CN206872850U - A kind of animal cell culture device - Google Patents

Abstract

The utility model discloses an animal cell culture device, which comprises a dish body and a dish cover. The dish body includes a bottom, an annular side wall and an annular bottom wall. The upper surface of the bottom and the inner surface of the annular side wall form a Cell culture chamber; the dish cover includes a top and an annular side, and the entire dish body is located in a constant temperature and humidity constant oxygen regulator; the constant temperature and humidity constant oxygen regulator includes a cooling and heating device, a humidifying device and a fresh air ventilation device . The cell culture chamber has an arc-shaped bottom wall, and a discharge pipe is arranged in the middle of the bottom end of the arc-shaped bottom wall, and the discharge pipe communicates with the inside of the cell culture chamber, and a discharge pipe is installed on the discharge pipe. A regulator, the regulator is used to adjust the opening of the discharge pipe. The cultured animal cells are facilitated to be discharged by being provided with an arc-shaped bottom wall and a discharge pipe. A pipette is installed on the dish cover, and the pipette communicates with the inside of the cell culture chamber, and a cover is installed on the pipette, and a cover handle is installed on the cover.

Description

An animal cell culture device

technical field

The utility model relates to the technical field of animal cell culture, in particular to an animal cell culture device.

Background technique

An incubator is a laboratory vessel used for cell culture, generally consisting of a flat disc-shaped bottom and a cover. The incubator is basically divided into two types according to the material, one is plastic and the other is glass. Glass can be used for plant materials, microbial culture and animal cell adherent culture. The plastic ones may be made of polyethylene materials, and there are disposable and multiple-use ones, which are suitable for laboratory inoculation, streaking, and isolation of bacteria, and can be used for the cultivation of plant materials.

Animal cells need a sterile environment during the culture process, and whether they are in vivo cells or in vitro cells, they need to survive at a constant temperature. Generally, the most suitable growth temperature is 37°C, and the temperature change should not exceed ±0.5°C. This is mainly because Determined by the optimal temperature required by enzymes and proteins. When the temperature is too low, the cells grow slowly or even do not grow. When the temperature is too high, the cells will die. The current animal cell culture device can only adjust the temperature, but cannot adjust the humidity, oxygen content, and air cleanliness required for cell culture. At the same time, the existing cell culture device cannot effectively discharge the cells in time after the culture is completed.

Utility model content

In order to overcome the deficiencies in the prior art, the utility model provides an animal cell culture device.

The utility model is realized through the following technical solutions:

An animal cell culture device, comprising a dish body for placing culture medium and a dish cover made of transparent high-temperature-resistant material, the dish body includes a bottom, an annular side wall extending upward from the upper surface of the bottom, An annular bottom wall extending outward from the lower surface of the bottom, a cell culture chamber is formed between the upper surface of the bottom and the inner surface of the annular side wall; The annular side portion extending outwards is provided with a rotating handle on the top of the vessel cover. The whole dish body is located in the constant temperature, constant humidity and constant oxygen regulator; the constant temperature and constant humidity constant oxygen regulator includes a cooling and heating device, a humidifying device and a fresh air ventilation device. The cell culture chamber has an arc-shaped bottom wall, and a discharge pipe is arranged in the middle of the bottom end of the arc-shaped bottom wall, and the discharge pipe communicates with the inside of the cell culture chamber, and a discharge pipe is installed on the discharge pipe. A regulator, the regulator is used to adjust the opening of the discharge pipe. The cultured animal cells are facilitated to be discharged by being provided with an arc-shaped bottom wall and a discharge pipe. A pipette is installed on the dish cover, and the pipette communicates with the inside of the cell culture chamber, and a cover is installed on the pipette, and a cover handle is installed on the cover. A sealing groove A is processed on the outer surface of the bottom of the dish body, a sealing ring A is installed in the sealing groove A, a sealing groove B is processed on the outer surface of the annular side wall of the dish body, and a sealing groove B is processed in the sealing groove A. A sealing ring B is installed in B.

Compared with the existing technology, the beneficial effect of the utility model is: the utility model is equipped with a constant temperature, humidity and constant oxygen regulator, which can realize the control of the air temperature, humidity and oxygen content in the animal cell culture environment, thereby Create a good environment for animal cell culture; through the arc-shaped bottom wall and discharge pipe, it is convenient to discharge the cultured animal cells; through the pipette, the inoculation loop or pipette tip passes through the pipette Inoculate or add samples to the surface of the culture medium in the cell culture chamber; by setting the sealing ring A and the sealing ring B, the sealing performance between the dish body and the dish cover can be improved, and the culture dish and the culture medium can be prevented during transportation. Cross-contamination of microorganisms outside the dish.

