CN205473826U - A open pressure control cell culture system for glaucoma basic research - Google Patents
A open pressure control cell culture system for glaucoma basic research Download PDFInfo
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- CN205473826U CN205473826U CN201620201377.8U CN201620201377U CN205473826U CN 205473826 U CN205473826 U CN 205473826U CN 201620201377 U CN201620201377 U CN 201620201377U CN 205473826 U CN205473826 U CN 205473826U
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- 208000010412 Glaucoma Diseases 0.000 title claims abstract description 15
- 238000004113 cell culture Methods 0.000 title claims abstract description 12
- 241001411320 Eriogonum inflatum Species 0.000 claims abstract description 20
- 239000000523 sample Substances 0.000 claims description 3
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 abstract description 58
- 229910002092 carbon dioxide Inorganic materials 0.000 abstract description 29
- 239000001569 carbon dioxide Substances 0.000 abstract description 29
- 239000007789 gas Substances 0.000 description 8
- 210000004027 cell Anatomy 0.000 description 2
- 238000012136 culture method Methods 0.000 description 2
- 230000004406 elevated intraocular pressure Effects 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000004382 visual function Effects 0.000 description 2
- 206010030043 Ocular hypertension Diseases 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000006735 deficit Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002919 epithelial cell Anatomy 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000011173 large scale experimental method Methods 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 210000003994 retinal ganglion cell Anatomy 0.000 description 1
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Abstract
本实用新型涉及用于青光眼基础研究的开放式压力控制细胞培养系统，其包括二氧化碳培养箱、培养瓶、压力表、流量计、气泵、变频器、流量阀、瓶塞、控制系统；培养瓶、压力表、流量计、气泵、变频器、流量阀、瓶塞均设置于二氧化碳培养箱内。本实用新型的气泵的入口端、流量阀的出口端均在二氧化碳培养箱，保证了培养瓶与二氧化碳培养箱之间的气体交换，实验条件与活体条件比较接近；本实用新型的控制系统设置于二氧化碳培养箱外，在二氧化碳培养箱外就可以操作本实用新型，操作方便。本实用新型的气泵一般情况下不会处于负荷运转状态，因此比较节能。
The utility model relates to an open pressure control cell culture system used for basic research on glaucoma, which comprises a carbon dioxide incubator, a culture bottle, a pressure gauge, a flow meter, an air pump, a frequency converter, a flow valve, a bottle stopper and a control system; the culture bottle, Pressure gauges, flow meters, air pumps, frequency converters, flow valves, and bottle stoppers are all set in the carbon dioxide incubator. The inlet end of the air pump and the outlet end of the flow valve of the utility model are all in the carbon dioxide incubator, which ensures the gas exchange between the culture bottle and the carbon dioxide incubator, and the experimental conditions are relatively close to the conditions of the living body; the control system of the utility model is arranged in Outside the carbon dioxide incubator, the utility model can be operated outside the carbon dioxide incubator, and the operation is convenient. Generally speaking, the air pump of the utility model will not be in the load operation state, so it is more energy-saving.
Description
技术领域technical field
本实用新型涉及细胞培养系统，具体涉及用于青光眼基础研究的开放式压力控制细胞培养系统。The utility model relates to a cell culture system, in particular to an open pressure control cell culture system used for glaucoma basic research.
背景技术Background technique
眼压升高是青光眼的特征之一，也是导致青光眼视功能损害的主要因素。相关研究已经证实：眼压升高、波动或持续升高，可以导致视网膜神经节细胞、色素上皮细胞等的功能损害或死亡，最终导致视功能的丧失。Elevated intraocular pressure is one of the characteristics of glaucoma, and it is also the main factor leading to the impairment of visual function in glaucoma. Relevant studies have confirmed that: elevated intraocular pressure, fluctuating or continuous elevation can lead to functional damage or death of retinal ganglion cells, pigment epithelial cells, etc., and eventually lead to loss of visual function.
