CN205880376U - Animal mesenterium microcirculation observation system - Google Patents

Abstract

The utility model discloses an observation system for animal mesentery microcirculation, which comprises an experimental device and an observation device; the observation device includes a microscope, and an observation position adjustment device is arranged on the base of the microscope, and the observation position adjustment device is two sides of the vertical column of the microscope along the horizontal plane. The structure is fine-tuned in two directions perpendicular to each other; the microscope is a movable microscope, which realizes the separation of the microscope and the experimental device. Using the above technical scheme, the animal mesenteric microcirculation observation system has ingenious and reasonable design, convenient operation, stable technology, and good microcirculation observation effect, which better solves the technical problem of simulating the intraperitoneal environment of mesenteric microcirculation in vitro; The way to fix the observation object will not cause the observation object to be involved, making it more convenient to adjust the observation field of view and position, and the observation effect is better.

Description

Animal mesenteric microcirculation observation system

technical field

The utility model belongs to the technical field of medical scientific research and teaching experiment equipment, and relates to an observation instrument for animal tissues. More specifically, the utility model relates to an animal mesenteric microcirculation observation system, mainly an animal mesenteric microcirculation observation system in vivo.

Background technique

The animal mesenteric microcirculation observation system is an important device for microcirculation research and observation, and is widely used in medical research and teaching. The structural design and technical quality of the observation system directly affect the effect and results of microcirculation observation.

In the prior art, the animal mesenteric microcirculation observation system is imperfectly designed and often breaks down, which can no longer adapt to today's experimental teaching and scientific research.

For example, when adjusting and observing different fields of view of the microcirculation, the current methods all use the movement of the objects in the microcirculation pool, while the observation device is fixed and does not move. This can easily cause the involvement of the entire mesentery and affect the fixation of the microcirculation field of view, thereby affecting the results of microcirculation observation.

As another example, most of the microcirculation perfusion devices use the constant temperature thermocouple method at present, and the device has the disadvantages of being prone to failure and damage and uneven heating; the moisturizing and perfusion methods are mostly manually dripped, and these methods are often cumbersome to operate. Unstable technology, complex devices, affecting the observation environment, inaccurate positioning of the perfusate, and inability to directly perfuse the mesentery.

Utility model content

The problem solved by the utility model is to provide an animal mesenteric microcirculation observation system, the purpose of which is to realize immobility of mirror animals and improve the observation effect of mesenteric microcirculation.

In order to achieve the above object, the technical scheme that the utility model takes is:

The animal mesenteric microcirculation observation system of the present utility model includes an experimental device and an observation device; the observation device includes a microscope, and the base of the microscope is provided with an observation position adjustment device, and the observation position adjustment device is for the The column of the microscope is fine-tuned along two mutually perpendicular directions on the horizontal plane; the microscope is a movable microscope, which realizes the separation of the microscope and the experimental device.

The microscope is provided with a structure for rotating the column of the microscope and a structure for locking it.

The microscope is provided with a microscopic camera.

The observation device is provided with a computer installed with microcirculation observation and analysis software, and the image signal generated by the observation device is sent to the computer through a circuit.

The experimental device is provided with an operating table, a microcirculation pool and a constant temperature water bath; a sponge is arranged in the microcirculation pool, and the test object is placed on the sponge, and the microcirculation pool passes through two A rubber tube communicates with the constant temperature water bath.

The observation position adjustment device is a screw-type adjustment structure, and the screw-type adjustment structure is provided with a knob.

The constant temperature water bath is a digital display constant temperature water bath.

The microcirculation pool is made of plexiglass and bonded with chloroform or other adhesives.

The experimental device is provided with a thermometer with a length of 12 cm and a measuring range of 50°C.

The lighting fixture in the observation device adopts the built-in 3wLED transmission cold light lamp of the microscope.

