CN206529483U - A kind of plant callus culture vessel - Google Patents

Abstract

The utility model relates to a plant callus culture vessel, which comprises a culture vessel and an upper cover. The culture vessel and the upper cover are seamlessly connected, and a detachable and recyclable connection belt is arranged between the two for fixing. , by setting mutually symmetrical protruding points between the culture dish and the upper cover, it is used for the snap-type fixed connection between the connection belt pair. The middle position of the upper surface of the upper cover is provided with a cylindrical ventilation cavity layer, which is provided with a cover with small air holes and a bottom with a breathable film integrated with the upper cover of the petri dish, which can be opened and contains small air holes. The cover part of the air hole replaces the absorbent cotton or the air-permeable membrane in the cavity layer. The utility model can effectively solve the shortcomings of the unsteady cover and bottom of the traditional culture dish. The processing is simple, environmentally friendly and effective, and it is convenient to operate. Conducive to the cultivation of plant callus.

Description

A plant callus culture vessel

technical field

The utility model relates to a plant tissue culture vessel, in particular to a plant callus culture vessel.

Background technique

At present, the materials of petri dishes are basically divided into two types: plastic and glass, generally consisting of a flat disc-shaped bottom and a cover, which can be used for plant materials, microbial culture, animal cell adherent culture and laboratory inoculation, Scribe, isolate bacteria, etc. Most of the existing culture vessels used for plant callus culture have unstable lids and bottoms and a certain risk of bacterial contamination. In order to solve these problems, some external materials are needed to stabilize the operation. cumbersome. On the other hand, the existing culture vessels still have the disadvantage of poor air permeability, which cannot meet the requirements for air circulation and moisture in the plant callus culture process, and are not conducive to plant callus culture.

Contents of the invention

Aiming at the defects in the above-mentioned prior art, the utility model provides a plant callus culture vessel whose cover and bottom are not easy to loose, easy to store, easy to process and environmentally friendly, and can effectively isolate bacteria from damaging plant tissues. Potential threats, ensure the circulation of air and water, and can significantly improve the efficiency of experimental operations, which is conducive to plant callus culture.

In order to solve the above technical problems, the utility model is solved through the following technical solutions:

A plant callus culture vessel, comprising a culture dish and an upper cover, the culture dish and the upper cover are seamlessly connected, and a detachable and recyclable connection belt is set between the culture dish and the upper cover to fix, Two mutually symmetrical raised points are arranged on the outer wall of the culture dish, and corresponding raised points are arranged on the edge of the upper surface of the upper cover at the same time, and the raised points are used for the snap-type fixed connection of the culture dish and the upper cover by the connecting belt.

As a preference, a cylindrical ventilation cavity layer is provided in the middle of the upper surface of the upper cover, and the cylindrical ventilation cavity layer includes a cover with 2-4 small ventilation holes and an upper connection with the petri dish. The bottom of the air-permeable membrane is integrated with the injection molding of the cover. At the same time, absorbent cotton or air-permeable membrane can be placed in the cavity layer, which can ensure sufficient light, isolate bacteria, and ensure the circulation of air and moisture.

Preferably, both the culture dish and the upper cover are made of transparent materials.

As preferably, the cover part containing the small vent holes of the cylindrical ventilation cavity layer and the bottom part provided with the gas-permeable membrane integrated with the upper cover injection molding of the culture dish are connected by threads, and the cap part containing the small vent holes is connected by thread. The cover is provided with a flange that is easy to rotate, so that it can be easily unscrewed to replace the absorbent cotton or air-permeable membrane placed in it; or use a cover with a certain elasticity on the material that contains small air holes. Set the concave point, connect the cover and the bottom of the cylindrical ventilation cavity layer in a way similar to snap buttons, and the diameter of the cover of the cylindrical ventilation cavity layer needs to be slightly larger than the diameter of the bottom, which is conducive to fixing and also helps Interacting with the flange provided on the cover, it is convenient to open and replace the absorbent cotton or breathable membrane placed in it.

