CN207040657U - A kind of in vitro low temperature oxygen carrying machine perfusion device of toy liver - Google Patents

Abstract

The utility model provides an isolated low-temperature oxygen-carrying mechanical perfusion device for a small animal liver, which comprises an organ preservation device, a centrifugal pump, an oxygenator, a debubbling device, a flow controller, and a pressure sensor that are sequentially connected through a heparin-coated pipeline; A three-way pipe is respectively arranged between the oxygenator and the centrifugal pump, and between the oxygenator and the debubbler. The system is powered by a centrifugal pump to continuously perfuse the liver of small animals with low-temperature oxygenation cycle. This equipment is easy to operate, has a short perfusion path, and the cycle design greatly saves the amount of perfusate. It is especially suitable for low-temperature perfusion of small animal livers.

Description

A low-temperature oxygen-carrying mechanical perfusion device for isolated small animal liver

technical field

The utility model relates to the field of evaluation and repair of transplanted surgical donor livers, in particular to an isolated low-temperature oxygen-carrying mechanical perfusion device for small animal livers.

Background technique

Since the 21st century, organ transplantation has become the most effective means of treating terminal diseases. Organ transplantation has always been hailed as a major technology that changed human life and biological science in the 20th century. At present, China's organ transplantation business is developing rapidly, which not only promotes the development of high-tech and related sciences, but also promotes the progress of medical ethics and the transformation of social values. Organ transplantation has always been at the forefront of life sciences, and its complexity and difficulty represent a high-tech technology in world medicine. However, the lack of organ resources seriously restricts the further development of transplantation.

In order to alleviate the shortage of donor organ resources, the China Health Planning Commission and the Red Cross Society of China launched a pilot project of human organ donation in 2010, and in 2013, organ donation after the death of citizens was included in the important agenda of my country's transplant work. At the same time, due to the shortage of liver donors, the status of marginal liver donors as a way to expand the source of liver donors has attracted more and more attention. With the improvement of organ perfusion fluid, preservation methods and surgical techniques, the application of marginal donor livers has gradually expanded.

However, clinical retrospective studies have shown that donors after cardiac death (donation after cardiac death, DCD) have longer warm ischemic injury, delayed recovery of renal function (delayed graft function, DGF) and primary non-function after transplantation. (primary nonfunction, PNF) and microcirculatory disorders are more serious than those of brain-dead donors (donation after brain death, DBD). Post-transplantation complications (PNF, IPF, rejection, vascular and biliary complications, HBV recurrence, etc.) brought about by marginal donor livers (fatty livers, aged livers, hepatitis donors, etc.) are still a severe problem we face challenge. At the same time, donor organs suffer from primary diseases, warm ischemia, cold ischemia, cold preservation injury, reperfusion injury and other factors during the period from acquisition to transplantation, and the quality of donor organs varies. Therefore, how to assess, maintain the quality of donor organs, and repair damage is another important challenge for the development of transplantation, and it is also one of the research directions in the field of organ transplantation today.

The quality evaluation and functional restoration of marginal liver donors, especially DCD warm ischemic liver donors, are current research hotspots. I have designed a low-temperature oxygen-carrying mechanical perfusion device for the liver of small animals, and applied the low-temperature oxygen-carrying mechanical perfusion method to intervene, and studied the evaluation and repair of the quality of the donated liver by low-temperature oxygen-carrying mechanical perfusion.

Utility model content

The purpose of the utility model is to repair the marginal donor liver in a low-temperature oxygen-carrying mechanical perfusion system so that it meets the needs of transplantation.

The purpose of this utility model can be achieved in the following ways:

An isolated low-temperature oxygen-carrying mechanical perfusion device for a small animal liver, comprising an organ preservation device, a centrifugal pump, an oxygenator, a debubbler, a flow controller, and a pressure sensor that are sequentially connected through a heparin-coated pipeline; the oxygenator There are three-way pipes between the oxygenator and the centrifugal pump, and between the oxygenator and the debubbler, which are used for sampling and testing the actual partial pressure of oxygen entering the liver.