The utility model adopts the above-mentioned technical scheme, the animal mesenteric microcirculation live observation system has ingenious and reasonable design, convenient use and operation, stable technical state, good microcirculation observation effect, and well solves the technical problem of simulating the intraperitoneal environment of the mesenteric microcirculation in vitro. It is an ideal observation system for animal mesenteric microcirculation. The observation device is moved while the observation object is fixed (that is, the mirror animal does not move), so that the observation object (the microcirculation of the mesentery) will not be dragged and moved, so that the observation environment of the entire mesentery microcirculation is fixed, and it is easier to adjust and observe different fields of view of the entire microcirculation. It is convenient and has better observation effect; when operating the object to be inspected, the observation device can be removed for easy operation; the constant temperature circulating water heating method is adopted, which is simple, stable, adjustable in temperature and uniform in heating. Advantages, it better solves the thermal insulation problem of the microcirculation observation system. The perfusion ring (the radius of the perfusion ring is equivalent to that of the mesenteric ring) is used to infuse drip irrigation and drainage of the perfusate plus the method of covering with plastic wrap. This method is simple and stable, does not affect the observation environment, and has the functions of heat preservation, moisturizing, perfusion and fixation.

Description of drawings

The contents of the drawings and the marks in the drawings are briefly explained as follows:

Fig. 1 is the structural representation of the microcirculation experiment device of the present utility model;

Fig. 2 is the structural representation of observation device of the present utility model;

Fig. 3 is the overall structural representation of the utility model;

Fig. 4 is a schematic diagram of the operating table in the utility model.

Labeled in the figure:

1. Microcirculation pool, 2. Constant temperature water bath, 3. Sponge, 4. Plastic wrap, 5. Rubber tube, 6. Object to be inspected, 7. Observation device, 8. Thermometer, 9. Experimental device, 10. Observation position Adjusting device, 11, computer, 12, microscope, 13, microscopic camera, 14, column, 15, knob, 16, base, 17, perfusion ring.

detailed description

Next, with reference to the accompanying drawings, through the description of the embodiments, the specific implementation of the utility model will be further described in detail, so as to help those skilled in the art to have a more complete, accurate and in-depth understanding of the inventive concept and technical solutions of the utility model .

The structure of the utility model as shown in Fig. 1, Fig. 2, Fig. 3 and Fig. 4 is a kind of animal mesenteric microcirculation observation system. The utility model is an animal mesenteric microcirculation live observation system for medical scientific research and teaching, which is used for observing the microcirculation of an object 6, wherein the object 6 is the mesentery of an animal; the animal mesenteric microcirculation observation system of the utility model The system includes an experimental device 9 and an observation device 7 ; the observation device 7 includes a microscope 12 .

In order to solve the problems existing in the current known technology described in the background technology part of this description and overcome its defects, and realize the invention purpose of improving the observation effect of mesenteric microcirculation, the technical scheme adopted by the utility model is:

As shown in Figure 1, in this utility model provided in this utility model animal mesenteric microcirculation observation system, the utility model animal mesenteric microcirculation observation system, the base 16 of described microscope 12 is provided with observation position adjusting device 10 , the observation position adjustment device 10 is a structure that fine-tunes the column 14 of the microscope along two mutually perpendicular directions on the horizontal plane; the microscope 12 is a movable microscope, and realizes that the microscope 12 and the The experimental device 9 is separated.

The whole microscope can be moved in two directions (XY) to observe different fields of view. At the same time, the animal experiment platform and the observation device can be separated, and the observation can be carried out after the animal experiment is completed.

The microscope 12 is provided with a structure for rotating the column 14 of the microscope and a structure for locking it. It is convenient for animal experiment operation.

The microscope 12 is provided with a microscopic camera 13 .

The observation device 7 is provided with a computer 11 installed with microcirculation observation and analysis software, and the image signal generated by the observation device 7 is sent to the computer 11 through a circuit. Record and analyze the observed images.

The experimental device 9 is provided with an operating table, a microcirculation pool 1 and a constant temperature water bath 2; the microcirculation pool 1 is provided with a sponge 3, and the test object 6 is placed on the sponge 3 , the microcirculation pool 1 communicates with the constant temperature water bath 2 through two rubber tubes 5 .

