CN204237788U - A kind of closed culture dish - Google Patents

Abstract

The utility model discloses a closed culture dish. The closed culture dish comprises a dish bottom and a dish cover which can be fastened tightly with the dish bottom. The dish bottom includes a bottom wall and a side wall of the dish bottom, and the dish cover includes a cover wall. and the side wall of the dish cover, a frictional sealing device is provided on the contact surface of the bottom side wall of the dish and the side wall of the dish cover to limit the airtightness of the contact surface. The cover wall is provided with a through hole or a small hole, and the microporous filter membrane and the hole plug are placed in the through hole, or the filter is placed in the small hole through the apron, and the structure determined accordingly is used as a channel for gas exchange , can maintain the stability of the air composition in the inner cavity of the petri dish, maintain the balance of air pressure inside and outside the petri dish, prevent the bursting of the bottom of the dish and the lid of the dish, and can also filter the air passing through to prevent the growth of pathogenic microorganisms in the petri dish or The passage and diffusion of spores and other harmful gas solutions can ensure the safety of operators.

Description

a closed petri dish

technical field

The utility model relates to an experimental equipment, in particular to a closed culture dish.

Background technique

Petri dishes are one of the most commonly used experimental equipment in biology, and are widely used in the cultivation of microorganisms and the passage of tissue cells. Currently, commonly used petri dishes generally include a dish bottom and a dish cover loosely covering the dish bottom. The inner cavity is used as a culture space. Place the Petri dish in an incubator with adjustable temperature and humidity to establish optimal conditions for culture growth. The air in the incubator has a defined oxygen and carbon dioxide content, and the loosely supported lid allows gas exchange between the chamber cavity and the incubator air. However, when the lid of the dish is opened or the air outside the petri dish flows, it may cause turbulence in the air in the petri dish, and then the aerosol (including mist-like small liquid or small solid particles, etc., such as microorganisms) suspended in the air in the petri dish Bacteria or spores, spores, etc.) are released into the surrounding air, polluting the surrounding environment, which is especially dangerous when cultivating pathogenic microorganisms or exotoxin-producing cells, and users may also suffer health hazards.

CN101875902 B discloses a kind of cell culture dish, comprises a dish bottom and a dish cover that is sealed and arranged on the bottom side wall of the dish, the contact surface of dish cover and dish bottom is a friction locking device, and the sealing state formed can keep the culture dish The inner cavity is sealed. In addition, the petri dish is also provided with a ventilation gap, which can be used as a channel for gas exchange inside and outside the petri dish in a ventilated state. The ventilation state and the sealing state can be conveniently exchanged through manual adjustment to meet the needs of different culture conditions. However, the sealed state and ventilated state of this cell culture dish cannot be switched automatically. When the culture dish is in a sealed state, the exchange of gas inside and outside the culture dish is completely prevented, which may affect the normal growth and reproduction of cultures in the culture dish, especially Aerobic microorganisms. In addition, when the petri dish in a sealed state is used to cultivate some microorganisms with large gas production, the gas generated by the metabolism of the microorganism will gradually increase the air pressure in the petri dish, which will cause the bottom and cover of the dish to burst, and the petri dish The splash of the internal culture or culture medium will cause the failure of culture and extensive pollution.

In addition, some petri dish products use adhesive to detachably bond the dish cover and dish bottom to form an independent and sealed space inside the petri dish. However, after long-term storage, cultivation under different temperature and humidity conditions, or high temperature and high pressure sterilization, the characteristics of the adhesive may change, thereby affecting its sealing effect. The contents of the adhesive may also contaminate the medium and affect the growth of the culture in the dish. Adhesive and sealed petri dishes also prevent the exchange of gas inside and outside the petri dish, and may also affect the growth of aerobic microorganisms in the petri dish or cause the bottom and lid of the dish to burst, and the culture and medium in the petri dish to splash.

Contents of the invention

In view of the above-mentioned shortcomings, the purpose of this utility model is to provide a closed petri dish with simple structure and convenient operation, which can maintain the stability of the air component in the inner chamber of the petri dish, and at the same time, prevent the diffusion of the suspended aerosol in the petri dish, prevent Burst of the bottom and lid of the dish.

