CN111869657B - Isolated liver perfusion system, connector tube assembly and connection method thereof - Google Patents

Abstract

The invention relates to an isolated liver perfusion system, a joint pipe assembly and a connecting method thereof. The socket is equipped with first terminal surface that compresses tightly, and the through-hole is located the middle part of first terminal surface that compresses tightly. The butt joint seat is provided with a butt joint hole, and the butt joint hole is correspondingly arranged with the through hole. The butt joint seat is also provided with a second compaction end face. The butt joint hole is located the middle part of second compresses tightly the terminal surface, and second compresses tightly terminal surface and first and compresses tightly terminal surface compression fit. The butt joint seat is also provided with a connecting pipe communicated with the butt joint hole, and the connecting pipe is used for being communicated with a consumable pipeline. Therefore, the inner wall of the portal vein or the intrinsic artery of the liver is not contacted in the perfusion process, the endothelial tissue injury to the portal vein or the intrinsic artery of the liver is avoided, the suture operation is not needed, the assembly working efficiency is greatly improved, in addition, the joint pipe assembly is higher in firmness degree of combination with the artery or the portal vein of the isolated liver, and the falling risk can not occur in the perfusion process.

Description

Isolated liver perfusion system, joint pipe assembly and connecting method thereof

Technical Field

The invention relates to the technical field of perfusion, in particular to an isolated liver perfusion system, a joint pipe assembly and a connecting method thereof.

Background

Liver transplantation is the only effective means for treating end-stage liver disease, and because of organ shortage, many patients with liver disease end-stage and the like die without proper liver source transplantation, marginal liver supply transplantation has great significance in the liver transplantation field. The quality of the marginal liver is poor, and the damage of the liver is further aggravated by the traditional static cold preservation, and the isolated liver mechanical perfusion (Machine Perfusion, MP) technology is proposed to effectively improve the quality of the marginal liver, thereby increasing the number of transplantable livers.

In-vitro liver mechanical perfusion is a way of preserving and transferring organs, after the liver is obtained, the liver is connected with a self blood vessel to an in-vitro liver mechanical perfusion system, and the in-vitro liver mechanical perfusion system continuously perfuses perfusion fluid into the liver in the liver preserving and transferring stage and simultaneously supplies oxygen, nutrients and the like to the liver. The liver storage is the core part of the isolated liver mechanical perfusion system, and when perfusion is carried out, the liver is placed in the liver storage and is connected into the circulating pipeline through the cannula and the connector on the liver storage. The perfusion mode of the liver in the mechanical perfusion system can be divided into two modes according to the pipeline connection mode, namely ① closed-loop perfusion, namely, a consumable pipeline is connected with a hepatic artery and a portal vein of the liver through a cannula to serve as a perfusate inlet of the liver, and is connected with a inferior vena cava of the liver to serve as a perfusate outlet of the liver through the cannula to form a closed circulation pipeline. ② The infusion liquid directly flows out of the liver storage from the inferior vena cava after flowing through the liver, and is discharged into the consumable pipeline from a liquid outlet of the liver storage to form an open-loop circulation pipeline.

When in perfusion, the liver storage serves as an intermediate carrier, and the liver is connected into the circulating pipeline for circulating perfusion, so that the liver storage is designed to meet the requirements that the liver is comfortably perfused and can be perfectly connected into the circulating pipeline for perfusion. The arterial cannula or portal cannula of the liver is a medium for connecting the liver to a circulating pipeline, and is generally long tubular, one end of the cannula is connected to the pipeline during perfusion, the other end of the cannula is inserted into the portal or venous vessel of the liver, and the cannula is fastened and fixed by an operation wire for perfusion.

Disclosure of Invention

Based on this, it is necessary to overcome the defects of the prior art, and to provide an isolated liver perfusion system, a joint tube assembly and a connection method thereof, which can facilitate assembly and connection and can avoid damage to the endothelial tissue of the portal vein of the isolated liver or the intrinsic artery of the liver.

The technical scheme is that the joint pipe assembly comprises:

the cannula seat is provided with a through hole for inserting an artery or a portal of the isolated liver, the cannula seat is provided with a first compression end face, and the through hole is positioned in the middle of the first compression end face;

the butt joint seat, the butt joint seat is equipped with the butt joint hole, the butt joint hole with the corresponding setting in through-hole position, the butt joint seat still is equipped with the second and compresses tightly the terminal surface, the butt joint hole is located the second compresses tightly the middle part of terminal surface, the second compress tightly the terminal surface with first compress tightly terminal surface compression fit, the butt joint seat still be equipped with the connecting pipe that the butt joint hole is linked together, the connecting pipe is used for being linked together with the consumptive material pipeline.

The joint pipe assembly can connect the artery or portal of the isolated liver with the consumable pipeline, so that the artery or portal of the isolated liver is connected into the perfusion circulation pipeline. The connection process of the artery and the joint pipe assembly is described by connecting the artery of the isolated liver with the consumable pipe as an example, the trunk of the tail end of the artery is reserved before perfusion, the trunk of the tail end of the artery is sheared into sheets circumferentially arranged around the tail end of the artery, the artery is inserted into the through hole, the sheets of the tail end of the artery are tiled on the first compression end face, the first compression end face of the butt joint seat is in compression fit with the second compression end face of the socket, at the moment, the butt joint hole is communicated with the artery, and in addition, the connecting pipe is communicated with the consumable pipe, so that the artery of the isolated liver is communicated with the consumable pipe. After the isolated liver is connected into the perfusion circulation pipeline, the perfusion work can be started, after the perfusion is finished, the butt joint seat and the insertion tube seat are separated, and the sheet material at the tail end of the artery is trimmed, so that the perfusion is finished. Therefore, the cannula is not directly inserted into the artery as in the conventional method, so that the inner wall of the arterial vessel is not contacted in the perfusion process, the damage to the endothelial tissue of the artery is avoided, the suture operation is not needed, the assembly working efficiency is greatly improved, in addition, the joint tube assembly is higher in firmness degree of combination with the artery or the portal of the isolated liver, and the falling risk is avoided in the perfusion process.

