CN216274173U - A low-shear air-lift fermenter oxygenation and mixing device - Google Patents

Abstract

The utility model provides an oxygen-increasing and mixing device for a low-shear force air-lift fermenter. In this technical solution, in addition to the intake air distributor, the airlift fermentation tank is also equipped with multiple rounds of air stones, which can adjust the direction of the multi-round air stones and the spout of the air guide cup to adjust the circulation of the gas-liquid flow along the guide cylinder. direction. After the microbubble flow is sprayed into the fermentation tank, the formed gas-liquid flow circulates under the guidance of the guide tube to realize the mixing of the fermentation liquid and the bacteria. The air distributor is installed at the discharge port at the bottom of the tank, including the bottom air stone and metal cover. The nano-to-micron diameter bubbles ejected by the air stone can effectively increase the dissolved oxygen concentration of the fermentation tank, and the dissolved oxygen level of the fermentation liquid and the material mixing can be controlled by adjusting the air intake of the air stone and the air distributor. On the premise of ensuring the dissolved oxygen level in the fermentation process, on the one hand, the energy consumption is greatly reduced, and on the other hand, the shear force damage to the cultured microorganisms or animal and plant cells caused by the traditional airlift fermenter to increase the dissolved oxygen vigorous aeration is reduced.

Description

A low-shear air-lift fermenter oxygenation and mixing device

technical field

The utility model belongs to the field of biological engineering, and relates to an air-lift fermenter fermentation liquid oxygenation device, in particular to a device for increasing oxygen and promoting material mixing in the culture of shear-sensitive microalgae cells, animal cells and plant cells .

Background technique

The airlift fermentation tank has many advantages such as simple structure, low energy consumption, high oxygen supply efficiency and high fluidization efficiency. The air-lift fermenters that have been widely used in the biological industry include air-lift inner circulation fermenters, gas-liquid double jet air-lift circulation fermenters, and tower-type air-lift fermenters with multi-layer distribution plates. The high-speed air is sprayed from the air nozzle, and the air is dispersed in the liquid in the form of bubbles. On the ventilated side, the average density of the liquid decreases. On the non-ventilated side, the liquid density is higher, so the density of the liquid on the ventilated side is generated. difference, thereby forming a circulation of the liquid in the fermenter. However, for some cultures such as microalgae cells, animal cells and plant protoplasts, they are more sensitive to shear force due to the large cells and no cell wall protection. The increased high-speed air flow will cause damage to the above culture, which limits the applicability of the traditional airlift fermenter in the above cell culture.

Through patent search, it is found that the improvement of air-lift fermenters focuses on adding or improving stirring devices and defoaming devices to improve the efficiency of dissolved oxygen and process control (such as CN208151359U, CN208151345U, CN208151345U, CN209923330U, CN105219618B, CN112358952A, CN202576406U, CN211947U, CN207646189U)，或改进挡板或导流管结构增加环流与混合效率(如CN211522177U，CN210620795U， CN102796650A，CN111662803A，CN105039150B，CN209010511U，CN208667695U， CN210886007U)，改进空气分布器结构(如CN204569910U，CN203754704U，CN102250752B， CN105602837A , CN210048760U, CN211847927U), enhance the fermentation parameter process control ability, such as adding PLC controller or light, sound, magnetic control system (such as CN108485951A,) or comprehensively transform the structure of the fermentation tank to increase the mixing efficiency (CN209412225U). In order to increase the mixing efficiency of dissolved oxygen and fermentation broth, the above air-lift fermenters all lead to the increase of liquid shear force, so they are not suitable for the cell fermentation process that is sensitive to shear force.

Compared with the above-mentioned patents, the present application uses an air stone that can eject bubbles of nanometer to micrometer diameter for gas supply. Under the condition of low-intensity aeration, the gas-liquid contact surface is greatly increased. Compared with the traditional airlift fermentation tank , After the utility model is installed, under the condition of the same intensity of ventilation, the gas-liquid contact surface is greatly increased, the dissolved oxygen efficiency is significantly improved, and the introduction of high-intensity airflow is avoided, thereby avoiding the occurrence of excessive shear force; On the other hand, by introducing the bottom airstone, the mixing efficiency of the fermentation broth can be greatly enhanced. The number of air stones and the number of connecting tube rounds of the fixed air stones can be adjusted according to the aerobic degree of the fermenting cells. A top cover is added above the gas outlet at the bottom to guide the air flow direction, so that the settled cells can be resuspended into the gas-liquid circulation flow. The air-lift fermenter oxygenation and mixing device can significantly increase the dissolved oxygen and mixing efficiency, and greatly reduce the energy consumption of the air compressor.

