CN101985595A - Membrane type photobioreactor for enhancing microalgae culturing - Google Patents
Membrane type photobioreactor for enhancing microalgae culturing Download PDFInfo
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- 238000012258 culturing Methods 0.000 title description 3
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- 229910002092 carbon dioxide Inorganic materials 0.000 description 7
- 239000001569 carbon dioxide Substances 0.000 description 7
- 238000009825 accumulation Methods 0.000 description 6
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 6
- 235000011089 carbon dioxide Nutrition 0.000 description 6
- 239000001301 oxygen Substances 0.000 description 6
- 229910052760 oxygen Inorganic materials 0.000 description 6
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Abstract
本发明公开了一种强化微藻培养的膜式光生物反应器，包括外光源、底板以及底板上竖直设置的内套筒和外套筒，外套筒位于内套筒的外侧并将内套筒包围，外套筒顶端设有水平设置的透明的顶盖板，顶部设有若干出气口，底部设有出料口；内套筒底部设有若干交换孔，底板上设有被内套筒包围的进气口，出气口位于内套筒顶端上方，底板垂直设置有位于内套筒内侧的膜组件，膜组件的底部边缘与底板密封固定并将进气口包围。本发明的强化微藻培养的膜式光生物反应器结构简单、操作简便、性能稳定、具有较大的比表面积和光传递效率，膜组件的采用，使气体分布均匀，可单独使用或者与跑道池连用用于微藻放大培养。
The invention discloses a membrane-type photobioreactor for strengthening microalgae cultivation, which comprises an external light source, a base plate, and an inner sleeve and an outer sleeve vertically arranged on the base plate. The outer sleeve is located outside the inner sleeve and Surrounded by a sleeve, the top of the outer sleeve is provided with a horizontally arranged transparent top cover plate, with several air outlets on the top and a discharge port at the bottom; several exchange holes are provided at the bottom of the inner sleeve, and the inner sleeve is provided on the bottom plate. The air inlet surrounded by the barrel, the air outlet is located above the top of the inner sleeve, the bottom plate is vertically provided with a membrane module located inside the inner sleeve, the bottom edge of the membrane module is sealed and fixed with the bottom plate and surrounds the air inlet. The membrane-type photobioreactor for intensifying microalgae cultivation of the present invention has simple structure, easy operation, stable performance, large specific surface area and light transmission efficiency, and the adoption of membrane components makes the gas distribution uniform, and can be used alone or combined with a raceway pool Used in conjunction with microalgae amplification culture.
Description
Technical field
The present invention relates to algae culture reactor technology field, especially relate to a kind of membrane type bioreactor that little algae is cultivated of strengthening.
Background technology
Little algae be a class on land, the ocean is widely distributed, the high holophyte of nutritious, photosynthetic availability, the polysaccharide that cellular metabolism produces, protein, pigment etc. make it have good DEVELOPMENT PROSPECT in fields such as food, medicine, genetically engineered, liquid fuels.Advantages such as little algae is expected to crack a key of energy dilemma petroleum times as the back, and it is big to have production capacity, pollution-free, renewable, development prospect is very fine.But, and, also be absolutely necessary for the research and development of optical-biological reaction apparatus required in little algae culturing process because the little algae of domestic energy research and use and still be in the starting stage still has many problems to be difficult to solve.
The DESIGN OF REACTOR purpose of algae culture is to pursue bigger light-receiving area, eliminates the oxygen accumulation.The reactor of algae culture mainly contains two kinds of open and closeds.Open type biological reactor is not efficient manner owing to be affected by the external environment greatlyyer; Compare with the open reactive device, closed has and is easy to guarantee gnotobasis, is easy to control culture condition, be easy to realize advantages such as high-density culture, therefore at present closed bioreactor kind is many, uses also relatively extensively, mainly contains several forms such as tubular type, flat, pillar.
The tower bioreactor of bubbling is that a platform is arranged on the reactor base plate, around the platform guard post and light source are arranged, light source all links to each other with time controller, and the reactor tank body is located on the platform, tank base has a temperature control interlayer, and air intake duct and carbon dioxide pipe enter in the gas container respectively; The mixed gas escape pipe stretches out with strainer from the distribution container and links to each other, and links to each other with the gas distributor that is positioned at tank base by inlet pipe, and higher light-receiving area/volume ratio is arranged, and is difficult for producing the oxygen accumulation.
