CN211190233U - A microfluidic structure and microfluidic chip for quantitative heterogeneous reactions - Google Patents

Abstract

The utility model belongs to the technical field of it is micro-fluidic, especially, relate to a miniflow channel structure and micro-fluidic chip for quantitative heterogeneous reaction. The utility model discloses among the miniflow structure, microballon focus unit forms sharp microballon queue with the microballon focus and keeps equidistant arranging, the initiative valve quantitative control unit carries out the ration dispersion to the microballon that focuses on equidistant range, with the reaction liquid in the heterogeneous reaction unit's of axle flow second liquid phase runner and the third liquid phase runner fully wrap up the microballon of ration dispersion and carry out heterogeneous reaction, realize the quantitative heterogeneous reaction of microballon, the heterogeneous reaction that the unable accurate control of solution microballon quantity leads to is insufficient, inhomogeneous problem.

Description

A microfluidic structure and microfluidic chip for quantitative heterogeneous reactions

technical field

The utility model belongs to the technical field of microfluidics, in particular to a microfluidic channel structure and a microfluidic control chip for quantitative heterogeneous reaction.

Background technique

In recent years, many fields such as biology, chemistry, energy, and environmental protection have increasingly used miniaturized reaction methods for micro-precision operations, and microfluidic technology is one of the most important technical methods. Microfluidic technology utilizes micro-scale channels and devices to manipulate trace amounts of liquids (or samples), and can integrate sample preparation, chemical reactions, and detection into tiny chips for systematic, programmed, and standardized operations.

Conventional microdroplet preparation is currently mainly carried out by mechanical stirring method. This method cannot accurately control the particle size and cannot ensure the effective and sufficient progress of the reaction due to the inability to precisely control the amount of reactants. Microfluidic technology has a very small injection volume, and the reaction is fast, accurate and easy to automate control, especially the characteristics of large specific surface area, shortened operating distance and controllable whole process brought by micro-sized channels, and can control precise and complex liquid flow, etc. Making it a popular alternative to conventional reactions in some fields.

The methods of generating microdroplets using microfluidics can be mainly divided into two categories: active and passive. The active method refers to obtaining micro droplets by applying external forces such as air pressure and electric field to change the flow of the liquid. The passive method controls the flow of the liquid phase by changing the shape of the flow channel of the microchannel and the flow characteristics of the fluid to generate microdroplets, which is simple and convenient to operate and has a low production cost. Passive Dean flow is one of the most effective ways to focus microspheres in microfluidics. Passive Dean flow can focus the chaotically dispersed microspheres in the microchannel into an array of microspheres arranged at equal intervals. It uses the centrifugal force of the fluid in the spiral focusing curved microchannel to induce secondary eddy currents, and exerts a Dean drag force on the microspheres in the liquid phase. At the same time, the microspheres are also subjected to the inertial lift force of the inertial flow, and the microspheres are simultaneously affected by the two forces. Under the combined action, the final fixed position on the flow channel section remains unchanged.

Although the uniformly spaced array of microspheres after passive Dean flow focusing can achieve dispersion to a certain extent, how to achieve precise and quantitative control of the number of microspheres and then fully mix them with the reaction solution for heterogeneous, accurate and efficient reaction has become a problem. One of the problems to be solved urgently in the art.

Utility model content

In view of this, the present application provides a microfluidic channel structure and a microfluidic chip for quantitative heterogeneous reactions, which are used to solve the problem that the prior art cannot accurately and quantitatively control the number of microspheres during heterogeneous reactions. The problem.

The concrete technical scheme of the present utility model is as follows:

A microfluidic channel structure, comprising: a microsphere focusing unit, an active valve quantitative control unit and a coaxial flow heterogeneous reaction unit;

The microsphere focusing unit includes a first liquid phase injection port and a vortex focusing curve, and the first liquid phase injection port is communicated with the first end of the vortex focusing curve;

The active valve quantitative control unit includes a first liquid-phase flow channel, a gas-phase injection port and a gas-phase flow channel, the first liquid-phase flow channel is communicated with the second end of the vortex focusing curve, and the first liquid-phase flow channel is in communication with the second end of the vortex focusing curve. The inner wall of the liquid-phase flow channel is provided with a valve block, the gas-phase injection port is communicated with the first end of the gas-phase flow channel, the gas-phase flow channel is not communicated with the first liquid-phase flow channel, and the gas-phase flow channel is not communicated with the first liquid-phase flow channel. The flow channel is used to adjust the opening and closing of the first liquid-phase flow channel provided with the valve block;

The coaxial flow heterogeneous reaction unit includes a first liquid-phase flow channel, a second liquid-phase flow channel, a third liquid-phase flow channel and a fourth liquid-phase flow channel, the outlet of the second liquid-phase flow channel and the The outlet of the third liquid-phase flow channel is connected to the fourth liquid-phase flow channel and is located outside the first liquid-phase flow channel, and the first liquid-phase flow channel and the fourth liquid-phase flow channel are the same as the first liquid-phase flow channel. axis and the outlet of the first liquid phase flow channel is disposed at the center of the fourth liquid phase flow channel.

