CN215492690U

CN215492690U - Plant root secretion and plant volatile gas continuous collecting and culturing device

Info

Publication number: CN215492690U
Application number: CN202120156227.0U
Authority: CN
Inventors: 李奇松; 李家俊; 粟传兵
Original assignee: Wuyi University Fujian
Current assignee: Wuyi University Fujian
Priority date: 2021-01-21
Filing date: 2021-01-21
Publication date: 2022-01-11
Anticipated expiration: 2031-01-21

Abstract

The utility model relates to a device for continuously collecting and culturing plant root exudates and plant volatile gas, which comprises the following components: the device comprises a culture device, wherein the upper part of the culture device is connected with a gas exchange and collection device through a pipeline, a volatile gas adsorption column is connected onto the gas exchange and collection device, the middle part of the culture device is connected with a nutrition adding device through a pipeline, the bottom of the culture device is connected with a liquid collection device through a pipeline, vermiculite is stacked at the bottom of the culture device, the culture device comprises a culture dish, an isolation cover is clamped on the culture dish, the vermiculite is stacked on the culture dish, and a liquid interface is arranged on the side wall of the bottom of the culture dish.

Description

Plant root secretion and plant volatile gas continuous collecting and culturing device

Technical Field

The utility model relates to a device for continuously collecting and culturing plant root exudates and plant volatile gases.

Background

The effective collection of root exudates is the most basic step for developing the interaction research between the root exudates and the soil environment, and the currently reported extraction modes are more. Because some root exudate components are unstable, such as unstable self, and are decomposed by external microorganisms, the types and the quantities of the finally obtained root exudate components in different extraction modes can have larger difference. The main methods at present include methods of water culture extraction, in-situ extraction, fresh soil extraction, air-dried soil extraction and the like, and the collection of root exudates of the same plant is one-time collection for interrupting plant growth and is complicated in operation, wherein the water culture collection is separated from the primary growth environment to cause the plant to be stressed outside, the components of the root exudates are changed, and the collection is insufficient, so that the continuous collection is complicated and inaccurate. The extraction of plant volatile gas is the most basic step for developing the signal substance research of the dialogue among plants and the pest and disease stress, and the extraction mode reported at present is less. Because the gas molecules move fast after the gas is diffused, the gas molecules are not easy to collect and enrich, meanwhile, the real-time collection of the diffused gas of the plants is difficult, and the difficulty of continuous collection is higher.

Disclosure of Invention

The utility model provides a device for continuously collecting and culturing plant root exudates and plant volatile gas, which solves the difficulty of continuously collecting the plant root exudates and the plant volatile gas under the condition of not interfering the normal growth of plants; the problem that the plant is stressed outside due to leaving the primary growth environment, so that the interference on root exudates and plant volatile gas components is solved; the difficulty that the volatile gas is difficult to collect and enrich due to too fast volatilization in the process of collecting the plant volatile gas is solved, and the difficulty that the volatile gas of the plant is continuously collected under the condition of not interfering the normal growth of the plant is solved.

The utility model solves the technical problem by adopting the scheme that a device for continuously collecting and culturing plant root exudates and plant volatile gas comprises: the device comprises a culture device, wherein the upper part of the culture device is connected with a gas exchange and collection device through a pipeline, the middle part of the culture device is connected with a nutrition adding device through a pipeline, the bottom of the culture device is connected with a liquid collection device through a pipeline, and vermiculite is stacked at the bottom in the culture device.

Further, culture apparatus includes the culture dish, and the joint has the cage on the culture dish, and vermiculite is stacked on the culture dish, has seted up the liquid interface on the lateral wall of culture dish bottom.

Furthermore, a nutrient solution interface is arranged on the side wall of the bottom of the isolation cover, at least two gas exchange interfaces are arranged on the side part of the middle part of the isolation cover, and one of the gas exchange interfaces is connected with a gas exchange and collection device through a pipeline.

Furthermore, be provided with the nutrient solution interface on the lateral wall of cage bottom, be provided with two at least gas exchange interfaces on the lateral part of cage middle part, one of them gas exchange interface is connected with gas exchange and collection device through the pipeline, has connect volatility gas adsorption column in the pipeline.

Further, the gas exchange and collection device is a vacuum pump.

Furthermore, the connection part of the culture dish and the isolation cover is sealed by a sealing film.

Furthermore, the nutrition adding device comprises an infusion bottle, the infusion bottle is filled with nutrient solution, a butyl rubber plug is arranged on a nutrient solution interface, a medical infusion apparatus is connected below the infusion bottle, one end of the medical infusion apparatus is connected with the output end of the bottom of the infusion bottle, and the other end of the medical infusion apparatus penetrates through the nutrient solution interface butyl rubber plug and extends into the isolation hood to drop the nutrient solution into the device.

Furthermore, the liquid interface is plugged with a butyl rubber plug, the liquid collecting device comprises a sampling bottle, the sampling bottle is connected with the liquid interface through a liquid receiving pipe with a switch valve, one end of the liquid receiving pipe is connected with the input end of the sampling bottle, and the other end of the liquid receiving pipe penetrates through the butyl rubber plug of the liquid interface and extends into the culture dish.

Furthermore, the inward flange of the department of the upper end of culture dish is provided with the interior shoulder up, the outward flange of the lower extreme kneck of cage is provided with decurrent and interior shoulder complex outer convex shoulder, is provided with the joint spare of mutually supporting on the binding face of interior shoulder and outer convex shoulder, and the joint that realizes culture dish and cage through the cooperation of interior shoulder, outer convex shoulder is fixed.

Furthermore, the clamping piece comprises a clamping block and a clamping groove, the clamping groove is formed in the outer shoulder, and the clamping block is arranged on the inner shoulder.

Compared with the prior art, the utility model has the advantages that 1, the structure is simple, the design is reasonable, the installation, the disassembly and the cleaning are convenient, compared with common glass materials, the device has high cost performance, is not easy to damage and can be used for multiple times, and the environment-friendly characteristic is achieved; 3. culture apparatus, nutrition are added device and liquid collection device and are rationally linked up, can add the required nutrient substance of growth for the plant continuously, realize the continuous collection of root secretion simultaneously. 4. In the prior art, plant root exudates are collected after being harvested by adopting water culture and soil culture of plants, so that the plants leave the primary growth environment and are stressed by the outside, and the root exudates cannot be accurately and dynamically collected; 5. the utility model can continuously collect and dynamically monitor the volatile gas of the plant under the condition of not interfering the normal growth of the plant; 6. the device has good tightness among devices, can ensure that the plant is controllably cultured under the condition of not being polluted by external microorganisms, and can be applied to the research of the plant on root secretion and plant volatile gas under the stress of organisms (such as pathogenic bacteria) or abiotic organisms (such as different nutritional conditions).

Drawings

The utility model is further described with reference to the following figures.

FIG. 1 is a schematic view of the structure of the present invention;

FIG. 2 is a schematic view of the structure of the place where the culture dish and the isolation cover are matched.

In the figure: 1-a culture dish; 2-a shielding case; 3-vermiculite; 4-gas exchange and collection means; 5-a nutrient adding device; 6-a liquid collection device; 7-nutrient solution interface; 8-liquid interface; 9-butyl rubber plug; 10-gas exchange interface; 11-medical infusion apparatus; 12-a switching valve; 13-inner shoulder and neck; 14-external shoulder and clamping groove; 15-a volatile gas adsorption column; 16-hanging hooks; 17-sealing ring.

Detailed Description

The utility model is further described with reference to the following figures and detailed description.

As shown in fig. 1-2, a device for continuously collecting and culturing plant root exudates and plant volatile gases: the device comprises a culture device, wherein the upper part of the culture device is connected with a gas exchange and collection device 4 through a pipeline, the middle part of the culture device is connected with a nutrition adding device 5 through a pipeline, the bottom of the culture device is connected with a liquid collection device 6 through a pipeline, and vermiculite 3 is stacked at the bottom in the culture device; vermiculite is the planting substrate.

In the embodiment, the culture device comprises a culture dish 1, an isolation cover 2 is clamped on the culture dish, vermiculite is stacked on the culture dish, and a liquid interface 8 is arranged on the side wall of the bottom of the culture dish; the culture dish and the isolation cover are made of high-definition transparent PC materials and are cylindrical.

In this embodiment, be provided with nutrient solution interface 7 on the lateral wall of cage bottom, be provided with two at least gas exchange interfaces 10 on the lateral part of cage middle part, one of them gas exchange interface with be connected with gas exchange and collection device through the pipeline, when gas exchange and collection device are connected and are bled, the gas exchange interface that is connected with gas exchange and collection device is sucked away from in the device, then in fresh air gets into the device from another gas exchange interface, realize gaseous exchange, the material in the gas of taking out adsorbs on the adsorption column simultaneously, the collection and the enrichment of the volatile gas that just accomplish.

In this embodiment, the gas exchange interfaces are covered with filtration membranes, the filtration membranes are filtration membranes with a pore size of 0.22 μm, and the gas exchange and collection device is connected with a volatile gas adsorption column 15 (the filler is polydivinylbenzene porous beads GDX-101).

In this embodiment, the gas exchange and collection device is a vacuum pump, and the gas exchange and collection device is a guarantee for the healthy survival of plants, is a key device for collecting plant volatile gas, and is a water circulation vacuum pump for performing air exchange circulation through negative pressure.

In this embodiment, for reasonable design, the connection between the culture dish and the isolation cover is further sealed by a sealing film to ensure a sterile culture environment.

In this embodiment, the nutrition adding device comprises an infusion bottle, the infusion bottle is filled with nutrition liquid, a butyl rubber plug 9 is plugged on a nutrition liquid interface, a medical infusion apparatus 11 is connected below the infusion bottle, one end of the medical infusion apparatus is connected with the output end of the bottom of the infusion bottle, the other end of the medical infusion apparatus penetrates through the nutrition liquid interface butyl rubber plug and extends into the isolation hood, and nutrition liquid is dropped into the device; the infusion bottle is fixed on the top by a hook 16.

In this embodiment, the liquid interface facial make-up has the butyl plug, liquid collection device is the key device who draws root system secretion, including the sampling bottle, the sampling bottle is through the liquid receiving pipe that has ooff valve 12 and liquid interface connection, and liquid receiving pipe one end is connected with the input of sampling bottle, and the other end passes liquid interface butyl plug, stretches into in the culture dish, during the use, extracts the sampling bottle through aseptic syringe, can be more easily collect the root system secretion, the liquid that will collect carry out the secretion and draw can, greatly reduced the degree of difficulty of continuous collection.

In this embodiment, the inward flange of the last port department of culture dish is provided with upward interior shoulder 13, the outward flange of the lower extreme kneck of cage is provided with decurrent and interior shoulder complex outer shoulder 14, is provided with the joint spare of mutually supporting on the binding face of interior shoulder and outer shoulder, realizes through the cooperation of interior shoulder, outer shoulder that the joint of culture dish and cage is fixed.

In this embodiment, the clamping piece includes a clamping block and a clamping groove, the clamping groove is arranged on the outer shoulder, the clamping block is arranged on the inner shoulder, and further sealing is realized through the matching of the clamping block and the clamping groove.

In the present embodiment, a sealing ring 17 is connected to the interface of the inner shoulder and the outer shoulder.

In this embodiment, during the use, at first plant in planting the device plant one or two plants after pass through the draw-in groove with it and transparent cage and be connected, then connect gas exchange and collection device, nutrition adding device and liquid collection device, then add the nutrient solution and cultivate, after the plant normally grows, can carry out root secretion and collect. After culturing for a period of time, the volatile gas of the plants can be collected by gas exchange and the gas in the adsorption column adsorption device. The utility model adds nutrient solution by using nutrition, collects and extracts root exudates by the liquid collecting device after plants are absorbed and utilized, collects plant volatile gas by the gas exchanging and collecting device, has good isolation between the culture device and the outside, can ensure that the root exudates extract and the plant volatile gas collector relatively accord with an ideal state, has no pollution of other substances, has good capability of continuously collecting sample liquid by the liquid collecting device, has good capability of collecting volatile gas by the gas exchanging and collecting device, is simple and easy to operate, and is convenient to carry.

Any embodiment disclosed herein above is meant to disclose, unless otherwise indicated, all numerical ranges disclosed as being preferred, and any person skilled in the art would understand that: the preferred ranges are merely those values which are obvious or representative of the technical effect which can be achieved. Since the numerical values are too numerous to be exhaustive, some of the numerical values are disclosed in the present invention to illustrate the technical solutions of the present invention, and the above-mentioned numerical values should not be construed as limiting the scope of the present invention.

If the terms "first," "second," etc. are used herein to define parts, those skilled in the art will recognize that: the terms "first" and "second" are used merely to distinguish one element from another in a descriptive sense and are not intended to have a special meaning unless otherwise stated.

If the utility model discloses or relates to parts or structures which are fixedly connected to each other, the fixedly connected parts can be understood as follows, unless otherwise stated: a detachable fixed connection (for example using bolts or screws) is also understood as: non-detachable fixed connections (e.g. riveting, welding), but of course, fixed connections to each other may also be replaced by one-piece structures (e.g. manufactured integrally using a casting process) (unless it is obviously impossible to use an integral forming process).

