CN206563748U - A kind of antibody incubation device suitable for western blot test - Google Patents
A kind of antibody incubation device suitable for western blot test Download PDFInfo
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- 238000011534 incubation Methods 0.000 title claims abstract description 76
- 238000001262 western blot Methods 0.000 title claims description 15
- 238000007789 sealing Methods 0.000 claims abstract description 11
- 238000012360 testing method Methods 0.000 claims abstract description 11
- 238000003119 immunoblot Methods 0.000 claims abstract description 6
- 238000002474 experimental method Methods 0.000 abstract description 11
- 238000010586 diagram Methods 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 108090000623 proteins and genes Proteins 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 239000007788 liquid Substances 0.000 description 3
- 230000000903 blocking effect Effects 0.000 description 2
- 238000001962 electrophoresis Methods 0.000 description 2
- 238000009396 hybridization Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 239000002033 PVDF binder Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000001502 gel electrophoresis Methods 0.000 description 1
- 238000011173 large scale experimental method Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920002981 polyvinylidene fluoride Polymers 0.000 description 1
- 238000004451 qualitative analysis Methods 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 238000010186 staining Methods 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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Abstract
本实用新型提供一种适用于免疫印迹试验的抗体孵育装置，包括箱体、箱盖和抗体孵育盒；箱体中，箱体相对的两侧设置多组工型孔，箱体内设有调节机构，箱体顶部设置与抗体孵育盒相适应的孔；抗体孵育盒中，盒体顶端设置凹槽，盒盖底端设置与凹槽相适应的凸起一，盒盖与盒体通过卡槽连接；抗体孵育盒通过孔放置在箱体内，箱体上方设有箱盖，箱盖和箱体通过活动合页连接；该孵育装置通过将抗体孵育盒小型化并置于可封闭的箱体中，确保了抗体孵育盒的稳定性，工型孔和调节机构的作用使得抗体孵育盒可以方便地从箱体中取出，采用推拉天窗式的抗体孵育盒有效解决了排气和封闭的问题，在医疗实验领域具有广泛的实用性。
The utility model provides an antibody incubation device suitable for immunoblotting test, which comprises a box body, a box cover and an antibody incubation box; Mechanism, the top of the box is provided with a hole suitable for the antibody incubation box; in the antibody incubation box, the top of the box is provided with a groove, and the bottom of the box cover is provided with a protrusion suitable for the groove, and the box cover and the box body pass through the slot Connection; the antibody incubation box is placed in the box through a hole, the box is provided with a cover above the box, and the box cover and the box are connected by a movable hinge; the incubation device miniaturizes the antibody incubation box and places it in a closeable box , to ensure the stability of the antibody incubation box, the function of the I-shaped hole and the adjustment mechanism makes the antibody incubation box can be easily taken out of the box, and the sliding skylight-type antibody incubation box is used to effectively solve the problem of exhaust and sealing. It has wide applicability in the field of medical experiments.
Description
技术领域technical field
本实用新型属于医疗实验装置的技术领域，更具体的，本实用新型涉及一种抗体孵育装置的技术领域。The utility model belongs to the technical field of medical experiment devices, more specifically, the utility model relates to the technical field of an antibody incubation device.
背景技术Background technique
免疫印迹试验，也称蛋白质印迹法、Western Blot，它是分子生物学中一种常用的实验方法。其基本原理是通过特异性抗体对凝胶电泳处理过的细胞或生物组织样品进行着色，通过分析着色的位置和着色深度获得特定蛋白质在所分析的细胞或组织中的表达情况的信息，即将目的蛋白进行电泳分离后转移至PVDF膜上，再与已知的蛋白抗体杂交，最后通过酶系统的显色反应对目的蛋白质进行定性和定量分析。它主要包括5个步骤：电泳、转移、封闭、杂交和显色，其中封闭和杂交过程也称抗体孵育过程。Western blot test, also known as Western blotting and Western Blot, is a commonly used experimental method in molecular biology. The basic principle is to stain the cells or biological tissue samples treated by gel electrophoresis through specific antibodies, and to obtain information about the expression of specific proteins in the analyzed cells or tissues by analyzing the position and depth of staining, that is, the purpose After the protein is separated by electrophoresis, it is transferred to the PVDF membrane, then hybridized with known protein antibodies, and finally the qualitative and quantitative analysis of the target protein is carried out through the color reaction of the enzyme system. It mainly includes 5 steps: electrophoresis, transfer, blocking, hybridization and color development, and the blocking and hybridization process is also called antibody incubation process.