For closed culture dish, in order to achieve this purpose, the technical scheme that the utility model adopts is:

A closed petri dish, comprising a dish bottom and a dish cover that can be sealed and fastened with the dish bottom, is characterized in that:

The bottom of the dish includes a bottom wall and at least one side wall of the bottom surrounding and protruding obliquely from the bottom wall, the angle between the bottom wall and the side wall of the bottom of the dish is a first angle, and the first angle is an acute angle;

The lid includes a lid wall and at least one sidewall of the lid surrounding and protruding obliquely from the lid wall, the angle between the lid wall and the side wall of the lid is a second angle, and the second angle is an obtuse angle;

said first angle and said second angle are complementary angles;

On the contact surface between the side wall of the bottom of the dish and the side wall of the lid of the dish, there is a first friction sealing device at the side wall of the bottom of the dish, and a second friction sealing device at the side wall of the lid;

The cover wall is provided with at least one through hole, the through hole does not penetrate the cover wall, and at least one first hole is provided at the bottom of the through hole, and the first hole penetrates the cover wall.

The closed culture dish is characterized in that: it also includes a microporous filter membrane or a hole plug, and the described microporous filter membrane or hole plug is detachably placed in the through hole, and the described hole plug is provided with at least a second pore.

The closed culture dish is characterized in that it further includes at least one first grasping protrusion on the side wall of the bottom of the dish, or at least one second grasping protrusion on the side wall of the lid of the dish.

The closed culture dish is characterized in that it further includes at least one first protrusion located on the bottom wall, or at least one second protrusion located on the cover wall.

The closed culture dish is characterized in that: at least a part of the bottom of the dish or the cover of the dish is made of rigid material, and the rigid material includes glass, synthetic resin or corrosion-resistant metal, etc.; at least a part of the hole plug is made of Composed of materials with certain hardness and elasticity, the material with certain hardness and elasticity is selected from the group consisting of silica gel, rubber or other polymer materials.

A closed petri dish, comprising a dish bottom and a dish cover that can be sealed and fastened with the dish bottom, is characterized in that:

The bottom of the dish includes a bottom wall and at least one side wall of the bottom surrounding and protruding obliquely from the bottom wall, the angle between the bottom wall and the side wall of the bottom of the dish is a first angle, and the first angle is an acute angle;

The lid includes a lid wall and at least one sidewall of the lid surrounding and protruding obliquely from the lid wall, the angle between the lid wall and the side wall of the lid is a second angle, and the second angle is an obtuse angle;

said first angle and said second angle are complementary angles;

On the contact surface between the side wall of the bottom of the dish and the side wall of the lid of the dish, there is a first friction sealing device at the side wall of the bottom of the dish, and a second friction sealing device at the side wall of the lid;

The cover wall includes at least one small hole, and the small hole runs through the cover wall.

The closed culture dish is characterized in that: it also includes an apron or a hole plug or a filter, and the apron or hole plug or filter is detachably placed in the small hole, and the hole plug is set There is at least one second aperture.

The closed culture dish is characterized in that it further includes at least one first grasping protrusion on the side wall of the bottom of the dish, or at least one second grasping protrusion on the side wall of the lid of the dish.

The closed culture dish is characterized in that it further includes at least one first protrusion located on the bottom wall, or at least one second protrusion located on the cover wall.

The closed culture dish is characterized in that: at least a part of the bottom of the dish or the lid of the dish is made of rigid material, and the rigid material includes glass, synthetic resin or corrosion-resistant metal, etc.; At least one part is made of a material with a certain hardness and elasticity, and the material with a certain hardness and elasticity is selected from the group consisting of silica gel, rubber or other polymer material combinations.

Compared with the prior art, the beneficial effects of the utility model are:

After the dish bottom and the dish cover are fastened, the first friction sealing device on the side wall of the dish bottom cooperates with the second friction sealing device on the side wall of the dish cover to define the airtightness of the contact gap between the dish bottom and the dish cover.

