CN216837977U

CN216837977U - Constant temperature device for cell fusion

Info

Publication number: CN216837977U
Application number: CN202123145902.9U
Authority: CN
Inventors: 王战争; 刘晓磊; 张会刚; 张慧丽; 张彩霞
Original assignee: Zhengzhou Saitukang Biotechnology Co ltd
Current assignee: Zhengzhou Saitukang Biotechnology Co ltd
Priority date: 2021-12-15
Filing date: 2021-12-15
Publication date: 2022-06-28
Anticipated expiration: 2031-12-15

Abstract

The utility model discloses a constant temperature device for cell fusion, which comprises a constant temperature box body, wherein the lower part of the constant temperature box body is provided with a separation plate, and the separation plate divides the constant temperature box body into a heating area positioned above and a driving area positioned below; a heating medium is placed in the heating zone; a motor is arranged in the driving area, an output shaft of the motor is connected with a rotating rod, and the rotating rod is rotatably connected with the isolating plate and extends into the heating area; a first supporting disc and a second supporting disc are sequentially arranged on the part, located in the heating area, of the rotating rod from top to bottom; the first support disc is symmetrically provided with at least two mounting holes for placing centrifuge tubes, the second support disc is provided with conical through holes for supporting the lower parts of the centrifuge tubes, and the conical through holes and the mounting holes are arranged in a one-to-one correspondence manner; a liquid inlet is formed in the first supporting disc; and an annular air bag used for tightly propping the centrifugal tube is arranged on the inner wall of the mounting hole.

Description

Constant temperature device for cell fusion

Technical Field

The utility model belongs to the technical field of cell culture, concretely relates to a constant temperature equipment for cell fusion.

Background

Cell fusion refers to fusion of two cells or protoplasts of different genotypes under spontaneous or artificial induction to differentiate and regenerate the cells or protoplasts to form a hybrid cell, so as to overcome the barrier of incompatibility, combine the excellent properties of various plants more widely and culture an ideal variety. In the existing cell fusion, a centrifuge tube of about 50ml is generally required to be placed in a water bath kettle, and a constant temperature environment of 37 ℃ is provided for the centrifuge tube. Above-mentioned process is because the stirring rod need stretch into in the centrifuging tube, consequently when withdrawing from the centrifuging tube with the stirring rod, the material of adhesion can drip outside the centrifuging tube on the stirring rod, and because the existence of stirring rod during the stirring, the centrifuging tube need be in open state, therefore this type of constant temperature equipment has great drawback.

SUMMERY OF THE UTILITY MODEL

In order to solve the technical problem, the utility model discloses a following technical scheme:

the utility model discloses a constant temperature equipment for cell fusion, including the thermostated container body, the lower part of the thermostated container body is provided with the division board, the division board divides the thermostated container body into the heating zone that is located the top and with the drive zone that is located the below; a heating medium is placed in the heating zone; a motor is arranged in the driving area, an output shaft of the motor is connected with a rotating rod, and the rotating rod is rotatably connected with the isolating plate and extends into the heating area; a first supporting disc and a second supporting disc are sequentially arranged on the part, located in the heating area, of the rotating rod from top to bottom; the first support disc is symmetrically provided with at least two mounting holes for placing centrifuge tubes, the second support disc is provided with conical through holes for supporting the lower parts of the centrifuge tubes, and the conical through holes and the mounting holes are arranged in a one-to-one correspondence manner; a liquid inlet is formed in the first supporting disc; and an annular air bag used for tightly propping the centrifugal tube is arranged on the inner wall of the mounting hole.

Furthermore, an annular groove is formed in the inner wall of the incubator body, and the edge of the first support disc is clamped in the annular groove; a protective door is hinged on the liquid inlet; the diameter of the second supporting disk is smaller than the inner diameter of the thermostat body, and a plurality of water passing holes for water permeation are further formed in the second supporting disk.

