CN204174219U - A kind of cell in vitro sterile culture device - Google Patents

Abstract

The utility model discloses an in vitro cell aseptic culture device, which is composed of an upper cover assembly, a box body assembly, a hinge, a square water box, a square inner box assembly, and a square cell-carrying container frame assembly. The two upper cover assemblies can be opened and closed connected with the box assembly through hinges respectively. When connected, the return-shaped sealing gaskets at the lower ends of the two upper cover assemblies are respectively sealed and connected with the two openings around the two openings on the box assembly with double-opening cover plates. , in the left and right inner cavities separated by two neutral plates in the inner frame of the box assembly, the square inner box assembly is connected through the upper annular partition, the lower annular partition, and the Tian-shaped partition respectively. A square water box is placed in the square inner box assembly and is connected with a square cell-carrying container frame assembly through its support plate. The left and right two square inner box components can be used alternately in the cell culture box for a long time, and the ultraviolet lamp is sterilized, which can completely avoid the bacterial contamination of the cells. It is a relatively simple structure, economical and practical in vitro cell culture device.

Description

An in vitro cell sterile culture device

technical field

The utility model relates to an in vitro cell aseptic culture device, which belongs to the technical field of medical and sanitary equipment.

Background technique

Cell culture is one of the important research methods in medical scientific research experiments. In the process of cell culture, the requirements for temperature, humidity, carbon dioxide concentration, and especially the sterility are very high, so the requirements for cell culture equipment are relatively high. Generally, imported cell culture devices are expensive, which is not conducive to the popularization of small and medium-sized laboratories. Most of the domestic devices use external heating and only have a single box structure. When the cell culture time is long, the cells are easily polluted in the box and bacteria breed, which leads to cell culture. fail.

Contents of the invention

In order to solve the above problems, the purpose of this utility model is to provide an in vitro cell aseptic culture device, which is provided with two inner cavities, left and right, equipped with two sets of identical square inner box assemblies and square water boxes and through The carbon dioxide concentration controller, temperature controller, and ultraviolet lamp controller independently control the carbon dioxide gas outlet control valve, ceramic heating plate, and ultraviolet lamp. All five surfaces are heated by a ceramic heating plate, which has the characteristics of fast temperature rise and quick response. The square water box with a large opening has sufficient water vapor emission, and the humidity requirement can be met without control. Two inner boxes, left and right, are used. , can be used as an inner box for culturing cells, and can be sterilized in a separate box. When cell culture is required for a long time, it can be cultured in the left box first, and after a certain period of time, the square cell-carrying container rack assembly together with the cell-carrying container is quickly transferred to the right box for cell culture. The lamp is sterilized to ensure continuous aseptic culture, and the ultraviolet lamp has no effect on the cultured cells. After the cells are transferred out of the left box, turn on the ultraviolet light to sterilize and prepare for rotation. In this way, the two boxes are sterilized alternately by ultraviolet light within a certain period of time, which will not affect the normal growth of the cells, and can completely avoid the occurrence of abnormalities caused by long-term cell culture. Cell contamination, bacteria breeding and other conditions occur.