In one embodiment, one side of the socket is movably connected with one side of the counter-seat, and the other side of the socket is detachably connected with the other side of the counter-seat through a mounting piece.

In one embodiment, two U-shaped connecting plates are arranged at intervals on one side of the cannula seat, a connecting rod and a rotating shaft connected with the connecting rod are arranged on one side of the docking seat, one end of the rotating shaft is rotatably arranged in one U-shaped connecting plate and can move along the other U-shaped connecting plate, the other end of the rotating shaft is rotatably arranged in the other U-shaped connecting plate and can move along the other U-shaped connecting plate, the connecting rod is movably located at an interval between the two U-shaped connecting plates, a screw hole is formed in the other side of the cannula seat, a mounting hole corresponding to the screw hole is formed in the other side of the docking seat, and the mounting piece is a screw rod corresponding to the screw hole.

In one embodiment, the first compression end face is planar and the second compression end face is planar.

In one embodiment, the connector tube assembly further comprises a cannula connector disposed between the connecting tube and the consumable tube, the cannula connector is configured to be mounted on a wall of the liver storage device, and the connecting tube is connected to the consumable tube through the cannula connector.

In one embodiment, the docking station is provided with a side hole luer connector which is positioned on the side wall of the connecting pipe and communicated with the connecting pipe.

A method of connecting the joint pipe assembly, comprising the steps of:

When the joint pipe assembly is connected with an artery of an isolated liver, cutting a trunk at the tail end of the artery into sheets circumferentially arranged around the tail end of the artery, inserting the artery into the through hole, spreading the sheets at the tail end of the artery on the first compression end surface, and performing compression fit on the first compression end surface of the butt joint seat and the second compression end surface of the insertion seat;

When the joint pipe assembly is connected with the portal vein of the isolated liver, the trunk at the tail end of the portal vein is cut into sheets circumferentially arranged around the tail end of the artery, the portal vein is inserted into the through hole, the sheets at the tail end of the portal vein are flatly paved on the first compression end face, and the first compression end face of the butt joint seat is in compression fit with the second compression end face of the insertion tube seat.

The connecting method of the joint pipe assembly is not like the traditional method that the cannula is directly inserted into the artery, so that the inner wall of the artery is not contacted in the pouring process, the damage to the endothelial tissue of the artery is avoided, the suture operation is not needed, the assembling working efficiency is greatly improved, in addition, the joint pipe assembly is higher in firmness degree of combination with the artery or the portal of the isolated liver, and the falling risk is avoided in the pouring process.

The isolated liver perfusion system comprises two joint pipe assemblies, a liver storage and a consumable pipeline, wherein the bottom of the liver storage is used for installing perfusate, the liver storage is also used for placing isolated livers, one joint pipe assembly is used for being communicated with arteries of the isolated livers, the other joint pipe assembly is used for being communicated with portal veins of the isolated livers, and the connecting pipe is communicated with the consumable pipeline.

The technical effect of the isolated liver perfusion system is brought by the joint pipe assembly due to the fact that the isolated liver perfusion system comprises the joint pipe assembly, and the beneficial effect of the isolated liver perfusion system is the same as that of the joint pipe assembly, and the isolated liver perfusion system is not described in detail herein.

In one embodiment, the liver storage device is further provided with an openable cover body, the joint pipe assembly is located in the liver storage device, the liver storage device comprises a bottom plate and side plates connected with the bottom plate, the side plates are circumferentially arranged around the bottom plate, the side plates are stepped plates, and the opening size of the side plates is reduced from top to bottom.

In one embodiment, the liver storage device comprises a bottom plate, the bottom plate is a concave plate, a liquid guide groove is formed in the bottom portion of the concave plate, a liquid outlet is formed in the bottom wall of the liquid guide groove, the bottom wall of the liquid guide groove is an inclined wall which is obliquely arranged, the liquid outlet is arranged at the lower portion of the inclined wall, a liquid outlet connector is arranged at the liquid outlet, and the concave surface of the concave plate is a semi-elliptic spherical surface or a hemispherical surface.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this specification, illustrate embodiments of the application and together with the description serve to explain the application.

In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings required for the description of the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments of the present invention, and other drawings may be obtained according to these drawings without inventive effort for a person skilled in the art.

FIG. 1 is a schematic view of a joint tube assembly according to an embodiment of the present invention combined with an ex vivo liver;

FIG. 2 is a schematic view of a joint pipe assembly according to an embodiment of the present invention;

FIG. 3 is a schematic view of a joint pipe assembly according to an embodiment of the present invention;

Fig. 4 is a schematic view of a structure of a cover of a liver accumulator according to an embodiment of the present invention when the cover is closed;

FIG. 5 is a schematic view of a liver accumulator according to an embodiment of the present invention with a cover removed from the liver accumulator;

FIG. 6 is a schematic view of a liver accumulator according to an embodiment of the present invention from another perspective with a cover removed;

FIG. 7 is a schematic view of a liver accumulator according to an embodiment of the present invention from a further perspective with a cover removed;

FIG. 8 is a schematic view of the structure of the air bag placed inside of FIG. 7;

FIG. 9 is a schematic view of one view of the isolated liver positioned over the balloon of FIG. 8;

FIG. 10 is another perspective schematic view of the placement of an isolated liver over the balloon of FIG. 8;

FIG. 11 is a schematic view of the placement of a moisturizing film over the isolated liver of FIG. 10;

fig. 12 is a schematic structural diagram of an isolated liver perfusion system according to an embodiment of the present invention.

