CN202420935U - Dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank - Google Patents
Dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank Download PDFInfo
- Publication number
- CN202420935U CN202420935U CN2012200354271U CN201220035427U CN202420935U CN 202420935 U CN202420935 U CN 202420935U CN 2012200354271 U CN2012200354271 U CN 2012200354271U CN 201220035427 U CN201220035427 U CN 201220035427U CN 202420935 U CN202420935 U CN 202420935U
- Authority
- CN
- China
- Prior art keywords
- dual
- polyacrylamide gel
- baffle plate
- gel electrophoresis
- transverse
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000004043 dyeing Methods 0.000 title claims abstract description 22
- 238000002264 polyacrylamide gel electrophoresis Methods 0.000 title claims abstract description 12
- 210000005056 cell body Anatomy 0.000 claims description 14
- 239000003153 chemical reaction reagent Substances 0.000 abstract description 4
- 239000002699 waste material Substances 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract 1
- 238000001962 electrophoresis Methods 0.000 description 17
- 238000004042 decolorization Methods 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 206010053567 Coagulopathies Diseases 0.000 description 2
- 230000035602 clotting Effects 0.000 description 2
- 229920002401 polyacrylamide Polymers 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 108010044467 Isoenzymes Proteins 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000005251 capillar electrophoresis Methods 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 238000001155 isoelectric focusing Methods 0.000 description 1
- 239000006101 laboratory sample Substances 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
The utility model relates to an experimental instrument, in particular to a dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank. The dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank consists of a tank body, a transverse baffle plate and a vertical baffle plate, wherein the top end of the tank body is opened; a plurality of pinholes are distributed on a bottom panel; longitudinal slots are uniformly distributed on the inner wall surfaces of the two parallel lateral panels of the tank body; a transverse through slot is formed in the center of the rest two lateral panels; the vertical baffle plate passes through the longitudinal slots and is detachably connected with the longitudinal slots; the transverse through slot passes through the lateral panels; the transverse baffle plate passes through the transverse through slot and is detachably connected with the transverse through slot; one end of the transverse baffle plate is provided with a bulge; and the top end of the tank body is provided with two mutually symmetrical handles. The dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank overcomes the defects that the conventional container easily wastes reagent, has single function, is difficult to operate and mark and the like, and has certain advantages.
Description
Technical field
The utility model relates to a kind of experimental apparatus, especially a kind of dual-purpose polyacrylamide gel electrophoresis dyeing decolouring groove.
Background technology
Electrophoretic techniques is the experimental technique of field widespread uses such as biological chemistry, molecular biology; Various ways is arranged: vertical slab electrophoresis, disc electrophoresis, horizontal strip electrophoresis, Capillary Electrophoresis etc.; Vertical slab electrophoresis commonly used and disc electrophoresis in scientific experiment; Majority as separating medium, is suitable for multiple bioactivators such as isolated protein, amino acid, nucleic acid with polyacrylamide gel.The electrophoresis operating process is complicated, length consuming time, and wherein dyeing and decolorization are one of committed steps that can experiment successful.According to the difference of laboratory sample character, dyeing is different with the decolouring agents useful for same, but dyeing, decolorization is basic identical: the gel strip that will have different samples is separated; Immerse dyeing liquor respectively; The dyeing back that finishes is taken out with instrument again and is cleaned with distilled water, immerses respectively then and repeatedly changes destainer to colour developing in the destainer fully.
Do not have as yet at present and be applicable to the industrial products that dye and decolour behind the electrophoresis; The normal dyeing of each scientific experiment chamber, decolouring are to adopt the double dish that is fit to size; What of quantity per sample behind the disc electrophoresis; Each double dish is placed 2～3 gel strip, for preventing that the gel strip in each double dish is mixed up, generally forbids arbitrarily moving; Two clotting films are positioned over dyeing and decolouring in two cultivations respectively behind the vertical slab electrophoresis.
The shortcoming of prior art is: each glass tube sample of disc electrophoresis is different, needs with the separately dyeing of different containers, waste reagent; The general electrophoresis simultaneously of vertical slab electrophoresis goes out two clotting glue, also will separately dye, and especially does isozyme electrophoresis, because substrate costs an arm and a leg, can cause unnecessary waste; When doing the isoelectric focusing disc electrophoresis, need to distinguish the two poles of the earth up and down of gel, be difficult to separately when common dyeing and decolouring, can only use the test of pH test paper; At present medium majority of electrophoresis be polyacrylamide gel, and this gel is soft flexible, still dye and decolorization in constantly change liquid because of needs, with tweezers repeatedly clamping be easy to damage colloid.
The utility model content
The utility model is intended to address the above problem, and a kind of dual-purpose polyacrylamide gel electrophoresis dyeing decolouring groove is provided, and it has overcome uses existing container to be prone to waste reagent, function singleness, troublesome poeration, is difficult for drawback such as mark, and its technical scheme that adopts is following:
A kind of dual-purpose polyacrylamide gel electrophoresis dyeing decolouring groove; Be made up of cell body, lagging and perpendicular baffle plate, said cell body top end opening is placed with several apertures on the bottom panel; Be evenly equipped with cannelure on the internal face of two side panels that parallel of said cell body; Panels middle part in remaining both sides has a horizontal groove, and said perpendicular baffle plate is threaded onto in the cannelure, and with cannelure be detachable connection; Said horizontal groove runs through side panel, said lagging be threaded onto in the horizontal groove and with horizontal groove be detachable connection.
