CN204579508U - Paraffin embedding box is used in a kind of Tuna--like Fishes gonadal tissue section - Google Patents

Abstract

The utility model relates to a paraffin embedding box for tuna gonad tissue slices, which belongs to the field of basic scientific research. Its structure includes a box body and upper and lower covers, the box body and the upper and lower covers are made of PVC material, the box body is a cuboid structure, the upper and lower sides of the box body are openings, the box The inner wall of the long side of the body is provided with a plurality of trapezoidal slot holes, and a matching PVC trapezoidal slot sheet is installed on the slot holes, and the upper and lower covers are arranged on the upper and lower sides of the box body. At the openings on both sides, the box body is closed. Because the box body, the upper and lower covers and the slot piece are split structures, the utility model is convenient for replacement, maintenance, removal and cleaning; and each part is made of PVC material, which has a good heat preservation effect and only needs to be preheated together by heating , can be taken out and poured into the wax liquid, the wax block will not cause problems such as splitting and air bubbles due to uneven temperature, which will affect the experimental results.

Description

A kind of paraffin embedding box for tuna gonad tissue section

technical field

The utility model relates to a tissue slicing auxiliary tool used in a laboratory, in particular to a paraffin embedding box used for embedding samples of tuna gonad tissue slices, which belongs to the basic research field of scientific research.

Background technique

Tuna gonad tissue is the main method to study the structural characteristics of tuna tissue, and the microscopic observation of tuna gonad tissue can be effectively carried out by paraffin sectioning and making slices.

Paraffin sectioning is the most widely used method in routine histological preparation techniques. The paraffin section method usually includes steps such as sampling, fixing, washing, dehydration, clearing, paraffin dipping, embedding, sectioning and sticking, dewaxing, staining, dehydration, clearing, and mounting. However, it takes several days from drawing and fixing to sealing to make slide specimens, but the specimens can be stored and used for a long time. They are permanent microscopic slide specimens for long-term use in scientific research.

The above-mentioned embedding is to place the collected tissue samples flatly in the paraffin embedding box according to the required corresponding position according to the requirements of the section, pour the melted paraffin into the embedding box, wait for the paraffin to cool and solidify, take it out, and then Sections were made for microscopic observation. The embedding cassette is an essential tool for tissue section embedding. The existing embedding cassettes are generally composed of a plastic package frame, which is synthesized into a model groove, and then injected with paraffin.

However, during use, since the embedding frame is an integral structure, after pouring the paraffin, the melted paraffin will solidify immediately when it encounters ice cold, and sometimes the embedding frame will crack, affecting the embedding effect, and even the tissue may fall off , The wax block breaks and destroys the tissue block, the wax block and the embedding box are separated, etc., which cause difficulties in the production of slices.

Contents of the invention

The technical problem to be solved by the utility model is to overcome the above-mentioned deficiencies in the prior art, and provide a paraffin embedding box for tuna gonad tissue slices,

In order to solve the technical problems, the technical scheme of the utility model is as follows: a paraffin embedding box for tuna gonad tissue slices, comprising a box body and upper and lower covers, the box body and the upper and lower covers are all made of PVC material Made, the box body is a square frame with a cuboid structure, the upper and lower sides of the box body are openings, the inner wall of the long side of the box body is provided with a plurality of slot holes, and the slot holes are from top to bottom The provided trapezoidal structure is equipped with a matching PVC material trapezoidal slot piece on the slot hole, and the upper and lower covers are arranged on the upper and lower openings of the box body to close the box body.

The long frame of the lower surface of the box body is provided with an elongated groove with a wide top and a narrow bottom. One end of the elongated groove is open and the other end is closed. The lower cover is provided with a flange that matches the elongated groove. The lower cover body is installed as a whole through the cooperation of the flange strip on it and the elongated groove on the box body.

The long frame on the upper surface of the box body is provided with an elongated groove that is wide at the bottom and narrow at the top. One end of the elongated groove is open and the other end is closed. The upper cover is provided with a flange that matches the elongated groove. The above-mentioned upper cover body is installed as a whole through the cooperation of the flange strip on it and the elongated groove on the box body.

A buckle that cooperates with each other is provided between the box body and the lower cover.

There are buckles that cooperate with each other between the box body and the upper cover.

The upper surface of the lower cover is provided with a slot matching the lower end surface of the slot piece.

The inner periphery of the lower part of the box body is provided with an arc-shaped convex wall.

The utility model has beneficial effects. The utility model is mainly used for paraffin embedding of tuna gonad tissue. The box body, upper and lower covers and slot pieces are of split structure, which is convenient for replacement, maintenance and cleaning of components, removal and cleaning.

Moreover, when embedding, due to the split structure of the box body, the lower cover body and the slot piece, after pouring paraffin, the overall structure of the embedding frame is stable and the embedding effect is good.

Moreover, since the box body, upper and lower covers and slot pieces are all made of PVC material, the heat preservation effect is good. You only need to preheat through heating together, then you can take out and pour wax liquid, and the wax block will not be produced due to uneven temperature. Partition, bubbles and other problems, thus affecting the experimental results.

Since the cavity formed by the trapezoidal slot piece can facilitate the complete removal of the wax block, the embedding mold and the embedding block are not easy to separate, the wax block is damaged, and the tissue embedding block needs to be corrected, which is cumbersome. And other issues. The utility model has the advantages of simple structure, low production cost and favorable popularization. The mold can be designed in a variety of specifications and sizes to meet the production of most tissue slices of different sizes.