10. The connector tube assembly comprises a connector tube assembly body, 11, a cannula seat, 111, a through hole, 112, a first compression end face, 113, a screw hole, 114, a U-shaped connecting plate, 1141, an anti-falling flange, 12, a docking seat, 121, a second compression end face, 122, a connecting tube, 123, a mounting hole, 124, a mounting piece, 125, a side hole luer connector, 126, a connecting rod, 127, a rotating shaft, 20, an isolated liver, 21, an artery, 22, a sheet, 30, a liver storage, 31, a cover body, 311, a fastener, 32, a bottom plate, 321, a liquid guide groove, 322, a liquid outlet, 323, a liquid outlet connector, 324, a reinforcing rib plate, 33, a side plate, 331, a front side plate, 332, a rear side plate, 333, a left side plate, 334, a right side plate, 341, 342, 343, 344, 345, a penetrating plate connector, 35, a back-fastening structure, 40, a power pump, 50, a membrane lung, 61, an air-oxygen mixer, 62, an oxygen tank, 63, a carbon dioxide tank, 64, a heat exchange device, 71, a micro-bolt filter, 72, a pressure sensor, 73, a flow sensor, 74, a host, 75, a display screen, a 75, a power source, a humidity-preserving device, a power source, a membrane, a 81 and a water-preserving device.

Detailed Description

In order that the above objects, features and advantages of the invention will be readily understood, a more particular description of the invention will be rendered by reference to the appended drawings. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present invention. The present invention may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the invention, whereby the invention is not limited to the specific embodiments disclosed below.

Generally, the cannulas corresponding to the conventional liver artery and the cannulas corresponding to the portal vein are generally in a tube shape, after the cannulas are inserted into the artery of the liver or the portal vein of the liver, medical staff still need to tighten and fix the cannulas by using surgical wires, the operation is complicated, the tightening of the surgical wires is unstable, the risk of the cannulas falling off exists in the perfusion process, and the portal vein or the arterial vascular endothelial tissue can be damaged in the process of inserting the cannulas into the artery or the portal vein of the liver.

Referring now to fig. 1 to 3, fig. 1 is a schematic view showing a structure of a joint tube assembly 10 according to an embodiment of the present invention combined with an isolated liver 20, fig. 2 is a schematic view showing a structure of a joint tube assembly 10 according to an embodiment of the present invention, and fig. 3 is a schematic view showing a structure of a joint tube assembly 10 according to an embodiment of the present invention. In one embodiment of the present invention, a joint pipe assembly 10 is provided, wherein the joint pipe assembly 10 includes a cannula holder 11 and a docking holder 12.

The cannula holder 11 is provided with a through hole 111 for inserting an artery 21 or portal of the isolated liver 20, the cannula holder 11 is provided with a first compression end surface 112, and the through hole 111 is located in the middle of the first compression end surface 112. The docking base 12 is provided with a docking hole, and the docking hole is arranged corresponding to the position of the through hole 111. The docking station 12 is further provided with a second compression end face 121. The butt joint hole is positioned in the middle of the second pressing end surface 121, and the second pressing end surface 121 is in pressing fit with the first pressing end surface 112. The docking station 12 is further provided with a connection pipe 122 communicated with the docking hole, and the connection pipe 122 is used for being communicated with a consumable pipeline.

The above-mentioned connector tube assembly 10 can connect the artery 21 or portal of the isolated liver 20 with the consumable pipeline, so that the artery 21 or portal of the isolated liver 20 is connected into the perfusion circulation pipeline. Taking the connection of the artery 21 and the connector tube assembly 10 as an example, the connection process of the artery 21 and the consumable tube assembly 10 is described, the trunk at the tail end of the artery 21 is reserved before perfusion, the trunk at the tail end of the artery 21 is cut into sheets 22 circumferentially arranged around the tail end of the artery 21, the artery 21 is inserted into the through hole 111, the sheets 22 at the tail end of the artery 21 are flatly paved on the first compression end face 112, the first compression end face 112 of the butt joint seat 12 is in compression fit with the second compression end face 121 of the cannula seat 11, at the moment, the butt joint hole is communicated with the artery 21, and in addition, the connecting tube 122 is communicated with the consumable tube assembly, so that the artery 21 of the isolated liver 20 is communicated with the consumable tube assembly. After the isolated liver 20 is connected into the perfusion circulation pipeline, the perfusion work can be started, after the perfusion is finished, the butt joint seat 12 and the cannula seat 11 are separated, and the sheet 22 at the tail end of the artery 21 is trimmed off, so that the perfusion is finished. Thus, the cannula is not directly inserted into the artery 21 as conventionally, so that the inner wall of the artery 21 is not contacted in the process of perfusion, damage to the endothelial tissue of the artery 21 is avoided, suture operation is not needed, the assembly working efficiency is greatly improved, and in addition, the joint tube assembly 10 is higher in firmness degree of combination with the artery 21 or the portal of the isolated liver 20, and the risk of falling off in the process of perfusion is avoided.

Referring to fig. 1 to 3, further, one side of the cannula holder 11 is movably connected to one side of the docking holder 12, and the other side of the cannula holder 11 is detachably connected to the other side of the docking holder 12 through a mounting member 124. In this way, since one side of the socket 11 is movably connected with one side of the docking station 12, when the socket 11 and the docking station 12 are opened and closed, the other side of the socket 11 and the other side of the docking station 12 are assembled together by the mounting member 124, so that the opening and closing operations between the socket 11 and the docking station 12 can be conveniently performed.

It should be noted that "movable" in the "movable" connection in this embodiment is relative to "fixed", and when two components are "movable" in connection, it should be understood that the two components are not "fixed" in connection, but are, for example, rotationally connected, and/or movable in either direction, etc.

Specifically, one of the sides of the docking station 12 is not only movable relative to one of the sides of the cannula holder 11 in a direction perpendicular to the first pressing end surface 112, but also the one of the sides of the docking station 12 is rotatable relative to one of the sides of the cannula holder 11. In this way, when the docking station 12 and the cannula station 11 are in the open state, since one side of the docking station 12 can move in the direction perpendicular to the first compression end surface 112 relative to one side of the cannula station 11, the first compression end surface 112 and the second compression end surface 121 are in relatively separated positions, so that the first compression end surface 112 and the second compression end surface 121 can better fix the sheet 22 at the end of the artery 21.

