CN207810687U - A kind of automatic nematode device for sorting - Google Patents
A kind of automatic nematode device for sorting Download PDFInfo
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- CN207810687U CN207810687U CN201721831498.1U CN201721831498U CN207810687U CN 207810687 U CN207810687 U CN 207810687U CN 201721831498 U CN201721831498 U CN 201721831498U CN 207810687 U CN207810687 U CN 207810687U
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- needle
- nematode
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- needle holder
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Abstract
The utility model discloses a kind of automatic nematode device for sorting, it is related to biological study apparatus field, including vertical bracing strut, microexamination camera lens, needle holder, chooses worm needle and motion controller；The microexamination camera lens is connect with the top of vertical bracing strut；The syringe needle for choosing worm needle includes supporting part, chooses worm needle and is arranged on needle holder；Needle holder is arranged close to the central axes of microexamination camera lens so that the edge of supporting part is overlapped with central axes；First motor is provided on needle holder, for driving moving up and down for needle holder, first motor is electrically connected first motor with motion controller, and the motion controller is used to control direction and the distance of needle holder movement.The utility model has the advantages that choosing worm automates, is accurate and convenient.
Description
Technical field
The utility model is related to biological study apparatus fields, more particularly to a kind of automatic nematode device for sorting.
Background technology
Caenorhabditis elegan adult polypide length is about 1.5 millimeters, and whole body is transparent under the microscope, is research mankind's senile dementia
The important model animal of disease, parkinsonism etc. neurogenic disease.It needs to carry out picking to nematode in the course of the research, that is, needs
It is moved to another culture dish from a culture dish.Operating personnel are needed to observe under the microscope and manual in the process of moving
With worm needle is chosen by polypide careful picking to another plate, this process is more fine and laborious.And manually choose worm
Environment opens, and is easy so that the adventives such as plate contaminated mold and acarid, interference experiment result.
In artificial picking nematode, nematode can generally be chosen the top of worm needle dial, and nematode is moved to mesh from top
Tablet on when, need to allow nematode being moved on target culture dish slowly, this action relatively expends the time, another disadvantage is
It needs to choose worm with larger plate, because of the inconvenient picking nematode in the smaller plate of diameter.
Utility model content
For this reason, it may be necessary to provide a kind of automatic nematode device for sorting, worm inefficiency and working environment are manually chosen for solving
The problem of being easily contaminated.
To achieve the above object, a kind of automatic nematode device for sorting, including vertical bracing strut, micro- sight are inventor provided
It examines camera lens, needle holder, choose worm needle and motion controller；The microexamination camera lens is connect with the top of vertical bracing strut；It is described
The syringe needle for choosing worm needle includes supporting part, chooses worm needle and is arranged on needle holder；Needle holder is set close to the central axes of microexamination camera lens
It sets so that the edge of supporting part is overlapped with central axes；First motor is provided on needle holder, first motor is for driving needle holder
Move up and down, first motor is electrically connected with motion controller, the motion controller be used for control needle holder movement direction
And distance.
Further, further include that two dimension automatically moves objective table, the two dimension automatically moves objective table and is arranged in vertical axis
The bottom end of holder.
Further, the two dimension automatically moves and is provided with the second motor on objective table, and the second motor is for driving two dimension
Automatically move objective table movement planar, the second motor be electrically connected with the controller, controller for control two dimension from
The direction of dynamic moving stage movement and distance.
Further, the two dimension, which automatically moves, is provided at least two fixing grooves on objective table.
Further, the two dimension automatically moves on objective table setting there are two LED light, two LED light be respectively embedded into
In two fixing grooves.
Further, the two dimension automatically moves and is provided at least one laser light source on objective table, the microexamination
Optical filter is provided on camera lens.
Further, electric heater is provided in the needle holder, the electric heater chooses the heating of worm needle for giving.
Further, the supporting part is grafter shape supporting part.
Further, the angle of plane is where the central axes of grafter shape supporting part automatically move objective table with two dimension
30 degree to 60 degree.
Further, further include controlling screen, the control screen is electrically connected with motion controller and microexamination camera lens,
It is provided with automatical worm button on control screen and prepares to choose worm button.