在进行大批量实验时，普通的孵育盒便可以满足需求，但是实验过程中更多的是一些小实验，尤其是在预实验中，选取某一个蛋白作一项实验时，实验步骤复杂无序，孵育的时候常常因为没有合适的孵育器皿，会出现孵育盒排气不易、密封不严导致抗体挥发、受到振动液体洒出的情况。因此，亟需一种合适的抗体孵育装置。When conducting large-scale experiments, ordinary incubation boxes can meet the needs, but there are more small experiments in the experimental process, especially in the pre-experiment, when a certain protein is selected for an experiment, the experimental steps are complicated and out of order , When incubating, often because there is no suitable incubating vessel, the incubating box is not easy to exhaust, the seal is not tight, causing the antibody to volatilize, and the vibration liquid is spilled. Therefore, there is an urgent need for a suitable antibody incubation device.
发明内容Contents of the invention
针对现有技术现状的不足，为了解决目前没有适合于小实验的抗体孵育器皿，会出现孵育盒排气不易、密封不严导致抗体挥发、受到振动液体洒出的技术问题，本实用新型提供了一种适用于免疫印迹试验的抗体孵育装置，与现有技术相比，该孵育装置结构简单，使用方便，通过将抗体孵育盒小型化并置于可封闭的箱体中，确保了抗体孵育盒的稳定性，工型孔和调节机构的作用使得抗体孵育盒可以方便地从箱体中取出，采用推拉天窗式的抗体孵育盒有效解决了排气和封闭的问题，实验体系小节省抗体等试剂的使用量，便于多次重复实施，在医疗实验领域具有广泛的实用性。Aiming at the shortcomings of the current state of the art, in order to solve the technical problems that there are no antibody incubation vessels suitable for small experiments, the incubation box is not easy to exhaust, the seal is not tight, resulting in antibody volatilization and vibration liquid spilling, the utility model provides An antibody incubation device suitable for western blotting test. Compared with the prior art, the incubation device has a simple structure and is convenient to use. By miniaturizing the antibody incubation box and placing it in a closeable box, the antibody incubation box is guaranteed The stability, the function of the I-shaped hole and the adjustment mechanism make the antibody incubation box can be easily taken out of the box, and the sliding skylight type antibody incubation box is used to effectively solve the problem of exhaust and sealing, and the small experimental system saves reagents such as antibodies The usage amount is small, it is convenient for repeated implementation, and it has wide practicability in the field of medical experiments.
为了解决上述问题，本实用新型采用的技术方案如下：In order to solve the above problems, the technical scheme adopted by the utility model is as follows:
本实用新型提供的一种适用于免疫印迹试验的抗体孵育装置，包括箱体、箱盖和抗体孵育盒；箱体中，箱体相对的两侧设置多组工型孔，箱体内在对应的工型孔间设有调节机构，箱体顶部设置与抗体孵育盒相适应的孔，箱体顶部一侧设置封口槽；抗体孵育盒中，盒体顶端设置凹槽，盒盖底端设置与凹槽相适应的凸起一，盒盖与盒体通过卡槽连接；抗体孵育盒通过孔放置在箱体内，箱体上方设有箱盖，箱盖和箱体通过活动合页连接，箱盖底端设置与封口槽相适应的凸起二。The utility model provides an antibody incubation device suitable for immunoblotting test, which includes a box body, a box cover and an antibody incubation box; in the box body, a plurality of groups of I-shaped holes are arranged on opposite sides of the box body, and corresponding There is an adjustment mechanism between the I-shaped holes, a hole suitable for the antibody incubation box is set on the top of the box, and a sealing groove is set on one side of the top of the box; in the antibody incubation box, a groove is set on the top of the box body, and a groove is set on the bottom of the box cover. The groove is compatible with the protrusion one, and the box cover is connected with the box body through the card slot; the antibody incubation box is placed in the box through the hole, and the box cover is provided on the top of the box body, and the box cover and the box body are connected by a movable hinge, and the bottom of the box cover The end is provided with two protrusions adapted to the sealing groove.
本实用新型中，调节机构包括调节手柄，调节杆和支撑圆柱，调节手柄间通过调节杆活动连接，调节杆上与调节杆上的支撑圆柱固定连接。In the utility model, the adjustment mechanism includes an adjustment handle, an adjustment rod and a support cylinder, the adjustment handles are movably connected through the adjustment rod, and the adjustment rod is fixedly connected with the support cylinder on the adjustment rod.