On the technique scheme basis, an end of said lagging has a convexity.
On the technique scheme basis, the top of said cell body is provided with two symmetrical handles.
The utlity model has following advantage: have dual-use function, not only be applicable to vertical slab electrophoresis but also be applicable to disc electrophoresis, can be used for dyeing and can accomplish decolorization simultaneously again; Save reagent, not fragile gel, save time, easy to operate, be suitable for mark, applied widely.
Description of drawings
Fig. 1: the utility model face the blast structural representation;
Fig. 2: the utility model overlook the blast structural representation;
Fig. 3: the side-looking structural representation of the said lagging of the utility model;
Symbol description:
1. cell body, 2. lagging, 3. perpendicular baffle plate, 4. aperture, 5. cannelure, 6. laterally groove, 7. handle, 8. convexity.
Embodiment
Below in conjunction with accompanying drawing and instance the utility model is described further:
Extremely shown in Figure 3 like Fig. 1; A kind of dual-purpose polyacrylamide gel electrophoresis dyeing decolouring groove of present embodiment is made up of said cell body 1 top end opening cell body 1, lagging 2 and perpendicular baffle plate 3; Be placed with several apertures 4 on the bottom panel; 4 infiltrations of said aperture, ooze out solution usefulness, be evenly equipped with cannelure 5 on the internal face of two side panels that parallel of said cell body 1, panel middle part in remaining both sides has a horizontal groove 6; Said perpendicular baffle plate 3 is threaded onto in the cannelure 5, and is detachable connection with cannelure 5; Said horizontal groove 6 runs through side panel, and said lagging 2 is threaded onto in the horizontal groove 6 and with horizontal groove 6 and is detachable connection.
Preferably, an end of said lagging 2 has a convexity 8, and protruding 8 can be convenient to when grafting lagging 2, position.
Preferably, the top of said cell body 1 is provided with two symmetrical handles 7, and handle 7 can person easy to use grip mobile cell body 1 here.
With way of example the utility model is illustrated above, but the utility model is not limited to above-mentioned specific embodiment, all any changes done based on the utility model or modification all belong to the utility model and require the scope protected.
Claims (3)
1. dual-purpose polyacrylamide gel electrophoresis dyeing decolouring groove; It is characterized in that: be made up of cell body (1), lagging (2) and perpendicular baffle plate (3), said cell body (1) top end opening is placed with several apertures (4) on the bottom panel; Be evenly equipped with cannelure (5) on the internal face of two side panels that parallel of said cell body (1); Panels middle part in remaining both sides has a horizontal groove (6), and said perpendicular baffle plate (3) is threaded onto in the cannelure (5), and with cannelure (5) be detachable connection; Said horizontal groove (6) runs through side panel, and said lagging (2) is threaded onto in the horizontal groove (6) and with horizontal groove (6) and is detachable connection.
2. a kind of dual-purpose polyacrylamide gel electrophoresis dyeing decolouring groove according to claim 1, it is characterized in that: an end of said lagging (2) has a convexity (8).
3. a kind of dual-purpose polyacrylamide gel electrophoresis dyeing decolouring groove according to claim 1, it is characterized in that: the top of said cell body (1) is provided with two symmetrical handles (7).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200354271U CN202420935U (en) | 2012-01-13 | 2012-01-13 | Dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2012200354271U CN202420935U (en) | 2012-01-13 | 2012-01-13 | Dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202420935U true CN202420935U (en) | 2012-09-05 |
Family
ID=46745689
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2012200354271U Expired - Fee Related CN202420935U (en) | 2012-01-13 | 2012-01-13 | Dual-purpose polyacrylamide gel electrophoresis dyeing and decolorizing tank |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202420935U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102944460A (en) * | 2012-11-27 | 2013-02-27 | 齐齐哈尔大学 | Gel silver dyeing box for gel test |
| CN106053188A (en) * | 2016-06-24 | 2016-10-26 | 广东医学院 | Gel dyeing-decoloration integrated equipment and control method thereof |
| CN108871914A (en) * | 2018-09-10 | 2018-11-23 | 河北省农林科学院粮油作物研究所 | A kind of post-processing approach and device of polyacrylamide gel electrophoresis product |
-
2012
- 2012-01-13 CN CN2012200354271U patent/CN202420935U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102944460A (en) * | 2012-11-27 | 2013-02-27 | 齐齐哈尔大学 | Gel silver dyeing box for gel test |
| CN106053188A (en) * | 2016-06-24 | 2016-10-26 | 广东医学院 | Gel dyeing-decoloration integrated equipment and control method thereof |
| CN106053188B (en) * | 2016-06-24 | 2019-01-29 | 广东医科大学 | A kind of gel dye decoloration integration apparatus and its control method |
| CN108871914A (en) * | 2018-09-10 | 2018-11-23 | 河北省农林科学院粮油作物研究所 | A kind of post-processing approach and device of polyacrylamide gel electrophoresis product |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120905 Termination date: 20130113 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |