CN209827080U - A laboratory mouse immobilization device for non-exposed tracheal instillation - Google Patents

Abstract

The utility model discloses a laboratory mouse fixing device for non-exposure formula trachea is instiled, including a rectangle inferior gram force board, the contained angle of rectangular plate and horizontal plane is 75-85 degrees, and the rectangular plate size is 15 ~ 20cm 25 ~ 30cm, and the rectangular plate upper end has the string to be used for hanging the last incisor of laboratory mouse, and the rectangular plate lower extreme has the rubber band to be used for the health of fixed rat. The fixture also included an oval ring for opening and holding the mouth of the laboratory mouse, the oval ring having a diameter of 2cm by 4 cm. The utility model discloses with low costs, convenient operation, for the research of medicine lung tissue damage provides convenient, can the wide application in the respiratory tract animal model's of dosing establishment.

Description

A laboratory mouse immobilization device for non-exposed tracheal instillation

technical field

The utility model belongs to the field of auxiliary devices for animal experiments, and relates to a fixation device for experimental rats used for non-exposed trachea instillation.

Background technique

Tracheal instillation is an important exposure method to study lung injury of harmful substances, and it is a commonly used research method in the fields of occupational medicine, environmental medicine and toxicology. In 1923, Kimura first studied the tumorigenicity of coal tar on rabbits and guinea pigs by tracheal instillation. In 1973, researchers first instilled a particle suspension into the lungs of rats through tracheal instillation for related research. In recent years, due to the frequent occurrence of smog, the use of tracheal instillation to study the stress response of PM _2.5 exposure to animals has been widely used. Tracheal instillation can be divided into two types: non-exposed and exposed. The exposed tracheal instillation method refers to cutting the skin and muscles of the neck of the experimental animal to expose the trachea, and then inserting the micro-sample pipette into the trachea. The accuracy of the experiment is guaranteed, but the tip of the micro-sample pipette is relatively Hard, easy to cause respiratory damage in rats. The non-exposed tracheal infusion method uses a blunt intravenous indwelling needle or puncture needle for intubation, and connects a disposable syringe to the intubation needle to achieve tracheal infusion, but the pipeline after the indwelling needle is connected to the syringe is longer, and The dose of tracheal instillation is usually only tens to hundreds of microliters, which makes the method less accurate, and the accuracy of drug exposure or administration is affected to a certain extent, but this method has the advantage of non-invasiveness, and can be injected after instillation. A certain amount of air is injected into the trachea to ensure the smooth breathing of the animal and the uniform dispersion of the infusion solution. Therefore, the non-exposed tracheal instillation method has gradually become the mainstream method in this field in recent years.

In non-exposed tracheal instillation experiments, rodents are commonly used as experimental animals, and the fixation of experimental mice and the accuracy of endotracheal intubation have an important impact on the experimental results. The usual fixation method is to place the animal on a flat plate after anesthesia, and the side of the plate that fixes the experimental animal is at a certain angle, usually an obtuse angle, to the operating table, and then intubated with the help of an operating light. The improved fixation method for the tested animals is to hang the upper incisors of the experimental mice with silk thread after anesthetizing, and let them hang naturally on the self-made plastic frame. Use a pipette gun to absorb the infusion solution, hold the tweezers in the left hand and the pipette gun in the right hand; pull out the tongue of the experimental mouse with the tweezers, inject the infusion solution into the mouth, gently pinch the nasal cavity of the experimental mouse for about 5 seconds, and let it pass through Breathe actively to inhale the infusion solution into the lungs; or directly insert into the trachea for infusion, if you hear wet rales, it means that the infusion is successful. However, the angle at which the experimental rat is fixed in these two methods is not suitable for the experimenter to clearly observe the position of the trachea through the mouth, and the mouth must be opened with the help of tweezers and other instruments to intubate at the same time. Most of the time, the experimenter performs tracheal infusion on the experimental mice based on feeling, which is easy to penetrate the trachea or enter the esophagus by mistake.