Preferably, the valve blocks and the gas-phase flow passages have the same number;

The number of the valve blocks is two or more.

Preferably, the active valve quantitative control unit further includes a gas buffer chamber;

The gas buffer chamber communicates with the second end of the gas-phase flow channel, and the gas-phase flow channel can be opened and closed at the first liquid-phase flow channel provided with the valve block through the gas buffer chamber.

Preferably, the valve block is a cuboid valve block;

The contact surface of the gas buffer chamber and the first liquid phase flow channel is a curved surface in a working state.

Preferably, the cross-sectional shapes of the vortex focusing curve, the first liquid phase flow channel and the gas phase flow channel are the same;

The heights of the vortex focusing curve, the first liquid-phase flow channel and the gas-phase flow channel are the same and are 100 μm˜200 μm.

Preferably, the total length of the vortex focusing curve is 100mm-1000mm;

The width of the vortex focusing curve is 100 μm˜200 μm;

The distance between two adjacent flow channels of the vortex focusing curve is 100 μm˜300 μm;

The radius of curvature of the innermost flow channel of the vortex focusing curve is 20mm˜30mm.

The utility model also provides a microfluidic chip, comprising: a chip body and the microfluidic channel structure described in the above technical solution;

The micro-channel structure is arranged in the chip body;

The first liquid phase inlet, the gas phase inlet, the inlet of the second liquid phase flow channel, the inlet of the third liquid phase flow channel and the outlet of the fourth liquid phase flow channel are all is opened on the upper surface of the chip body.

Preferably, it also includes: a conveying device and an extracting device;

The delivery device includes a first delivery pump communicated with the first liquid phase inlet, a second delivery pump communicated with the gas phase inlet, and a second delivery pump communicated with the inlet of the second liquid phase flow channel. three delivery pumps and a fourth delivery pump communicated with the inlet of the third liquid phase flow channel;

The extraction device communicates with the outlet of the fourth liquid phase flow channel.

Preferably, the chip body includes a substrate and a cover;

The upper surface of the substrate is provided with the micro-channel structure;

The cover plate covers the upper surface of the substrate, and the first liquid phase inlet, the gas phase inlet, the inlet of the second liquid phase flow channel, and the third liquid phase flow channel The inlet and the outlet of the fourth liquid phase flow channel are opened on the cover plate.

In summary, the present invention provides a micro-channel structure, including: a micro-sphere focusing unit, an active valve quantitative control unit and a coaxial flow heterogeneous reaction unit; the micro-sphere focusing unit includes a first liquid phase The injection port and the vortex focusing curve are in communication with the first liquid phase injection port and the first end of the vortex focusing curve; the active valve quantitative control unit includes a first liquid phase flow channel, a gas phase inlet A sample port and a gas-phase flow channel, the first liquid-phase flow channel is communicated with the second end of the vortex focusing bend, the inner wall of the first liquid-phase flow channel is provided with a valve block, and the gas-phase injection port communicated with the first end of the gas-phase flow channel, the gas-phase flow channel is not communicated with the first liquid-phase flow channel, and the gas-phase flow channel is used to adjust the first liquid-phase flow channel provided with the valve block The opening and closing of the phase flow channel; the coaxial flow heterogeneous reaction unit includes a first liquid phase flow channel, a second liquid phase flow channel, a third liquid phase flow channel and a fourth liquid phase flow channel, and the first liquid phase flow channel Outlets of the second liquid phase flow channel and the third liquid phase flow channel communicate with the fourth liquid phase flow channel and are located outside the first liquid phase flow channel. The first liquid phase flow channel and the third liquid phase flow channel The four liquid phase flow channels are coaxial and the outlet of the first liquid phase flow channel is arranged at the center of the fourth liquid phase flow channel.

The microfluidic channel structure of the utility model comprises a microsphere focusing unit, an active valve quantitative control unit and a coaxial flow heterogeneous reaction unit. The microsphere focusing unit includes a vortex focusing curve, and the microsphere focusing unit focuses the microspheres to form a linear microsphere. The teams are arranged at equal intervals. The active valve quantitative control unit includes a first liquid-phase flow channel, a gas-phase injection port and a gas-phase flow channel. The inner wall of the first liquid-phase flow channel is provided with a valve block, and the gas-phase flow channel is used for Adjust the opening and closing of the first liquid phase flow channel provided with the valve block, the active valve quantitative control unit quantitatively disperses the microspheres focused into equal spacing, and the outlets of the second liquid phase flow channel and the third liquid phase flow channel The fourth liquid-phase flow channel is connected to and is located outside the first liquid-phase flow channel, the first liquid-phase flow channel and the fourth liquid-phase flow channel are coaxial, and the outlet of the first liquid-phase flow channel is arranged in the fourth liquid-phase flow channel At the center of the microspheres, the quantitatively dispersed microspheres are fully wrapped by the reaction solution in the second liquid phase flow channel and the third liquid phase flow channel to realize the quantitative heterogeneous reaction of the microspheres, and the microfluidic channel structure is airtight, The gas-phase flow channel and the first liquid-phase flow channel are not connected, and will not have any influence on the physicochemical properties of the contents of the droplets. The micro-flow channel structure of the utility model can break the limitation of insufficient and uneven reaction caused by the inability to precisely control the number of microspheres in the solid-liquid heterogeneous reaction of the traditional method, and can quickly generate a large number of precisely controlled micro-droplets, thereby realizing quantitative non-uniformity. Homogeneous reaction. Under the conditions of normal temperature and pressure, it can be quickly and accurately controlled by adjusting the microspheres of the first liquid phase flow channel in the quantitative control unit of the active valve, and the structure of the microchannel structure is simple, easy to manufacture in large quantities, and can be widely used in biomedicine. and light industry and chemical industry and many other fields.

Description of drawings

In order to illustrate the embodiments of the present invention or the technical solutions in the prior art more clearly, the following briefly introduces the accompanying drawings that are required to be used in the description of the embodiments or the prior art.

1 is a schematic diagram of a micro-channel structure provided in an embodiment of the present invention;

2 is a schematic diagram of a vortex focusing curve in a microfluidic channel structure provided in an embodiment of the present invention;

3 is a schematic diagram of the working principle of an active valve quantitative control unit in a micro-channel structure provided in an embodiment of the present invention;

4 is a schematic diagram of the overall structure of a microfluidic chip provided in an embodiment of the present invention;

Illustration description: 100. Microsphere focusing unit; 101. First liquid phase inlet; 102. Vortex focusing curve; 200. Active valve quantitative control unit; 201. First liquid phase flow channel; 202. Valve block 300. Coaxial flow heterogeneous reaction unit; 301. The outlet of the first liquid phase flow channel; 302. The fourth liquid phase flow channel 303. The second liquid-phase flow channel; 304. The third liquid-phase flow channel; 305. The inlet of the third liquid-phase flow channel; 306. The inlet of the second liquid-phase flow channel; outlet; 41. first delivery pump; 42. second delivery pump; 43. third delivery pump; 44. fourth delivery pump; 45. extraction device; 46. cover plate; 47. base plate.

Detailed ways

The utility model provides a microfluidic channel structure, a microfluidic control chip and a quantitative heterogeneous reaction method, which are used to solve the problem that the quantity of microspheres cannot be accurately and quantitatively controlled when the heterogeneous reaction is performed in the prior art.

The technical solutions in the embodiments of the present invention will be clearly and completely described below. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative work fall within the protection scope of the present invention.

Please refer to FIG. 1 and FIG. 2 , FIG. 1 is a schematic diagram of a micro-channel structure provided in an embodiment of the present invention, and FIG. 2 is a vortex focusing in a micro-channel structure provided in an embodiment of the present invention Schematic diagram of the bend.

An example of a microfluidic structure provided by the embodiment of the present invention includes: a microsphere focusing unit 100, an active valve quantitative control unit 200, and a coaxial flow heterogeneous reaction unit 300;

The microsphere focusing unit 100 includes a first liquid phase injection port 101 and a vortex focusing curve 102, and the first liquid phase injection port 101 is communicated with the first end of the vortex focusing curve 102;

The active valve quantitative control unit 200 includes a first liquid-phase flow channel 201, a gas-phase injection port 203 and a gas-phase flow channel 204, the first liquid-phase flow channel 201 communicates with the second end of the vortex focusing curve 102, and the first liquid-phase flow channel 201 The inner wall of the flow channel 201 is provided with a valve block 202, the gas-phase injection port 203 is communicated with the first end of the gas-phase flow channel 204, the gas-phase flow channel 204 is not communicated with the first liquid-phase flow channel 201, and the gas-phase flow channel 204 is used for adjustment The opening and closing of the first liquid phase flow channel 201 provided with the valve block 202;

The coaxial flow heterogeneous reaction unit 300 includes a first liquid phase flow channel 201 , a second liquid phase flow channel 303 , a third liquid phase flow channel 304 and a fourth liquid phase flow channel 302 . The outlet and the outlet of the third liquid-phase flow channel 304 are connected to the fourth liquid-phase flow channel 302 and are located outside the first liquid-phase flow channel 201. The first liquid-phase flow channel 201 and the fourth liquid-phase flow channel 302 are coaxial and The outlet 301 of the first liquid-phase flow channel is disposed at the center of the fourth liquid-phase flow channel 302 .

The microfluidic channel structure of the present invention includes a microsphere focusing unit 100, an active valve quantitative control unit 200 and a coaxial flow heterogeneous reaction unit 300. The microsphere focusing unit 100 includes a vortex focusing curve 102, and the microsphere focusing unit 100 will The microspheres are focused to form a linear array of microspheres and are arranged at equal intervals. The active valve quantitative control unit 200 includes a first liquid phase flow channel 201, a gas phase injection port 203 and a gas phase flow channel 204. The first liquid phase flow channel 201 has a The inner wall is provided with a valve block 202, the gas phase flow channel 204 is used to adjust the opening and closing of the first liquid phase flow channel 201 provided with the valve block 202, and the active valve quantitative control unit 200 quantitatively disperses the microspheres focused into equidistant arrangements. , the outlets of the second liquid-phase flow channel 303 and the third liquid-phase flow channel 304 are connected to the fourth liquid-phase flow channel 302 and are located outside the first liquid-phase flow channel 201. The first liquid-phase flow channel 201 and the fourth liquid-phase flow channel The flow channel 302 is coaxial and the outlet 301 of the first liquid phase flow channel is arranged at the center of the fourth liquid phase flow channel 302, and then passes through the reaction liquid in the second liquid phase flow channel 303 and the third liquid phase flow channel 304. The quantitatively dispersed microspheres are fully wrapped to realize the quantitative heterogeneous reaction of the microspheres, and the microfluidic channel structure is airtight, and the gas-phase channel 204 and the first liquid-phase channel 201 are not connected, which has a negative impact on the content of the microdroplets. Physicochemical properties will not have any effect. The micro-flow channel structure of the utility model can break the limitation of insufficient and uneven reaction caused by the inability to precisely control the number of microspheres in the solid-liquid heterogeneous reaction of the traditional method, and can quickly generate a large number of precisely controlled micro-droplets, thereby realizing quantitative non-uniformity. Homogeneous reaction. Under normal temperature and normal pressure conditions, the microspheres in the first liquid phase flow channel 201 in the active valve quantitative control unit 200 can be adjusted quickly and accurately, and the structure of the micro flow channel structure is simple, easy to produce in large quantities, and can be widely used in Biomedicine and light chemical industry and many other fields.

In the embodiment of the present invention, the first liquid phase inlet 101 can be externally connected to the microsphere suspension. In order to keep the concentration of the microsphere suspension relatively uniform, methods such as solvent dispersion or continuous magnetic stirring and ultrasonic dispersion can be used in the sample preparation process. The microsphere suspension enters the vortex focusing bend 102 through the first liquid phase injection port 101 to stabilize the dispersion of the microsphere suspension.

In the embodiment of the present invention, the valve blocks 202 and the gas-phase flow channels 204 have the same number;

The number of valve blocks 202 is two or more, preferably two.

In the embodiment of the present invention, the active valve quantitative control unit 200 further includes a gas buffer chamber 205;

The gas buffer chamber 205 is communicated with the second end of the gas phase flow channel 204 , and the gas buffer chamber 205 can adjust the opening and closing of the first liquid phase flow channel 201 provided with the valve block 202 through the gas buffer chamber 205 .

In the embodiment of the present invention, the material of the gas buffer chamber 205 and the first liquid phase flow channel 201 is an elastic material, preferably polydimethylsiloxane (PDMS), rubber or polyethylene (PE).

The setting of the gas buffer chamber 205 in the embodiment of the present invention is to prevent the wall surface of the first liquid phase flow channel 201 from being damaged due to the direct contact of the gas phase flow channel 204 with the wall surface of the first liquid phase flow channel 201 . When the gas buffer chamber 205 is not working, the gas buffer chamber 205 and the wall of the first liquid phase flow channel 201 keep a distance, and the distance between the gas buffer chamber 205 and the wall of the first liquid phase flow channel 201 is 30 μm˜100 μm.

Please refer to FIG. 3 , which is a schematic diagram of the working principle of the active valve quantitative control unit 200 in a micro-channel structure provided in an embodiment of the present invention. The gas phase injection port 203 is connected to an external pneumatic pump and uses inert gas. One end of the gas phase flow channel 204 is directly connected to the gas phase injection port 203 , and the other end of the gas phase flow channel 204 is connected to the gas buffer chamber 205 . The flow rate of the air flow is adjusted by a pneumatic pump. When the inert gas fills the entire gas phase flow channel 204, the continuous filling of the gas will cause the pressure of the gas buffer chamber 205 to increase, thereby forming pressure on the wall surface of the first liquid phase flow channel 201, forcing the first liquid phase flow channel 201. The wall surface of the phase flow channel 201 is bent in contact with the valve block 202 to block the liquid flow; after a period of time, the pneumatic pump stops outputting gas and performs corresponding degassing treatment, then the wall surface of the first liquid phase flow channel 201 recovers, and the microspheres are suspended The liquid can continue to pass through, thereby realizing quantitative control.

The inlets of the second liquid-phase flow channel 303 and the third liquid-phase flow channel 304 are externally connected with the reaction liquid, and are fully mixed with the microsphere suspension that has been quantitatively controlled, so as to realize a quantitative heterogeneous reaction with precise control.

In the embodiment of the present invention, the quantitatively controlled microsphere suspension enters the fourth liquid phase flow channel 302 with a larger diameter, the second liquid phase flow channel 303 and the third liquid phase flow channel through the first liquid phase flow channel 201 The outlet of the channel 304 communicates with the fourth liquid-phase flow channel 302 and is located outside the first liquid-phase flow channel 201 . Preferably, the included angle between the second liquid phase flow channel 303 and the third liquid phase flow channel 304 and the first liquid phase flow channel 201 is 45°.

In the embodiment of the present invention, the valve block 202 is a rectangular parallelepiped valve block;

The contact surface of the gas buffer chamber 205 with the first liquid phase flow channel 201 is a curved surface in the working state. The curved surface can increase the contact area between the gas buffer chamber 205 and the first liquid phase flow channel 201, reduce the pressure, can effectively prevent the flow channel wall surface of the first liquid phase flow channel 201 from breaking due to concentrated force, and can achieve The cuboid valve block is fully contacted to ensure precise control of the number of microspheres.

In the embodiment of the present invention, the cross-sectional shapes of the vortex focusing curve 102, the first liquid phase flow channel 201 and the gas phase flow channel 204 are the same;

The heights of the vortex focusing curve 102 , the first liquid-phase flow channel 201 and the gas-phase flow channel 204 are the same and are 100 μm˜200 μm.

Further, the cross-sectional shapes of the vortex focusing curve 102 , the first liquid phase flow channel 201 , the gas phase flow channel 204 , the second liquid phase flow channel 303 , the third liquid phase flow channel 304 and the fourth liquid phase flow channel 302 Likewise, the cross-sectional shape is preferably rectangular;

The heights of the vortex focusing curve 102 , the first liquid phase flow channel 201 , the gas phase flow channel 204 , the second liquid phase flow channel 303 , the third liquid phase flow channel 304 and the fourth liquid phase flow channel 302 are the same and are 100 μm ~200 μm.

In the embodiment of the present invention, the total length of the vortex focusing curve 102 is 100mm˜1000mm;

The width of the vortex focusing curve 102 is 100 μm˜200 μm;

The distance between two adjacent flow channels of the vortex focusing curve 102 is 100 μm˜300 μm;

The radius of curvature of the innermost flow channel of the vortex focusing curve 102 is 20 mm to 30 mm.

In the embodiment of the present invention, the second liquid phase flow channel 303 and the third liquid phase flow channel 304 and the first liquid phase flow channel 201 form a coaxial flow in the fourth liquid phase flow channel 302, and the first liquid phase flow channel 201 The width of the second liquid phase flow channel 303 and the third liquid phase flow channel 304 are both 50 μm to 150 μm, and the width of the fourth liquid phase flow channel 302 is 200 μm to 300 μm.

The width of the gas-phase flow channel 204 is 50 μm to 100 μm.

The ratio of the particle size of the microspheres in the microsphere suspension to the height of the first liquid phase flow channel 201 is not greater than 0.4.

The above is a detailed description of an embodiment of a microfluidic channel structure provided by an embodiment of the present invention, and an embodiment of a microfluidic chip provided by an embodiment of the present invention will be described in detail below.

An embodiment of a microfluidic chip provided by the embodiment of the present invention includes: a chip body and a microfluidic channel structure of the above technical solution;

The micro-channel structure is arranged in the chip body;

The first liquid phase injection port 101, the gas phase injection port 203, the inlet 306 of the second liquid phase flow channel, the inlet 305 of the third liquid phase flow channel and the outlet 307 of the fourth liquid phase flow channel are all opened on the chip body. upper surface.

In the embodiment of the present utility model, it also includes: a conveying device and an extracting device 45;

The delivery device includes a first delivery pump 41 communicated with the first liquid phase inlet 101, a second delivery pump 42 communicated with the gas phase inlet 203, and a third delivery pump communicated with the inlet 306 of the second liquid phase flow channel 43 and a fourth delivery pump 44 communicating with the inlet 305 of the third liquid phase flow channel;

The extraction device 45 communicates with the outlet 307 of the fourth liquid phase flow channel.

Through the delivery device and the extraction device 45, the sample input can be performed at a constant speed and the fluid in the microfluidic chip can be stably extracted, so as to ensure a stable focusing effect, so that the inlets of each phase and all the outlet pressures of the microfluidic chip remain coherent and Consistently, keep the pressure and flow rate of the fluid in the chip device constant.

In the embodiment of the present invention, the chip body includes a base plate 47 and a cover plate 46;

The upper surface of the substrate 47 is provided with a micro-channel structure;

The cover plate 46 covers the upper surface of the substrate 47, and the first liquid phase injection port 101, the gas phase injection port 203, the inlet 306 of the second liquid phase flow channel, the inlet 305 of the third liquid phase flow channel and the fourth liquid phase flow channel. The outlet 307 of the phase flow channel is opened on the cover plate 46 .

The above is a detailed description of an embodiment of a microfluidic chip provided by the embodiment of the present invention, and an embodiment of a quantitative heterogeneous reaction method provided by the embodiment of the present invention will be described in detail below. .

An application example of a microfluidic chip provided by the embodiment of the present invention adopts the microfluidic channel structure of the above technical solution, and includes the following steps:

S1: Disperse the microsphere suspension through the vortex focusing curve 102 and flow into the first liquid phase flow channel 201 of the active valve quantitative control unit 200;

S2: adjusting the opening and closing of the first liquid phase flow channel 201 provided with the valve block 202 through the gas phase flow channel 204 to control the number of microspheres in the microsphere suspension flowing into the coaxial flow heterogeneous reaction unit 300;

S3: The reaction solution enters the fourth liquid phase flow channel 302 coaxial with the first liquid phase flow channel 201 through the second liquid phase flow channel 303 and the third liquid phase flow channel 304, and exits from the first liquid phase flow channel 201 The quantitative microspheres undergo a heterogeneous reaction to obtain microdroplets.

In the application example of the present invention, the microsphere suspension is dispersed through the vortex focusing bend 102 through the first liquid phase inlet 101 , and the microsphere suspension is dispersed through the inlets of the second liquid phase flow channel 303 and the third liquid phase flow channel 304 to the first liquid phase flow channel 304 . The second liquid phase flow channel 303 and the third liquid phase flow channel 304 pass into the reaction liquid, and a pneumatic pump is used to pass gas into the gas phase flow channel 204 through the gas phase inlet 203 , and the quantitative droplets flow out from the outlet of the extraction device 45 .

A gas buffer chamber 205 separates the gas-phase flow channel 204 and the first liquid-phase flow channel 201. The gas-phase flow channel 204 is subjected to the action of the gas pressure input by the pneumatic pump, and the gas buffer chamber 205 has pressure to the first liquid-phase flow channel 201. Generated, the wall surface of the first liquid phase flow channel 201 is forced to be bent and contacted with the valve block 202 on the wall surface, and the liquid flow is blocked to achieve the effect of dispersing and controlling the microspheres. , the number of microspheres can be precisely and quantitatively controlled.

The microspheres are subjected to the combined action of Dean's drag force and inertial lift force in the vortex focusing curve 102, and a linear microsphere array is formed in the vortex focusing curve 102 and arranged at equal intervals at a fixed position. After being controlled by the active valve quantitative control unit 200, the reaction liquids in the second liquid-phase flow channel 303 and the third liquid-phase flow channel 304 flow into the fourth liquid-phase flow channel 302 with a larger diameter, and the second liquid-phase flow channel 303 The reaction liquid with the third liquid phase flow channel 304 first enters the fourth liquid phase flow channel 302, and then mixes with the microspheres precisely controlled by the active valve quantitative control unit 200, and the quantitative microspheres are filled with the fourth liquid phase flow at the confluence point. The reaction solution in channel 302 is fully wrapped, and the heterogeneous reaction is carried out to obtain microdroplets. The prepared droplets can be collected by the extraction device 45 through the outlet 307 of the fourth liquid phase flow channel.

