CN104586531B - Rapid inhalation type intratracheal instillation medication system - Google Patents

Abstract

The rapid inhalation tracheal infusion drug delivery system, the delivery system includes: an anesthesia device, used to anesthetize the subject of drug administration; a fixation device, used to fix the subject of drug administration after anesthesia; Intratracheal instillation of drugs in drug subjects. The rapid inhalation tracheal instillation system and method of the present invention have many advantages such as low cost, convenient operation, non-invasive high efficiency, and environmental friendliness, and can be widely applied to respiratory drug administration or drug exposure experiments and the establishment of various disease models.

Description

Rapid inhalation tracheal drip drug delivery system

technical field

The present invention relates to a drug delivery system and method, more specifically, to a rapid inhalation tracheal instillation drug delivery system and method.

Background technique

Respiratory exposure is an important exposure route in research fields such as occupational medicine, environmental medicine, and toxicology. Respiratory exposure and respiratory drug administration have thus become indispensable means for animal experimental research. In 1973, relevant scholars reported for the first time that the particle suspension was successfully dripped into the lungs of rats by using tracheal intubation, and tracheal intubation has gradually become the main experimental method for drug administration or exposure through the trachea. In the past, the exposed tracheal instillation needed to incise the skin and muscle of the neck of the rat to expose the trachea, and then insert the micro sample injector into the trachea, so that the accuracy of the experiment was guaranteed, but the pointed micro sample injection The device is easy to cause respiratory damage and pain in rats. Today's non-exposed tracheal instillation is cannulated with a blunt intravenous catheter or needle, which has the advantage of being noninvasive but is less precise. Because these cannula needles tend to fit into normal syringes, which are not as precise as microsamplers. In conclusion, non-invasive and precise tracheal instillation devices and methods are difficult to achieve. Studies have shown that nasal instillation can also achieve the purpose of drug administration or poisoning in the respiratory tract, but the speed of administration is slow, and the volatile gas anesthesia method characterized by rapid anesthesia and rapid recovery cannot meet its requirements.

Contents of the invention

The purpose of the present invention is to provide a rapid inhalation type tracheal instillation drug delivery system and method for some and certain problems in the prior art.

According to a first aspect of the present invention, a rapid inhalation tracheal infusion drug delivery system is provided, comprising:

an anesthesia device for anesthetizing the subject;

An immobilization device for immobilizing the subject after anesthesia; and

The drug delivery device is used for instilling medicine into the trachea of the drug delivery subject.

Preferably, wherein the anesthesia device comprises:

The remote control car is equipped with a carrier, and the remote control car is used to carry the drug administration object;

Two-stage anesthesia cabin, including a front cabin and a rear cabin, the front cabin is provided with a front cabin door, and the rear cabin is provided with a rear cabin door, and the front cabin and the rear cabin are sealedly connected through the rear cabin door; the rear cabin is used contain volatile anesthetics; and

The remote control can control the remote control car to automatically enter and exit the front cabin and the rear cabin through the front hatch and the rear hatch.

More preferably, wherein the front cabin is connected with a vacuum device and a filtering device.

More preferably, wherein both the front hatch and the rear hatch are automatic remote control doors.

Preferably, the fixing device includes a workbench and a tether fixed on the workbench, the workbench has a vertical table, and the upper incisors of the anesthetized subject can be tied by the tether, so that the subject can work along the The vertical table top of the table hangs down naturally. More preferably, the immobilization device further includes a clamper, which is used to clamp and pull out the tongue of the administration subject. More preferably, one end of the holder is fixed on the vertical table of the workbench.

Preferably, wherein the drug delivery device comprises a pipette.

Preferably, the subject of administration is a rat or a mouse.

According to a second aspect of the present invention, a rapid inhalation tracheal drip administration method is provided, comprising the steps of:

(1) Put the subject to be administered into an anesthesia cabin filled with volatile anesthetics. When the subject falls down and does not move, breathing deeply and slowly as if sleeping, the anesthesia is completed;

(2) Use a tether to hang the upper incisors of the subject to be administered, so that it hangs naturally on the workbench, clamp and pull out the subject's tongue with a holder;

(3) Draw the infusion solution with a pipette gun, and inject the infusion solution into the mouth of the subject;

(4) Gently pinch the nasal cavity of the recipient with hands for about 5 seconds, so that the infusion solution can be actively inhaled into the lungs through breathing. If wet crackles are heard, the infusion is successful.

The rapid inhalation tracheal instillation system and method of the present invention have many advantages such as low cost, convenient operation, non-invasive high efficiency, and environmental friendliness, and can be widely applied to respiratory drug administration or drug exposure experiments and the establishment of various disease models.