The technical solution adopted by the utility model to solve the technical problem is: an in vitro cell aseptic culture device, which is composed of an upper cover assembly, a box body assembly, a hinge, a square water box, a square inner box assembly, and a square cell-carrying container. frame components. The two upper cover assemblies can be opened and closed connected with the box assembly through hinges respectively. When connected, the return-shaped sealing gaskets at the lower ends of the two upper cover assemblies are respectively sealed and connected with the two openings around the two openings on the box assembly with double-opening cover plates. , in the left and right inner cavities separated by two neutral plates in the inner frame of the box assembly, the square inner box assembly is connected through the upper annular partition, the lower annular partition, and the Tian-shaped partition respectively. A square water box is placed in the square inner box assembly and is connected with a square cell-carrying container frame assembly through its support plate. The upper cover assembly is composed of an outer concave groove plate, an inner concave groove plate, a back-shaped edge banding plate, a back-shaped gasket, a U-shaped handle, and an ultraviolet lamp. The edge banding plate is connected to the lower periphery of the inner concave groove plate, and a thermal insulation material layer is connected between the outer concave groove plate and the inner concave groove plate, and a back-shaped sealing gasket is connected to the back-shaped edge banding plate, and a back-shaped gasket is connected to the outer concave groove plate. A U-shaped handle is fixed on the front of the slot plate, and an ultraviolet lamp is fixed in the inner concave slot plate. The box assembly is composed of an outer frame coaming, an inner frame coaming, a left side vertical plate, a right side vertical plate, a middle plate, a cover plate with double openings, a bottom plate, a support, an upper annular partition, a lower annular partition Plate, Tian-shaped partition, bottom partition, frame plate, glass, carbon dioxide concentration controller, temperature controller, ultraviolet lamp controller, rectangular ceramic heating plate, square ceramic heating plate, carbon dioxide concentration sensor, temperature sensor, carbon dioxide intake The mouth assembly is composed of an inner frame coaming placed inside the outer frame coaming, a left vertical plate and a right vertical vertical plate are respectively connected between the outer frame coaming and the inner frame coaming, and two In the neutral plate, a layer of thermal insulation material is connected between the two neutral plates, and the outer periphery of the inner frame coaming is connected with a layer of thermal insulation material through the outer frame coaming, the left vertical plate, and the right vertical plate, and the outer frame coaming and inner frame The bottom of the coaming is connected with the bottom plate, and the support is connected under the bottom of the coaming. The space formed with the bottom plate is connected with a thermal insulation material layer, and a cover plate with double openings is connected on the outer frame coaming plate and the inner frame coaming plate. correspond. A front opening is correspondingly provided on the front upper half of the outer frame coaming board and the inner frame coaming board, frame plates are connected to the inner peripheries of the two front openings, and double-layer glass is connected to the frame boards. Both left and right inner cavities are horizontally connected with an upper annular partition and a lower annular partition. Rectangular ceramic heating plates are respectively fixed at the back, and rectangular ceramic heating plates are respectively fixed on the inner frame enclosure between the lower annular partition and the bottom partition and the front, rear, left and right sides of the inner side of the neutral board, and on the bottom partition A cross-shaped partition is connected, and a square ceramic heating plate is respectively fixed in the cross-shaped partition. Behind the outer frame coaming board and through the inner frame coaming board and insulation material layer, there are two carbon dioxide intake nozzle assemblies sealed and connected. The roads are respectively connected with the carbon dioxide outlet control valve, and the two sides of the left and right inner chambers are respectively fixed with carbon dioxide concentration sensors and temperature sensors, and the two sides in front of the outer frame panel are respectively connected with carbon dioxide concentration controllers, Temperature controller, ultraviolet lamp controller, the carbon dioxide concentration controllers on the left and right sides are respectively connected with the carbon dioxide concentration sensors and the corresponding carbon dioxide gas outlet control valves in the left and right inner chambers, and the carbon dioxide concentration controllers on the left and right sides The temperature controllers are respectively connected with the temperature sensors of the left and right inner chambers, the rectangular ceramic heating plate, and the square ceramic heating plate, and the ultraviolet lamp controllers on the left and right sides are respectively fixed in the left and right inner concave groove plates. connected to the UV lamp. The square inner box assembly is composed of an inner box main body, a support plate, and a transparent plate. The inner box main body is a square structure, and its upper end is an opening folded inward. The two sides of the inner box main body and the rear middle The upper part is connected with a support plate, and a transparent plate is connected with the upper opening in front of the main body of the inner box. The square cell-carrying container rack assembly is composed of an upper shelf plate, a lower shelf plate, square pillars, and a cell-carrying container. The upper shelf plate is connected to the lower shelf plate through four square pillars, and container holes are arranged on the upper shelf plate. The bottom hole of the container is arranged on the lower shelf plate, and a plurality of through holes are also arranged on the lower shelf plate. A cell-carrying container is placed in the container hole of the upper shelf plate, and the lower end of the cell-carrying container is connected with the bottom hole of the container. the