The microporous filter membrane is placed in the through hole, and then covered with a hole plug, and the structure determined accordingly has the characteristics of limited air permeability (it can not only maintain the air permeability of the through hole, but also filter the air flowing through): on the one hand, the microporous The pore filter membrane is combined with the first pore of the through hole and the second pore of the plug, which can establish a channel for gas exchange between the gas in the inner cavity of the closed culture dish and the external air, and maintain the stability of the air composition in the inner cavity of the closed culture dish , so the Petri dish will not cause aerobic microorganisms to fail to grow normally due to lack of oxygen. In addition, the existence of the channel keeps the air pressure inside and outside the closed culture dish balanced, which can effectively prevent the bottom and lid of the dish from bursting, as well as the splashing of the culture or culture medium.

On the other hand, the microporous filter membrane in the through hole can filter the air flowing through, preventing the passage of the suspended aerosol in the culture dish. The combination of microporous filter membrane and friction sealing device can effectively prevent the spread and pollution of harmful substances such as bacteria, spores and spores of pathogenic microorganisms in the culture dish, and ensure the safety of operators.

In another technical solution, the filter is tightly placed in the small hole through the apron, which can also achieve the goal of limited ventilation. The filter can maintain the air permeability of the small pores, and can also filter the air passing through, and can also achieve the same filtration and safety protection functions as the microporous membrane.

Description of drawings

In order to illustrate the technical solution of the utility model more clearly, the accompanying drawings that need to be used in the description of the embodiments will be briefly introduced below. Obviously, the accompanying drawings in the following description are only some embodiments of the utility model. For Those of ordinary skill in the art can also obtain other drawings based on these drawings without making creative efforts.

Fig. 1 is the structural representation of the embodiment 1 of closed culture dish;

Fig. 2 is the longitudinal sectional view of embodiment 1;

Fig. 3 is a longitudinal sectional view when a plurality of embodiments 1 are stacked;

Fig. 4 is the partially enlarged sectional view of the through hole of embodiment 1;

Fig. 5 is the schematic plan view of the hole plug of embodiment 1;

Fig. 6 is the longitudinal sectional view of the embodiment 2 of closed culture dish;

Fig. 7 is the partially enlarged cross-sectional view of the filter and aperture of embodiment 2;

Fig. 8 is the schematic plan view of the apron of embodiment 2;

Fig. 9 is a schematic plan view of the hole plug of embodiment 2;

Fig. 10 is the longitudinal sectional view of the embodiment 3 of closed petri dish;

Fig. 11 is the longitudinal sectional view of the embodiment 4 of closed culture dish;

Fig. 12 is a longitudinal sectional view of Example 5 of the closed culture dish.

In the picture:

1—the vessel cover described in Example 1, 2—the cover wall described in Example 1,

3—the side wall of the lid described in embodiment 1, the second friction device described in 4—embodiment 1,

5—the dish bottom described in embodiment 1, the bottom wall described in 6—embodiment 1,

7—the bottom side wall of the dish described in embodiment 1, the first friction device described in 8—embodiment 1,

9—the through hole described in embodiment 1, 10—the second gripping protrusion described in embodiment 1,

11—the first gripping bump described in embodiment 1, 12—the first protrusion described in embodiment 1, 13—the second protrusion described in embodiment 1, 14—in embodiment 1 the first pore,

15—the hole plug described in Example 1, 16—the second aperture described in Example 1,

17—the microporous membrane described in embodiment 1, ∠18—the second angle described in embodiment 1,

∠19—the first angle described in Embodiment 1;

1'--the vessel lid described in embodiment 2, 2'--the cover wall described in embodiment 2,

3'-the side wall of the lid described in embodiment 2, 4'-the second friction device described in embodiment 2,

5'-the bottom of the dish described in embodiment 2, 6'-the bottom wall described in embodiment 2,

7'-the sidewall at the bottom of the dish described in embodiment 2, 8'-the first friction device described in embodiment 2,

9'—the through hole described in embodiment 2, 10'—the second gripping protrusion described in embodiment 2,

11'—the first gripping projection described in Embodiment 2, 12'—the first protrusion described in Embodiment 2,

