CN209778864U - microorganism detection culture apparatus - Google Patents
microorganism detection culture apparatus Download PDFInfo
- Publication number
- CN209778864U CN209778864U CN201920533897.2U CN201920533897U CN209778864U CN 209778864 U CN209778864 U CN 209778864U CN 201920533897 U CN201920533897 U CN 201920533897U CN 209778864 U CN209778864 U CN 209778864U
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- CN
- China
- Prior art keywords
- filter membrane
- annular
- bin
- filter
- membrane seat
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000001514 detection method Methods 0.000 title claims abstract description 33
- 244000005700 microbiome Species 0.000 title claims description 28
- 239000012528 membrane Substances 0.000 claims abstract description 70
- 239000007788 liquid Substances 0.000 claims abstract description 41
- 238000002955 isolation Methods 0.000 claims abstract description 24
- 229920001817 Agar Polymers 0.000 claims abstract description 19
- 239000008272 agar Substances 0.000 claims abstract description 19
- CBENFWSGALASAD-UHFFFAOYSA-N Ozone Chemical compound [O-][O+]=O CBENFWSGALASAD-UHFFFAOYSA-N 0.000 claims abstract description 17
- 241000894006 Bacteria Species 0.000 claims abstract description 16
- 238000012360 testing method Methods 0.000 claims abstract description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- 238000007789 sealing Methods 0.000 claims description 11
- 239000000725 suspension Substances 0.000 claims description 6
- 239000002699 waste material Substances 0.000 claims description 6
- 230000001580 bacterial effect Effects 0.000 claims description 5
- 238000011010 flushing procedure Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 abstract description 32
- 230000002906 microbiologic effect Effects 0.000 abstract description 7
- 241000233866 Fungi Species 0.000 abstract description 5
- 230000000813 microbial effect Effects 0.000 abstract description 2
- 239000003814 drug Substances 0.000 description 7
- 229940079593 drug Drugs 0.000 description 6
- 238000000034 method Methods 0.000 description 6
- 238000005406 washing Methods 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000005374 membrane filtration Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 208000030453 Drug-Related Side Effects and Adverse reaction Diseases 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 108010066057 cabin-1 Proteins 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 238000009629 microbiological culture Methods 0.000 description 1
- 238000011085 pressure filtration Methods 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a microbial detection culture apparatus, including keeping apart operation storehouse, operating gloves, collection fungus filter, test reagent hang the hook, wash the bag and hang hook, ozone device, vacuum pump and be equipped with the culture dish of agar culture medium. The utility model discloses microbiological detection culture apparatus, it filters album fungus and all goes on in aseptic isolation operation storehouse with the operation that filtration membrane transferred to on the agar plate, the operation of having avoided in open space is introduced the bacterium and is caused the unsafe technical problem of testing result, and adopt the cooperation of table surface between annular filtration membrane seat and filter bowl and the collection liquid cup, and utilize the spring clamping to annular filtration membrane seat, filter bowl and collection liquid cup are connected fixedly, make equipment between the three with dismantle convenient and fast, can be convenient take off filter bowl and filtration membrane seat after filtering collection fungus and accomplishing, the technical problem of getting with tweezers and putting filtration membrane troublesome poeration and introducing the bacterium has been avoided.
Description
Technical Field
The utility model relates to a microbiological detection technical field, in particular to microbiological detection culture apparatus.
Background
Microbial contamination is one of the main causes of adverse drug events, prevents contaminated drugs from flowing into the market, and has great significance for guaranteeing the life safety of patients. The membrane filtration technology is an important technology for attaching and collecting microorganisms in the field of drug microorganism inspection. For the drugs with antibacterial activity, a membrane filtration method is needed to fully eliminate the antibacterial activity of the drugs so as to facilitate the recovery, reproduction and detection of microorganisms in dormant or damaged states in the drugs.
