CN212393701U

CN212393701U - Isolated Liver Perfusion System and Connector Tube Assembly

Info

Publication number: CN212393701U
Application number: CN202021707325.0U
Authority: CN
Inventors: 吴懿苗; 梁铭炬; 杨树进; 许晓珊; 周伟津; 邝伟健; 何锡然; 陈素平; 郭家钘
Original assignee: Guangdong Shunde Industrial Design Institute
Current assignee: Guangdong Shunde Industrial Design Institute
Priority date: 2020-08-17
Filing date: 2020-08-17
Publication date: 2021-01-26
Anticipated expiration: 2030-08-17

Abstract

The utility model relates to an isolated liver perfusion system and a joint pipe assembly. The joint pipe assembly comprises an intubation seat and a docking seat. The cannula seat is provided with a first pressing end face, and the through hole is located in the middle of the first pressing end face. The docking seat is provided with a docking hole, and the docking hole and the position of the through hole are arranged correspondingly. The docking seat is also provided with a second pressing end face. The butting hole is located in the middle of the second pressing end face, and the second pressing end face is press-fitted with the first pressing end face. The docking seat is further provided with a connecting pipe which communicates with the docking hole, and the connecting pipe is used for communicating with the pipeline of consumables. In this way, there is no contact with the inner wall of the portal vein or the proper liver artery during the perfusion process, which avoids damage to the endothelial tissue of the portal vein or the proper liver artery, and does not require suture operation, which greatly improves the work efficiency of assembly. In addition, The connector tube assembly is firmly attached to the artery or portal vein of the isolated liver, and there is no risk of detachment during perfusion.

Description

In vitro liver perfusion system and joint pipe assembly

Technical Field

The utility model relates to a fill technical field, especially relate to an separation liver perfusion system and joint pipe subassembly.

Background

Liver transplantation is the only effective means for treating end-stage liver diseases, and because organs are in short supply, a plurality of end-stage liver disease patients and the like die due to not being transplanted with proper liver sources, marginal liver supply transplantation has great significance for the field of liver transplantation. The peripheral liver supply has poor quality, the damage of the peripheral liver supply can be further aggravated by the traditional static cold storage, and the quality of the peripheral liver supply can be effectively improved by the Mechanical Perfusion (MP) technology of the isolated liver, so that the number of transplantable livers is increased.

The mechanical perfusion of the isolated liver is a mode for preserving and transferring organs, the liver is obtained and then is connected with a self blood vessel to a mechanical perfusion system of the isolated liver, and the mechanical perfusion system of the isolated liver continuously perfuses perfusion liquid to the liver at the liver preservation and transfer stage and simultaneously supplies oxygen, nutrients and the like to the liver. The liver storage device is a core part of an in-vitro liver mechanical perfusion system, and when perfusion is carried out, the liver is placed in the liver storage device and is connected to a circulating pipeline through a cannula and a connector on the liver storage device. The perfusion mode of the liver in the mechanical perfusion system can be divided into two modes according to the pipeline connection mode: firstly, closed-loop perfusion is carried out, namely a consumable pipeline is connected with hepatic artery and portal vein of the liver through an intubation tube and is used as a perfusion liquid to enter a liquid inlet of the liver; the lower vena cava of the liver is connected to the consumable pipeline through the cannula, and the lower vena cava is used as a liquid outlet of the liver as perfusate to form a closed circulating pipeline. Secondly, open-loop perfusion, namely a consumable pipeline is connected with hepatic artery and portal vein of the liver through an intubation tube and used as a perfusion liquid to enter a liquid inlet of the liver; the inferior vena cava of the liver is not connected with any cannula, perfusate directly flows out of the liver storage device from the inferior vena cava after flowing through the liver, and is discharged into a consumable pipeline from a liquid outlet of the liver storage device to form an open-loop circulation pipeline.

During perfusion, the liver storage device is used as an intermediate carrier to connect the liver to the circulating pipeline for circulating perfusion, so that the design of the liver storage device meets the requirements that the liver is comfortably perfused and can be perfectly connected to the circulating pipeline for perfusion. The artery intubation or portal intubation of the liver is a medium for connecting the liver to a circulation pipeline, is usually in a long tubular shape, one end of the intubation is connected to the pipeline during perfusion, the other end of the intubation is inserted into the portal vein or venous tube of the liver, and the perfusion is performed by fastening and fixing the intubation by using an operation line.

SUMMERY OF THE UTILITY MODEL

In view of the above, there is a need to overcome the drawbacks of the prior art and to provide an extracorporeal liver perfusion system and connector tube assembly, which can facilitate assembly and connection and avoid damage to the endothelial tissue of the portal vein of the extracorporeal liver or the intrinsic artery of the liver.

The technical scheme is as follows: a joint tube assembly, comprising:

the intubation tube seat is provided with a through hole for inserting an artery or a portal vein of an in-vitro liver, the intubation tube seat is provided with a first compression end face, and the through hole is positioned in the middle of the first compression end face;

the butt joint seat, the butt joint seat is equipped with the butt joint hole, the butt joint hole with the corresponding setting in through-hole position, the butt joint seat still is equipped with the second and compresses tightly the terminal surface, the butt joint hole is located the second compresses tightly the middle part of terminal surface, the second compress tightly the terminal surface with the first terminal surface that compresses tightly the cooperation, the butt joint seat still be equipped with the connecting pipe that the butt joint hole is linked together, the connecting pipe is used for being linked together with the consumptive material pipeline.