Be different from the prior art, in above-mentioned technical proposal, vertical bracing strut for fix microexamination camera lens, needle holder and
Motion controller；Microexamination camera lens is for observing the location of nematode so that in manual calibration, in microexamination camera lens
The quasi- heart of cross can be accurately aligned with the side of nematode；Needle holder chooses worm needle for fixation, chooses worm needle and is used for picking nematode,
The supporting part of syringe needle is used for support nematode；The first motor being arranged on needle holder is used to drive the lower upper movement of needle holder, movement
Controller is for controlling the direction and distance that needle holder moves up and down so that needle holder can be automatically moved, thus steadily certainly
Dynamic ground is triggered and put down by nematode.
Description of the drawings
Fig. 1 is the structural schematic diagram of automatic nematode device for sorting in one specific embodiment of the utility model；
Fig. 2 is the structural schematic diagram of automatic nematode device for sorting in the utility model another specific embodiment.
Reference sign：
1, vertical bracing strut；
2, microexamination camera lens；20, the central axes of microexamination camera lens；
3, needle holder；
4, first motor；
5, worm needle is chosen；
50, grafter shape supporting part；
6, motion controller；
7, two dimension automatically moves objective table；
70, fixing groove；
71, LED light；
72, laser light source；
8, the second motor；
9, screen is controlled.
Specific implementation mode
For the technology contents of technical solution, construction feature, the objects and the effects are described in detail, below in conjunction with specific reality
It applies example and attached drawing is coordinated to be explained in detail.
Referring to Fig. 1, the present embodiment provides a kind of automatic nematode device for sorting, it is used for nematode from source tablet picking to mesh
It marks on tablet, the target plate and source tablet can be 96 orifice plates, 24 orifice plates, 96 hole PCR plates, PCR8 townhouse pipes, the device
The take-up worm by the way of automation, more high efficiency.Automatic nematode device for sorting includes vertical bracing strut 1, microexamination mirror
First 2, needle holder 3, choose worm needle 5 and motion controller 6.Vertical bracing strut is Contraband shape holder, Contraband shape holder include first crossbar or
Transverse slat, second crossbar or transverse slat and montant or riser, first crossbar or transverse slat and second crossbar or transverse slat respectively with montant or perpendicular
The both ends vertical connection of plate.First crossbar or transverse slat are for fixing microexamination camera lens, second crossbar or transverse slat for placing source
Tablet and target plate, or automatically move objective table, the camera lens ontology and vertical axis of microexamination camera lens for placing two dimension
The second crossbar or transverse slat of holder are oppositely disposed.
Worm needle 5 is chosen for picking nematode, in order to smoothly provoke nematode from the tablet of source, it includes needle handle and needle to choose worm needle
Head, syringe needle include supporting part, and in picking nematode, nematode support from the bottom to top is lived in supporting part.In a certain embodiment, hold
Support portion is grafter shape supporting part 50, and supporting part favours the needle handle setting for choosing worm needle.Nematode is by supporting part support in order to prevent
It is slid after rising, it is preferable that the angle of plane is 30 degree where the central axes of grafter shape supporting part automatically move objective table with two dimension
To 60 degree.Such setting enables nematode to be risen by supporting part support, and will not be slid from supporting part.
In the particular embodiment, the needle holder is arranged in first motor 4, and first motor 4 is arranged in microexamination
On the side wall of camera lens 2, first motor 4 is electrically connected with motion controller 6.First motor is for driving needle holder along microexamination
The side wall of camera lens moves up and down, and motion controller is used to control displacement distance and the direction of first motor, such indirect control
The displacement distance of needle holder and direction, the final control realized to choosing worm needle displacement distance and direction.Needle holder is chosen for being clamped
Worm needle chooses worm needle and is coaxially set with needle holder, and for the ease of replacing needle holder, the needle holder can be removably disposed in
It chooses in worm needle, specifically, can be arranged for inserting the cavity for choosing worm needle in needle holder.
In the particular embodiment, in order to contraposition, needle holder can be facilitated to be positioned close to the axis of microexamination camera lens 2
At line 20 so that the edge of supporting part is overlapped with the central axes 20 of microexamination camera lens.
In a preferred embodiment, electric heater can be set in the needle holder, and electric heater is used for used
Worm needle heat sterilization is chosen, such setting can prevent the cross contamination of bacterium or nematode.Electric heater can be it is self-powered,
It can also be external power supply.