The observation device is moved while the observation object is fixed, so that the observation object will not be involved and the observation effect will not be affected, making it more convenient to adjust the observation field of view and the observation effect is better.

For the regulating device, a more specific technical solution is as follows:

The adjustment structure is a screw-type adjustment structure, and the adjustment structure is provided with a knob 15 .

The observation system of the present utility model provides a camera imaging device, and the computer 11 of the system also includes a display screen, which is convenient for the operation of the experimenter, and observes the dynamic change of the mesenteric microcirculation while monitoring the physiological signal change;

The image obtained by the observation device 7 is analyzed by the microcirculation observation and analysis software;

Two mutually perpendicular adjustment structures are adopted to realize the adjustment of the observation device 7 at any position on the horizontal plane, so as to observe the mesentery more comprehensively. The observation device is moved while the observation object is fixed (that is, the mirror animal does not move), so that the observation object will not be involved, making it more convenient to adjust the observation field of view and position, and the observation effect is better.

The imaging and imaging device, that is, the observation device 7, can also rotate around its own column, which can facilitate the operation of the mesentery.

The utility model adopts high-resolution color digital camera technology, USB2.0 interface to collect image data, the static image can reach up to 13 million pixels (4280×3024), and the real-time dynamic video can reach DVD quality (640×480), and the image is clearer Smooth, easy and convenient installation.

The main functions of the utility model include: integrated observation of microcirculation images and physiological parameters, microcirculation observation of human body armor (Shanghai 94 method and Tian's method), etc.

Main Specifications:

1. Microscopic imaging device, objective lens magnification: 10×10, 10×40;

2. 3W LED transmits cold light, and the brightness can be adjusted;

3. Adjustable X-Y two-dimensional mobile microscope base;

4. Professional USB2.0 color digital camera;

5. Sampling resolution is 768×576, supporting simultaneous recording and playback of waveform and video;

6. Supporting constant temperature controller, 400W heating power, temperature control accuracy 0.1 degree.

7. Provide LED episcopic light source and human finger rest, which can cooperate with human armor microcirculation analysis software to analyze the results.

The adjustment structure includes a base and a sliding platform slidingly matched with it, wherein the sliding platform of one adjustment structure is the base of the other adjustment structure.

Observation device 7 is installed on the moving slide table of the adjustment structure above, realizes the arbitrary adjustment of horizontal position.

The adjustment structure is a screw nut structure, wherein the end of the screw rod is provided with an adjustment knob.

The screw nut structure is adopted, and the position of the observation device 7 is adjusted by rotating the adjusting knob. The operation is convenient and stable. After adjusting to any position, it will not be displaced by other factors, so that the observation work can be carried out smoothly.

As shown in Figure 2, the utility model provides supporting device also includes:

The animal mesenteric microcirculation observation system of the present utility model is provided with microcirculation tank 1 and constant temperature water bath tank 2, is provided with sponge 3 in described microcirculation tank 1, and described subject 6 is placed in described sponge 3, the microcirculation pool 1 communicates with the constant temperature water bath 2 through two rubber tubes 5 .

The microcirculation pool 1 is connected with the operating table where the animals are placed, so that the mesentery of the animal can be obtained from the operating table, and then placed in the microcirculation pool 1 . The perfusion ring 17 (the radius of the perfusion ring is equivalent to that of the mesentery ring) is used to infuse drip irrigation and drainage of the perfusate, and the method of covering with fresh-keeping film is adopted.

Adopting the above-mentioned technical scheme, the animal mesenteric microcirculation observation system has ingenious and reasonable design, convenient operation, stable technology, and good microcirculation observation effect, which better solves the technical problem of simulating the intraperitoneal environment of mesenteric microcirculation in vitro, and provides a better way for mesenteric microcirculation Ideal viewing system. The constant temperature circulating water heating method is adopted. This method has the advantages of simplicity, stability, adjustable temperature, and uniform heating, which better solves the problem of heat preservation of the microcirculation observation system.