Utility model content

The purpose of the utility model is to provide a traditional air-lift fermenter with a high dissolved oxygen and mixing efficiency, low shear force, low transformation cost, strong practicability and low energy consumption, and is suitable for the fermentation culture of microalgae, animal and plant cells. installation.

The solution to the technical problem of the present invention is as follows:

A low-shear force air-lift type fermenter oxygenation and mixing device, characterized in that it comprises a ring-shaped air stone (1), a connecting pipe (2), a gas guide cup (19), and a one-way air valve (10) , a gas distributor (6), a bottom air stone (23), a thread (20) and a heat-resistant sealing ring (21). The ring-column air stone (1) and the bottom air stone (23) are oxygen-enhancing air stones or air-pan stones or fish tank air atomizers or oxygen-increasing heads or bubble discs or air discs or oxygen pump sand heads or nano-aero-pan stones or nano-bubbles Stone or high dissolved oxygen cake or oxygen stone or other porous stone formed by sintering. The ring-column air stone (1) is vertically connected to the connecting pipe (2) through the thread (20), and the air stone can be oriented upward or downward or parallel or perpendicular to the tank bottom (3). The bottom air stone (23) is connected to the air outlet end of the connecting pipe (2) through threads (20), the air stone faces the metal cover sheet (24), and the connecting pipe is provided with a one-way air valve (6). A heat-resistant sealing ring (21) is arranged in the thread (20) for fixedly connecting the ring-column air stone (1) or the bottom air stone (23) and the connecting pipe (2). There is a metal gas guide cup (19) between the ring-shaped air stone (1) and the connecting pipe. According to the circulating flow direction of the required gas-liquid mixture along the guide cylinder, by adjusting the position of the thread welded on the connecting pipe, Adjust the direction of the gas stone and the opening of the gas guide cup. There is a round hole in the center of the bottom of the gas guide cup (19), which is sleeved and fixed on the thread.

Preferably, there are two groups of connecting pipes for fixing the ring-column airstone (1).

Preferably, there are 8 airstones (1) connected to each group of connecting pipes.

There is a one-way air valve on the connecting pipe. The gas distributor (6) comprises a bottom gas stone (23), a connecting pipe (2), a thread (20), a heat-resistant sealing ring (21), a metal cover sheet (24), a one-way gas valve (10) and a metal rod (twenty two).

Preferably, a circular metal cover sheet (24) larger than the diameter of the air outlet is fixed 0.5 cm above the air outlet at the bottom of the tank, and the circular metal cover sheet (24) is welded and fixed to the bottom of the pipe or the air outlet by three metal rods (22). superior. Play the role of guiding air.

The beneficial effects of the present utility model are as follows:

1. The device can be assembled on traditional fermentation tanks, with low cost of transformation and strong practicability. It only needs to add connecting pipes, threads, air stones, air cushions, guide plates, and air distribution at the bottom to the existing air supply system. Weld the metal sheet on the top of the device.

2. The bubbles ejected by this device are nano- to micron-sized bubbles, and the gas-liquid contact area is greatly increased, and the dissolved oxygen of the fermentation liquid can be effectively increased under lower air pressure, thereby greatly reducing the energy consumption of the air compressor. Due to the weak shear force of small bubble bursting, the damage to shear stress-sensitive cultures such as microalgae cells, animal cells, and plant cells is greatly reduced.

3. The device modifies the air distributor at the bottom of the traditional air-lift fermenter, which avoids the problem of weak gas mixing and cell sedimentation under lower air pressure, and achieves the purpose of improving dissolved oxygen and ensuring material mixing.