Furthermore, an air charging and discharging device is arranged on the upper surface of the annular air bag; the inflation and deflation device comprises an air inlet pipe communicated with the inside of the annular air bag, a sleeve connected to the upper end of the air inlet pipe and an opening and closing assembly positioned in the sleeve and used for opening and closing the air inlet pipe; the lower end of the sleeve extends into the air inlet pipe, and the outer wall of the sleeve is connected with the air inlet pipe in a sealing manner; the air inlet pipe and the sleeve are sleeved with mounting sleeves used for wrapping the air inlet pipe and the sleeve, and external threads are arranged on the outer walls of the mounting sleeves; a stepped hole is formed in the sleeve, and the stepped hole is composed of a large-diameter through hole in the middle and small-diameter through holes respectively communicated with the upper end and the lower end of the large-diameter through hole; the opening and closing assembly comprises a pushing rod penetrating through the stepped hole, a plugging disc arranged at the lower end of the pushing rod and a pressing disc arranged at the upper part of the pushing rod; the upper end of the push rod extends to the outside of the sleeve; the pressing disc is positioned in the large-diameter through hole; the blocking disc is positioned in the air inlet pipe and used for blocking the lower end of the sleeve; the push rod is sleeved with a return spring on the part positioned in the large-diameter through hole, and the upper end of the return spring is fixedly connected with the lower surface of the pressing disc.

Further, an inflation mechanism is detachably connected outside the mounting sleeve; the inflation mechanism comprises an injector and a connecting sleeve arranged outside the nipple of the injector; the length of the connecting sleeve is greater than that of the nipple, and an internal thread matched with the external thread of the mounting sleeve is arranged on the inner wall of the connecting sleeve; a pressure lever is arranged in the connecting sleeve and below the nipple; when the connecting sleeve is in threaded connection with the mounting sleeve, the push rod is pressed downwards by the pressure rod, and the plugging disc is separated from the lower end of the sleeve.

Further, a rubber pad is arranged on the inner wall of the conical through hole.

Furthermore, heating coils are arranged on the side wall of the incubator body between the first supporting disc and the second supporting disc and/or on the upper surface of the isolation plate; the heating coil is arranged around the rotating rod.

Furthermore, the upper end of the push rod is provided with a pressing block.

Further, a liquid discharge pipe is arranged at the bottom of the heating area.

The utility model has the advantages that: the centrifuge tube of the utility model passes through the air bag, and then the lower end of the centrifuge tube extends into the conical through hole, the lower end of the centrifuge tube is clamped in the conical through hole, the upper part of the centrifuge tube can be clamped due to certain elasticity of the air bag, and the centrifuge tube is driven to rotate by the motor due to the arrangement of the motor, so that the process of shaking the centrifuge tube to accelerate cell fusion is realized, and a constant temperature environment is provided for the centrifuge tube by the heating medium due to the heating area in the centrifuge tube;

in addition, the edge of the first support disc is clamped in the annular groove, and the liquid level of the heating medium is lower than that of the first support disc, so that the heating medium cannot splash everywhere when the centrifugal tube rotates due to the blockage of the first support disc;

finally, owing to be provided with on the annular gasbag and fill gassing device, consequently the gasbag can be applicable to more extensive centrifuging tube size scope, when needs aerify annular gasbag, will aerify the mechanism and connect and put in the installation cover, then press the piston rod of syringe both can aerify the gasbag to its centrifuging tube that presss from both sides tight size is less, when needs are to the gasbag gassing, presses the catch bar, both can emit the inside part gas of gasbag, so that the centrifuging tube that the adaptation size is great or press from both sides tight in it takes out.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is a schematic structural view of the present invention when the inflation mechanism is connected;

fig. 3 is a partial enlarged view of the point a of the present invention;

fig. 4 is a top view of the present invention;

FIG. 5 is a schematic structural view of the inflation mechanism of the present invention;

reference numerals: 1-a thermostat body; 2-a separation plate; 3, a motor; 4-a first support disc; 5-a second support disc; 6-centrifuging the tube; 7-heating coils; 8-a tapered through hole; 9-rubber pad; 10-water through holes; 11-mounting holes; 12-an annular balloon; 13-an air inlet pipe; 14-a sleeve; 15-large diameter through holes; 16-small diameter through holes; 17-a pressing plate; 18-plugging disc; 19-a return spring; 20-a push rod; 21-a pressure bar; 22-pressing block; 23-nipple; 24-a syringe; 25-connecting sleeves; 26-a liquid inlet; 27-rotating rods; 28-liquid discharge pipe; 29-an annular groove; and 30-mounting a sleeve.

Detailed Description

The technical solutions of the present invention will be further described below with reference to the accompanying drawings and examples, so that those skilled in the art can practice the solutions.

