CN205695091U - A kind of convenient device observed and raise planthopper - Google Patents
A kind of convenient device observed and raise planthopper Download PDFInfo
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- CN205695091U CN205695091U CN201620193895.XU CN201620193895U CN205695091U CN 205695091 U CN205695091 U CN 205695091U CN 201620193895 U CN201620193895 U CN 201620193895U CN 205695091 U CN205695091 U CN 205695091U
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- planthopper
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- sponge
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- 241001498622 Cixius wagneri Species 0.000 title claims abstract description 38
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 48
- 235000009566 rice Nutrition 0.000 claims abstract description 44
- 235000016709 nutrition Nutrition 0.000 claims abstract description 28
- 229920001821 foam rubber Polymers 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 240000007594 Oryza sativa Species 0.000 claims abstract description 6
- 230000035764 nutrition Effects 0.000 claims abstract description 6
- 239000000463 material Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims 1
- 102000002322 Egg Proteins Human genes 0.000 abstract description 11
- 108010000912 Egg Proteins Proteins 0.000 abstract description 11
- 210000004681 ovum Anatomy 0.000 abstract description 11
- 241000209094 Oryza Species 0.000 description 42
- 238000000034 method Methods 0.000 description 25
- 235000013601 eggs Nutrition 0.000 description 17
- 210000001672 ovary Anatomy 0.000 description 14
- 238000012360 testing method Methods 0.000 description 14
- 241000176086 Sogatella furcifera Species 0.000 description 12
- 238000011161 development Methods 0.000 description 10
- 230000018109 developmental process Effects 0.000 description 10
- 238000011160 research Methods 0.000 description 10
- 230000012010 growth Effects 0.000 description 7
- 241000196324 Embryophyta Species 0.000 description 6
- 235000013339 cereals Nutrition 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 241000238631 Hexapoda Species 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 230000009286 beneficial effect Effects 0.000 description 3
- 230000006408 female gonad development Effects 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- 239000003595 mist Substances 0.000 description 3
- 235000015097 nutrients Nutrition 0.000 description 3
- 241000008892 Cnaphalocrocis patnalis Species 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 238000011835 investigation Methods 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 description 1
- 244000058871 Echinochloa crus-galli Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000003679 aging effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000001110 calcium chloride Substances 0.000 description 1
- 229910001628 calcium chloride Inorganic materials 0.000 description 1
- 230000027326 copulation Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229910052564 epsomite Inorganic materials 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 235000011389 fruit/vegetable juice Nutrition 0.000 description 1
- 230000012447 hatching Effects 0.000 description 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 210000003101 oviduct Anatomy 0.000 description 1
- 230000017448 oviposition Effects 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000029264 phototaxis Effects 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
- 229910000162 sodium phosphate Inorganic materials 0.000 description 1
- 238000012549 training Methods 0.000 description 1
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- Investigating Or Analysing Biological Materials (AREA)
Abstract
This utility model provides a kind of convenient offer one device studying planthopper, and this device is observed container by top and reservoir forms, and described observation container is surrounded cone by sidewall, has upper and lower two openings, and volume is the inner space of 500 milliliters；One reservoir is surrounded by bottom and sidewall and has an opening；Observing container under shed 41 and be positioned at reservoir not in contact with nutritional solution, the observation container on top combines removably with reservoir, wherein equipped with the nutritional solution of 10 milliliters in reservoir；Get out the circular foam-rubber cushion that the upper and lower part opening thickness 1cm beyond the Great Wall of container is observed on top in advance, the sponge of lower openings is transplanted the rice seedlings that seedling age is 15 days, and allows root pass sponge and to contact with bottom reservoir Middle nutrition liquid.Use such device can significantly improve the life-span of planthopper adult, it is possible to obtain to grow consistent female worm's ovum nest and greater number of planthopper worm's ovum.
Description
Technical field
This utility model belongs to agriculture plant protection field, more specifically, belongs to the Oryza sativa L. evils such as research planthopper
The apparatus and method of worm or produce planthopper ovum for a large amount of.
