CN216260673U - A microfluidic structure and microfluidic chip - Google Patents

Abstract

The present application provides a microfluidic structure and a microfluidic chip. The microfluidic structure includes: a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a coaxial flow heterogeneous reaction unit; a continuous outer triangular expansion focusing unit; The unit includes: a continuous liquid phase inlet, a continuous outer triangular expansion focusing flow channel and a continuous liquid phase flow channel; the active valve quantitative uniform control unit includes: a built-in valve plug in the flow channel, a gas phase inlet, a gas phase channel and a gas buffer chamber; The inner wall of the continuous liquid phase flow channel is provided with a built-in valve plug; the coaxial flow heterogeneous reaction unit includes: a reaction liquid phase inlet, a reaction liquid phase flow channel and a mixed liquid phase flow channel. The present application solves the technical problem of how to design a microfluidic device and operation process, so that it can realize fast and accurate quantitative control for accurate and efficient heterogeneous reaction on the basis of generating highly dispersed droplets and particles.

Description

A microfluidic structure and microfluidic chip

technical field

The present application relates to the technical field of microfluidics, and in particular, to a microfluidic channel structure and a microfluidic chip.

Background technique

With the development of science and technology, more and more fields (energy, immunity, biochemistry, etc.) require the use of miniaturized reaction methods for highly dispersed, micro-precision operations. Microfluidic technology can realize many difficult-to-complete micro-processing and micro-operations. received extensive attention. Microfluidics is the use of microchannels and devices to manipulate tiny particles (or samples) that cannot be achieved by some conventional methods. It can integrate biological detection, a series of biochemical reactions and various sample preparations into tiny chips for special operations, and has broad application prospects in many fields.

At present, the conventional preparation process of droplets or microsphere particles is mainly through the mechanical stirring method on a large scale, which cannot accurately screen the microsphere particles of a specific particle size, and the particle dispersibility is low, and the droplets participating in the reaction ( Or particles) too much or too little can not guarantee the efficient progress of the reaction. The droplets (or particles) can be uniformly dispersed through a microfluidic system with a special structure, and then an effective and sufficient reaction can be carried out after quantitative control, which can effectively improve the efficiency and the success rate of the experiment.

There are many ways to use microfluidics to generate (or droplets wrapped with particles), the active type requires an external magnetic field and an electric field; the passive type usually uses Dean flow, which requires no energy input, and the device is simple and easy to maintain and small in size. Passive Dean flow has become one of the most effective methods for microfluidic focusing of droplets (or particle-encapsulated droplets) due to its simple and convenient operation, uniformity and high efficiency. Through passive Dean flow focusing, disorderly scattered microspheres and droplets can be focused in the microchannel to form a queue of microspheres and droplets arranged at equal intervals at specific positions. Although the dispersion of droplets (or particles) can be achieved to a certain extent, it requires a certain length of helical curved flow channel to achieve the purpose, and it is difficult to carry out precise quantitative control.

Therefore, how to design a microfluidic device and operation process, so that it can realize fast and accurate quantitative control for accurate and efficient heterogeneous reaction on the basis of generating highly dispersed droplets and particles, has become an urgent problem in the art. one of the problems.

Utility model content

The purpose of this application is to provide a microfluidic channel structure, a microfluidic chip and a heterogeneous reaction method, which are used to solve how to design a microfluidic device and operation process, so that it can generate highly dispersed droplets, particles On the basis of realizing fast and accurate quantitative control, the technical problem of accurate and efficient heterogeneous reaction is carried out.

