CN211149095U - A multicolor laser beam combining device for flow cytometer - Google Patents

Abstract

The present application discloses a multi-color laser beam combining device for flow cytometer, comprising a multi-color laser and a beam combining prism; the beam combining prism is a dichroic mirror; the multi-color laser includes a first atom that emits light of different wavelengths The laser, the last sub-laser, and at least one median sub-laser between the first sub-laser and the last sub-laser; the direction of the emitted light of the first sub-laser, the last sub-laser and each of the median sub-lasers is the same as the direction of the emitted light. The included angle of the dichroic mirror is 45°, so as to combine the emitted light of different wavelengths. The structure design of the device has the advantages of low cost, compact structure, and little influence on the external environment temperature change.

Description

A multicolor laser beam combining device for flow cytometer

technical field

The present application relates to the technical field of laser beam combining, in particular to a multicolor laser beam combining device for flow cytometer.

Background technique

Flow cytometer is an instrument that uses laser as light source to perform multi-parameter rapid analysis and sorting of cells or other various particles in liquid flow. It can be divided into four functional systems: liquid flow system, optical system Detection system, electronic control system, data storage and analysis system. The optical system usually includes lasers with multiple wavelengths. The laser beams of different wavelengths are integrated into one beam or parallel beams with small spacing through the beam combining module, and are focused into the flow chamber through the focusing mirror.

The multi-color laser beam combining module usually uses the same number of adjustable beam combiners as the laser channels to combine lasers of different wavelengths into one laser, and each beam combiner reflects the laser of its corresponding wavelength respectively. As shown in FIG. 1 , FIG. 1 is a schematic structural diagram of a multicolor laser beam combining device in the prior art. In FIG. 1 , the polychromatic laser 1 and the beam combining prism 2 form an included angle of 45°. Multiple separate beam-combining prisms are assembled on the optical system platform, and the beam-combining prisms and the beam-combining mirror frame are fixed by mechanical installation. The pointing stability of this module is easily affected by the ambient temperature.

Utility model content

The technical problem to be solved by the present application is to provide a multi-color laser beam combining device for flow cytometer, the structure design of the device has the advantages of low cost, compact structure, and little influence on the external environment temperature change.

In order to solve the above technical problems, the present application provides a polychromatic laser beam combining device for flow cytometer, comprising a polychromatic laser and a beam combining prism; it is characterized in that, the beam combining prism is a dichroic mirror; The laser includes a first sub-laser that emits light of different wavelengths, a last sub-laser, and at least one median sub-laser between the first sub-laser and the last sub-laser; the first sub-laser, the last sub-laser and each of the middle sub-lasers; The angle between the direction of the emitted light of the sub-laser and the dichroic mirror is 45°, so as to combine the emitted light of different wavelengths.

Optionally, the dichroic mirror includes a first mirror surface close to the polychromatic laser and a second mirror surface parallel to the first mirror surface;

The first mirror surface is provided with a first anti-reflection film at the position opposite to the first photon laser, and the second mirror surface is provided with a first reflective film at the position opposite to the first anti-reflection film, so that the first photon The light emitted by the laser is transmitted through the first anti-reflection film, and then incident on the first reflective film for reflection.

Optionally, the first mirror surface is provided with a first dichroic transflective film at the position opposite to the median sub-laser, and the second mirror surface is provided with the opposite position of the first dichroic transflective film. the second reflective film, so that the light emitted by the neutral laser is transmitted through the first dichroic transflective film, and the light reflected by the first reflective film is incident on the first dichroic transflective film for reflection , the formed two beams of light are converged into one beam and incident on the second reflective film.

Optionally, a second dichroic transflective film is provided at a position opposite to the last sub-laser on the first mirror surface, and a position opposite to the second dichroic transflective film is provided on the second mirror surface. The second anti-reflection film, so that the light emitted by the last sub-laser is transmitted through the second dichroic transflective film, and the light reflected by the second reflective film is incident on the second dichroic transflective film. After reflection, the formed two beams of light are converged into one beam and incident on the second anti-reflection film.

Optionally, a pointing adjustment module is further provided between the polychromatic laser and the dichroic mirror.

Optionally, a laser shaping module is further provided before the pointing adjustment module.

