CN205443335U - A cell culture device based on a microfluidic chip - Google Patents

Abstract

The utility model discloses a cell culture device based on a microfluidic chip, comprising a main part, a control panel, a timer, a display, a cover, a microfluidic chip, a temperature control plate, a cell culture pool, a hole chamber, and a microfluidic channel. The main part is provided with a flip cover connected by a shaft, and a cell culture area is arranged under the flip cover, and a microfluidic chip is arranged in the cell culture area, and a cell culture pool and a microfluidic channel are arranged on the microfluidic chip. , the microfluidic channel communicates with each cell culture pool respectively, and a plurality of chambers are arranged in the cell culture pool. Compared with the prior art, the beneficial effect of the utility model is that the new type of cell The culture device, which can provide the best environment for cell culture, is equipped with multiple cell culture pools, and can perform various cell culture experiments. At the same time, due to the design of the microfluidic chip, it can detect and monitor the status of cell culture at any time and quickly.

Description

A cell culture device based on a microfluidic chip

technical field

The utility model relates to a cell culture device, in particular to a cell culture device based on a microfluidic chip.

Background technique

Cell culture Cell culture technology is also called cell cloning technology, and the formal term in biology is cell culture technology. No matter for the entire bioengineering technology or one of the biological cloning technologies, cell culture is an essential process, and cell culture itself is a large-scale cloning of cells. Cell culture technology can transform a single cell into simple single cell or rarely differentiated multi-cell through mass culture, which is an essential part of cloning technology, and cell culture itself is cell cloning. A large number of cells or their metabolites are obtained by cell culture. Because biological products are derived from cells, it can be said that cell culture technology is the core and basic technology in biotechnology.

With the continuous development of biotechnology, there are many devices for cell culture. However, the existing cell culture devices may carry out purposeful cell culture. They cannot control the culture process and the changes in the substances of the cells during the decomposition process. It is not convenient to understand the cell culture process more accurately and control the results of cell culture.

Contents of the invention

The purpose of this utility model is to provide a cell culture device based on a microfluidic chip to solve the problems raised in the above-mentioned background technology.

The purpose of this utility model is achieved through the following technical solutions: a cell culture device based on a microfluidic chip, including a main part, a control panel, a timer, a display, a flip cover, a microfluidic chip, a temperature control plate, and a cell culture device. The main part is provided with a flip cover connected by a shaft, and a cell culture area is arranged under the flip cover, and a microfluidic chip is arranged in the cell culture area. On the microfluidic chip A cell culture pool and a microfluidic channel are provided, and the microfluidic channel communicates with each cell culture pool respectively, and a plurality of chambers are arranged in the cell culture pool, and a temperature control plate is provided at the bottom of the microfluidic chip, and the The microfluidic chip is connected to the built-in processor of the main part through the counter, and the processor is respectively connected to the temperature control board, temperature sensor, humidity simulator, humidity sensor, battery, control panel and display, and the temperature sensor , a humidity sensor, a humidity simulator and a temperature control board are all arranged in the above-mentioned cell culture area in the main part, the control panel and the display are arranged on the front of the main part, and the control panel is connected with the timer.

Further, the microfluidic chip is provided with an APD battery.

Further, the flip cover is made of transparent plastic or transparent glass.

Further, the microfluidic chip is made of PDMS polymer, and the depth of the cell culture pool provided on the microfluidic chip is 1mm-5mm.

Compared with the prior art, the beneficial effect of the utility model is that the new cell culture device based on a microfluidic chip can provide the best environment for cell culture, and is equipped with multiple cell culture pools, which can carry out various For cell culture experiments, at the same time, due to the design of the microfluidic chip, the state of cell culture can be detected and monitored at any time and quickly.

Description of drawings

Fig. 1 is the internal principle system diagram of the utility model;

Fig. 2 is the rendering of the utility model microfluidic chip;

Fig. 3 is the overall effect diagram of the utility model;

In the figure: 1. Main part, 2. Control panel, 3. Timer, 4. Display, 5. Flip cover, 6. Microfluidic chip, 7. Temperature control board, 8. Cell culture pool, 9. Well chamber, 10. Microchannel.

detailed description

The technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only part of the embodiments of the present invention, not all of them. For example, based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the scope of protection of the present invention.

