CN211426363U - Nucleic acid electrophoresis cell with heating function - Google Patents
Nucleic acid electrophoresis cell with heating function Download PDFInfo
- Publication number
- CN211426363U CN211426363U CN201922435365.8U CN201922435365U CN211426363U CN 211426363 U CN211426363 U CN 211426363U CN 201922435365 U CN201922435365 U CN 201922435365U CN 211426363 U CN211426363 U CN 211426363U
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- Prior art keywords
- electrophoresis
- electrophoresis cell
- cell body
- power supply
- nucleic acid
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- 238000001962 electrophoresis Methods 0.000 title claims abstract description 76
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 18
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 18
- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 18
- 238000010438 heat treatment Methods 0.000 title claims abstract description 17
- 210000005056 cell body Anatomy 0.000 claims abstract description 30
- 210000004027 cell Anatomy 0.000 claims abstract description 16
- 238000005485 electric heating Methods 0.000 claims abstract description 16
- 238000004026 adhesive bonding Methods 0.000 claims abstract description 3
- 108020004414 DNA Proteins 0.000 abstract description 5
- 238000000034 method Methods 0.000 abstract description 5
- 238000009434 installation Methods 0.000 abstract description 4
- 102000053602 DNA Human genes 0.000 abstract description 3
- 230000015556 catabolic process Effects 0.000 abstract description 3
- 238000006731 degradation reaction Methods 0.000 abstract description 3
- NOIIUHRQUVNIDD-UHFFFAOYSA-N 3-[[oxo(pyridin-4-yl)methyl]hydrazo]-N-(phenylmethyl)propanamide Chemical compound C=1C=CC=CC=1CNC(=O)CCNNC(=O)C1=CC=NC=C1 NOIIUHRQUVNIDD-UHFFFAOYSA-N 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 239000003292 glue Substances 0.000 description 3
- 230000009182 swimming Effects 0.000 description 2
- 229920000936 Agarose Polymers 0.000 description 1
- 239000002390 adhesive tape Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000005684 electric field Effects 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 229920002521 macromolecule Polymers 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a nucleic acid electrophoresis technical field especially relates to a nucleic acid electrophoresis cell with heating function, including the electrophoresis cell body, the electrophoresis cell body is by interior inner wall, constant temperature electric heating layer and outer wall of having set gradually outside to, the both sides of electrophoresis cell body up end are fixed with curb plate and fixed box through screw horizontal installation, positive power has been placed in the fixed box of one side, negative power has been placed in the fixed box of opposite side, the through-hole has all been seted up to the both sides on electrophoresis cell body upper portion, the through-hole internal gluing is fixed with the fixed block, the up end horizontal cover of electrophoresis cell body is equipped with the apron, the both sides of apron all are fixed with power plug through the mounting screw, and power plug passes through the electric wire and is connected with DC power supply. The utility model discloses in, gel in the help electrophoresis tank that can be fine keeps in the required temperature of fine work, reduces the DNA molecule and takes place the degradation because of the temperature reason in carrying out the electrophoresis operation process to better improvement the accuracy of electrophoresis operation result.
Description
Technical Field
The utility model relates to a nucleic acid electrophoresis technical field especially relates to a nucleic acid electrophoresis cell with heating function.
Background
Electrophoresis refers to a phenomenon that charged particles or molecules move in an electric field, a technology for separating substances by utilizing the electrophoresis phenomenon is called an electrophoresis technology, most of biological macromolecules such as nucleic acid and the like can be separated by the electrophoresis technology, the electrophoresis technology is an indispensable important analysis means for molecular biology research, the conventional electrophoresis device has single function, gel cannot always keep good temperature required by work, and DNA molecules are easily degraded in the electrophoresis operation process, so that the accuracy of an electrophoresis result is influenced.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the defects existing in the prior art and providing a nucleic acid electrophoresis cell with a heating function.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
the utility model provides a nucleic acid electrophoresis cell with heating function, including the electrophoresis cell body, the electrophoresis cell body is by interior inner wall, constant temperature electric heating layer and outer wall of having set gradually outside to, the both sides of electrophoresis cell body up end are fixed with the curb plate through screw horizontal installation, the through-hole has been seted up to one side of curb plate, and insert perpendicularly in the through-hole and establish and be fixed with fixed box, positive power has been placed in the fixed box of one side, negative power has been placed in the fixed box of opposite side, the through-hole has all been seted up to the both sides on electrophoresis cell body upper portion, it is fixed with the fixed block to glue in the through-hole, and the through-hole has been seted up at the middle part of fixed block, the up end horizontal cover of electrophoresis cell body is equipped with the apron, the both sides of.