Description of drawings

1 is a schematic front view of the structure of the present utility model installed on an air-lift fermenter;

Fig. 2 is the structural representation of the ring column air stone in the utility model;

Fig. 3 is the structural representation of thread and heat-resistant sealing ring in the utility model;

4 is a schematic structural diagram of a gas distributor in the utility model;

5 is a side view of a schematic structural diagram of the present utility model installed on an airlift fermenter;

Fig. 6 is the structural representation of the end cover installed in the air-lift fermenter;

In the figure, 1. Ring-column gas stone, 2. Connecting pipe, 3. Tank bottom, 4. Guide tube, 5. Middle cooling jacket, 6. Gas distributor, 7. Outlet, 8. Outlet valve , 9. Bottom cooling jacket, 10. One-way air valve, 11. Feeding port, 12. Inoculation port, 13. Thermometer sleeve, 14. Sensor sleeve, 15. Sensor sleeve, 16. Sight hole, 17 .Sampling tube, 18. Flange, 19. Gas guide cup, 20. Thread, 21. Heat-resistant sealing ring, 22. Metal rod, 23. Bottom air stone, 24. Metal cover.

Detailed ways

The utility model will be further described below in conjunction with the embodiments, but the scope of protection of the utility model is not limited to the scope expressed by the embodiments. Anyone who is familiar with the related art can make slight changes and modifications without departing from the spirit and scope of the present invention. Therefore, the scope of the patent protection of the present invention is determined by the scope of the patent application attached to this specification.

The low-shear-force air-lift fermentor oxygenation and mixing device provided by the utility model is a device used to improve the oxygen-dissolving and mixing efficiency of the air-lift fermenter during low-shear cell culture.

As shown in Figures 1-6, the low-shear air-lift fermenter oxygenation and mixing device is installed on the air-lift fermenter, and includes a ring-shaped air stone (1), a connecting pipe (2), a gas guide cup ( 19), one-way air valve (10), air distributor (6), bottom air stone (23), thread (20) and heat-resistant sealing ring (21). The ring column air stone (1) and the bottom air stone (23) are oxygen-enhancing air stones. The ring-column air stone (1) is vertically connected to the connecting pipe (2) through the thread (20), and the air stone faces downward and is perpendicular to the bottom of the tank. The bottom air stone (23) is connected to the air outlet end of the connecting pipe (2) through threads (20), the air stone faces the metal cover sheet (24), and the connecting pipe is provided with a one-way air valve (6). A heat-resistant sealing ring (21) is provided in the thread (20) for fixedly connecting the ring-column air stone (1) with the bottom air stone (23) and the connecting pipe (2). There is a round hole at the bottom of the metal gas guide cup (19), which is sleeved on the thread connecting the ring-shaped air stone (1) and the connecting pipe. After the ring-shaped air stone and the connecting pipe are tightly connected, the metal gas guide cup is clamped and fixed. . There are two groups of connecting pipes for fixing the ring-shaped air stones (1); and eight ring-shaped air stones (1) are connected to each group of connecting pipes. There is a one-way air valve on the connecting pipe. The gas distributor (6) includes a bottom gas stone (23), a connecting pipe (2), a thread (20), a heat-resistant sealing ring (21), a metal cover sheet (24), a one-way gas valve (10) and a metal rod (twenty two). A circular metal cover sheet (24) larger than the diameter of the air outlet is fixed at 0.5cm above the air outlet at the bottom of the tank, and the circular metal cover sheet (24) is welded and fixed on the bottom of the tube or the air outlet by three metal rods (22). Play the role of guiding air.

The above-mentioned air-lift fermenter, in addition to the low-shear force air-lift fermenter oxygenation and mixing device, also comprises a tank body, a guide tube (4), a middle cooling jacket (5), a discharge port (7 ), outlet valve (8), bottom cooling jacket (9), feeding port (11), inoculation port (12), thermometer sleeve (13), pH sensor sleeve (14), dissolved oxygen sensor sleeve Tube (15), sight hole (16), sampling tube (17), flange (18).

The device is suitable for microalgae and animal cells without cell walls in the fermentation industry, such as shear-sensitive cultures such as plant protoplasts, such as Euglena slenderum, Vero cells, and paclitaxel protoplasts.

The operating instructions of this device are as follows:

As shown in Figures 1, 2, and 4, sterile compressed air is introduced into the fermentation tank through the connecting pipe, diffused by the ring-shaped air stone and the bottom air stone, and the formed tiny air bubbles are mixed with the fermentation liquid to form a low-density gas-liquid mixture; After the air bubbles are sprayed to the bottom of the tank, the gas flows back into the channel between the diversion cylinder and the tank wall and moves upward, and the fermentation liquid in the center of the diversion cylinder sinks to form a circulation to realize the mixing of the fermentation liquid; The tiny bubbles ejected from the air stone mix with the fermentation liquid and then move upward, and then move along the direction of the bottom of the tank after being diverted by the metal cover sheet. Cells are resuspended and mixed.