The existing filtering bacteria collector needs to transfer a filtering membrane to an agar plate for culture after filtering and collecting bacteria for a test drug so as to detect the total number of bacterial colonies. However, in the prior art, the filtering and bacteria collecting operation and the transferring and filtering membrane are all performed in an open space, and in the transferring and filtering membrane process, the filtering membrane needs to be taken down from the base of the filtering cup by using tweezers, the operation is complex, bacteria are easily introduced in the operation process to cause pollution, and the accuracy of the detection result is further influenced.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing a microbiological detection culture apparatus to solve among the prior art and all carry out in open space in the operation of filtering collection fungus and transferring filtration membrane to the agar plate, the operation is complicated that the filtration membrane need be got with tweezers clamp, introduces the thin appearance in the operation process and causes the unsafe technical problem of testing result.
The utility model discloses a microorganism detection and culture device, which comprises an isolation operation bin consisting of a bin body and a bin door, wherein the bin body or the bin door is provided with an operating hand inlet, and the operating hand inlet is connected with an operating glove;
A bacteria collection filter is arranged in the isolation operation bin and comprises a filter bowl, a liquid collection cup, an annular filter membrane seat arranged between the filter bowl and the liquid collection cup, a filter membrane arranged on the filter membrane seat and a first support detachably connected with the liquid collection cup;
The upper end and the lower end of the annular filter membrane seat are in a circular truncated cone shape, an annular groove is formed in the inner side of the upper end of the annular filter membrane seat, and the filter membrane is arranged in the annular groove;
A first annular flange is arranged at the lower end of the filter cup, and a first inner chamfer matched with the upper end circular table surface of the annular filter membrane seat is arranged at the lower port of the first annular flange;
A second annular flange is arranged at the upper end of the liquid collecting cup, and a second inner chamfer matched with a circular table surface at the lower end of the annular filter membrane seat is arranged at the upper port part of the second annular flange; the bottom of the liquid collecting cup is also provided with a liquid outlet;
The microorganism detection and culture device also comprises a spring clip for clamping and fixing the first annular flange and the second annular flange.
A test reagent suspension hook and a flushing bag suspension hook are arranged at the top of the inner side of the isolation operation bin, a sealing cover is arranged at the upper end of the filter cup, and a liquid inlet is formed in the sealing cover;
the microorganism detection and culture device also comprises a drainage tube, wherein the drainage tube comprises a tube body, a puncture needle arranged at one end of the tube body and a sealing joint arranged at the other end of the tube body and used for being sleeved on the liquid inlet;
The microorganism detection and culture device also comprises an ozone device and a vacuum pump which are arranged outside the isolated operation bin; the ozone device is connected with the isolation operation bin through an air pipe and is used for inputting ozone into the isolation operation bin; the vacuum pump is connected with a waste liquid bottle through a pipe, and the waste liquid bottle is connected with a liquid collecting cup in the operation isolation bin through a pipe;
the isolation operation bin is also provided with an air inlet, and the air inlet is provided with a bacterial filter;
the microorganism detection culture device also comprises a second support used for placing the annular filter membrane seat and a culture dish filled with agar culture medium, and the second support is positioned beside the first support; and a third inner chamfer matched with the lower end circular table surface of the annular filter membrane seat is arranged on the second support, and a fourth inner chamfer matched with the upper end circular table surface of the annular filter membrane seat is arranged at the mouth part of the culture dish.
further, a handle is arranged on the side face of the spring clamp.
The utility model has the advantages that:
1. The utility model discloses microbiological detection culture apparatus, its filtration collection fungus and the operation that will filter membrane and transfer to on the agar plate all go on in aseptic isolation operation storehouse, have avoided the operation to introduce the bacterium in open space and have caused the unsafe technical problem of testing result.