Background technology
Planthopper has 2 kinds of common types, respectively brown paddy plant hopper, white backed planthopper.White backed planthopper is before paddy growth
Mid-term occurs, and occurs around with secondary rice leaf roller, available while preventing and treating rice leaf roller
Control.And some time causes at paddy growth middle and late stage, and to cause harm the heaviest be brown paddy plant hopper.This worm has cluster to cause harm
Habit, adult, larva suck rice clump bottom juice, and yellow leaf dries up.Adult phototaxis is strong, and happiness is tender
Lay eggs with in rice clump bottom sheath and tender stem tissue in the barnyard grass that green pad in shoe is wet.One female worm can lay eggs 200～
300.Foodstuff is abundant, field humidity is relatively big, temperature 25～when 30 DEG C, is conducive to laying eggs, hatching and larva
Survive.Insect pest is many in a lamellar phenomenon when occurring, and first causes harm in bottom, quickly population outbreak.
Planthopper is gathered at Culm of Rice and takes food harm, when life habit is observed and investigates experimental data, often
Rule method is to live rice seedling and planthopper in worm is placed on big glass tubing in observing, owing to permeability is poor, and pipe endogenous dampness
Degree is very big, and rice seedling easily rots mouldy, and the mist pearl on tube wall can affect observation, if desired on investigation rice seedling
Planthopper quantity time, it is difficult to accurately calculate, bigger error can be brought to experimental data, affect experimental data
Accuracy.This is accomplished by providing a kind of new device observe planthopper and investigate experimental data, carries
The accuracy of high experiment, can also produce the planthopper ovum for experiment or the growth of adult simultaneously in a large number.
Utility model content
This utility model solves problem above, it is provided that a kind of device for cultivating planthopper, and this device includes:
This utility model provides a kind of convenient offer one device studying planthopper, this device by top observe container and
Reservoir forms, and described observation container is surrounded cone by sidewall, has upper and lower two openings, and volume is
The inner space of 500 milliliters；One reservoir is surrounded by bottom and sidewall and has an opening；Observe container
Under shed 41 is positioned at reservoir not in contact with nutritional solution, the observation container on top and reservoir with
Dismountable mode combines, wherein equipped with the nutritional solution of 10 milliliters in reservoir；On being ready in advance
The circular foam-rubber cushion of the upper and lower part opening thickness 1cm beyond the Great Wall of container is observed in portion, moves on the sponge of lower openings
Plant the rice seedlings that seedling age is 15 days, and allow root pass sponge and to contact with bottom reservoir Middle nutrition liquid.
Preferably, the device that this utility model provides is observed container by top and reservoir forms, described sight
Examining container and surrounded cone by sidewall, have upper and lower two openings, volume is 500 milliliters of spaces；Described nutrition
Liquid container is surrounded by bottom and sidewall and has a reservoir opening；Top is observed the under shed of container and is positioned at
In nutrient container, and observe container and combine removably with reservoir, wherein reservoir
In equipped with the nutritional solution of 10 milliliters；
The upper and lower part opening observing container on top is plugged with the circular foam-rubber cushion that thickness is 1cm, wherein,
Plant on the sponge of lower openings and be implanted with the accelerating germination of 5-10 grain (sense worm rice varieties TN1, International Rice Research Institute
Thering is provided, this laboratory is reserved seed for planting) rice, cultivating the rice seedling of 15 days under the conditions of 25 DEG C can use, wherein,
The root of Oryza sativa L. through sponge and contacts with nutritional solution.
In yet another aspect, this utility model provides a kind of method raised and observe planthopper, and the method includes:
Thering is provided a kind of device for raising and observe planthopper, this device includes: this device is observed by top
Container and reservoir composition, described observation container is surrounded cone by sidewall, has upper and lower two openings,
Volume is 500 milliliters of spaces；Described reservoir is surrounded by bottom and sidewall and has a reservoir
Opening；Top is observed the under shed of container and is positioned at nutrient container, and observes container and reservoir with can
The mode of dismounting combines, wherein equipped with the nutritional solution of 10 milliliters in reservoir；Container is observed on top
Upper and lower part opening is plugged with the circular foam-rubber cushion that thickness is 1cm, wherein, is provided with on the sponge of lower openings
Miao Ling is the rice seedlings of 15 days, and wherein, the root of rice seedlings passes the sponge of lower openings and contacts with nutritional solution；
After the female worm of planthopper after 1 day that sprouts wings is connect on worm rice seedling in observing container, then put observing container
Put in reservoir, allow the root system of rice seedling be immersed under the liquid level of reservoir and seal nutritional solution and hold it
The opening of device；
Then, then this device is placed in artificial lighting climate box cultivation, the cultivation that artificial lighting climate box sets
Condition is: temperature: 28 degrees Celsius, and L:D=16:8h illuminance is 5500lx, and relative humidity is 75% 80%.