The beneficial effects of the utility model are: adopting the left and right square inner box components, both can be used as a cell culture box, and the inner box is provided with an ultraviolet lamp, which is controlled and irradiated by an ultraviolet lamp for a certain period of time before use. When cells need to be cultivated for a long time, they can be cultivated in the left box first, and then transferred to the right box for further cultivation after a certain period of time. Alternately use ultraviolet light to sterilize, which can completely avoid cell bacterial contamination and can effectively sterilize Sterilization. The transparent glass for observation is arranged on the box body and the square liner assembly, which is a relatively simple, economical and practical in vitro cell sterile culture device.

Description of drawings

The utility model will be further described below in conjunction with the accompanying drawings and specific embodiments.

Fig. 1 is a schematic structural view of an in vitro cell aseptic culture device of the present invention.

FIG. 2 is a schematic cross-sectional structure diagram of FIG. 1 .

FIG. 3 is a schematic top view of the structure in FIG. 1 .

Fig. 4 is a schematic diagram of the sectional structure along line A-A of Fig. 2 .

Fig. 5 is a schematic diagram of the sectional structure along line BB of Fig. 2 .

Fig. 6 is a schematic cross-sectional structural diagram of CC in Fig. 2 .

Fig. 7 is a schematic structural diagram of the square inner box assembly in Fig. 1 .

FIG. 8 is a schematic top view of the structure of FIG. 7 .

FIG. 9 is a schematic structural view of the square cell-carrying container rack assembly in FIG. 1 .

FIG. 10 is a schematic top view of the structure of FIG. 9 .

Fig. 11 is a schematic diagram of the sectional structure along line D-D in Fig. 9 .

reference sign

1. Upper cover assembly 2. Box body assembly 3. Hinge 4. Square water box 5. Square inner box assembly

6. Square cell container rack assembly

11. Outer concave groove plate 12. Inner concave groove plate 13. Back-shaped edge banding plate 14. Back-shaped gasket

15. U-shaped handle 16. Ultraviolet lamp

21. Outer frame panel 22. Inner frame panel 23. Left vertical panel 24. Right vertical panel

25. Neutral plate 26. Cover plate with double openings 27. Bottom plate 28. Support 29. Upper annular partition 30. Lower annular partition 31. Tian-shaped partition 32. Bottom partition 33. Frame board

34. Glass 35. Carbon dioxide concentration controller 36. Temperature controller

37. Ultraviolet lamp controller 38. Rectangular ceramic heating plate 39. Square ceramic heating plate

40. Carbon dioxide concentration sensor 41. Temperature sensor 42. Carbon dioxide intake nozzle assembly

51. Main body of inner box 52. Support board 53. Transparent board

61. Upper shelf plate 62. Lower shelf plate 63. Square pillar 64. Container hole 65. Container bottom hole

66, through hole 67, contain cell container.