2. The utility model discloses microbiological detection culture apparatus, adopt the mesa cooperation between its annular filter membrane seat and filter bowl and the catch bowl, and utilize the spring clamping to annular filter membrane seat, filter bowl and catch bowl are connected fixedly, make equipment between the three with dismantle convenient and fast, can be convenient take off filter bowl and filter membrane seat after filtering the catch bowl and accomplish, then will filter the membrane seat and place on the second support, invert agar on filtration membrane again, can carry out the microbiological culture, the technical problem of getting with tweezers and putting filtration membrane troublesome poeration and introducing the bacterium has been avoided.
3. The utility model discloses microbiological detection culture apparatus adopts the mesa cooperation between its annular filter membrane seat and filter bowl and the catch bowl, and the leakproofness is good between the fitting surface.
drawings
FIG. 1 is a schematic view showing the structure of a microorganism detection culture apparatus according to an embodiment;
FIG. 2 is a schematic structural view of an isolation operation bin;
FIG. 3 is a schematic view of the bacteria-collecting filter;
FIG. 4 is a schematic structural view of a filter bowl;
FIG. 5 is a schematic structural view of the liquid collecting cup;
FIG. 6 is a schematic view of the structure of the filter membrane transferred to the second support.
Detailed Description
The invention is further described with reference to the following figures and examples.
As shown in the figure, the microorganism detection and culture device of the present embodiment includes an isolation operation cabin 1 composed of a cabin body 101 and a cabin door 102, wherein an operator inlet 2 is arranged on the cabin body or the cabin door, and an operation glove 3 is connected to the operator inlet.
A bacteria collection filter is arranged in the isolation operation bin and comprises a filter bowl 4, a liquid collection bowl 5, an annular filter membrane seat 6 arranged between the filter bowl and the liquid collection bowl, a filter membrane 7 arranged on the filter membrane seat and a first support 8 detachably connected with the liquid collection bowl; in this embodiment, collection liquid cup bottom is provided with rectangle inserted bar 9, and collection liquid cup passes through rectangle inserted bar cartridge on the support, easy dismounting.
The upper end and the lower extreme of annular filter membrane seat are round platform shape, the upper end inboard of annular filter membrane seat is provided with the annular groove, filtration membrane sets up in the annular groove. In a specific implementation, the top surface of the filter membrane may be slightly higher than the end surface of the annular groove, which allows good contact of the bacteria with the agar when the filter membrane is transferred onto the agar.
the lower extreme of filter bowl is provided with first annular flange 10, the lower port portion of first annular flange is provided with the first interior chamfer 11 with the upper end mesa complex of annular filter membrane seat.
A second annular flange 12 is arranged at the upper end of the liquid collecting cup, and a second inner chamfer 13 matched with a circular table surface at the lower end of the annular filter membrane seat is arranged at the upper port part of the second annular flange; the bottom of the liquid collecting cup is also provided with a liquid outlet 14.
the microorganism detection and culture device further comprises a spring clip 15 for clamping and fixing the first annular flange and the second annular flange.
The top of the inner side of the isolation operation bin is provided with a reagent suspension hook 16 and a washing bag suspension hook 17, the upper end of the filter cup is provided with a sealing cover 18, and the sealing cover is provided with a liquid inlet 19.
The microorganism detection and culture device further comprises a drainage tube 20, wherein the drainage tube comprises a tube body 201, a puncture needle 202 arranged at one end of the tube body, and a sealing joint 203 arranged at the other end of the tube body and used for being sleeved on the liquid inlet.