The connector tube assembly can connect the artery or portal vein of the isolated liver with the consumable pipeline, so that the artery or portal vein of the isolated liver is connected into the perfusion circulation pipeline. The artery and the connection process of the connector pipe assembly are explained by taking the artery of the isolated liver and the consumable pipeline as an example, the trunk at the tail end of the artery is reserved before perfusion, and the trunk at the tail end of the artery is cut into sheets circumferentially arranged around the tail end of the artery, the artery is inserted into the through hole, the sheets at the tail end of the artery are flatly paved on a first compression end face, the first compression end face of the butt joint seat is compressed and matched with the second compression end face of the cannula seat, the butt joint hole is communicated with the artery at the moment, in addition, the connecting pipe is communicated with the consumable pipeline, and the artery of the isolated liver is communicated with the consumable pipeline. The isolated liver can start to be perfused after being connected into the perfusion circulating pipeline, and after the perfusion is finished, the butt joint seat and the cannula seat are separated, the sheet at the tail end of the artery is trimmed off, and the perfusion is finished. Therefore, the cannula is not directly inserted into the artery as the traditional method, so that the inner wall of the artery vessel is not contacted in the perfusion process, the damage to the endothelial tissue of the artery is avoided, meanwhile, the suture operation is not needed, the assembly working efficiency is greatly improved, in addition, the firmness degree of the combination of the joint tube assembly and the artery or portal vein of the isolated liver is higher, and the shedding risk cannot occur in the perfusion process.

In one embodiment, one side of the plug socket is movably connected with one side of the butt-joint socket, and the other side of the plug socket is detachably connected with the other side of the butt-joint socket through a mounting piece.

In one embodiment, two U-shaped connecting plates are arranged on one side of the socket at intervals, a connecting rod and a rotating shaft connected with the connecting rod are arranged on one side of the butt joint seat, one end of the rotating shaft is rotatably arranged in one of the U-shaped connecting plates and can move along one of the U-shaped connecting plates, the other end of the rotating shaft is rotatably arranged in the other U-shaped connecting plate and can move along the other U-shaped connecting plate, and the connecting rod is movably arranged in the interval between the two U-shaped connecting plates; and a screw hole is formed in the other side part of the socket, a mounting hole corresponding to the screw hole is formed in the other side part of the butt joint seat, and the mounting part is a screw corresponding to the screw hole.

In one embodiment, the first pressing end surface is a plane, and the second pressing end surface is a plane.

In one embodiment, the connector assembly further includes a cannula connector disposed between the connection tube and the consumable pipeline, the cannula connector is configured to be mounted on a wall of the liver storage device, and the connection tube is connected to the consumable pipeline through the cannula connector.

In one embodiment, the docking station is provided with a lateral port luer connector, and the lateral port luer connector is positioned on the side wall of the connecting tube and is communicated with the connecting tube.

An in-vitro liver perfusion system comprises two connector tube assemblies, a liver storage device and a consumable pipeline; the bottom of the liver storage device is used for installing perfusate, the liver storage device is also used for placing the isolated liver, one of the connector tube assemblies is used for being communicated with the artery of the isolated liver, the other connector tube assembly is used for being communicated with the portal vein of the isolated liver, and the connecting tube is communicated with the consumable pipeline.

The in-vitro liver perfusion system comprises the joint pipe assembly, so that the technical effect is brought by the joint pipe assembly, and the beneficial effects are the same as those of the joint pipe assembly and are not repeated herein.

In one embodiment, the liver storage device is further provided with an openable cover body, and the connector pipe assembly is positioned inside the liver storage device; store up liver ware include the bottom plate and with the curb plate that the bottom plate links to each other, the curb plate winds bottom plate circumference sets up, the curb plate is the ladder board, the opening size of curb plate is diminished by top to bottom.

In one embodiment, the liver storage device comprises a bottom plate, the bottom plate is a concave plate, a liquid guide groove is arranged at the bottom part of the concave plate, a liquid outlet is arranged on the bottom wall of the liquid guide groove, the bottom wall of the liquid guide groove is an inclined wall which is obliquely arranged, the liquid outlet is arranged at the lower part of the inclined wall, and a liquid outlet joint is arranged at the liquid outlet.

In one embodiment, the concave surface of the concave plate is a semi-ellipsoidal surface or a semi-hemispherical surface.

Drawings

The accompanying drawings, which are incorporated in and constitute a part of this application, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification.

In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings needed to be used in the description of the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art to obtain other drawings without creative efforts.

Fig. 1 is a schematic structural view of a connector tube assembly according to an embodiment of the present invention in combination with an extracorporeal liver;

fig. 2 is a schematic structural view of a joint pipe assembly according to an embodiment of the present invention;

fig. 3 is a schematic structural view of a junction block assembly according to an embodiment of the present invention;

fig. 4 is a schematic structural view illustrating a closed cover of a liver storage device according to an embodiment of the present invention;

fig. 5 is a schematic structural view of one of the viewing angles of the liver storage device according to an embodiment of the present invention after the cover is removed;

fig. 6 is a schematic structural view of another view angle of the liver storage device according to an embodiment of the present invention after the cover is removed;

fig. 7 is a schematic structural view of a liver storage device according to an embodiment of the present invention with a cover removed;

FIG. 8 is a schematic view of the arrangement of FIG. 7 with an internal bladder disposed therein;

FIG. 9 is a schematic view of a perspective view of a liver in vitro positioned over the balloon of FIG. 8;

FIG. 10 is a schematic view of another perspective of placing a liver ex vivo over the balloon of FIG. 8;

FIG. 11 is a schematic diagram of the placement of a moisture-retaining membrane over the excised liver of FIG. 10;

fig. 12 is a schematic structural diagram of an extracorporeal liver perfusion system according to an embodiment of the present invention.

