CN209745989U - Qualitative/quantitative biochip detection device - Google Patents

Abstract

The utility model discloses a qualitative/quantitative biochip detection device, characteristics are including detecting the box and detecting the box, are provided with image display mechanism on the detection box, are provided with biochip reaction mechanism, light source and image capture mechanism in the detection box, and image capture mechanism is connected with image display mechanism electricity. The advantage is that above-mentioned detection device is integrated in a detection box with biochip's reaction and detection, and the back is accomplished in the reaction, provides the light source for biochip when detecting through the light source, then catches the image information on the biochip and sends the image information who catches to image display mechanism through image capture mechanism, and simple structure, portability are good and can obtain comparatively accurate observation result.

Description

A qualitative/quantitative biochip detection device

technical field

The utility model relates to the field of biochip detection, in particular to a qualitative/quantitative biochip detection device.

Background technique

Biochip (biochip or bioarray) integrates the biochemical analysis process on the surface of the chip based on the principle of specific interaction between biomolecules, so as to realize high-throughput and rapid detection of DNA, RNA, peptides, proteins and other biological components. The narrow concept of biochip refers to the immobilization of biomolecules (oligonucleotides, cDNA, genomicDNA, polypeptides, antibodies, antigens, etc.) on silicon chips, glass chips (beads), plastic chips (beads), gels, Biomolecular lattices formed on solid-phase transmitters such as nylon membranes. The concept of chip is derived from the concept of integration, and biochip is the integration of biological materials. Like laboratory testing, checking blood sugar, protein, enzyme activity, etc. on a biochip is based on the same biological reaction principle. Therefore, a biochip is a carrier platform, a carrier, and a miniature biochemical analysis system that integrates discontinuous analysis processes in the field of life sciences on the surface of the substrate to achieve accurate detection of cells, proteins, genes, and other biological components. , Fast and large amount of information detection. The technical advantages of biochips make detection more rapid, sensitive and accurate.

Conventional biochip detection first needs to react to biological samples. After the reaction is completed, there are currently two main methods for specific detection and research on biochips. One is to use a microscope to observe the biochip with the naked eye of the human body, and then according to The observed results are analyzed and studied; the other is to scan the biochip through a scanner, input the scanned results into the computer, observe through the computer, and conduct in-depth research based on the observation results. The above two methods mainly have the following problems: the former uses a microscope to observe the biochip with the naked eye of the human body, but it is difficult to record the observation results, and can only draw a sketch of the observation results while observing, which requires high drawing ability of the observer , so this detection and research method is not only inconvenient to record the observation results, but also difficult to guarantee the accuracy of the observation results, thus affecting the follow-up research to a certain extent; As a result, the scanner and the computer must coexist in this detection method, and both are indispensable. If there is only a scanner, although the biochip can be scanned, the scan result cannot be displayed, and if only the computer is used, the biochip cannot be scanned. , the same observation results cannot be obtained, and the scanner and the computer are relatively large in size, poor in portability, and inconvenient to use. At the same time, when reacting biological samples, nucleic acid amplification instruments, chip hybridization instruments, chip cleaning machines and other equipment need to be used. However, the above-mentioned complete sets of related instruments and equipment on the market are not only expensive, but also bulky and heavy, and are not suitable for frequent movement. , and difficult to move.

Contents of the invention

The technical problem to be solved by the utility model is to provide a qualitative/quantitative biochip detection system that is small in size, easy to move and capable of obtaining relatively accurate observation results.

The technical solution adopted by the utility model to solve the problems of the technologies described above is:

A qualitative/quantitative biochip detection device, comprising a detection box and a detection box, the detection box is provided with an image display mechanism, and the detection box is provided with a biochip reaction mechanism, a light source and an image capture mechanism, The image capturing mechanism is electrically connected with the image display mechanism.

The front end of the detection box is provided with the image display mechanism, the image display mechanism is a display screen, and the rear end of the detection box is provided with a plurality of A detection chamber, the detection box is detachably inserted into the detection chamber, the detection chamber is provided with an electrical connection interface, the electrical connection interface is electrically connected to the display screen, the The detection box is provided with an electrical connection plug matched with the electrical connection interface, and the image capturing mechanism is electrically connected with the electrical connection plug. The above structural design can effectively improve the reaction and detection efficiency of biological samples.