Description of drawings

Fig. 1 is the schematic diagram of the anesthesia device of drug delivery system of the present invention; And

Fig. 2 is a schematic diagram of the fixing device of the drug delivery system of the present invention.

detailed description

The rapid inhalation tracheal drip drug delivery system and method of the present invention will be described in detail below with reference to the accompanying drawings. Those skilled in the art should understand that the embodiments described below are only illustrative illustrations of the present invention, and are not intended to limit it in any way.

According to the rapid inhalation type tracheal drip drug delivery system (device) of the present invention, it mainly includes: an anesthesia device, a fixing device and a drug delivery device. Wherein, the anesthesia device is used to anesthetize the subject of drug administration; the fixation device is used to fix the subject of drug administration after anesthesia; the drug delivery device is used to instill medicine into the trachea of the subject of drug administration. The subject of administration can be any rodent.

Referring to Fig. 1, in a specific embodiment of the present invention, the anesthesia device includes: a remote control car 5, a two-stage anesthesia cabin and a remote control (not shown in the figure). The remote control car 5 is provided with a carrier for carrying the subject 6 to be administered. The carrier is usually a cage, which can facilitate the inhalation anesthesia of the subject 6 and observe the state of the subject 6 in real time. The two-stage anesthesia cabin includes a front cabin 3 and a rear cabin 4, the front cabin 3 is provided with a front cabin door 1, and there is a sealing structure between the front cabin door 1 and the front cabin 3; the rear cabin 4 is provided with a rear cabin door 2, and the rear cabin There is also a sealing structure between the door 2 and the rear compartment 4; the front compartment 3 and the rear compartment 4 are sealably connected through the rear compartment door 2. When the front cabin door 1 and the rear cabin door 2 are closed, the front cabin 3 and the rear cabin 4 can each form an airtight seal with the outside world. The rear compartment 4 is used to accommodate the volatile anesthetic, so as to perform rapid inhalational anesthesia on the subject 6 to be administered. The volatile anesthetic can be ether or isoflurane, which can quickly anesthetize and wake up the subject 6, and will not Caused serious damage to Subject 6. The remote controller can control the remote control car 5 to enter and exit the front compartment 3 and the rear compartment 4 automatically through the front hatch 1 and the rear hatch 2 . Front cabin 3 and rear cabin 4 all have transparent material (such as inorganic or plexiglass) to make usually, thereby can observe the situation in the cabin in real time from the outside, convenient remote control operation. The above-mentioned anesthesia device is not only applicable to the drug delivery system of the present invention, but also applicable to other experimental systems.

When the remote control car 5 enters and exits the rear cabin 4 from the front cabin 3, a small part of the volatile anesthetic in the rear cabin 4 will volatilize in the front cabin 3 through the rear cabin door 2, so in some embodiments of the present invention, the front cabin The cabin 3 is connected with a vacuuming device and a filtering device (not shown in the figure), so that the anesthetic agent volatilized into the front cabin 3 is vacuumed and filtered, so that the indoor environment and the operator will not be polluted. Wherein, the front cabin 3 is also provided with an air intake valve (not shown in the figure). After the vacuuming is completed, the front cabin 3 can be inflated through the air intake valve. After being inflated to atmospheric pressure, the front cabin door 1 can be opened. The remote control car 5 is driven out of the front compartment 3.

In some embodiments of the present invention, both the front cabin door 1 and the rear cabin door 2 can be automatic remote control doors, and their controllers can be integrated into the remote controller of the remote control car 5, so that the front cabin can be automatically controlled by the remote controller. Opening and closing of door 1 and rear hatch 2. The principle and structure of the automatic remote control door and the remote control are well known to those skilled in the art, and further description thereof is omitted here.

Referring to Fig. 2, fixing device among the present invention comprises workbench (frame) 9 and the tether 8 that is fixed on workbench 9, and workbench 9 has vertical table top, and the administration object 6 after the anesthesia can be passed through tether 8 The upper incisors are fastened, so that the drug administration object 6 naturally sags along the vertical table top of the workbench 9. The fixation device may also include a clamper 7, which is used to clamp and pull out the tongue of the subject 6 for administration. One end of the holder 7 can be fixed on the vertical table surface of the workbench 9 . The drug delivery device is used to instill medicine into the oral cavity of the subject 6. The drug delivery device may be a pipette gun, which has higher drug delivery accuracy than a syringe.

The rapid inhalation tracheal instillation administration method of the present invention will be specifically described below by taking mice as administration objects.