10. A junction block assembly; 11. a cannula holder; 111. a through hole; 112. a first compression end face; 113. a screw hole; 114. a U-shaped connecting plate; 1141. an anti-drop flange; 12. a docking station; 121. a second compression end face; 122. a connecting pipe; 123. mounting holes; 124. a mounting member; 125. a side hole luer fitting; 126. a connecting rod; 127. a rotating shaft; 20. liver in vitro; 21. an artery; 22. a sheet material; 30. a liver storage device; 31. a cover body; 311. a fastener; 32. a base plate; 321. a liquid guide groove; 322. a liquid outlet; 323. a liquid outlet joint; 324. reinforcing rib plates; 33. a side plate; 331. a front side plate; 332. a rear side plate; 333. a left side plate; 334. a right side plate; 341. 342, 343, 344, 345, a through plate joint; 35. a back-off structure; 40. a power pump; 50. a membrane lung; 61. an air-oxygen mixer; 62. an oxygen tank; 63. a carbon dioxide tank; 64. a heat exchange device; 71. a microembolus filter; 72. a pressure sensor; 73. a flow sensor; 74. a host; 75. a display screen; 76. a power source; 77. a bile-containing device; 81. an air bag; 82. a moisture retention film.

Detailed Description

In order to make the above objects, features and advantages of the present invention more comprehensible, embodiments of the present invention are described in detail below with reference to the accompanying drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may, however, be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, as those skilled in the art will be able to make similar modifications without departing from the spirit and scope of the present invention.

Generally, the conventional insertion tube corresponding to the hepatic artery and the insertion tube corresponding to the portal vein are usually in a tube shape, after the insertion tubes are inserted into the hepatic artery or the portal vein of the liver, medical staff still need to tie and fix the insertion tubes by using an operation line, the operation is complicated, the surgical line is not tied tightly, the risk that the insertion tubes fall off exists in the perfusion process, and the endothelial tissue of the portal vein or the arterial blood vessel can be damaged in the process that the insertion tubes are inserted into the hepatic artery or the portal vein.

Based on this, referring to fig. 1 to 3, fig. 1 shows a schematic structural diagram of a joint pipe assembly 10 and an isolated liver 20 of an embodiment of the present invention, fig. 2 shows a schematic structural diagram of a joint pipe assembly 10 of an embodiment of the present invention, and fig. 3 shows a schematic structural diagram of a joint pipe assembly 10 of an embodiment of the present invention. An embodiment of the utility model provides a pair of joint pipe assembly 10, joint pipe assembly 10 includes: a socket 11 and a docking socket 12.

The cannula holder 11 is provided with a through hole 111 for inserting the artery 21 or the portal vein of the isolated liver 20, the cannula holder 11 is provided with a first pressing end surface 112, and the through hole 111 is positioned in the middle of the first pressing end surface 112. The docking seat 12 is provided with a docking hole, and the docking hole is arranged corresponding to the through hole 111. The docking cradle 12 is further provided with a second compression end face 121. The butt joint hole is located in the middle of the second pressing end face 121, and the second pressing end face 121 is in press fit with the first pressing end face 112. The docking seat 12 is further provided with a connection tube 122 communicated with the docking hole, and the connection tube 122 is used for being communicated with a consumable pipeline.

The connector tube assembly 10 described above is capable of connecting the artery 21 or portal of the extracorporeal liver 20 to a consumable line such that the artery 21 or portal of the extracorporeal liver 20 is connected to a perfusion circuit. The process of connecting the artery 21 and the connector tube assembly 10 is described by taking the connection of the artery 21 of the isolated liver 20 and the consumable pipeline as an example, the trunk at the end of the artery 21 is reserved before perfusion, the trunk at the end of the artery 21 is cut into sheets 22 circumferentially arranged around the end of the artery 21, the artery 21 is inserted into the through hole 111, the sheets 22 at the end of the artery 21 are flatly laid on the first pressing end face 112, the first pressing end face 112 of the butt joint seat 12 is in pressing fit with the second pressing end face 121 of the cannula seat 11, at the moment, the butt joint hole is communicated with the artery 21, in addition, the connecting tube 122 is communicated with the consumable pipeline, and the communication between the artery 21 of the isolated liver 20 and the consumable pipeline is realized. The isolated liver 20 is connected to the perfusion circulation pipeline, and then the perfusion operation is started, after the perfusion is finished, the butt joint seat 12 and the cannula seat 11 are separated, the sheet 22 at the tail end of the artery 21 is trimmed, and the perfusion is finished. It can be seen that, instead of inserting the cannula directly into the artery 21 as in the conventional method, the cannula does not contact the inner wall of the artery 21 during the perfusion process, so that the injury to the endothelial tissue of the artery 21 is avoided, and meanwhile, the suture operation is not required, so that the working efficiency of assembly is greatly improved, and in addition, the firmness of the combination of the connector tube assembly 10 and the artery 21 or portal vein of the isolated liver 20 is higher, so that the risk of falling off during the perfusion process is avoided.

Referring to fig. 1 to 3, further, one side of the cannula holder 11 is movably connected to one side of the docking holder 12, and the other side of the cannula holder 11 is detachably connected to the other side of the docking holder 12 through the mounting member 124. Thus, since one side of the socket 11 is movably connected to one side of the docking cradle 12, when the socket 11 and the docking cradle 12 are opened and closed, the other side of the socket 11 and the other side of the docking cradle 12 are assembled together by the mounting member 124, which facilitates the opening and closing operation between the socket 11 and the docking cradle 12.

It should be noted that "movable" in "movable" connection in this embodiment is referred to as "fixed", and when two components are "movable" connected, it should be understood that two components are not "fixed" connected, but are, for example: rotationally, and/or movably in either direction, etc.