13'-the second protrusion described in embodiment 2, 15'-the hole plug described in embodiment 2,

16'—the second aperture described in embodiment 2, ∠18'—the second angle described in embodiment 2,

∠19'—the first angle described in embodiment 2, 20'—the filter described in embodiment 2,

21'—the apron described in Example 2;

1 "-the lid of the vessel described in Example 3, 2"-the cover wall described in Example 3,

3"—the side wall of the lid described in Example 3, 5"—the bottom of the dish described in Example 3,

6"—the bottom wall described in Example 3, 7"—the bottom side wall of the dish described in Example 3,

9"—the through hole described in Embodiment 3, ∠18"—the second angle described in Embodiment 3,

∠19"—the first angle described in Embodiment 3;

1'"-the lid of the dish described in Example 4, 5'"-the bottom of the dish described in Example 4,

9'"—the through hole described in Embodiment 4, ∠18'"—the second angle described in Embodiment 4,

∠19'"—the first angle described in Embodiment 4;

1""—the lid of the dish described in Example 5, 5""—the bottom of the dish described in Example 5,

9""—the through hole described in Embodiment 5, ∠18""—the second angle described in Embodiment 5,

∠19""—the first angle described in Example 5.

Detailed ways

In order to make the purpose, technical solutions and advantages of the present utility model clearer, 5 preferred embodiments are exemplified below in conjunction with the accompanying drawings, and the present utility model is further described in detail. The 5 embodiments are as follows: Embodiment 1 , embodiment 2, embodiment 3, embodiment 4, embodiment 5. It should be understood that the described embodiments are only used to explain the utility model, not to limit the utility model. All simplifications or equivalent changes based on the technical solution of the utility model belong to the protection scope of the utility model.

In the discussion of different embodiments, the same symbols are used for the corresponding features, while the different configurations of other embodiments are marked by superscripts, the superscripts (') correspond to Embodiment 2, and the superscripts (") correspond to the embodiment 3. The corner mark ('") corresponds to Example 4, and the corner mark ("") corresponds to Example 5. The shape of the closed petri dish described in the five embodiments is preferably circular, but if you are familiar with the technical field Those skilled in the art will recognize that the invention is also applicable to shapes such as oval, rectangular or other suitable petri dishes.

The utility model discloses a closed culture dish. Figures 1 to 5 show a preferred embodiment 1. The closed culture dish includes a dish bottom 5 and a dish cover 1 capable of sealing and buckling with the dish bottom 5. The bottom of the dish 5 includes a bottom wall 6 and at least one side wall 7 of the bottom of the dish surrounded by the bottom wall 6 and protruding obliquely. The angle between the bottom wall 6 and the side wall of the bottom of the dish 7 is the first angle ∠19, so The aforementioned first angle ∠19 is an acute angle; the described lid 1 includes a lid wall 2 and at least one lid side wall 3 that surrounds and protrudes obliquely from the lid wall 2, and the sandwich between the lid wall 2 and the lid side wall 3 The angle is the second angle ∠18, and the second angle ∠18 is an obtuse angle.

The first angle ∠19 and the second angle ∠18 are supplementary angles to each other. The bottom wall 6 has the same shape as the cover wall 2 and determines the shape of the closed culture vessel. The above two features define that the lid 1 of the dish is tightly fastened with the bottom 5 of the dish.

On the contact surface between the dish bottom side wall 7 and the dish cover side wall 3, the dish bottom side wall 7 has a first friction sealing device 8, and the dish cover side wall 3 has a second friction sealing device 4. The cooperation between the first friction sealing device 8 and the second friction sealing device 4 defines the airtightness of the contact surface between the dish bottom side wall 7 and the dish cover side wall 3 .

The cover wall 2 is provided with at least one through hole 9, the through hole 9 does not penetrate the cover wall 2, and at least one first hole 14 is provided at the bottom of the through hole 9, and the first hole 14 passes through the cover wall2. In addition, the through hole 9 may be formed by recessing the cover wall 2 .

As an improvement, the closed culture dish may also include a microporous filter membrane 17 which is detachably placed in the through hole 9 .