The microfluidic chip of the utility model is highly integrated, the entire chip area is small, only a few cubic centimeters; the microfluidic chip has low cost, simple structure and easy mass production. Using the microfluidic chip of the utility model to carry out heterogeneous reaction, the consumption of reagents is small, only at the microliter level. The quantitative heterogeneous reaction method of the present invention is precisely controlled, the height and spacing of the microsphere array passing through the vortex focusing curve 102 are uniform, and by adjusting the pneumatic pump, the number of microspheres in the microsphere suspension can be precisely controlled, Accurate and controllable quantitative control is realized; the method of quantitative heterogeneous reaction is environmentally friendly. During the operation, the focusing, dispersing and quantitative control of the microspheres are controlled by mechanical principles, and the functional activity and physical and chemical properties of the microspheres are not affected. The manufacturing materials of the microfluidic chip are harmless to the environment; the microfluidic chip can be made of transparent materials, which can be directly observed with a microscope, and can also be recorded with a high-speed camera, which is easy to operate and easy to observe. ;Because the gas and the microsphere suspension are spaced apart, the gas will not have any effect on the microsphere suspension and can be precisely controlled, which is suitable for a large number of heterogeneous reactions and has strong adaptability; vortex focusing curve 102 per second The clock can realize the output of hundreds of dispersedly arranged microspheres, and the process is continuous, the output per unit time is high, and it is continuous and fast.

In order to further understand the present utility model, the present utility model will be described in detail below with reference to specific embodiments.

Example 1

The material of the microfluidic chip in this embodiment is polydimethylsiloxane (PDMS), wherein the total length of the vortex focusing curve is 400 mm, and the distance between two adjacent flow channels of the vortex focusing curve is 150 μm. The radius of curvature of the innermost flow channel of the vortex focusing curve is 20mm, the width of the gas-phase flow channel, the second liquid-phase flow channel and the third liquid-phase flow channel are all 50 μm, and the width of the first liquid-phase flow channel is 100 μm. The width of the fourth liquid phase flow channel is 200 μm, the distance between the gas buffer chamber and the wall of the first liquid phase flow channel is 40 μm in the non-working state, and the height of all the flow channels is 100 μm.

The microfluidic chip of this embodiment is used to perform quantitative heterogeneous reaction, which specifically includes: selecting nitrogen gas as the gas phase, the solid phase of the microsphere suspension being titania microspheres with a particle size of 30 μm, the methyl blue aqueous solution as the reaction solution, and using an external light source at the same time Perform continuous light treatment. The microsphere suspension and the reaction solution were injected into the chip using a Teflon capillary tube, and the gas phase fluid was controlled by a pneumatic pump. The flow rate of the microsphere suspension is 30 μl/min, the gas phase flow rate is 50 μl/min, and the flow rate of the reaction liquid in the second liquid phase flow channel and the third liquid phase flow channel are both 30 μl/min. The quantitative titanium dioxide microspheres in the microsphere suspension and the methyl blue are accurately, efficiently and fully reacted heterogeneously under light conditions to obtain methylene blue or methylene blue.

Example 2

The material of the microfluidic chip in this embodiment is polydimethylsiloxane (PDMS), wherein the total length of the vortex focusing curve is 600 mm, and the distance between two adjacent flow channels of the vortex focusing curve is 200 μm. The radius of curvature of the innermost flow channel of the rotary focusing curve is 20mm, the width of the gas phase flow channel is 50μm, the width of the second liquid phase flow channel and the third liquid phase flow channel are both 20μm, and the width of the first liquid phase flow channel is 20μm. is 50 μm, the width of the fourth liquid phase flow channel is 90 μm, the distance between the gas buffer chamber and the wall of the first liquid phase flow channel is 40 μm in the non-working state, and the height of all the flow channels is 80 μm.

The microfluidic chip of this embodiment is used to perform quantitative heterogeneous reaction, which specifically includes: selecting nitrogen gas as gas phase, and calcium hydroxide [Ca(OH) ₂ ] solution containing carbon spheres with a particle size of 20 μm, that is, carbon sphere suspension as microspheres Suspension (carbon balls do not react with calcium hydroxide) to form a dispersed phase, and ammonium carbonate solution is used as a reaction solution to form a continuous liquid phase. The carbon sphere suspension and the ammonium carbonate solution were injected into the chip using a Teflon capillary tube, respectively, and the gas-phase fluid was controlled by a pneumatic pump. The flow rate of the microsphere suspension injected into the first liquid phase flow channel is 40 μL/min, the gas phase injection flow rate is 50 μL/min, and the flow rate of the ammonium carbonate solution injected into the second liquid phase flow channel and the third liquid phase flow channel is 80 μL/min , by adjusting the flow rate of the gas phase and the microsphere suspension, various microsphere suspensions with quantitative carbon sphere content can be obtained, and the coaxial flow is fully mixed with the continuous phase of ammonium carbonate for a heterogeneous, accurate and efficient reaction, and calcium carbonate is precipitated on the surface of the carbon spheres [ CaCO ₃ ] precipitation evenly wrapped the carbon spheres, and at the same time the heterogeneous reaction was uniform, and microdroplets with quantitative carbon sphere content could be obtained.

