CN214794576U - Reference electrode based on micro-fluidic technology - Google Patents
Reference electrode based on micro-fluidic technology Download PDFInfo
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- CN214794576U CN214794576U CN202121042969.7U CN202121042969U CN214794576U CN 214794576 U CN214794576 U CN 214794576U CN 202121042969 U CN202121042969 U CN 202121042969U CN 214794576 U CN214794576 U CN 214794576U
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- reference electrode
- liquid
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- main liquid
- silver
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- 238000005516 engineering process Methods 0.000 title claims abstract description 15
- 239000007788 liquid Substances 0.000 claims abstract description 73
- 229910021607 Silver chloride Inorganic materials 0.000 claims description 15
- 229910052709 silver Inorganic materials 0.000 claims description 15
- 239000004332 silver Substances 0.000 claims description 15
- HKZLPVFGJNLROG-UHFFFAOYSA-M silver monochloride Chemical compound [Cl-].[Ag+] HKZLPVFGJNLROG-UHFFFAOYSA-M 0.000 claims description 15
- 238000012360 testing method Methods 0.000 claims description 11
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 5
- 230000000694 effects Effects 0.000 abstract description 3
- 238000005259 measurement Methods 0.000 abstract description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 description 11
- 239000012482 calibration solution Substances 0.000 description 11
- 238000000926 separation method Methods 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- 238000000840 electrochemical analysis Methods 0.000 description 4
- 239000000243 solution Substances 0.000 description 4
- 230000004913 activation Effects 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000000835 electrochemical detection Methods 0.000 description 2
- 239000003792 electrolyte Substances 0.000 description 2
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 2
- 229910052753 mercury Inorganic materials 0.000 description 2
- 238000012123 point-of-care testing Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 239000000017 hydrogel Substances 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 239000012088 reference solution Substances 0.000 description 1
- 229920006395 saturated elastomer Polymers 0.000 description 1
- 238000007650 screen-printing Methods 0.000 description 1
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Abstract
The utility model discloses a reference electrode based on micro-fluidic technology relates to the reference electrode field. The device comprises a main liquid path and a liquid separating path communicated with the main liquid path, wherein a reference electrode is arranged in the liquid separating path, an air vent is arranged at the tail end of the liquid separating path, and an overflow-preventing filter element is arranged in the air vent. Calibration liquid in the main liquid way flows to dividing the interior of liquid way simultaneously, divides the terminal overflow-preventing filter core of liquid way to meet water and then seals airtight impervious, and the calibration liquid stops flowing, can prevent moreover that the outflow card from getting into the instrument. The problem that the reference electrode is complex to manufacture in the prior art is solved. The effects of simple manufacture of the reference electrode and accurate potential measurement are realized.
Description
Technical Field
The utility model relates to a reference electrode field especially relates to a reference electrode based on micro-fluidic technique.
Background
In electrochemical detection, a reference electrode is required to provide a stable potential reference. The traditional reference electrode is made of cylindrical glass or plastic material and has large volume, and the silver/silver chloride or mercury/mercury chloride in the reference electrode is soaked in a saturated or certain-concentration chloride ion solution to provide stable potential. The test card used for the flat electrochemical sensor, particularly for the POCT point-of-care test, requires miniaturization of the reference electrode. US4933048A provides a miniaturized reference electrode with the silver/silver chloride reference electrode and the detection electrode in the same fluid path. And the reference electrode is sequentially coated with a hydrogel layer and a semipermeable membrane which is permeable to water and impermeable to chloride ions. During the period of detection by the test card, the change of the chloride ion concentration in the calibration solution or the sample does not cause the potential fluctuation of the reference electrode. However, the manufacturing process is cumbersome and requires two films of different compositions to be applied to the silver/silver chloride.
SUMMERY OF THE UTILITY MODEL
The utility model aims at providing a reference electrode based on micro-fluidic technology to solve the complicated problem of reference electrode preparation among the prior art. The effects of simple manufacture of the reference electrode and accurate potential measurement are realized.
In order to realize the effect, the utility model discloses a reference electrode based on micro-fluidic technology, including main liquid way and rather than communicating branch liquid way, divide and be equipped with reference electrode in the liquid way, divide the terminal bleeder vent that is equipped with in the liquid way, be equipped with in the bleeder vent and end the excessive filter core. Calibration liquid in the main liquid way flows to dividing the interior of liquid way simultaneously, divides the terminal overflow-preventing filter core of liquid way to meet water and then seals airtight impervious, and the calibration liquid stops flowing, can prevent moreover that the outflow card from getting into the instrument.
Furthermore, a plurality of test electrodes are arranged in the main liquid path.
Furthermore, the distance between the position of the reference electrode and the junction of the main liquid path and the liquid separating path is 0.2-5 cm. The reference electrode has a proper distance with the junction of the main liquid path and the liquid separating path, so that the influence of the liquid connecting potential can be eliminated.
Further, the reference electrode is a silver/silver chloride electrode. The calibration solution in the liquid dividing path contains chloride ions, and the silver/silver chloride can provide a potential reference after contacting with the calibration solution, so that the electrochemical test is completed.
The beneficial effects of the utility model reside in that:
1. the reference electrode is silver/silver chloride, and a film with other components is not required to be coated, so that the reference electrode is simple to manufacture.
2. The overflow-preventing filter element and the microfluidic technology enable the calibration solution for electrode activation to simultaneously provide chloride ions for the reference electrode without additional reference solution.
3. The reference electrode based on the microfluidic technology can provide stable potential for electrochemical tests.
Drawings
Fig. 1 is a schematic diagram of a microfluidic reference electrode provided in an embodiment of the present application.