In a specific embodiment, referring to fig. 3, two U-shaped connection plates 114 are disposed at a side of the socket 11 at intervals, a connection rod 126 and a rotation shaft 127 connected to the connection rod 126 are disposed at a side of the docking station 12, one end of the rotation shaft 127 is rotatably disposed in one of the U-shaped connection plates 114 and can move along one of the U-shaped connection plates 114, and the other end of the rotation shaft 127 is rotatably disposed in the other U-shaped connection plate 114 and can move along the other U-shaped connection plate 114, and the connection rod 126 is movably disposed at an interval between the two U-shaped connection plates 114.

Further, the mouth portion of the U-shaped connection plate 114 is provided with an anti-drop flange 1141. The anti-falling flange 1141 is used for limiting and abutting against the rotation shaft 127, so as to prevent the rotation shaft 127 from moving outwards from the mouth of the U-shaped connection plate 114. The U-shaped connection plate 114 is made of elastic material, so that under the condition that the acting force is applied to the rotation shaft 127, the rotation shaft 127 can move outwards from the opening of the U-shaped connection plate 114, namely the socket 11 is separated from the docking seat 12, and the treatment such as cleaning and maintenance can be performed. The reverse procedure also facilitates assembly of cannula hub 11 with docking hub 12.

It will be appreciated that, in order to enable one side of the docking station 12 to move relative to one side of the socket 11 in a direction perpendicular to the first pressing end surface 112, the one side of the socket 11 may also rotate relative to one side of the socket 11, which is not limited to the above-mentioned U-shaped connection plate 114, connection rod 126 and rotation shaft 127, and the manner in which one side of the socket 11 is engaged with one side of the docking station 12 may also be other designs, which are not limited herein. For example, one side of the socket holder 11 is provided with a guide rail, one side of the docking station 12 is provided with a slider that cooperates with the guide rail, and the slider is slidably disposed on the guide rail, thereby enabling the docking station 12 to move relative to the socket holder 11. For another example, the rotation shaft 127 is rotatably connected to the connecting rod 126, so that the docking station 12 can be rotatably closed on the cannula station 11.

Referring to fig. 1 to 3, further, a screw hole 113 is formed on the other side portion of the socket 11, a mounting hole 123 corresponding to the screw hole 113 is formed on the other side portion of the docking station 12, and the mounting member 124 is a screw corresponding to the screw hole 113.

In an alternative embodiment, instead of a combination of the cannula holder 11 and the docking holder 12, in which one side is rotatably connected and the other side is detachably connected, the cannula holder 11 as a whole is detachably connected to the docking holder 12, specifically, the connection manner of the cannula holder 11 and the docking holder 12 may be, for example, a snap connection, a rivet connection, a screw connection, a bolt connection, a pin connection, or the like, which is not limited herein.

Referring to fig. 1-3, in one embodiment, the first compression end 112 is planar and the second compression end 121 is planar. In this way, the first compression end surface 112 and the second compression end surface 121 can firmly compress the sheet 22 at the tail end of the artery 21 when in compression fit, so as to ensure tightness. Alternatively, the first pressing end surface 112 may also be an arcuate surface, and the second pressing end surface 121 is an arcuate surface corresponding to the first pressing end surface 112. The first compression end surface 112 may be a surface having another shape, and the second compression end surface 121 may be a surface that is adapted to the shape of the first compression end surface 112, so long as the sheet 22 that firmly compresses the distal end of the artery 21 when the first compression end surface 112 and the second compression end surface 121 are in compression fit can be realized.

Referring to fig. 1 to 3, in one embodiment, the connector tube assembly 10 further includes a cannula connector (specifically, two bulkhead connectors 341 in fig. 4) disposed between the connecting tube 122 and the consumable pipeline. The cannula fitting is adapted to be mounted on the wall of the liver reservoir 30, and the connecting tube 122 is connected to the consumable tubing through the cannula fitting. In this way, it is possible to facilitate connection of the connection tube 122 inside the liver reservoir 30 to the consumable line outside the liver reservoir 30.

Referring to fig. 1-3, in one embodiment, a side port luer fitting 125 is provided on the docking station 12. A side port luer fitting 125 is located on the side wall of the connection tube 122 and communicates with the connection tube 122. Thus, the pressure tube can be directly connected to the side hole luer connector 125, the pressure connector is arranged on the butt seat 12, the position distance between the pressure connector and the portal or artery 21 is very close, the pressure of the portal or artery 21 can be measured more truly, the situation that the pressure tube is connected by installing the connector in the middle of the circulating pipeline, the pressure tube is far away from the portal or artery 21, the pressure difference is generated, and the measurement error is large is avoided.

In one embodiment, a method of connecting the joint pipe assembly 10 of any of the above embodiments includes the steps of:

When the joint tube assembly 10 is connected with the artery 21 of the isolated liver 20, the trunk at the tail end of the artery 21 is cut into sheets 22 circumferentially arranged around the tail end of the artery 21, the artery 21 is inserted into the through hole 111, the sheets 22 at the tail end of the artery 21 are flatly laid on the first compression end face 112, the first compression end face 112 of the butt joint seat 12 is in compression fit with the second compression end face 121 of the intubation seat 11, at the moment, the butt joint hole is communicated with the artery 21, and in addition, the connecting tube 122 is communicated with a consumable pipeline, so that the artery 21 of the isolated liver 20 is communicated with the consumable pipeline.

When the connector tube assembly 10 is connected with the portal vein of the isolated liver 20, the trunk at the tail end of the portal vein is cut into sheets 22 circumferentially arranged around the tail end of the artery 21, the portal vein is inserted into the through hole 111, the sheets 22 at the tail end of the portal vein are flatly laid on the first compression end face 112, and the first compression end face 112 of the butt joint seat 12 is in compression fit with the second compression end face 121 of the cannula seat 11. At this time, the butt joint hole is communicated with the portal, and in addition, the connecting pipe 122 is communicated with the consumable pipeline, so that the portal of the isolated liver 20 is communicated with the consumable pipeline.