The biochip reaction mechanism includes a transparent reaction disc, and a plurality of reaction areas are arranged radially along the circumference of the reaction disc, and each reaction area includes liquid pools arranged sequentially from the inside to the outside. and the reaction pool, the liquid pool and the reaction pool are connected through a conduit, the center of the reaction disk is connected to the center of the liquid pool in each of the reaction areas, and the reaction pool The connecting line between the centers coincides with the central axis of the conduit, the reaction disc is provided with a plurality of sampling holes, and each of the liquid pools is connected with one of the sampling holes , the reaction disc is provided with a plurality of exhaust holes, and each side of the reaction pool is connected with one of the exhaust holes. Through the multiple reaction areas set on the reaction disc, the biochip reaction mechanism can carry out multiple sets of reactions at the same time, which improves the operating efficiency, and each reaction area includes liquid pools and reaction pools arranged sequentially from the inside to the outside , the liquid pool and the reaction pool are connected through a conduit, the liquid pool is provided with a sample hole, the biological sample and the reaction reagent are added into the liquid pool through the sample hole, and then a rotational force is applied to the reaction disc, the liquid pool Biological samples and reaction reagents can enter the reaction pool through the conduit under the action of the rotation force, and add other reagents in the process, the reagents are also added to the liquid pool through the sample hole, and then passed through the reaction circle The disc exerts a rotating force so that the reagent enters the reaction pool through the catheter. The structure is simple and the operation is convenient, which effectively prevents the biological sample from being contaminated during the reagent dropping process, thereby obtaining more accurate experimental data. Biological samples and reaction reagents can smoothly enter the reaction pool.

A check pool for preventing the liquid from flowing back into the liquid pool is provided between the liquid pool and the reaction pool, and the conduit is divided into a first conduit segment and a second conduit segment, and the liquid pool and the The check pools are connected through the first conduit section, the check pool and the reaction pool are connected through the second conduit section, and the center of the reaction disc The line between the center of the liquid pool, the center of the reaction pool and the center of the check pool in each of the reaction areas coincides with the central axis of the conduit, The width of the check pool is greater than the width of the conduit. The arrangement of the check tank with the above structure can effectively prevent the liquid in the reaction tank from flowing back into the liquid tank, thereby ensuring the normal progress of the reaction, with simple structure and stable use.

A reaction chamber is arranged in the detection box, a transparent chamber cover is reversibly arranged on the reaction chamber, a horizontal partition is arranged in the reaction chamber, and the horizontal partition divides the reaction The cavity is divided into an upper chamber and a lower chamber. A heating plate is arranged in the upper chamber, and a rest seat for placing the reaction disc is arranged on the horizontal partition. The image capture mechanism is A camera, the horizontal partition is provided with a shooting hole through up and down, the camera is facing the shooting hole for shooting the biological information on the reaction disc, the camera, the Both the heating plate and the light source are electrically connected to the electrical connection plug, and the light source is arranged on the inner end surface of the chamber cover for illuminating the upper chamber. The reaction chamber is used for the reaction of the reaction disk and the detection after the reaction is completed. The chamber cover set on the reaction chamber is a transparent chamber cover for easy observation. The horizontal partition divides the reaction chamber into an upper chamber and a lower chamber. The chamber is used for the reaction, and the lower chamber is used for the installation of the camera and other components, and the structure is neat; the camera is used to capture the image of the biological sample on the reaction disc after the reaction, and the heating plate is used to provide an environment for the reaction of the biological sample Temperature, the light source is used to provide illumination light for the camera to capture a clearer image of the biological sample.

The said rest seat is provided with a motor, and said motor is electrically connected with said electrical connection plug, and the output shaft of said motor extends vertically from said rest seat, and said output shaft is fixedly connected with a The installation column, the side of the installation column protrudes outwards and is provided with anti-rotation bumps, and the center of the reaction disc is coaxially provided with an installation hole cooperating with the installation column, and the installation An anti-rotation groove cooperating with the anti-rotation protrusion is arranged in the hole, and a shelving disc is coaxially arranged at the bottom of the installation column. The motor is used to provide centrifugal force to the reaction disc, and at the same time, the rotation of the motor drives the reaction disc to rotate during detection, and the camera can completely capture the information of all biological samples on a reaction disc; the anti-rotation bump and anti-rotation concave The cooperation of the groove ensures that the reaction disk can rotate synchronously with the output shaft of the motor, and the disk is placed to support the reaction disk.