According to the reading of the dissolved oxygen sensor (15), under the condition that the ventilation volume of the connecting pipe (2) of the bottom air stone (23) is fixed, adjust the number of ventilation groups of the connecting pipe (2) of the annular air stone (1) and the ventilation rate of each group. The ventilation volume of the connecting pipe is used to control the dissolved oxygen level of the fermentation broth. When low dissolved oxygen is required, stop the air supply to the connecting pipe (2) of the ring-column air stone (1), and adjust the ventilation rate of the connecting pipe (2) of the bottom air stone (23) to control the level of dissolved oxygen and the material mixing and cell suspension. .

The above is only the preferred embodiment of the present invention. It should be pointed out that for those skilled in the art, without departing from the principle of the present invention, several improvements and modifications can be made. These improvements and Retouching should also be regarded as the protection scope of the present invention.

Claims (11)

1. The utility model provides a low shearing force airlift fermentation cylinder oxygenation and mixing arrangement which characterized in that: the gas distributor comprises a ring array air stone (1), a connecting pipe (2) for supplying gas to the ring array air stone and fixing the ring array air stone, a gas guide cup (19) positioned around the ring array air stone, a one-way gas valve (10) on the connecting pipe (2), a thread (20) for fixing the ring array air stone, a heat-resistant sealing ring (21) in the thread, a bottom air stone (23) positioned on a bottom vent pipe, a round metal cover plate (24) above the bottom air stone, and a gas distributor (6) consisting of the bottom air stone (23) and the round metal cover plate (24).

2. The aeration and mixing device of low shear force airlift fermentation tank of claim 1, wherein said air stones (1) and bottom air stones are aeration bubble stones or air disk stones or fish tank air atomizer or aeration head or bubble disk or air disk or oxygen pump sand head or nano air disk stones or high dissolved oxygen cake or oxygen stone or other porous stones formed by sintering.

3. The aeration and mixing apparatus of low shear airlift fermenter according to claim 1, wherein the ring of air stones (1) is vertically connected to the connecting pipe (2) by a screw thread (20), and the air stones are oriented upward or downward or parallel or vertical to the bottom (3) of the fermenter.

4. The aeration and mixing apparatus of low shear airlift fermenter according to claim 1, wherein the screw thread (20) for fixedly connecting the annular air stone (1) or the bottom air stone (23) and the connecting pipe (2) is provided with a heat-resistant sealing ring (21).

5. The aeration and mixing device of low shear force airlift fermentation tank of claim 1, wherein a metal gas diversion cup (19) is provided between the annular air stone (1) and the connecting pipe (2), and the orientation of the air stone head and the opening direction of the gas diversion cup are adjusted by adjusting the position of the thread welded on the connecting pipe according to the circulation flow direction of the required gas-liquid mixture along the diversion cylinder.

6. The aeration and mixing device of low shear airlift fermenter according to claim 1, wherein the bottom of the gas diversion cup (19) has a circular hole at its center, and is fixed on the screw thread by sleeving.

7. The aeration and mixing apparatus of low shear airlift fermenter according to claim 1, wherein the number of the connecting pipes for fixing the aerators (1) is two, but not limited to two, groups, and the number of the aerators (1) connected to each group of the connecting pipes is 8, but not limited to 8.

8. The aeration and mixing apparatus of low shear airlift fermentor according to claim 1, characterized in that the connecting tube is provided with a one-way air valve.

9. The low shear airlift fermentor oxygenation and mixing device of claim 1, characterized in that: the gas distributor (6) comprises a bottom air stone (23), a connecting pipe (2), a thread (20), a heat-resistant sealing ring (21), a metal cover plate (24), a one-way gas valve (10) and a metal rod (22).

10. The oxygenation and mixing device of low shear airlift fermentation tank of claim 1, wherein a circular metal cover (24) with diameter larger than that of the air outlet is fixed 0.1-3 cm above the air outlet at the bottom of the tank, and the circular metal cover is welded by three metal rods (22) and fixed on the bottom of the pipe or the air outlet to guide air.

11. The aeration and mixing apparatus of low shear airlift fermenter according to claim 1, wherein the bottom air stone (23) is connected to the air outlet end of the connecting pipe (2) by a screw thread (20), and the air stone head faces the metal cover plate (24).

Images