As shown in Figures 1 to 3, the utility model discloses a cell culture device based on a microfluidic chip, including a main part 1, a control panel 2, a timer 3, a display 4, a flip cover 5, a microfluidic chip 6, a temperature Control plate 7, cell culture tank 8, hole chamber 9, microfluidic channel 10, the main part 1 is provided with a flip 5 connected by a shaft, a cell culture area is arranged under the flip 5, and a cell culture area is arranged in the cell culture area A microfluidic chip 6, the microfluidic chip 6 is provided with a cell culture pool 8 and a microfluidic channel 10, the microfluidic channel 10 communicates with each cell culture pool 8 respectively, and the cell culture pool 8 is provided with A plurality of chambers 9, the bottom of the microfluidic chip 6 is provided with a temperature control plate 7, the microfluidic chip 6 is connected to the built-in processor of the main part 1 through a counter, and the processors are respectively connected to the temperature control plate 7 , temperature sensor, humidity simulator, humidity sensor, battery, control panel 2 and display 4 are connected, and the temperature sensor, humidity sensor, humidity simulator and temperature control board 7 are all located on the upper part of the main part 1 In the cell culture area, the control panel 2 and the display 4 are arranged on the front of the main part 1, the control panel 2 is connected to the timer 3, and the setting of the timer 3 facilitates the control of the cell division cycle and the setting of the culture time. Record, the built-in humidity simulator and temperature control panel 7 can control the temperature and humidity in the cell culture area at any time to ensure that the cells can be normally cultivated, and the display 4 can display the humidity value and temperature value in the cell culture area, which is convenient for control; The microfluidic chip 6 is provided with an APD battery for easy detection; the flip cover 5 is made of transparent plastic or transparent glass, which is convenient for observing the condition of cells in the cell culture pool 8; the microfluidic chip 6 uses PDMS Made of polymer, the depth of the cell culture pool 8 provided on the microfluidic chip 6 is 1mm-5mm, which provides a better environment for cell culture.

It is obvious to those skilled in the art that the present invention is not limited to the details of the above exemplary embodiments, and that the present invention can be implemented in other specific forms without departing from the spirit or essential features of the present invention. Therefore, no matter from all points of view, the embodiments should be regarded as exemplary and non-restrictive, and the scope of the present invention is defined by the appended claims rather than the above description, so it is intended to fall within the scope of the claims All changes within the meaning and range of equivalents of the required elements are included in the present invention. Any reference sign in a claim should not be construed as limiting the claim concerned.

In addition, it should be understood that although this specification is described according to implementation modes, not each implementation mode only contains an independent technical solution, and this description in the specification is only for clarity, and those skilled in the art should take the specification as a whole , the technical solutions in the various embodiments can also be properly combined to form other implementations that can be understood by those skilled in the art.

Claims (4)

1. A cell culture device based on a microfluidic chip, including a main part (1), a control panel (2), a timer (3), a display (4), a flip cover (5), a microfluidic chip (6), Temperature control plate (7), cell culture pool (8), hole chamber (9), microfluidic channel (10), characterized in that: the main part (1) is provided with a flip cover (5) connected by a shaft, and the A cell culture area is provided under the flip cover (5), and a microfluidic chip (6) is provided in the cell culture area, and a cell culture pool (8) and a microfluidic channel ( 10), the microfluidic channel (10) communicates with each cell culture pool (8), the cell culture pool (8) is provided with a plurality of chambers (9), the microfluidic chip (6) There is a temperature control board (7) at the bottom, and the microfluidic chip (6) is connected to the built-in processor of the main part (1) through a counter, and the processor is respectively connected to the temperature control board (7), temperature sensor, The humidity simulator, humidity sensor, battery, control panel (2) and display (4) are connected, and the temperature sensor, humidity sensor, humidity simulator and temperature control board (7) are all arranged in the main part (1) In the aforementioned cell culture area, the control panel (2) and display (4) are arranged on the front of the main part (1), and the control panel (2) is connected with the timer (3). 2. A microfluidic chip-based cell culture device according to claim 1, characterized in that: said microfluidic chip (6) is equipped with an APD battery. 3. A microfluidic chip-based cell culture device according to claim 1, characterized in that: the flip cover (5) is made of transparent plastic or transparent glass. 4. A microfluidic chip-based cell culture device according to claim 1, characterized in that: the microfluidic chip (6) is made of PDMS polymer, and the microfluidic chip (6) is equipped with The depth of the cell culture pool (8) is 1mm-5mm.