CN209437410U - A tree shrew intestinal flora collection device - Google Patents

Abstract

The utility model provides a tree shrew intestinal flora collection device, comprising a tree shrew fixing device and a flora collection box, the flora collection box is arranged under the base of the tree shrew fixing device, and the base is provided with The matching track above the flora collection box forms a drawer structure, and the base is provided with a through hole consistent with the opening of the flora collection box. The sampling device is used to collect the sample first and then transfer it to the sampling tube. There is a filter membrane in the sampling device, and the urine can be discharged to reduce the pollution of the sample by urine; the intestinal flora sample can directly enter the sampling tube from the outlet tube, shortening the sample time. The collection time is shortened, and the microbial contamination of the sample by the environment during the sampling process is reduced. At the same time, it can save time and effort to fix the experimental animals, reduce the struggle of experimental animals, get rid of the problem of multi-person operation, improve sampling efficiency, prevent intestinal flora sample contamination, facilitate sample collection, the device can be reused, reduce sampling costs, and the operation is very convenient .

Description

A tree shrew intestinal flora collection device

technical field

The utility model relates to the technical field of collection of intestinal flora of animals, in particular to a device for collecting intestinal flora of tree shrews.

Background technique

Existing studies have shown that a decrease in the diversity of the gut microbiota is associated with obesity and other health problems, while an increase in the diversity of the gut microbiota is beneficial to metabolism and immunity. Gut flora has an important impact on human health, and has been reported to be closely related to cardiovascular and cerebrovascular diseases, obesity, diabetes, AIDS, autism, depression, and Alzheimer's disease. Therefore, the study of gut flora has become a research hotspot in recent years. The human intestinal flora is closely related to various diseases. When the dynamic balance between the intestinal flora and the human body and the external environment is broken, the intestinal flora will become imbalanced, which will cause diseases or aggravate the original diseases.

Experimental animal tree shrews are very similar to humans in terms of physiology, biochemical indicators and their regulatory mechanisms. In addition, they are more convenient to raise, observe and operate. Therefore, tree shrews can be used as animal models to replicate human diseases in the study of intestinal flora. . Therefore, in the study of intestinal flora, the collection of intestinal flora (also known as "fecal bacteria", "fecal bacteria") is very important.

At present, the intestinal flora of experimental animals is usually collected by massaging the abdomen or forcing the method and the combination of these two methods. The collection is collected by gently squeezing the abdomen of the experimental animal or pressing the tip of the tail end of the animal to cause the animal's stress response. Fecal bacteria at the end of an animal's rectum. However, in the operation, because the tree shrew is lively and active, at least two people are required to cooperate. One person holds the tree shrew to fix its head and upper limbs with one hand, and fixes its lower limbs with the other hand; the other person massages the abdomen or squeezes the tip of the tail end of the animal, and collects Fecal bacteria. One-person operation is basically impossible to complete.

On the other hand, at present, this sampling method usually uses a sterile cotton swab to collect the fecal bacteria of the experimental animals into the sampling tube. This method can quickly freeze the intestinal flora samples in a short period of time to ensure the integrity of the flora gene samples, but there are the following problems: on the one hand, the experimental subjects will continue to move during the sampling process and need to be fixed by a special person, resulting in inconvenient sampling On the other hand, due to the fact that the experimental subjects will continue to move and urinate during the sampling process, it is easy to cause cross-contamination of the collected samples by urine or operator glove attachments.

Utility model content

The utility model aims at the defects in the prior art, and its main purpose is to provide an intestinal flora collection device which can fix experimental tree shrews and prevent the samples from maintaining the integrity of the flora gene samples.

