CN109439514B - Supporting device for array micro-fluid sample application micro-flow control device - Google Patents
Supporting device for array micro-fluid sample application micro-flow control device Download PDFInfo
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- CN109439514B CN109439514B CN201811536258.8A CN201811536258A CN109439514B CN 109439514 B CN109439514 B CN 109439514B CN 201811536258 A CN201811536258 A CN 201811536258A CN 109439514 B CN109439514 B CN 109439514B
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- 239000012530 fluid Substances 0.000 title abstract description 7
- 210000000078 claw Anatomy 0.000 claims abstract description 17
- 239000007788 liquid Substances 0.000 abstract description 25
- 238000002347 injection Methods 0.000 description 10
- 239000007924 injection Substances 0.000 description 10
- 238000010438 heat treatment Methods 0.000 description 5
- 239000002699 waste material Substances 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000003491 array Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000001917 fluorescence detection Methods 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000002791 soaking Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/16—Microfluidic devices; Capillary tubes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/48—Holding appliances; Racks; Supports
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Clinical Laboratory Science (AREA)
- Dispersion Chemistry (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a supporting device for an array micro-fluid sample application micro-flow control device, which comprises an I-shaped connecting plate frame, an inverted L-shaped fixed jaw and an inverted L-shaped movable jaw, wherein one side of the lower end of the I-shaped connecting plate frame is fixedly connected with the L-shaped fixed jaw, a T-shaped sliding groove is arranged at the bottom of the I-shaped connecting plate frame, a T-shaped sliding block is arranged in the T-shaped sliding groove and is fixedly connected with the L-shaped movable jaw, the L-shaped fixed jaw and the L-shaped movable jaw are in spiral connection through a screw rod, threads at the connecting screw rod of the L-shaped fixed jaw and the L-shaped movable jaw are reversed, and the inner sides of the lower ends of the L-shaped fixed jaw and the L-shaped movable jaw are embedded into grooves at two sides of the micro-flow control device. The clamping micro-flow control device is arranged at the lower side in the structure of the invention, so that the wake flow control devices of different templates can be conveniently replaced, different liquid shape sample application can be realized, the screw, the fixed claw and the movable claw are adopted, the quick assembly and disassembly are convenient, the clamping is stable and reliable, the micro-flow control device with different sizes can be conveniently adapted, the utilization rate of equipment is improved, and the equipment cost is reduced.
Description
Technical Field
The invention relates to a supporting device for an array micro-fluid sample application micro-flow control device, and belongs to the technical field of micro-fluid sample application equipment.
Background
With the research and progress of the current biomedical industry, in order to meet the requirements of single cell culture, micro-region DNA fluorescence detection, biological cell sensing and the like, there is an increasing need for rapid and massive processing of arrays with various shapes on the surfaces of different substrate samples. When mass spotting is performed using array spotted microflow control devices, the prior art does not have a fixture that can accommodate microflow control devices of different sizes.
Disclosure of Invention
The invention aims to solve the technical problems that: a support device for an array microfluidic spotting micro-flow control device is provided to solve the above-mentioned problems of the prior art.
The technical scheme adopted by the invention is as follows: a supporting device for an array micro-fluid sample application micro-flow control device comprises an I-shaped connecting plate frame, an inverted L-shaped fixing jaw and an inverted L-shaped movable jaw, wherein one side of the lower end of the I-shaped connecting plate frame is fixedly connected with the L-shaped fixing jaw, a T-shaped sliding groove is formed in the bottom of the I-shaped connecting plate frame, a T-shaped sliding block is arranged in the T-shaped sliding groove and fixedly connected with the L-shaped movable jaw, the L-shaped fixing jaw and the L-shaped movable jaw are in threaded connection through a screw rod, threads at the connecting screw rod of the L-shaped fixing jaw and the L-shaped movable jaw are reversed, and the inner sides of the lower ends of the L-shaped fixing jaw and the L-shaped movable jaw are embedded into grooves on two sides of the micro-flow control device.
