CN111921230A - A kind of chromatographic column auxiliary liquid addition device and liquid addition method - Google Patents

Abstract

The application relates to a device for adding an eluent for a chromatography column, which belongs to the field of laboratory experimental instruments. The device includes four parts: a vent pipe, a conical flow blocking cap, a card bamboo shoot, and a valve. The device can be used alone, or it can be used with commercially available ordinary funnels. When adding liquid, put the chromatographic column auxiliary liquid addition device on the chromatographic column or the chromatographic column equipped with a common funnel, open the valve, and pour the eluent into the funnel. When there is no need to add a large amount of liquid, close the valve. The application and the liquid addition method can slowly, continuously and stably add the eluent, protect the filler interface, and reduce the operating burden of the experimenter. After the sample is loaded, there is no need to repeatedly draw a circle on the inner wall of the column to add the eluent. The structure is simple, and it is suitable for the market. There are chromatography columns and funnels, which can use less or no quartz sand, and the use cost is low.

Description

A kind of chromatographic column auxiliary liquid addition device and liquid addition method

technical field

The application relates to the field of laboratory experimental instruments, in particular to a chromatographic column auxiliary liquid addition device and a liquid addition method.

Background technique

Column chromatography is a commonly used method for separating sample components in chemical laboratories. Chromatography column is the main experimental instrument in column chromatography. Adding eluent is an important experimental step in column chromatography. The addition requires slow, continuous and steady addition without interruption or damage to the smooth surface of the adsorbent.

At present, the dropper or funnel is often added along the inner wall of the column, and the mouth of the dropper is attached to the inner wall of the column, so that the eluent is injected along the inner wall of the column. Inefficient. Even for experienced experimenters, it is difficult to ensure that the filler is always flat and uniform without being washed out using a dropper. When using the funnel to feed the liquid, it is required that the column be filled with quartz sand or a sufficient amount of eluent. During operation, it can only be added along one side of the inner wall of the column, and the liquid cannot be uniformly fed along the circumference of the inner wall, which is not conducive to the separation of sample components. . Therefore, neither the dropper nor the funnel can achieve the requirements of slow, continuous, stable, non-interrupted and non-destructive filling surface level.

CN108714320A discloses a chromatography device capable of automatic liquid feeding, comprising a chromatography column, a spherical injection port container, a speed-regulated peristaltic pump and a pressurized pump. Fixed cylindrical tube. The eluent can be added to the column through the flow hole in the cylindrical tube wall. The function of the invention optimizes the combination of multiple components, which can protect the surface of the filler to a certain extent, but the structure is cumbersome and needs to be used with a special chromatography column, which is not conducive to wide-scale promotion.

CN202876438U discloses a multi-stage column chromatography automatic liquid addition device, comprising a storage, a pressurizing system, a communication pipe, a chromatography column, a product collector and a support table. The first chromatographic column and the second chromatographic column are both provided with sand screens to prevent the eluent from damaging the surface of the filler. The utility model solves the problem of continuous liquid addition. However, the problem of picking and placing sand nets is difficult to solve.

CN207153163U discloses a chromatography column auxiliary liquid addition device, which comprises a chromatography column and a chromatography liquid addition funnel movably connected above the chromatography column, the chromatography liquid addition funnel comprises a funnel main body and a closed lower end and is connected with the funnel main body A fixed cylindrical funnel tube, the upper end of the cylindrical funnel tube is communicated with the funnel main body, the outer conical surface of the funnel main body is in contact with the upper end of the chromatography column, the cylindrical funnel tube is inserted into the chromatography column, and the cylindrical funnel tube tube The wall is provided with a plurality of flow holes for the added liquid to reach the inner wall of the chromatographic column after being ejected. The utility model has simple structure and good universality. However, when the eluent is too much or too little, it will cause problems such as poor liquid flow and direct flushing of the filler.

SUMMARY OF THE INVENTION

In view of the problem in the prior art that the use of droppers or funnels cannot achieve slow, continuous, stable, uninterrupted and non-destructive liquid filling requirements, the application provides a chromatography column auxiliary liquid addition device and liquid addition device The method has a simple structure, is suitable for existing chromatographic columns and funnels in the market, can protect the smoothness of the packing layer, and achieve the purpose of rapidly adding the eluent.

