CN105379683B - A kind of artificial feeding method of wheat moth - Google Patents

Abstract

The present invention provides a method for artificial feeding of wheat moths, which comprises the following three stages: a collecting stage of egg masses in an adult stage, a feeding stage in a larval stage, a pupal stage, and a feeding stage in the adult stage. The present invention utilizes: 1. the adults have the habit of climbing upward and the habit of laying eggs in the crevices, and by providing suitable crevices and the method of pressing the filter paper, the egg mass of the wheat moth is collected, which is convenient to accurately count the daily egg production and the single egg mass of the wheat moth. The number of eggs laid by females; the method of collecting egg mass effectively avoids damage to the egg mass; ② the hatched larvae have the habit of spinning and drooping, and using a brush to hang the silk directly into the finger-shaped tube can effectively avoid the process of infestation. damage to newly hatched larvae; ③ During the feeding process of larvae and pupae, a fully transparent finger-shaped single-pass glass tube and corresponding feed and sealing technology were selected, which can better satisfy the researcher's tracking of the larvae and pupae of wheat moth Observed.

Description

A kind of artificial feeding method of wheat moth

technical field

The invention relates to a method for raising animals, in particular to a method for artificially raising the moth.

Background technique

Wheat moths belong to the order Lepidoptera, Wheat moth family, and the genus Gramine moth. The insect can harm a variety of food crops, such as rice, wheat, corn, sorghum, etc., as many as 19 species, which occur for many generations a year, harms the grain germ, and seriously affects its germination ability. Most of them are bored into empty shells, and the average loss is about 13.1%-24%. They are important stored grain pests. Scholars at home and abroad have done a lot of research on the wheat moth, but because the living place of the beetle is hidden, the research on its biological characteristics is not perfect, especially the growth and development process of the wheat moth is not very clear, which affects the effective control of the wheat moth .

At present, various natural enemy insects, such as Trichogramma, lady beetle, golden wasp, etc., have been reported to use the eggs of wheat moth as live feed. Therefore, exploring a simple method for collecting eggs of wheat moth can lay a foundation for the research on indoor breeding and reproduction of natural enemy insects. Wheat moth is one of the three important world-wide grain storage pests, and it is also a good substitute host for raising natural enemy insects. Indoor breeding of wheat moths has important theoretical and practical significance. The wheat moth is a complete metamorphosis insect, and its life includes four stages: egg, larva, pupa and adult. The adults are small (body length 0.4-0.6 cm), soft and tend to lay eggs in hidden crevices; newly hatched larvae enter the corn kernels directly from the base of the corn caryopsis or damaged parts to feed and damage until the adult emerges, starting from the reserved The feathering hole is drilled out. Both the larval and pupal stages of the moth live in the grain, and its growth and development cannot be observed. At present, the observation and collection method of wheat moth includes: egg mass collection, feeding observation of larvae, feeding and observation of pupal stage, etc., wherein, egg mass collection includes the following steps: 1. Take a container with grain seeds or empty, and access the eclosion on the same day. Adults (≤24h); 2. The adults of the wheat moth lay eggs directly on the inner wall of the container or on the surface of the grain, and pass the inspection every day, and use a brush to brush off the initial laying egg mass attached to the inner wall of the container and the grain; 3. Wait for the adult wheat moth to lay eggs 4. Cut the filter paper into a long strip, fold it multiple times in a "Z" shape, and put it into a container. By replacing the filter paper strip every day, the daily eggs of the moth (eggs within 24 hours) can be collected. ), and count the daily egg laying amount and single female egg production. The above method of collecting egg masses is easy to cause mechanical damage to the eggs, affecting their development and hatching, and is also not conducive to the laying of eggs by the moth, and cannot accurately record the egg production of adults. The flakes are put into a container with grain for feeding. If you need to observe the growth and development of the larvae, you need to cut open the grain with the worms. The feeding observation in the pupal stage includes: cutting the grains and observing the growth and development of the pupa. At present, this kind of rearing method has the following defects: due to the observation of the growth and development process of the larvae and pupa, the grain grains need to be cut open, which destroys the living environment of the larvae and the pupa or damages the worms, affects the growth and development of the larvae, thereby affecting the accuracy of experimental data.

