CN215593090U - Cell culture device - Google Patents
Cell culture device Download PDFInfo
- Publication number
- CN215593090U CN215593090U CN202122238923.9U CN202122238923U CN215593090U CN 215593090 U CN215593090 U CN 215593090U CN 202122238923 U CN202122238923 U CN 202122238923U CN 215593090 U CN215593090 U CN 215593090U
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- CN
- China
- Prior art keywords
- culture dish
- culture
- magnetic sheet
- top cover
- dish body
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- 238000004113 cell culture Methods 0.000 title claims abstract description 22
- 238000001914 filtration Methods 0.000 claims abstract description 8
- 239000012528 membrane Substances 0.000 claims abstract description 8
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 7
- 239000000741 silica gel Substances 0.000 claims description 7
- 229910002027 silica gel Inorganic materials 0.000 claims description 7
- 229920001296 polysiloxane Polymers 0.000 claims 1
- 238000009423 ventilation Methods 0.000 abstract description 6
- 241000894006 Bacteria Species 0.000 abstract description 3
- 241000700605 Viruses Species 0.000 abstract description 3
- 239000000428 dust Substances 0.000 abstract description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 3
- 238000000034 method Methods 0.000 description 5
- 239000007788 liquid Substances 0.000 description 4
- 238000011109 contamination Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000002474 experimental method Methods 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- BELBBZDIHDAJOR-UHFFFAOYSA-N Phenolsulfonephthalein Chemical compound C1=CC(O)=CC=C1C1(C=2C=CC(O)=CC=2)C2=CC=CC=C2S(=O)(=O)O1 BELBBZDIHDAJOR-UHFFFAOYSA-N 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 238000007664 blowing Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000010367 cloning Methods 0.000 description 1
- 238000012411 cloning technique Methods 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000006185 dispersion Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
Images
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a cell culture device, which relates to the technical field of cell culture and comprises a culture dish body, wherein a top cover is arranged at the top end of the culture dish body, a connecting piece is fixedly connected between one end of the top cover and one end of the outer part of a culture body, a ventilation opening is formed in the top of the top cover, an air filtering membrane is fixedly connected in the ventilation opening, a first magnetic sheet is embedded on the outer side of the culture dish body, and a second magnetic sheet corresponding to the first magnetic sheet is embedded on the inner side wall of the top cover. According to the utility model, the culture dish body and the top cover are respectively provided with the first magnetic sheet and the second magnetic sheet, so that the culture dish body and the top cover can be tightly connected, and the air-permeable port and the air filtering membrane are arranged, so that dust, water vapor, bacteria and viruses in the air can be isolated, a good culture environment can be maintained, and cells in the culture dish can be effectively prevented from being infected.
Description
Technical Field
The utility model relates to the technical field of cell culture, in particular to a cell culture device.
Background
Cell culture (cellcell) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions, etc.) in vitro to make it survive, grow, reproduce and maintain the main structure and function. Cell culture is an essential process for both the whole bioengineering technique and one of the biological cloning techniques, and is itself a large-scale cloning of cells. Taking certain tissue cells from a body under a sterile environment (according to the purpose of an experiment), inoculating the tissue cells into a culture vessel after certain treatment (such as digestion, cell dispersion, separation and the like), inoculating tissue blocks into the bottom of the culture vessel, sticking the tissue blocks onto the bottom after several hours, adding a culture medium, and immediately putting the cells into an incubator after the cells enter the culture vessel so as to enable the cells to enter a growth state as early as possible. The cells being cultured should be observed at regular intervals, including whether the cells are growing well, whether the morphology is normal, whether there is contamination, whether the pH of the medium is too acidic or too basic (indicated by phenol red indicator), and also the culture temperature and CO2 concentration should be checked at regular intervals. The usage rate of the culture dish in the cell culture experiment is higher than that of the culture bottle, and the culture dish is more convenient for the blowing operation of the cells and the cell collection is more thorough. The culture dish consists of a flat disc-shaped bottom and a lid.
When the cells are cultured, the cells absorb and generate gas so as to change the gas and liquid environment in the culture dish. In the prior art, a gap exists between a culture body and a culture dish cover, so that gas exchange during cell culture is facilitated, but the gap is large, and fungi in the air easily enter a culture dish to cause cell pollution; liquid is easy to leak from the gap by shaking in the moving process of the culture dish, and pollution is also caused. In addition, the culture body and the culture dish cover of the culture dish are separated, so that the culture dish cover is easy to open to cause cell pollution when the culture dish is moved; when a plurality of culture dishes are operated, the culture dish cover is easy to cover by mistake, and cell pollution can be caused. There is therefore a need for further improvements in cell culture dishes to address the problems described above.
SUMMERY OF THE UTILITY MODEL
The present invention provides a cell culture apparatus that solves the problems set forth in the background art described above.
In order to achieve the purpose, the utility model provides the following technical scheme:
the utility model provides a cell culture device, includes the culture dish body, the top of culture dish body is equipped with the top cap, fixedly connected with connecting piece between the one end of top cap and the outside one end of culture body, and ventilative mouthful has been seted up at the top of top cap, fixedly connected with filtration membrane in the ventilative mouthful.
As a preferable technical scheme of the utility model, a first magnetic sheet is embedded on the outer side of the culture dish body, and a second magnetic sheet corresponding to the first magnetic sheet is embedded on the inner side wall of the top cover.
As a preferable technical scheme of the utility model, the handle is fixedly connected to the outside of the top cover.
As a preferred technical scheme of the utility model, the top end of the culture dish body is fixedly connected with a silica gel pad.
As a preferred technical scheme of the utility model, the connecting piece is made of silica gel.
The utility model has the following advantages: according to the utility model, the culture dish body and the top cover are respectively provided with the first magnetic sheet and the second magnetic sheet, so that the culture dish body and the top cover can be tightly connected, and the air-permeable port and the air filtering membrane are arranged, so that dust, water vapor, bacteria and viruses in the air can be isolated, a good culture environment can be maintained, and cells in the culture dish can be effectively prevented from being infected.
Drawings
FIG. 1 is a schematic view of the structure of a cell culture apparatus.
FIG. 2 is a cross-sectional view of a top cover in the cell culture apparatus.
In the figure: 1. a culture dish body; 2. a top cover; 3. a connecting member; 4. a ventilation opening; 5. a handle; 6. a first magnetic sheet; 7. a second magnetic sheet; 8. an air filtration membrane; 9. a silica gel pad.
Detailed Description
The preferred embodiments of the present invention will be described in conjunction with the accompanying drawings, and it will be understood that they are described herein for the purpose of illustration and explanation and not limitation.
It should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience in describing the present invention and simplifying the description, but do not indicate or imply that the referred devices or elements must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention.
Referring to fig. 1-2, a cell culture device comprises a culture dish body 1, a top cover 2 is arranged at the top end of the culture dish body 1, a connecting piece 3 is fixedly connected between one end of the top cover 2 and one end of the outside of a culture body, a ventilation opening 4 is formed in the top of the top cover 2, and an air filtering membrane 8 is fixedly connected in the ventilation opening 4.
The outside of culture dish body 1 inlays and is equipped with first magnetic sheet 6, inlays on the inside wall of top cap 2 and is equipped with the second magnetic sheet 7 corresponding with first magnetic sheet 6. The first magnetic sheet 6 and the second magnetic sheet 7 are provided with a plurality of magnetic sheets. Through the magnetic attraction effect of the first magnetic sheet 6 and the second magnetic sheet 7, the compactness of the connection between the top cover 2 and the culture dish body 1 can be enhanced.
The handle 5 is fixedly connected with the outer part of the top cover 2. Facilitating the opening of the top cover 2.
The top end of the culture dish body 1 is fixedly connected with a silica gel pad 9. After the cover body covers the culture dish body 1, the joint of the top cover 2 and the culture dish body 1 can be sealed by the silica gel gasket 9, the shaking of the top cover 2 can be reduced, the phenomenon that liquid is shaken to cause the leakage of cell culture liquid in the moving process of the culture dish body 1 can be avoided, and the risk of cell contamination is reduced.
The connecting piece 3 is made of silica gel. Can avoid the wrong lid of culture dish body 1 and top cap 2 separation back lid to lead to cell contamination through setting up connecting piece 3.
According to the utility model, the first magnetic sheet 6 and the second magnetic sheet 7 are respectively arranged on the culture dish body 1 and the top cover 2, so that the culture dish body 1 and the top cover 2 can be tightly connected, and the ventilation port 4 and the air filtering membrane 8 are arranged, so that dust, water vapor, bacteria and viruses in the air can be isolated, a good culture environment can be maintained, and cells in the culture dish can be effectively prevented from being infected.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that changes may be made in the embodiments and/or equivalents thereof without departing from the spirit and scope of the utility model. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. The utility model provides a cell culture device, includes the culture dish body, its characterized in that, the top of culture dish body is equipped with the top cap, fixedly connected with connecting piece between the one end of top cap and the outside one end of culture body, and ventilative mouthful, fixedly connected with filtration membrane in the ventilative mouthful have been seted up at the top of top cap.
2. The cell culture device of claim 1, wherein a first magnetic sheet is embedded on the outer side of the culture dish body, and a second magnetic sheet corresponding to the first magnetic sheet is embedded on the inner side wall of the top cover.
3. The cell culture apparatus of claim 1, wherein the cap is fixedly attached to the exterior of the cap by a handle.
4. The cell culture device of claim 1, wherein a silica gel pad is fixedly connected to the top end of the culture dish body.
5. The cell culture apparatus of claim 1, wherein the connector is made of silicone.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122238923.9U CN215593090U (en) | 2021-09-15 | 2021-09-15 | Cell culture device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202122238923.9U CN215593090U (en) | 2021-09-15 | 2021-09-15 | Cell culture device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN215593090U true CN215593090U (en) | 2022-01-21 |
Family
ID=79886038
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202122238923.9U Active CN215593090U (en) | 2021-09-15 | 2021-09-15 | Cell culture device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN215593090U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115261227A (en) * | 2022-08-15 | 2022-11-01 | 河北医科大学第一医院 | Portable cell culture case |
-
2021
- 2021-09-15 CN CN202122238923.9U patent/CN215593090U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115261227A (en) * | 2022-08-15 | 2022-11-01 | 河北医科大学第一医院 | Portable cell culture case |
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