The above-mentioned connecting method of the joint tube assembly 10 is not as conventional that the cannula is directly inserted into the artery 21, so that the inner wall of the artery 21 is not contacted during the perfusion process, the damage to the cells on the inner wall of the artery 21 is avoided, and the suture operation is not needed, so that the working efficiency of the assembly is greatly improved, and in addition, the joint tube assembly 10 has higher firmness degree in combination with the artery 21 or the portal of the isolated liver 20, and the falling risk does not occur during the perfusion process.

The above-described connector tube assembly 10 may be used for both closed-type pouring and open-loop type pouring, and will be described below by way of example.

Referring to fig. 12, fig. 12 is a schematic diagram illustrating an isolated liver 20 perfusion system according to an embodiment of the present invention. In one embodiment, an ex vivo liver 20 perfusion system includes two connector tube assemblies 10 of any of the above embodiments, and further includes a liver reservoir 30 and consumable tubing. The bottom of the liver reservoir 30 is used for installing perfusate, and the liver reservoir 30 is also used for placing the isolated liver 20. One of the connector tube assemblies 10 is used for communicating with the artery 21 of the isolated liver 20, the other connector tube assembly 10 is used for communicating with the portal vein of the isolated liver 20, and the connecting tube 122 is communicated with the consumable pipeline.

The above-mentioned isolated liver 20 perfusion system, because of including the above-mentioned joint pipe assembly 10, its technical effect has the joint pipe assembly 10 to bring, and beneficial effect is the same with the beneficial effect of joint pipe assembly 10, and the description is omitted here.

In addition, it can be understood that the number of consumable pipelines is two, and the two consumable pipelines are arranged in one-to-one correspondence with the artery 21 and the portal vein of the isolated liver 20. One of the consumable pipelines, the artery 21 of the isolated liver 20 and the liver storage 30 form a perfusion circulation loop, and perfusate flows out of the consumable pipeline through the liver storage 30 and flows into the artery 21 of the isolated liver 20 in a circulation mode through the consumable pipeline, and is discharged into the liver storage 30 from the isolated liver 20, and the perfusion circulation loop circulates in this mode. The other consumable pipeline, the portal vein of the isolated liver 20 and the liver storage 30 form a perfusion circulation loop, and perfusate flows out of the consumable pipeline through the liver storage 30 and flows into the portal vein of the isolated liver 20 in a circulating way through the consumable pipeline, and is discharged into the liver storage 30 from the isolated liver 20, so that the perfusion circulation loop circulates.

Referring to fig. 12, further, a power pump 40 and a membrane lung 50 are disposed on the consumable pipeline. Under the action of the power pump 40, the perfusate of the liver reservoir 30 enters the membrane lung 50, passes through the membrane lung 50, and then enters the artery 21 or portal of the isolated liver 20.

Referring to fig. 12, in one embodiment, a membrane lung 50 is provided with a gas mixture port for communicating with a gas mixture line. The perfusate combines with the gas mixture in the membrane lung 50 to form an oxygenated perfusate to ensure that the oxygen content of the perfusate flowing into the artery 21 is sufficient. Specifically, the air-fuel mixture is supplied from an air-fuel mixture 61, the air-fuel mixture 61 is respectively communicated with an oxygen tank 62 and a carbon dioxide tank 63 through pipelines, and the air-fuel mixture 61 is communicated with an air-fuel mixture interface. The oxygen from the oxygen tank 62 and the carbon dioxide from the carbon dioxide tube are mixed in the air-oxygen mixer 61 to obtain a mixed gas source, and the mixed gas source is sent into the membrane lung 50 through a mixed gas interface.

Referring to fig. 12, in addition, the membrane lung 50 is communicated with the heat exchange device 64 through a heat exchange tube to form a circulating heat exchange loop, and the perfusate exchanges heat with a heat exchange medium of the circulating heat exchange loop in the membrane lung 50 to keep the temperature of the perfusate suitable for the isolated organ. The heat exchange device 64 is in particular, for example, a thermostatic water tank, so that the temperature of the perfusate is maintained at a preset temperature, for example 37 ℃.

Referring to fig. 12, further, a micro-plug filter 71 is further disposed on the consumable pipeline, and the micro-plug filter 71 may be disposed on a pipeline connected to the liquid inlet end of the membrane lung 50 or a pipeline connected to the liquid outlet end of the membrane lung 50. Thus, the micro-plug filter 71 is used for filtering various micro-plugs in the perfusate, preventing the micro-vessels of the isolated organs from being plugged due to various micro-plugs such as thrombus or air plug, effectively improving the blood perfusion of the micro-vessels of the human body, and further improving the utilization rate of the donor organs and the success rate of the transplantation operation.

Referring to fig. 12, further, a pressure sensor 72 is further disposed on the consumable pipeline. The pressure sensor 72 is disposed at the outflow end of the membrane lung 50 and is located close to the portal vein or artery 21 to measure the pressure of the portal vein or artery 21. Specifically, referring to fig. 1 to 3, the docking station 12 is provided with a side port luer 125. A side port luer fitting 125 is located on the side wall of the connection tube 122 and communicates with the connection tube 122. In this way, the pressure sensor 72 is directly connected to the side port luer connector 125 through the pressure pipe, the pressure connector is arranged on the butt seat 12, and the position distance between the pressure connector and the portal or artery 21 is very close, so that the pressure of the portal or artery 21 can be measured more truly, the situation that the pressure pipe is connected by installing the connector in the middle of the circulating pipeline, the pressure pipe is far away from the portal or artery 21, a pressure difference is generated, and a larger measuring error is caused is avoided.