Description of drawings

Fig. 1 is a schematic diagram of the decomposition structure of the utility model.

Fig. 2 is a schematic diagram of the structure of the box body of the present invention after being cut away.

Fig. 3 is a schematic diagram of the structure of the bottom surface of the upper cover of the utility model.

Fig. 4 is a cross-sectional schematic diagram of the cooperation between the elongated groove on the box body of the utility model and the flange strip on the upper cover.

Detailed ways

The specific structure of the utility model will be described in detail below in conjunction with the accompanying drawings, so that the advantages and features of the utility model can be more easily understood by those skilled in the art.

As shown in FIG. 1 , a paraffin embedding box for tuna gonad tissue slices of the utility model includes a box body 1 , an upper cover body 2 , a lower cover body 3 and a slot piece 4 . The box body 1, upper cover body 2, lower cover body 3, and slot piece 4 are all made of PVC material.

As shown in Figure 2, the box body 1 is a square frame with a cuboid structure, the upper and lower sides of the box body 1 are openings, and the inner wall of the long side of the box body 1 is provided with a plurality of slots 11. The slot 11 is a trapezoidal structure arranged from top to bottom, and a trapezoidal slot piece 4 matching it is installed on the slot 11 . The upper cover body 2 and the lower cover body 3 are arranged on the upper and lower openings of the box body 1 to close the box body, so that the trapezoidal structure cavity formed in this way facilitates the complete removal of the wax block.

The long frame of the lower surface of the box body 1 is provided with an elongated groove (not shown) that is wide at the top and narrow at the bottom. At the beginning, the lower cover body 3 is provided with a flange strip 31 matching with the elongated groove. During installation, the lower cover body 3 is plugged into the elongated groove on the box body 1 from one end through the flange strip 31 on it. Inside, fit and install as a whole. Of course, the box body 1 and the lower cover 3 can also be connected in other ways, for example, between the box body 1 and the lower cover 3 through a snap connection, and a plurality of card slots are arranged on the outer edge of the box body 1 , a plurality of protrusions are distributed on the outer side of the upper surface of the lower cover 3, and the protrusions can be connected into one body as long as they are snapped into the slots during installation.

As shown in Figures 3 and 4, the long frame on the upper surface of the box body 1 is provided with an elongated slot 12 with a lower width and an upper narrow top. One end of the elongated slot 12 is open and the other end is closed. A flange strip 21 matching with the elongated groove is provided, and the upper cover body 2 is installed as a whole by cooperating with the elongated groove 12 on the box body 1 through the flange strip 21 thereon. Like the above connection, the box body 1 and the upper cover 2 can also be connected in other ways, for example, the buckles that cooperate with each other are connected, and a plurality of card slots are arranged on the outer edge of the box body 1. There are a plurality of protrusions distributed at corresponding positions on the outer side of the upper surface of the upper surface. When installing, the protrusions can be connected into one body as long as they are snapped into the card slots.

The upper surface of the lower cover 3 is provided with a slot 32 matching with the lower end surface of the slot piece 4, so that the lower cover 3 and the box body 1 are closely connected.

The inner periphery of the lower part of the box body 1 is provided with an arc-shaped convex wall 13 . The firmness of the box body 1 can be increased to prevent knocking over during operation.

Claims (7)

1. paraffin embedding box is used in a Tuna--like Fishes gonadal tissue section, comprise box body and upper and lower cover body, it is characterized in that: described box body and upper and lower cover body are made by PVC material, described box body is the square frame of rectangular structure, box body upper and lower faces is opening, described box body long limit madial wall is provided with multiple slot hole, described slot hole is the trapezium structure arranged from top to bottom, slot hole is installed on the PVC trapezoidal socket sheet coordinated with it, described upper and lower cover body is arranged on the upper and lower faces opening part on box body, is closed by box body.

2. a kind of Tuna--like Fishes gonadal tissue section paraffin embedding box according to claim 1, it is characterized in that: the lower surface long margin frame of described box body is provided with elongated slot wide at the top and narrow at the bottom, this elongated slot one end open, one end closes, described lower cover is provided with the flange strap coordinated with elongated slot, described lower cover by the flange strap on it coordinate with the elongated slot on box body install in aggregates.

3. a kind of Tuna--like Fishes gonadal tissue section paraffin embedding box according to claim 1 and 2, it is characterized in that: the upper surface long margin frame of described box body is provided with low wide and up narrow elongated slot, this elongated slot one end open, one end closes, described upper cover body is provided with the flange strap coordinated with elongated slot, described upper cover body by the flange strap on it coordinate with the elongated slot on box body install in aggregates.

4. a kind of Tuna--like Fishes gonadal tissue section paraffin embedding box according to claim 1, is characterized in that: be provided with the buckle cooperatively interacted between described box body and lower cover.

5. a kind of Tuna--like Fishes gonadal tissue section paraffin embedding box according to claim 4, is characterized in that: be provided with the buckle cooperatively interacted between described box body and upper cover body.

6. a kind of Tuna--like Fishes gonadal tissue section paraffin embedding box according to claim 1, is characterized in that: the upper surface of described lower cover is provided with the slotted eye matched with slot sheet lower surface.

7. a kind of Tuna--like Fishes gonadal tissue section paraffin embedding box according to claim 1, is characterized in that: described box body lower inside periphery is provided with the convex wall of arc.