SUMMARY OF THE INVENTION

The object of the present invention is to solve the above-mentioned defects in the prior art, and to provide a method for artificial breeding of wheat moths that can directly, simply and easily collect the egg masses of wheat moth and does not cause mechanical damage to the collected egg masses. During the feeding process of larvae and pupae, a fully transparent finger-shaped single-pass glass tube and corresponding feed and sealing technology are selected to make the growth and development process of larvae and pupae with relatively secretive habits "perspective", and can be better Meet the researchers' follow-up observations on the larvae and pupae of the moth.

A method for artificial rearing of wheat moth, comprising the following steps:

1. Collection of egg masses in adult stage

Select 5 pairs of paired adult worms that have emerged on the same day, insert them into an octagonal bottle with a diameter of 9cm and a height of 15cm, seal with gauze, and place the bottle mouth upward. It is a 12.5cm filter paper, and then cover the top of the filter paper with a large petri dish with a diameter of 12cm, and collect and count the egg masses of the day;

Two, larval stage, pupal stage feeding

(1) Select a finger-shaped single-pass glass tube with a diameter of 1cm and a length of 3cm, put it in an oven at 120°C for sterilization, take it out after 2 hours, and after the glass tube is cooled, put it in a 1.5-2mm artificial glass tube. Feed 0.01-0.05g, use a writing brush to hang the silk-spun larvae into the finger-shaped tube;

(2) Seal the mouth of the finger-shaped tube with Parafilm sealing film, tie fine ventilation holes on the sealing film, and then press a circular tissue paper with a diameter of 2cm on the sealing film to make the tissue paper stick to the sealing film. Put it into an artificial climate box at 27°C, with a relative humidity of 75%, within 24 hours a day, turn on the light and turn on the light: turn off the light and the dark time is 12:12;

(3) The behavioral activities and growth and development process of the larvae and pupa can be observed at any time or at regular intervals through the finger-shaped tube;

(4) when the larvae grow and develop to 3 instars, add 0.1 g of feed, and use cotton plugs to replace Parafilm and tissue paper to plug the finger-shaped nozzles;

3. Feeding during the adult period

When the wheat moths raised in the finger-shaped tube have emerged, the adults are taken out immediately, paired in an octagonal bottle according to the ratio of male to female ratio of 1:1, placed in a relative humidity of 75%, within 24 hours a day, turn on the light Time: In an artificial climate box with a dark time of 12:12, the animals were fed with 10% honey water to supplement nutrition.

Further, according to the above-mentioned artificial breeding method of wheat moth, the specification of the gauze is 40 meshes.

Further, in the above-mentioned artificial feeding method of wheat moth, in the step of collecting egg masses in the adult stage, comprising: selecting a small petri dish with a diameter of 3.5cm, laying absorbent cotton in the dish, pouring honey water with a concentration of 10%, Make the absorbent cotton completely infiltrated and in a saturated state, then put the petri dish into an octagonal bottle to supplement nutrition for the adults, and replace the small petri dish with honey water every day.

Beneficial effects:

1. It can provide a direct, simple and easy method for collecting egg masses, and this method does not cause mechanical damage to the collected egg masses;

2. It can accurately record the daily egg production and the single female egg production;

3. Can intuitively and directly observe the growth and development process of the egg stage, larval stage and pupa stage of the moth;

4. It can be extended and applied to the research on the boring stored grain pests. Such as: corn elephants, etc.

Detailed ways

In order to make the purpose, technical solutions and advantages of the present invention clearer, the technical solutions in the present invention are described clearly and completely below. Obviously, the described embodiments are a part of the embodiments of the present invention, not all of the embodiments. Based on the embodiments of the present invention, all other embodiments obtained by those of ordinary skill in the art without creative efforts shall fall within the protection scope of the present invention.