Specifically, one side of the docking pod 12 can not only move relative to one side of the socket 11 in a direction perpendicular to the first compression end surface 112, but one side of the docking pod 12 can also rotate relative to one side of the socket 11. Thus, when the docking base 12 and the cannula base 11 are in the open state, since one side of the docking base 12 can move in the direction perpendicular to the first pressing end surface 112 relative to one side of the cannula base 11, the first pressing end surface 112 and the second pressing end surface 121 are located at relatively separated positions, so that the first pressing end surface 112 and the second pressing end surface 121 can better fix the sheet 22 at the end of the artery 21.

In one embodiment, referring to fig. 3, two U-shaped connecting plates 114 are spaced apart from each other on one side of the socket 11, a connecting rod 126 and a rotating shaft 127 connected to the connecting rod 126 are disposed on one side of the docking station 12, one end of the rotating shaft 127 is rotatably disposed in one of the U-shaped connecting plates 114 and can move along one of the U-shaped connecting plates 114, the other end of the rotating shaft 127 is rotatably disposed in the other U-shaped connecting plate 114 and can move along the other U-shaped connecting plate 114, and the connecting rod 126 is movably disposed in the space between the two U-shaped connecting plates 114.

Further, a retaining flange 1141 is provided at a mouth portion of the U-shaped connecting plate 114. The anti-slip flange 1141 is used for spacing interference fit with the rotation shaft 127 to prevent the rotation shaft 127 from moving outward from the mouth of the U-shaped connecting plate 114. The U-shaped connecting plate 114 is made of an elastic material, so that the rotating shaft 127 can be moved out from the mouth of the U-shaped connecting plate 114 under the action of a force applied to the rotating shaft 127, that is, the socket 11 is separated from the docking cradle 12 for cleaning and maintenance. The assembly of the cannula holder 11 with the docking holder 12 can also be facilitated by reversing the operating steps.

It will be appreciated that, in order to achieve the effect that one side of the docking station 12 can move relative to one side of the socket 11 in a direction perpendicular to the first pressing end surface 112 and can also rotate relative to one side of the socket 11, the above-mentioned U-shaped connecting plate 114, connecting rod 126 and rotating shaft 127 are not limited to be used, and the manner of matching one side of the socket 11 with one side of the docking station 12 can also be other designs, which are not limited herein. For example, one side of the socket 11 is provided with a guide rail, and one side of the docking socket 12 is provided with a slider engaged with the guide rail, the slider being slidably disposed on the guide rail, thereby enabling the docking socket 12 to move relative to the socket 11. For another example, when the rotating shaft 127 is rotatably connected to the connecting rod 126, the docking cradle 12 can be rotatably closed on the socket 11.

Referring to fig. 1 to 3, a screw hole 113 is formed on the other side of the cannula holder 11, a mounting hole 123 corresponding to the screw hole 113 is formed on the other side of the docking holder 12, and the mounting member 124 is a screw rod corresponding to the screw hole 113.

In an alternative embodiment, instead of a combination of rotatably connecting one side of the cannula holder 11 and the docking holder 12 and detachably connecting the other side of the cannula holder 11 and the docking holder 12, the cannula holder 11 is integrally detachably connected to the docking holder 12, and specifically, the connection between the cannula holder 11 and the docking holder 12 may be, for example, a snap connection, a rivet connection, a screw connection, a bolt connection, a pin connection, and the like, which is not limited herein.

Referring to fig. 1 to 3, in one embodiment, the first pressing end surface 112 is a plane, and the second pressing end surface 121 is a plane. Thus, when the first pressing end surface 112 and the second pressing end surface 121 are in pressing fit, the sheet 22 at the end of the artery 21 can be firmly pressed, and the sealing performance is ensured. Alternatively, the first pressing end surface 112 may also be an arc-shaped surface, and the second pressing end surface 121 is an arc-shaped surface corresponding to the first pressing end surface 112. The first pressing end surface 112 may be a surface having another shape, which is not limited herein, as long as the second pressing end surface 121 is adapted to the shape of the first pressing end surface 112, so that the sheet 22 at the end of the artery 21 can be firmly pressed when the first pressing end surface 112 and the second pressing end surface 121 are in pressing fit.

Referring to fig. 1-3, in one embodiment, the connector tube assembly 10 further includes a cannula connector (specifically, two threading connectors 341 shown in fig. 4) disposed between the connection tube 122 and the consumable tubing. The intubation joint is used for being installed on the wall of the liver storage device 30, and the connection tube 122 is connected with the consumable pipeline through the intubation joint. In this way, it is possible to facilitate the connection of the connection tube 122 inside the liver storage 30 to the consumable line outside the liver storage 30.

Referring to fig. 1-3, in one embodiment, the docking station 12 is provided with a lateral port luer 125. A lateral bore luer fitting 125 is located on the sidewall of the connecting tube 122 and communicates with the connecting tube 122. Therefore, the pressure pipe can be directly connected to the lateral hole luer connector 125, the position of the pressure connector is arranged on the docking seat 12, the distance between the pressure connector and the portal vein or artery 21 is very close, the pressure of the portal vein or artery 21 can be measured more truly, and the phenomenon that the pressure pipe is connected from the middle of the circulating pipeline through the connector and is far away from the portal vein or artery 21, pressure difference is generated, and large measurement errors are caused is avoided.

In one embodiment, a method of connecting a fitting tube assembly 10 according to any of the above embodiments, comprises the steps of:

when the connector tube assembly 10 is connected with the artery 21 of the excised liver 20, the trunk at the tail end of the artery 21 is cut into sheets 22 which are circumferentially arranged around the tail end of the artery 21, the artery 21 is inserted into the through hole 111, the sheets 22 at the tail end of the artery 21 are flatly paved on the first pressing end surface 112, and the first pressing end surface 112 of the butt joint seat 12 is in pressing fit with the second pressing end surface 121 of the cannula seat 11; at this time, the docking hole is communicated with the artery 21, and the connection tube 122 is communicated with the consumable pipeline, so that the artery 21 of the isolated liver 20 is communicated with the consumable pipeline.