Example 1:

as shown in fig. 1 and fig. 4, the thermostat for cell fusion of the present invention comprises a thermostat body 1, wherein a partition plate 2 is disposed at a lower portion of the thermostat body 1, and the partition plate 2 divides the thermostat body 1 into an upper heating area and a lower driving area; a motor 3 is arranged in the driving area, an output shaft of the motor 3 is connected with a rotating rod 27, and the rotating rod 27 is rotatably connected with the isolation plate 2 and extends into the heating area; the part of the rotating rod 27 positioned in the heating area is sequentially provided with a first supporting disk 4 and a second supporting disk 5 from top to bottom; heating coils 7 are arranged on the side wall of the incubator body 1 between the first supporting disk 4 and the second supporting disk 5 and on the upper surface of the isolation plate 2; the heating coil 7 is arranged around the swivelling levers 27. A liquid inlet 26 is arranged on the first supporting disc 4; the bottom of the heating zone is provided with a drain 28. An annular groove 29 is formed in the inner wall of the incubator body 1, and the edge of the first support disc 4 is clamped in the annular groove 29; a protective door is hinged on the liquid inlet 26. A heating medium is placed in the heating zone; the level of the heating medium is located below the first support tray. The diameter of the second supporting disk 5 is smaller than the inner diameter of the incubator body 1, and a plurality of water through holes 10 for water permeation are further formed in the second supporting disk.

A plurality of balls can be embedded in the annular groove and are abutted to the lower surface of the first supporting disc, so that the balls can play a supporting role and can reduce friction force when the first supporting disc rotates. In addition, the edge of the first supporting disc is embedded in the annular groove, so that a certain splash-proof effect can be achieved on liquid below the first supporting disc, and heating media below the first supporting disc are prevented from splashing out of the thermostat body due to rotation when the first supporting disc rotates. In addition, the liquid inlet is provided with a protective door, so that the protective effect can be achieved while the heating medium can be added into the thermostat body, and the heating medium is prevented from splashing out of the liquid inlet. The protective door can be also provided with a plurality of exhaust holes to prevent the air pressure in the heating area from being too high.

The utility model discloses a heating coil sets up in two regions, consequently can guarantee that the temperature about the second supporting disk is unanimous, can not influence the homogeneity of its inside temperature because of being provided with the second supporting disk.

The temperature control in the thermostat, i.e. the feedback of signals between the temperature sensor and the heating coil, is a common practice in water baths in the prior art, and is not an improvement of the present invention, so that the description thereof is not repeated here.

The first supporting disk 4 is symmetrically provided with at least two mounting holes 11 for placing centrifuge tubes 6, the second supporting disk 5 is provided with tapered through holes 8 for supporting the lower parts of the centrifuge tubes 6, and the tapered through holes 8 are arranged in one-to-one correspondence with the mounting holes 11; and an annular air bag 12 for tightly propping the centrifugal tube 6 is arranged on the inner wall of the mounting hole 11. And a rubber pad 9 is arranged on the inner wall of the conical through hole 8.

The utility model discloses be provided with toper through-hole and mounting hole, consequently when the centrifuging tube was placed in the mounting hole, its lower extreme can pass the toper through-hole, because the diameter of toper through-hole down reduces gradually from last, consequently can conveniently block the lower part of centrifuging tube, in addition, be provided with annular gasbag in the mounting hole, because annular gasbag has certain elasticity, consequently can be with the centrifuging tube chucking in certain diameter range among the annular gasbag. In addition, the conical through hole and the mounting hole of the utility model can also adopt an inclined arrangement mode, for example, after the centrifugal tube is mounted, the whole body is inclined to a certain intersection bottom; finally, in order to facilitate observation, the first supporting disk and the second supporting disk can be made of transparent materials.

During the use, with the centrifuge tube chucking in mounting hole and toper through-hole, then starter motor makes the centrifuge tube rotatory, because the centrifuge tube part immerses in the constant temperature environment down, both can be to the centrifuge tube heating and can make its interior liquid rock in order to accelerate its process of fusing again.