As a preferred item: the organ preservation device has a detachable structure, including a detachable internal organ storage box and an external low-temperature box; the organ storage box is provided with a portal vein connector and a bile duct connector; There is a temperature control circuit board; at the same time, there is a temperature monitor at the bottom of the organ storage box. The temperature probe is placed in the organ storage box to monitor the temperature of the perfusate in real time, and the temperature can be set by itself to activate the early warning device. The hepatic portal vein connects with heparin through the portal vein. The coated pipe connection forms the filling inlet.

As a preferred option: the portal vein connection is a two-way connection, and the pressure sensor is connected to the portal vein connection.

As a preferred item: a bile collection device is also included, and the bile collection device communicates with the bile duct connection piece; the bile duct connection piece is a two-way connection piece.

As a preferred option: the interior of the debubbler is a mesh-like fibrous filter membrane, and its top is equipped with vent holes.

Compared with the prior art, the utility model has the following advantages: 1. The unique advantage of low-temperature oxygen-carrying mechanical perfusion compared with cold storage is that it can not only provide nutrients for nutritional support, promote the discharge of toxins in the body, but also trigger a series of protection mechanisms to cause 2. Provide oxygen to the ischemic liver by means of preservation fluid carrying oxygen to repair the consumption of ATP; at the same time, Drugs such as anti-oxidation and improving microcirculation can be added to the perfusion fluid to perform drug intervention treatment on ischemic liver; 4. The device of the air-removing device is improved in this system, and the inside is a mesh-like fiber filter membrane, which can Filter tiny blood clots and other impurities, and continuously purify the perfusate; 5. The equipment of this system is easy to operate, the perfusion path is short, and the cycle design greatly saves the amount of perfusate, which is suitable for low-temperature perfusion of small animal livers.

Description of drawings

Fig. 1 is the structural representation of the utility model;

Among them: 1 is an organ preservation device, 2 is a heparin-coated pipeline, 3 is a centrifugal pump, 4 and 6 are three-way tubes, 5 is an oxygenator, 7 is a debubbling device, 8 is a flow controller, and 9 is a pressure Sensor, 10 is an organ storage box, 11 is a cryogenic box, 12 is a bile collection device, a portal vein connector 13, and a bile duct connector 14.

detailed description

Below in conjunction with embodiment and accompanying drawing, the utility model is further described.

An isolated low-temperature oxygen-carrying mechanical perfusion device for small animal livers, including an organ preservation device 1, a centrifugal pump 3, an oxygenator 5, a debubbler 7, a flow controller 8, and a pressure sensor, which are sequentially connected through a heparin-coated pipeline 2 9. Between the oxygenator 5 and the centrifugal pump 3, and between the oxygenator 5 and the debubbler 7, there are respectively three-way pipes 4 and 6, which are used for sampling and testing the actual partial pressure of oxygen entering the liver.

The organ preservation device 1 is a detachable structure, including a detachable internal organ storage box 10 and an external low-temperature box 11; the organ storage box 10 is provided with a portal vein connecting piece 13 and a bile duct connecting piece 14; the low-temperature box There is a temperature control circuit board at the bottom of 11; at the same time, a temperature monitor is installed at the bottom of organ storage box 10, and the temperature probe is placed in organ storage box 10 to monitor the temperature of the perfusate in real time, and the temperature can be set by itself to activate the early warning device.

The portal vein connector 13 is a two-way connector, the pressure sensor 9 is connected to the portal vein connector, and the hepatic portal vein is connected to the heparin-coated pipeline through the portal vein connector 13 to form an infusion inlet.

Also includes a bile collection device 12, the bile collection device 12 communicates with the bile duct connector 14;

The inside of the air-removing device 7 is a screen-like fiber filter membrane, and its top facility has vent holes. After the perfusate enters the air-removing device, impurities such as tiny blood clots are filtered out through the internal drying screen. The gas is exhausted through the exhaust hole.