Patent publication No. CN101613664A discloses a kind of airlift photobioreactor, comprise the intravital inner light source device of reactor tank, inoculation mouth and discharge gate on the reactor tank body wall, and the gauge hatch in the capping at reactor tank body top, the following capping and the temperature controller of venting port and bottom, dispenser device on following capping central through hole, and rely on wherein that a plurality of distribution passages communicate with the inner cavity chamber of reactor tank body, nutrient substance is entered by material hole through feeding tube respectively and distributes in the passage.This invention has realized the uniformly distributing of multimedium in reactor and has mixed, and is applicable to laboratory or industrial scale both culturing microalgae.
Bubbling style and airlift photobioreactor are two the most frequently used in the airtight cultivation of little algae class reactors.Because their simple in structure, easy handlings, and mixture strength and mass-transfer efficiency be all than higher, and fluid type is very clear and definite.But two kinds of reactors are compared, and air lift type is more suitable for little algae and cultivates, because cell density skewness phenomenon can appear in bubbling style reactor in the axial direction, cause the part microalgae cell hungry even dead easily.The membrane type bioreactor of strengthening little algae cultivation is to improve on the airlift reactor basis, and ring-type or crosswise porous gas sparger are replaced with the dead end hollow-fibre membrane.This improvement has effectively reduced bubble diameter, has enlarged the gas-to-liquid contact area, thereby has improved the gas-liquid mass transfer efficient of inside reactor, helps lower concentration CO
2In time be supplied to frustule, and with the O that produces
2In time remove, avoid the photorespiration phenomenon to produce.
We in early stage studies show that (Fan LH, Zhang YT, Cheng LH, Zhang L, TangDS, Chen HL, Optimization of carbon dioxide fixation by Chlorelia vulgariscultivated in a membrane-photobioreactor, Chemical Engineering ﹠amp; Technology, 2007,30 (8): 1094-1099): membrane bioreactor can improve the rate of mass transfer of carbonic acid gas and oxygen greatly, when cell density 15 * 10
7Individual/mL, induction air flow ratio 1.25L/min, red inner light source density 10800 luxs, the length 45cm of film, temperature is in 25 degrees centigrade, and the membrane bioreactor stabilizing carbon dioxide transforms oxygen and exceeds 0.95 times and 0.86 times respectively than common bio-reactor.Carbonic acid gas is by crystallized ability and the air inlet of algae in bio-reactor, and illumination and membrane module have much relations.
Summary of the invention
The invention provides a kind of simple in structure, easy and simple to handle, stable performance is provided, have the membrane type bioreactor of cultivating than the little algae of the reinforcement of bigger serface and light transfer efficiency, this reactor adopts membrane module, make gas distribution even, can use separately, perhaps be used for little algae amplification culture with the logotype of runway pond.
A kind of membrane type bioreactor of strengthening little algae cultivation comprises vertically disposed inner sleeve and outer sleeve on outer light source, base plate and the base plate; Described outer sleeve is positioned at the outside of inner sleeve and inner sleeve is surrounded; The outer sleeve top is provided with horizontally disposed transparent lamina tecti, and the top is provided with some air outlets, and the bottom is provided with discharge port; Described inner sleeve bottom is provided with some exchange holes, described base plate is provided with the inlet mouth that is surrounded by inner sleeve, wherein, described air outlet is positioned at top, inner sleeve top, described base plate is provided with and is positioned at the membrane module that the inner sleeve inboard, upright is provided with, and the bottom margin of membrane module and base plate sealing and fixing are also surrounded inlet mouth.