As an improvement, the closed culture dish may further include a detachable hole plug 15 placed in the through hole 9 , and the hole plug 15 has at least one second hole 16 .

Those who are familiar with the technical field should recognize that the through-holes 9, microporous filter membranes 17 or hole plugs 15 involved in the above-mentioned embodiments have the same shape, preferably circular, but the utility model is also applicable to such as elliptical shape, rectangle, or other possible shapes. The diameter of the microporous filter membrane 17 is the same as that of the through hole 9 , while the diameter of the hole plug 15 is slightly larger than that of the through hole 9 . The way the hole plug 15 is placed in the through hole 9 can be in-line or screwed, and other possible ways. The hole plug 15 shown in FIG.

The microporous filter membrane 17 is placed in the through hole 9, and then covered with a hole plug 15 (as shown in Figure 4), the structure established accordingly has the characteristics of limited air permeability, which can maintain the air permeability of the through hole 9, It can also filter the passing air. In this structure, the hole plug 15 with certain elasticity is used to tightly fix the microporous filter membrane 17 , and also can determine the airtightness of the contact surface between the hole plug 15 and the through hole 9 . Therefore, the airflow is limited to a specific channel, that is, the air can only circulate in the airflow channel jointly established by the microporous filter membrane 17 and the first hole 14 of the through hole 9 and the second hole 16 of the hole plug 15 .

The air flow passage is a necessary way for gas exchange inside and outside the closed culture dish, and can maintain the stability of the air composition in the inner cavity of the culture dish, so the culture dish will not prevent aerobic microorganisms from growing normally due to lack of oxygen. In addition, the existence of the airflow channel keeps the air pressure inside and outside the closed culture dish balanced, which can effectively prevent the bottom 5 and the lid 1 of the dish from bursting, and the culture or culture medium from splashing.

On the other hand, the microporous filter membrane 17 in the through hole 9 can filter the air flowing through, preventing the passage of the suspended aerosol in the culture dish. The microporous filter membrane 17 is combined with the first friction sealing device 8 and the second friction sealing device 4, which can effectively prevent the spread and pollution of harmful substances such as bacteria, spores and spores of pathogenic microorganisms in the culture dish, and ensure the safety of operators .

When the microporous filter 17 is not placed in the through hole 9, the air directly flows through the through hole 9 without being filtered by the microporous filter, which can provide a larger air flow for the culture dish to meet the requirements of corresponding culture conditions, such as When using closed culture dishes for growing non-toxic and harmless cells.

Those of ordinary skill in the art should recognize that the microporous membrane 17 can be selected with different pore sizes to meet different requirements, preferably a microporous membrane with a pore size of 0.22 μm.

As an improvement, the closed petri dish may also include at least one first gripping projection 11 located on the bottom side wall 7 of the dish, or at least one second grasping protrusion located on the side wall 3 of the lid 10. The cooperation between the first grasping protrusion 11 and the second grasping protrusion 10 can make the dish cover 1 and the dish bottom 5 more convenient to open or fasten.

As an improvement, the closed culture dish may also include at least one first protrusion 12 located on the bottom wall 6, or at least one second protrusion 13 located on the cover wall 2, the first protrusion 12 has the same shape as the second protrusion 13 . In the embodiment shown in FIG. 3, the diameter of the first protrusion 12 is slightly smaller than that of the second protrusion 13, but those skilled in the art should recognize that in other embodiments the first protrusion The diameter of 12 may also be slightly larger than the size of the second protrusion 13 . The function of the first protrusion 12 and the second protrusion 13 is embodied in that when a plurality of the closed culture dishes are arranged in a stacked structure (as shown in FIG. 3 ), the first protrusion of a culture dish 12 cooperating with a second protrusion 13 of an adjacent Petri dish, it is possible to limit the movement of said one Petri dish relative to said adjacent Petri dish.

At least a part of the dish bottom 5 or the dish cover 1 of the closed culture dish is made of rigid materials, and the rigid materials include glass, synthetic resin or corrosion-resistant metal, etc., preferably rigid, transparent and extinguished by high-pressure steam. Materials that do not deform after sterilization, such as glass, polymer materials, etc.