In order to solve the above problems, the present application provides a micro-channel structure, including: a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit, and a coaxial flow heterogeneous reaction unit;

The continuous outer triangular expansion focusing unit includes: a continuous liquid phase injection port, a continuous outer triangular expansion focusing flow channel and a continuous liquid phase flow channel;

The continuous liquid phase sample inlet is communicated with the liquid inlet end of the continuous outer triangular expansion focusing flow channel, and the liquid inlet end of the continuous liquid phase flow channel is communicated with the liquid outlet end of the continuous outer triangular expansion focusing flow channel;

The active valve quantitative uniform control unit includes: a built-in valve plug in the flow channel, a gas-phase sample inlet, a gas-phase channel and a gas buffer chamber;

The inner wall of the continuous liquid phase flow channel is provided with the built-in valve plug, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the inlet end is communicated with the gas phase injection port, and the gas buffer chamber is communicated with the gas buffer chamber. Corresponding to the built-in valve plug;

The coaxial flow heterogeneous reaction unit includes: a reaction liquid phase inlet, a reaction liquid phase flow channel and a mixed liquid phase flow channel;

The liquid inlet end of the reaction liquid phase flow channel is communicated with the reaction liquid phase inlet, the liquid outlet end is communicated with the liquid inlet end of the mixed liquid phase flow channel, and the mixed liquid phase flow channel is discharged. The end is communicated with the mixed-phase sample outlet; the liquid inlet end of the mixed-phase flow channel is communicated with the continuous liquid-phase flow channel.

Further, the continuous outer triangular expansion focusing flow channel is helical;

The liquid inlet end of the continuous outer triangular expansion focusing flow channel is located in the center of the spiral;

The liquid outlet end of the continuous outer triangular expansion focusing flow channel is located on the outer side of the helical shape.

Further, there is at least one active valve quantitative uniform control unit.

Further, the built-in valve plug is block-shaped;

The built-in valve plug is arranged on the side of the inner wall of the continuous liquid phase flow channel away from the gas buffer chamber.

Further, the materials of the gas buffer chamber and the continuous liquid phase flow channel are all deformable materials, the gas buffer chamber does not deform in a non-inflated state, and the gas buffer chamber expands in an inflated state and is combined with the gas buffer. The abutment of one side of the continuous liquid phase flow channel means that the inner wall of the continuous liquid phase flow channel is fully in contact with the built-in valve plug, thereby realizing the blocking of the continuous liquid phase flow channel.

Further, the cross-sections of the continuous liquid-phase flow channel, the gas-phase channel and the reaction liquid-phase flow channel are all rectangular, and the heights of the various flow channels are uniform, and the heights are all 100 μm˜200 μm.

The application also provides a microfluidic chip, comprising a chip body and the above-mentioned microfluidic channel structure;

The micro-channel structure is arranged in the chip body.

Further, the chip body includes a substrate and a cover;

the micro-channel structure is arranged on the upper surface of the substrate;

The cover plate covers the upper surface of the substrate, and the continuous liquid phase injection port, the gas phase injection port, the reaction liquid phase injection port and the mixed phase injection port all pass through the the cover plate.

Further, it also includes a conveying device and an extraction device;

The delivery device includes a first delivery pump communicated with the continuous liquid phase inlet, a second delivery pump communicated with the gas phase inlet, and a third delivery pump communicated with the reaction liquid phase inlet ;

The extraction device is communicated with the mixed-phase sample outlet.

Compared with the prior art, the advantages of the embodiments of the present application are:

The present application provides a micro-channel structure, comprising: a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a coaxial flow heterogeneous reaction unit; the continuous outer triangular expansion focusing unit includes: a continuous liquid phase inlet a sample port, a continuous outer triangular expansion focusing flow channel and a continuous liquid phase flow channel; the continuous liquid phase injection port is communicated with the liquid inlet end of the continuous outer triangular expansion focusing flow channel, and the continuous liquid phase flow channel liquid inlet end It is connected with the liquid outlet end of the continuous outer triangular expansion focusing flow channel; the active valve quantitative uniform control unit includes: a built-in valve plug in the flow channel, a gas phase injection port, a gas phase channel and a gas buffer chamber; the continuous liquid phase flow The inner wall of the channel is provided with the built-in valve plug, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the inlet end is communicated with the gas phase inlet, and the gas buffer chamber corresponds to the built-in valve plug; The coaxial flow heterogeneous reaction unit comprises: a reaction liquid phase inlet, a reaction liquid phase flow channel and a mixed liquid phase flow channel; the liquid inlet end of the reaction liquid phase flow channel and the reaction liquid phase injection The liquid outlet end is communicated with the liquid inlet end of the mixed liquid phase flow channel, and the liquid outlet end of the mixed liquid phase flow channel is communicated with the mixed phase sample outlet; the liquid inlet end of the mixed liquid phase flow channel The end communicates with the continuous liquid phase flow channel.