In order to satisfy the needs of multiple occasion, can on base plate, be provided with the opening for feed that is surrounded by inner sleeve, opening for feed is connected with the feed-pipe of outside, and feed-pipe is provided with trip switch; The exchange hole outside is provided with latch plate, and the inner sleeve outer wall of exchange hole downside and both sides is provided with the chute that cooperates with latch plate, and latch plate can slide up and down along the both sides chute, and when moving on the latch plate, exchange hole is open-minded, exchange hole sealing when sliding to bottom land under the latch plate.When the membrane type bioreactor of the little algae cultivation of above-mentioned reinforcement uses separately,, inner sleeve and outer sleeve are interconnected by exchange hole moving on the latch plate.During operation, close feed-pipe and discharge nozzle valve, ventilate by inlet mouth, utilize gas expansion to provide energy drives liquid to circulate, gas enters membrane module, disperses to enter algae liquid from fenestra, prolong air or carbon dioxide gas mixture and liquid phase duration of contact, promote the absorption of carbonic acid gas, effectively improve the utilization ratio of carbonic acid gas, reduce the inhibition of the oxygen accumulation cell growth of photosynthesis generation.The excessive outer sleeve that enters of inner sleeve algae liquid, the suitable inner sleeve wall of algae liquid by movable span, enters inner sleeve in the bottom again to dirty in the outer sleeve under action of gravity, thus the circulation between inside reactor realization inside and outside sleeve.It is fashionable that the membrane type bioreactor of cultivating when the little algae of above-mentioned reinforcement is used for outdoor amplification field, general and runway pond logotype, during logotype latch plate moved down exchange hole is sealed, separate inside and outside sleeve, algae liquid enters reactor by opening for feed by the runway pond, and inlet mouth connects membrane module and constantly bloats bubble, makes inner sleeve algae liquid be spilled over to outer sleeve, overflow algae liquid and be circulated to the runway pond, effectively amplified the culture area of algae liquid by discharge port.
Membrane module can be selected the cylindrical membrane assembly for use, and the cylindrical membrane assembly is made up of the airtight hollow-fibre membrane of vertically disposed top film port, and the top height of membrane module is lower than the top height of inner sleeve.Membrane module also can be selected other shapes that can realize said function for use, can adjust according to actual needs.Adopt membrane module top film port airtight, prevent that gas from directly discharging from the film port, guarantee that gas only passes through from fenestra, fully disperse gas, gas contact with algae liquid more fully, effectively anti-block accumulates.Experiment showed, with the air-blowing of film port and compare that the fenestra air-blowing can significantly improve gas-liquid mass transfer efficient, and when the total area of film constant, when increasing the tubular fibre film length, help gas-liquid mixed and improve the carbon dioxide fixation rate.
Inner sleeve and outer sleeve generally adopt the transparent organic glass material, tool good light transmittance and wear resistance.Outer sleeve and inner sleeve are fixed by flange and base plate respectively, for ease of the fixing flange face that projection can be set in the position of base plate correspondence.For preventing algae liquid seepage in the reactor operation, between flange bottom surface and base plate, be provided with the rubber cradle or the O type circle that have screw, the end face of rubber cradle and bottom surface were provided with the ring seal striped of projection when installation had the rubber cradle of screw, and the bottom surface of flange and the end face of base plate are respectively equipped with and seal the annular seal groove that striped matches.The number of sealing striped can be determined according to actual stopping property needs.Base plate requires the tool certain thickness, can support the weight of inside and outside sleeve, algae liquid and membrane module.
Lamina tecti is a transparent material, generally selects transparent pmma material for use, can accept natural lighting; Outer light source is made up of the fluorescent tube bulb that plurality of vertical is provided with, and fluorescent tube bulb is evenly distributed on fixing of outer sleeve all around and by support.The number of luminescent lamp, length and installation site are generally determined according to actual needs, for example, can adopt the luminescent lamp of 4～8 30W vertically to be distributed in reactor outward flange 10cm place, guarantee can both obtain uniform illumination at reactor in all directions.
The air outlet outside is provided with baffle plate, and the top of baffle plate and outer sleeve outer wall are fixed, and turns up in the baffle plate bottom, and the bottom edge of baffle plate is lower than the bottom edge of air outlet, can prevent effectively that foreign matter such as extraneous dust from entering internal system.The number of air outlet can be determined according to actual needs, generally is evenly distributed on the top of outer sleeve.The shape of air outlet also can determine not have strict requirement according to actual needs.
