CN218232349U - Device for aseptically culturing turnip duckweed with multiple groups of comparison functions - Google Patents

Abstract

The utility model discloses a device with multiple groups of comparison functions for aseptically culturing turnip duckweed, which comprises a base, wherein a plurality of fan-shaped and transparent gradient comparison culture tanks are radially distributed in the = base; a top cover made of transparent material is respectively arranged at the top of each gradient contrast culture tank; the side wall of each gradient control culture tank is provided with a first air hole, and each first air hole is covered with a first breathable film. The utility model discloses the advantage does: 1. the whole body is made of transparent materials, the growth condition of algae can be observed without opening the cover, and the problem of mixed bacteria pollution caused by opening the cover can be avoided; 2. through the arrangement of the air holes and the application of the air-permeable film on the air holes, air exchange is realized, meanwhile, the entry of mixed bacteria into the groove is avoided, and sterile culture is realized; 3. the aim of carrying out multi-group gradient contrast culture observation under different nutrient environments is fulfilled by a plurality of separating grooves.

Description

Device for Aseptic Cultivation of Wudao with Multiple Control Functions

technical field

The utility model relates to the technical field of aseptic culture, in particular to a device for aseptic culture of Wuping with multi-group control functions.

Background technique

Single-celled algae such as Wuping are extremely small, and their morphological structure is difficult to observe. It is difficult to directly identify the family, genus and species based on their morphological characteristics with the naked eye, and must be observed with a microscope. And in the course of the experiment, it was found that the morphological structure of Wuping would change under different nutritional conditions. But at the same time, single-celled algae such as turnips are too small, and they are easily dehydrated to death in a short period of time when they are focused by the microscope light source after leaving the aquatic environment, making it difficult to record and analyze their morphological structure.

At present, when performing microscopic observation, it is necessary to take the aseptically cultivated Wuping out of the tissue culture bottle and place it on the stage of the microscope. It is necessary to frequently adjust the microscope and algae from multiple angles, and finally take pictures and record them.

There are many limitations in this method: the first is that the tissue culture bottle needs to be opened during observation, and a small amount of algae is taken out for observation, so the remaining algae in the bottle will lose the aseptic culture environment due to the opening of the culture bottle, causing contamination by bacteria; One is that the observation is often interrupted due to the dehydration and death of the algae, so that a comprehensive algae morphological structure change process cannot be obtained; the third is that if the algae morphological structure under different nutritional environments is observed, then the observation results under different variables at the same time cannot be accurately obtained. , and the observation content is complicated, time-consuming, and increases the cost of the experiment.

Contents of the invention

The purpose of the utility model is to provide a device for aseptic culture of Wuping with multi-group control function, which can not only realize aseptic culture, but also realize multi-group gradient control culture observation under different nutritional environments.

In order to achieve the above object, the utility model can take the following technical solutions:

The device for aseptic cultivation of Wuping with multi-group control functions described in the utility model includes a base, and a plurality of fan-shaped and transparent gradient control culture tanks are radially distributed in the base, and the gradient control culture The number of tanks can be determined according to market demand, and gradient experiments can be carried out or different nutritional environments can be given to realize the simultaneous comparison and observation of the growth of algae under multiple groups of different conditions in the same device; at the top of each gradient control culture tank The top cover of transparent material is respectively set, and the top cover and the gradient control culture tank are both made of transparent material. The growth of algae can be observed without opening the cover, which can avoid the problem of miscellaneous bacteria pollution caused by opening the cover; The side walls of the tank are provided with first air holes, and each of the first air holes is covered with a first air-permeable film, and the first air-holes and the first air-permeable film can ensure that the gradient control culture tank is in normal ventilation. While ensuring a sterile environment.

Further, the base is circular, and a transparent blank control culture tank is provided at the central point of the base, and a circular top cover of transparent material is provided on the top of the blank control culture tank. The tank wall of the culture tank is provided with a second air hole, covered with a second air-permeable film on the second air hole, and the blank control culture tank is arranged in the middle, which is convenient to distinguish the blank group and the control group; the groove of the blank control culture tank There is a breathable ring groove between the wall and the inner wall of the gradient control culture tank, which is convenient for the normal ventilation of the blank group.