Example 2:

as shown in fig. 1 to 5, the present embodiment is different from embodiment 1 in that an inflation/deflation device is provided on the upper surface of the annular airbag 12; the inflation and deflation device comprises an air inlet pipe 13 communicated with the inside of the annular air bag 12, a sleeve 14 connected to the upper end of the air inlet pipe 13 and an opening and closing assembly positioned in the sleeve 14 and used for opening and closing the air inlet pipe 13; the lower end of the sleeve 14 extends into the air inlet pipe 13, and the outer wall of the sleeve 14 is connected with the air inlet pipe 13 in a sealing manner; the air inlet pipe 13 and the sleeve 14 are externally sleeved with an installation sleeve 30 used for wrapping the air inlet pipe 13 and the sleeve 14, and the outer wall of the installation sleeve 30 is provided with external threads; a stepped hole is formed in the sleeve 14, and the stepped hole is composed of a large-diameter through hole 15 in the middle and small-diameter through holes 16 respectively communicated with the upper end and the lower end of the large-diameter through hole 15; the opening and closing assembly comprises a push rod 20 penetrating through the stepped hole, a plugging disc 18 arranged at the lower end of the push rod 20 and a pressing disc 17 arranged at the upper part of the push rod 20; the upper end of the push rod 20 is provided with a pressing block 22, and the upper end of the push rod extends to the outside of the sleeve 14; the pressing disc 17 is positioned in the large-diameter through hole 15; the plugging disc 18 is positioned in the air inlet pipe 13 and is used for plugging the lower end of the sleeve 14; the part of the push rod 20, which is positioned in the large-diameter through hole 15, is sleeved with a return spring 19, and the upper end of the return spring 19 is fixedly connected with the lower surface of the pressing disc 17.

The utility model discloses an open and close the subassembly, when pressing the catch bar, the catch bar drives and presses pressure dish and shutoff dish to move down to make the shutoff dish leave telescopic lower extreme entry, and reset spring is in compression state this moment, gas in the annular gasbag can be followed the sleeve and spilled over, thereby makes the annular gasbag reduce. When loosening the catch bar, reset spring extension for press the dish rebound, thereby drive catch bar and shutoff dish and shift up simultaneously, die with telescopic lower extreme minor diameter through-hole shutoff, consequently gaseous unable spilling over in the annular gasbag. The utility model discloses a set up the aeration quantity that the subassembly was opened and close and can be changed in the annular gasbag to make its centrifuging tube that adapts to more different diameters.

An inflation mechanism is detachably connected outside the mounting sleeve 30; the inflation mechanism comprises an injector 24 and a connecting sleeve 25 arranged outside the nipple 23 of the injector 24; the length of the connecting sleeve 25 is greater than that of the nipple 23, and the inner wall of the connecting sleeve is provided with an internal thread matched with the external thread of the mounting sleeve 30; a pressure lever 21 is arranged in the connecting sleeve 25 and below the nipple 23; when the connecting sleeve 25 is screwed on the mounting sleeve 30, the push rod 20 is pressed downwards by the press rod 21, and the plugging disc 18 is separated from the lower end of the sleeve 14.

The utility model discloses an aerify the mechanism and can realize the operation of aerifing to annular gasbag, when needs aerify annular gasbag, will aerify the adapter sleeve threaded connection of mechanism and sheathe in the installation, when down rotatory adapter sleeve, the depression bar can compress tightly and make it move down in the catch bar top to will open and close the subassembly and open, thereby in the piston rod of pressing the syringe with the gaseous annular gasbag that impels in the syringe, will place the centrifuging tube top in annular gasbag tight. After the inflation, upwards rotate the adapter sleeve to make and open and close the subassembly and close, the gas in the annular gasbag can not be excessive, simultaneously with the upper end chucking of centrifuge tube. When the centrifuging tube was taken off to needs, can press the catch bar with the hand, opened and close the subassembly, annular gasbag loosens, and the centrifuging tube can be taken out.

Of course, the above description is not intended to limit the present invention, and the present invention is not limited to the above examples, and the changes, modifications, additions or replacements made by those skilled in the art within the scope of the present invention also belong to the protection scope of the present invention.