Utility model content

The purpose of the utility model is to provide a fixing device for experimental mice, so that the subsequent non-exposed trachea instillation experiment is more convenient and accurate. The utility model develops a fixation device for experimental rats. The side of the fixation plate that fixes the experimental rats is at an acute angle with the operating table so that the experimenter can clearly see the inside of the oral cavity. Combined with the oval oral fixator, the oral cavity can be opened and the tongue can be pressed at the same time. , so that the experimenter does not need to use sharp instruments such as tweezers when intubating, but can clearly see the position of the intubation with the help of the surgical light, realizes visual and accurate intubation, reduces the damage of the experimental mice, and improves the success rate of the experiment.

In order to achieve the above object, the technical solution adopted by the utility model is:

A kind of experimental rat fixation device for non-exposed trachea instillation, comprising a rectangular plate and an oral cavity fixer, the oral cavity fixer is oval ring-shaped, used to open and fix the oral cavity of experimental rats; the rectangular plate The included angle with the horizontal plane is 75-85 degrees; the upper part of the rectangular board is provided with a string for hanging the upper incisors of the experimental rat, and the lower part of the rectangular board is provided with a rubber band for fixing the experimental rat body.

Preferably, the angle between the rectangular plate and the horizontal plane is 80 degrees.

Preferably, the minor axis and the major axis of the elliptical ring are 2 cm and 4 cm, respectively.

Preferably, the material of the rectangular plate is acrylic.

Preferably, the fixing device is further provided with a bottom plate, and the angle between the bottom plate and the rectangular plate is 75-85 degrees.

Preferably, the rectangular plate has a width of 15-20 cm and a height of 25-30 cm.

Preferably, the width and height of the rectangular plates are 20cm and 30cm.

Preferably, the length of the bottom plate is between 15-20 cm, and the width is between 6-8 cm.

Preferably, the oral fixture is detachably connected to the string.

In the existing fixing device, the angle between the side of the rectangular plate fixing the experimental mouse and the operating table is usually an obtuse angle. In the utility model, the angle between the side of the rectangular plate fixing the experimental mouse and the operating table is 75-85 degrees, and the optimal angle is 80 degrees. Hang the upper incisors of the anesthetized experimental rat on the string on the upper part of the rectangular board. The body of the experimental rat is fixed on the rectangular board by the rubber band at the lower end of the rectangular board. From this angle, the experimenter can see the deep part of the oral cavity of the experimental rat. Location. For the convenience of operation, the width of the rectangular acrylic plate is 15-20cm, and the length is 25-30cm.

The oral fixator is an oval ring, and the diameters of the short side and the long side are 2cm and 4cm respectively. There are certain advantages in choosing an oval ring as the oral fixator. Longer, convenient for intubation during tracheal instillation. When in use, the top of the short side is against the upper incisors of the experimental rat, and the bottom of the short side is pressed against the tongue of the experimental rat, so that the oral cavity of the experimental rat is fixed in an O shape.

Experiments have proved that, compared with the prior art, the technical solution in the utility model has the advantages of simplicity, convenience, and strong operability. The utility model has the advantages of low cost and convenient operation, provides convenience for the research of drug lung tissue damage, and can be widely used in the establishment of animal models of respiratory tract administration. The unique design angle of the experimental mouse fixation plate and the oval structure of the experimental mouse oral fixator greatly facilitate the operation of the experimenter, can effectively reduce the mechanical damage to the experimental animals, and improve the accuracy and scientificity of the subsequent infusion experiments. sex.

Description of drawings

Fig. 1 is the schematic diagram of the utility model device.

Detailed ways

The following examples are used to illustrate the present invention, but are not intended to limit the scope of the present invention.

