CN205170866U - No clear tape embryo culture ware - Google Patents

Abstract

The utility model provides an embryo culture dish without zona pellucida, which includes a culture dish cover and a culture dish body, the bottom diameter of the culture dish body is 90 mm, the wall thickness of the culture dish body is 3 mm, and several culture chambers are arranged at the bottom of the culture dish body , the culture chambers are countersunk holes, and the distance between adjacent culture chambers is 0.4cm, which are evenly distributed on the bottom plate of the culture dish. The utility model has the advantages of simple structure, standardized specifications, and large-scale cultivation can be realized.

Description

A kind of embryo culture dish without zona pellucida

technical field

The utility model relates to a zona-free embryo culture device, in particular to an in vitro culture dish for zona-free embryos.

Background technique

The traditional nuclear transfer is carried out on the basis of the integrity of the zona pellucida, because the traditional view is that the zona pellucida is necessary for nuclear reprogramming and the subsequent development of the embryo. When using traditional methods to conduct nuclear transfer research, expensive instruments and equipment are required, and operators are required to master skilled techniques, which limits nuclear transfer research, especially the development of applications. However, recent studies have shown that the zona pellucida is not required for embryonic development. Some scholars have found that fertilized eggs without zona pellucida can develop into blastocysts. However, research on zona-free nuclear transfer still faces a major problem: zona-free embryos tend to aggregate during culture and are not suitable for traditional concentrated culture. Therefore, the research on single embryo culture system is very necessary.

The single embryo culture system is an embryo culture method developed with the development of zona-free nuclear transfer technology, and only a single embryo is cultured in it. The existing traditional single embryo culture method is complex and inefficient, and at the same time cannot allow multiple cultured embryos to be connected during the culture process, and cannot take advantage of the traditional centralized culture of embryos (the factors secreted by concentrated cultured embryos regulate each other to promote embryo development). development).

Contents of the invention

The purpose of this utility model is to provide a non-zona pellucida embryo culture dish for centralized separation and individual culture of embryos, to overcome the problem of easy adhesion of non-zona pellucida embryos in centralized culture, and also to facilitate the cleavage of single embryos Adhesion between balls, thereby improving the efficiency of in vitro culture of zona pellucida embryos.

In order to achieve the above object, the technical scheme provided by the utility model is: a culture dish without zona pellucida, which includes a culture dish cover and a culture dish body, the diameter of the bottom of the culture dish body is 90mm, and the wall thickness of the culture dish body is 3mm , There are several culture cavities at the bottom of the culture dish, the culture chambers are countersunk holes, the distance between adjacent culture chambers is 0.4cm, and they are evenly distributed on the bottom plate of the culture dish.

The culture chamber has a depth of 1000um and a diameter of 400um.

The bottom of the culture dish is provided with a square culture area, and the culture chamber is located in the culture area.

The beneficial effects of the utility model:

First, the structure is simple, and it is convenient to carry out the operation of culturing embryos without zona pellucida.

Second, the device saves materials and culture fluid, and is easy to be accepted by researchers.

Third, standardization of specifications can achieve scale and improve its economic benefits.

Fourth, because it is a disposable consumable, it has great market application value.

Description of drawings

Figure 1 is a schematic diagram of the overall structure of the utility model.

detailed description

Below in conjunction with all accompanying drawings, the utility model is further described, and the preferred implementation of the utility model is: referring to accompanying drawing 1, the culture dish without zona pellucida described in the present embodiment includes a culture dish cover 2 and a culture dish body 1, The diameter of the bottom of the culture dish body 1 is 90mm, and the wall thickness of the culture dish body 1 is 3mm. The bottom of the culture dish body 1 is provided with several culture chambers 3 that can accommodate 125.6ul of culture fluid. The culture chamber 3 is a sink hole, and the depth of the culture chamber 3 is The diameter is 1000um, the diameter is 400um, the distance between adjacent culture chambers 3 is 0.4cm, and they are evenly distributed on the bottom plate of the culture dish body 1 . A square culture area 4 can also be provided at the bottom of the culture dish body 1 as required, and the culture cavity 3 is located in the culture area. The culture dish can effectively enable the zona-free embryos to be cultured separately under concentrated culture solution, can effectively prevent the mutual adhesion of the zona-free embryos, and makes the culture of the zona-free embryos simpler and more efficient. Compared with traditional nuclear transplantation, zone-free nuclear transplantation not only does not require expensive instruments and equipment, but also reduces the work intensity and technical difficulty of operation. At the same time, zona-free nuclear transfer has unique advantages, such as the study of embryo chimerism. This has laid a solid foundation for the research and application of zona-free nuclear transfer technology in the future. At the same time, since transgenic animals or xenogeneic organ transplantation require zona-free nuclear transfer technology, the application of zona-free embryo culture dishes has great potential. Market potential. In practical application, add flat embryo culture medium in the petri dish, then cover with paraffin oil, then you can use the embryo transfer tube to suck up the current embryo without zona pellucida and put one embryo in each well. In this way, a centralized culture system for single culture of embryos without zona pellucida is formed, thereby improving the efficiency of embryo development.

The above-described embodiments are only preferred embodiments of the present utility model, and are not intended to limit the scope of implementation of the present utility model, so all changes made according to the shape and principle of the present utility model should be covered by the scope of the present utility model. within the scope of protection.

Claims (3)

1. a culture of zona-free embryos ware, it is characterized in that: it includes culture dish lid (2) and culture dish body (1), culture dish body (1) base diameter is 90mm, culture dish body (1) wall thickness is 3mm, culture dish body (1) bottom is provided with several culture chambers (3), culture chamber (3) is counterbore, and the spacing of adjacent culture chamber (3) is 0.4cm, is uniformly distributed on culture dish body (1) base plate.

2. a kind of culture of zona-free embryos ware according to claim 1, it is characterized in that: culture chamber (3) degree of depth is 1000um, diameter is 400um.

3. a kind of culture of zona-free embryos ware according to claim 1, is characterized in that: culture dish body (1) bottom is provided with square cultivation region (4), and culture chamber (3) is positioned at cultivation region.