CN209260066U - A support device for array microfluidic spotting microfluidic control device - Google Patents

Abstract

The utility model discloses a supporting device for array microfluid spotting microfluidic control device, which comprises an I-shaped connecting plate frame, an inverted L-shaped fixed claw and an inverted L-shaped movable claw, and the I-shaped connecting plate frame One side of the lower end is fixedly connected to the L-shaped fixed claw, and the bottom is provided with a T-shaped chute, and a T-shaped slider is installed in the T-shaped chute, and the T-shaped slider is fixedly connected to the L-shaped movable claw, the L-shaped fixed claw and the L-shaped movable claw Through the screw screw connection, the L-shaped fixed claw and the L-shaped movable claw are connected with reverse threads, and the inner sides of the lower ends of the L-shaped fixed claw and the L-shaped movable claw are embedded in the grooves on both sides of the microfluidic control device. The lower side of the structure of the utility model is equipped with a clamping micro-flow control device, which is convenient to replace the wake control device of different templates, and realizes different liquid shape spotting. The structure of screw rod, fixed claw and movable claw is adopted, which is convenient for quick assembly and disassembly. The clamping is stable and reliable, and it is easy to adapt to microfluidic control devices of different sizes, which improves the utilization rate of the equipment and reduces the equipment cost.

Description

A support device for array microfluidic spotting microfluidic control device

technical field

The utility model relates to a support device for array microfluid spotting microfluidic control devices, which belongs to the technical field of microfluid spotting equipment.

Background technique

With the research and progress of the current biomedical industry, in order to meet the needs of single cell culture, micro-region DNA fluorescence detection, and biological cell sensing, people have to quickly and massively process arrays of various shapes on the surface of different substrate samples. There is a growing need. When adopting the microfluidic control device for array spotting for batch spotting, the existing technology does not have the fixation that can adapt to the microfluidic control devices of different sizes.

Contents of the invention

The technical problem to be solved by the utility model is: to provide a support device for array microfluid spotting microfluidic control device, so as to solve the above-mentioned problems in the prior art.

The technical solution adopted by the utility model is: a supporting device for array microfluidic spotting microfluidic control device, including an I-shaped connecting plate frame, an inverted L-shaped fixed claw and an inverted L-shaped movable claw, and the I-shaped One side of the lower end of the connecting plate frame is fixedly connected to the L-shaped fixed claw, and the bottom is provided with a T-shaped chute, and a T-shaped slider is installed in the T-shaped chute, and the T-shaped slider is fixedly connected to the L-shaped movable claw, and the L-shaped fixed claw and The L-shaped movable claw is connected by a screw screw, the threads of the L-shaped fixed claw and the L-shaped movable claw are connected to the screw, and the inner sides of the lower ends of the L-shaped fixed claw and the L-shaped movable claw are embedded in the grooves on both sides of the microfluidic control device. Inside.

Beneficial effects of the utility model: compared with the prior art, the structure of the utility model is equipped with a clamping micro-flow control device on the lower side, which is convenient for replacing the wake control device of different templates, and realizes different liquid shape spotting. The structure of screw, fixed claw and movable claw is convenient for quick assembly and disassembly, stable and reliable clamping, and easy to adapt to microfluidic control devices of different sizes, which greatly improves the utilization rate of equipment and reduces equipment cost.

Description of drawings

Fig. 1 is a schematic diagram of the overall structure;

Fig. 2 is a schematic diagram of the connection structure of the microfluidic control device;

Fig. 3 is a schematic structural diagram of a liquid sample sampling device;

Fig. 4 is a structural schematic diagram of a spotting template;

Fig. 5 is a schematic diagram of a support structure;

Fig. 6 is a schematic diagram of the screw nut driving mechanism in each direction of the XYZ three-axis moving platform.

Detailed ways

Below in conjunction with accompanying drawing and specific embodiment the utility model is further introduced.

Embodiment 1: As shown in Figures 1-6, a support device (bracket 6) for an array microfluidic spotting microfluidic control device, the support device (bracket 6) includes an I-shaped connecting plate frame 601, Inverted L-shaped fixed claw 602 and inverted L-shaped movable claw 607, one side of the lower end of the I-shaped connecting frame 601 is fixedly connected to the L-shaped fixed claw 602, and a T-shaped chute 603 is arranged at the bottom, and the T-shaped chute 603 is installed There is a T-shaped slider 604, the T-shaped slider 604 is fixedly connected to the L-shaped movable claw 607, the L-shaped fixed claw 602 and the L-shaped movable claw 607 are screwed through the screw rod 605, and the L-shaped fixed claw 602 is connected to the L-shaped movable claw 607 The threads at the screw rod 605 are reversed, and the inner sides of the lower ends of the L-shaped fixed claw 602 and the L-shaped movable claw 607 are embedded in the grooves 606 on both sides of the microfluidic control device 4 , and a handle 608 is connected to one end of the screw rod 605 .