Preferably, the constant-temperature electric heating layer is located between the inner wall and the outer wall, and the constant-temperature electric heating layer is connected with the outside through an electric wire.
Preferably, the output end of the positive power supply is connected with a positive power supply lead, and the positive power supply lead penetrates through a through hole in the middle of the fixing block and is communicated with the inside of the electrophoresis cell body.
Preferably, the output end of the negative power supply is connected with a negative power supply lead, and the negative power supply lead penetrates through a through hole in the middle of the fixing block and is communicated with the inside of the electrophoresis cell body.
Preferably, the cover plate is horizontally provided with an electrophoresis comb groove.
The utility model has the advantages that: the utility model discloses in, keep away inside through the pond that has the nucleic acid electrophoresis cell of heating function here and set up logical groove to at logical inslot installation constant temperature electric heating layer, when carrying out the electrophoresis operation to molecular biology research, gel in the help electrophoresis tank that constant temperature electric heating layer can be fine keeps in the required temperature of fine work, reduces the DNA molecule and takes place the degradation because of the temperature reason in carrying out the electrophoresis operation process, thereby the better accuracy that has improved the electrophoresis operation result.
Drawings
FIG. 1 is a schematic view of a nucleic acid electrophoresis cell with heating function according to the present invention;
FIG. 2 is a schematic top view of the electrophoresis cell body of the nucleic acid electrophoresis cell with heating function according to the present invention;
FIG. 3 is a schematic top view of the cover plate of the nucleic acid electrophoresis cell with heating function according to the present invention.
In the figure: the swimming pool comprises an electrophoresis pool body 1, an inner wall 2, a constant-temperature electric heating layer 3, an outer wall 4, a side plate 5, a fixing box 6, a positive power supply 71, a negative power supply 72, a cover plate 8, a power plug 9, a positive power supply lead 101, a negative power supply lead 102 and a fixing block 11.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
Referring to fig. 1-3, a nucleic acid electrophoresis cell with heating function, including electrophoresis cell body 1, said electrophoresis cell body 1 has inner wall 2, constant temperature electric heating layer 3 and outer wall 4 from inside to outside in proper order, constant temperature electric heating layer 3 locates between inner wall 2 and outer wall 4, and constant temperature electric heating layer 3 connects with external battery through the electric wire, when carrying out electrophoresis operation to the molecular biology research, the gel that constant temperature electric heating layer 3 can be fine help in electrophoresis cell body 1 keeps in the fine required temperature of work, reduce DNA molecule and take place to degrade because of the temperature reason in carrying out the electrophoresis operation process, thus the accuracy of the electrophoresis operation result is better improved, both sides of the upper end face of electrophoresis cell body 1 are fixed with curb plate 5 through the horizontal installation of screw, one side of curb plate 5 has been seted up the through-hole, and fixed box 6 is inserted and fixed vertically in the through-hole, a positive power supply 71 is arranged in the fixing box 6 at one side, the output end of the positive power supply 71 is connected with a positive power supply lead 101, and the positive power lead 101 passes through the through hole in the middle of the fixing block 11 and is communicated with the inside of the swimming pool body 1, the negative power 72 is arranged in the fixing box 6 at the other side, the output end of the negative power 72 is connected with the negative power lead 102, and the cathode power lead 102 passes through the through hole in the middle of the fixing block 11 and is communicated with the inside of the electrophoresis cell body 1, both sides of the upper part of the electrophoresis cell body 1 are provided with through holes, the fixing block 11 is fixed in the through holes by gluing, and the through-hole has been seted up at the middle part of fixed block 11, and the up end level of electrophoresis cell body 1 is covered and is equipped with apron 8, and the electrophoresis comb groove has been seted up to apron 8 upper level, is convenient for follow-up electrophoresis operation that carries on, and the both sides of apron 8 all are fixed with power plug 9 through the mounting screw, and power plug 9 passes through the electric wire and is connected with DC power supply.
In the embodiment, when the nucleic acid electrophoresis cell with heating function is used, an operator firstly takes the gel making groove in the electrophoresis cell body 1 to clean and dry, then puts the gel making glass plate into the gel making glass plate, takes the transparent adhesive tape to seal the glass plate and the edges of the two ends of the inner groove to form a mold, then puts the inner groove in a horizontal position, puts the electrophoresis comb in a fixed position, opens the power supply of the constant temperature electric heating layer 3, the constant temperature electric heating layer 3 can well help the gel in the electrophoresis cell body 1 to be kept in a good temperature required by work, reduces the degradation of DNA molecules due to temperature during the electrophoresis operation process, thereby better improving the accuracy of the electrophoresis operation result, the cover plate 8 is covered on the electrophoresis cell body 1, the power plugs 9 are inserted at the two sides of the cover plate 8, the power plugs 9 are respectively connected with the positive power supply 71 and the negative power supply 72, and after the agarose liquid is cooled to about 65 ℃, slowly spreading the glue solution until a uniform glue layer is formed on the surface of the whole glass plate, standing at room temperature until the gel is completely solidified, slightly pulling the electrophoresis comb vertically along an electrophoresis comb groove formed in the cover plate 8, moving the sample from the negative electrode to the positive electrode, turning on a power supply and carrying out subsequent electrophoresis operation.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (5)
1. A nucleic acid electrophoresis cell with a heating function comprises an electrophoresis cell body (1), and is characterized in that the electrophoresis cell body (1) is sequentially provided with an inner wall (2), a constant-temperature electric heating layer (3) and an outer wall (4) from inside to outside, two sides of the upper end surface of the electrophoresis cell body (1) are horizontally provided with side plates (5) through screws, one side of each side plate (5) is provided with a through hole, a fixing box (6) is vertically inserted and fixed in each through hole, an anode power supply (71) is arranged in the fixing box (6) at one side, a cathode power supply (72) is arranged in the fixing box (6) at the other side, two sides of the upper part of the electrophoresis cell body (1) are provided with through holes, a fixing block (11) is fixed in each through hole in a gluing manner, a through hole is formed in the middle of the fixing block (11), a cover plate (8) is horizontally covered on the upper end surface of the electrophoresis cell body (1), power plugs, and the power plug (9) is connected with a direct current power supply through a wire.
2. The nucleic acid electrophoresis cell with the heating function as claimed in claim 1, wherein the constant temperature electric heating layer (3) is located between the inner wall (2) and the outer wall (4), and the constant temperature electric heating layer (3) is connected with the outside through an electric wire.
3. The nucleic acid electrophoresis cell with the heating function as claimed in claim 1, wherein the output end of the positive power supply (71) is connected with a positive power supply lead (101), and the positive power supply lead (101) passes through a through hole in the middle of the fixing block (11) and is communicated with the inside of the electrophoresis cell body (1).
4. The nucleic acid electrophoresis cell with the heating function as claimed in claim 1, wherein the output end of the negative power supply (72) is connected with a negative power supply lead (102), and the negative power supply lead (102) passes through the through hole in the middle of the fixing block (11) and is communicated with the inside of the electrophoresis cell body (1).
5. The nucleic acid electrophoresis cell with the heating function as claimed in claim 1, wherein the cover plate (8) is horizontally provided with an electrophoresis comb groove.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922435365.8U CN211426363U (en) | 2019-12-30 | 2019-12-30 | Nucleic acid electrophoresis cell with heating function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201922435365.8U CN211426363U (en) | 2019-12-30 | 2019-12-30 | Nucleic acid electrophoresis cell with heating function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN211426363U true CN211426363U (en) | 2020-09-04 |
Family
ID=72281307
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201922435365.8U Active CN211426363U (en) | 2019-12-30 | 2019-12-30 | Nucleic acid electrophoresis cell with heating function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN211426363U (en) |
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2019
- 2019-12-30 CN CN201922435365.8U patent/CN211426363U/en active Active
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