The microorganism detection and culture device also comprises an ozone device 21 and a vacuum pump 22 which are arranged outside the isolated operation bin; the ozone device is connected with the isolation operation bin through an air pipe and is used for inputting ozone into the isolation operation bin; the vacuum pump is connected with a waste liquid bottle 23 through a pipe, and the waste liquid bottle is connected with a liquid outlet at the bottom of the liquid collecting cup through a pipe. The ozone device described in this embodiment may be an ozone bottle or an ozone generator.
the isolation operation cabin is also provided with an air inlet, and the air inlet is provided with a bacteria filter 24.
the microorganism detection culture device also comprises a second support 25 for placing the annular filter membrane seat and a culture dish 27 filled with an agar culture medium 26, wherein the second support is positioned beside the first support; and a third inner chamfer matched with the lower end circular table surface of the annular filter membrane seat is arranged on the second support, and a fourth inner chamfer matched with the upper end circular table surface of the annular filter membrane seat is arranged at the mouth part of the culture dish.
the operation process of the microorganism detection and culture device of the embodiment is as follows:
The first step is as follows: hanging the test reagent and the washing bag on corresponding hooks in the isolated operation bin, and putting the drainage tube and the culture dish filled with agar into the operation bin;
Secondly, closing a bin door, starting an ozone device to input ozone into the bin, and sterilizing the bin by using the ozone;
thirdly, after the sterilization treatment, starting a vacuum pump to pump out the gas in the bin so as to eliminate the ozone in the bin, wherein in the process of pumping out the gas in the bin, the outside air can enter the bin through a bacterial filter on the isolation operation bin, and the air entering the bin is clean and sterile due to the filtering action of the bacterial filter;
and fourthly, putting the two hands of an operator into the operating gloves for operation, connecting the sealing joint at one end of the drainage tube with the liquid inlet, piercing the puncture needle at the other end of the drainage tube into the test medicine bag, and then starting the vacuum pump to enable the two sides of the filtering membrane to form pressure difference so as to realize negative pressure filtering.
and fifthly, after the filtration is finished, the puncture needle is punctured into the washing bag to wash the filtration membrane so as to eliminate the influence of the bacteriostatic substance on the microorganisms.
And sixthly, taking down the spring clamp, detaching the filter cup, taking the filter membrane seat down, transferring the filter membrane seat to a second support, opening the package of the agar culture dish, reversely buckling the agar culture dish on the filter membrane, and clamping the spring clamp on the agar culture dish and the second support so as to fix the agar culture dish and the annular filter membrane seat, so that bacteria on the filter membrane can propagate, grow and propagate on agar.
In the microorganism detection culture device in the embodiment, the operations of filtering and collecting bacteria and transferring the filtering membrane to the agar plate are carried out in the sterile isolation operation cabin, so that the technical problem of inaccurate detection results caused by introducing bacteria in an open space is solved.
microorganism detects culture apparatus in this embodiment, adopt the mesa cooperation between its annular filter membrane seat and filter bowl and the catch bowl, and utilize the spring clamping to annular filter membrane seat, filter bowl and catch bowl are connected fixedly, make equipment between the three with dismantle convenient and fast, can be convenient take off filter bowl and filter membrane seat after filtering the catch bowl and accomplish, then place the filter membrane seat on the second support, invert agar on filtration membrane again, can carry out the microorganism culture, avoided getting with tweezers and put the troublesome technical problem of filtration membrane operation.
In the microorganism detection and culture device in the embodiment, the annular filter membrane seat is matched with the filter cup and the liquid collecting cup by the circular table surface, the sealing performance between matching surfaces is good, and the smooth proceeding of negative pressure filtration is ensured.
In this embodiment, a handle 28 is disposed on a side surface of the spring clip, and the handle is disposed to improve the convenience of operation of the spring clip.
Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.