青光眼研究模型可分为活体青光眼动物模型和离体青光眼模型，后者又包括体外高眼压灌注模型和细胞培养加压模型。细胞培养加压模型作为细胞水平的青光眼研究模型，其突出优点是:能够自由设定压力水平，能够获得恒定压力，能够排除混杂因素的影响，而且容易进行大通量实验。Glaucoma research models can be divided into in vivo glaucoma animal models and isolated glaucoma models, which in turn include in vitro ocular hypertension perfusion models and cell culture pressurized models. As a cell-level glaucoma research model, the cell culture pressurized model has the following advantages: it can freely set the pressure level, obtain a constant pressure, eliminate the influence of confounding factors, and is easy to carry out large-scale experiments.
但传统的密闭培养法是向培养瓶中持续注入高压气体，隔绝了培养瓶与培养箱中气体的交换，随着实验时间的延长可能会引起培养液pH值、HCO- 3浓度、氧气分压(pO2)、二氧化碳分压(pCO2)的变化，从而影响细胞的生理功能，实验条件与活体条件的差别较大。However, the traditional airtight culture method is to continuously inject high-pressure gas into the culture bottle, which cuts off the gas exchange between the culture bottle and the incubator. With the prolongation of the experiment time, the pH value of the culture solution, the concentration of HCO - 3 , and the partial pressure of oxygen may be caused. (pO 2 ) and carbon dioxide partial pressure (pCO 2 ) changes, thereby affecting the physiological functions of cells, and the experimental conditions are quite different from the living conditions.
另外，传统的密闭培养法是向培养瓶中持续注入高压气体，气泵持续处于满负荷运转的状态，不利于节能。In addition, the traditional airtight culture method is to continuously inject high-pressure gas into the culture bottle, and the air pump is continuously running at full capacity, which is not conducive to energy saving.
实用新型内容Utility model content
为解决上述问题，本实用新型的目的在于提供实验条件接近活体条件、节能且使用方便的用于青光眼基础研究的开放式压力控制细胞培养系统。In order to solve the above problems, the purpose of this utility model is to provide an open pressure control cell culture system for basic research on glaucoma with experimental conditions close to living conditions, energy saving and easy to use.
为实现上述目的，本实用新型采取的技术方案为：用于青光眼基础研究的开放式压力控制细胞培养系统，其包括二氧化碳培养箱、培养瓶、压力表、流量计、气泵、变频器、流量阀、瓶塞、控制系统；培养瓶、压力表、流量计、气泵、变频器、流量阀、瓶塞均设置于二氧化碳培养箱内；气泵的出口端与流量计的入口端通过管道连接；瓶塞设置于培养瓶瓶口；流量计的出口端通过管道穿过瓶塞插入培养瓶内；流量阀的入口端通过管道穿过瓶塞插入培养瓶内；压力表的探头穿过瓶塞插入培养瓶内；气泵的入口端、流量阀的出口端均设置于二氧化碳培养箱内；气泵与变频器连接；压力表、流量计、变频器、流量阀分别通过信号线与控制系统相连；控制系统设置于二氧化碳培养箱外。In order to achieve the above object, the technical solution adopted by the utility model is: an open pressure control cell culture system for glaucoma basic research, which includes a carbon dioxide incubator, a culture bottle, a pressure gauge, a flow meter, an air pump, a frequency converter, and a flow valve , bottle stopper, and control system; culture bottles, pressure gauges, flow meters, air pumps, frequency converters, flow valves, and bottle stoppers are all set in the carbon dioxide incubator; the outlet end of the air pump is connected to the inlet end of the flow meter through pipelines; the bottle stopper Set at the mouth of the culture bottle; the outlet end of the flowmeter is inserted into the culture bottle through the bottle stopper through the pipe; the inlet end of the flow valve is inserted into the culture bottle through the bottle stopper through the pipe; the probe of the pressure gauge is inserted into the culture bottle through the bottle stopper Inside; the inlet end of the air pump and the outlet end of the flow valve are all set in the carbon dioxide incubator; the air pump is connected to the frequency converter; the pressure gauge, flow meter, frequency converter, and flow valve are respectively connected to the control system through signal lines; the control system is set in outside the carbon dioxide incubator.
进一步地，二氧化碳培养箱内设置有与控制系统通过信号线连接的温度计、加热装置，从而可以使二氧化碳培养箱内保持设定的恒温条件，实验条件与活体条件比较接近。Furthermore, the carbon dioxide incubator is provided with a thermometer and a heating device connected to the control system through a signal line, so that the set constant temperature conditions can be maintained in the carbon dioxide incubator, and the experimental conditions are relatively close to the living conditions.