As a preference, the overall occupancy of the cylindrical ventilation cavity layer needs to be smaller than the diameter of the upper cover, and its height is almost flush with the upper surface of the upper cover of the culture dish, and the overall length of the inner surface of the upper cover is 6-9mm. The height of the dish should be higher than the upper cover, which is conducive to the cultivation of plant callus and is convenient for observing the changes in the process of plant callus culture.

Preferably, the culture dish and the upper cover are connected through a seamless joint, and a detachable and recyclable connection belt is arranged between the two to fix them. This can not only effectively solve the shortcomings of the unstable lid and bottom of the traditional culture dish, but also facilitate the operation, and at the same time, it can isolate the bacteria from invading the plant tissue, and can also play a certain role in environmental protection.

Due to the adoption of the above technical solutions, the utility model has remarkable technical effects: the utility model has a simple structure, is convenient to use, has universal materials, is transparent, is convenient for observation, and is beneficial to sunlight irradiation at the same time. The upper cover of the petri dish is equipped with a cylindrical ventilation cavity layer device, which is conducive to the circulation of air and water, and can effectively prevent bacteria from entering and invading plant tissues, and is also conducive to adjusting the temperature and humidity of the petri dish. The seamless connection between the petri dish and the upper cover and the setting of the detachable and recyclable connecting belt are convenient for operation, and at the same time, it effectively solves the shortcomings of the traditional petri dish’s unstable cover and bottom, and plays a role in isolating bacteria and protecting the environment. .

Description of drawings

Fig. 1 is the structural representation of the utility model.

Fig. 2 is a perspective view of the first embodiment of the upper cover of the present invention.

Fig. 3 is a structural schematic diagram of the first specific embodiment of the structure of the cylindrical ventilation cavity layer in the upper cover of the present invention.

Fig. 4 is a perspective view of a second embodiment of the upper cover of the present invention.

Fig. 5 is a structural schematic diagram of a second specific embodiment of the structure of the cylindrical ventilation cavity layer in the upper cover of the present invention.

The parts indicated by the numerals in the above drawings are as follows: 1-petri dish, 2-top cover, 3-cylindrical ventilation cavity layer, 31-cylindrical ventilation cavity layer bottom, 32-cylindrical ventilation cavity The cover part of the cavity layer, 33-flange, 34-small air hole, 35-thread, 36-inner concave point, 4-connecting belt.

detailed description

In order to enable those skilled in the art to better understand the solution of the utility model, the utility model will be further described in detail below in conjunction with the accompanying drawings and specific embodiments:

Embodiment one:

A plant callus culture vessel, comprising a culture vessel 1 and an upper cover 2, both of which are made of transparent materials. The petri dish 1 is seamlessly connected with the upper cover 2, and a detachable and recyclable connection belt 4 is set between the petri dish 1 and the upper cover 2 to fix it. Two mutually symmetrical The protruding point is provided with a corresponding protruding point at the edge of the upper surface of the upper cover 2 at the same time, and the protruding point is used for the connection belt 4 to carry out snap-type fixed connection of the culture dish 1 and the upper cover 2 . At the same time, a cylindrical ventilation cavity layer 3 is provided at the middle position on the upper surface of the upper cover 2, and the cylindrical ventilation cavity layer 3 includes a cover portion 32 with 2-4 small ventilation holes 34 and a connection with the culture dish. The bottom 31 of the upper cover 2 is injection-molded and provided with a breathable membrane. At the same time, absorbent cotton or a breathable membrane can be placed in the cavity layer, so as to ensure sufficient light, isolate bacteria, and ensure the circulation of air and moisture.

The lid portion 32 containing the small air holes 34 of the cylindrical ventilation cavity layer 3 is connected by a screw thread 35 between the bottom 31 that is injection-molded with the upper cover 2 of the culture dish and is provided with a gas-permeable membrane, and contains small holes in it. The cover portion 32 of the vent hole 34 is provided with a flange 33 which is convenient for rotation, so that it can be easily unscrewed to replace the absorbent cotton or air-permeable membrane placed therein.