At least a part of the hole plug 15 of the closed culture dish is made of a material with a certain hardness and elasticity, and the material with a certain hardness and elasticity is selected from the group consisting of silica gel, rubber or other polymer materials.

For the closed culture dish, in another preferred embodiment 2 of the present utility model (as shown in Figures 6 to 9), the closed culture dish includes a dish bottom 5' and can be sealed with the dish bottom 5'. Buckled dish cover 1'; the dish bottom 5' includes a bottom wall 6' and at least one side wall 7' that surrounds and protrudes obliquely from the bottom wall 6', and the bottom wall 6' and the side wall of the dish bottom The included angle of 7' is the first angle ∠19', and the first angle ∠19' is an acute angle; the dish cover 1' includes a cover wall 2' and at least one surrounding and obliquely protruding from the cover wall 2' The lid side wall 3', the angle between the lid wall 2' and the lid side wall 3' is a second angle ∠18', and the second angle ∠18' is an obtuse angle.

The first angle ∠19' and the second angle ∠18' are complementary angles. The bottom wall 6' has the same shape as the cover wall 2', and determines the shape of the closed culture dish simultaneously. The above-mentioned two features limit that the described dish cover 1' is just tightly fastened with the described dish bottom 5'.

On the contact surface between the dish bottom side wall 7' and the dish cover side wall 3', there is a first friction sealing device 8' at the dish bottom side wall 7' and a second friction seal at the dish cover side wall 3'. The device 4', the cooperation between the first friction sealing device 8' and the second friction sealing device 4' defines the airtightness of the contact surface between the side wall 7' of the dish bottom and the side wall 3' of the dish lid.

The cover wall 2' is provided with at least one small hole 9', the small hole 9' does not pass through the cover wall 2', and the small hole 9' can be formed by the depression of the cover wall 2'.

As an improvement, the closed culture dish may also include a detachable hole plug 15' placed in the small hole 9', and the hole plug 15' has at least one second hole 16'.

As an improvement, the closed culture dish may also include a detachable apron 21' or filter 20' placed in the small hole 9', and the apron 21' is used to fix the filter 20 '. The filter 20' can be selected from some mature commercial products, such as the Millex-GP filter and Millex-HV filter produced by Merck Millipore and the Acrodisc filter produced by Caliper Life Science. At the same time, the filter 20' can be selected with different pore size specifications (such as 0.2 μm, 0.22 μm or 0.45 μm, etc.) to meet different requirements.

Those who are familiar with the technical field should recognize that the small hole 9', the apron 21' or the hole plug 15' involved in the above-mentioned embodiment have the same shape, preferably a circular shape, but the utility model is also applicable to such as Oval, rectangular or other possible shapes. The diameter of described apron 21 ' and hole plug 15 ' is slightly greater than the diameter of aperture 9 '. The way the apron 21' and hole plug 15' are placed in the small hole 9' can be in-line or threaded, and other possible ways. The apron 21' shown in Fig. 7 is in-line Placed in the small hole 9'.

When the apron 21' is placed in the small hole 9', and the filter 20' is tightly placed on the apron 21', the apron 21' has a certain elasticity, and the apron 21' and the small hole can be determined. The airtightness of the contact surface of 9' and the airtightness of the contact surface of the apron 21' and the filter 20' limit the air to circulate only through the filter 20'. The filter 20' at this moment also has the feature of limited ventilation, can filter the air flowing through while maintaining the ventilation of the small hole 9', and can also realize the same filtration and safety protection functions as the microporous filter membrane 17.

The filter 20', as a necessary way to exchange gas inside and outside the closed culture dish, can maintain the stability of the air composition in the inner cavity of the culture dish, so the culture dish will not cause aerobic microorganisms to fail to function normally due to lack of oxygen. grow. In addition, the presence of the filter 20' balances the air pressure inside and outside the closed culture dish, which can effectively prevent the bottom 5' of the dish and the lid 1' from bursting, as well as the splashing of the culture or culture medium.