The rapid inhalation type tracheal drip administration method of the present invention comprises the following:

(1) Put the mouse into an anesthesia device equipped with a volatile anesthetic. When the mouse falls down and does not move, breathing deeply and slowly as if sleeping, the anesthesia is completed;

(2) Hang the mouse's upper incisors with a tether, make it hang naturally on the workbench, clamp and pull out the mouse's tongue with a gripper;

(3) Draw the infusion solution with a pipette gun, and inject the infusion solution into the mouth of the mouse;

(4) Gently pinch the nasal cavity of the mouse for about 5 seconds to make it actively inhale the infusion solution into the lungs through breathing. If a wet rale is heard, the infusion is successful.

Wherein, the anesthesia device in the above step (1) preferably adopts the anesthesia device described above with reference to FIG. Open the front cabin door 1, and control the remote control car 5 to enter the front cabin 3. Close the front hatch 1 and open the rear hatch 2. Control the remote control car 5 to enter the rear cabin 4, and close the rear cabin door 2 quickly. After the mouse 6 is anesthetized in the rear cabin 4, the rear cabin door 2 is opened. Control the remote control car 5 to enter the front cabin 3, and close the rear cabin door 2 quickly. Vacuumize the front cabin 3, and process a small amount of volatile anesthetic that enters the front cabin 3. After the vacuum degree in the front cabin 3 reaches the set value, stop vacuuming. Open the suction valve to make the front cabin 3 return to normal pressure. Open the front cabin door 1, control the remote control car 5 to drive out of the front cabin 3, and the anesthesia operation is completed.

Among them, the above-mentioned steps (1) and (2) take a relatively long time, so they can be completed by assistants, and the steps (3) and (4) take a relatively short time, and require high accuracy of drug administration, so they can be It is done by experienced professionals, which can save the time of professionals and ensure the success rate of drug administration.

Taking 12 mice as an example, the drug administration method of the present invention was used for drug infusion. After the instillation, the mice can quickly return to normal state, and there is no adverse effect on the diet and other activities of the mice, and the success rate is 100%.

By comparing with other respiratory drug delivery or drug exposure devices, the advantages of the rapid inhalation tracheal instillation drug delivery system and method of the present invention are summarized as follows:

(1) The system cost is low and the economy is reasonable. (2) The operation is simple, no intubation is required, the animal is suspended naturally, the pain and discomfort of the animal are reduced, and it is easy to master and popularize. (3) The use of inhalation anesthesia takes a short time, and it is easy to adjust the depth of anesthesia and terminate anesthesia quickly. After the infusion, the animals wake up quickly without affecting the normal physiological activities. (4) The infusion effect is good and the success rate is high. (5) The anesthesia operation is automatically sealed and will not affect the operator and the indoor environment.

In summary, the rapid inhalation tracheal instillation system and method of the present invention have many advantages such as low cost, convenient operation, non-invasive efficiency, and environmental friendliness. Establish.

Claims (8)

1. a kind of quick suction-type tracheal instillation administration system, including：

Anesthesia outfit, for anaesthetizing administration object；

Fixing device, for being fixed to postanesthetic administration object；And

Doser, for the intratracheal instillation medicine to administration object；

Wherein anesthesia outfit includes：

Remote operated vehicle, is provided with carrier, and remote operated vehicle is used for delivering administration object；

Two-part anaesthetizes cabin, and including front deck and rear deck, front deck is provided with front door, and rear deck is provided with rear door, front deck and rear deck it Between sealably connected by rear door；Wherein rear deck is used for accommodating volatile anesthetic；And

Remote control, can control that remote operated vehicle passes through front door and rear door passes in and out front deck and rear deck automatically.

2. quick suction-type tracheal instillation administration system according to claim 1, wherein front deck is connected with vacuum extractor And defecator.

3. quick suction-type tracheal instillation administration system according to claim 1, wherein front door and rear door are all certainly Dynamic remote control gate.

4. quick suction-type tracheal instillation administration system according to claim 1, wherein fixing device include workbench and Fixing tether on the table, workbench has vertical table top, being capable of visiting postanesthetic administration object by tether Dentition is lived, and so that administration object is naturally drooped along the vertical table top of workbench.

5. quick suction-type tracheal instillation administration system according to claim 4, fixing device also includes clamper, clamping Device is used for clamping and pull out the tongue of administration object.

6. quick suction-type tracheal instillation administration system according to claim 5, wherein clamper one end is fixed on work On the vertical table top of platform.

7. quick suction-type tracheal instillation administration system according to claim 1, wherein doser includes liquid-transfering gun.

8., according to the arbitrary described quick suction-type tracheal instillation administration system of claim 1-7, wherein administration object is rat Or mice.