Discharge port is connected with external discharge nozzle, and discharge nozzle is provided with thief hole, and thief hole is connected with external stopple coupon, and the thief hole diameter is less than the discharge port diameter.Reactor isolated operation or during with the logotype of runway pond was handled after for some time, when needing the experimental data of testing inner algae liquid, can open thief hole, and the algae liquid sample that takes a morsel is tested.For ease of the conveying of algae liquid generally opening for feed be set to equate with the diameter of discharge port, be generally 15～30mm; The diameter of thief hole is generally 5～10mm.
Inlet mouth is a circular port, inlet mouth place sealing and fixing has transfer tube, the bottom equal diameters of transfer tube top internal diameter and membrane module and mutual sealing and fixing, transfer tube the other end diameter is fixed less than its top end diameter and with external inlet pipe, so more helps entering more uniformly in the fenestra of membrane module by the gas of inlet mouth input.In the actual application, general direct transfer tube is sealed by glue with base plate is tightly connected, and then the tip edge of membrane module bottom and transfer tube is fixed by bolt seal.
For the ease of the circulation of algae liquid between outer sleeve and the inner sleeve, generally the difference of altitude between outer sleeve and the inner sleeve top is set at 30～50cm.The too little algae liquid that causes easily of difference of altitude leaks out outer sleeve by the air outlet, and causes the loss of algae liquid; Difference of altitude then causes the waste of material of outer sleeve too greatly, increases the manufacturing cost of reactor.
Beneficial effect of the present invention is embodied in:
(1) employing of membrane module among the present invention has improved the carbon dioxide fixation ability of membrane type bioreactor, and the contact area between gas phase and the solid phase is increased, and has improved gas-liquid mass transfer speed; Membrane module top film port is airtight, prevents that gas from directly discharging from the film port, guarantees that gas only passes through from fenestra, fully disperses gas, makes gas more fully contact algae liquid, effectively anti-block accumulation;
(2) the membrane type bioreactor of the little algae cultivation of the reinforcement among the present invention is simple in structure, is convenient to operation, and range of application is wider, can use separately, also can be used for the little algae amplification culture with the logotype of runway pond.
Description of drawings
Fig. 1 is the structural representation of the membrane type bioreactor of the little algae cultivation of reinforcement of the present invention;
Fig. 2 is the partial enlarged drawing at the exchange hole place of the membrane type bioreactor that the little algae of reinforcement is cultivated among Fig. 1;
Fig. 3 is the partial enlarged drawing at the C place of the membrane type bioreactor that the little algae of reinforcement is cultivated among Fig. 1;
Fig. 4 is the vertical view of the rubber cradle of the membrane type bioreactor that the little algae of reinforcement is cultivated among Fig. 1;
Fig. 5 is the sectional view along the A-A face of strengthening the membrane type bioreactor of little algae cultivation among Fig. 5;
Fig. 6 is the vertical view of the base plate of the membrane type bioreactor that the little algae of reinforcement is cultivated among Fig. 1;
Fig. 7 is the sectional view along B-B of strengthening the membrane type bioreactor of little algae cultivation among Fig. 6;
Fig. 8 for the membrane type bioreactor strengthening little algae among Fig. 1 and cultivate be used for being communicated with the runway pond time structural representation.
Embodiment
As depicted in figs. 1 and 2, a kind of strengthen that little algae cultivates the membrane type bioreactor, comprise: the inner sleeve 4 and the outer sleeve 3 that vertically are provided with on outer light source 2, horizontally disposed base plate 8 and the base plate 8, for the ease of the circulation of algae liquid between outer sleeve 3 and the inner sleeve 4, the difference of altitude between outer sleeve 3 and inner sleeve 4 tops is set at 40cm.Outer sleeve 3 is positioned at the outside of inner sleeve 4 and inner sleeve 4 is surrounded, outer sleeve 3 tops are provided with horizontally disposed transparent lamina tecti 1, outer sleeve 3 tops are provided with air outlet 20, air outlet 20 is positioned at inner sleeve 4 vertical tops, 20 outsides, air outlet are provided with baffle plate 12, outer sleeve 3 bottoms are provided with discharge port, and discharge port is connected with external discharge nozzle 7, and discharge nozzle 7 is provided with stopple coupon 6; Inner sleeve 4 bottoms are provided with exchange hole 14, and exchange hole 14 outsides are provided with latch plate 10, and base plate 8 is provided with inlet mouth 9 and the opening for feed that is surrounded by inner sleeve 4, and opening for feed links to each other with extraneous feed-pipe 13, and base plate 8 vertically is provided with the membrane module 5 that is positioned at inner sleeve 4 inboards.