由于气泵的入口端、流量阀的出口端均在二氧化碳培养箱，保证了培养瓶与二氧化碳培养箱之间的气体交换，实验条件与活体条件比较接近。Since the inlet port of the air pump and the outlet port of the flow valve are both in the carbon dioxide incubator, the gas exchange between the culture bottle and the carbon dioxide incubator is ensured, and the experimental conditions are relatively close to those of the living body.
由于控制系统设置于二氧化碳培养箱外，在二氧化碳培养箱外就可以操作该本实用新型，操作方便。Because the control system is arranged outside the carbon dioxide incubator, the utility model can be operated outside the carbon dioxide incubator, and the operation is convenient.
本实用新型的有益效果：由于气泵一般情况下不会处于负荷运转状态，因此比较节能。Beneficial effects of the utility model: generally, the air pump is not in a load operation state, so it is relatively energy-saving.
附图说明Description of drawings
图1为本实用新型的用于青光眼基础研究的开放式压力控制细胞培养系统的结构示意图。Fig. 1 is a structural schematic diagram of an open pressure-controlled cell culture system for glaucoma basic research according to the present invention.
1、二氧化碳培养箱；2、培养瓶；3、压力表；4、流量计；5、气泵；6、变频器；7、流量阀；8、瓶塞；9、控制系统。1. Carbon dioxide incubator; 2. Culture bottle; 3. Pressure gauge; 4. Flow meter; 5. Air pump; 6. Frequency converter; 7. Flow valve; 8. Bottle stopper; 9. Control system.
具体实施方式detailed description
请参阅图1，本实用新型为用于青光眼基础研究的开放式压力控制细胞培养系统，其包括：二氧化碳培养箱1、培养瓶2、压力表3、流量计4、气泵5、变频器6、流量阀7、瓶塞8、控制系统9。Please refer to Fig. 1, the utility model is an open pressure control cell culture system for glaucoma basic research, which includes: carbon dioxide incubator 1, culture bottle 2, pressure gauge 3, flow meter 4, air pump 5, frequency converter 6, Flow valve 7, bottle stopper 8, control system 9.
培养瓶2、压力表3、流量计4、气泵5、变频器6、流量阀7、瓶塞8均设置于二氧化碳培养箱1内。气泵5的出口端与流量计4的入口端通过管道连接；瓶塞8设置于培养瓶2瓶口；流量计4的出口端通过管道穿过瓶塞8插入培养瓶2内；流量阀7的入口端通过管道穿过瓶塞8插入培养瓶2内；压力表3的探头穿过瓶塞8插入培养瓶2内；气泵5的入口端、流量阀7的出口端均设置于二氧化碳培养箱1内。The culture bottle 2, the pressure gauge 3, the flow meter 4, the air pump 5, the frequency converter 6, the flow valve 7, and the bottle stopper 8 are all arranged in the carbon dioxide incubator 1. The outlet end of air pump 5 is connected with the inlet end of flow meter 4 by pipeline; Bottle stopper 8 is arranged on 2 bottlenecks of culture bottle; The inlet end is inserted into the culture bottle 2 through the bottle stopper 8 through the pipeline; the probe of the pressure gauge 3 is inserted into the culture bottle 2 through the bottle stopper 8; the inlet end of the air pump 5 and the outlet end of the flow valve 7 are all set in the carbon dioxide incubator 1 Inside.
气泵5与变频器6连接，通过变频器6可以调节气泵5的转速从而调节气泵5的流量。The air pump 5 is connected with the frequency converter 6, and the speed of the air pump 5 can be adjusted through the frequency converter 6 so as to adjust the flow rate of the air pump 5.
压力表3、流量计4、变频器6、流量阀7分别通过信号线与控制系统9相连；控制系统9设置于二氧化碳培养箱1外。The pressure gauge 3 , the flow meter 4 , the frequency converter 6 , and the flow valve 7 are respectively connected to the control system 9 through signal lines; the control system 9 is set outside the carbon dioxide incubator 1 .