The cylindrical ventilating cavity layer 3 needs to be smaller than the diameter of the upper cover 2 as a whole, and its height is almost flush with the upper surface of the upper cover 2 of the culture dish, and the overall length of the inner surface of the upper cover 2 is 6-9 mm. The height of the petri dish 1 is higher than its upper cover 2, which is conducive to the cultivation of plant callus and is convenient for observing the changes in the process of plant callus culture.

The culture dish 1 and the upper cover 2 are connected through a seamless joint, and a detachable and recyclable connection belt 4 is arranged between the two to fix it. This can not only effectively solve the shortcomings of the unstable lid and bottom of the traditional culture dish, but also facilitate the operation, and at the same time, it can isolate the bacteria from invading the plant tissue, and can also play a certain role in environmental protection.

Embodiment two:

A plant callus culture vessel, compared with Example 1, except that the structure of the cylindrical ventilating cavity layer 3 in the upper cover 2 of the culture vessel 1 or the selection of materials are partially changed, the rest are the same as those in Example 1. One said the same. The change is that the connection mode between the cover portion 32 containing the small air holes 34 of the cylindrical ventilation cavity layer 3 and the bottom 31 which is injection-molded with the upper cover 2 of the culture dish and provided with a gas-permeable membrane is different, wherein The material of the selected cover 34 needs to have a certain degree of elasticity. The cover 32 and the bottom 31 are connected by the elasticity of the material itself in a manner similar to snap buttons, and an inner concave point 36 is set at about half the height of the cover 32 as a fixed point. And the diameter of its cover 32 needs to be slightly larger than the diameter of the bottom 31 of the cylindrical ventilation cavity layer, which is conducive to fixing, and also helps to interact with the flange 33 provided on the cover 32, so as to be convenient to open and replace the placed in it. Absorbent cotton or breathable membrane.

The above descriptions are only preferred embodiments of the present invention, and all equivalent changes and modifications made according to the patent scope of the present invention shall fall within the scope of the patent of the present invention.

Claims (3)

1. A plant callus culture vessel, comprising a culture dish (1) and a loam cake (2), all adopting a transparent material; it is characterized in that: the culture dish (1) is seamlessly connected with the loam cake (2) , and a detachable and recyclable connection belt (4) is set between the culture dish (1) and the upper cover (2) to fix it, and two mutually symmetrical convex points are arranged on the outer wall of the culture dish (1), and the upper cover (2) Corresponding protruding points are provided at the edge of the upper surface at the same time, and the protruding points are used for the fixed connection between the connection belt (4) and the culture dish (1) and the upper cover (2). 2. A kind of plant callus culture vessel according to claim 1, characterized in that: the middle position of the upper surface of the upper cover (2) is provided with a cylindrical ventilation cavity layer (3), the The cylindrical ventilation cavity layer (3) comprises a cover (32) with 2-4 small ventilation holes (34) and a bottom (31) provided with a gas-permeable membrane integrally injected with the upper cover (2) of the culture dish. ), and absorbent cotton or breathable membrane can be placed in the cavity layer at the same time. 3. a kind of plant callus culture vessel according to claim 2, is characterized in that: the lid portion (32) that contains small air hole (34) of described cylindrical ventilating cavity layer (3) and with The upper cover (2) of the culture dish is injection-molded and the bottom (31) provided with a gas-permeable membrane is connected by a screw thread (35); or by utilizing the elasticity of the material itself, a concave is set at about half the height of the cover (32) The point (36) is fixedly connected by a method similar to a snap button, and the diameter of its cylindrical ventilation cavity layer cover (32) needs to be slightly larger than the diameter of the bottom (31), and it contains small air holes (34) A flange (33) for easy rotation is arranged on the cover part (32).