The microfluidic channel structure provided in this application includes a continuous outer triangular expansion focusing unit, an active valve quantitative uniform control unit and a coaxial flow heterogeneous reaction unit. The continuous outer triangular expansion focusing unit includes a continuous liquid phase injection port, a continuous The outer triangular expansion focusing flow channel and the continuous liquid phase flow channel, the continuous liquid phase injection port is used to introduce the sample (droplets or particles), and the sample is separated layer by layer through the continuous outer triangular structure of the continuous outer triangular expansion focusing flow channel, so that the The sample forms microspheres of the same size and equidistantly distributed and enters the continuous liquid phase channel. The opening and closing degree of the continuous liquid phase channel is controlled by the active valve quantitative uniform control unit, thereby controlling the flow rate of the microspheres, realizing quantitative control, and finally. Entering the mixed liquid phase flow channel, by introducing the reaction liquid at the reaction liquid phase inlet, and allowing the reaction liquid to enter the mixed liquid phase flow channel through the reaction liquid phase flow channel to be fully mixed with the microspheres, so as to achieve fast and accurate quantitative control. Accurate and efficient heterogeneous reaction, it solves how to design a microfluidic device and operation process, so that it can realize fast and accurate quantitative control on the basis of generating highly dispersed droplets and particles for accurate and efficient heterogeneous reaction. technical problem.

Description of drawings

In order to more clearly illustrate the specific embodiments of the present application or the technical solutions in the prior art, the accompanying drawings required in the description of the specific embodiments or the prior art will be briefly introduced below. The drawings are some embodiments of the present application. For those of ordinary skill in the art, other drawings can also be obtained from these drawings without any creative effort.

1 is a top view of a microfluidic structure provided by an embodiment of the present application;

2 is a top view of a continuous outer triangular expansion focusing flow channel provided by an embodiment of the present application;

Fig. 3 is the control principle diagram of the active valve quantitative uniform control unit in the application embodiment;

FIG. 4 is an overall structural diagram of a microfluidic chip provided by an embodiment of the present application.

The reference numerals are: continuous outer triangular expansion focusing unit 1, active valve quantitative uniform control unit 2, coaxial flow heterogeneous reaction unit 3, continuous liquid phase injection port 4, continuous outer triangular expansion focusing flow channel 5, Continuous liquid phase flow channel 6, built-in valve plug 7, gas phase inlet 8, gas phase channel 9, gas buffer chamber 10, reaction liquid phase inlet 11, reaction liquid phase flow channel 12, mixed liquid phase flow channel 13, substrate 14. Cover plate 15 , first delivery pump 16 , second delivery pump 17 , third delivery pump 18 , extraction device 19 , first flow channel 20 , second flow channel 21 , mixed-phase sample outlet 22 .

Detailed ways

The technical solutions of the present application will be clearly and completely described below with reference to the accompanying drawings. Obviously, the described embodiments are part of the embodiments of the present application, but not all of the embodiments. Based on the embodiments in the present application, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present application.

In the description of this application, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. The indicated orientation or positional relationship is based on the orientation or positional relationship shown in the accompanying drawings, which is only for the convenience of describing the present application and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation or a specific orientation. construction and operation, and therefore should not be construed as limitations on this application. Furthermore, the terms "first", "second", and "third" are used for descriptive purposes only and should not be construed to indicate or imply relative importance.

Unless otherwise expressly specified and limited, the terms "installed", "connected" and "connected" should be understood in a broad sense, for example, it may be a fixed connection, a detachable connection, or an integral connection; it may be a mechanical connection, It can also be an electrical connection; it can be a direct connection, an indirect connection through an intermediate medium, or an internal connection between two components. For those of ordinary skill in the art, the specific meanings of the above terms in this application can be understood in specific situations.