The invention has the beneficial effects that: compared with the prior art, the structure of the invention has the advantages that the clamping micro-flow control device is arranged at the lower side, the wake flow control devices of different templates are convenient to replace, different liquid shape sample application is realized, the screw rod, the fixed claw and the movable claw are adopted, the quick assembly and the disassembly are convenient, the clamping is stable and reliable, the micro-flow control devices of different sizes are also convenient to adapt, the utilization rate of equipment is greatly improved, and the equipment cost is reduced.
Drawings
FIG. 1 is a schematic overall construction;
FIG. 2 is a schematic diagram of a microflow control device connection structure;
FIG. 3 is a schematic diagram of a liquid sample injection device;
FIG. 4 is a schematic view of the structure of a spotting template;
FIG. 5 is a schematic view of a stent structure;
fig. 6 is a schematic view of a drive mechanism for a screw in each direction of an XYZ three-axis moving platform.
Detailed Description
The invention will be further described with reference to the accompanying drawings and specific examples.
Example 1: as shown in fig. 1-6, a support device (support 6) for an array micro-fluidic sample application micro-flow control device, the support device (support 6) comprises an i-shaped connecting plate frame 601, an inverted L-shaped fixing jaw 602 and an inverted L-shaped movable jaw 607, wherein one side of the lower end of the i-shaped connecting plate frame 601 is fixedly connected with the L-shaped fixing jaw 602, a T-shaped sliding groove 603 is arranged at the bottom, a T-shaped sliding block 604 is arranged in the T-shaped sliding groove 603, the T-shaped sliding block 604 is fixedly connected with the L-shaped movable jaw 607, the L-shaped fixing jaw 602 and the L-shaped movable jaw 607 are in spiral connection through a screw rod 605, the threads of the connection screw rod 605 of the L-shaped fixing jaw 602 and the L-shaped movable jaw 607 are reversed, the inner sides of the lower ends of the L-shaped fixing jaw 602 and the L-shaped movable jaw 607 are embedded into grooves 606 on two sides of the micro-flow control device 4, and one end of the screw rod 605 is connected with a handle 608.
Example 2: as shown in fig. 1-6, a high-throughput droplet array micro-fluid sample application device comprises an inverted T-shaped frame 1, an XYZ three-axis moving platform 2, a sample plate 3 and a micro-flow control device 4, wherein the XYZ three-axis moving platform 2 is arranged on a vertical plate of the T-shaped frame 1, the sample plate 3 is arranged on the XYZ three-axis moving platform 2, the T-shaped frame 1 is fixedly connected with a horizontal cantilever beam 5, the micro-flow control device 4 is fixedly connected with the cantilever beam 5 through a bracket 6 and is positioned on the upper side of the sample plate 3, and the micro-flow control device 4 is connected with a liquid sample injection device 7.
Preferably, the micro-flow control device 4 includes a fixing plate 401 and a sample application template 402, the fixing plate 401 is detachably and fixedly connected to the upper side of the sample application template 402, the upper end of the fixing plate is provided with a sample application microtube 403, the sample application template 402 is provided with an array sample application hole 404, the upper end of the array sample application hole 404 is provided with a groove 405 communicated with the upper end of the array sample application hole, the groove 405 is communicated with the sample application microtube 403, the fixing plate 401 is provided with a threaded guide rod, and the threaded guide rod is locked by a nut after passing through the sample application template.
Preferably, the sample injection microtubes 403 are provided with a plurality of grooves 405 on the upper side of the array sample application holes 404.
Preferably, the groove 405 is connected to a waste liquid microtube 406.