A heat dissipation fan is arranged in the lower chamber, and the heat dissipation fan is electrically connected with the electrical connection plug, and a heat dissipation through slot is arranged at the bottom of the detection box. Through the setting of the heat dissipation fan and the cooperation of the heat dissipation channel, the temperature in the reaction chamber can drop rapidly after the reaction is completed, which protects the electrical components arranged therein.

A circuit board is arranged in the detection box, and a control circuit is arranged on the circuit board, and the display screen and the electrical connection interface are respectively electrically connected to the control circuit, and the detection box body A USB interface is also provided on the top, and the USB interface is electrically connected with the control circuit. The control circuit supplies power to the display screen; when the detection box is inserted into the detection box, the electrical connection interface and the corresponding inserted electrical connection socket realize electrical conduction, and the rotation of the motor, the heating of the heating plate and the shooting of the camera are controlled by the control circuit; in addition The USB interface can be used to transmit the picture data captured by the camera, and an external power supply can be connected to supply power to the control circuit.

A temperature sensor is arranged in the reaction chamber, the temperature measuring probe of the temperature sensor protrudes into the upper chamber, and the temperature sensor is electrically connected with the electrical connection plug. The temperature sensor is used to detect the temperature of the upper chamber, thereby driving the control circuit to control the heating plate to provide a more suitable reaction temperature.

A rechargeable battery is arranged in the detection box, and the rechargeable battery is electrically connected to the control circuit, and a charging interface for charging the rechargeable battery is arranged on the detection box, and the charging The interface is electrically connected with the rechargeable battery. The rechargeable battery is used to supply power to the control circuit, and the charging interface is used to charge the rechargeable battery, so that the rechargeable battery can better supply power to the entire device, making the entire device more portable.

Compared with the prior art, the utility model has the advantages that: the detection device integrates the reaction and detection of the biochip in a detection box, and after the reaction is completed, the light source is used to provide the biochip with a light source during detection, and then the biochip is captured by the image. The mechanism captures the image information on the biochip and transmits the captured image information to the image display mechanism. The structure is simple, the portability is good, and relatively accurate observation results can be obtained.

Description of drawings

Fig. 1 is the structural representation of reaction disk in the utility model;

Fig. 2 is the structural representation of removing the reaction disk from the detection box in the utility model;

Fig. 3 is the structural representation of removing biochip and chamber cover in detection box in the utility model;

Fig. 4 is the schematic cross-sectional structure diagram of the detection box installed with the reaction disk in the utility model;

Fig. 5 is a schematic diagram of the front three-dimensional structure of the detection box in the utility model;

Fig. 6 is a schematic diagram of the back stereoscopic structure of the detection box in the utility model;

Fig. 7 is a schematic cross-sectional structure diagram of one of the detection boxes inserted into the detection box of the present invention.

Detailed ways

The utility model is described in further detail below in conjunction with the accompanying drawings.

As shown in Figures 1 to 7, a qualitative/quantitative biochip detection device includes a detection box B and a detection box C, the detection box B is provided with an image display mechanism, and the detection box C is provided with a biochip reaction mechanism , a light source (not shown in the figure) and an image capture mechanism, the image capture mechanism is electrically connected to the image display mechanism.

In this specific embodiment, the front end of the detection box B is provided with an image display mechanism, the image display mechanism is a display screen B1, and the rear end of the detection box B is provided with a plurality of detection chambers B2 matched with the detection box C, The detection box C is detachably inserted into the detection chamber B2, and the detection chamber B2 is provided with an electrical connection interface B21, which is electrically connected to the display screen B1, and the detection box C is provided with a matching electrical connection interface B21. The electrical connection plug C1, the image capturing mechanism is electrically connected with the electrical connection plug C1.