A chromatographic column auxiliary liquid addition device of the present application includes four parts: a vent pipe, a conical flow blocking cap, a card bamboo, and a valve. The part of the conical flow blocking cap is in the shape of a hat with a hole at the tip of the cap, and the bottom edge has a number of tentacles, and the conical flow blocking cap is connected with the lower part of the ventilation pipe. The snap-on portion is located above the conical flow blocking cap portion and is connected with the middle portion of the ventilation pipe. The valve portion is located in the upper part of the vent pipe. The chromatographic column auxiliary liquid addition device can be used with a chromatographic column equipped with a common funnel, or the funnel can be used as an internal component of the chromatographic column auxiliary liquid addition device and used with the chromatography column.

A liquid addition method utilizing a chromatographic column auxiliary liquid addition device, comprising:

Step 1: Put the chromatographic column auxiliary liquid addition device on the chromatographic column or on the chromatographic column equipped with a common funnel;

Step 2: Open the valve;

Step 3: Pour the rinse agent into the funnel;

Step 4: When there is no need to add a lot of liquid, close the valve.

Among them, in the process of use, only steps 1 and 3 are included, and the addition of the eluent can also be completed independently.

Compared with the prior art, the advantages of the present application are:

(1) The application and the liquid addition method can slowly, continuously and stably add the eluent to protect the filler interface;

(2) Through the valve, it can be added and used at any time, and it can be stopped when it is closed, and it acts like a constant pressure funnel to avoid the generation of air bubbles above the packing due to low pressure;

(3) The application is applied at the grinding port of the chromatography column, not extending into the chromatography column, avoiding falling on the filler, and being easy to pick and place;

(4) To reduce the operational burden of the experimental staff, after the sample is loaded, there is no need to repeatedly drop the eluent on the fresco circle in the column;

The structure is simple, suitable for existing chromatography columns and funnels in the market, and can use less or no quartz sand, and the use cost is low.

Description of drawings

Fig. 1 is the structural representation of common funnel;

2 is a schematic diagram of the overall structure of a chromatography column auxiliary liquid addition device provided in an embodiment of the present application;

3 is a schematic cross-sectional view of a chromatography column auxiliary liquid addition device provided in an embodiment of the present application;

Fig. 4 is a kind of chromatographic column auxiliary liquid adding device card bamboo structure schematic diagram provided by the embodiment of this application;

FIG. 5 is a schematic cross-sectional view of a chromatography column auxiliary liquid addition device according to an embodiment of the present application.

Description of the symbols in the figure: 00A- ordinary funnel; 00B- small-capacity funnel; 00C- large-capacity funnel; 10A-straight ventilation pipe; 10B-curved ventilation pipe; 200-conical flow blocking cap; Horizontal card shoots; 30B-claw card shoots; 400-valve.

Detailed ways

The present invention will be further described in detail with reference to FIGS. 1 to 5 and through the embodiments. The following embodiments are intended to explain the present invention and the present invention is not limited to the following embodiments.

Example 1

As shown in Figure 1, the common funnel 00A is usually made of glass, with a frosted outside of the small opening at the bottom and a smooth inside.

As shown in FIG. 2 , an auxiliary liquid addition device for a chromatographic column includes four parts: a straight vent pipe 10A, a conical flow blocking cap 200 , a horizontal card bamboo 30A, and a valve 400 . The chromatographic column auxiliary liquid addition device is used with the common funnel 00A. The conical baffle cap 200 is partially in the shape of a hat with a hole at the tip of the cap. The bottom edge of the conical flow blocking cap 200 has a number of antennae 201, the antennae 201 protrude 0.1-3mm from the bottom edge of the conical flow blocking cap 200, and form a downward flow of the eluent between the conical flow blocking cap 200 and the common funnel 00A gap. The conical baffle cap 200 is connected to the lower part of the straight ventilation pipe 10A. The horizontal snap 30A is partially located above the conical baffle cap portion 200 and is connected to the middle of the straight vent pipe 10A. The horizontal snap 30A is similar to a propeller and can be elastically deformed in the horizontal direction to achieve a fixing aid. For the effect of the liquid adding device, the horizontal card bamboo 30A has three or more blades. The valve portion 400 is located at the upper portion of the straight ventilation pipe 10A. The chromatographic column auxiliary liquid addition device is made of polytetrafluoroethylene and other anti-corrosion materials with certain elastic deformation ability.