Example 2: As shown in Figures 1-6, a high-throughput droplet array microfluidic spotting device includes an inverted T-shaped frame 1, an XYZ three-axis moving platform 2, a spotting plate 3 and a microfluidic control Device 4, an XYZ three-axis moving platform 2 is installed on the vertical plate of the T-shaped frame 1, and a point model 3 is installed on the XYZ three-axis moving platform 2, and a horizontal cantilever beam 5 is fixedly connected to the T-shaped frame 1, and the microfluidic The control device 4 is fixedly connected to the cantilever beam 5 through the bracket 6 and is located on the upper side of the spotting plate 3 , and the microfluidic control device 4 is connected with a liquid sample sampling device 7 .

Preferably, the above-mentioned microfluidic control device 4 includes a fixed plate 401 and a sample application template 402, the fixed plate 401 is detachably fixedly connected to the upper side of the sample application template 402, the upper end is provided with a sample injection microtube 403, and the sample application template 402 is provided with There is an array of spotting holes 404, the upper end of the array spotting holes 404 is provided with a groove 405 communicating with the upper end, the groove 405 is in communication with the sample injection microtube 403, the fixed plate 401 is provided with a threaded guide rod, and the threaded guide rod passes through the point The sample template is locked with nuts.

Preferably, the above-mentioned sample injection microtubes 403 are provided with multiple pieces, and the multiple pieces evenly divide the groove 405 on the upper side of the array spotting holes 404 .

Preferably, the above-mentioned groove 405 is communicated with a waste liquid micropipe 406 .

Preferably, the above-mentioned XYZ three-axis mobile platform 2 includes a Z-axis mobile platform 8, an X-axis mobile platform 9 and a Y-axis mobile platform 10 connected in sequence, and the Z-axis mobile platform 8, the X-axis mobile platform 9 and the Y-axis mobile platform 10 are all It includes a slider guide rail pair, a slide plate, and a lead screw nut pair connected with a platform drive motor. The slide plate of the Z-axis mobile platform 8 is connected to the vertical plate through the slider guide rail pair, and the bottom of the slide plate is connected with a vertical shaft that drives it to move. The screw nut pair, the slide plate of the X-axis moving table 9 is connected to the horizontal plate connected to the slide plate of the Z-axis moving table 8 through the slider guide rail pair, and the bottom of the slide plate is connected with a vertical screw nut pair that drives it to move. The slide plate of the Y-axis mobile platform 10 is connected to the slide plate of the X-axis mobile platform 9 through the slider guide rail pair, and the bottom of the slide plate is connected with a vertical screw nut pair that drives it to move.

Preferably, a heating plate 11 is provided between the XYZ three-axis moving platform 2 and the spotting plate 3 .

Preferably, a heat conduction plate 12 is provided between the heating plate 11 and the spotting plate 3 , and a temperature sensor 13 is provided between the heat conduction plate 12 and the heating plate 11 .

Preferably, the bracket 6 includes an I-shaped connecting frame 601, an inverted L-shaped fixed claw 602 and an inverted L-shaped movable claw 607. The lower end of the I-shaped connecting frame 601 is fixedly connected to the L-shaped fixed claw 602, and the bottom A T-shaped chute 603 is provided, and a T-shaped slider 604 is installed in the T-shaped chute 603. The T-shaped slider 604 is fixedly connected to the L-shaped movable claw 607, and the L-shaped fixed claw 602 and the L-shaped movable claw 607 pass through the screw rod 605. Spiral connection, L-shaped fixed claw 602 and L-shaped movable claw 607 are connected to screw rod 605 where the thread is reversed, and the lower ends of the L-shaped fixed claw 602 and L-shaped movable claw 607 are embedded in the grooves 606 on both sides of the microfluidic control device 4 Inside, a handle 608 is connected to one end of the screw rod 605 .