In this specific embodiment, the biochip reaction mechanism includes a transparent reaction disc A1, and a plurality of reaction areas A2 are radially arranged in the reaction disc A1 along the circumference, and each reaction area A2 includes sequentially arranged from the inside to the outside. The liquid pool A21 and the reaction pool A22 are connected through a conduit between the liquid pool A21 and the reaction pool A22, and between the center of the reaction disk A1 and the center of the liquid pool A21 and the center of the reaction pool A22 in each reaction area A2 The connecting line coincides with the central axis of the conduit, the reaction disk A1 is provided with a plurality of sampling holes A11, each liquid pool A21 is connected with a sampling hole A11, and the reaction disk A1 is provided with a plurality of row Air hole A12, each side of the reaction pool A22 is communicated with an air vent A12.

In this specific embodiment, a check pool A23 for preventing liquid from flowing back into the liquid pool A21 is provided between the liquid pool A21 and the reaction pool A22, and the conduit is divided into a first conduit section A24 and a second conduit section A25, and the liquid pool A21 It communicates with the check pool A23 through the first conduit segment A24, and communicates with the check pool A23 and the reaction pool A22 through the second conduit segment A25. The center of the reaction disc A1 is connected to the liquid in each reaction area A2 The line between the center of pool A21, the center of reaction pool A22 and the center of check pool A23 coincides with the central axis of the conduit, and the width of check pool A23 is greater than the width of the conduit.

In this specific embodiment, a reaction chamber is arranged in the detection box C, and a transparent chamber cover C2 is reversibly arranged on the reaction chamber, and a horizontal partition C3 is arranged in the reaction chamber, and the horizontal partition C3 divides the reaction chamber into upper and lower parts. The chamber C4 and the lower chamber C5, the upper chamber C4 is provided with a heating plate C41, the horizontal partition C3 is provided with a shelf C31 for placing the reaction disc A1, the image capture mechanism is a camera C8, and the horizontal partition C3 There is a shooting hole C32 penetrating up and down. The camera C8 faces the shooting hole C32 and is used to shoot the biological information on the reaction disk A1. The camera C8, the heating plate C41 and the light source (not shown in the figure) are all electrically connected to the electrical connection plug C1 , the light source is arranged on the inner end surface of the chamber cover C2 for illuminating the upper chamber C4.

In this specific embodiment, a motor C6 is arranged inside the rest seat C31, and the motor C6 is electrically connected to the electrical connection plug C1. The output shaft C61 of the motor C6 extends vertically out of the rest seat C31, and a mounting column C62 is fixedly connected to the output shaft C61. , the side of the mounting column C62 protrudes outwards and is provided with an anti-rotation bump C621, and the center of the reaction disc A1 is coaxially provided with a mounting hole A13 that is matched with the mounting column C62. The block C621 matches the anti-rotation groove A131, and the bottom of the mounting column C62 is coaxially provided with a shelving disc C622.

In this specific embodiment, a cooling fan C51 is provided in the lower chamber C5, and the cooling fan C51 is electrically connected to the electrical connection plug C1, and the bottom of the detection box C is provided with a cooling channel C9.

In this specific embodiment, a circuit board B6 is arranged in the detection box B, and a control circuit is arranged on the circuit board B6. The display screen B1 and the electrical connection interface B21 are respectively electrically connected to the control circuit, and the detection box B is also provided with The USB interface B3 is electrically connected to the control circuit.

In this specific embodiment, a temperature sensor C7 is arranged in the reaction chamber, and the temperature measuring probe of the temperature sensor C7 extends into the upper chamber C4, and the temperature sensor C7 is electrically connected to the electrical connection plug C1.

In this specific embodiment, a rechargeable battery B4 is arranged in the detection box B, and the rechargeable battery B4 is electrically connected to the control circuit. The detection box B is provided with a charging interface B5 for charging the rechargeable battery B4, and the charging interface B5 is connected to the charging Battery B4 is electrically connected.