A liquid addition method utilizing a chromatographic column auxiliary liquid addition device, comprising:

Step 1: Put the chromatographic column auxiliary liquid addition device on the chromatographic column equipped with a common funnel;

Step 2: Open the valve;

Step 3: Pour the rinse agent into the funnel;

Step 4: When there is no need to add a lot of liquid, close the valve.

Among them, in the process of use, only steps 1 and 3 are included, and the addition of the eluent can also be completed independently.

Example 2

As shown in Figure 3, a chromatography column auxiliary liquid addition device includes five parts: a small-capacity funnel 00B, a straight vent pipe 10A, a conical flow blocking cap 200, a claw-shaped snap-on 30B, and a valve 400. The small-capacity funnel 00B and the Other parts of the chromatographic column auxiliary liquid addition device cannot be disassembled. The conical baffle cap 200 is partially in the shape of a hat with a hole at the tip of the cap. The bottom edge of the conical flow blocking cap 200 has a number of antennae 201 (not marked in the figure), the antennae 201 protrude 0.1-3mm from the bottom edge of the conical flow blocking cap 200, and the conical flow blocking cap 200 and the small capacity Between the funnels 00B, the downward gap of the eluent is formed. The conical baffle cap 200 is connected to the lower part of the straight ventilation pipe 10A. The claw-shaped snap-on 30B is partially located above the conical flow blocking cap portion 200 and is connected with the middle of the straight vent pipe 10A. The claw-shaped snap-on 30B can be elastically deformed in the vertical direction to achieve a fixed auxiliary increase. To achieve the effect of the liquid device, the claw-type snap-on 30B has three or more claws. The valve portion 400 is located at the upper portion of the straight ventilation pipe 10A. The chromatographic column auxiliary liquid addition device is made of polytetrafluoroethylene and other anti-corrosion materials with certain elastic deformation ability.

As shown in FIG. 4 , which is a schematic diagram of the independent structure of the claw-type snap-on 30B, the claw-type snap-on 30B is directly connected with the straight ventilation pipe 10A and cannot be disassembled.

A liquid addition method utilizing a chromatographic column auxiliary liquid addition device, comprising:

Step 1: Put the auxiliary liquid dosing device of the chromatography column on the grinding port of the chromatography column;

Step 2: Open the valve;

Step 3: Pour the rinse agent into the funnel;

Step 4: When there is no need to add a lot of liquid, close the valve.

Among them, in the process of use, only steps 1 and 3 are included, and the addition of the eluent can also be completed independently.

Example 3

As shown in Figure 5, a chromatography column auxiliary liquid addition device includes a small-capacity funnel 00C, a curved vent tube 10B, a conical flow blocking cap 200, and a valve 400. The large-capacity funnel O0C and the chromatography column auxiliary Other parts of the dosing device are not removable. The conical baffle cap 200 is partially in the shape of a hat with a hole at the tip of the cap. The bottom edge of the conical flow blocking cap 200 has a number of antennae 201 (not marked in the figure), the antennae 201 protrude from the bottom edge of the conical flow blocking cap 200 by 0.1-3mm, and the conical flow blocking cap 200 and the large capacity The 00C between the funnels forms the downward gap of the eluent. The conical baffle cap 200 is connected to the lower part of the curved ventilation pipe 10B. The valve portion 400 is located at the upper portion of the curved vent pipe 10B. The chromatographic column auxiliary liquid addition device is made of polytetrafluoroethylene and other anti-corrosion materials with certain elastic deformation ability.

