CN204644371U - A kind of integrated form many cells noncontact co-culture device - Google Patents

Abstract

The utility model discloses an integrated multi-cell non-contact co-cultivation device, which comprises a culture bottle body containing two or more bottle mouths, and a permeable membrane is arranged between each two bottle mouths to divide the bottle body into independent the cultivation area. The utility model uses a permeable membrane material to divide the bottle body into several cell culture areas, and only liquid and gas between different cell culture areas can communicate through the permeable membrane material, effectively increasing the area of cell culture and the concentration of substances such as cytokines. The exchange area can realize the mixed culture of various cell lines, effectively increase the concentration of secreted proteins in the culture medium, more realistically simulate the multi-cell interaction and amplification process in vitro, and facilitate the overall system research on the cell interaction of a complete biological system .

Description

An integrated multi-cell non-contact co-culture device

technical field

The utility model relates to a cell culture device, in particular to an integrated multi-cell non-contact co-culture device.

Background technique

Cell culture is to make cells grow and multiply under in vitro conditions. During the culture process, the morphology and structure of living cells can be observed, monitored and data recorded. The in vitro culture of cells can be cultured alone or co-cultured with multiple cells.

Commonly used cell culture devices are mainly single-cell line gas-liquid phase plane culture, whose singleness and immobilization limit the multi-directional development of cell culture, and cannot satisfy the in-depth study of complex systems such as cell interactions. Although there are commercialized co-culture devices at present, there are problems such as contamination and mixing between different cells. The authorized announcement number is CN202181306, and the announcement date is April 4, 2012. The Chinese utility model patent entitled "Multiple Cell Co-Culture Scaffold and Multiple Cell Co-Culture Device" discloses a multi-cell co-culture device. A culture support is provided, and the support includes a support surface and a support frame connected with the support surface, and a fixing hole is opened on the support surface. A support frame is connected to the edge of the support surface. The culture support is put into the culture dish, the fixed holes of the culture support are used to hang the culture chambers, and each culture chamber is used for culturing one kind of cells. Then the culture dish, the co-cultivation support and the suspended culture chamber become a culture system for co-cultivation of various cells. Various cells are grown in different culture chambers or petri dishes. The bottom of the culture chamber is provided with apertures of different sizes, which allow liquid to pass through freely, but cells cannot pass through. However, in this co-culture device, the contact area between the cells and the co-culture is small, and there is a large amount of redundant space, so a large amount of culture fluid needs to be consumed to make the liquid level exceed the suspension culture chamber to maintain the growth of the cells. The increase in the volume of culture medium reduces the concentration of secreted proteins in the culture medium, thereby reducing the influence of secreted proteins in cell interactions, and also poses challenges to sample preparation and analysis for secretome proteomics.

Utility model content

The purpose of the utility model is to make up for the deficiencies of the prior art, and to provide a multi-cell co-cultivation device with practical functions and strong operability, which can meet the research requirements of secretome proteomics.

For solving the problems of the technologies described above, the technical solution of the utility model is:

An integrated multi-cell non-contact co-cultivation device, including a culture bottle body, the bottle body is divided into two or more independent culture chambers, and a permeable membrane is arranged between two adjacent chambers, Each independent culture chamber cover is provided with a chamber port on the wall of the culture bottle.

Further, the permeable membrane is zigzag folded.

Further, plastic brackets are provided at the corresponding positions of the front and rear walls, and the permeable membrane material is fixed on them.

Further, it also includes two or more chamber covers, the size of which is suitable for the chamber opening, and covers each chamber opening respectively.

Further, the chamber port and the chamber cover are respectively provided with threads, and can be connected to each other through threads.

Further, the culture bottle body is made of light-permeable cell culture materials.

Further, the transparent membrane material is a light-transparent material, and the morphology and growth of the cells on the top two transparent membranes can be observed by adjusting the focal length of the microscope.

The integrated multi-cell non-contact co-cultivation device of the utility model uses a permeable membrane material to divide the bottle into left and right cell culture areas, and only liquid and gas between the two cell culture areas communicate through the permeable membrane material. Aiming at the systematization and precision of the in vitro cell culture system and the research requirements of secretome proteomics, the utility model establishes a new and practical co-culture cell culture device, which effectively increases the area of cell culture and the exchange area of substances such as cytokines, It can realize the mixed culture of various cell lines, effectively increase the concentration of secreted proteins in the culture medium, more realistically simulate the multi-cell interaction and amplification process in vitro, and facilitate the overall system research on the cell interaction of a complete biological system.

For better understanding and implementation, the utility model will be described in detail below in conjunction with the accompanying drawings.

Description of drawings

Fig. 1 is the structural representation of the utility model.