Preferably, the XYZ three-axis moving platform 2 includes a Z-axis moving platform 8, an X-axis moving platform 9 and a Y-axis moving platform 10 which are sequentially connected, wherein each of the Z-axis moving platform 8, the X-axis moving platform 9 and the Y-axis moving platform 10 includes a slider guide rail pair, a slide plate, and a screw nut pair connected with a platform driving motor, the slide plate of the Z-axis moving platform 8 is connected to a vertical plate through the slider guide rail pair, the slide plate bottom of the slide plate is connected to a vertical screw nut pair driving the slide plate to move, the slide plate of the X-axis moving platform 9 is connected to a horizontal plate connected with the slide plate of the Z-axis moving platform 8 through the slider guide rail pair, the slide plate bottom of the Y-axis moving platform 10 is connected to the slide plate of the X-axis moving platform 9 through the slider guide rail pair, and the slide plate bottom is connected to a vertical screw nut pair driving the slide plate to move.
Preferably, a heating plate 11 is provided between the XYZ three-axis moving stage 2 and the spot plate 3.
Preferably, a heat conducting plate 12 is provided between the heating plate 11 and the spot plate 3, and a temperature sensor 13 is provided between the heat conducting plate 12 and the heating plate 11.
Preferably, the bracket 6 includes an i-shaped connecting plate frame 601, an inverted L-shaped fixing jaw 602 and an inverted L-shaped movable jaw 607, wherein the L-shaped fixing jaw 602 is fixedly connected to one side of the lower end of the i-shaped connecting plate frame 601, a T-shaped sliding groove 603 is arranged at the bottom of the i-shaped connecting plate frame, a T-shaped sliding block 604 is installed in the T-shaped sliding groove 603, the T-shaped sliding block 604 is fixedly connected with the L-shaped movable jaw 607, the L-shaped fixing jaw 602 and the L-shaped movable jaw 607 are in spiral connection with each other through a screw rod 605, threads at the positions where the L-shaped fixing jaw 602 and the L-shaped movable jaw 607 are connected with the screw rod 605 are reversed, the inner sides of the lower ends of the L-shaped fixing jaw 602 and the L-shaped movable jaw 607 are embedded into grooves 606 at two sides of the micro-flow control device 4, and one end of the screw rod 605 is connected with a handle 608.
Preferably, the liquid sample injection device 7 includes a flow equalizing valve 701, a microcavity injector 702 and a sample injector 703, wherein a liquid outlet of the microcavity injector 702 is connected to a liquid inlet of the flow equalizing valve 701 through a pipeline, the liquid inlet on the upper side is connected with the sample injector 703, the flow equalizing valve 701 and the microcavity injector 702 are fixedly connected to the top of the cantilever beam 5, a liquid cavity communicated with the liquid inlet is arranged in the flow equalizing valve 701, a plurality of liquid outlets are arranged in the liquid cavity, and a plurality of liquid outlets are connected with the sample injection microtubes 403.
Preferably, the microcavity injector 702 comprises a microcavity 704, a piston 705 and a piston rod 706, wherein the piston 705 is hermetically arranged in the microcavity 704, one side of the piston 705 is connected with the piston rod 706, the piston rod 706 penetrates out of the microcavity 704 in a hermetically connected manner and is connected to an output shaft of a sample injection driving motor 707, and the sample injection driving motor 707 is connected to the microcavity 704 through a linear bearing 708.