Claims (10)

1. A qualitative/quantitative biochip detection device, characterized in that it comprises a detection box and a detection box, the described detection box is provided with an image display mechanism, and the described detection box is provided with a biochip reaction mechanism, a light source and an image capture mechanism, the image capture mechanism is electrically connected to the image display mechanism. 2. A kind of qualitative/quantitative biochip detection device as claimed in claim 1, characterized in that the front end of the detection box is provided with the image display mechanism, and the image display mechanism is a display screen, The rear end of the detection box is provided with a plurality of detection cavities matched with the detection boxes, the detection boxes are detachably inserted into the detection cavities, and the detection cavities are set There is an electrical connection interface, the electrical connection interface is electrically connected to the display screen, the detection box is provided with an electrical connection plug that matches the electrical connection interface, and the image capture mechanism is connected to the The electrical connection plug described above is electrically connected. 3. a kind of qualitative/quantitative biochip detection device as claimed in claim 2, it is characterized in that described biochip reaction mechanism comprises a transparent reaction disk, radially arranged along the circumference in the described reaction disk There are a plurality of reaction areas, and each reaction area includes a liquid pool and a reaction pool arranged sequentially from the inside to the outside, and the liquid pool and the reaction pool are connected through a conduit, and the center of the reaction disc is connected to the The center of the liquid pool in each of the reaction areas and the line between the centers of the reaction pool coincide with the central axis of the conduit, and the reaction disk is provided with multiple A sample injection hole, each of the liquid pools is connected with one of the sample injection holes, and a plurality of exhaust holes are arranged on the reaction disc, and each of the side of the reaction pool is connected with each other. There is a said vent. 4. a kind of qualitative/quantitative biochip detection device as claimed in claim 3, it is characterized in that being provided with is used to prevent liquid from flowing back into the check pool of described liquid pool between described liquid pool and described reaction pool, The conduit is divided into a first conduit segment and a second conduit segment, the liquid pool communicates with the check pool through the first conduit segment, and the check pool communicates with the check pool The reaction pools are connected through the second conduit section, and the center of the reaction disk is connected to the center of the liquid pool in each of the reaction areas, the center of the reaction pool and the center of the reaction pool. The line between the centers of the check pool coincides with the central axis of the conduit, and the width of the check pool is larger than the width of the conduit. 5. a kind of qualitative/quantitative biochip detection device as claimed in claim 3 is characterized in that a reaction chamber is arranged in the described detection box, and a transparent chamber cover is reversibly arranged on the described reaction chamber, so A horizontal partition is arranged in the reaction chamber, and the horizontal partition divides the reaction chamber into an upper chamber and a lower chamber, and a heating plate is arranged in the upper chamber, and the horizontal partition A shelf for placing the reaction disk is provided on the top, the image capture mechanism is a camera, and a shooting hole penetrating up and down is arranged on the horizontal partition, and the camera is facing the shooting hole For photographing the biological information on the reaction disc, the camera, the heating plate and the light source are all electrically connected to the electrical connection plug, and the light source is arranged in the cavity The inner end face of the cover is used to illuminate said upper chamber. 6. A kind of qualitative/quantitative biochip detection device as claimed in claim 5, characterized in that a motor is arranged in the described rest seat, and the described motor is electrically connected with the described electrical connection plug, and the described motor The output shaft of the output shaft vertically protrudes from the rest seat, and a mounting post is fixedly connected to the output shaft. The center of the center is coaxially provided with a mounting hole cooperating with the mounting column, and an anti-rotation groove matching the anti-rotation bump is provided in the mounting hole, and the bottom of the mounting column is the same as The shaft is provided with a rest disc. 7. a kind of qualitative/quantitative biochip detection device as claimed in claim 5, it is characterized in that described lower chamber is provided with cooling fan, described cooling fan is electrically connected with described electrical connection plug, and described The bottom of the detection box is provided with a cooling channel. 8. A kind of qualitative/quantitative biochip detection device as claimed in claim 5, characterized in that a circuit board is arranged in the described detection box, and a control circuit is arranged on the described circuit board, and the described display screen and The electrical connection interfaces are respectively electrically connected to the control circuit, and the detection box is further provided with a USB interface, and the USB interface is electrically connected to the control circuit. 9. A qualitative/quantitative biochip detection device as claimed in claim 5, characterized in that a temperature sensor is arranged in the reaction chamber, and the temperature measuring probe of the temperature sensor extends into the upper In the chamber, the temperature sensor is electrically connected to the electrical connection plug. 10. A kind of qualitative/quantitative biochip detection device as claimed in claim 8, characterized in that a rechargeable battery is arranged in the detection box, and the rechargeable battery is electrically connected with the control circuit, and the The detection box is provided with a charging interface for charging the rechargeable battery, and the charging interface is electrically connected with the rechargeable battery.