First, take an appropriate amount of perfusion solution and add it to the organ storage box in the oxygen-carrying mechanical perfusion system. At this time, connect the perfusion pipeline 2 between the organ preservation device 1 and the centrifugal pump 3, and the end of the organ preservation device 1 is not directly connected to the organ preservation device. 1 connection, but located below the liquid level of the perfusate in the organ storage box. Driven by the centrifugal pump, the perfusate passes through the oxygenator and debubbler in sequence, and after removing air bubbles, it flows back to the organ storage box along the perfusion pipeline, so that the entire perfusion The system pipeline is filled with perfusate, and the centrifugal pump stops working. After the donor liver is harvested, it is placed in the liver perfusate in the organ storage box at 4°C. The water mixture keeps the donor liver at a low temperature.

First start the centrifugal pump, the perfusate is driven by the centrifugal pump, and enters the pipeline system through the oxygenator and debubbler in turn. After the whole pipeline system is filled with perfusate and the air is exhausted, the centrifugal pump is turned off. At this time, the hepatic portal vein passes through the portal vein. The connector is connected to the perfusion pipeline, and the centrifugal pump is started again. The perfusate starts to circulate and perfuse from the portal vein. The flow direction of the perfusate is shown by the thick arrow in the solid line in the figure. The perfusate enters the liver through oxygenation and other processes to participate in its evaluation and repair process. The utility model uses HTK (histidine-tryptophan-ketoglutarate solution) to perfuse the liver, the centrifugal pump provides power, the oxygenator oxygenates the HTK, pumps it into the liver through the pipeline, and the HTK flowing out of the hepatic vein flows back through the extrahepatic heparin-coated pipeline to the centrifugal pump to complete the extracorporeal circulation process.

Although this manual uses a lot of 1 for organ preservation devices, 2 for heparin-coated pipes, 3 for centrifugal pumps, 4 and 6 are tee tubes, 5 for oxygenators, 7 for debubblers, and 8 for flow rates Controller, 9 is a pressure sensor, 10 is an organ storage box, 11 is a cryogenic box, 12 is a bile collection device, portal vein connector 13, bile duct connector 14 and other terms, but the possibility of using other terms is not excluded. These terms are only used to describe the essence of the utility model more conveniently, and interpreting them as any kind of additional limitation is contrary to the spirit of the utility model.

It should be understood that the parts not described in detail in this specification belong to the prior art.

It should be understood that the above-mentioned descriptions for the preferred embodiments are relatively detailed, and cannot therefore be considered as limiting the protection scope of the utility model patent. In the case of the scope of protection of the new claims, replacement or deformation can also be made, all of which fall within the protection scope of the utility model, and the scope of protection of the utility model should be based on the appended claims.

Claims (5)

1. A low-temperature oxygen-carrying mechanical perfusion device for an isolated small animal liver, characterized in that it includes an organ preservation device (1), a centrifugal pump (3), and an oxygenator (5) sequentially connected through a heparin-coated pipeline (2). ), the bubble remover (7), the flow controller (8), the pressure sensor (9); between the oxygenator (5) and the centrifugal pump (3), the oxygenator (5) and the bubble remover ( 7) There are three-way pipes (4, 6) respectively arranged between them. 2. The small animal liver isolated hypothermic mechanical perfusion device with oxygen carrying device according to claim 1, characterized in that: the organ preservation device (1) is a detachable structure, including a detachable internal organ storage box (10) and an external thermostatic box (11); the organ storage box (10) is placed with a portal vein connector (13) and a bile duct connector (14); the bottom of the thermostatic box (11) is provided with a temperature control circuit board; the A temperature probe is arranged on the organ storage box (10). 3. The isolated small animal liver low-temperature oxygen-carrying mechanical perfusion device according to claim 2, characterized in that: the portal vein connection (13) is a two-way connection, and the pressure sensor (9) and the portal vein connection (13) CONNECTIONS. 4. The mechanical perfusion device for isolated small animal liver at low temperature with oxygen according to claim 2, characterized in that it further comprises a bile collection device (12), and the bile collection device (12) communicates with the bile duct connector (14) ; The bile duct connector (14) is a two-way connector. 5. The low-temperature oxygen-carrying mechanical perfusion device for isolated small animal livers according to claim 1, characterized in that: the interior of the debubbler (7) is a mesh-like fiber filter membrane, and the top facility has an exhaust hole.