Detailed ways

Please refer to Figures 1 to 11, an in vitro cell aseptic culture device, which is composed of an upper cover assembly, a box body assembly, a hinge, a square water box, a square inner box assembly, and a square cell-carrying container frame assembly. The two upper cover assemblies 1 are respectively openably and closably connected to the box assembly 2 through hinges 3 , and when connected, the return-shaped gaskets 14 at the lower ends of the two upper cover assemblies 1 are respectively connected to the cover plate 26 with double openings on the box assembly 2 . The two openings are sealed and connected around, and in the left and right inner cavities separated by two neutral plates 25 in the inner frame coaming plate 22 of the box assembly 2, the upper annular partition 29 and the lower annular partition 30 are passed through respectively. The square-shaped partition 31 is connected with a square inner box assembly 5, and a square water box 4 is placed in the square inner box assembly 5 and a square cell-containing container rack assembly 6 is connected with a support plate 52 thereof. The upper cover assembly 1 is composed of an outer concave groove plate 11, an inner concave groove plate 12, a back-shaped edge banding plate 13, a back-shaped gasket 14, a U-shaped handle 15, and an ultraviolet lamp 16. The lower periphery of the plate 11 is connected to the lower periphery of the inner concave groove plate 12 through the back-shaped edge banding plate 13, and a layer of thermal insulation material is connected between the outer concave groove plate 11 and the inner concave groove plate 12. The plate 13 is connected with a back-shaped gasket 14, a U-shaped handle 15 is fixed in front of the outer concave groove plate 11, and an ultraviolet lamp 16 is fixed in the inner concave groove plate 12. The box assembly 2 is composed of an outer frame coaming plate 21, an inner frame coaming plate 22, a left vertical plate 23, a right vertical plate 24, a middle plate 25, a double opening cover plate 26, a bottom plate 27, and a support 28. , upper annular partition 29, lower annular partition 30, Tian-shaped partition 31, bottom partition 32, frame plate 33, glass 34, carbon dioxide concentration controller 35, temperature controller 36, ultraviolet lamp controller 37, rectangular ceramics Heating plate 38, square ceramic heating plate 39, carbon dioxide concentration sensor 40, temperature sensor 41, carbon dioxide inlet nozzle assembly 42 constitute, and inner frame coaming plate 22 is placed in outer frame coaming plate 21, and outer frame coaming plate 21 and inner frame encircle The left side vertical board 23 and the right side vertical board 24 are respectively connected between the boards 22, two neutral boards 25 are connected in the middle of the inner frame coaming board 22, and an insulating material layer is connected between the two neutral boards 25. The outer periphery of the frame coaming board 22 is connected with an insulating material layer through the outer frame coaming board 21, the left side vertical board 23, and the right side vertical board 24, and a bottom plate 27 is connected below the outer frame coaming board 21 and the inner frame coaming board 22, and the bottom board 27 Connected with a support 28, the lower sides of the left and right inner cavities separated by two neutral plates 25 in the inner frame coaming plate 22 are respectively connected with a bottom partition 32, and the bottom partition 32 and the bottom plate 27 form The space is connected with a thermal insulation material layer, and a cover plate 26 with double openings is connected on the outer frame coaming plate 21 and the inner frame coaming plate 22, and the two openings on the cover plate 26 with double openings are connected with the left and right inner cavities respectively. correspond. Front openings are correspondingly arranged on the front upper half of the outer frame coaming plate 21 and the inner frame coaming plate 22 , frame plates 33 are connected to the inner peripheries of the two front openings, and double-layer glass 34 is connected in the frame plates 33 . In the left and right inner cavities, there are upper annular partitions 29 and lower annular partitions 30 connected transversely. Rectangular ceramic heating plates 38 are respectively fixed on the left, right and rear sides of the inside, and are respectively fixed on the front, rear, left and right sides of the inner frame coaming plate 22 between the lower annular partition plate 30 and the bottom surface partition plate 32 and the inside of the neutral plate 25. Rectangular ceramic heating plate 38 is arranged, and on the bottom surface dividing plate 32, be connected with square-shaped dividing plate 31, in its square-shaped dividing plate 31, be respectively fixed with square ceramic heating plate 39. Behind the outer frame coaming board 21 and through the inner frame coaming board 22 and the insulation material layer, there are two carbon dioxide intake nozzle assemblies 42 sealed and connected. The inner ends of the carbon dioxide intake nozzle assemblies 42 extend into the left and right inner chambers respectively. The outer ends are respectively connected to the carbon dioxide gas outlet control valves through pipelines. The carbon dioxide concentration sensor 40 and the temperature sensor 41 are respectively fixed on both sides of the left and right inner chambers. Connected with a carbon dioxide concentration controller 35, a temperature controller 36, and an ultraviolet lamp controller 37, the carbon dioxide concentration controller 35 on its left and right sides is connected with the carbon dioxide concentration sensors 40 and the corresponding carbon dioxide concentration sensors of the left and right inner chambers respectively. The air outlet control valve is connected, and the temperature controller 36 on its left and right sides is connected with the temperature sensor 41, the rectangular ceramic heating plate 38, and the square ceramic heating plate 39 of the left and right inner cavities respectively. The ultraviolet lamp controller 37 is respectively connected with the ultraviolet lamps 16 fixed in the left and right inner concave groove plates 12 . The square inner box assembly 5 is composed of an inner box main body 51, a support plate 52, and a transparent plate 53. The inner box main body 51 is a square structure, and its upper end is an opening folded inward. The middle and upper part of each side and the back is connected with support plate 52, is connected with transparent plate 53 on the opening of inner box main body 51 front middle upper part, and the position of its opening is in the front upper half of outer frame coaming plate 21 and inner frame coaming plate 22. Correspondingly, a front opening is provided correspondingly. The described square cell-containing container rack assembly 6 is composed of an upper shelf plate 61, a lower shelf plate 62, a square pillar 63, and a cell-containing container 67. The upper shelf plate 61 is connected with the lower shelf plate 62 by four square pillars 63, and the upper shelf plate 61 is provided with a container hole 64, the corresponding container hole 64 is provided with a container bottom hole 65 on the lower shelf plate 62, and a plurality of through holes 66 are also provided on the lower shelf plate 62, and a load is placed in the container hole 64 of the upper shelf plate 61. The cell container 67, the lower end of the cell container 67 is connected with the bottom hole 65 of the container.