When the connector tube assembly 10 is connected with the portal vein of the isolated liver 20, the trunk at the tail end of the portal vein is cut into the sheets 22 arranged around the periphery of the tail end of the artery 21, the portal vein is inserted into the through hole 111, the sheets 22 at the tail end of the portal vein are flatly laid on the first pressing end face 112, and the first pressing end face 112 of the butt joint seat 12 is in pressing fit with the second pressing end face 121 of the cannula seat 11. At this time, the docking hole is communicated with the portal, and in addition, the connecting tube 122 is communicated with the consumable pipeline, so that the portal of the isolated liver 20 is communicated with the consumable pipeline.

The above-mentioned connection method of the connector tube assembly 10 is not to insert the cannula directly into the artery 21 as the conventional way, so that the inner wall of the artery 21 is not contacted during the perfusion process, thereby avoiding the damage to the inner wall cells of the artery 21, and meanwhile, the suture operation is not needed, thereby greatly improving the working efficiency of the assembly, in addition, the firmness of the connection of the connector tube assembly 10 and the artery 21 or portal vein of the isolated liver 20 is higher, and the falling risk can not occur during the perfusion process.

The above-described connector tube assembly 10 may be used for both closed-loop and open-loop perfusion, and the following description will be specifically provided by way of example for open-loop perfusion.

Referring to fig. 12, fig. 12 is a schematic structural diagram of an ex vivo liver 20 perfusion system according to an embodiment of the present invention. In one embodiment, an extracorporeal liver 20 perfusion system comprises two connector tube assemblies 10 according to any of the above embodiments, and further comprises a liver reservoir 30 and a consumable line. The bottom of the liver storage device 30 is used for installing perfusate, and the liver storage device 30 is also used for placing the isolated liver 20. One of the connector tube assemblies 10 is used for communicating with the artery 21 of the isolated liver 20, the other connector tube assembly 10 is used for communicating with the portal vein of the isolated liver 20, and the connecting tube 122 is communicated with the consumable pipeline.

The perfusion system for the isolated liver 20 comprises the joint tube assembly 10, so that the technical effect of the perfusion system is brought by the joint tube assembly 10, and the beneficial effect of the perfusion system is the same as that of the joint tube assembly 10, which is not described herein again.

Furthermore, it will be appreciated that there are two consumable lines, one for each of the arteries 21 and portal vein of the isolated liver 20. One of the consumable pipelines, the artery 21 of the isolated liver 20 and the liver storage device 30 form a perfusion circulation loop, and perfusion fluid flows out of the consumable pipeline through the liver storage device 30, circularly flows into the artery 21 of the isolated liver 20 through the consumable pipeline, is discharged into the liver storage device 30 from the isolated liver 20, and circulates in this way. The other consumable pipeline, the portal vein of the isolated liver 20 and the liver storage device 30 form a perfusion circulation loop, and the perfusion liquid flows out of the consumable pipeline through the liver storage device 30, circularly flows into the portal vein of the isolated liver 20 through the consumable pipeline, is discharged into the liver storage device 30 from the isolated liver 20, and circulates in the way.

Referring to fig. 12, further, the consumable pipeline is provided with a power pump 40 and a membrane 50. Under the action of the power pump 40, the perfusate in the liver storage device 30 enters the membrane lung 50, passes through the membrane lung 50 and then enters the artery 21 or portal vein of the isolated liver 20.

Referring to fig. 12, in one embodiment, the membrane 50 is provided with a mixed gas port for communicating with a mixed gas source pipeline. The perfusate combines with the gas mixture in the membrane lung 50 to form an oxygenated perfusate to ensure that the perfusate flowing into the artery 21 has sufficient oxygen content. Specifically, the air-oxygen mixer 61 supplies a mixed gas source, the air-oxygen mixer 61 is respectively communicated with an oxygen tank 62 and a carbon dioxide tank 63 through pipelines, and the air-oxygen mixer 61 is communicated with a mixed gas interface. The oxygen from the oxygen tank 62 and the carbon dioxide from the carbon dioxide tube are mixed in the air-oxygen mixer 61 to obtain a mixed gas source, and the mixed gas source is sent into the membrane lung 50 through the mixed gas interface.

Referring to fig. 12, in addition, the membrane lung 50 is communicated with the heat exchange device 64 through a heat exchange tube to form a circulating heat exchange loop, and the perfusate exchanges heat with a heat exchange medium of the circulating heat exchange loop in the membrane lung 50 to keep the temperature of the perfusate suitable for the isolated organ. The heat exchanging device 64 is, for example, a constant temperature water tank, so that the temperature of the perfusion fluid is maintained at a predetermined temperature, for example, 37 ℃.

Referring to fig. 12, a microembolus filter 71 is further disposed on the consumable pipeline, and the microembolus filter 71 may be disposed on a pipeline connected to the liquid inlet end of the membrane lung 50, or a pipeline connected to the liquid outlet end of the membrane lung 50. Thus, the micro-suppository filter 71 is used for filtering various micro-suppositories in the perfusate, preventing the micro-blood vessels of the isolated organ from being embolized due to various micro-suppositories such as thrombus or air embolism, effectively improving the blood perfusion of the micro-blood vessels of the human body, and further improving the utilization rate of the donor organ and the success rate of the transplantation operation.