The technical scheme of the utility model is as follows:

A tree shrew intestinal flora collection device, comprising a tree shrew fixing device (1) and a flora collection box (2), the tree shrew fixing device (1) is provided with a support plate (12), a support support plate ( 12) the vertical support rod (17a) and the horizontal support rod (17b) matched with the vertical support rod; the described support plate (12) is provided with a chute (13), which is arranged on the top of the support plate (12) The head fixing part (11) is moved and adjusted on the chute (13) by a pulley, and is symmetrically arranged on both sides of the chute (13) for fixing tree shrew limbs fixing parts (14), tree shrew limbs fixing parts ( 14) is provided with a pulley and a chute (13) for adjustment; the pedestal (15) is arranged below the support plate through the chute (13); ), the base (15) is provided with a through hole (15a) consistent with the opening of the flora collection box (2).

Further, in order to facilitate the removal of animal feces, clear the collection box, and reuse it, the bacteria collection box (2) is a drawer type, consisting of a removable part (21) and a bacteria collection box fixing frame (22). Composition, the flora collection box fixed frame (22) is provided with through holes corresponding to the base through holes (15a), and the extractable part is separated into upper and lower two-layer box bodies by filter membranes (23).

Further, the angle between the base (15) and the support plate (12) is not less than 90 degrees.

Further, in order to facilitate the adjustment of the angle between the base and the support plate, the bottom of the support plate (12) is also provided with an adjustment plate (18) for adjusting the angle of the base and the support plate, and the base is provided with an adjustable base angle protrusions (19).

In order to prevent animals from being hurt, the head fixing part (11) is an arc-shaped groove, and the two ends of the arc-shaped groove are provided with buckles (11c) on the head fixing part, and the buckle is provided with a head Fastener Strap with Velcro (11a).

Preferably, elastic sponge or rubber (11b) for head protection is attached to the surfaces of the arc-shaped grooves.

In order to avoid injury to the limbs of the animal, the limb fixing part (14) is provided with an arc-shaped groove suitable for the limbs of the tree shrew, and the two ends of the arc-shaped groove are provided with buckles (14c) of the limb fixing part, The buckle (14c) of the limb fixing part is provided with a strap (14a) with Velcro on the limb fixing part, and elastic sponge or rubber (14b) on the limb fixing part is attached to the surface of the arc-shaped groove.

Further, the shape of the bacteria collection box (2) is cuboid, cube, cylinder or cone.

Working principle: When in use, install the bacteria collection box below through the track below the base, and then adjust the position of the head fixing device and limb fixing device on the support plate through the chute according to the size of the tree shrew, and fix the tree shrew well Make the tree shrew's tail just above the bottom, and then massage the tree shrew's abdomen to stimulate its intestinal peristalsis and make it defecate. Defecation directly enters the upper filter membrane of the flora collection box below through the through hole, and if there is urine, it passes through the filter membrane to the bottom of the flora collection box. When the collection is complete, take out the drawer-type flora collection box directly for experimentation.

Compared with the prior art, the intestinal flora collection device provided by the utility model has the following beneficial effects:

1) The utility model has a simple structure and is very convenient to operate. The experimental animal using the experimental animal fixing device can save time and effort to fix the experimental animal, get rid of the problem of multi-person operation, facilitate the operation of the experimenter, and reduce the animal's struggle during the experiment , its cost is low, and it has a good market promotion prospect.

2) It can improve sampling efficiency, prevent intestinal flora sample contamination, and facilitate sample collection. The drawer-type flora collection box is convenient and hygienic, easy to clean and disinfect, and reused. The sampling device is used to collect the sample first and then transfer it to the sampling tube. There is a filter membrane in the sampling device, and the urine can be discharged to reduce the pollution of the sample by the urine; the intestinal flora sample can directly enter the sampling tube from the outlet tube, shortening the sample time. The collection time is shortened, and the microbial contamination of the sample by the environment during the sampling process is reduced. The device can be reused to reduce sampling cost.