The present invention improves the method of daily egg mass collection. Using the habit of adults to climb up and tend to lay eggs in crevices, the egg mass of the moth was collected by pressing the filter paper, which is also convenient for accurate statistics of the daily egg production and single female egg production of the moth; the egg mass was collected with filter paper. The method avoids damage to the egg mass, and the hatched larvae use the habit of spinning and drooping to facilitate access to the finger-shaped tube.

The present invention provides feed traits, container and sealing technology for single-headed wheat moths. The sealing technology can avoid the lack of food due to the tendency of the larvae to the gap, resulting in accidental death; the characteristics of the feed and the container used can enable researchers to observe the growth and development of the entire larval and pupal stages, and also provide research for other borer pests. in accordance with.

One, a kind of artificial feeding method of wheat moth, this method comprises the following steps:

1. Collection of egg masses in the adult stage: Select 5 pairs of healthy adults that have emerged on the same day and put them into an octagonal bottle, use a 40-mesh gauze to seal, and place the bottle mouth upward; outside the octagonal bottle, put a cover on top of the sealed gauze. filter paper, and then cover the top of the filter paper with a petri dish with a diameter of 12 cm. The filter paper slips and shakes; the place where the filter paper and the gauze meet is the crevice that is artificially provided for the egg-laying of the female moth. By changing the filter paper regularly every day, the number of egg masses and egg particles on the filter paper can be collected and counted;

2, larval stage, pupal stage feeding

(1) Select a finger-shaped single-pass glass tube with a diameter of 1cm and a length of 3cm, put it in an oven at 120°C for sterilization, take it out after 2 hours, and after the glass tube is cooled, put it in a 1.5-2mm artificial glass tube. Feed 0.01-0.05g, use a writing brush to hang the silk-spun larvae into the finger-shaped tube;

(2) Seal the mouth of the finger-shaped tube with Parafilm sealing film, then tie air micropores on the film, and then press a circular tissue paper with a diameter of 2 cm on the sealing film to make the tissue paper stick to the sealing film It can not only effectively meet the ventilation of the young larvae, but also effectively prevent the larvae from escaping. Put it into an artificial climate box at 27°C, with a relative humidity of 75%, within 24 hours a day, turn on the lights and turn on the lights: turn off the lights and the dark time is 12:12;

(3) The behavioral activities and growth and development process of the larvae and pupa can be observed at any time or at regular intervals through the finger-shaped tube;

(4) when the larvae grow and develop to 3 instars, add 0.1 g of feed, and use cotton plugs to replace Parafilm and tissue paper to plug the finger-shaped nozzles;

(5) During the feeding process of the larval and pupal stages, if necessary for experiments, single-tube single-head feeding (each glass tube is connected to only one healthy newly hatched larva) can be used for researchers to collect relevant "data".

3. Feeding during the adult stage

When the wheat moths raised in the finger-shaped tube have emerged, the adults are taken out immediately, paired in an octagonal bottle according to the ratio of male to female ratio of 1:1, placed in a relative humidity of 75%, within 24 hours a day, turn on the light Time: In an artificial climate box with a dark time of 12:12, the animals were fed with 10% honey water to supplement nutrition.

During the egg mass collection step in the adult stage,

(1) Select a petri dish with a diameter of 3.5cm, spread absorbent cotton in the dish, pour honey water with a concentration of 10%, make the absorbent cotton fully infiltrated, and be in a saturated state, then put the petri dish into an octagonal bottle (octagonal bottle specification: d=9cm, h=15cm), supplement nutrition to adults, and replace the petri dish with honey water every day;

(2) Select healthy 5 pairs of adults (≤24h) that emerged on the same day and put them into an octagonal bottle, and seal them with a gauze of about 40 meshes. Because the moth has the habit of putting the ovipositor into the gauze to lay eggs, a filter paper with a diameter of 12.5cm is covered with a petri dish on the gauze at the mouth of the bottle, and the filter paper can be replaced every day. Collect and count the day. egg mass.