The source tablet and target plate can be placed at second crossbar or the transverse slat of vertical bracing strut, can also hung down
Two dimension is set at the second crossbar or transverse slat of d-axis holder and automatically moves objective table 7.Specifically, two dimension automatically moves objective table 7
It is arranged on the second motor 8, the second motor is arranged at the second crossbar of vertical bracing strut or transverse slat, and the second motor is controlled with movement
Device electrical connection processed.Motion controller is for controlling the second motor in the direction that the second motor planar moves and distance, and the
Two motors are used to drive two dimension to automatically move objective table and automatically move the movement planar of objective table institute in two dimension, and two dimension is automatically
Moving stage is for placing source tablet and target plate.In the bottom end of vertical bracing strut, the second motor and the automatic shifting of two dimension are set
Dynamic object stage, meanwhile, source tablet and target plate are adjacent to and is placed on two dimension and automatically moves on objective table so that source tablet and
Target plate can move under the drive that two dimension automatically moves objective table.Calibrating operation is removed, during choosing worm operation,
User only need by motion controller pre-set two dimension automatically move the direction of motion and distance of objective table, movement
Controller can control the second motor according to preset data and repeat to move, and the second motor controlled drives two dimension is automatic to move
Automatically moving for moving platform movement, final realization source tablet and target plate, is not necessarily to the intervention of operating personnel, realizes automatically in this way
Change movement.
When two dimension automatically moves objective table movement, source tablet or target plate are easy to deviate original institute by the effect of inertia
Position so that the second motor by motion controller control it is mobile after, the position where source tablet or target plate and reality
Border needs the position being moved to not to be inconsistent.If source tablet deviates the position at original place, it will lead to the side for choosing the supporting part of worm needle
Edge can not with the coincident of nematode, so as to cause choose worm needle according to the movement of preset path also can not picking to nematode；If mesh
Mark the position that tablet deviates original place, it will cause two dimension to automatically move objective table and moved according to pre-set path
Afterwards, target plate is not at the lower section for choosing worm needle, and the worm needle of choosing of picking to nematode is being moved to the original place of target plate
When position, nematode is not removed, but is adhered on supporting part always, eventually leads to nematode and is not diverted into target plate
On.
In a further embodiment, source tablet and target plate deviate original position in order to prevent, in two-dimentional automatic shifting
There are two fixing grooves 70, the size of fixing groove to be adapted with the size of source tablet and target plate for setting in dynamic object stage.Source is flat
Plate and target plate are separately positioned in two fixing grooves so that source tablet and target plate will not occur partially in moving process
It moves, the position of source tablet and target plate is fixed.Needle holder and two dimension automatically move objective table before movement, choosing on needle holder
Worm needle is consistent with required relative position with the relative position of source tablet；Needle holder and two dimension automatically move objective table in movement
Afterwards, on needle holder to choose worm needle consistent with required relative position with the relative position of target plate, can normally be chosen
Take and place the operation of nematode.
In another embodiment, the quantity of fixing groove can be more than two so that two dimension automatically moves on objective table can be with
Multiple source tablets and target plate are steadily placed, source tablet can be replaced again after repeatedly choosing worm operation in this way and target is flat
Plate, the efficiency for being conducive to choose worm operation improve
In a further embodiment, can be provided with LED light 71 in each fixing groove, LED light can be it is self-powered,
It can also be external power supply.Source tablet and target plate are transparent vessel, are individually positioned in the fixing groove equipped with LED light,
When carrying out calibrating operation and choosing worm operation, need to observe nematode, if the light on periphery is weak, LED using microexamination camera lens
Lamp can provide light source.Such setting is so that calibrating operation and also unaffected in the weak environment of light, energy of choosing worm operation
Enough normal operations.
The nematode of mutation can send out fluorescence in the case where being irradiated with a laser, and be based on such basis, in a preferred embodiment,
Laser light source 72 can be arranged by being automatically moved in two dimension on objective table, specifically, laser light source can be arranged in fixing groove, be
Do not influence the work of LED light, it is preferable that the periphery in source tablet or target plate can be arranged in laser light source, in this way can be with
So that the light source of laser passes through the side wall of source tablet or target plate, enter in source tablet or target plate.Such setting
So that the nematode of mutation sends out fluorescence under the irradiation of laser, user more quickly finds line using the observation of microexamination camera lens
Worm, and then calibrating operation is carried out, effectively improve efficiency.
It, in a further embodiment, can be on microexamination camera lens since laser is strong to the eye irritation of user
Optical filter, such eyes being provided with using protection user are set.