使用本实用新型时，打开控制系统9，通过控制系统9控制变频器6从而控制气泵5开始运转，气体从气泵5的入口端进入并依次通过、流量计4、培养瓶2、流量阀7并从流量阀7的出口端流出；使培养瓶2与二氧化碳培养箱1进行气体交换。When using the utility model, the control system 9 is opened, and the frequency converter 6 is controlled by the control system 9 to control the air pump 5 to start running. Flow out from the outlet end of the flow valve 7; make the culture bottle 2 and the carbon dioxide incubator 1 carry out gas exchange.
本实用新型开始使用后，控制系统9中显示压力表3所探测的压力值、流量阀7的所设定的流量值、流量计4所探测的流量值。通过控制系统9控制变频器6从而控制气泵5的运转(除非所需的培养瓶2内的气压非常大，需要气泵5满负荷预转，一般情况下气泵5不会处于满负荷运转状态)，配合读取流量计4所探测的流量值，可以控制通过流量计4的流量；通过控制系统9可控制通过流量阀7的流量。根据需要，分别控制通过流量计4流量、通过流量阀7的流量，可以调节培养瓶2内的气压(根据帕斯卡定律,开放式容器内的压力与入口气体压力、流量呈正比,与出口气体压力、流量呈反比)，即培养瓶2内的气压可通过控制系统9控制。After the utility model starts to use, the pressure value detected by the pressure gauge 3, the set flow value of the flow valve 7, and the flow value detected by the flowmeter 4 are displayed in the control system 9. Control the frequency converter 6 through the control system 9 to control the operation of the air pump 5 (unless the air pressure in the required culture bottle 2 is very large, the air pump 5 needs to be pre-rotated at full load, and the air pump 5 will not be in a full-load operation state under normal circumstances), Cooperating with reading the flow value detected by the flowmeter 4, the flow through the flowmeter 4 can be controlled; the flow through the flow valve 7 can be controlled through the control system 9. According to needs, the air pressure in the culture bottle 2 can be adjusted by controlling the flow through the flow meter 4 and the flow through the flow valve 7 respectively (according to Pascal's law, the pressure in the open container is proportional to the inlet gas pressure and flow rate, and is proportional to the outlet gas pressure. , flow is inversely proportional), that is, the air pressure in the culture bottle 2 can be controlled by the control system 9.
由于气泵5的入口端、流量阀7的出口端均在二氧化碳培养箱1，保证了培养瓶2与二氧化碳培养箱1之间的气体交换，实验条件与活体条件比较接近。Since the inlet port of the air pump 5 and the outlet port of the flow valve 7 are both in the carbon dioxide incubator 1, the gas exchange between the culture bottle 2 and the carbon dioxide incubator 1 is ensured, and the experimental conditions are relatively close to the living conditions.
由于控制系统9设置于二氧化碳培养箱1外，在二氧化碳培养箱1外就可以操作该本实用新型，操作方便。Because the control system 9 is arranged outside the carbon dioxide incubator 1, the utility model can be operated outside the carbon dioxide incubator 1, and the operation is convenient.
由于气泵5一般情况下不会处于负荷运转状态，比较节能。Since the air pump 5 is generally not in a load operation state, it is more energy-saving.
进一步的，还可以在二氧化碳培养箱1内设置有与控制系统9通过信号线连接的温度计、加热装置，从而可以使二氧化碳培养箱1内保持设定的恒温条件，实验条件与活体条件比较接近。Further, the carbon dioxide incubator 1 can also be provided with a thermometer and a heating device connected to the control system 9 through a signal line, so that the set constant temperature conditions can be maintained in the carbon dioxide incubator 1, and the experimental conditions are relatively close to the living conditions.
以上的具体实施方式仅为本创作的较佳实施例，并不用以限制本创作，凡在本创作的精神及原则之内所做的任何修改、等同替换、改进等，均应包含在本创作的保护范围之内。The specific implementation above is only a preferred embodiment of this creation, and is not intended to limit this creation. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of this creation should be included in this creation. within the scope of protection.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN112877214A (en) * | 2021-04-07 | 2021-06-01 | 中南大学湘雅医院 | Pressure controlled cell culture device for glaucoma research |
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| CN112877214A (en) * | 2021-04-07 | 2021-06-01 | 中南大学湘雅医院 | Pressure controlled cell culture device for glaucoma research |
| CN112877214B (en) * | 2021-04-07 | 2023-04-25 | 中南大学湘雅医院 | Pressure-controlled cell culture device for glaucoma research |
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