Preferably, it is also possible on the sponge of lower openings, sow the rice paddy seed of accelerating germination, be then placed into
Putting into the constant incubator nursery of 25 DEG C, after 10-15 days, rice seedling root system can pass from foam-rubber cushion；Seedling is provided
Age was at the rice seedling of about 15 days.
In some preferred modes, the method also includes that container is observed on the top fitted together and nutritional solution holds
Device be placed on natural conditions or set humiture growth cabinet in carry out experimental observation.
In other preferred modes, when needing to investigate when surviving situation of planthopper, take off top and observe container,
The activity situation of planthopper just be can be observed through outer wall；After investigation terminates, then top observation container is placed on
On reservoir.
Preferably, the method can also include allowing top observe container and reservoir is all transparent, or only sees
It is transparent for examining container.
Preferably, most root system is allowed to observe the circular hole of container through top and expose 2-3cm outside, root system
Can be immersed in nutritional solution.
In other preferred modes, when needing accurate metering, top is observed container and takes off and be upside down in glass
In beaker, rapping container, to make the plant hopper on rice seedling shake off in beaker the most countable, again by plant hopper weight after having investigated
Newly connect worm on rice seedling, after experiment terminates, observation container is placed in reservoir.
It is partitioned into the local microenvironment that upper and lower two dry and wet are different, the environment solution that top is more dried by foam-rubber cushion
Rice seedling of having determined is mouldy to rot and the problem of tube wall mist pearl impact, and the nutritional solution of bottom is then the Root Absorption battalion of rice seedling
Support offer condition, it is ensured that rice seedling can be survived the long period, meet laboratory observation demand, reduce replacing host and plant
The number of times of thing.Simultaneously as rice seedling is fixed by the foam-rubber cushion of end opening, it is beneficial to shake off the planthopper on rice seedling
After pour out accurate metering, improve accuracy.This utility model is that planthopper researchers provide a set of research rice
The simple and easy method of the insects such as plant hopper and device, it addition, by the research of the different experimental conditions of this device, permissible
Lay eggs in a large number, it is also possible to be used for producing substantial amounts of worm's ovum for experimentation.
Accompanying drawing explanation
Fig. 1 is the device schematic diagram that planthopper raised by traditional test tube, and wherein 100 represent teat glass, 200 tables
Showing rice seedlings, 300 represent the nutritional solution bottom test tube, and 400 represent the sponge sealing test tube.
Fig. 2 is raising and the device schematic diagram of observation planthopper of this utility model device, and wherein 20 represent sealing
The sponge of upper opening, 10 represent the space accommodating Oryza sativa L., and 30 represent rice seedlings, and 40 represent that sealed lower portion is opened
The sponge of mouth, 50 represent the root of rice seedlings, and 60 represent reservoir, and 70 represent nutrient liquids.
Beneficial effect
(1), this utility model provides a kind of practical, succinct, easily for the scientific research personnel carrying out planthopper research
Research method, the local microenvironment different by being suitably separated out dry and wet, and cultivate the rice seedling of stalwartness for planthopper
Taking food, nutritional solution can extend the time-to-live of rice seedling, reduces the number of times changing host plant, it is to avoid sending out of rice seedling
The mould problem rotted and be difficult to observe because of the impact of mist pearl, it is ensured that experimental error minimizes.(2), described method letter
Single, workable；Avoid and affect experimental result because planthopper escapes when conducting a research.(3), we
Method also has easily observation, and the advantage such as take up room little, and the scientific research being suitable for Study On Rice rhizome portion insect makes
With.
Detailed description of the invention
1 materials and methods
1.1, the preparation of device.