In the particular embodiment, it is additionally provided with control screen 9, the control screen is for microexamination camera lens, LED
The sequence whether lamp, motion controller, laser light source and electric heater start and start.Specifically, control screen and movement
Controller, laser light source, LED light, electric heater and the electrical connection of microexamination camera lens, control and automatical worm button are arranged on screen
And prepare to choose worm button, before pressing preparation and choosing worm button and automatical worm button, user needs to carry out manual calibration, is pressing
After worm button is chosen in preparation, automatic nematode device for sorting carries out automatic calibrating operation, after pressing automatical worm button, automatic nematode
Device for sorting starts to carry out choosing worm operation according to default step.
Referring to Fig. 2, in another embodiment, the vertical bracing strut includes Contraband shape holder and bottom frame, the Contraband shape branch
For frame perpendicular to plane where bottom frame, Contraband shape holder includes the first montant, the second montant and cross bar, and first montant and second are erected
Bar is individually fixed in the corresponding both sides of bottom frame.Microexamination camera lens is fixed on the cross bar of Contraband shape holder, microexamination camera lens
Camera lens ontology plane where top frame is oppositely arranged, and setting two dimension automatically moves objective table in bottom frame.Specifically, two dimension is automatic moves
Dynamic object stage includes support board and two the second motors, and two the second motors can drive support board transverse movement and longitudinal direction respectively
It moves, source tablet and target plate is placed on support board.Such setting allows support board is horizontal and vertical to move.
User carry out manual calibration operation content be：The range calibration to choosing worm needle and source tablet is needed, and to choosing
The range calibration of worm needle and target plate.In order to ensure the accuracy of calibration, need to calibrate above-mentioned two in different times
Calibrate content.Range calibration to choosing worm needle and source tablet needs before choosing worm, to choosing the range calibration of worm needle and target plate
It needs after provoking nematode.
To choosing the range calibration of worm needle and source tablet：The position of the supporting part of worm needle syringe needle is chosen in adjustment on the tablet of source, is made
The side of polypide can just be touched by obtaining the edge of supporting part, while again without impinging on culture medium, the imaging of microexamination camera lens
There is cross foresight, cross foresight to be overlapped with touch points, control screen records the supporting part edge of syringe needle in the visual field of system
Residing height Z1.
To choosing the range calibration of worm needle and target plate：The supporting part of worm needle syringe needle is chosen in adjustment on target plate central point
Position so that target plate is just encountered at the edge of supporting part, and control screen is recorded residing for the supporting part edge of syringe needle
Height Z2.
The content that automatic nematode device for sorting is calibrated automatically is：
It presses preparation and chooses worm button, motion controller can control two dimension and automatically move objective table and needle holder movement so that
Positioned at the position to Z1+2mm above the plate center of source, (needle holder can be raised and avoid tablet needle holder automatically in moving process
Side wall).
It manually controls two dimension to automatically move objective table movement and find polypide to be chosen, makes polypide side wall and the quasi- heart weight of cross
It closes.
Automatically the step of nematode device for sorting progress automatical worm is：
One, two dimension automatically moves objective table movement so that in the cross foresight and source tablet in the micro imaging system visual field
Polypide overlap.
Two, two dimension automatically moves objective table and is moved to the left 1mm.
Three, needle holder moves down to Z1+1.5mm.
Four, two dimension automatically moves objective table and moves right 1.2mm, while needle holder moves down to Z1+0.5mm.
Five, needle holder is up moved to Z2-15mm immediately, and polypide should be stained with by choosing worm needle front end at this time.
Six, two dimension automatically moves objective table and is moved in the range of target plate.
Seven, needle holder moves down to Z2+15mm.
Eight, needle holder, which stops, makes polypide wriggle onto target plate for 10~15 seconds.
Nine, polypide is moved on target plate, and needle holder is up moved to Z2+15mm.
Ten, worm needle is chosen in heating, and 5 seconds cooling.
11, two dimension automatically moves the range that objective table returns to source tablet.
12, needle holder is moved down into the height of Z1+2mm, completes an automatical worm action.
Source tablet and target plate are replaced as needed, are returned to third step, are started the next polypide to be chosen of manual search.
It should be noted that the above description is only the embodiments of the present invention, those skilled in the art once know
Know the basic creative concept of the utility model, then additional changes and modifications can be made to these embodiments, so while
The various embodiments described above have been described herein, but have been not intended to limit the scope of patent protection of the utility model, it is all
It is equivalent structure or equivalent flow shift made based on the specification and figures of the utility model, directly or indirectly uses
In other related technical areas, similarly it is included within the scope of patent protection of the utility model.
