to the optical detection device 5 through the third dichroic mirror 61. In this embodiment, the first to-be-detected piece 3 and the second to-be-detected piece 4 are both vessels containing reactants and fluorescent materials, when the light source 1 is turned on to irradiate the light emitted by the light source onto the mixture of the reactants and the fluorescent materials, the fluorescent materials are irradiated to excite the light path, and the optical detection device 5 can judge the number of the to-be-detected pieces according to the intensity of the excitation light path, so as to complete the detection of the to-be-detected pieces. In this embodiment, the optical detection device 5 may be a photodetector or some other device capable of analyzing the light emitted by the fluorescent material.
Example 2
Similar to embodiment 1, as shown in fig. 2, in this embodiment, a first light adjusting assembly 6 for adjusting the path of the second light beam 111 and a second light adjusting assembly 7 for adjusting the path of the third light beam 112 are included, the first light adjusting assembly 6 is located on the same side of the first beam splitter 2 as the light source 1, and the second light adjusting assembly 7 is located on the other side of the first beam splitter 2. In this embodiment, the second beam 111 and the third beam 112 can be irradiated to a desired place according to the layout of the PCR instrument. Some devices such as temperature control are required to be arranged in the PCR instrument, so that the irradiation route of the light in the PCR instrument needs to be designed according to the installation of other structures. The first light adjusting assembly 6 and the second light adjusting assembly 7 are arranged to change the paths of the second light beam 111 and the third light beam 112 according to actual conditions, so that the second light beam 111 and the third light beam 112 are irradiated to desired positions. Meanwhile, the second light beam 111 and the third light beam 112 may be split according to the arrangement of the first light adjusting assembly 6 and the second light adjusting assembly 7, so that the second light beam 111 and the third light beam 112 irradiate to a plurality of positions.
The second light adjusting assembly 7 includes a first reflective mirror 71 and a first dichroic mirror 72, the third light beam 112 is sequentially reflected to the second to-be-detected element 4 through the first reflective mirror 71 and the first dichroic mirror 72, and the second excitation light path 41 is transmitted to the optical detection device 5 through the first dichroic mirror 72. In this embodiment, first dichroic mirror 72 only reflects light from third light beam 112 and first dichroic mirror 72 only transmits light from second excitation light path 41, depending on the wavelength of the light. Therefore, light can be prevented from irradiating other positions, and light pollution of the light to the interior of the PCR instrument is reduced. Meanwhile, in the present embodiment, the first light beam 11 is irradiated onto the first beam splitter 2 at an angle of 45 °, and the angles between the first reflective mirror 71 and the first dichroic mirror 72 and the incident light are also 45 °, so that the reflection or transmission of light can be facilitated. Of course, some of the remaining angles are within the scope of the present embodiment.
In addition, the second light adjusting assembly 7 further includes a second beam splitter 73 and a second dichroic mirror 74, a portion of the third light beam 112 is transmitted to the first reflective mirror 71 through the second beam splitter 73, a portion of the third light beam 112 is sequentially reflected to the third object 9 to be detected through the second beam splitter 73 and the second dichroic mirror 74 to form a third excitation light path 91, and the third excitation light path 91 is transmitted to the optical detection device 5 through the second dichroic mirror 74. In this embodiment, the third light beam 112 can be irradiated onto the object to be measured at more positions. Meanwhile, the second beam splitter 73 and the second dichroic mirror 74 may be arranged to allow the transmitted or reflected light to be in the same plane, or allow the transmitted or reflected light not to be in the same plane.
Example 3
Similar to embodiment 2, as shown in fig. 3, in this embodiment, the first light adjusting assembly 6 includes a third dichroic mirror 61, the second light beam 111 is reflected onto the first device to be tested 3 through the third dichroic mirror 61, and the first excitation light path 31 is transmitted onto the optical detection apparatus 5 through the third dichroic mirror 61. In this embodiment, the first to-be-detected piece 3 and the second to-be-detected piece 4 are both vessels containing reactants and fluorescent materials, when the light source 1 is turned on to irradiate the light emitted by the light source onto the mixture of the reactants and the fluorescent materials, the fluorescent materials are irradiated to excite the light path, and the optical detection device 5 can judge the number of the to-be-detected pieces according to the intensity of the excitation light path, so as to complete the detection of the to-be-detected pieces. In this embodiment, the optical detection device 5 may be a photodetector or some other device capable of analyzing the light emitted by the fluorescent substance.
The first light adjusting assembly 6 further includes a third beam splitter 62, a part of the second light beam 111 is reflected to the fourth to-be-detected element 10 through the third beam splitter 62 and generates a fourth excitation light path 101, and the optical detection device 5 receives and processes light of the fourth excitation light path 101; part of the second light beam 111 is transmitted through the third beam splitter 62 onto the third dichroic mirror 61.
In addition, the first light adjusting assembly 6 further includes a second reflective mirror 63 and a fourth dichroic mirror 64, the second light beam 111 reflected by the third beam splitter 62 is sequentially reflected to the fourth object 10 through the second reflective mirror 63 and the fourth dichroic mirror 64, and the fourth excitation light path 101 is transmitted to the optical detection device 5 through the fourth dichroic mirror 64.
It is obvious that the above embodiments of the present invention are only examples for clearly illustrating the present invention, and are not limitations to the embodiments of the present invention. Other variations and modifications will be apparent to persons skilled in the art in light of the above description. And are neither required nor exhaustive of all embodiments. Any modification, equivalent replacement, and improvement made within the spirit and principle of the present invention should be included in the protection scope of the claims of the present invention.