In the present application, the beam combining device includes a polychromatic laser and a beam combining prism; it is characterized in that, the beam combining prism is a dichroic mirror; the polychromatic laser includes a first sub-laser, a last sub-laser and At least one neutral sub-laser between the first sub-laser and the last sub-laser; the direction of the emitted light of the first sub-laser, the last sub-laser and each of the median sub-lasers is the same as the direction of the dichroic mirror. The included angle is 45° in order to combine the emitted light of different wavelengths. Compared with the structure of the prior art, the present application uses a dichroic mirror to solve the problem of beam combining. Compared with the mechanical structure in the prior art, it has the advantages of compact structure, low cost, and little impact on the external environment temperature change. .

Description of drawings

1 is a schematic structural diagram of a multicolor laser beam combining device in the prior art;

FIG. 2 is a schematic structural diagram of a multi-color laser beam combining device according to an embodiment of the present application.

Correspondence between component names and reference numbers in Figure 1:

Polychromatic laser 110; beam combining prism 120.

Correspondence between component names and reference numbers in Figure 2:

The first sub-laser 210;

Neutron laser 220;

End position sub-laser 230;

Dichroic mirror 240: first mirror surface 241, second mirror surface 242, first anti-reflection film 243, first reflective film 244, first dichroic transflective film 245, second reflective film 246, second dichroic film Transflective film 247, second anti-reflection film 248;

Point to adjustment module 250 .

Detailed ways

Embodiments will be described in detail below, examples of which are illustrated in the accompanying drawings. Where the following description refers to the drawings, the same numerals in different drawings refer to the same or similar elements unless otherwise indicated. The implementations described in the following examples are not intended to represent all implementations consistent with this application. are merely exemplary of systems and methods consistent with some aspects of the present application as recited in the claims.

As shown in FIG. 2 , FIG. 2 is a schematic structural diagram of a multi-color laser beam combining device in an embodiment of the present application.

In an embodiment, as shown in FIG. 2, a multicolor laser beam combining device for flow cytometer includes a multicolor laser and a beam combining prism; it is characterized in that the beam combining prism is a dichroic mirror 240; The color laser includes the first sub-laser 210, the last sub-laser 230, and at least one middle sub-laser 220 between the first sub-laser 210 and the last sub-laser 230; the first sub-laser 210, the last sub-laser 230 and each middle sub-laser 220; The angle between the direction of the emitted light of the sub-laser 220 and the dichroic mirror 240 is 45°, so as to combine the emitted light of different wavelengths. The present application uses one dichroic mirror 240 to solve the problem of beam combining. Compared with the mechanical structure in the prior art, it has the advantages of compact structure, low cost, and little influence on the external environment temperature.

It should be noted that the number of neutron lasers 220 is not limited, and an embodiment of taking only one is introduced herein. It should be thought that when there are multiple neutral position lasers 220, the purpose of beam combining can also be achieved and the technical problem can be solved.

On the basis of the above embodiment, further specific designs for the dichroic mirror 240 can be made.

For example, as shown in FIG. 2 , the dichroic mirror 240 includes a first mirror surface 241 close to the polychromatic laser, and a second mirror surface 242 parallel to the first mirror surface 241; The first anti-reflection film 243 and the second mirror surface 242 are provided with a first reflective film 244 at the position opposite to the first anti-reflection film 243, so that the light emitted by the first sub-laser 210 is transmitted through the first anti-reflection film 243 and then incident on the first anti-reflection film 243. A reflective film 244 performs reflection.

As shown in FIG. 2 , the first mirror surface 241 is provided with a first dichroic transflective film 245 at the position opposite to the mid-position sub-laser 220 , and the second mirror surface 242 is provided with a second dichroic transflective film 245 at the position opposite to the first dichroic transflective film 245 . Two reflective films 246, so that the light emitted by the neutral laser 220 is transmitted through the first dichroic transflective film 245, and the light reflected by the first reflective film 244 is incident on the first dichroic transflective film 245 for reflection, forming two The beams of light are condensed into one beam and incident on the second reflective film 246 . The dichroic transflective film can divide light beams of different wavelengths into transmitted light and reflected light by wavelength.