Description of drawings

Fig. 1 is the structural representation of the intestinal flora collection device in the preferred embodiment of the present invention;

Fig. 2 is a side view of the utility model

Figure 3 is a top view of the utility model

Fig. 4 is the schematic diagram of the utility model flora collection box

Reference signs: 1-tree shrew fixing device, 2-flora collection box, 11-head fixing part, 11a-bandage with Velcro on the head fixing part, 11b-head protection elastic sponge or rubber, 11c - snaps on the head mount, 12 - support plate, 13 - chute, 14 - limb mount, 14a - strap with velcro on the limb mount, 14b - elastic sponge on the limb mount or Rubber, 14c-snaps of limbs fixing parts, 15-base, 15a-through hole, 17a-vertical support rod, 17b-horizontal support rod, 18-adjustment plate for the angle of the support plate, 19-protrusion for adjusting the angle of the base, 21 - Extractable part, 22 - Bacteria collection box fixing frame, 23 - Filter membrane

Detailed ways

The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention.

A tree shrew intestinal flora collection device, comprising a tree shrew fixing device 1 and a flora collection box 2, the tree shrew fixing device 1 is provided with a support plate 12, a vertical support rod 17a supporting the support plate 12 and a The horizontal support bar 17b that the vertical support bar cooperates; The described support plate 12 is provided with a chute 13, and the head fixing part 11 that is arranged on the top of the support plate 12 moves and adjusts on the chute 13 by a pulley, and is symmetrically arranged on The chute 13 both sides are used to fix the tree shrew limbs fixing part 14, the tree shrew limbs fixing part 14 is provided with a pulley and the chute 13 is adjusted; the base 15 is arranged on the support plate below through the chute 13; The colony collection box 2 is arranged under the base 15 of the tree shrew fixing device 1 , and the base 15 is provided with a through hole 15 a consistent with the opening of the bacterial colony collection box 2 . In order to take out the animal feces conveniently, clean the collection box, and reuse it, the bacteria collection box 2 is a drawer type, consisting of a removable part 21 and a bacteria collection box fixing frame 22, and the bacteria collection box fixing frame 22 A through hole corresponding to the through hole 15a of the base is provided on the top, and the extractable part is composed of an upper and a lower two-layer box body separated by a filter membrane 23 . The angle between the base 15 and the support plate 12 is not less than 90 degrees. In order to facilitate the adjustment of the angle between the base and the support plate, the bottom of the support plate 12 is also provided with an adjustment plate 18 for adjusting the angle between the base and the support plate, and the base is provided with a protrusion 19 which can adjust the angle of the base. In order to prevent the animals from being hurt, the head fixing part 11 is an arc-shaped groove, and the two ends of the arc-shaped groove are provided with buckles 11c on the head fixing part, and the buckles 11c are provided with the upper part of the head fixing part. Strap 11a with Velcro. The surfaces of the arc-shaped grooves are all attached with head protection elastic sponge or rubber 11b. In order to avoid injury to the limbs of the animal, the limb fixing part 14 is provided with an arc-shaped groove suitable for the limbs of the tree shrew, and the two ends of the arc-shaped groove are provided with a buckle 14c of the limb fixing part, and on the buckle 14c There is a strap 14a with Velcro on the limb fixing part, and the elastic sponge or rubber 14b on the limb fixing part is attached to the surface of the arc groove. The shape of the flora collection box 2 is cuboid, cube, cylinder or cone.

When in use, install the lower flora collection box through the track below the base, and then adjust the position of the head fixing device and limb fixing device on the support plate through the chute according to the size of the tree shrew, and fix the tree shrew's head and limbs, and make the tree shrew's tail just above the bottom, then massage the tree shrew's abdomen to stimulate its intestinal peristalsis and make it defecate. Defecation directly enters the upper filter membrane of the flora collection box below through the through hole, and if there is urine, it passes through the filter membrane to the bottom of the flora collection box. When the collection is complete, take out the drawer-type flora collection box directly for experimentation.