Two, larval stage, pupal stage feeding

(1) Select a fully transparent finger-shaped tube with a diameter of 1 cm and a length of 3 cm, place it in an oven at 120 ° C for sterilization, take it out after 2 hours, and after the glass tube is cooled, put in artificial feed with a size of about 1.5-2 mm (approximately 0.02g), according to the habit of the larvae of the moth larvae spun silk, put the silk larvae into the finger-shaped tube with a writing brush to avoid direct contact with the writing brush and cause damage to the larvae;

(2) Use a Parafilm sealing film with a size of 1.5cm*1.5cm, tie the air holes on the sealing film with a microneedle, press a circular tissue paper with a diameter of 2 cm on the sealing film, and apply a little force to make the tissue stick. On the parafilm, put it into an artificial climate box at 27°C, RH=75%, L:D=12:12;

(3) Through the finger-shaped tube, we can observe that the larvae spin silk and stick the feed to the inner wall of the finger-shaped tube, and the larvae are hidden between the tube wall and the food, and the growth and development process of the larvae and pupa of the wheat moth can be easily observed;

(4) After the 3rd instar larvae, the upper jaws of the mouthparts become very developed, their food intake increases significantly, and it is easy to bite the sealing film and tissue paper to escape. Therefore, when the larvae enter the 3rd instar, add 0.1g of feed , and seal with a tampon.

Example 1:

Using this method:

①In August 2014, select 5 pairs of paired adults (≤24h) that have emerged on the same day, put them in an octagonal bottle containing 10% honey water absorbent cotton, seal them with about 40 mesh gauze, and cover the gauze outside the bottle mouth with a A piece of filter paper with a diameter of 12.5 cm was placed on top of the filter paper and a petri dish (the diameter of the petri dish was 12 cm) was reversed to collect and count the egg masses of the day.

②Put the filter paper with egg mass into the artificial climate box (rearing conditions: 27°C, relative humidity of 75%, within 24 hours a day, turn on the light and illuminate the time: turn off the light and dark time is 12:12), cultivate to the seventh day, about 90% of the larvae hatch. The silks spun by the newly hatched larvae were attached with a writing brush and put into a glass finger tube containing feed (one larva per tube, 40 tubes in total), and sealed with Parafilm and tissue paper. Continue feeding in an artificial climate box.

③ Observe and record the behavior, growth and development of larvae regularly every day.

④ Around the 32nd day, when the larvae developed to the third instar, 0.1 g of feed was added to the finger-shaped tube, and cotton plugs were used to replace the parafilm and tissue paper.

⑤It was observed and recorded that the larvae pupated on the 47th day, and the pupae emerged into adults on the 52nd day.

Example 2:

In September 2014, the content of Example 1 was repeated.

Example 3:

In October 2014, the content of Example 1 was repeated again.

The results are shown in Table 1

Table 1

in conclusion:

(1) Through this method, a large number of required egg masses can be collected to meet the needs of various relevant experiments and experiments; the daily egg production and single female egg production of the moth can be accurately counted, and the error rate between the results and the actual results can be compared. Compared with previous methods, it is much lower and has higher accuracy.

(2) Through this method, the entire generation of the wheat moth can be completely completed, (the generation of the wheat moth is the life of the wheat moth, and its life has experienced four insect states: egg, larva, pupa, and adult).

(3) Through the rearing method of perspective, in the feeding process of the wheat moth, the behavior and growth and development process of the eggs, larvae, pupa, and adults of the wheat moth can be observed at any time or regularly, which is convenient for observing and recording the morphology, Behavioural and ecological characteristics, which have not been achieved by predecessors.

(4) This method provides a reliable reference for feeding and related research of borer pests. Such as: corn elephants, mung bean elephants, etc.

Remark:

Parafilm

Brand: American Parafilm

Product name: Parafilm sealing film

Item number: PM-996

Packaging specification: 100mm*38M

Price: 139 yuan/roll

The present invention has the following advantages:

1. The method of collecting egg mass has been improved, which is simpler and easier to implement than the previous method, and the statistics of daily egg production and single female egg production are more accurate;

2. Reduced mechanical damage to egg mass and newly hatched larvae;

3. A more effective "see-through" method for rearing larvae and pupae, as well as materials matched with the method, and containers used for rearing are proposed. The method is easy to operate, and by adopting the method, not only can the larvae of each instar stage of the wheat moth be observed, but also the development process of pupation and pupa, and the whole process of eclosion can be observed. It breaks the phenomenon that insects can only be seen by dissecting grains in the past, and effectively fills the "perspective" observation gap of "pupation", "emergence" and other processes.