A liquid addition method utilizing a chromatographic column auxiliary liquid addition device, comprising:

Step 1: Put the auxiliary liquid dosing device of the chromatography column on the grinding port of the chromatography column;

Step 2: Open the valve;

Step 3: Pour the rinse agent into the funnel;

Step 4: When there is no need to add a lot of liquid, close the valve.

Among them, in the process of use, only steps 1 and 3 are included, and the addition of the eluent can also be completed independently.

The present application and the embodiments thereof have been described above schematically, and the description is not restrictive, and what is shown in the accompanying drawings is only one of the embodiments of the present application, and the actual structure is not limited thereto. Therefore, if those of ordinary skill in the art are inspired by it, and without departing from the purpose of the present invention, any structural modes and embodiments similar to this technical solution are designed without creativity, and all should belong to the protection scope of the present application.

Claims (10)

1. a chromatographic column auxiliary liquid addition device, is characterized in that, comprises: Breathing pipe, conical blocking cap, card bamboo, valve part; The part of the conical flow blocking cap is in the shape of a bucket hat with a hole in the tip of the cap; the bottom edge of the conical flow blocking cap part has a number of tentacles; The tentacles protrude from the bottom edge of the conical flow blocking cap by 0.1-3 mm, forming a downward gap for the eluent between the conical flow blocking cap and the funnel; the conical flow blocking cap is connected with the lower part of the ventilation pipe; The snap-on part is located above the conical flow blocking cap part, and is connected with the middle part of the ventilation pipe; The valve portion is located in the upper part of the vent pipe. 2. A chromatography column auxiliary liquid addition device according to claim 1, characterized in that, it can be used in conjunction with a chromatography column equipped with a common funnel. 3 . The auxiliary liquid adding device for a chromatography column according to claim 1 , wherein the card bamboo shoots can be horizontal card bamboo shoots or claw shaped card shoots. 4 . 4 . The auxiliary liquid addition device for a chromatography column according to claim 1 , wherein the material can be an anti-corrosion material with a certain elastic deformation ability such as polytetrafluoroethylene. 5 . 5. a kind of chromatographic column auxiliary liquid adding device according to claim 1, is characterized in that, the ventilation pipe can be straight type ventilation pipe to be fixed to the funnel wall vertically upward through the card bamboo, and the curved type ventilation pipe is fixed to the funnel wall . 6. a liquid adding method of chromatography column auxiliary liquid adding device as claimed in claim 1,2, is characterized in that, described liquid adding method is as follows: Step 1: Put the chromatographic column auxiliary liquid addition device on the chromatographic column equipped with a common funnel; Step 2: Open the valve; Step 3: Pour the rinse agent into the funnel; Step 4: When there is no need to add a lot of liquid, close the valve. 7. A chromatographic column auxiliary liquid adding device is characterized in that, comprising: Funnel, vent pipe, conical blocking cap, card bamboo, valve part; The part of the conical flow blocking cap is in the shape of a bucket hat with a hole in the tip of the cap; the bottom edge of the conical flow blocking cap part has a number of tentacles; The tentacles protrude from the bottom edge of the conical flow blocking cap by 0.1-3 mm, forming a downward gap for the eluent between the conical flow blocking cap and the funnel; the conical flow blocking cap is connected with the lower part of the ventilation pipe; The snap-on part is located above the conical flow blocking cap part, and is connected with the middle part of the ventilation pipe; The valve portion is located in the upper part of the vent pipe. 8. A chromatography column auxiliary liquid addition device according to claim 7, wherein the funnel is a large-capacity funnel. 9 . The chromatographic column auxiliary liquid addition device according to claim 7 , wherein the funnel is a small-capacity funnel. 10 . 10. a liquid addition method of the chromatographic column auxiliary liquid addition device as claimed in claim 7,8 or 7,9, is characterized in that, described liquid addition method is as follows: Step 1: Put the chromatographic column auxiliary liquid addition device on the chromatographic column; Step 2: Open the valve; Step 3: Pour the rinse agent into the funnel; Step 4: When there is no need to add a lot of liquid, close the valve.

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