本实用新型中，调节手柄呈长方体状，调节手柄一侧设置连杆孔，调节杆中，连杆两端设置限制柄头，限制柄头呈圆柱状，限制柄头的直径大于连杆孔的直径，调节杆通过连杆孔与调节手柄活动连接。In the utility model, the adjusting handle is in the shape of a cuboid, and a connecting rod hole is arranged on one side of the adjusting handle. In the adjusting rod, a limiting handle is arranged at both ends of the connecting rod. The limiting handle is cylindrical, and the diameter of the limiting handle is larger than that of the connecting rod hole. Diameter, the adjusting rod is flexibly connected with the adjusting handle through the connecting rod hole.
本实用新型中，调节手柄穿过工型孔与工型孔相适应设置。In the utility model, the adjustment handle passes through the I-shaped hole and is arranged in conformity with the I-shaped hole.
本实用新型中，箱盖顶端靠近凸起二一侧设置抓手端。In the utility model, the top of the box cover is provided with a handle end near the side of the protrusion two.
本实用新型中，抗体孵育盒的尺寸为，长30mm,宽5mm,高2mm。In the utility model, the size of the antibody incubation box is 30mm long, 5mm wide and 2mm high.
本实用新型中，抗体孵育盒的尺寸为，长30mm,宽10mm,高2mm。In the utility model, the size of the antibody incubation box is 30mm long, 10mm wide and 2mm high.
本实用新型中，抗体孵育盒的尺寸为，长30mm,宽2mm,高5mm。In the utility model, the size of the antibody incubation box is 30mm long, 2mm wide and 5mm high.
本实用新型中，抗体孵育盒的尺寸为，长30mm,宽2mm,高10mm。In the utility model, the size of the antibody incubation box is 30mm long, 2mm wide and 10mm high.
使用本实用新型提供的技术方案可获得如下有益效果：Using the technical solution provided by the utility model can obtain the following beneficial effects:
本实用新型提供的一种适用于免疫印迹试验的抗体孵育装置结构简单，使用方便，通过将抗体孵育盒小型化并置于可封闭的箱体中，确保了抗体孵育盒的稳定性，工型孔和调节机构的作用使得抗体孵育盒可以方便地从箱体中取出，采用推拉天窗式的抗体孵育盒有效解决了排气和封闭的问题，实验体系小节省抗体等试剂的使用量，便于多次重复实施，在医疗实验领域具有广泛的实用性。The utility model provides an antibody incubation device suitable for western blotting test, which is simple in structure and easy to use. By miniaturizing the antibody incubation box and placing it in a closeable box, the stability of the antibody incubation box is ensured. The role of the hole and the adjustment mechanism makes the antibody incubation box can be easily taken out of the box. The use of the sliding skylight-type antibody incubation box effectively solves the problem of exhaust and sealing. The small experimental system saves the amount of reagents such as antibodies and is convenient for multiple It can be implemented repeatedly and has wide practicability in the field of medical experiments.
附图说明Description of drawings
图1显示为本实用新型的结构示意图。Fig. 1 shows the structural representation of the utility model.
图2显示为本实用新型提供的调节机构结构示意图。Fig. 2 shows a schematic structural diagram of the adjustment mechanism provided by the present invention.
图3显示为本实用新型提供的调节杆结构示意图。Fig. 3 shows a schematic structural diagram of the adjusting rod provided by the present invention.
图4显示为本实用新型提供的抗体孵育盒结构示意图。Fig. 4 shows a schematic diagram of the structure of the antibody incubation box provided by the present invention.
图5显示为本实用新型提供的抗体孵育装置使用示意图一。Figure 5 shows the first schematic diagram of the use of the antibody incubation device provided by the present invention.
图6显示为本实用新型提供的抗体孵育装置使用示意图二。Figure 6 shows the second schematic diagram for the use of the antibody incubation device provided by the present invention.
在图1-6中：1—箱体，11—调节机构，111—调节手柄，1111—连杆孔，112—调节杆，1121—限制柄头，1122—连杆,113—支撑圆柱，12—工型孔，13—封口槽，2—抗体孵育盒，21—盒盖，211—凸起一，22—盒体，221—凹槽，3—箱盖，31—抓手端，32—凸起二，4—活动合页。In Fig. 1-6: 1—box body, 11—adjusting mechanism, 111—adjusting handle, 1111—connecting rod hole, 112—adjusting rod, 1121—restricting crown, 1122—connecting rod, 113—supporting cylinder, 12 —I-shaped hole, 13—sealing groove, 2—antibody incubation box, 21—box cover, 211—protrusion, 22—box body, 221—groove, 3—box cover, 31—grip end, 32— Protrusion 2, 4-movable hinge.