Shown in Fig. 3～7, outer sleeve 3 and inner sleeve 4 utilize bolt 11 fixing with base plate 8 by flange 16 respectively, for ease of fixing, the flange face of projection are set in the position of base plate 8 correspondences, are used for flange 16 fixing.For preventing algae liquid seepage in the reactor operation, between flange 16 bottom surfaces and base plate 8, be provided with the rubber cradle 15 that has screw 24, the end face of rubber cradle 15 and bottom surface are provided with the ring seal striped 25 of projection, as shown in Figure 4 and Figure 5.The end face of the bottom surface of flange 16 and base plate 8 is respectively equipped with and seals the annular seal groove 26 that striped 25 matches.The number of sealing striped 25 can determine that base plate requires the tool certain thickness according to actual stopping property needs, can support the weight of inside and outside sleeve, algae liquid and membrane module, as shown in Figure 6 and Figure 7.
As shown in Figure 1, air outlet 20 on the outer sleeve 3 is four square openings that size is 50mm * 50mm, be evenly distributed on outer sleeve 3 tops, 20 outsides, air outlet are provided with baffle plate 12, the top of baffle plate 12 and outer sleeve 3 are fixing, baffle plate 12 bottoms 45 degree that turn up, the bottom edge of baffle plate 12 is lower than the bottom edge of air outlet, can prevent effectively that foreign matter such as extraneous dust from entering internal system.The number of air outlet 20 and shape also can be adjusted according to actual needs.The discharge port of outer sleeve 3 bottoms is connected with external discharge nozzle 7, and discharge nozzle 7 diameters are 20mm, and discharge nozzle 7 is provided with thief hole, and thief hole is connected with external stopple coupon 6, and the stopple coupon diameter is 10mm.During the reactor operation, handle after for some time, when needing the experimental data of testing inner algae liquid, can open stopple coupon 6, the algae liquid sample that takes a morsel is tested.
As shown in Figure 1, inlet mouth 9 on the base plate is trapezoidal circular port, inlet mouth 9 place's sealing and fixing have transfer tube 21, the bottom equal diameters of transfer tube 21 tops and membrane module 5 and interfix by bolt seal, transfer tube 21 passes through the glass cement sealing and fixing with inlet mouth 9, transfer tube 21 the other end diameters are fixed less than its top end diameter and with external inlet pipe, so more help entering more uniformly in the fenestra of membrane module 5 by the gas of inlet mouth 9 inputs.
As Fig. 1, shown in Figure 2, the exchange hole 14 that it is 40mm that inner sleeve 4 bottoms evenly are provided with 4 diameters, exchange hole 14 outsides are provided with quadrate latch plate 10, and the area of latch plate 10 is greater than the area of exchange hole, and the inner sleeve outer wall of exchange hole 14 downsides and both sides is provided with the chute that cooperates with latch plate 10, latch plate 10 can slide up and down along the both sides chute, exchange hole 14 is open-minded when moving on the latch plate 10, and when latch plate 10 was displaced downwardly to bottom land, exchange hole 14 was sealed.
When the membrane type bioreactor of the little algae cultivation of above-mentioned reinforcement uses separately, moving on the latch plate 10, inner sleeve 4 and outer sleeve 3 are interconnected by exchange hole 14, during operation, close the valve of feed-pipe 13 and discharge nozzle 7, by inlet mouth 9 ventilations, utilize gas expansion to provide energy drives liquid to circulate, gas enters membrane module 5, disperses to enter algae liquid from fenestra, prolongs air or carbon dioxide gas mixture and liquid phase duration of contact, promote the absorption of carbonic acid gas, effectively improve the utilization ratio of carbonic acid gas, reduce the inhibition of the oxygen accumulation cell growth of photosynthesis generation, the excessive outer sleeve 3 that enters of inner sleeve 4 algae liquid, under action of gravity algae liquid along inner sleeve wall to dirty, by movable span 14, enter inner sleeve 4 in the bottom again, thus the circulation between inside reactor realization inside and outside sleeve.
As shown in Figure 8, it is fashionable that the membrane type bioreactor of cultivating when the little algae of above-mentioned reinforcement is used for outdoor amplification field, general and runway pond 17 logotypes, latch plate 10 moves to the chute bottom land with exchange hole 14 sealings during logotype, separate inside and outside sleeve, algae liquid in the runway pond is transported to feed-pipe 13 by recycle pump 22, enter reactor by feed-pipe 13 by runway pond 17, inlet mouth 9 connects membrane module 5 and constantly bloats bubble, make inner sleeve 4 algae liquid be spilled over to outer sleeve 3, overflow algae liquid and be circulated to the runway pond, effectively amplified the culture area of algae liquid by discharge nozzle 7;
Even for the thorough mixing of the algae liquid that keeps the runway pond, motor-driven impeller 23 can be installed in the runway pond.Algae liquid flow direction takes back roadside siphunculus 24 and forms shunting with feed-pipe 13 shown in 8 in the runway pond on the recycle pump 22, by regulating the variable valve on the bypass pipe 24, can regulate the flow of the algae liquid that enters reactor.Through overregulating, make the flow of feed-pipe 13 identical with the flow of discharge nozzle 7, reach mass flow equilibrated purpose in the reactor.
Claims (10)
1. strengthen the membrane type bioreactor that little algae is cultivated for one kind, comprise vertically disposed inner sleeve and outer sleeve on outer light source, base plate and the base plate; Described outer sleeve top is provided with horizontally disposed transparent lamina tecti, and the top is provided with some air outlets, and the bottom is provided with discharge port; Described inner sleeve bottom is provided with some exchange holes; Described base plate is provided with the inlet mouth that is surrounded by inner sleeve, it is characterized in that, described air outlet is positioned at top, inner sleeve top; Described base plate is provided with the membrane module of the vertical setting that is positioned at the inner sleeve inboard, and the bottom margin of membrane module and base plate sealing and fixing are also surrounded inlet mouth.
2. the membrane type bioreactor that the little algae of reinforcement according to claim 1 is cultivated is characterized in that described base plate is provided with the opening for feed that is surrounded by inner sleeve, and opening for feed is connected with the feed-pipe of outside, and feed-pipe is provided with trip switch; The described exchange hole outside is provided with latch plate, and the inner sleeve outer wall of exchange hole downside and both sides is provided with the chute that cooperates with latch plate, and latch plate can slide up and down along the both sides chute.
3. the membrane type bioreactor that the little algae of reinforcement according to claim 1 and 2 is cultivated, it is characterized in that, described membrane module is cylindrical, is made up of the vertically disposed top airtight hollow-fibre membrane of fenestra, and the top height of membrane module is lower than the top height of inner sleeve.
4. the membrane type bioreactor that the little algae of reinforcement according to claim 3 is cultivated, it is characterized in that, described inlet mouth is a circular port, inlet mouth place sealing and fixing has transfer tube, the bottom equal diameters of transfer tube top internal diameter and membrane module and mutual sealing and fixing, transfer tube bottom diameter is fixed less than its top end diameter and with external inlet pipe; Transfer tube downside and base plate are fixed.
5. the membrane type bioreactor that the little algae of reinforcement according to claim 1 and 2 is cultivated, it is characterized in that, the outside, described air outlet is provided with baffle plate, and the top of baffle plate and outer sleeve outer wall are fixed and be higher than the air outlet height, and turn up in the baffle plate bottom and bottom edge is lower than the bottom edge of air outlet.
6. the membrane type bioreactor that the little algae of reinforcement according to claim 1 and 2 is cultivated, it is characterized in that, described outer sleeve and inner sleeve are fixed by flange and base plate respectively, be provided with the rubber cradle that has screw between flange bottom surface and the base plate, the end face of rubber cradle and bottom surface are provided with the ring seal striped of projection, and the bottom surface of flange and the end face of base plate are respectively equipped with and seal the annular seal groove that striped matches.
7. the membrane type bioreactor that the little algae of reinforcement according to claim 1 and 2 is cultivated is characterized in that described outer light source is made up of the fluorescent tube bulb that plurality of vertical is provided with, and fluorescent tube bulb is evenly distributed on fixing of outer sleeve all around and by support.
8. the membrane type bioreactor that the little algae of reinforcement according to claim 1 and 2 is cultivated, it is characterized in that described discharge port is connected with external discharge nozzle, discharge nozzle is provided with thief hole, thief hole is connected with external stopple coupon, and the thief hole diameter is less than the discharge port diameter.
9. the membrane type bioreactor that the little algae of reinforcement according to claim 8 is cultivated is characterized in that the equal diameters of described opening for feed and discharge port is 15～30mm; Described thief hole diameter is 5～10mm.
10. the membrane type bioreactor that the little algae of reinforcement according to claim 1 and 2 is cultivated is characterized in that the difference of altitude between described outer sleeve and the inner sleeve top is 30～50cm.
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| CN102304462A (en) * | 2011-08-29 | 2012-01-04 | 暨南大学 | Raceway pond photobiological reactor for implementing microalgae large-scale culture |
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| CN102586078A (en) * | 2012-01-18 | 2012-07-18 | 杭州鑫伟低碳技术研发有限公司 | Microalgae cultivation system and method |
| CN102776120A (en) * | 2012-08-03 | 2012-11-14 | 北京理工大学 | Self-circulating microorganism culture device with oxygen provided by hollow fiber membrane, and culture method |
| CN102816689A (en) * | 2012-08-03 | 2012-12-12 | 北京理工大学 | Flat membrane oxygen supply self-circulation microbial culture device and method |
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| CN102382755A (en) * | 2011-07-28 | 2012-03-21 | 中国科学院青岛生物能源与过程研究所 | Microalgae culturing device and microalgae culturing method |
| CN102304462B (en) * | 2011-08-29 | 2013-06-05 | 暨南大学 | Raceway pond photobiological reactor for implementing microalgae large-scale culture |
| CN102304462A (en) * | 2011-08-29 | 2012-01-04 | 暨南大学 | Raceway pond photobiological reactor for implementing microalgae large-scale culture |
| CN102586078A (en) * | 2012-01-18 | 2012-07-18 | 杭州鑫伟低碳技术研发有限公司 | Microalgae cultivation system and method |
| CN102776120B (en) * | 2012-08-03 | 2013-10-16 | 北京理工大学 | Self-circulating microorganism culture device with oxygen provided by hollow fiber membrane, and culture method |
| CN102816689A (en) * | 2012-08-03 | 2012-12-12 | 北京理工大学 | Flat membrane oxygen supply self-circulation microbial culture device and method |
| CN102816689B (en) * | 2012-08-03 | 2013-10-16 | 北京理工大学 | Device and method for flat membrane oxygen-supply self-circulation-type microbial culture |
| CN102776120A (en) * | 2012-08-03 | 2012-11-14 | 北京理工大学 | Self-circulating microorganism culture device with oxygen provided by hollow fiber membrane, and culture method |
| CN102899239A (en) * | 2012-09-28 | 2013-01-30 | 重庆大学 | Dispersion optical-fiber type photo-bioreactor with inserted spiral band |
| CN104774750A (en) * | 2015-04-24 | 2015-07-15 | 亿利资源集团有限公司 | Photoreactor for culturing microalgae |
| CN107709537A (en) * | 2015-07-01 | 2018-02-16 | 纳尔逊曼德拉大学 | The production method and equipment of microalgae |
| CN107709537B (en) * | 2015-07-01 | 2022-03-25 | 纳尔逊曼德拉大学 | Microalgae production method and equipment |
| CN105505756A (en) * | 2015-12-24 | 2016-04-20 | 青岛理工大学 | For high CO2Breeding device for microalgae with tolerance and fixed rate |
| CN105961303A (en) * | 2016-06-03 | 2016-09-28 | 山东大学 | Fish-bacterium-algae-symbiotic ecological breeding system and running method thereof |
| CN105961303B (en) * | 2016-06-03 | 2019-02-12 | 山东大学 | A fish-bacteria-algae symbiotic ecological breeding system and its operation method |
| CN115159771A (en) * | 2022-06-16 | 2022-10-11 | 科盛环保科技股份有限公司 | Water environment restoration device and process |
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