Referring to fig. 12, a pressure sensor 72 is further disposed on the consumable pipeline. The pressure sensor 72 is disposed at the fluid outlet end of the membranous lung 50 and is located close to the portal or artery 21 to measure the pressure in the portal or artery 21. Specifically, referring to fig. 1-3, a side hole luer 125 is provided on the docking station 12. A lateral bore luer fitting 125 is located on the sidewall of the connecting tube 122 and communicates with the connecting tube 122. Therefore, the pressure sensor 72 is directly connected to the lateral hole luer connector 125 through a pressure pipe, the position of the pressure connector is arranged on the docking seat 12, and the distance between the pressure connector and the position of the portal vein or artery 21 is very close, so that the pressure of the portal vein or artery 21 can be measured more truly, and the situation that the pressure pipe is connected from the middle of a circulating pipeline through the connector and is far away from the portal vein or artery 21, pressure difference is generated, and large measurement errors are caused is avoided.

Referring to fig. 12, a flow sensor 73 is further disposed on the consumable pipeline. The flow sensor 73 is disposed on the pipeline between the power pump 40 and the micro-suppository filter 71 or at the outlet end of the membrane lung 50, and the specific location is not limited herein. The flow sensor 73 can acquire the flow of the perfusion fluid on the consumable pipeline, and can judge whether the power pump 40, the micro-suppository filter 71 and the membrane lung 50 work normally or not according to the flow.

Referring to fig. 12, further, the perfusion system for the isolated liver 20 further includes a host 74, a display 75 and a power supply 76. The power supply 76 is electrically connected with the host 74, the display screen 75, the power pump 40 and the heat exchange device 64, the host 74 is electrically connected with the display screen 75, the heat exchange device 64, the pressure sensor 72 and the flow sensor 73, the host 74 can correspondingly control the display screen 75, the heat exchange device 64, the pressure sensor 72 and the flow sensor 73 to work, and the display screen 75 is used for displaying the detection data of the pressure sensor 72 and the flow sensor 73.

Referring to fig. 4, fig. 4 is a schematic structural view illustrating a cover 31 of a liver storage device 30 according to an embodiment of the present invention when closed. In one embodiment, the liver storage 30 is further provided with an openable cover 31. The junction block assembly 10 is located inside the liver reservoir 30. In this way, when the isolated liver 20 needs to be taken and placed, the cover 31 is opened. In the process of filling the isolated liver 20 into the liver storage device 30, the cover 31 is closed, and the liver storage device 30 is sealed, so as to protect and preserve moisture. The cover 31 is detachably connected to the mouth of the liver storage 30 by a plurality of fasteners 311, for example.

Referring to fig. 5 to 7, fig. 5 illustrates a schematic structural diagram of one of the viewing angles after the cover 31 of the liver storage device 30 according to an embodiment of the present invention is removed, fig. 6 illustrates a schematic structural diagram of another viewing angle after the cover 31 of the liver storage device 30 according to an embodiment of the present invention is removed, and fig. 7 illustrates a schematic structural diagram of another viewing angle after the cover 31 of the liver storage device 30 according to an embodiment of the present invention is removed. Further, the liver storage 30 includes a bottom plate 32 and a side plate 33 connected to the bottom plate 32. The side plate 33 is circumferentially arranged around the bottom plate 32, the side plate 33 is a stepped plate, and the opening size of the side plate 33 is reduced from the top to the bottom. Thus, the top opening of the side plate 33 is larger than the bottom opening of the side plate 33, and the side plate 33 is stepped, so that the top of the liver storage device 30 has enough space for the connection joint, the joint pipe assembly 10 and the related pipeline.

Referring to fig. 5-7, in one embodiment, the liver reservoir 30 includes a bottom plate 32. The bottom plate 32 is a concave plate. Further, a liquid guide groove 321 is arranged at the bottom of the concave panel, a liquid outlet 322 is arranged on the bottom wall of the liquid guide groove 321, the bottom wall of the liquid guide groove 321 is an inclined wall which is obliquely arranged, the liquid outlet 322 is arranged at the lower part of the inclined wall, and a liquid outlet joint 323 is arranged at the liquid outlet 322. Specifically, the number of the liquid outlets 322 is two, the number of the liquid outlet joints 323 is two, the two liquid outlet joints 323 are correspondingly disposed on the two liquid outlets 322 one by one, one of the liquid outlet joints 323 guides the perfusion fluid into the consumable pipeline corresponding to the artery 21, and the other liquid outlet joint 323 guides the perfusion fluid into the consumable pipeline corresponding to the portal.

Therefore, all the perfusion liquid can participate in the blood circulation in the perfusion process, and no effusion is in the container in each circulation. The design of the liquid guide groove 321 can effectively guide the discharge of the perfusion liquid in the perfusion process, avoid the formation of thrombus by the accumulated liquid, and the liquid outlet joint 323 is arranged at the lowest point of the low liquid guide groove 321, so that the perfusion liquid can rapidly flow out of the liver storage device 30 to carry out the next blood circulation. The matched liver perfusion mode is a liver open-loop type perfusion mode, that is, the perfusion liquid passing through the liver is directly discharged into the liver storage device 30, and the liquid is led out from the liquid outlet 322 of the liver storage device 30 to perform the next perfusion cycle. In order to avoid the occurrence of idle pumping during the perfusion process (the idle pumping condition is that the flow of the liquid flowing out of the in-vitro liver 20 at the same time is smaller than the flow flowing out of the liver storage device 30), the perfusate needs to store a certain amount of stable perfusate at the bottom of the liver storage device 30 to cover the liquid outlet 322 for a long time, so that the perfusate flowing out of the liver storage device and the liver can be timely replenished when the flow of the perfusate flowing out of the liver storage device is poor.