Referring to fig. 12, further, a flow sensor 73 is further disposed on the consumable pipeline. The flow sensor 73 is provided on the line between the power pump 40 and the micro-plug filter 71 or on the liquid outlet end of the membrane lung 50, and the specific position is not limited herein. The flow sensor 73 can obtain the flow of the perfusate on the consumable pipeline, and can judge whether the power pump 40, the micro-plug filter 71 and the membrane lung 50 work normally or not according to the flow.

Referring to fig. 12, the infusion system for the isolated liver 20 further includes a host 74, a display 75 and a power source 76. The power supply 76 is electrically connected with the host computer 74, the display screen 75, the power pump 40 and the heat exchange device 64, the host computer 74 is electrically connected with the display screen 75, the heat exchange device 64, the pressure sensor 72 and the flow sensor 73, the host computer 74 can correspondingly control the display screen 75, the heat exchange device 64, the pressure sensor 72 and the flow sensor 73 to work, and the display screen 75 is used for displaying detection data of the pressure sensor 72 and the flow sensor 73.

Referring to fig. 4, fig. 4 is a schematic diagram illustrating a structure of the liver container 30 when the cover 31 is closed according to an embodiment of the present invention. In one embodiment, the liver reservoir 30 is further provided with an openable cover 31. The connector tube assembly 10 is located inside the liver reservoir 30. Thus, when it is necessary to take and place the isolated liver 20, the lid 31 is opened. In the process of filling the isolated liver 20 into the liver storage 30, the cover 31 is closed, and the liver storage 30 is sealed, so that the effects of protection and moisture preservation are achieved. The cover 31 is detachably connected to the mouth of the liver container 30 by a plurality of fasteners 311, for example.

Referring to fig. 5 to 7, fig. 5 is a schematic structural view of a liver accumulator 30 according to an embodiment of the present invention from one view after the cover 31 is removed, fig. 6 is a schematic structural view of a liver accumulator 30 according to an embodiment of the present invention from another view after the cover 31 is removed, and fig. 7 is a schematic structural view of a liver accumulator 30 according to an embodiment of the present invention from another view after the cover 31 is removed. Further, the liver container 30 includes a bottom plate 32 and a side plate 33 connected to the bottom plate 32. The side plates 33 are circumferentially arranged around the bottom plate 32, the side plates 33 are stepped plates, and the opening size of the side plates 33 becomes smaller from top to bottom. Thus, the top opening of the side plate 33 is larger than the bottom opening of the side plate 33, and the side plate 33 is stepped so that the top position of the liver container 30 has sufficient space to accommodate the connection fitting, the fitting tube assembly 10 and the associated tubing.

Referring to fig. 5-7, in one embodiment, the liver reservoir 30 includes a floor 32. The bottom plate 32 is a concave plate. Further, a liquid guiding groove 321 is arranged at the bottom part of the concave panel, a liquid outlet 322 is arranged on the bottom wall of the liquid guiding groove 321, the bottom wall of the liquid guiding groove 321 is an inclined wall which is obliquely arranged, the liquid outlet 322 is arranged at the lower part of the inclined wall, and a liquid outlet connector 323 is arranged at the liquid outlet 322. Specifically, the number of the liquid outlets 322 is two, the number of the liquid outlet connectors 323 is two, the two liquid outlet connectors 323 are arranged at the two liquid outlet outlets 322 in a one-to-one correspondence manner, one liquid outlet connector 323 guides the perfusate into the consumable pipeline corresponding to the artery 21, and the other liquid outlet connector 323 guides the perfusate into the consumable pipeline corresponding to the portal.

Thus, all perfusate can participate in blood circulation in the process of perfusion conveniently, and any effusion is not in the container in each circulation. The liquid guide groove 321 is designed to more effectively guide the discharge of perfusion liquid in the perfusion process, so as to avoid thrombus formation by accumulated liquid, and the liquid outlet connector 323 is arranged at the lowest point of the low liquid guide groove 321, so that the perfusion liquid can rapidly flow out of the liver storage 30 for the next blood circulation. Because the matched liver perfusion mode is an open-loop liver perfusion mode, namely, the perfusion liquid passing through the liver is directly discharged into the liver storage 30, and the liquid is led out from the liquid outlet 322 of the liver storage 30 for the next round of perfusion circulation. In order to avoid empty pumping (the flow of the liquid flowing out of the isolated liver 20 is smaller than the flow flowing out of the liver storage 30 in the same time) during the perfusion process, the perfusate needs to be stored in a certain amount at the bottom of the liver storage 30, and the perfusate with stable amount covers the liquid outlet 322 for a long time, so that the perfusate can be timely supplied when the liver storage container and the liver flowing out of the perfusate are possibly bad.

Specifically, the concave surface of the concave panel is a semi-ellipsoidal or hemispherical surface. The concave panel is not limited to the semi-elliptical spherical surface or the semi-spherical surface in the present embodiment, but may be other shapes, and is not limited thereto. In this way, the bottom design of the semi-elliptic or hemispherical surface gradually reduces the horizontal cross-sectional area from top to bottom in the vertical height, so that the volume of the lowest part is the smallest, and compared with the design of the equal cross-sectional area in the vertical height, the perfusion liquid is saved. That is, the liquid accumulation at the corners of the liver accumulator 30 can be avoided, the liquid accumulation at the corners of the liver accumulator 30 with larger bottom area can be avoided, the liquid accumulation in the liver accumulator 30 can be concentrated into the liquid guiding groove 321, and the liquid can be discharged to the consumable pipeline through the liquid outlet connector 323. In addition, when the concave surface of the concave panel is a semi-elliptic spherical surface or a semi-spherical surface, the perfusate can be better concentrated in the bottom of the liquid guide groove 321.

Further, the bottom surface of the bottom plate 32 is provided with a reinforcing rib plate 324, and the reinforcing rib plate 324 is divided into a reinforcing rib plate 324 arranged transversely and a reinforcing rib plate 324 arranged longitudinally, and the reinforcing rib plate 324 is equivalent to a base of the liver accumulator.