Preferably, the above-mentioned liquid sample sampling device 7 includes a flow equalization valve 701, a microcavity syringe 702 and a sample syringe 703, the liquid outlet of the microcavity syringe 702 is connected to the liquid inlet of the flow equalization valve 701 through a pipeline, and the inlet on the upper side The liquid port is connected with a sample injector 703, and the equalizing valve 701 and the microcavity injector 702 are fixedly connected to the top of the cantilever beam 5. The equalizing valve 701 is provided with a liquid chamber communicating with the liquid inlet, and the liquid chamber is provided with a plurality of liquid outlets , multiple liquid outlets are connected to multiple sampling microtubes 403 .

Preferably, the above-mentioned microcavity syringe 702 includes a microcavity 704, a piston 705 and a piston rod 706, the piston 705 is placed in the microcavity 704 in a sealed manner, one side is connected with a piston rod 706, and the piston rod 706 passes through the microcavity 704 in a sealed connection. Connected to the output shaft of the sample injection drive motor 707 , the sample injection drive motor 707 is connected to the microcavity 704 through a linear bearing 708 .

The advantages of the above-mentioned high-throughput droplet array microfluidic spotting device are as follows:

(1) Through the microfluidic control device and the three-axis moving platform, the device can quickly realize the spotting of tiny liquids on the spotting plate, and the spotting is fast and accurate. Different microfluidic control devices are used to facilitate spotting into different spotting shapes , to adapt to different needs, easy to control flow and speed, save time and effort in operation, the device also has the characteristics of simple structure, low cost, convenient and quick operation;

(2) The detachable structure of the microfluidic control device adopted by the device is convenient for replacing the spotting plates of different templates, and the grooves are used to connect multiple array spotting holes, which can quickly realize the spotting of samples and greatly improve the spotting efficiency;

(3) Multiple sampling microtubes are installed, which can simultaneously fill multiple grooves connected to the array sample hole, improve the liquid filling efficiency, reduce the flow time of the liquid in the groove, and improve the sampling efficiency;

(4) The waste liquid microtube plays the role of recovering the waste liquid on the one hand, and on the other hand plays the role of exhaust, improving the fluidity of the sample liquid;

(5) Through the three-way movement of the connected Z-axis mobile table, X-axis mobile table and Y-axis mobile table, the position adjustment between the spotting sample plate and the microfluidic control device can be quickly realized, the adjustment is fast and accurate, and the spotting efficiency is improved;

(6) Set up a heating plate to facilitate the realization of spotting samples at a constant temperature and avoid the influence of temperature changes on spotting;

(7) Set up a heat conduction plate to achieve uniform heat transfer and avoid unevenness caused by excessive local temperature. Set up a temperature sensor to monitor the temperature of the heating plate in real time, which is convenient for controlling the heating temperature of the sample;

(8) The support structure is set, and the clamping micro-flow control device is set on the lower side, which is convenient for replacing the wake control device of different templates, and realizes different liquid shape spotting. The structure of screw, fixed claw and movable claw is adopted, which is convenient for quick installation. Disassembly, clamping is stable and reliable, and it is also easy to adapt to microfluidic control devices of different sizes, greatly improving the utilization rate of equipment and reducing equipment cost;

(9) The liquid sample sampling device adopts flow equalizing valve, microcavity syringe and sample syringe, which can realize rapid injection and rapid addition of sampled liquid samples;

(10) The piston rod is driven by the sample injection drive motor, and moves back and forth on the linear bearing to realize the rapid addition of sample liquid.

The above is only a specific embodiment of the present utility model, but the scope of protection of the present utility model is not limited thereto. Anyone familiar with the technical field can easily think of changes or changes within the technical scope disclosed by the utility model Replacement should be covered within the protection scope of the present utility model, therefore, the protection scope of the present utility model should be based on the protection scope of the claims.

Claims (1)

1. a kind of support device for array microfluidic point sample microfluidic control device, it is characterised in that: including I-shaped connection Grillage (601), the L-type fixed jaw (602) to stand upside down and the L-type movable claw (607) to stand upside down, I-shaped connection grillage (601) lower end Side is fixedly connected L-type fixed jaw (602), and bottom is provided with T-type sliding slot (603), is equipped with T-type sliding block in T-type sliding slot (603) (604), T-type sliding block (604) is fixedly connected L-type movable claw (607), and L-type fixed jaw (602) and L-type movable claw (607) pass through silk Bar (605) spiral connects, and the screw thread at L-type fixed jaw (602) and L-type movable claw (607) connection wire rod (605) is reversed, and L-type is solid Determine pawl (602) and L-type movable claw (607) lower end inside is embedded into the groove (606) of microfluidic control device (4) two sides.