Specifically, the concave surface of the concave plate is a semi-ellipsoidal surface or a semi-hemispherical surface. The concave plate is not limited to the semi-elliptic spherical surface or the semi-spherical surface in the present embodiment, and may have other shapes, which are not limited herein. Thus, the design scheme of the bottom of the semi-elliptic spherical surface or the semi-spherical surface is that the horizontal cross-sectional area on the vertical height is gradually reduced from top to bottom, so that the volume of the lowest part is minimum, and compared with the design of equal cross-sectional area on the vertical height, the perfusion fluid is saved. That is, the corner parts of the liver storage device 30 can be prevented from having more effusion, the liver storage device 30 with larger bottom area can be prevented from consuming more perfusion fluid in the perfusion process, the perfusion fluid in the liver storage device 30 can be gathered and concentrated in the liquid guide groove 321, and the perfusion fluid is discharged to the consumable pipeline through the liquid outlet joint 323. In addition, when the concave surface of the concave plate is a semi-elliptic spherical surface or a semi-spherical surface, the perfusion liquid can be better converged and concentrated in the bottom of the liquid guide groove 321.

Furthermore, a reinforcing rib plate 324 is arranged on the bottom surface of the bottom plate 32, the reinforcing rib plate 324 is divided into a reinforcing rib plate 324 arranged transversely and a reinforcing rib plate 324 arranged longitudinally, and the reinforcing rib plate 324 is equivalent to a base of the liver storage device.

Generally, in the perfusion process, the in-vitro liver 20 is supported by the bottom of the liver storage device 30, the supporting surface is in surface contact with the in-vitro liver 20, and the surface of the liver in contact is pressed, so that unsmooth blood circulation inside the liver in the perfusion process is easily caused, and thrombus is caused.

Referring to fig. 8 to 10, fig. 8 illustrates a structural diagram of placing a balloon 81 inside fig. 7, fig. 9 illustrates a structural diagram of one view angle of placing an isolated liver 20 above the balloon 81 of fig. 8, and fig. 10 illustrates a structural diagram of another view angle of placing an isolated liver 20 above the balloon 81 of fig. 8. Further, the bottom of the liver storage 30 is provided with an air bag 81. So, through adding gasbag 81 in the bottom of storing up liver ware 30, place isolation liver 20 on gasbag 81, simulate breathing one of human lung during the perfusion, extract air and breathe in for gasbag 81 lasts the relaxation in the perfusion process, and the point of isolation liver 20 and gasbag 81 contact surface also is changing, can let isolation liver 20 more smooth and easy at the perfusion in-process blood circulation like this, avoids the isolation liver 20 surface because of receiving fixed pressure for a long time and produces the thrombus.

Referring to fig. 8, further, the airbag 81 is, for example, an elliptical ring shape, and the size of the outermost elliptical curve matches the size of the cross-sectional elliptical curve of the concave plate, so that the airbag can be placed in the concave plate, and the airbag 81 can be fixed in the concave plate with good effect. The middle part of the air bag 81 is provided with a pore, and perfusion fluid discharged outwards from the isolated liver 20 can pass through the pore and flow to the liquid guide groove 321 at the bottom of the liver storage device 30.

Referring to fig. 9-11, fig. 11 is a schematic diagram of a moisture retention membrane 82 placed over the isolated liver 20 of fig. 10. Further, the ex vivo liver 20 perfusion system also includes a moisture retention membrane 82. The moisture retention membrane 82 is used to be disposed over the excised liver 20. In this way, the isolated liver 20 is covered above the isolated liver 20 during the mechanical perfusion process, preventing the loss of water from the portal vein, artery 21 and liver surface during the perfusion process. In addition, the moisturizing membrane 82 for example with store up the back-off structure 35 that sets up on the liver ware 30 and carry out fixed cooperation, fix on storing up the liver ware 30 to can make the liver 20 of separation have certain spacing on vertical face, avoid the liver 20 of separation to upwards remove.

Referring to fig. 9 to 11, a plurality of plate penetrating connectors (341, 342, 343, 344, 345) are further disposed on the side plate 33. The plate-through joints (341, 342, 343, 344, 345) can realize the corresponding connection of the connecting pipe 122, the side hole luer joint 125 and the air bag 81 in the liver storage device 30 and the external structure. Specifically, two of the plate-through connectors 341 are cannula connectors, and the connection tube 122 is correspondingly connected to the cannula connectors through hoses, and the cannula connectors are connected to the consumable tube through tubes (e.g., hoses). Two of the two bulkhead fittings 342 are pressure tube fittings, and the lateral port luer fitting 125 is connected to a pressure tube fitting via tubing (e.g., a hose), and the pressure tube fitting is connected to a pressure sensor via tubing (e.g., a hose), and the pressure sensor senses the pressure level at the lateral port luer fitting 125 accordingly. Wherein, two are worn board joint 343 and are the trachea joint, and the air inlet of gasbag 81 and gas outlet are connected with two trachea joints respectively and correspond, and two trachea joints link to each other with external gas transmission equipment. In addition, one of the penetrating plate joints 344 is a bile pipe joint, the bile duct of the isolated liver 20 is connected to the bile pipe joint through a pipeline (e.g., a flexible pipe), and the bile duct of the isolated liver 20 discharges the bile to the outside through the flexible pipe and the bile pipe joint to the bile accommodating device 77 (shown in fig. 12). The redundant bulkhead connector 345 is a spare connector.