Generally, during the perfusion process, the isolated liver 20 is supported by the bottom of the liver storage 30, and the support surface contacts with the isolated liver 20 surface, so that the contact part of the liver surface is stressed, and the blood circulation inside the liver is easy to be unsmooth during the perfusion process, thereby causing thrombus.

Referring to fig. 8 to 10, fig. 8 illustrates a schematic structure of the balloon 81 placed inside of fig. 7, fig. 9 illustrates a schematic structure of one view of the isolated liver 20 placed above the balloon 81 of fig. 8, and fig. 10 illustrates another view of the isolated liver 20 placed above the balloon 81 of fig. 8. Further, the bottom of the liver accumulator 30 is provided with an air bag 81. In this way, by adding the air bag 81 at the bottom of the liver storage 30, the isolated liver 20 is placed on the air bag 81, and one-breathing of the human lung is simulated during perfusion, and the air bag 81 is pumped and sucked, so that the air bag 81 continuously relaxes in the perfusion process, and the contact point of the isolated liver 20 and the air bag 81 also changes, thus the blood circulation of the isolated liver 20 in the perfusion process is smoother, and the thrombus generated on the surface of the isolated liver 20 due to long-time fixed pressure is avoided.

Referring to fig. 8, further, the air bag 81 is, for example, in an elliptical ring shape, and the outermost elliptical curve thereof matches the elliptical curve of the concave plate, so that the air bag 81 can be just placed in the concave plate, and the fixing effect of the air bag 81 in the concave plate is good. The central portion of the balloon 81 has a hole through which the perfusate discharged from the isolated liver 20 can pass and flow into the sump 321 at the bottom of the liver reservoir 30.

Referring to fig. 9-11, fig. 11 illustrates a schematic structure of a moisturizing film 82 placed over the isolated liver 20 of fig. 10. Further, the ex vivo liver 20 perfusion system also includes a moisturizing film 82. The moisturizing film 82 is for placement over the isolated liver 20. In this way, the isolated liver 20 is covered over the isolated liver 20 during mechanical infusion, preventing loss of portal, arterial 21 and liver surface moisture during infusion. In addition, the moisturizing film 82 is fixedly matched with the back-off structure 35 arranged on the liver storage 30, and is fixed on the liver storage 30, so that the isolated liver 20 has a certain limit on the vertical surface, and the isolated liver 20 is prevented from moving upwards.

Referring to fig. 9 to 11, further, the side plate 33 is provided with a plurality of through-plate connectors (341, 342, 343, 344, 345). The threading connectors (341, 342, 343, 344, 345) can correspondingly connect the connecting pipe 122, the side hole luer connector 125 and the air bag 81 inside the liver storage 30 with the external structure. Specifically, two of the threading connectors 341 are cannula connectors, and the connecting tube 122 is correspondingly connected with the cannula connectors through hoses, and the cannula connectors are connected with consumable pipelines through pipelines (such as hoses). The two through-board connectors 342 are pressure pipe connectors, the side hole luer connectors 125 are connected with the pressure pipe connectors through pipelines (such as hoses), the pressure pipe connectors are connected with pressure sensors through pipelines (such as hoses), and the pressure sensors correspondingly sense the pressure at the side hole luer connectors 125. Wherein two board penetrating connectors 343 are air pipe connectors, the air inlet and the air outlet of air bag 81 are respectively connected with two air pipe connectors correspondingly, and the two air pipe connectors are connected with external air transmission equipment. In addition, one of the plate connectors 344 is a bile duct connector, and the bile duct of the isolated liver 20 is connected to the bile duct connector through a pipeline (e.g., a hose), and the bile duct of the isolated liver 20 discharges bile to the outside through the hose and the bile duct connector into the bile receiving apparatus 77 (shown in fig. 12). The redundant bulkhead connector 345 is a spare connector.

Referring to fig. 5 to 7 and 11, the side plates 33 further include a front side plate 331, a rear side plate 332, a left side plate 333 and a right side plate 334. The front plate 331 is disposed opposite to the rear plate 332, the left plate 333 is disposed opposite to the right plate 334, and the front plate 331, the rear plate 332, the left plate 333, and the right plate 334 are respectively surrounded on the periphery of the bottom plate 32. The front plate 331 has a two-step shape, and the upper step cross section is wider than the lower step cross section. The vertical surfaces of the upper and lower steps of the front side plate 331 are provided with joint holes, and the threading joint is arranged in the joint holes. Two joint holes are also provided on the left side plate 333.

Referring to fig. 5 to 7 and 11, further, the number of the back-off structures 35 is four, for example, two of them are disposed on the bottom plate of the step on the front plate 331, and the other two are disposed on the horizontal bottom plate of the upper surface of the elliptic curved surface of the liver container 30, and are disposed near the rear plate 332. In this way, one side of the moisturizing film 82 is connected with the two back-off structures 35 on the bottom panel of the step on the front side plate 331, the other side of the moisturizing film 82 is connected with the two back-off structures 35 on the horizontal bottom panel of the upper edge surface of the elliptic curved surface of the liver storage 30, and the fixing position of one side of the moisturizing film 82 is higher than that of the other side of the moisturizing film 82 by Yu Bao, so that a better fixing effect on the isolated liver 20 can be realized.

The technical features of the above embodiments may be arbitrarily combined, and all possible combinations of the technical features in the above embodiments are not described for brevity of description, however, as long as there is no contradiction between the combinations of the technical features, they should be considered as the scope of the description.

The above examples illustrate only a few embodiments of the invention, which are described in detail and are not to be construed as limiting the scope of the invention. It should be noted that it will be apparent to those skilled in the art that several variations and modifications can be made without departing from the spirit of the invention, which are all within the scope of the invention. Accordingly, the scope of protection of the present invention is to be determined by the appended claims.

