CN206089708U - Simple and easy cell pressurization culture apparatus - Google Patents

Abstract

The utility model provides a simple pressurized culture device for cells, which is characterized in that it comprises a three-way pipe, and the first port, the second port and the third port of the three-way pipe are respectively connected with a culture bottle, a pressurized ball and a pressure gauge. The device of the utility model is easy to manufacture, the pressure is controllable and the pressurization effect is good.

Description

A Simple Cell Pressurized Culture Device

technical field

The utility model relates to a simple cell pressurized culture device, which is used for in vitro pressurized culture of cells.

Background technique

Glaucoma is a group of ocular diseases characterized by progressive loss of retinal ganglion cells and their axons, and progressive loss of visual field. One of the factors that contribute to the disease is increased pressure in the eye. For glaucoma research, it is necessary to establish an in vitro glaucoma ocular hypertension model, and the existing in vitro glaucoma ocular hypertension model cannot control the pressure.

Contents of the invention

The purpose of the utility model is to provide a simple cell pressurized culture device which can control the pressure value acting on the cells.

In order to achieve the above object, the utility model provides a simple cell pressurized culture device, which is characterized in that it includes a three-way pipe, the first port, the second port and the third port of the three-way pipe are respectively connected to the culture bottle, pressurized Ball and pressure gauge.

Preferably, the mouth of the culture bottle is provided with a rubber stopper for sealing, and the first port of the three-way pipe passes through the rubber stopper.

Preferably, the second port and the third port of the tee are respectively connected to a pressurized ball and a pressure gauge through a rubber tube.

More preferably, the simple pressurized cell culture device further includes a clamp capable of clamping the rubber tube.

Compared with the prior art, the beneficial effects of the utility model are:

The device of the utility model is easy to manufacture, the pressure is controllable and the pressurization effect is good.

Description of drawings

Figure 1 is a diagram of a simple cell pressurized culture device.

detailed description

Below in conjunction with specific embodiment, further set forth the utility model. It should be understood that these embodiments are only used to illustrate the present utility model and are not intended to limit the scope of the present utility model. In addition, it should be understood that after reading the content taught by the utility model, those skilled in the art can make various changes or modifications to the utility model, and these equivalent forms also fall within the scope defined by the appended claims of the application.

Example

As shown in Figure 1, it is a simple cell pressurized culture device, and the simple cell pressurized culture device includes a three-way pipe 1, and the first port, the second port and the third port of the three-way pipe 1 are respectively connected to the culture bottle 2 , Pressurization ball 3 and pressure gauge 4. The mouth of the culture bottle 2 is provided with a rubber stopper for sealing, and the first port of the three-way pipe 1 passes through the rubber stopper. The second port and the third port of the three-way pipe 1 are respectively connected to the pressurized ball 3 and the pressure gauge 4 through rubber tubes. The simple cell pressurized culture device also includes a clip capable of clamping the rubber tube.

The capacity of described culture bottle 2 is 25ml, and pressurization ball 3 can be taken from sphygmomanometer, is used for pressurizing; The specific pressure value; Tee pipe 1 and rubber stopper and rubber tube are customized according to the caliber of culture bottle 2 and the caliber of pressurized ball 3 and pressure gauge 4 connection ports.

During installation, one end of the tee pipe 1 is inserted in the middle hole of the rubber stopper, and the rubber stopper is passed through. Connect the other two ends of the three-way pipe 1 to the pressure ball 3 and the pressure gauge 4 with rubber tubes respectively. Finally, stopper the culture bottle 2 with the rubber stopper, and the simple cell pressurized culture device has just been installed.

During use, pump air into the culture bottle 2 with the pressurized ball 3, and simultaneously use the pressure gauge 4 to detect the pressure in the bottle. When the pressure reaches the desired pressure value, clamp the rubber tube on one side of the pressure ball 3 with a clamp, and finally clamp the rubber tube on the pressure gauge 4 side.

Claims (4)

1. a kind of Simple cell pressurizes culture apparatuses, it is characterised in that including tee T (1), the first port of tee T (1), Second port and the 3rd port connect respectively culture bottle (2), pressurizing ball (3) and pressure gauge (4).

2. Simple cell as claimed in claim 1 pressurizes culture apparatuses, it is characterised in that the bottleneck of described culture bottle (2) The rubber closure of sealing is provided with, the first port of tee T (1) passes through rubber closure.

3. Simple cell as claimed in claim 1 pressurizes culture apparatuses, it is characterised in that the second of described tee T (1) Port and the 3rd port connect pressurizing ball (3) and pressure gauge (4) by rubber tube respectively.

4. Simple cell as claimed in claim 3 pressurizes culture apparatuses, it is characterised in that described Simple cell pressurization culture Device also includes the clip that can be closed rubber pipe clamp.