The above are only the preferred embodiments of the present invention. It should be pointed out that for those skilled in the art, without departing from the principles of the present invention, several improvements and modifications can be made. These improvements and Retouching should also be regarded as the protection scope of the present invention.

Claims (9)

1. A micro flow channel structure for quantitative heterogeneous reactions, comprising: the device comprises a microsphere focusing unit, an active valve quantitative control unit and a coaxial flow heterogeneous reaction unit;

the microsphere focusing unit comprises a first liquid phase sample inlet and a vortex focusing curve, and the first liquid phase sample inlet is communicated with a first end of the vortex focusing curve;

the quantitative control unit of the active valve comprises a first liquid phase flow channel, a gas phase sample inlet and a gas phase flow channel, the first liquid phase flow channel is communicated with the second end of the vortex focusing bend, a valve block is arranged on the inner wall of the first liquid phase flow channel, the gas phase sample inlet is communicated with the first end of the gas phase flow channel, the gas phase flow channel is not communicated with the first liquid phase flow channel, and the gas phase flow channel is used for adjusting the opening and closing of the first liquid phase flow channel provided with the valve block;

the coaxial flow heterogeneous reaction unit comprises a first liquid phase flow channel, a second liquid phase flow channel, a third liquid phase flow channel and a fourth liquid phase flow channel, wherein an outlet of the second liquid phase flow channel and an outlet of the third liquid phase flow channel are communicated with the fourth liquid phase flow channel and are positioned on the outer side of the first liquid phase flow channel, the first liquid phase flow channel and the fourth liquid phase flow channel are coaxial, and an outlet of the first liquid phase flow channel is arranged at the center of the fourth liquid phase flow channel.

2. The micro flow channel structure for quantitative heterogeneous reaction according to claim 1, wherein the number of the valve blocks and the number of the gas phase flow channels are the same;

the number of the valve blocks is more than two.

3. The micro flow channel structure for quantitative heterogeneous reactions according to claim 1, wherein the active valve quantitative control unit further comprises a gas buffer chamber;

the gas buffer chamber is communicated with the second end of the gas phase flow channel, and the gas phase flow channel can be adjusted to be provided with the valve block to open and close at the first liquid phase flow channel through the gas buffer chamber.

4. The micro flow channel structure for quantitative heterogeneous reactions according to claim 3, wherein the valve block is a rectangular parallelepiped valve block;

and the contact surface of the gas buffer chamber and the first liquid phase flow channel is a curved surface in a working state.

5. The micro flow channel structure for quantitative heterogeneous reactions according to claim 1, wherein the cross-sectional shapes of the vortex focusing curve, the first liquid phase flow channel and the gas phase flow channel are the same;

the heights of the vortex focusing curved channel, the first liquid phase flow channel and the gas phase flow channel are the same and are 100-200 mu m.

6. The micro flow channel structure for quantitative heterogeneous reactions according to claim 1, wherein the total length of the vortex focusing curve is 100mm to 1000 mm;

the width of the vortex focusing curve is 100-200 μm;

the distance between two adjacent runners of the vortex focusing curved channel is 100-300 mu m;

the curvature radius of the innermost runner of the vortex focusing curved channel is 20-30 mm.

7. A microfluidic chip, comprising: a chip body and the micro flow channel structure of any one of claims 1 to 6;

the micro-channel structure is arranged in the chip body;

the first liquid phase sample inlet, the gas phase sample inlet, the inlet of the second liquid phase flow channel, the inlet of the third liquid phase flow channel and the outlet of the fourth liquid phase flow channel are all arranged on the upper surface of the chip body.

8. The microfluidic chip according to claim 7, further comprising: a conveying device and an extracting device;

the conveying device comprises a first conveying pump communicated with the first liquid phase sample injection port, a second conveying pump communicated with the gas phase sample injection port, a third conveying pump communicated with an inlet of the second liquid phase flow channel and a fourth conveying pump communicated with an inlet of the third liquid phase flow channel;

the extraction device is communicated with an outlet of the fourth liquid phase flow channel.

9. The microfluidic chip according to claim 7, wherein the chip body comprises a substrate and a cover plate;

the micro-channel structure is arranged on the upper surface of the substrate;

the apron covers the upper surface of base plate, just first liquid phase introduction port gas phase introduction port the import of second liquid phase runner the import of third liquid phase runner with the export of fourth liquid phase runner is seted up in on the apron.

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