Description of drawings

Fig. 1 is the structural representation of the utility model.

detailed description

In order to make the purpose, technical solution and advantages of the utility model clearer, the utility model will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the utility model, and are not intended to limit the utility model.

Please refer to Fig. 1, described a kind of animal cell cultivator, comprises the vessel body 1 that is used to place culture medium and the vessel cover 2 that is made by transparent high temperature resistant material, and described vessel body 1 comprises bottom 11, from described An annular side wall 12 extending upward from the upper surface of the bottom 11, and an annular bottom wall 13 extending outward from the lower surface of the bottom 11, forming a gap between the upper surface of the bottom 11 and the inner surface of the annular side wall 12. Cell culture chamber 3 ; the dish cover 2 includes a top 21 , and an annular side portion 22 extending outward from the lower surface of the top 21 , and a rotating handle 23 is provided on the top 21 of the dish cover 2 .

The entire dish body 1 is located in the constant temperature and humidity constant oxygen regulator 4; the constant temperature and humidity constant oxygen regulator 4 includes a cooling and heating device, a humidifying device and a fresh air ventilation device. It is worth noting that the working principle of the constant temperature, constant humidity and constant oxygen regulator 4 in the utility model can refer to the utility model patent with the Chinese application number 201120293167.3, and the patent name is "constant temperature and humidity constant oxygen air conditioner".

The cell culture chamber 3 has an arc-shaped bottom wall 31, and a discharge pipe 32 is arranged in the middle of the bottom end of the arc-shaped bottom wall 31, and the discharge pipe 32 communicates with the inside of the cell culture chamber 3, and is connected to the inside of the cell culture chamber 3. A regulator 33 is installed on the discharge pipe 32 , and the regulator 33 is used to adjust the opening degree of the discharge pipe 32 . The arc-shaped bottom wall 31 and the discharge pipe 32 are provided to facilitate the discharge of the cultured animal cells.

A pipette 5 is installed on the dish cover 2, and the pipette 5 communicates with the inside of the cell culture chamber 3, and a cover 51 is installed on the pipette 5, and a cover is installed on the cover 51. handle 52. The inoculation loop or pipette tip is used to inoculate or add samples to the surface of the culture medium in the cell culture chamber 3 through the pipette 5 .

A seal groove A is processed on the bottom 11 outer surface of the dish body 1, a seal ring A14 is installed in the seal groove A, and a seal groove B is processed on the annular side wall 12 outer surface of the dish body 1. A sealing ring B15 is installed in the sealing groove B. By providing the sealing ring A14 and the sealing ring B15, the sealing performance between the dish body 1 and the dish cover 2 can be improved, and cross-contamination of microorganisms inside and outside the culture dish can be prevented during transportation.

The above are only preferred embodiments of the present utility model, and are not intended to limit the present utility model. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present utility model shall be included in the utility model. within the scope of protection.

Claims (4)

1. a kind of animal cell culture device, including for placing the ware body (1) of culture medium and made of transparent high temperature resistant material Ware lid (2), the ware body (1) include bottom (11), the annular sidewall of setting are upwardly extended from the upper surface of the bottom (11) (12), the following table from the bottom (11) extends outwardly the annular bottom wall (13) of setting, the upper surface of the bottom (11) with Cell culture chamber (3) is formed between the inner surface of annular sidewall (12)；The ware lid (2) includes top (21), from the top (21) following table extends outwardly the annular side portions (22) of setting；It is characterized in that：Whole ware body (1) is located at constant temperature and humidity perseverance oxygen In adjuster (4)；The cell culture chamber (3) has arc bottom wall (31), in the bottom interposition of the arc bottom wall (31) Install and be equipped with discharge nozzle (32), and discharge nozzle (32) communicates with cell culture chamber (3) inside, and pacify on the discharge nozzle (32) Equipped with adjuster (33), the adjuster (33) is used for the opening degree for adjusting discharge nozzle (32)；It is provided with the ware lid (2) Pipette (5), and pipette (5) communicates with cell culture chamber (3) inside, and lid (51) is installed on pipette (5), Cap handle (52) is installed on the lid (51).

A kind of 2. animal cell culture device according to claim 1, it is characterised in that：In the bottom of the ware body (1) (11) outer surface is machined with seal groove A, and sealing ring A (14) is provided with the seal groove A, in the annular of the ware body (1) Side wall (12) outer surface is machined with seal groove B, and sealing ring B (15) is provided with the seal groove B.

A kind of 3. animal cell culture device according to claim 2, it is characterised in that：At the top of the ware lid (2) (21) rotating handle (23) is provided with.

A kind of 4. animal cell culture device according to claim 1, it is characterised in that：The constant temperature and humidity perseverance oxygen adjuster (4) refrigerating and heating combined equipment, humidification device and fresh air-changing device are included.