For ease of understanding, please refer to Figures 1 to 3.

The embodiment of the present application provides a micro-channel structure, which is characterized by comprising: a continuous outer triangular expansion focusing unit 1, an active valve quantitative uniform control unit 2, and a coaxial flow heterogeneous reaction unit 3;

The continuous outer triangular expansion focusing unit 1 includes: a continuous liquid phase injection port 4, a continuous outer triangular expansion focusing flow channel 5 and a continuous liquid phase flow channel 6;

The continuous liquid phase sample inlet 4 is communicated with the liquid inlet end of the continuous outer triangular expansion focusing flow channel 5, and the liquid inlet end of the continuous liquid phase flow channel 6 is communicated with the liquid outlet end of the continuous outer triangular expansion focusing flow channel 5;

The active valve quantitative uniform control unit 2 includes: a built-in valve plug 7 in the flow channel, a gas-phase sample inlet 8, a gas-phase channel 9 and a gas buffer chamber 10;

The inner wall of the continuous liquid phase flow channel 6 is provided with a built-in valve plug 7, the gas outlet end of the gas phase channel 9 is communicated with the gas buffer chamber 10, the inlet end is communicated with the gas phase inlet 8, and the gas buffer chamber 10 corresponds to the built-in valve plug;

The coaxial flow heterogeneous reaction unit 3 includes: a reaction liquid phase inlet 11, a reaction liquid phase flow channel 12 and a mixed liquid phase flow channel 13;

The liquid inlet end of the reaction liquid phase flow channel 12 is communicated with the reaction liquid phase inlet 11, the liquid outlet end is communicated with the liquid inlet end of the mixed liquid phase flow channel 13, and the liquid outlet end of the mixed liquid phase flow channel 13 is connected with the mixed liquid phase flow channel 13. The sample outlet 22 is communicated; the liquid inlet end of the mixed liquid phase flow channel 13 is communicated with the continuous liquid phase flow channel 6 .

It should be noted that the inner sidewall of the continuous outer triangular expansion focusing flow channel 5 is a continuous zigzag shape, and the top view angle is similar to a plurality of continuous triangles, and the triangles are preferably equilateral triangles, so as to achieve equidistant dispersion of the samples, forming For more uniform microsphere particles, the narrowest part of the continuous outer triangular expansion focusing flow channel 5 is the same as the cross section of the continuous liquid phase flow channel 6, so that the docking can be carried out. The bottom surface of the continuous outer triangular expansion focusing flow channel 5 is preferably a smooth wall surface, In order to facilitate the flow of the sample, the wall surface of the corresponding continuous liquid phase flow channel 6 may also preferably be a smooth wall surface.

Preferably, in order to avoid direct contact between the gas phase channel 9 and the wall surface of the continuous liquid phase flow channel 6 resulting in excessive pressure at the contact point of the wall surface of the continuous liquid phase flow channel 6 and damage to the wall surface, a gas buffer chamber 10 is provided to separate the gas phase channel 9 and the liquid phase flow channel. The buffer chamber keeps a certain distance from the wall of the liquid flow channel.

The coaxial flow heterogeneous reaction unit 3 is composed of the highly dispersed liquid droplets (or microspheres) continuous liquid phase flow channel 6 after quantitative and uniform control by the active valve, the reaction liquid phase sampling flow channel and the mixed liquid phase flow channel. Road 13 composition. Wherein, the number of the reaction liquid phase inlet 11 and the reaction liquid phase flow channel 12 is preferably two, and one reaction liquid phase inlet 11 and one reaction liquid phase flow channel 12 are matched, so that the two reaction liquids can be simultaneously Add to make the function of the micro-channel structure stronger, preferably, the reaction liquid-phase flow channel 12 includes a first flow channel 20 and a second flow channel 21, and one end of the first flow channel 20 is communicated with one end of the second flow channel 21, The other end of the first flow channel 20 is communicated with the reaction liquid phase inlet 11, the other end of the second flow channel 21 is communicated with the liquid inlet end of the mixed liquid phase flow channel 13, and the first flow channel 20 and the second flow channel 21 are the same. The first flow channel 20 runs horizontally, and the direction of the second flow channel 21 and the first flow channel 20 form an included angle of 45°. The diameter of the mixed liquid-phase flow channel 13 is larger than the diameter of the continuous liquid-phase flow channel 6 and the diameter of the reaction liquid-phase flow channel 12 , so that the mixed liquid-phase flow channel 13 can better accommodate the microspheres and various reaction liquids at the same time.

The microfluidic channel structure provided in this application includes a continuous outer triangular expansion focusing unit 1, an active valve quantitative uniform control unit 2 and a coaxial flow heterogeneous reaction unit 3, and the continuous outer triangular expansion focusing unit 1 includes a continuous liquid phase feeding unit. Sample port 4, continuous outer triangular expansion focusing flow channel 5 and continuous liquid phase flow channel 6, the continuous liquid phase injection port 4 is used to introduce samples (droplets or particles), and the sample passes through the continuous outer triangular expansion focusing flow channel 5. The outer triangular structure is separated layer by layer, so that the sample forms microspheres of the same size and equidistantly distributed and enters the continuous liquid phase channel. The flow rate of the ball is quantitatively controlled, and finally enters the mixed liquid phase flow channel 13. By introducing the reaction liquid at the reaction liquid phase inlet 11, the reaction liquid enters the mixed liquid phase flow channel 13 through the reaction liquid phase flow channel 12. It is fully mixed with microspheres to achieve fast and accurate quantitative control for precise and efficient heterogeneous reactions. The technical problem of realizing fast and accurate quantitative control for precise and efficient heterogeneous reaction.

As a further improvement, the continuous outer triangular expansion focusing flow channel 5 of the micro-channel structure provided in the embodiment of the present application is helical;

The liquid inlet end of the continuous outer triangular expansion focusing flow channel 5 is located in the center of the spiral;

The liquid outlet end of the continuous outer triangular expansion focusing flow channel 5 is located on the outer side of the spiral.

Specifically, the use of a helical structure is beneficial to reduce the maximum length of the continuous outer triangular expansion flow channel in the case of a small occupied area, so that the separation and dispersion effect of the sample is better.

As a further improvement, there is at least one active valve quantitative uniform control unit 2 of the micro-channel structure provided in the embodiment of the present application. Preferably, there are two. By setting two active emission quantitative uniform control units, it is beneficial for them to control the flow rate in the continuous liquid phase flow channel 6 by stages, so that the flow rate in the continuous liquid phase flow channel 6 is gradually reduced, so that there are It is beneficial to control its final flow more accurately.

As a further improvement, the built-in valve plug 7 provided in the embodiment of the present application is block-shaped;

The built-in valve plug 7 is disposed on the side of the inner wall of the continuous liquid phase flow channel 6 away from the gas buffer chamber 10 . Preferably, the built-in valve plug 7 is in the shape of a rectangular parallelepiped.

As a further improvement, the materials of the gas buffer chamber 10 and the continuous liquid phase flow channel 6 provided in the embodiment of the present application are all deformable materials, the gas buffer chamber 10 does not deform in a non-inflated state, and the gas buffer chamber 10 is inflated. It expands and abuts one side of the continuous liquid phase flow channel 6 , so that the inner wall of the continuous liquid phase flow channel 6 is fully contacted with the built-in valve plug 7 , thereby realizing the blocking of the continuous liquid phase flow channel 6 . Specifically, the gas source in the gas phase channel 9 comes from the gas introduced by the gas phase injection port 8, and the gas phase injection port 8 can be connected to an external device such as an air pump.

As a further improvement, the continuous liquid phase flow channel 6, the gas phase channel 9 and the reaction liquid phase flow channel 12 provided in the embodiments of the present application are all rectangular in cross section, and the heights of the various flow channels are uniform, and the heights are all 100 μm～ 200μm.

Preferably, the total length of the continuous outer triangular expansion focusing flow channel 5 is 200 mm to 2000 mm; the width of the continuous outer triangular expansion focusing flow channel 5 is 100 μm to 200 μm; The radius of curvature of the innermost flow channel of the continuous outer triangular expansion focusing flow channel 5 is 20 mm to 50 mm.

Preferably, the width of the triangular wall and the lower smooth wall of the continuous outer triangular expansion focusing flow channel 5 is 100 μm～300 μm, the wall structure of the triangular flow channel is an equilateral triangle and the side length is 50 μm～200 μm; the total length of the flow channel is 500 mm～300 μm. 5000mm, the distance between two adjacent vortex focusing curves is 200μm～500μm, and the innermost channel curvature radius is 10mm～100mm;

Preferably, the distance between the gas buffer chamber 10 and the liquid phase flow channel wall is 50 μm˜200 μm;

Preferably, the mixed liquid-phase flow channel 13 wraps the end of the continuous liquid-phase flow channel 6 and the reaction liquid-phase sampling flow channel to form a coaxial flow, the reaction liquid-phase sampling flow channel has a width of 50 μm to 200 μm, and the continuous liquid-phase flow channel 6 The width of the end is 100 μm～200 μm, and the width of the mixed liquid phase flow channel 13 is 200 μm～400 μm;

Preferably, the width of the continuous gas phase channel 9 is 40 μm˜120 μm.

Referring to FIGS. 1 to 4 , the present application also provides a microfluidic chip, which is characterized in that it includes a chip body and the microfluidic channel structure in the above embodiment; the microfluidic channel structure is disposed in the chip body.

Preferably, the material of the chip body is preferably transparent PDMS as the chip material, which can be observed, photographed and recorded directly with a microscope.

As a further improvement, the chip body of the microfluidic chip provided in the embodiment of the present application includes a substrate 14 and a cover plate 15; the microfluidic channel structure is arranged on the upper surface of the substrate 14; the cover plate 15 covers the upper surface of the substrate 14, And the continuous liquid phase inlet 4 , the gas phase inlet 8 , the reaction liquid phase inlet 11 and the mixed phase outlet 22 all pass through the cover plate 15 .

As a further improvement, the microfluidic chip provided in the embodiment of the present application further includes a delivery device and an extraction device 19; the delivery device includes a first delivery pump 16 communicated with the continuous liquid phase inlet 4, and a gas phase inlet 8 The second delivery pump 17 in communication, the third delivery pump 18 in communication with the reaction liquid phase sample inlet 11 ; the extraction device 19 is in communication with the mixed phase sample outlet 22 . Wherein, the first delivery pump 16 is used to deliver the sample into the continuous liquid phase inlet 4; the second delivery pump 17 is used to deliver the origin into the gas phase inlet 8; the third delivery pump 18 is used to deliver the reaction liquid into the reaction liquid phase The injection port 11, specifically, because both the reaction liquid phase injection port 11 and the reaction liquid phase flow channel 12 are preferably two, one reaction liquid phase injection port 11 and one reaction liquid phase flow channel 12 are matched, so the first There may preferably be two three delivery pumps 18 , and each third delivery pump 18 may be communicated with one reaction liquid phase inlet 11 respectively, and is used to deliver the same or different reaction liquids to the corresponding reaction liquid phase inlets 11 . .

The microfluidic chip provided by this application has the following advantages:

1. Miniaturization of device structure. The entire chip device has a small area and a large specific surface area. The coordinated operation of the delivery device and the extraction device 19 enables high throughput.

2. High dispersion stability. The continuous outer triangular expansion focusing flow channel 5 is more conducive to passive Dean flow inertial focusing, which increases the particle dispersion stability.

3. Precise quantitative control. The microspheres passing through the focusing curve have a uniform height and spacing. By adjusting the pneumatic pump, the number of particles (or droplets) in the continuous liquid phase can be quickly and accurately controlled to achieve precise and controllable quantitative control.

4. Easy to observe. The device can use transparent PDMS as the chip material, and can directly use a microscope to observe, photograph and record.