Claims (10)

1. A PCR optical inspection assembly, characterized by: comprises a light source (1) emitting a first light beam (11), a first branch Shu Jing (2) and an optical detection device (5); the first branch Shu Jing (2) receives the first light beam (11), reflects the first light beam to form a second light beam (111) and transmits the first light beam to form a third light beam (112); the second light beam (111) irradiates a first piece to be tested (3) and forms a first excitation light path (31), the third light beam (112) irradiates a second piece to be tested (4) and forms a second excitation light path (41), and the optical detection device (5) receives and processes light of the first excitation light path (31) and the second excitation light path (41).
2. The PCR optical detection assembly of claim 1, wherein: the light source device also comprises a first light ray adjusting component (6) for adjusting the route of the second light beam (111) and a second light ray adjusting component (7) for adjusting the route of the third light beam (112), wherein the first light ray adjusting component (6) and the light source (1) are positioned on the same side of the first branch Shu Jing (2), and the second light ray adjusting component (7) is positioned on the other side of the first branch Shu Jing (2).
3. The PCR optical detection assembly of claim 2, wherein: the second light adjusting assembly (7) comprises a first reflective mirror (71) and a first dichroic mirror (72), the third light beam (112) is sequentially reflected to the second piece to be detected (4) through the first reflective mirror (71) and the first dichroic mirror (72), and the second excitation light path (41) is transmitted to the optical detection device (5) through the first dichroic mirror (72).
4. The PCR optical detection assembly of claim 3, wherein: the second light adjusting assembly (7) further comprises a second beam splitter (73) and a second dichroic mirror (74), a part of the third light beam (112) is transmitted to the first reflective mirror (71) through a second branch Shu Jing (73), a part of the third light beam (112) is sequentially reflected to a third piece to be detected (9) through a second branch Shu Jing (73) and the second dichroic mirror (74) to form a third excitation light path (91), and the third excitation light path (91) is transmitted to the optical detection device (5) through the second dichroic mirror (74).
5. The PCR optical detection assembly of claim 2, wherein: first light regulation subassembly (6) include third dichroic mirror (61), second light beam (111) reflect to first waiting measuring on piece (3) through third dichroic mirror (61), first excitation light path (31) are through on third dichroic mirror (61) transmission reaches optical detection device (5).
6. The PCR optical detection assembly of claim 5, wherein: the first light adjusting component (6) further comprises a third beam splitter (62), part of the second light beam (111) is reflected to a fourth to-be-detected piece (10) through a third branch Shu Jing (62) and generates a fourth excitation light path (101), and the optical detection device (5) receives and processes light of the fourth excitation light path (101); part of the second light beam (111) is transmitted through the third branch Shu Jing (62) onto the third dichroic mirror (61).
7. The PCR optical detection assembly of claim 6, wherein: the first light adjusting component (6) further comprises a second reflecting mirror (63) and a fourth dichroic mirror (64), a second light beam (111) reflected by the third light splitter Shu Jing (62) is reflected to a fourth piece to be detected (10) through the second reflecting mirror (63) and the fourth dichroic mirror (64) in sequence, and the fourth excitation light path (101) is transmitted to the optical detection device (5) through the fourth dichroic mirror (64).
8. The PCR optical detection assembly of claim 1, wherein: the first light beam (11) is a parallel light beam.
9. The PCR optical detection assembly of claim 2, wherein: a filter (8) is arranged between the first light ray adjusting component (6) and the first branch Shu Jing (2).
10. The PCR optical detection assembly of claim 1, wherein: the first branch Shu Jing (2) is a neutral beam splitter.
CN202221359916.2U 2022-05-31 2022-05-31 PCR optical detection assembly Active CN218382403U (en)

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Application Number Priority Date Filing Date Title
CN202221359916.2U CN218382403U (en) 2022-05-31 2022-05-31 PCR optical detection assembly

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Application Number Priority Date Filing Date Title
CN202221359916.2U CN218382403U (en) 2022-05-31 2022-05-31 PCR optical detection assembly

Publications (1)

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CN218382403U true CN218382403U (en) 2023-01-24

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