In addition, it is also equipped with accessories such as infusion devices.

The size of the above observation system can be scaled up or down according to the size of the animal.

According to the experimental conditions and requirements, the observation and analysis equipment and software required by the observation device 7, microphotography camera, microscopic image analysis system, etc. are equipped.

The microcirculation pool 1 adopted in the utility model is made of plexiglass, and is bonded and formed with chloroform or other adhesives. The microcirculation pool 1 is made of a plexiglass plate with a thickness of 1, 2, 5, and 7 mm.

The observation system is provided with a thermometer 8 with a length of 12 cm and a measuring range of 50°C. The thermometer 8 is set on the microcirculation pool 1 close to the object 6, so as to monitor the temperature of the microcirculation pool in real time.

The lighting fixture in the observation system of the utility model adopts the 3w LED transmission cold light lamp built in the microscope, and the brightness can be adjusted.

The constant temperature water bath 2 adopted by the utility model is a digital display constant temperature water bath. The digital display constant temperature water bath is widely used for drying, concentrating, distilling, impregnating chemical reagents, impregnating medicines and biological products, and can also be used for water bath constant temperature heating and other temperature tests. Sanitation, biochemical laboratories, analysis rooms, necessary tools for education and scientific research. Its structural characteristics are:

1. The studio water tank is made of stainless steel, which has excellent corrosion resistance;

2. High precision temperature control, digital display, automatic temperature control;

3. Easy to operate and safe to use.

Specifically, the constant temperature water bath 2 adopts the ZC-10 super digital display constant temperature water bath produced by Ningbo Tianheng Instrument Factory.

The technical parameters of the ZC-10 super digital constant temperature water bath are:

Temperature range: room temperature to 100°C; digital display resolution: 0.1°C; temperature fluctuation: ±0.1°C; tank volume: 10L; working tank size: 280×180×200mm; tank opening: 160×160mm; tank depth: 200mm ;Pump flow: 6L/min; Dimensions: 260×320×395mm;

The ZC-10 super digital constant temperature water bath has the following technical characteristics:

1. With advanced internal circulation/external circulation pump system, the internal circulation ensures uniform and constant temperature, and the external circulation pump can lead the heating liquid in the tank to the external experiment window of the heating or constant temperature machine;

2. LED dual windows respectively display the temperature measurement value and temperature setting value, the digital display resolution is high, and the touch button is easy to operate;

3. It has the function of correcting the deviation of the measured value, and the temperature accuracy is high;

4. With over-temperature protection function and digital timing heating system, the timing heating time can be set within 0-999 minutes;

5. Desktop structure, small size;

6. The constant temperature water tank has the function of low liquid level monitoring and protection: when the liquid medium in the working tank is gradually reduced due to volatilization or the aging and rupture of the external circulation pump pipeline or other reasons, so that the liquid level drops, the low liquid level protection device The electric heating system is automatically cut off, and an alarm sound prompts at the same time to ensure the safety of the instrument and samples.

In summary, the animal mesenteric microcirculation observation system of the present utility model mainly includes: 1. heat preservation, moisturizing and perfusion devices for microcirculation; 2. optical camera, built-in LED cold light source; 3. universal adjustment of the observation device; 4. , Using a computer and its imaging device, microcirculation observation and analysis software.

Referring to Fig. 1, Fig. 2 and Fig. 3, adopt the corresponding experimental method of above-mentioned system of the present utility model to be:

Turn on the constant temperature water bath 2 to make the temperature of the microcirculation pool 1 reach the desired temperature, put the sponge 3 into the pool, and then pour the Tyrode's solution warmed by the water bath into the pool, so as to completely submerge the sponge 3, and keep warm for 10 minutes.