In addition, the orientations or positional relationships indicated by the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like used in any of the embodiments disclosed in the present invention are based on the orientations or positional relationships shown in the drawings, and are only for convenience of describing the present patent, rather than to indicate or imply that the device or element so referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be interpreted as limiting the patent, and the terms used to describe shapes in any of the above-described embodiments of the present disclosure are intended to include shapes that are similar, analogous, or approximate thereto, unless otherwise indicated, and the adsorbent column packing includes but is not limited to polydivinylbenzene porous pellet GDX-101.

Any part provided by the utility model can be assembled by a plurality of independent components or can be manufactured by an integral forming process.

Finally, it should be noted that the above examples are only used to illustrate the technical solutions of the present invention and not to limit the same; although the present invention has been described in detail with reference to preferred embodiments, those skilled in the art will understand that: modifications to the specific embodiments of the utility model or equivalent substitutions for parts of the technical features may be made; without departing from the spirit of the present invention, it is intended to cover all aspects of the utility model as defined by the appended claims.

Claims

1. a plant root exudate and plant volatile gas are continuously collected and cultivating device, it is characterized in that: comprise cultivating device, the upper part of culturing device is connected with gas exchange and collecting device through pipeline, and the middle part of culturing device is connected with nutrient adding device through pipeline The bottom of the culture device is connected with a liquid collection device through a pipeline, and the inner bottom of the culture device is provided with vermiculite as a culture medium.

2. The plant root exudate and plant volatile gas continuous collection and cultivation device according to claim 1, characterized in that: the cultivation device comprises a petri dish, the petri dish is clamped with an isolation cover, and the vermiculite is stacked on the cultivation dish. On the dish, a liquid interface is provided on the side wall of the bottom of the culture dish.

3. The plant root exudate and plant volatile gas continuous collection and cultivation device according to claim 2, characterized in that: a nutrient solution interface is provided on the side wall of the bottom of the isolation cover, and a nutrient solution interface is provided on the side wall of the middle part of the isolation cover. At least two gas exchange interfaces are provided, one of which is connected to the gas exchange and collection device through a pipeline, and a volatile gas adsorption column is connected to the pipeline.

4 . The device for continuously collecting and cultivating plant root exudates and plant volatile gases according to claim 3 , wherein the gas exchange interfaces are covered with filter membranes. 5 .

5 . The device for continuously collecting and cultivating plant root exudates and plant volatile gases according to claim 1 , wherein the gas exchange and collection device is a vacuum pump. 6 .

6 . The device for continuously collecting and cultivating plant root exudates and plant volatile gases according to claim 2 , wherein the connection between the culture dish and the isolation cover is sealed by a parafilm. 7 .

7. The device for continuously collecting and cultivating plant root exudates and plant volatile gas according to claim 3, wherein the nutrient adding device comprises an infusion bottle, the infusion bottle is filled with nutrient solution, and the nutrient solution interface is filled with Base rubber stopper, a medical infusion set is connected under the infusion bottle, one end of the medical infusion set is connected to the output end at the bottom of the infusion bottle, and the other end passes through the butyl rubber stopper of the nutrient solution interface, and extends into the isolation cover to drop the nutrients into the device middle.

8. The plant root exudate and plant volatile gas continuous collection and cultivation device according to claim 3, is characterized in that: described liquid interface is filled with butyl rubber stopper, and described liquid collection device comprises sampling bottle, sampling The bottle is connected to the liquid interface through a liquid receiving pipe with an on-off valve, one end of the liquid receiving pipe is connected to the input end of the sampling bottle, and the other end passes through the butyl rubber stopper of the liquid interface and extends into the petri dish.

9. The plant root exudate and plant volatile gas continuous collection and cultivation device according to claim 6 is characterized in that: the inner edge of the upper end interface of the culture dish is provided with an upward inner shoulder, and the The outer edge of the interface at the lower end of the isolation cover is provided with an outer convex shoulder that is downwardly matched with the inner convex shoulder, and the abutting surface of the inner convex shoulder and the outer convex shoulder is provided with a mutual-matching clip, through the inner convex shoulder, The cooperation of the outer convex shoulders realizes the clamping and fixing of the culture dish and the isolation cover.

10 . The device for continuously collecting and cultivating plant root exudates and plant volatile gases according to claim 9 , wherein the clipping member comprises a clipping block and a clipping slot, and the clipping slot is arranged on the outer shoulder. 11 . , the clamping block is arranged on the inner convex shoulder.