The utility model advantage is:

1. The whole is made of transparent material, and the growth of algae can be observed without opening the cover, which can avoid the problem of bacteria pollution caused by opening the cover;

2. By setting air holes and pasting air-permeable membranes on the air holes, it is possible to achieve ventilation while preventing bacteria from entering the tank to achieve aseptic culture;

3. Through multiple separation tanks, the purpose of multi-group gradient control culture observation under different nutritional environments is realized.

Using this device, there is no need to open the cover and take it out for observation, which avoids the problem of the experimental results being affected by the morphological changes of Wuping due to dehydration during the observation process, which leads to the problem that the experimental results are not rigorous enough; the purpose of continuously recording its dynamic growth process is achieved, and finally the shape of Wuping is recorded. Complete records such as size, color change, and budding conditions are used as experimental material support.

Description of drawings

Fig. 1 is the front view of the utility model.

Fig. 2 is a top view of the utility model.

Fig. 3 is a perspective view of the utility model.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. Example. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present utility model.

It should be noted that all directional indications (such as up, down, left, right, front, back...) in the embodiments of the present utility model are only used to explain the relationship between the components in a certain posture (as shown in the accompanying drawings). If the specific posture changes, the directional indication will also change accordingly.

As shown in Figures 1-3, the device for aseptically cultivating Wuping with multi-group control functions described in the utility model includes a circular base, and a plurality of them are evenly distributed radially in the base (shown in the figure) 8) fan-shaped and transparent gradient control culture tanks 10, the number of gradient control culture tanks 10 can be determined according to market demand, gradient experiments can be performed or different nutritional environments can be provided, and multiple groups of different nutritional environments can be compared and observed simultaneously in the same device. The growth situation of algae under condition; Be provided with top cover 11 respectively at the top of each gradient contrast culture tank 10, top cover 11 and gradient contrast culture tank 10 are all transparent materials, can observe the growth situation of algae without opening cover, can Avoid the problem of miscellaneous bacteria pollution caused by opening the cover; the outer side wall of each gradient control culture tank 10 is provided with a first air hole 12, and each first air hole 12 is covered with a first air-permeable film 13, The first air hole 12 and the first air-permeable film 13 can ensure that the gradient control culture tank 10 ensures a sterile environment while normal ventilation; a transparent blank control culture tank 20 is arranged at the central point position in the base, and in the blank control The top of the culture tank 20 is provided with a circular top cover 21, and the tank wall of the blank control culture tank 20 is provided with a second air hole, and the second air hole is covered with a second air-permeable film, and the blank control culture tank 20 is arranged in the middle. , to facilitate the distinction between the blank group and the control group; between the tank wall of the blank control culture tank 20 and the inner wall of the gradient control culture tank 10, there is a vent ring groove 30, which is convenient for the normal ventilation of the blank group.

Use this device to carry out the aseptic culture test of single-cell algae. Because the whole is made of transparent material, you can observe the growth of algae without opening the cover, which can avoid the problem of bacterial contamination caused by opening the cover; ventilate through the opened vent holes , prevent bacteria from entering the tank through the attached breathable membrane, and realize aseptic culture; realize the purpose of multi-group gradient control culture observation under different nutritional environments through multiple separate tanks.

Claims (2)

1. a kind of device that is used for the aseptic culture Wuping that has many groups of control functions, comprises base, it is characterized in that, in described base, be radially distributed with a plurality of fan-shaped and transparent gradient control culture tanks, in The top of each gradient control culture tank is respectively provided with a top cover of transparent material, and the side wall of each gradient control culture tank is provided with a first air hole, and each of the first air hole is covered with The first breathable membrane. 2. The device for aseptically cultivating Wuping with multi-group control functions according to claim 1, characterized in that: the base is circular, and a transparent glass is provided at the central point of the base. The top of the blank control culture tank is provided with a circular top cover of transparent material, and the wall of the blank control culture tank is provided with a second air hole, and the second air hole is covered with the first air hole. Second, there is a breathable membrane; Wherein, there is a gas-permeable ring groove between the tank wall of the blank control culture tank and the inner wall of the gradient control culture tank.

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