具体实施方式detailed description
下面结合附图1-6和实施例，对本实用新型的具体实施方式作进一步详细描述，但本实用新型装置不限于下述实施例。Below in conjunction with accompanying drawing 1-6 and embodiment, the specific embodiment of the utility model is described in further detail, but the device of the utility model is not limited to following embodiment.
在本实用新型中，为了便于描述，一种适用于免疫印迹试验的抗体孵育装置中各部件的相对位置关系的描述是根据附图1的布图方式来进行描述的，如：上、下、左、右等位置关系是依据说明书附图1的布图方向来确定的。In this utility model, for the convenience of description, the description of the relative positional relationship of each component in an antibody incubation device suitable for Western blot test is described according to the layout method of accompanying drawing 1, such as: upper, lower, Positional relationships such as left and right are determined according to the layout direction of Figure 1 of the specification.
本实用新型提供的一种适用于免疫印迹试验的抗体孵育装置，包括箱体（1）、箱盖（3）和抗体孵育盒（2）；箱体（1）中，箱体（1）相对的两侧设置多组工型孔（12），箱体（1）内在对应的工型孔（12）间设有调节机构（11），箱体（1）顶部设置与抗体孵育盒（2）相适应的孔，箱体（1）顶部一侧设置封口槽（13）；抗体孵育盒（2）中，盒体（22）顶端设置凹槽（221），盒盖（21）底端设置与凹槽（221）相适应的凸起一（211），盒盖（21）与盒体（22）通过卡槽连接；抗体孵育盒（2）通过孔放置在箱体（1）内，箱体（1）上方设有箱盖（3），箱盖（3）和箱体（1）通过活动合页（4）连接，箱盖（3）底端设置与封口槽（13）相适应的凸起二（32）。The utility model provides an antibody incubation device suitable for immunoblotting test, which includes a box (1), a box cover (3) and an antibody incubation box (2); in the box (1), the box (1) is opposite to Multiple sets of I-shaped holes (12) are set on both sides of the box body (1), and an adjustment mechanism (11) is provided between the corresponding I-shaped holes (12), and the top of the box body (1) is provided with an antibody incubation box (2) For the corresponding holes, a sealing groove (13) is provided on the top side of the box body (1); in the antibody incubation box (2), a groove (221) is set on the top of the box body (22), and a groove (221) is set on the bottom end of the box cover (21). The groove (221) is compatible with the protrusion one (211), and the box cover (21) is connected with the box body (22) through the card slot; the antibody incubation box (2) is placed in the box body (1) through the hole, and the box body (1) There is a box cover (3) on the top, and the box cover (3) and the box body (1) are connected by a movable hinge (4). from two (32).
本实用新型中，调节机构（11）包括调节手柄（111），调节杆（112）和支撑圆柱（113），调节手柄（111）间通过调节杆（112）活动连接，调节杆（112）上与调节杆（112）上的支撑圆柱（113）固定连接。In the utility model, the adjustment mechanism (11) includes an adjustment handle (111), an adjustment rod (112) and a support cylinder (113). It is fixedly connected with the support column (113) on the adjusting rod (112).
本实用新型中，调节手柄（111）呈长方体状，调节手柄（111）一侧设置连杆孔（1111），调节杆（112）中，连杆（1122）两端设置限制柄头（1121），限制柄头（1121）呈圆柱状，限制柄头（1121）的直径大于连杆孔（1111）的直径，调节杆（112）通过连杆孔（1111）与调节手柄（111）活动连接。In the utility model, the adjusting handle (111) is in the shape of a cuboid, and a connecting rod hole (1111) is arranged on one side of the adjusting handle (111), and in the adjusting rod (112), a limiting handle (1121) is arranged at both ends of the connecting rod (1122) , the limiting crown (1121) is cylindrical, the diameter of the limiting crown (1121) is greater than the diameter of the connecting rod hole (1111), and the adjusting rod (112) is movably connected with the adjusting handle (111) through the connecting rod hole (1111).
本实用新型中，调节手柄（111）穿过工型孔（12）与工型孔（12）相适应设置。In the present utility model, the adjusting handle (111) passes through the I-shaped hole (12) and is arranged in conformity with the I-shaped hole (12).
本实用新型中，箱盖（3）顶端靠近凸起二（32）一侧设置抓手端（31）。In the utility model, the top of the case cover (3) is provided with a handle end (31) near the side of the second protrusion (32).