5, to adapt to a wide range of fields. Due to the separation between the gas phase channel 9 and the liquid phase channel, the gas will not react to the particles in the reaction liquid, and is suitable for many heterogeneous reactions.

6. Low cost. During the operation, only less particles and reaction liquid are needed to achieve a uniform and sufficient reaction that is difficult to achieve in conventional operations.

The present application also provides a heterogeneous reaction method, which is applied to the microfluidic channel structure described in the above embodiment, and is characterized in that, it includes the steps:

S1. Disperse the microsphere suspension uniformly and stably through the continuous outer triangular expansion focusing flow channel 5 and flow into the continuous liquid phase channel;

S2, adjusting the opening and closing of the continuous liquid phase channel through the gas phase channel 9 of the quantitative uniform control unit of the active valve, and accurately controlling the number of microspheres in the microsphere suspension flowing into the coaxial flow heterogeneous reaction unit;

S3. The reaction liquid enters the mixed liquid phase flow channel 13 through the reaction liquid phase flow channel 12, and is mixed and reacted with the microsphere suspension in the mixed liquid phase flow channel 13.

The above is the first embodiment provided by the application, and the following is the second embodiment provided by the application, specifically:

The material of the chip body is PDMS, the length of the continuous outer triangular expansion focusing flow channel 5 is 1000mm, the distance between two adjacent vortex focusing flow channels is 150μm, the curvature radius of the innermost flow channel is 20mm, the gas flow channel and the reaction liquid flow channel are The width of 12 is 50 μm, the width of continuous liquid phase flow channel 6 is 100 μm, the width of mixed liquid phase flow channel 13 is 200 μm, the gas buffer chamber 10 and the liquid phase flow channel wall keep a certain distance of 40 μm, and the height of all flow channels is 100 μm. Nitrogen gas was selected as the gas phase, a 50% alcohol solution containing polystyrene microspheres with a particle size of 30 μm was used as the sample solution, and a deionized aqueous solution was used as the reaction sample solution. Fluid is injected into the chip body using a teflon capillary hose, and a second delivery pump 17 is used to control the gas phase fluid. The flow rate of the sample liquid is 30μl/min, the flow rate of the gas phase is 50μl/min, and the flow rate of the reaction liquid phase is 30μl/min. Adjusting the flow rate of the gas phase and the continuous phase liquid phase can obtain the liquid phase with various quantitative microsphere contents. Liquid-fluid mixing completes quantitative heterogeneous reactions.

The above is the second embodiment provided by the application, and the following is the third embodiment provided by the application, specifically:

The material of the chip body is PDMS, the length of the continuous outer triangular expansion focusing channel 5 is 1100mm, the distance between two adjacent vortex focusing channels is 130μm, the curvature radius of the innermost channel is 15mm, the gas-phase channel and the reaction liquid-phase channel are The width of 12 is 60 μm, the width of continuous liquid phase flow channel 6 is 100 μm, the width of mixed liquid phase flow channel 13 is 180 μm, the gas buffer chamber 10 and the liquid phase flow channel wall keep a certain distance of 50 μm, and the height of all flow channels is 100 μm. Nitrogen gas was selected as the gas phase, a modified titanium dioxide solution containing a particle size of 50 μm was used as the sample solution, and a deionized aqueous solution was used as the reaction sample solution. Fluids were injected into the chip using teflon capillary hoses and gas phase fluids were pumped using a second delivery pump 17 . The flow rate of the sample liquid is 20μl/min, the flow rate of the gas phase is 50μl/min, and the flow rate of the reaction liquid phase is 20μl/min. Adjusting the flow rate of the gas phase and the continuous phase liquid phase can obtain the liquid phase with various quantitative microsphere contents. Liquid-fluid mixing completes quantitative heterogeneous reactions.

The above embodiments are only used to illustrate the technical solutions of the present application, but not to limit them; although the present application has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: it can still be used for the foregoing implementations. The technical solutions described in the examples are modified, or some or all of the technical features thereof are equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions depart from the scope of the technical solutions of the embodiments of the present application.