Thering is provided a kind of device (as shown in Figure 2), this device is observed container 10 and reservoir 60 by top
Composition, described observation container is surrounded cone by sidewall, has upper and lower two openings 21,41, and volume is 500
The inner space of milliliter；One reservoir is surrounded by bottom and sidewall and has an opening；Observe under container
Opening 41 is positioned at reservoir not in contact with nutritional solution 70, the observation container on top and reservoir with
Dismountable mode combines, wherein equipped with the nutritional solution of 10 milliliters in reservoir；
The circular sponge that the upper and lower part opening 21,41 thickness 1cm beyond the Great Wall of container is observed on top is got out in advance
Pad (20,40), by 5-10 grain accelerating germination, ((International Rice Research Institute provides sense worm rice varieties TN1, originally
Laboratory is reserved seed for planting) seed rice tweezers dibbling on foam-rubber cushion, (on sponge, absorb the nutritional solution of about 10 milliliters),
Putting into the constant incubator nursery of 25 DEG C, after 15 days, rice seedling can use, and wherein the root of Oryza sativa L. passes sponge
And contact with nutritional solution, absorb nutrition supplying continued propagation.It is of course also possible to directly on the sponge of lower openings
Transplanting seedling age is the rice seedlings 30 of 15 days, and allow root 50 through sponge and with bottom reservoir Middle nutrition
Liquid contacts, and wherein, the formula of nutritional solution is: NH4NO3: 114.3mg/L, NaH2PO4·2H2O:50.4mg/L,
K2SO4: 89.3mg/L, CaCl2: 158.2mg/L, MgSO4·7H2O:405mg/L.
1.2, condition of culture: employing artificial lighting climate box (RXZ-380B type Ningbo south of the River instrument plant) arranges and is
Row steady temperature: 20,24,28 and 32 degrees Celsius.The L:D=16:8h of all process, illuminance is 5500lx,
Relative humidity is 75% 80%.
Examples of implementation 1: examination worm's ovum nest Observation On The Development
By the female worm that newly sprouts wings from the beginning of the 1st day, access in device of the present utility model, according to the modes of 1.2 joints
Cultivate, then the development of ovary rank under anatomic observation condition of different temperatures, each temperature 3 repetition, often
Individual repeat about 10 female worms, the mature egg grain number in the growths rank of record ovary and oviduct, these emergence female
Property insect still this utility model device carrying out continue raise.The development of ovary of planthopper is divided into 5 ranks:
0 grade-1 grade, ovary is not grown, 2 grades, prematuration period, 3-4 level, ovary maturity, spawning period；5 grades, ovum
Nest fails, end of laying eggs.By dissecting classification, can determine whether the development of ovary degree of planthopper.
Meanwhile, at conventional test tube method, (rice seedling and planthopper worm alive are placed in big glass tubing (500 milliliters of volumes
Space), in vitro place the nutritional solution supply rice growth of same volume (10 milliliters), wherein simultaneously
Making the volume of test tube consistent with spatial volume of the present utility model) the female worm raised of condition is from the beginning of the 2nd day, also
Carry out dissecting classification as comparing according to preceding method.
Result: temperature is on the impact of the different days female worm development of ovary after sprouting wings
After emergence from the point of view of the overall development of ovary situation of different times white backed planthopper: along with the rising of temperature, ovary
Rate of development is accelerated, but, only after emergence the 6th day, III-IV grade was respectively 92%, illustrates in temperature
It is laying eggs in a large number of the best in quality of the development of ovary at 28 DEG C, beneficially later stage.The white backward flight raised with this device
The ovarian development of louse to be faster than the test tube method (table 1.1, table 1.2) of routine.On the contrary, traditional dress is used
Putting and raise white backed planthopper, no matter at a temperature of what, there is different ratios in each development of ovary stage, and
And distribution ratio more uniform, be unfavorable for laying eggs of later stage because be intended to as far as possible with which ovary at III-IV grade
Female worm lays eggs, and so can substantial amounts of lay eggs.
Table 1.1 white backed planthopper female worm ovarian development (this device)
The data such as table 1.2. that conventional test tube method experiment obtains
Table 1.2 white backed planthopper female worm ovarian development (test tube method)
(in table, III-IV grade of ovary proportion is the highest, illustrates more to be beneficial to breeding and the growth of white backed planthopper).
Examples of implementation 2: the ovum phase observes.
Similar to examples of implementation 1, female for single head planthopper worm is accessed on rice seedling (this utility model device), not
The female worm of synthermal lower raising copulation, changes fresh rice seedling and lay eggs for female worm every day, each temperature strip of statistic record
Female worm under part lays eggs quantity, until adult is the most dead, adds up the planthopper ovum grain number under each temperature conditions.