Figure 2 is an exploded schematic view of the base of the culture bottle.

Figure 3 is a schematic diagram of a co-culture device for two different types of adherent cells.

Figure 4 is a schematic diagram of a co-culture setup for two different types of cells grown in suspension.

Detailed ways

【Example 1】

Please refer to Fig. 1 and Fig. 2, a kind of integrated multi-cell non-contact co-cultivation device, comprises culture bottle body 1. and two culture bottle caps 2, and described bottle body is rectangular, is connected by bottom wall, top wall and and The distance between the bottom wall and the edge of the top wall is surrounded by the side wall; it also includes a permeable membrane material 3, and the corresponding positions of the front and rear walls in the side wall are provided with plastic brackets, and the permeable membrane material 3 is fixed on the top and connects the front and rear walls, and the bottle body is divided into left and right cell culture chambers 4 by multi-layer folding. The permeable membrane material is upwardly connected to the top wall, and two threaded bottle mouths 5 are arranged side by side on the left and right sides of the top wall, respectively opening on both sides of the permeable membrane material and extending upward, and the size of the culture bottle cap is the same as that of the bottle. Mouth fit. The culture bottle body is made of light-permeable cell culture material, and the permeable membrane material is light-permeable material.

There is a certain distance between the edge of the permeable membrane and the side wall of the culture bottle to form a culture solution perfusion zone; between the two transverse permeable membranes is a cell growth zone; each layer of cell growth zone is alternately connected with the culture solution perfusion zone on the left and right sides .

As shown in Figure 3, from the two openings on the top surface of the base of the culture flask, two different types of adherent cells were added: the cell suspensions of Cell 1 and Cell 2, and the permeability of the two cells in their respective independent spaces. On the membrane material (cells in the bottom layer of the culture flask are on the light-permeable cell culture material) adherent growth. The secreted proteins produced by cell 1 and cell 2 communicate with each other through the permeable membrane material in the permeable frame, and act on cell 1 and cell 2 simultaneously. This device enables cells 1 and 2 to continuously interact with each other through their secreted proteins during in vitro culture, to influence and regulate each other's growth, metabolism and other biological functions. When conducting secretome research and analysis, the culture solution is poured out from the opening on the top surface of the base of the culture bottle, and the samples required for analysis can be obtained after centrifuging to remove the cell debris in the culture solution.

[Example 2]

In Example 2, as shown in FIG. 4 , the culture device has the same structure as that of Example 1, except that the cultured cells are two different types of cells grown in suspension. From the two openings on the top surface of the culture flask base, add two different types of suspended cells: cell suspensions of cell 1 and cell 2, and the two types of cells grow in suspension in cell culture areas in their own independent spaces. The secreted proteins produced by cell 1 and cell 2 communicate with each other through the permeable membrane material in the permeable frame, and act on cell 1 and cell 2 simultaneously. This device enables cells 1 and 2 to continuously interact with each other through their secreted proteins during in vitro culture, to influence and regulate each other's growth, metabolism and other biological functions. When conducting secretome research and analysis, the culture medium is poured out from the opening on the top surface of the base of the culture bottle, and the samples required for analysis can be obtained after centrifuging to remove the cell suspension in the culture medium.

The utility model is not limited to the above-mentioned embodiments, if the various changes or modifications of the utility model do not depart from the scope of implementation of the utility model. All equal changes and improvements made according to the scope of application of the utility model shall belong to the scope covered by the patent of the utility model.

Claims (7)

1. an integrated form many cells noncontact co-culture device, it is characterized in that: comprise a cultivation bottle, described bottle is divided into two or more independently to cultivate chamber, have permeable membrane between two adjacent chambers are arranged, the culturing bottle wall of described each independently culture chamber chamber cap is provided with a chamber port.

2. integrated form many cells noncontact co-culture device according to claim 1, is characterized in that: described permeable membrane is in zigzag rugosity.

3. integrated form many cells noncontact co-culture device according to claim 1 and 2, is characterized in that: described forward and backward two wall correspondence positions are provided with plastic rubber bracket, described permeable membrane material be fixed on described on.

4. integrated form many cells noncontact co-culture device according to claim 1, it is characterized in that: also comprise two or more Pit covers, this training Pit cover size and described chamber port adapt, and are placed on each chamber port respectively.

5. the integrated form many cells noncontact co-culture device according to claim 1 or 4, is characterized in that: described chamber port and Pit cover arrange screw thread respectively, and are interconnected by screw thread.

6. integrated form many cells noncontact co-culture device according to claim 1, is characterized in that: described cultivation bottle adopts the manufacture of translucidus cell culture material.

7. integrated form many cells noncontact co-culture device according to claim 1, is characterized in that: described permeable membrane material is translucidus material.