CN201166647Y - Separation gum-making vertical cataphoresis apparatus - Google Patents
Separation gum-making vertical cataphoresis apparatus Download PDFInfo
- Publication number
- CN201166647Y CN201166647Y CNU2008200038776U CN200820003877U CN201166647Y CN 201166647 Y CN201166647 Y CN 201166647Y CN U2008200038776 U CNU2008200038776 U CN U2008200038776U CN 200820003877 U CN200820003877 U CN 200820003877U CN 201166647 Y CN201166647 Y CN 201166647Y
- Authority
- CN
- China
- Prior art keywords
- groove
- glue
- negative electrode
- last
- positive electrode
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001962 electrophoresis Methods 0.000 title claims abstract description 19
- 238000000926 separation method Methods 0.000 title claims description 10
- 239000003292 glue Substances 0.000 claims abstract description 42
- 238000007789 sealing Methods 0.000 claims description 4
- 125000003003 spiro group Chemical group 0.000 claims description 4
- 210000002421 cell wall Anatomy 0.000 claims description 3
- 238000002474 experimental method Methods 0.000 abstract description 13
- 230000000694 effects Effects 0.000 abstract description 9
- 239000011521 glass Substances 0.000 description 3
- 230000005684 electric field Effects 0.000 description 2
- 239000000499 gel Substances 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 238000013517 stratification Methods 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012467 final product Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000005012 migration Effects 0.000 description 1
- 238000013508 migration Methods 0.000 description 1
- 239000000178 monomer Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 230000009182 swimming Effects 0.000 description 1
Images
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model discloses a vertical electrophoresis apparatus for a separate glue production, which adopts a glue making groove which is orderly provided with a lower groove and an upper groove, wherein the upper end surface of the glue-making groove is provided with a glue-making groove which allows the lower end of the upper end to be arranged, the inner side of the lower groove is a positive electrode groove whose lower end is communicated with a positive electrode rod with an electrode line, the lower end of the upper groove is inserted in the positive electrode groove, the upper groove is U-shaped, both sides of the upper groove are provided with glue-arranging plates, both sides of the upper end of the upper groove and the two glue-arranging plates form a negative electrode groove whose lower end is communicated with a negative electrode rod with an electrode line, and a clamp device is arranged on the upper wall of the upper groove, which is corresponding to both sides of the glue-arranging plates. The electrophoresis apparatus adopts the separated combined type structure and has the function of making glue, and the clamping and the fixing are simple, and the utility model has the advantages of simple structure, reasonable design, convenient use, small volume, rapid experiment speed and good experiment effect.
Description
Technical field
The utility model belongs to a kind of separation glue vertical electrophoresis instrument.
Background technology
The agarose gel electrophoresis instrument be separate being used to of using always, the instrument of identification of dna, RNA molecule mixture, this electrophoresis apparatus with agar gel as holder, utilize charge effect and the molecular sieving effect of DVA molecule when swimming, reach the purpose of separating mixture.Dna molecular is electronegative in being higher than the solution of its isoelectric point, and anode moves in electric field.Under certain electric field intensity, the migration velocity of dna molecular depends on molecular sieving effect, and promptly the size of molecule itself and configuration are main influence factors.Electrophoresis apparatus is divided into level and vertical stratification, the electrophoresis apparatus of horizontal structure is put the offset plate horizontal positioned, the electrophoresis apparatus of vertical stratification is put offset plate and is vertically placed, should but both communicate by the glue groove with the glue groove and be positioned at the slot electrode at its two ends, and the positive and negative electrode line that is positioned at slot electrode is formed.Traditional vertical electrophoresis instrument is put the offset plate monomer and is set to integrative-structure, putting the offset plate body places, when two put offset plate body placement Ago-Gel experiment thing, also must on the glue platform that other establishes, carry out, then in that being put, it in electrophoresis apparatus, uses bolt, chucking fixing operation trouble, and speed of experiment is slow, structural design is unreasonable, and result of use is poor.
Summary of the invention
The purpose of this utility model is a kind of separation glue vertical electrophoresis instrument of design, adopts the separate combination type structure, and glue platform function is arranged, and chucking is fixing easy, has simple in structure, reasonable in design, easy to use, volume is little, the fast and good advantage of experiment effect of speed of experiment.For this reason, the utility model adopts glue groove upper end to fall to being provided with down groove successively, goes up groove, glue groove upper surface is provided with keeps supplying the glue groove of putting into the groove lower end, in the groove of following groove is the positive electrode groove, the communicate positive electrode bar of charged polar curve of positive electrode groove lower end, last groove lower end is plugged in the positive electrode groove, last groove takes the shape of the letter U, last groove both sides are provided with puts offset plate, both sides and two, last groove upper end are put offset plate and are surrounded the negative electrode groove, the communicate negative electrode bar of charged polar curve of negative electrode groove lower end, the last cell wall corresponding with putting the offset plate both sides is provided with clamp device.The said structure design has realized the purpose of this utility model.