Claims (2)
1. A microorganism detection culture apparatus, characterized in that: the device comprises an isolated operation bin consisting of a bin body and a bin door, wherein an operating hand inlet is formed in the bin body or the bin door, and operating gloves are connected to the operating hand inlet;
a bacteria collection filter is arranged in the isolation operation bin and comprises a filter bowl, a liquid collection cup, an annular filter membrane seat arranged between the filter bowl and the liquid collection cup, a filter membrane arranged on the filter membrane seat and a first support detachably connected with the liquid collection cup;
The upper end and the lower end of the annular filter membrane seat are in a circular truncated cone shape, an annular groove is formed in the inner side of the upper end of the annular filter membrane seat, and the filter membrane is arranged in the annular groove;
A first annular flange is arranged at the lower end of the filter cup, and a first inner chamfer matched with the upper end circular table surface of the annular filter membrane seat is arranged at the lower port of the first annular flange;
A second annular flange is arranged at the upper end of the liquid collecting cup, and a second inner chamfer matched with a circular table surface at the lower end of the annular filter membrane seat is arranged at the upper port part of the second annular flange; the bottom of the liquid collecting cup is also provided with a liquid outlet;
the microorganism detection and culture device also comprises a spring clamp used for clamping and fixing the first annular flange and the second annular flange;
A test reagent suspension hook and a flushing bag suspension hook are arranged at the top of the inner side of the isolation operation bin, a sealing cover is arranged at the upper end of the filter cup, and a liquid inlet is formed in the sealing cover;
The microorganism detection and culture device also comprises a drainage tube, wherein the drainage tube comprises a tube body, a puncture needle arranged at one end of the tube body and a sealing joint arranged at the other end of the tube body and used for being sleeved on the liquid inlet;
the microorganism detection and culture device also comprises an ozone device and a vacuum pump which are arranged outside the isolated operation bin; the ozone device is connected with the isolation operation bin through an air pipe and is used for inputting ozone into the isolation operation bin; the vacuum pump is connected with a waste liquid bottle through a pipe, and the waste liquid bottle is connected with a liquid collecting cup in the operation isolation bin through a pipe;
The isolation operation bin is also provided with an air inlet, and the air inlet is provided with a bacterial filter;
the microorganism detection culture device also comprises a second support used for placing the annular filter membrane seat and a culture dish filled with agar culture medium, and the second support is positioned beside the first support; and a third inner chamfer matched with the lower end circular table surface of the annular filter membrane seat is arranged on the second support, and a fourth inner chamfer matched with the upper end circular table surface of the annular filter membrane seat is arranged at the mouth part of the culture dish.
2. the microorganism detection culture apparatus according to claim 1, wherein: and a handle is arranged on the side surface of the spring clamp.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920533897.2U CN209778864U (en) | 2019-04-18 | 2019-04-18 | microorganism detection culture apparatus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201920533897.2U CN209778864U (en) | 2019-04-18 | 2019-04-18 | microorganism detection culture apparatus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN209778864U true CN209778864U (en) | 2019-12-13 |
Family
ID=68803266
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201920533897.2U Expired - Fee Related CN209778864U (en) | 2019-04-18 | 2019-04-18 | microorganism detection culture apparatus |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN209778864U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113789252A (en) * | 2021-08-31 | 2021-12-14 | 广州市顺元医疗器械有限公司 | Microbial filtration device, full-automatic little biological collection detection device |
| CN116075586A (en) * | 2020-07-08 | 2023-05-05 | 快速微型生物系统公司 | Filtration assemblies, cartridges, systems and methods for filtration and cell growth |
| EP4159836A4 (en) * | 2020-05-29 | 2024-07-17 | Hitachi High-Tech Corporation | FILTER CARTRIDGE AND MICROBIAL TESTING PROCEDURE |
-
2019
- 2019-04-18 CN CN201920533897.2U patent/CN209778864U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP4159836A4 (en) * | 2020-05-29 | 2024-07-17 | Hitachi High-Tech Corporation | FILTER CARTRIDGE AND MICROBIAL TESTING PROCEDURE |
| CN116075586A (en) * | 2020-07-08 | 2023-05-05 | 快速微型生物系统公司 | Filtration assemblies, cartridges, systems and methods for filtration and cell growth |
| CN113789252A (en) * | 2021-08-31 | 2021-12-14 | 广州市顺元医疗器械有限公司 | Microbial filtration device, full-automatic little biological collection detection device |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20191213 Termination date: 20200418 |