本实用新型中，抗体孵育盒（2）的尺寸为，长30mm,宽5mm,高2mm。In the utility model, the size of the antibody incubation box (2) is 30mm long, 5mm wide and 2mm high.
本实用新型中，抗体孵育盒（2）的尺寸为，长30mm,宽10mm,高2mm。In the utility model, the size of the antibody incubation box (2) is 30mm long, 10mm wide and 2mm high.
本实用新型中，抗体孵育盒（2）的尺寸为，长30mm,宽2mm,高5mm。In the utility model, the size of the antibody incubation box (2) is 30mm long, 2mm wide and 5mm high.
本实用新型中，抗体孵育盒（2）的尺寸为，长30mm,宽2mm,高10mm。In the utility model, the size of the antibody incubation box (2) is 30mm long, 2mm wide and 10mm high.
实验过程中，如上所述尺寸的抗体孵育盒（2）体积小，张力大，液体不容易受振动洒出，实验体系小节省抗体等试剂的使用量，便于多次重复实施。During the experiment, the antibody incubation box (2) of the above-mentioned size has small volume and high tension, and the liquid is not easily spilled by vibration. The small experimental system saves the use of reagents such as antibodies, and is convenient for repeated implementation.
在使用本实用新型提供的一种适用于免疫印迹试验的抗体孵育装置时，通过抓手端（31）打开箱盖，在箱体（1）中放入抗体孵育盒（2），向外拉出调节手柄（111）到工型孔（12）外旋转90°使调节机构（11）从工型孔（12）中向下活动，到工型孔（12）底端后旋转调节手柄（111）90°，压回调节手柄（111）到工型孔（12）中固定，盖上箱体（1）使凸起二（32）扣入封口槽（13）中；需要排气时，打开箱盖（3），采用上述步骤的反顺序将调节手柄（111）移到工型孔（12）的上端，通过镊子推动盒盖（21）排气。When using the antibody incubation device suitable for western blot test provided by the utility model, open the box cover through the handle end (31), put the antibody incubation box (2) in the box body (1), and pull it out Out of the adjustment handle (111) to the I-shaped hole (12) and rotate 90° to make the adjustment mechanism (11) move downward from the I-shaped hole (12), and then rotate the adjustment handle (111) to the bottom of the I-shaped hole (12) ) at 90°, press back the adjusting handle (111) to fix it in the work-shaped hole (12), cover the box (1) so that the second protrusion (32) snaps into the sealing groove (13); when it is necessary to exhaust, open For the box cover (3), use the reverse order of the above steps to move the adjustment handle (111) to the upper end of the I-shaped hole (12), and push the box cover (21) to exhaust air through tweezers.
本实用新型提供的一种适用于免疫印迹试验的抗体孵育装置结构简单，使用方便，通过将抗体孵育盒（2）小型化并置于可封闭的箱体（1）中，确保了抗体孵育盒（2）的稳定性，工型孔（12）和调节机构（11）的作用使得抗体孵育盒（2）可以方便地从箱体（1）中取出，采用推拉天窗式的抗体孵育盒（2）有效解决了排气和封闭的问题，实验体系小节省抗体等试剂的使用量，便于多次重复实施，在医疗实验领域具有广泛的实用性。The utility model provides an antibody incubation device suitable for western blotting test with simple structure and convenient use. By miniaturizing the antibody incubation box (2) and placing it in a closeable box (1), the antibody incubation box is ensured. The stability of (2), the function of the I-shaped hole (12) and the adjustment mechanism (11) make the antibody incubation box (2) can be easily taken out from the box (1), and the antibody incubation box (2 ) effectively solves the problems of exhaust and sealing, the small experimental system saves the use of reagents such as antibodies, and is convenient for repeated implementation, and has wide practicability in the field of medical experiments.
如上所述，即可较好地实现本实用新型,上述的实施例仅仅是对本实用新型的优选实施方式进行描述，并非对本实用新型的范围进行限定，在不脱离本实用新型设计精神的前提下，本领域普通技术人员对本实用新型的技术方案作出的各种变形和改进，均应落入本实用新型确定的保护范围内。As mentioned above, the utility model can be better realized. The above-mentioned embodiment is only a description of the preferred implementation of the utility model, and does not limit the scope of the utility model. Without departing from the design spirit of the utility model, Therefore, various modifications and improvements made by those skilled in the art to the technical solution of the utility model shall fall within the scope of protection determined by the utility model.
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