Claims (9)

1. a micro-channel structure, is characterized in that, comprises: continuous outer triangular expansion focusing unit, active valve quantitative uniform control unit and coaxial flow heterogeneous reaction unit; The continuous outer triangular expansion focusing unit includes: a continuous liquid phase injection port, a continuous outer triangular expansion focusing flow channel and a continuous liquid phase flow channel; The continuous liquid phase sample inlet is communicated with the liquid inlet end of the continuous outer triangular expansion focusing flow channel, and the liquid inlet end of the continuous liquid phase flow channel is communicated with the liquid outlet end of the continuous outer triangular expansion focusing flow channel; The active valve quantitative uniform control unit includes: a built-in valve plug, a gas-phase sample inlet, a gas-phase channel and a gas buffer chamber; The inner wall of the continuous liquid phase flow channel is provided with the built-in valve plug, the gas outlet end of the gas phase channel is communicated with the gas buffer chamber, the inlet end is communicated with the gas phase injection port, and the gas buffer chamber is communicated with the gas buffer chamber. Corresponding to the built-in valve plug; The coaxial flow heterogeneous reaction unit includes: a reaction liquid phase inlet, a reaction liquid phase flow channel and a mixed liquid phase flow channel; The liquid inlet end of the reaction liquid phase flow channel is communicated with the reaction liquid phase sample inlet, the liquid outlet end is communicated with the liquid inlet end of the mixed liquid phase flow channel, and the liquid outlet end of the mixed liquid phase flow channel is connected to the liquid inlet end of the mixed liquid phase flow channel. The mixed-phase sample outlet is communicated; the liquid inlet end of the mixed-phase flow channel is communicated with the continuous liquid-phase flow channel. 2. The micro-channel structure according to claim 1, wherein the continuous outer triangular expansion focusing flow channel is helical; The liquid inlet end of the continuous outer triangular expansion focusing flow channel is located in the center of the spiral; The liquid outlet end of the continuous outer triangular expansion focusing flow channel is located at the outer side of the helical shape. 3 . The micro-channel structure according to claim 1 , wherein the active valve has at least one quantitative uniform control unit. 4 . 4. The micro-channel structure according to claim 1, wherein the built-in valve plug is block-shaped; The built-in valve plug is arranged on the side of the inner wall of the continuous liquid phase flow channel away from the gas buffer chamber. 5. The microfluidic structure according to claim 4, characterized in that, The materials of the gas buffer chamber and the continuous liquid phase flow channel are all deformable materials, the gas buffer chamber does not deform in a non-inflated state, and the gas buffer chamber expands in an inflated state and is connected to the continuous flow. One side of the liquid-phase flow channel is in contact with each other, and the inner wall of the continuous liquid-phase flow channel is fully in contact with the built-in valve plug, so as to realize the blocking of the continuous liquid-phase flow channel. 6 . The micro-channel structure according to claim 1 , wherein the cross-sections of the continuous liquid-phase flow channel, the gas-phase channel and the reaction liquid-phase flow channel are all rectangular, and the heights of the various flow channels are uniform, 6 . And the heights are all 100 μm to 200 μm. 7. A microfluidic chip, characterized in that it comprises a chip body and the microfluidic channel structure according to any one of claims 1-6; The micro-channel structure is arranged in the chip body. 8. The microfluidic chip according to claim 7, wherein the chip body comprises a substrate and a cover plate; the micro-channel structure is arranged on the upper surface of the substrate; The cover plate covers the upper surface of the substrate, and the continuous liquid phase injection port, the gas phase injection port, the reaction liquid phase injection port and the mixed phase injection port all pass through the the cover plate. 9. The microfluidic chip according to claim 7, further comprising a conveying device and an extraction device; The delivery device includes a first delivery pump communicated with the continuous liquid phase inlet, a second delivery pump communicated with the gas phase inlet, and a third delivery pump communicated with the reaction liquid phase inlet ; The extraction device is communicated with the mixed-phase sample outlet.

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