The high-throughput droplet array microfluidic spotting device has the following advantages:
(1) The device can rapidly realize the sample application of tiny liquid on a sample plate through the micro-flow control device and the triaxial mobile platform, the sample application is rapid and accurate, different sample application shapes are conveniently formed by adopting different micro-flow control devices, different requirements are met, the flow and speed are conveniently controlled, the operation is time-saving and labor-saving, and the device also has the characteristics of simple structure, low cost and convenient and fast operation;
(2) The detachable structure of the micro-flow control device adopted by the device is convenient for replacing sample application plates of different templates, and the grooves are adopted to communicate a plurality of array sample application holes, so that sample application of samples can be realized rapidly, and sample application efficiency is improved greatly;
(3) The plurality of sample injection microtubes are arranged, so that liquid can be filled into a plurality of grooves communicated with the array sample application holes at the same time, the liquid filling efficiency is improved, the flowing time of liquid in the grooves is reduced, and the sample application efficiency is improved;
(4) The waste liquid microtube has the function of recycling waste liquid on one hand, and has the function of exhausting on the other hand, so that the fluidity of sample application liquid is improved;
(5) The position between the spotting plate and the micro-flow control device can be quickly adjusted by three-way movement of the Z-axis moving table, the X-axis moving table and the Y-axis moving table which are connected, the adjustment is quick and accurate, and the spotting efficiency is improved;
(6) The heating plate is arranged, so that constant-temperature sample application of the sample plate is realized, and the influence of temperature change on sample application is avoided;
(7) The heat-conducting plate is arranged, so that soaking transmission can be realized, non-uniformity caused by overhigh local temperature is avoided, and the temperature sensor is arranged, so that the temperature of the heating plate can be monitored in real time, and the heated temperature of the spot plate can be controlled conveniently;
(8) The lower side of the bracket structure is provided with the clamping micro-flow control device, so that the wake flow control devices of different templates can be replaced conveniently, different liquid shapes can be spotted, the screw, the fixed claw and the movable claw are adopted, the quick assembly and disassembly are convenient, the clamping is stable and reliable, the micro-flow control devices of different sizes can be adapted conveniently, the utilization rate of equipment is greatly improved, and the equipment cost is reduced;
(9) The liquid sample injection device adopting the flow equalizing valve, the microcavity injector and the sample injector can realize the rapid injection and rapid addition of the spotted liquid sample;
(10) The sample feeding driving motor is adopted to drive the piston rod to move, and the piston rod moves back and forth on the linear bearing, so that the sample application liquid is rapidly added.
The foregoing is merely illustrative of the present invention, and the scope of the present invention is not limited thereto, and any person skilled in the art can easily think about variations or substitutions within the scope of the present invention, and therefore, the scope of the present invention shall be defined by the scope of the appended claims.
Claims (1)
1. A support device for an array microfluidic spotting microflow control device, characterized in that: the novel inverted T-shaped movable claw comprises an I-shaped connecting plate frame (601), an inverted L-shaped fixing claw (602) and an inverted L-shaped movable claw (607), wherein the L-shaped fixing claw (602) is fixedly connected to one side of the lower end of the I-shaped connecting plate frame (601), a T-shaped sliding groove (603) is formed in the bottom of the I-shaped connecting plate frame, a T-shaped sliding block (604) is arranged in the T-shaped sliding groove (603), the T-shaped sliding block (604) is fixedly connected with the L-shaped movable claw (607), the L-shaped fixing claw (602) and the L-shaped movable claw (607) are in threaded connection through a screw rod (605), threads at the position, where the L-shaped fixing claw (602) and the L-shaped movable claw (607) are connected are reversed, the inner sides of the lower ends of the L-shaped fixing claw (602) and the L-shaped movable claw (607) are embedded into grooves (606) at two sides of a micro-flow control device (4), the I-shaped connecting plate frame (601) is fixedly connected to a cantilever beam (5), and the cantilever beam (5) is horizontally and fixedly connected to the top of the T-shaped frame (1).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811536258.8A CN109439514B (en) | 2018-12-14 | 2018-12-14 | Supporting device for array micro-fluid sample application micro-flow control device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811536258.8A CN109439514B (en) | 2018-12-14 | 2018-12-14 | Supporting device for array micro-fluid sample application micro-flow control device |
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| Publication Number | Publication Date |
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| CN109439514A CN109439514A (en) | 2019-03-08 |
| CN109439514B true CN109439514B (en) | 2024-08-06 |
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| CN113996356A (en) * | 2021-10-27 | 2022-02-01 | 北京京东方技术开发有限公司 | Micro-fluidic chip, manufacturing method thereof and medical detection equipment |
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