Compared with the prior art, the intestinal flora collection device provided by the utility model has the following beneficial effects:

1) The utility model has a simple structure and is very convenient to operate. The experimental animal using the experimental animal fixing device can save time and effort to fix the experimental animal, get rid of the problem of multi-person operation, facilitate the operation of the experimenter, and reduce the animal's struggle during the experiment , its cost is low, and it has a good market promotion prospect.

2) It can improve sampling efficiency, prevent intestinal flora sample contamination, and facilitate sample collection. The drawer-type flora collection box is convenient and hygienic, easy to clean and disinfect, and reused. The sampling device is used to collect the sample first and then transfer it to the sampling tube. There is a filter membrane in the sampling device, and the urine can be discharged to reduce the pollution of the sample by the urine; the intestinal flora sample can directly enter the sampling tube from the outlet tube, shortening the sample time. The collection time is shortened, and the microbial contamination of the sample by the environment during the sampling process is reduced. The device can be reused to reduce sampling cost.

The above descriptions are preferred implementations of the present utility model, but should not be interpreted as limiting the patent scope of the present utility model. It should be pointed out that for those skilled in the art, some improvements and modifications can be made without departing from the principle of the utility model, and these improvements and modifications are also regarded as the protection scope of the utility model.

Claims (8)

1. A device for collecting tree shrew intestinal flora, characterized in that: it includes a tree shrew fixing device (1) and a flora collection box (2), and the tree shrew fixing device (1) is provided with a support plate (12 ), the vertical support bar (17a) supporting the support plate (12) and the horizontal support bar (17b) matched with the vertical support bar; the support plate (12) is provided with a chute (13), which is set on The head fixing part (11) on the top of the support plate (12) is adjusted by moving on the chute (13) through the pulley, and the fixing parts (14) for fixing the limbs of the tree shrew are arranged symmetrically on both sides of the chute (13). The limb fixing part (14) of the tree shrew is provided with a pulley and the chute (13) for adjustment; the pedestal (15) is set under the support plate through the chute (13); the bacterial collection box (2) is set on Below the base (15) of the tree shrew fixing device (1), the base (15) is provided with a through hole (15a) which is consistent with the opening of the bacteria collection box (2). 2. The tree shrew intestinal flora collection device according to claim 1, characterized in that: the flora collection box (2) is a drawer type, and the extractable part (21) and the flora collection box Composed of a fixed frame (22), the bacteria collection box fixed frame (22) is provided with a through hole corresponding to the through hole (15a) of the base, and is separated from the extractable part by a filter membrane (23) into upper and lower layers The box body is constituted, and the upper layer is open. 3. The tree shrew intestinal flora collection device according to claim 1, characterized in that: the angle between the base (15) and the support plate (12) is not less than 90 degrees. 4. The tree shrew intestinal flora collection device according to claim 1, characterized in that: the bottom of the support plate (12) is also provided with an adjustment plate (18) for adjusting the angle between the base and the support plate, and the The base is provided with a protrusion (19) that can adjust the angle of the base. 5. The tree shrew intestinal flora collection device according to claim 1, characterized in that: the head fixing part (11) is an arc-shaped groove, and the two ends of the arc-shaped groove are provided with head fixing The buckle (11c) on the piece is provided with a strap (11a) with Velcro on the head fixing part. 6. The tree shrew intestinal flora collection device according to claim 5, characterized in that: elastic sponge or rubber (11b) for head protection is attached to the surface of the arc-shaped groove. 7. The tree shrew intestinal flora collection device according to claim 1, characterized in that: said limb fixing part (14) is provided with an arc-shaped groove adapted to the limbs of the tree shrew, the arc-shaped The two ends of the groove are provided with buckles (14c) of the limbs fixing parts, and the buckles of the limbs fixing parts (14c) are provided with straps (14a) with Velcro on the limbs fixing parts, and the surfaces of the arc-shaped grooves are attached with limbs fixing parts Elastic sponge or rubber on part (14b). 8. The tree shrew intestinal flora collection device according to claim 2, characterized in that: the shape of the flora collection box (2) is cuboid, cube, cylinder or cone.