Claims (5)

1. An in vitro cell aseptic culture device, which is composed of an upper cover assembly, a casing assembly, a hinge, a square water box, a square inner box assembly, and a square cell-carrying container frame assembly, and is characterized in that: two upper covers The components (1) can be opened and closed with the box component (2) through the hinges (3) respectively. The two openings with double-opening cover plates (26) on the top are sealed and connected around, and the left and right two sides are separated by two neutral plates (25) in the inner frame coaming plate (22) of the box assembly (2). The inner cavity is connected with a square inner box assembly (5) through an upper annular partition (29), a lower annular partition (30), and a square-shaped partition (31), and a square inner box assembly (5) is placed inside the square inner box assembly (5). The water box (4) is connected with a square cell-carrying container frame assembly (6) through its support plate (52). 2. An in vitro cell sterile culture device according to claim 1, characterized in that: the upper cover assembly (1) is composed of an outer concave groove plate (11), an inner concave groove plate (12) , back-shaped edge banding plate (13), back-shaped sealing gasket (14), U-shaped handle (15), ultraviolet lamp (16), and the lower periphery of the outer concave groove plate (11) passes through the back-shaped edge banding plate ( 13) It is connected with the lower periphery of the inner concave groove plate (12), and there is a layer of thermal insulation material connected between the outer concave groove plate (11) and the inner concave groove plate (12). Be connected with back-shaped gasket (14) on the top, be fixed with U-shaped handle (15) in front of outer concave groove plate (11), be fixed with ultraviolet light (16) in inner concave groove plate (12). 3. An in vitro cell sterile culture device according to claim 1, characterized in that: the box assembly (2) is composed of an outer frame coaming (21), an inner frame coaming (22), a left Side vertical plate (23), right vertical plate (24), middle plate (25), cover plate with double openings (26), bottom plate (27), support (28), upper annular partition (29), lower Annular partition (30), Tian-shaped partition (31), bottom partition (32), frame plate (33), glass (34), carbon dioxide concentration controller (35), temperature controller (36), ultraviolet lamp A controller (37), a rectangular ceramic heating plate (38), a square ceramic heating plate (39), a carbon dioxide concentration sensor (40), a temperature sensor (41), and a carbon dioxide inlet nozzle assembly (42) are formed. 21) The inner frame coaming (22) is placed inside, and the left vertical plate (23) and the right vertical plate (24) are respectively connected between the outer frame coaming (21) and the inner frame coaming (22). The middle part of the inner frame coaming board (22) is connected with two neutral boards (25), between the two neutral boards (25) is connected with a thermal insulation material layer, and the outer circumference of the inner frame coaming board (22) passes through the outer frame coaming board (21 ), the left vertical plate (23), the right vertical plate (24) are connected with insulation material layers, and the bottom plate (27) is connected with the outer frame coaming plate (21) and the inner frame coaming plate (22), and the bottom plate (27 ) is connected with a support (28) below, and the lower sides of the left and right inner cavities separated by two neutral plates (25) in the inner frame coaming (22) are respectively connected with a bottom partition (32), In the space formed by the bottom surface partition (32) and the bottom plate (27), a thermal insulation material layer is connected, and a cover plate (26) with double openings is connected on the outer frame coaming (21) and the inner frame