Fig. 2 is a graph of the potential change of the microfluidic reference electrode and the commercial reference electrode in the calibration solution and the test sample.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the drawings in the embodiments of the present invention are combined below to clearly and completely describe the technical solutions in the embodiments of the present invention. It is to be understood that the embodiments described are only some of the embodiments of the present invention, and not all of them. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
As shown in fig. 1, the reference electrode based on the microfluidic technology comprises a main liquid path and a liquid separating path communicated with the main liquid path, wherein the reference electrode is arranged in the liquid separating path, the tail end of the liquid separating path is provided with an air hole, and an anti-overflow filter element is arranged in the air hole. Calibration liquid in the main liquid way flows to dividing the interior of liquid way simultaneously, divides the terminal overflow-preventing filter core of liquid way to meet water and then seals airtight impervious, and the calibration liquid stops flowing, can prevent moreover that the outflow card from getting into the instrument. A plurality of test electrodes are arranged in the main liquid path. The distance between the position of the reference electrode and the junction of the main liquid path and the liquid separating path is 0.2-5 cm. The reference electrode has a proper distance with the junction of the main liquid path and the liquid separating path, so that the influence of the liquid connecting potential can be eliminated. The reference electrode is a silver/silver chloride electrode. The calibration solution in the liquid dividing path contains chloride ions, and the silver/silver chloride can provide a potential reference after contacting with the calibration solution, so that the electrochemical test is completed.
In particular, the test card can be used for testing blood gas, electrolyte, biochemical or immunological items, the sensor assembly is manufactured by adopting a printed circuit board technology, a flexible circuit board technology or a thick film screen printing technology, and the reference electrode is silver/silver chloride. A plurality of test electrodes are distributed in a main liquid path 1, and a liquid dividing path 2 is arranged at a proper position of the main liquid path. The reference electrode 3 is positioned in the liquid separation path 2, and the distance between the reference electrode and the junction of the main liquid path and the liquid separation path is 0.2-5 cm. The tail end of the liquid distribution path is provided with a breather hole 4, and an overflow-preventing filter element 5 is arranged in the breather hole. When the card is used, electrode activation is needed firstly, and the calibration liquid flows in the main liquid path 1 and flows to the liquid separating path 2. The calibration liquid in the liquid separation path discharges air through the overflow-proof filter element 5, and contacts the overflow-proof filter element 5 when the calibration liquid flows to the tail end air vent 4. The overflow-preventing filter element is sealed, airtight and waterproof after meeting water, and the calibration liquid stops flowing. The calibration solution in the liquid dividing path does not flow in the electrode activation and sample detection processes, and the silver/silver chloride electrode in the liquid dividing path is soaked in the calibration solution containing chloride ions and can be used as a reference electrode to provide stable potential. The distance between the reference electrode in the liquid separation circuit and the junction of the main circuit/the liquid separation circuit eliminates the influence of the diffusion of chloride ions and other components, reduces the liquid contact potential in the process of testing a sample, and can provide a potential reference for electrochemical detection.
As shown in fig. 2, a commercial reference electrode was immersed in a microchannel to test the potential change of the silver/silver chloride reference electrode in different solutions. And (3) injecting a calibration solution with chloride ion concentration of 100mM into the liquid path, and flowing the calibration solution into the liquid dividing path to cover the silver/silver chloride electrode sites. The potential of the silver/silver chloride reference electrode is reduced due to hydration and the like, and the potential is basically not reduced for about 90s and is kept unchanged. When the whole blood sample with the chloride ion concentration of 150mM is injected in about 180s, the potential of the silver/silver chloride reference electrode is basically kept unchanged, and the potential reference can be provided for detection cards of blood gas, electrolyte, biochemistry, immunity and the like, so that the electrochemical test is completed.
The above-mentioned embodiments are only intended to describe the preferred embodiments of the present invention, but not to limit the scope of the present invention, and various modifications and improvements made by those skilled in the art without departing from the spirit of the present invention should fall within the protection scope defined by the claims of the present invention.
Claims (4)
1. A reference electrode based on micro-fluidic technology, characterized in that: the liquid level control device comprises a main liquid path (1) and a liquid dividing path (2) communicated with the main liquid path, wherein a reference electrode (3) is arranged in the liquid dividing path, an air vent (4) is arranged at the tail end of the liquid dividing path, and an overflow-preventing filter element (5) is arranged in the air vent.
2. A reference electrode based on microfluidic technology according to claim 1, characterized in that: a plurality of test electrodes (6) are arranged in the main liquid path.
3. A reference electrode based on microfluidic technology according to claim 1, characterized in that: the distance between the position of the reference electrode and the junction of the main liquid path and the liquid separating path is 0.2-5 cm.
4. A reference electrode based on microfluidic technology according to claim 1, characterized in that: the reference electrode is a silver/silver chloride electrode.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121042969.7U CN214794576U (en) | 2021-05-17 | 2021-05-17 | Reference electrode based on micro-fluidic technology |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121042969.7U CN214794576U (en) | 2021-05-17 | 2021-05-17 | Reference electrode based on micro-fluidic technology |
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| Publication Number | Publication Date |
|---|---|
| CN214794576U true CN214794576U (en) | 2021-11-19 |
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| CN202121042969.7U Active CN214794576U (en) | 2021-05-17 | 2021-05-17 | Reference electrode based on micro-fluidic technology |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113219029A (en) * | 2021-05-17 | 2021-08-06 | 山东卓越生物技术股份有限公司 | Reference electrode based on micro-fluidic technology |
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2021
- 2021-05-17 CN CN202121042969.7U patent/CN214794576U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113219029A (en) * | 2021-05-17 | 2021-08-06 | 山东卓越生物技术股份有限公司 | Reference electrode based on micro-fluidic technology |
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