Claims

1. A thermostatic device for cell fusion, includes thermostated container body (1), its characterized in that: the lower part of the incubator body (1) is provided with an isolation plate (2), and the isolation plate (2) divides the incubator body (1) into a heating area positioned above and a driving area positioned below; a heating medium is placed in the heating zone; a motor (3) is arranged in the driving area, an output shaft of the motor (3) is connected with a rotating rod (27), and the rotating rod (27) is rotatably connected with the isolating plate (2) and extends into the heating area; the part of the rotating rod (27) positioned on the heating area is sequentially provided with a first supporting disc (4) and a second supporting disc (5) from top to bottom; the first supporting disk (4) is symmetrically provided with at least two mounting holes (11) for placing centrifuge tubes (6), the second supporting disk (5) is provided with conical through holes (8) for supporting the lower parts of the centrifuge tubes (6), and the conical through holes (8) are arranged in one-to-one correspondence with the mounting holes (11); a liquid inlet (26) is formed in the first supporting disc (4); and an annular air bag (12) for tightly propping the centrifugal tube (6) is arranged on the inner wall of the mounting hole (11).

2. A thermostatic device for cell fusion according to claim 1, wherein: an annular groove (29) is formed in the inner wall of the incubator body (1), and the edge of the first support disc (4) is clamped in the annular groove (29); a protective door is hinged on the liquid inlet (26); the diameter of the second supporting disk (5) is smaller than the inner diameter of the thermostat body (1), and a plurality of water through holes (10) for water permeation are further formed in the second supporting disk.

3. A thermostatic device for cell fusion according to claim 1, wherein: the upper surface of the annular air bag (12) is provided with an air charging and discharging device; the inflation and deflation device comprises an air inlet pipe (13) communicated with the inside of the annular air bag (12), a sleeve (14) connected to the upper end of the air inlet pipe (13), and an opening and closing assembly positioned in the sleeve (14) and used for opening and closing the air inlet pipe (13); the lower end of the sleeve (14) extends into the air inlet pipe (13), and the outer wall of the sleeve (14) is connected with the air inlet pipe (13) in a sealing manner; the air inlet pipe (13) and the sleeve (14) are externally sleeved with mounting sleeves (30) used for wrapping the air inlet pipe (13) and the sleeve (14), and external threads are arranged on the outer walls of the mounting sleeves (30); a stepped hole is formed in the sleeve (14), and the stepped hole is composed of a large-diameter through hole (15) in the middle and small-diameter through holes (16) respectively communicated with the upper end and the lower end of the large-diameter through hole (15); the opening and closing assembly comprises a push rod (20) penetrating through the stepped hole, a blocking disc (18) arranged at the lower end of the push rod (20) and a pressing disc (17) arranged at the upper part of the push rod (20); the upper end of the push rod (20) extends to the outside of the sleeve (14); the pressing disc (17) is positioned in the large-diameter through hole (15); the blocking disc (18) is positioned in the air inlet pipe (13) and is used for blocking the lower end of the sleeve (14); the part of the push rod (20) positioned in the large-diameter through hole (15) is sleeved with a return spring (19), and the upper end of the return spring (19) is fixedly connected with the lower surface of the pressing disc (17).

4. A thermostatic device for cell fusion according to claim 3, wherein: the outside of the mounting sleeve (30) is detachably connected with an inflating mechanism; the inflation mechanism comprises an injector (24) and a connecting sleeve (25) arranged outside the nipple (23) of the injector (24); the length of the connecting sleeve (25) is greater than that of the nipple (23), and an inner thread matched with the outer thread of the mounting sleeve (30) is arranged on the inner wall of the connecting sleeve; a pressure lever (21) is arranged in the connecting sleeve (25) and below the nipple (23); when the connecting sleeve (25) is in threaded connection with the mounting sleeve (30), the push rod (20) is pressed downwards by the pressure rod (21), and the plugging disc (18) leaves the lower end of the sleeve (14).

5. A thermostatic device for cell fusion according to claim 1, wherein: and a rubber pad (9) is arranged on the inner wall of the conical through hole (8).

6. A thermostatic device for cell fusion according to claim 1, wherein: a heating coil (7) is arranged on the side wall of the incubator body (1) between the first supporting disk (4) and the second supporting disk (5) and/or on the upper surface of the isolation plate (2); the heating coil (7) surrounds the rotating rod (27).

7. A thermostatic device for cell fusion according to claim 3, wherein: the upper end of the push rod (20) is provided with a pressing block (22).

8. A thermostatic device for cell fusion according to claim 1, wherein: and a liquid discharge pipe (28) is arranged at the bottom of the heating area.