In the description of the present invention, it should be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", "axial", "radial", "circumferential", etc. indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings are merely for convenience in describing the present invention and simplifying the description, and do not indicate or imply that the device or element being referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first," "second," and the like, are used for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature. In the description of the present invention, the meaning of "plurality" means at least two, for example, two, three, etc., unless specifically defined otherwise.

In the present invention, unless explicitly specified and limited otherwise, the terms "mounted," "connected," "secured," and the like are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally formed, mechanically connected, electrically connected, directly connected, indirectly connected through an intervening medium, or in communication between two elements or in an interaction relationship between two elements, unless otherwise explicitly specified. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art according to the specific circumstances.

In the present invention, unless expressly stated or limited otherwise, a first feature "up" or "down" a second feature may be the first and second features in direct contact, or the first and second features in indirect contact via an intervening medium. Moreover, a first feature being "above," "over" and "on" a second feature may be a first feature being directly above or obliquely above the second feature, or simply indicating that the first feature is level higher than the second feature. The first feature being "under", "below" and "beneath" the second feature may be the first feature being directly under or obliquely below the second feature, or simply indicating that the first feature is less level than the second feature.

It will be understood that when an element is referred to as being "fixed" or "disposed" on another element, it can be directly on the other element or intervening elements may also be present. When an element is referred to as being "connected" to another element, it can be directly connected to the other element or intervening elements may also be present. The terms "vertical," "horizontal," "upper," "lower," "left," "right," and the like are used herein for illustrative purposes only and are not meant to be the only embodiment.

Claims (10)

1. A fitting tube assembly, the fitting tube assembly comprising:

the cannula seat is provided with a through hole for inserting an artery or a portal of the isolated liver, the cannula seat is provided with a first compression end face, and the through hole is positioned in the middle of the first compression end face;

The butt joint seat is provided with a butt joint hole, the butt joint hole is correspondingly arranged at the position of the through hole, the butt joint seat is also provided with a second compression end face, the butt joint hole is positioned in the middle of the second compression end face, the second compression end face is in compression fit with the first compression end face, the butt joint seat is also provided with a connecting pipe communicated with the butt joint hole and used for being communicated with a consumable pipeline, one side of the pipe inserting seat is movably connected with one side of the butt joint seat, the other side of the pipe inserting seat is detachably connected with the other side of the butt joint seat through a mounting piece, two U-shaped connecting plates are arranged on one side of the pipe inserting seat at intervals, a connecting rod and a rotating shaft connected with the connecting rod are arranged on one side of the butt joint seat, one end of the rotating shaft is rotatably arranged in one U-shaped connecting plate and can move along the other U-shaped connecting plate, the other end of the rotating shaft is rotatably arranged in the other U-shaped connecting plate and can move along the other U-shaped connecting plate, the connecting rod is movably positioned between the two U-shaped connecting plates and is movably connected with the other U-shaped connecting plate, a screw hole is correspondingly arranged on the side of the other side of the pipe inserting seat, and the screw bolt is correspondingly arranged on the side of the butt joint seat.

2. The connector tube assembly of claim 1, wherein the mouth portion of the U-shaped connecting plate is provided with an anti-drop flange for limited interference fit with the rotating shaft.

3. The connector tube assembly of claim 2, wherein the U-shaped connector plate is made of an elastic material.

4. The fitting tube assembly of claim 1 wherein said first compression end surface is planar and said second compression end surface is planar.

5. The connector tube assembly of claim 1, further comprising a cannula connector disposed between the connecting tube and the consumable tube, the cannula connector for mounting to a wall of a liver reservoir, the connecting tube being connected to the consumable tube by the cannula connector.

6. A connector tube assembly according to any one of claims 1 to 5, wherein the docking station is provided with a side port luer connector located on a side wall of the connection tube and in communication with the connection tube.

7. A method of connecting a fitting tube assembly as claimed in any one of claims 1 to 6, comprising the steps of:

When the joint pipe assembly is connected with an artery of an isolated liver, cutting a trunk at the tail end of the artery into sheets circumferentially arranged around the tail end of the artery, inserting the artery into the through hole, spreading the sheets at the tail end of the artery on the first compression end surface, and performing compression fit on the first compression end surface of the butt joint seat and the second compression end surface of the insertion seat;

When the joint pipe assembly is connected with the portal vein of the isolated liver, the trunk at the tail end of the portal vein is cut into sheets circumferentially arranged around the tail end of the artery, the portal vein is inserted into the through hole, the sheets at the tail end of the portal vein are flatly paved on the first compression end face, and the first compression end face of the butt joint seat is in compression fit with the second compression end face of the insertion tube seat.

8. An isolated liver perfusion system is characterized by comprising two joint pipe assemblies according to any one of claims 1 to 6, a liver storage device and a consumable pipeline, wherein the bottom of the liver storage device is used for containing perfusate, the liver storage device is also used for placing isolated livers, one joint pipe assembly is used for being communicated with arteries of the isolated livers, the other joint pipe assembly is used for being communicated with portal vessels of the isolated livers, and the connecting pipe is communicated with the consumable pipeline.

9. The isolated liver perfusion system of claim 8, wherein the liver accumulator is further provided with an openable cover, the connector tube assembly is located inside the liver accumulator, the liver accumulator comprises a bottom plate and a side plate connected with the bottom plate, the side plate is circumferentially arranged around the bottom plate, the side plate is a stepped plate, and the opening size of the side plate is reduced from top to bottom.

10. The isolated liver perfusion system of claim 8, wherein the liver storage comprises a bottom plate, the bottom plate is a concave plate, a liquid guide groove is arranged at the bottom part of the concave plate, a liquid outlet is arranged on the bottom wall of the liquid guide groove, the bottom wall of the liquid guide groove is an inclined wall which is obliquely arranged, the liquid outlet is arranged at the lower part of the inclined wall, a liquid outlet joint is arranged at the liquid outlet, and the concave surface of the concave plate is a semi-elliptic spherical surface or a hemispherical surface.