As shown in FIG. 2 , a second dichroic transflective film 247 is provided at the position where the first mirror surface 241 is opposite to the last sub-laser 230 , and a second dichroic transflective film 247 is provided at the position where the second mirror surface 242 is opposite to the second dichroic transflective film 247 . Second anti-reflection film 248, so that the light emitted by the last sub-laser 230 is transmitted through the second dichroic transflective film 247, and the light reflected by the second reflective film 246 is incident on the second dichroic transflective film 247 for reflection, forming a The two beams of light are converged into one beam and incident on the second anti-reflection film 248 .

In addition, in the above technical solution, the two transflective surfaces of the dichroic mirror 240 need to be <10° parallelism, and the high parallelism ensures the control of the direction of the combined beam, and ensures the transmittance and The reflectivity is not affected by the angle of incidence, and the transmittance or reflectivity of the coating must be greater than 99.5%. Since the beam combining module of the multiple laser channels is a dichroic mirror 240 coated with a transflective film, the phenomenon of pointing drift of the laser due to the influence of the ambient temperature after the beam combining is avoided. The pointing adjustment module 250 can precisely control the pointing of the laser light entering the beam combiner. The pointing adjustment module 250 can be designed as a telephoto lens, and the direction of the laser can be changed by translating the telephoto lens. The longer the focal length of the lens, the higher the precision of adjustment.

Furthermore, a laser shaping module can be added before the laser and the pointing adjustment module 250 to obtain an ideal beam size. The shaping module can be designed according to actual needs, such as adding a telescope to expand or narrow the laser beam. If only one-directional beam expansion or beam reduction is required, it can be designed as a pair of cylindrical mirrors or a pair of prisms.

Similar parts between the embodiments provided in the present application may be referred to each other. The specific embodiments provided above are just a few examples under the general concept of the present application, and do not constitute a limitation on the protection scope of the present application. For those skilled in the art, any other implementations expanded according to the solution of the present application without creative work fall within the protection scope of the present application.

Claims (6)

1. a polychromatic laser beam combining device for a flow cytometer, comprising a polychromatic laser and a beam combining prism; it is characterized in that, the beam combining prism is a dichroic mirror; The first sub-laser, the last sub-laser, and at least one median sub-laser between the first sub-laser and the last sub-laser; the direction of the emitted light of the first sub-laser, the last sub-laser, and each of the median sub-lasers The included angle with the dichroic mirror is 45°, so as to combine the emitted light of different wavelengths. 2 . The polychromatic laser beam combining device for flow cytometry according to claim 1 , wherein the dichroic mirror comprises a first mirror surface close to the polychromatic laser and parallel to the first mirror surface. 3 . the second mirror; The first mirror surface is provided with a first anti-reflection film at the position opposite to the first photon laser, and the second mirror surface is provided with a first reflective film at the position opposite to the first anti-reflection film, so that the first photon The light emitted by the laser is transmitted through the first anti-reflection film, and then incident on the first reflective film for reflection. 3. The multicolor laser beam combining device for flow cytometer according to claim 2, wherein the first mirror surface is provided with a first dichroic transflective film at a position opposite to the neutral sub-laser, The second mirror surface is provided with a second reflective film at a position opposite to the first dichroic transflective film, so that the light emitted by the midpoint laser is transmitted through the first dichroic transflective film, and the The light reflected by the first reflective film is incident on the first dichroic transflective film to be reflected, and the formed two beams of light are converged into one beam and incident on the second reflective film. 4. The multicolor laser beam combining device for flow cytometer as claimed in claim 3, wherein a second dichroic transflective film is provided at the opposite position of the first mirror surface and the last sub-laser, A second anti-reflection film is provided at the position opposite to the second dichroic transflective film on the second mirror surface, so that the light emitted by the last sub-laser is transmitted through the second dichroic transflective film, so the The light reflected by the second reflective film is incident on the second dichroic transflective film to be reflected, and the formed two beams of light are converged into one beam and incident on the second anti-reflection film. 5. The multicolor laser beam combining device for flow cytometer according to any one of claims 1 to 4, wherein a pointing adjustment module is also provided between the multicolor laser and the dichroic mirror . 6 . The multicolor laser beam combining device for flow cytometer according to claim 5 , wherein a laser shaping module is further provided before the pointing adjustment module. 7 .

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