On the other hand, the filter 20' in the aperture 9' can filter the air flowing through, preventing the passage of the suspended aerosol in the petri dish. The filter 20' is combined with the first friction sealing device 8' and the second friction sealing device 4', which can effectively prevent the spread and pollution of harmful substances such as bacteria, spores, and spores of pathogenic microorganisms in the culture dish, and ensure the safety of operators. Safety.

Placing the hole plug 15' in the small hole 9' can prevent large particles of pollutants from passing through the small hole 9', and the second hole 16' positioned at the hole plug 15' can provide a larger space for the culture dish. Air flow to meet the needs of the corresponding culture conditions, such as when using closed culture dishes to cultivate non-toxic and harmless cells.

As an improvement, the closed petri dish may also include at least one first gripping protrusion 11' located on the side wall 7' of the bottom of the dish, or at least one second gripping protrusion located on the side wall 3' of the lid. Holding bump 10'. The cooperation between the first grasping protrusion 11' and the second grasping protrusion 10' can make the described dish cover 1' and the dish bottom 5' more convenient to open or fasten.

As an improvement, the closed culture dish may also include at least one first protrusion 12' located on the bottom wall 6', or at least one second protrusion 13' located on the cover wall 2'. The shape of the first protrusion 12' is the same as that of the second protrusion 13', and its function is reflected in that when a plurality of the closed petri dishes are arranged in a stacked structure, the first protrusion of a petri dish The protrusion 12' cooperating with the second protrusion 13' of the adjacent Petri dish makes it possible to limit the movement of said one Petri dish relative to said adjacent Petri dish.

At least a part of the dish bottom 5' or the dish cover 1' of the closed culture dish is made of rigid materials, and the rigid materials include glass, synthetic resin or corrosion-resistant metal, etc., preferably rigid, transparent and high-pressured. Materials that do not deform after steam sterilization, such as glass, polymeric materials, etc.

At least a part of the apron 21' or the hole plug 15' of the closed culture dish is made of a material with a certain hardness and elasticity, and the material with a certain hardness and elasticity is selected from the combination of silica gel, rubber or other polymer materials of groups.

In another preferred embodiment 3 of the closed petri dish (as shown in FIG. 10 ), the bottom wall 6'' and the side wall 7'' of the dish bottom form a right angle inside the petri dish, An acute angle is formed on the outside (that is, the first angle ∠19''); the cover wall 2'' and the side wall of the dish cover 3'' form a right angle on the outside of the culture dish and an obtuse angle on the inside (That is, the second angle ∠18''). The first angle ∠19'' and the second angle ∠18'' are complementary angles. The shape of the closed petri dish described in Example 3 is flat, which facilitates the grasping of the bottom 5'' and the dish cover 1'', and can increase the stability when a plurality of the closed petri dishes are arranged in a stacked structure . The inner cavity defined by the bottom wall 6'' and the dish bottom side wall 7'' is cylindrical, which is more convenient for experimental operation or result observation.

For the closed culture dish, embodiment 4 (as shown in FIG. 11 ) and embodiment 5 (as shown in FIG. 12 ) are variations of embodiment 3. The structure and characteristics of the dish cover 1''' described in embodiment 4 are equal to the dish cover 1 described in embodiment 1, and the structure and characteristics of the described dish bottom 5''' are equal to those described in embodiment 3 The bottom of the dish is 5''. The structure and characteristics of the dish cover 1'''' described in Example 5 are equal to the dish cover 1'' described in Example 3, and the structure and characteristics of the described dish bottom 5'''' are equal to those of the embodiment Dish base 5 as described in Example 1.

The structure and characteristics of the through holes (including through hole 9'', through hole 9''' and through hole 9'''') shown in Fig. 10, Fig. 11 and Fig. 12 are the same as the through holes described in Embodiment 1 9, but it should be understood that the through hole 9'' or the through hole 9''' or the through hole 9'''' can also be designed to be similar to the structure and characteristics of the small hole 9' described in Embodiment 2 .

For Embodiment 3, Embodiment 4 or Embodiment 5, except for the above features, all other structures and features are similar to Embodiment 1 or Embodiment 2. Embodiment 3, embodiment 4 or embodiment 5 can all achieve the same beneficial effect as embodiment 1 or embodiment 2.