Place the test object 6 (the tested animal) on the operating table, make a 6 cm long longitudinal mid-abdominal incision next to the rectus abdominis on one side, open the abdominal cavity and push the upper abdominal organs to the opposite side, and select a section For small intestine loops with large freeness and abundant microcirculation, spread them on the sponge 3 in the microcirculation pool 1, and then cover the perfusate ring on the mesentery; the radius of the perfusate ring is equivalent to that of the mesentery ring; finally Paste plastic wrap 4 on the entire perfusate ring and mesentery.

The preservative film 4 covers and sticks and plays heat preservation, moisture retention, fixing effect to mesentery. And the plastic wrap 4 is a good transparent material, which can be observed with an observation device conveniently.

Then, a drainage line and a perfusion liquid ring are placed in the microcirculation pool 1 with a cotton thread to form a group of perfusion and drainage microcirculation devices. The number of strands of the drainage line determines the amount of drainage, the more the number of strands, the greater the amount of drainage.

During the observation process, by operating the adjustment knob, the positions in two directions on the horizontal plane of the observation device 7 are adjusted to observe microscopic images at different positions. At the same time, the height of the objective lens can also be manipulated to adjust the field of view of observation; in addition, the objective lens can be rotated 180° clockwise or counterclockwise along the column, making the operation of animal mesentery more convenient.

The utility model has been exemplarily described above in conjunction with the accompanying drawings. Obviously, the specific implementation of the utility model is not limited by the above-mentioned methods, as long as various insubstantial improvements are made by adopting the method concept and technical solutions of the utility model, or Directly applying the ideas and technical solutions of the utility model to other occasions without improvement is within the protection scope of the utility model.

Claims (9)

1. animal mesenteric microcirculation observation system, comprises experimental device (9), observation device (7); Described observation device (7) comprises special-purpose microscope (12), is characterized in that: described special-purpose microscope (12) The base (16) is provided with an observation position adjustment device (10), and the observation position adjustment device (10) is a structure for fine-tuning the column (14) of the microscope in two mutually perpendicular directions along the horizontal plane; The special-purpose microscope (12) is a movable microscope, which separates the special-purpose microscope (12) from the experimental device (9); the observation position adjustment device (10) is a spiral adjustment structure, The screw-type adjustment structure adopts a screw nut structure, wherein the end of the screw rod is provided with a knob (15). 2. according to the described animal mesenteric microcirculation observation system of claim 1, it is characterized in that: described special-purpose microscope (12) is provided with the structure that makes the column (14) of described special-purpose microscope rotate and its locking structure. 3. The animal mesenteric microcirculation observation system according to claim 1, characterized in that: the special microscope (12) is provided with a microscopic camera (13). 4. according to the described animal mesenteric microcirculation observation system of claim 1, it is characterized in that: described observation device (7) is provided with the computer (11) that microcirculation observation analysis software has been installed, described observation device (7) ) The image signal generated by the circuit is sent to the computer (11). 5. according to the described animal mesenteric microcirculation observation system of claim 1, it is characterized in that: described experimental device (9) is provided with operating table, microcirculation pool (1) and constant temperature water bath (2); The microcirculation pool (1) is provided with a sponge (3), and the object to be tested (6) is placed on the sponge (3), and the microcirculation pool (1) is connected to the microcirculation pool (1) through two rubber tubes (5). The constant temperature water bath (2) is connected. 6. The animal mesenteric microcirculation observation system according to claim 5, characterized in that: the constant temperature water bath (2) is a digital display constant temperature water bath. 7. The animal mesenteric microcirculation observation system according to claim 5, characterized in that: the microcirculation pool (1) is made of plexiglass and bonded with chloroform or other adhesives. 8. The animal mesenteric microcirculation observation system according to claim 1, characterized in that: said experimental device (9) is provided with a thermometer (8) with a length of 12 cm and a measuring range of 50°C. 9. The animal mesenteric microcirculation observation system according to claim 1, characterized in that: the lighting fixture in the observation device (7) adopts a 3wLED transmission cold light lamp built into the microscope.