Referring to fig. 5 to 7 and fig. 11, the side plate 33 further includes a front side plate 331, a rear side plate 332, a left side plate 333 and a right side plate 334. The front plate 331 and the rear plate 332 are disposed opposite to each other, the left plate 333 and the right plate 334 are disposed opposite to each other, and the front plate 331, the rear plate 332, the left plate 333 and the right plate 334 surround the bottom plate 32. The front side plate 331 has two stepped shapes, an upper stepped cross section being wider than a lower stepped cross section. The upper and lower stepped vertical surfaces of the front side plate 331 are provided with joint hole sites in which the through plate joints are installed. Two joint hole sites are also provided on the left side plate 333.

Referring to fig. 5 to 7 and fig. 11, further, for example, four of the inverted structures 35 are provided, two of which are disposed on the bottom panel of the upper step of the front side plate 331, and the other two of which are disposed on the horizontal bottom panel of the upper edge surface of the elliptic curved surface of the liver storage device 30, and are disposed near the rear side plate 332. Therefore, one side of the moisturizing membrane 82 is connected with the two inverted buckle structures 35 on the bottom panel of the ladder on the front side plate 331, the other side of the moisturizing membrane 82 is connected with the two inverted buckle structures 35 on the horizontal bottom panel of the upper edge surface of the oval curved surface of the liver storage device 30, the fixing position of one side of the moisturizing membrane 82 is higher than the fixing position of the other side of the moisturizing membrane 82, and the better fixing effect on the isolated liver 20 can be realized.

The technical features of the above embodiments can be arbitrarily combined, and for the sake of brevity, all possible combinations of the technical features in the above embodiments are not described, but should be considered as the scope of the present specification as long as there is no contradiction between the combinations of the technical features.

The above-mentioned embodiments only represent some embodiments of the present invention, and the description thereof is specific and detailed, but not to be construed as limiting the scope of the present invention. It should be noted that, for those skilled in the art, without departing from the spirit of the present invention, several variations and modifications can be made, which are within the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the appended claims.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", and the like, indicate the orientation or positional relationship based on the orientation or positional relationship shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In the description of the present invention, "a plurality" means at least two, e.g., two, three, etc., unless specifically limited otherwise.

In the present invention, unless otherwise expressly stated or limited, the terms "mounted," "connected," and "fixed" are to be construed broadly and may, for example, be fixedly connected, detachably connected, or integrally formed; can be mechanically or electrically connected; they may be directly connected or indirectly connected through intervening media, or they may be connected internally or in any other suitable relationship, unless expressly stated otherwise. The specific meaning of the above terms in the present invention can be understood according to specific situations by those skilled in the art.

In the present application, unless expressly stated or limited otherwise, the first feature may be directly on or directly under the second feature or indirectly via intermediate members. Also, a first feature "on," "over," and "above" a second feature may be directly or diagonally above the second feature, or may simply indicate that the first feature is at a higher level than the second feature. A first feature being "under," "below," and "beneath" a second feature may be directly under or obliquely under the first feature, or may simply mean that the first feature is at a lesser elevation than the second feature.

It will be understood that when an element is referred to as being "secured to" or "disposed on" another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like as used herein are for illustrative purposes only and do not denote a unique embodiment.

Claims

1. A junction block assembly, comprising:

2. The connector tube assembly of claim 1, wherein one of said cannula hub sides is removably connected to one of said docking station sides and the other of said cannula hub sides is removably connected to the other of said docking station sides by a mounting member.

3. The connector tube assembly of claim 2, wherein said socket is spaced apart on one side by two U-shaped webs, said docking station is spaced apart on one side by a connecting rod and a pivot shaft connected to said connecting rod, said pivot shaft having one end rotatably disposed in and movable along one of said U-shaped webs and another end rotatably disposed in and movable along the other of said U-shaped webs, said connecting rod being movably disposed in the space between said two U-shaped webs; and a screw hole is formed in the other side part of the socket, a mounting hole corresponding to the screw hole is formed in the other side part of the butt joint seat, and the mounting part is a screw corresponding to the screw hole.

4. The connector tube assembly of claim 1, wherein the first compression end surface is planar and the second compression end surface is planar.

5. The connector assembly of claim 1, further comprising a cannula adapter disposed between the connection tube and the consumable pipeline, the cannula adapter configured to be mounted on a wall of a liver reservoir, the connection tube being connected to the consumable pipeline via the cannula adapter.

6. The junction block assembly of any one of claims 1 to 5, wherein a lateral port luer is provided on the docking station, the lateral port luer being located on a sidewall of the connecting tube and communicating with the connecting tube.

7. An ex vivo liver perfusion system, comprising two connector tube assemblies according to any one of claims 1 to 6, and further comprising a liver reservoir and consumable tubing; the bottom of the liver storage device is used for installing perfusate, the liver storage device is also used for placing the isolated liver, one of the connector tube assemblies is used for being communicated with the artery of the isolated liver, the other connector tube assembly is used for being communicated with the portal vein of the isolated liver, and the connecting tube is communicated with the consumable pipeline.

8. The extracorporeal liver perfusion system of claim 7, wherein the liver reservoir is further provided with an openable lid, the junction tube assembly being located inside the liver reservoir; store up liver ware include the bottom plate and with the curb plate that the bottom plate links to each other, the curb plate winds bottom plate circumference sets up, the curb plate is the ladder board, the opening size of curb plate is diminished by top to bottom.

9. The perfusion system for isolated liver of claim 8, wherein the liver storage device comprises a bottom plate, the bottom plate is a concave plate, a liquid guiding groove is formed in the bottom part of the concave plate, a liquid outlet is formed in the bottom wall of the liquid guiding groove, the bottom wall of the liquid guiding groove is an inclined wall which is obliquely arranged, the liquid outlet is formed in the lower part of the inclined wall, and a liquid outlet joint is formed at the liquid outlet.

10. The ex vivo liver perfusion system of claim 9, wherein the concave surface of the concave panel is a semi-ellipsoidal or semi-hemispherical surface.