Through the introduction to the listed embodiments above, the basic idea and basic principle of the present utility model have been set forth. Apparently, the described embodiments are only part of the embodiments of the present invention, and are not exhaustive. Based on the embodiments of the present utility model, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present utility model.

Claims (10)

1. A closed petri dish, comprising the bottom of the dish and the dish cover that can be sealed and fastened with the bottom of the dish, is characterized in that: The bottom of the dish includes a bottom wall and at least one side wall of the bottom surrounding and protruding obliquely from the bottom wall, the angle between the bottom wall and the side wall of the bottom of the dish is a first angle, and the first angle is an acute angle; The lid includes a lid wall and at least one sidewall of the lid surrounding and protruding obliquely from the lid wall, the angle between the lid wall and the side wall of the lid is a second angle, and the second angle is an obtuse angle; said first angle and said second angle are complementary angles; On the contact surface between the side wall of the bottom of the dish and the side wall of the lid of the dish, there is a first friction sealing device at the side wall of the bottom of the dish, and a second friction sealing device at the side wall of the lid; The cover wall is provided with at least one through hole, the through hole does not penetrate the cover wall, and at least one first hole is provided at the bottom of the through hole, and the first hole penetrates the cover wall. 2. The closed culture dish according to claim 1, characterized in that: it also includes a microporous filter membrane or a plug, and the described microporous filter membrane or plug is detachably placed in the through hole , the hole plug is provided with at least one second hole. 3. The closed petri dish according to claim 1, characterized in that: it also comprises at least one first gripping protrusion on the side wall of the bottom of the dish, or at least one second gripping protrusion on the side wall of the lid of the dish. Grab the bumps. 4. The closed culture dish according to claim 1, further comprising at least one first protrusion on the bottom wall, or at least one second protrusion on the cover wall. 5. The closed culture dish according to claim 1 or 2, characterized in that: at least a part of the bottom of the dish or the lid of the dish is made of a rigid material, and the rigid material includes glass, synthetic resin or corrosion-resistant Metal, etc.; at least a part of the hole plug is made of a material with a certain hardness and elasticity, and the material with a certain hardness and elasticity is selected from the group consisting of silica gel, rubber or other polymer material combinations. 6. A closed petri dish, comprising a dish bottom and a dish cover that can be sealed and fastened with the bottom of the dish, characterized in that: The bottom of the dish includes a bottom wall and at least one side wall of the bottom surrounding and protruding obliquely from the bottom wall, the angle between the bottom wall and the side wall of the bottom of the dish is a first angle, and the first angle is an acute angle; The lid includes a lid wall and at least one sidewall of the lid surrounding and protruding obliquely from the lid wall, the angle between the lid wall and the side wall of the lid is a second angle, and the second angle is an obtuse angle; said first angle and said second angle are complementary angles; On the contact surface between the side wall of the bottom of the dish and the side wall of the lid of the dish, there is a first friction sealing device at the side wall of the bottom of the dish, and a second friction sealing device at the side wall of the lid; The cover wall includes at least one small hole, and the small hole runs through the cover wall. 7. The closed culture dish according to claim 6, characterized in that: it also includes an apron or a plug or a filter, and the apron or a plug or a filter is detachably placed in the small In the hole, the hole plug is provided with at least one second hole. 8. The closed culture dish according to claim 6, characterized in that: it also comprises at least one first gripping protrusion on the side wall of the bottom of the dish, or at least one second gripping protrusion on the side wall of the lid of the dish. Grab the bumps. 9. The closed culture dish according to claim 6, further comprising at least one first protrusion on the bottom wall, or at least one second protrusion on the cover wall. 10. The closed culture dish according to claim 6 or 7, characterized in that: at least a part of the bottom of the dish or the lid of the dish is made of rigid material, and the rigid material includes glass, synthetic resin or corrosion-resistant Metal, etc.; at least a part of the hole plug is made of a material with a certain hardness and elasticity, and the material with a certain hardness and elasticity is selected from the group consisting of silica gel, rubber or other polymer material combinations.