Make comparisons with test tube method simultaneously.The condition of culture of device is identical with examples of implementation 1.Result is as follows.
Mature egg grain quantity (this device) of table 2.1 white backed planthopper female worm's ovum pipe
Mature egg grain quantity (test tube method) of table 2.2 white backed planthopper female worm's ovum pipe
White backed planthopper egg laying amount inside this utility model device is can be seen that from the result of table 2.1 and 2.2
Test tube method to be significantly higher than, and use apparatus and method of the present utility model, it is found that this utility model
At different temperatures, the quantity laid eggs also differs device, within latter 6 days, lays eggs if using to sprout wings, then may be used
To significantly improve, than other process, the quantity laid eggs, this may be in maturation with the development of ovary of female worm and be in
The identical reason of progression.This is conducive to the later stage to obtain adult in a large number for biological experiment.On the contrary, traditional
The effect of test tube method is undesirable.
Examples of implementation 3 Life of Adult observes (this utility model device)
The adult newly sprouted wings is linked in this utility model device, the illumination training being placed under condition of different temperatures
Support in case, the life-span of observed and recorded adult.Make comparisons with test tube method simultaneously.
Table 3 temperature Whitebacked Planthopper female adult worm aging effects (my god)
From the results shown in Table 3, in this device, the life-span test tube method to be longer than of white backed planthopper, more sharp
In laboratory observation, at a temperature of 28 DEG C, significantly extend the life-span simultaneously.
Claims (3)
1. the convenient device observed and raise planthopper, it is characterised in that this device is observed container by top and reservoir forms；Described observation container is surrounded cone by sidewall, has upper and lower two openings, and volume is the inner space of 500 milliliters；Described reservoir is surrounded by bottom and sidewall and has an opening；Observing container under shed and be positioned at reservoir not in contact with nutritional solution, the observation container on top combines removably with reservoir, wherein equipped with the nutritional solution of 10 milliliters in reservoir；The circular foam-rubber cushion of the upper and lower part opening thickness 1cm beyond the Great Wall of container is observed on top, transplants the rice seedlings that seedling age is 15 days on the sponge of lower openings, and its root through sponge and contacts with bottom reservoir Middle nutrition liquid.
Device the most according to claim 1, it is characterised in that described container of observing is transparent.
Device the most according to claim 1, it is characterised in that the described material observing container is transparent plastics material.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620193895.XU CN205695091U (en) | 2016-03-14 | 2016-03-14 | A kind of convenient device observed and raise planthopper |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620193895.XU CN205695091U (en) | 2016-03-14 | 2016-03-14 | A kind of convenient device observed and raise planthopper |
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| Publication Number | Publication Date |
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| CN205695091U true CN205695091U (en) | 2016-11-23 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201620193895.XU Expired - Fee Related CN205695091U (en) | 2016-03-14 | 2016-03-14 | A kind of convenient device observed and raise planthopper |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962294A (en) * | 2017-04-05 | 2017-07-21 | 山西省农业科学院植物保护研究所 | A kind of method of aphid indoor feeding |
| CN106962293A (en) * | 2017-04-05 | 2017-07-21 | 山西省农业科学院植物保护研究所 | A kind of single head diamondback moth indoor feeding method |
| CN114831084A (en) * | 2022-06-13 | 2022-08-02 | 湖南省植物保护研究所 | Method for feeding rice planthoppers by utilizing ratoon rice |
-
2016
- 2016-03-14 CN CN201620193895.XU patent/CN205695091U/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106962294A (en) * | 2017-04-05 | 2017-07-21 | 山西省农业科学院植物保护研究所 | A kind of method of aphid indoor feeding |
| CN106962293A (en) * | 2017-04-05 | 2017-07-21 | 山西省农业科学院植物保护研究所 | A kind of single head diamondback moth indoor feeding method |
| CN106962293B (en) * | 2017-04-05 | 2020-07-10 | 山西省农业科学院植物保护研究所 | A kind of indoor breeding method of single-headed diamondback moth |
| CN106962294B (en) * | 2017-04-05 | 2020-07-10 | 山西省农业科学院植物保护研究所 | A method of indoor breeding of aphids |
| CN114831084A (en) * | 2022-06-13 | 2022-08-02 | 湖南省植物保护研究所 | Method for feeding rice planthoppers by utilizing ratoon rice |
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