The utility model will be further described below in conjunction with the accompanying drawings and specific embodiments

An experimental mouse immobilization device for non-exposed tracheal instillation (Fig. 1A), including a rectangular acrylic plate a and a base b, the upper end of the rectangular plate has a string 1 for hanging the upper incisors of the experimental mouse, and the lower end of the rectangular plate There are 2 rubber bands for immobilizing the rat's body. An oval ring 3 is also included for opening and fixing the oral cavity of the experimental rat.

The optimal angle (α) between the rectangular plate and the horizontal plane is 80 degrees (Fig. 1B), and it can also be between 75-85 degrees.

The width and height of the rectangular plate are preferably 20cm and 30cm, the width can also be between 15-20cm, the height can also be between 25-30cm, the length of the rectangular plate base is between 15-20cm, and the width is between 6-8cm.

The oral fixator is an oval ring, and the diameters of the short side and the long side are 2cm and 4cm, respectively.

Taking SD rats as the experimental animals, first anesthetize the rats, and then fix them with the experimental mouse fixation device: first hang the upper incisors of the rats on the strings at the top of the fixation board, and then use the rubber bands at the lower end of the rectangular board to fix them. Fix the body of the rat, then use the oval oral fixator to open the rat's mouth, press the tongue, and shine the surgical light on the larynx to fully expose the larynx. At this time, the opening of the trachea can be seen as the rat breathes. Gently insert the tracheal instillation device deep into the trachea, suck the infusion solution with a syringe, push the liquid gently and infuse a small amount of air, with the cooperation of two people, the entire fixation and infusion process can be completed within 30 seconds . The whole operation process including anesthesia, fixation and infusion can be completed within 1-2 minutes. Ether anesthesia and intravenous anesthesia can meet the requirements for tracheal instillation of small animals.

The device of the utility model is used to expose 70 SD rats by tracheal drip infusion. PM _2.5 , the infusion volume is 1mL/kg body weight, wet rales can be heard during the infusion process, indicating that the infusion is successful, and none of the 70 rats died after the infusion, indicating that the experimental mouse fixation device is used for tracheal instillation Note that the exposure experiment has the advantages of fast and convenient, low cost, and less trauma to animals.

Claims (9)

1. The utility model provides a laboratory mouse fixing device for non-exposure formula trachea is instilled, includes a rectangular plate and an oral cavity fixer, its characterized in that: the oral cavity fixer is in an elliptical ring shape; the included angle between the rectangular plate and the horizontal plane is 75-85 degrees; the upper part of the rectangular plate is provided with a string used for hanging the upper incisor of the laboratory mouse, and the lower part of the rectangular plate is provided with a rubber band used for fixing the laboratory mouse body.

2. The laboratory mouse fixation device for non-exposed tracheal instillation of claim 1, wherein: the included angle between the rectangular plate and the horizontal plane is 80 degrees.

3. The laboratory mouse fixation device for non-exposed tracheal instillation of claim 1, wherein: the minor axis and the major axis of the elliptical ring are respectively 2cm and 4 cm.

4. The laboratory mouse fixation device for non-exposed tracheal instillation of claim 1, wherein: the rectangular plate is made of acrylic.

5. The laboratory mouse fixation device for non-exposed tracheal instillation of claim 1, wherein: the fixing device is also provided with a bottom plate, and the included angle between the bottom plate and the rectangular plate is 75-85 degrees.

6. The laboratory mouse fixation device for non-exposed tracheal instillation of claim 1, wherein: the width of the rectangular plate is 15-20cm, and the height of the rectangular plate is 25-30 cm.

7. The laboratory mouse fixation device for non-exposed tracheal instillation of claim 1, wherein: the width and height of the rectangular plate were 20cm and 30 cm.

8. The laboratory mouse fixation device for non-exposed tracheal instillation of claim 1, wherein: the length of the bottom plate is between 15 and 20cm, and the width of the bottom plate is between 6 and 8 cm.

9. The laboratory mouse fixation device for non-exposed tracheal instillation of claim 1, wherein: the oral cavity fixer is detachably connected with the string.