The utility model has the advantages that and adopt the separate combination type structure, glue platform function is arranged, and chucking is fixing easy, have simple in structurely, reasonable in design, easy to use, volume is little, and speed of experiment is fast and experiment effect is good.
Description of drawings
Fig. 1 is the utility model split status architecture synoptic diagram
Fig. 2 is an assembled state structural representation of the present utility model
Fig. 3 is the groove structural representation of going up of the present utility model
Fig. 4 is groove and the clamp device structural representation gone up of the present utility model
Embodiment
As shown in Figures 1 to 4, a kind of separation glue vertical electrophoresis instrument, mainly by glue groove 11, down groove 14, go up groove 3 and cover 2 and form.
As depicted in figs. 1 and 2, glue groove upper end falls to being provided with down groove successively, goes up groove.Lid is fastened on the groove upper end.Glue groove upper surface is provided with keeps supplying the glue groove of putting into the groove lower end 10, and glue groove lower surface should be the plane.Described glue groove sidewall is provided with fastening button 9.Fastening button axle is located on the glue groove sidewall, fastening button outer end is the convex handle, is provided with back-moving spring 19 between handle and the corresponding glue groove sidewall, fixingly only need outwards pull out fastening button when going up groove and places and go up groove, then unclamp fastening button under action of reset spring, fastening button chucking is gone up groove.The fastening groove of going up of fastening button is beneficial to what two blocks of glass were formed and two puts and pour experimental raw in the offset plate respectively in the glue groove.Is positive electrode groove 12 in the groove of following groove, positive electrode groove lower end is by the communicate positive electrode bar 6 of charged polar curve 13 of the notch 5 that communicates with the positive electrode bar; Be negative electrode bar dependency structure also in like manner, so be not repeated for last groove.It is solid in glue groove upper end to fall for ease of following groove, establishes four projections 7 in following groove lower end, and the glue groove upper surface corresponding with projection is provided with the draw-in groove 8 that snaps in for projection.Glue groove lower end is provided with supporting leg 20 for four jiaos, is used to support the glue groove.
Described positive and negative electrode bar is spirally connected by spiro union portion 17 with corresponding groove up and down.Described electrode wires is positioned on the periphery wall of positive and negative electrode bar.Obviously, the electrode wires in each slot electrode lays respectively on the positive and negative electrode bar, and the socket aperture 24 that the other end by the positive and negative electrode bar is provided with joins with external power supply.Be provided with O-ring seal 18 between positive and negative electrode bar and the sidewall of groove up and down that penetrates.The effect of O-ring seal is a sealing positive and negative electrode bar and up and down between the groove sidewall.Turn the positive and negative electrode bar and detach up and down groove, replaceable electrode wires on it.
As shown in Figure 3, last groove lower end is plugged in the positive electrode groove, and last groove takes the shape of the letter U, and last groove both sides are provided with two and put offset plate 15, and each is put offset plate and is made of apart from the glass plate that is provided with two block gaps.Two block gaps are used to place Ago-Gel experiment thing apart from the space between the glass plate that is provided with.Two two putting offset plate and accelerated speed of experiment of putting.Both sides and two, last groove upper end are put offset plate and are surrounded negative electrode groove 16, the communicate negative electrode bar 4 of charged polar curve of negative electrode groove lower end, and the last cell wall corresponding with putting the offset plate both sides is provided with clamp device.The described groove of going up is provided with sealing strip 23, and its effect is that the interface between offset plate and the corresponding last groove is put in effectively sealing.
As shown in Figure 4, described clamp device is formed by folder button 1 with card division, top 21, spring 25 and fixing button screw rod 22 that the folder button fuses, the fixing button screw rod is fixed on the groove by spiro union portion 26, fixing button screw rod middle part axle is worn folder button middle part, is provided with spring between the stomidium in the fixing button screw rod other end and the folder button.Put offset plate on last groove the time when needs clamp, outwards to pull out earlier the folder button and change card division (shape as shown in Figure 3) outside last groove, top outward, to put into and put offset plate after on the last groove, interior again commentaries on classics withstands on card division, top card for 180 ° and puts on the offset plate, unclamp the folder button, under spring action, card division, top card withstands on puts offset plate on last groove.
During use, putting earlier two of offset plate respectively in the glue groove puts and puts into experimental raw in the offset plate respectively, can carry out two collagen material experiments of putting simultaneously, again glue groove, following groove and last groove are fallen to setting up jointly the integrator structure, in the positive and negative electrode groove, place conducting liquid, again the positive and negative electrode plug is inserted on the socket aperture respectively, and energized, adjusting voltage stabilizing output, experimentizing gets final product.
In a word, the utility model adopts the separate combination type structure, and glue platform function is arranged, and chucking is fixing easy, Have simple in structurely, reasonable in design, easy to use, volume is little, and speed of experiment is fast and experiment effect is good, can push away The wide use.
Claims (6)
1, a kind of separation glue vertical electrophoresis instrument, mainly by the glue groove, following groove, last groove and lid are formed, it is characterized in that: glue groove upper end falls to being provided with down groove successively, last groove, glue groove upper surface is provided with keeps supplying the glue groove of putting into the groove lower end, in the groove of following groove is the positive electrode groove, the communicate positive electrode bar of charged polar curve of positive electrode groove lower end, last groove lower end is plugged in the positive electrode groove, last groove takes the shape of the letter U, last groove both sides are provided with puts offset plate, both sides and two, last groove upper end are put offset plate and are surrounded the negative electrode groove, the communicate negative electrode bar of charged polar curve of negative electrode groove lower end, and the last cell wall corresponding with putting the offset plate both sides is provided with clamp device.
2, by the described separation glue of claim 1 vertical electrophoresis instrument, it is characterized in that: described glue groove sidewall is provided with fastening button.
3, by the described separation glue of claim 1 vertical electrophoresis instrument, it is characterized in that: described positive and negative electrode bar is spirally connected by spiro union portion with corresponding groove up and down.
4, by the described separation glue of claim 1 vertical electrophoresis instrument, it is characterized in that: described electrode wires is positioned on the periphery wall of positive and negative electrode bar.
5, by the described separation glue of claim 1 vertical electrophoresis instrument, it is characterized in that: the described groove of going up is provided with sealing strip.
6, by the described separation glue of claim 1 vertical electrophoresis instrument, it is characterized in that: described clamp device is formed by the folder button with card division, top, spring and fixing button screw rod that the folder button fuses, the fixing button screw rod is fixed on the groove by spiro union portion, fixing button screw rod middle part axle is worn folder button middle part, is provided with spring between the stomidium in the fixing button screw rod other end and the folder button.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200038776U CN201166647Y (en) | 2008-03-03 | 2008-03-03 | Separation gum-making vertical cataphoresis apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNU2008200038776U CN201166647Y (en) | 2008-03-03 | 2008-03-03 | Separation gum-making vertical cataphoresis apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201166647Y true CN201166647Y (en) | 2008-12-17 |
Family
ID=40192037
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNU2008200038776U Expired - Fee Related CN201166647Y (en) | 2008-03-03 | 2008-03-03 | Separation gum-making vertical cataphoresis apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201166647Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105466994A (en) * | 2015-12-30 | 2016-04-06 | 中国计量学院 | Electrophoresis tank device for isoelectric focusing electrophoresis |
| CN107831207A (en) * | 2017-12-07 | 2018-03-23 | 安徽师范大学 | A kind of glue utensil and its application method |
| CN110186988A (en) * | 2019-06-18 | 2019-08-30 | 王尧 | A kind of high throughput agarose gel electrophoresis device |
-
2008
- 2008-03-03 CN CNU2008200038776U patent/CN201166647Y/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105466994A (en) * | 2015-12-30 | 2016-04-06 | 中国计量学院 | Electrophoresis tank device for isoelectric focusing electrophoresis |
| CN105466994B (en) * | 2015-12-30 | 2018-08-03 | 中国计量学院 | A kind of electrophoresis slot device for isoelectric focusing electrophoresis |
| CN107831207A (en) * | 2017-12-07 | 2018-03-23 | 安徽师范大学 | A kind of glue utensil and its application method |
| CN107831207B (en) * | 2017-12-07 | 2024-03-26 | 安徽师范大学 | Glue making device and use method thereof |
| CN110186988A (en) * | 2019-06-18 | 2019-08-30 | 王尧 | A kind of high throughput agarose gel electrophoresis device |
| CN110186988B (en) * | 2019-06-18 | 2022-07-05 | 安阳工学院 | High-flux agarose gel electrophoresis device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20081217 Termination date: 20100303 |