Claims (10)
1. a kind of automatic nematode device for sorting, which is characterized in that including vertical bracing strut, microexamination camera lens, needle holder, choose worm
Needle and motion controller；The microexamination camera lens is connect with the top of vertical bracing strut；The syringe needle for choosing worm needle includes holding
Support portion chooses worm needle and is arranged on needle holder；Needle holder is arranged close to the central axes of microexamination camera lens so that the edge of supporting part
It is overlapped with central axes；First motor is provided on needle holder, first motor is for driving moving up and down for needle holder, first motor
It is electrically connected with motion controller, the motion controller is used to control direction and the distance of needle holder movement.
2. automatic nematode device for sorting according to claim 1, which is characterized in that further include that two dimension automatically moves loading
Platform, the two dimension automatically move objective table and are arranged in the bottom end of vertical bracing strut.
3. automatic nematode device for sorting according to claim 2, which is characterized in that the two dimension automatically moves on objective table
Be provided with the second motor, the second motor be used to drive two dimension automatically move objective table movement planar, the second motor
It is electrically connected with the controller, controller is used to control direction and the distance that two dimension automatically moves objective table movement.
4. automatic nematode device for sorting according to claim 2, which is characterized in that the two dimension automatically moves on objective table
It is provided at least two fixing grooves.
5. automatic nematode device for sorting according to claim 2, which is characterized in that the two dimension automatically moves on objective table
There are two LED light, two LED light to be respectively embedded into two fixing grooves for setting.
6. automatic nematode device for sorting according to claim 2, which is characterized in that the two dimension automatically moves on objective table
It is provided at least one laser light source, optical filter is provided on the microexamination camera lens.
7. automatic nematode device for sorting according to claim 1, which is characterized in that be provided with electrical heating in the needle holder
Device, the electric heater choose the heating of worm needle for giving.
8. automatic nematode device for sorting according to claim 1, which is characterized in that the supporting part is grafter shape support
Portion.
9. automatic nematode device for sorting according to claim 8, which is characterized in that the central axes of grafter shape supporting part
The angle of plane is 30 degree to 60 degree where automatically moving objective table with two dimension.
10. automatic nematode device for sorting according to claim 1, which is characterized in that further include control screen, the control
Screen is electrically connected with motion controller and microexamination camera lens, and automatical worm button is provided on control screen and preparation is chosen worm and pressed
Button.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721831498.1U CN207810687U (en) | 2017-12-25 | 2017-12-25 | A kind of automatic nematode device for sorting |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201721831498.1U CN207810687U (en) | 2017-12-25 | 2017-12-25 | A kind of automatic nematode device for sorting |
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| Publication Number | Publication Date |
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| CN207810687U true CN207810687U (en) | 2018-09-04 |
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ID=63330696
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| Application Number | Title | Priority Date | Filing Date |
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| CN201721831498.1U Active CN207810687U (en) | 2017-12-25 | 2017-12-25 | A kind of automatic nematode device for sorting |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107934531A (en) * | 2017-12-25 | 2018-04-20 | 福建上源生物科学技术有限公司 | A kind of automatic nematode device for sorting |
-
2017
- 2017-12-25 CN CN201721831498.1U patent/CN207810687U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107934531A (en) * | 2017-12-25 | 2018-04-20 | 福建上源生物科学技术有限公司 | A kind of automatic nematode device for sorting |
| CN107934531B (en) * | 2017-12-25 | 2024-04-16 | 福建上源生物科学技术有限公司 | Automatic nematode selecting device |
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| Date | Code | Title | Description |
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| GR01 | Patent grant | ||
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| TR01 | Transfer of patent right |
Effective date of registration: 20191224 Address after: Room G311 and G313, Room 388 Ruoshui Road, Suzhou Industrial Park, Jiangsu Province, 215000 Patentee after: Suzhou Shangyuan Biotechnology Co., Ltd. Address before: At the Second Road No. 1 Fuzhou international financial center 15 layer 1503 units in the Taijiang District of Fuzhou City, Fujian province 350000 Patentee before: Fujian Shang Yuan biological science and Technology Co., Ltd. |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20201130 Address after: Unit 1503, 15 / F, IFC Fuzhou International Financial Center, No.1 Wanglong Er Road, Taijiang District, Fuzhou City, Fujian Province Patentee after: FUJIAN SUNYBIOTECH Corp. Address before: Room G311 and G313, Room 388 Ruoshui Road, Suzhou Industrial Park, Jiangsu Province, 215000 Patentee before: SUZHOU SUNYBIOTECH Co.,Ltd. |
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| TR01 | Transfer of patent right |