The invention utilizes the habit of adults to climb upward and tend to lay eggs in crevices, and collects the egg mass of the wheat moth by providing a suitable crevice and a method of pressing filter paper, so as to accurately count the daily egg production and single female production of the wheat moth. The egg mass; the method of collecting egg mass effectively avoids damage to the egg mass, and the hatched larvae have the habit of spinning and drooping. Using a brush to hang the silk directly into the finger-shaped tube can effectively avoid the process of infesting the first hatching. Injury to larvae; the present invention provides feed properties, container and sealing technology for penetrating observation feeding and single-head penetrating observation feeding wheat moths. The container can allow researchers to observe the growth and development of feeding insects throughout the larval and pupal stages, and also provide a reference for the study of other borer pests.

Finally, it should be noted that the above embodiments are only used to illustrate the technical solutions of the present invention, but not to limit them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that it can still be The technical solutions described in the foregoing embodiments are modified, or some technical features thereof are equivalently replaced; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the spirit and scope of the technical solutions of the embodiments of the present invention.

Claims (3)

1. a kind of gelechiid rearing method, which comprises the following steps:

One, adult stage pieces of an egg are collected

The pairing adult of 5 pairs of same day emergence is chosen, access diameter is to be sealed with gauze, bottle in the octagonal bottle of 9cm, a height of 15cm Mouth is placed upwards, outside octagonal bottle, covers the filter paper that a diameter is 12.5cm in the upper surface of gauze of sealing, then on filter paper The anti-big culture dish for covering diameter and being 12 centimetres in side, collects and counts same day pieces of an egg；

Two, larval phase, pupa time raising

(1) choosing diameter is 1cm, and the finger-type single-pass glass tube of a length of 3cm all-transparent is placed in 120 DEG C of baking oven and sterilizes, after 2h It takes out, after glass tube is cooling, is put into the man-made feeds 0.01-0.05g that size is 1.5-2mm, with writing brush by the larva of spinning Hanging wire accesses in dactylethrae；

(2) nozzle of dactylethrae is sealed with Parafilm sealed membrane, then to pricking subtle venthole on sealed membrane, then will be straight Diameter is the round cotton paper gland of 2cm on sealed membrane, is sticked to cotton paper on sealed membrane, is put into 27 DEG C, relative humidity 75%, one Within it 24 hours, light application time of turning on light: raised in the growth cabinet that interlunation is 12:12 of turning off the light；

(3) behavioral activity and growth and development process of gelechiid larva and pupa can at any time or be periodically observed through dactylethrae；

(4) when larva growth and development to 3 ages and then addition 0.1g feed, and select tampon replacement Parafilm sealed membrane and Cotton paper clogs finger-type nozzle；

Three, the adult stage raises

After the gelechiid raised in dactylethrae sprouts wings, adult is taken out at once, is put into illiciumverum according to the proportion pairing that sex ration is 1:1 In bottle, being placed in relative humidity is 75%, within 24 hours one day, light application time of turning on light: interlunation of turning off the light is the artificial of 12:12 In climate box, is supplemented the nutrients and raised with 10% hydromel.

2. gelechiid rearing method according to claim 1, which is characterized in that the specification of the gauze is 40 mesh.

3. gelechiid rearing method according to claim 1, which is characterized in that the adult stage pieces of an egg collect step In, comprising: selecting diameter is the small culture dish of 3.5cm, spreads absorbent cotton in ware, pours into the hydromel that concentration is 10%, makes degreasing Cotton complete wetting is in saturation state, then culture dish is put into octagonal bottle to Adult supplement nutrient, and replacement is with hydromel daily Small culture dish.