coaming (22). The two openings on the double-opening cover plate (26) correspond to the left and right inner cavities respectively; the upper half of the front of the outer frame coaming (21) and the inner frame coaming (22) are correspondingly provided with a front opening, and the The inner peripheries of the two front openings are connected with frame plates (33), and double-layer glass (34) is connected in the frame plates (33); the upper annular partitions (29) are transversely connected in the left and right inner cavities. ), the lower annular partition (30), on the inner frame coaming (22) between the upper annular partition (29) and the lower annular partition (30) and on the left, right and rear sides of the neutral plate (25) The rectangular ceramic heating plate (38) is respectively fixed, the front, rear, left and right sides of the inner frame enclosure (22) between the lower annular partition (30) and the bottom partition (32) and the inner side of the neutral board (25) Rectangular ceramic heating plate (38) is respectively fixed on the bottom surface dividing plate (32) and is connected with Tian-shaped dividing plate (31), and square ceramic heating plate (39) is respectively fixed in its Tian-shaped dividing plate (31); Behind the frame coaming (21) and through the inner frame coaming (22) and thermal insulation material layer, there are two carbon dioxide inlet nozzle assemblies (42), and the inner ends of the carbon dioxide inlet nozzle assemblies (42) extend into the left and right sides respectively. In the two lumens, the outer ends are respectively connected with the dioxygen through the pipeline The carbon dioxide gas outlet control valve is connected, and a carbon dioxide concentration sensor (40) and a temperature sensor (41) are respectively fixed on both sides of the left and right inner chambers, and the two sides in front of the outer frame panel (21) are respectively fixed from top to bottom. It is connected with a carbon dioxide concentration controller (35), a temperature controller (36), and an ultraviolet lamp controller (37). The concentration sensor (40) is connected with the corresponding carbon dioxide gas outlet control valve, and the temperature controllers (36) on the left and right sides are respectively connected with the temperature sensors (41) and the rectangular ceramic heating plate (38) of the left and right inner chambers. ), the square ceramic heating plate (39) is connected, and the ultraviolet lamp controller (37) on its left and right sides is respectively connected with the ultraviolet lamp (16) fixed in the left and right inner concave groove plate (12). 4. An in vitro cell sterile culture device according to claim 1, characterized in that: the square inner box assembly (5) is composed of an inner box body (51), a support plate (52), a transparent plate ( 53), the main body of the inner box (51) is a square structure, and its upper end is an opening folded inward, and the two sides of the main body of the inner box (51) and the middle and upper part of the back are connected with a support plate (52). A transparent plate (53) is connected to the opening at the middle and upper part of the front of the inner box main body (51). correspond. 5. An in vitro cell aseptic culture device according to claim 1, characterized in that: said square cell-carrying container rack assembly (6) is composed of an upper shelf plate (61), a lower shelf plate (62), a square Consists of pillars (63) and cell-carrying containers (67), the upper shelf plate (61) is connected with the lower shelf plate (62) through four square pillars (63), and the upper shelf plate (61) is provided with container holes (64), Corresponding container hole (64) is provided with container bottom hole (65) on lower shelf plate (62), is also provided with a plurality of through holes (66) on lower shelf plate (62), in the container hole (61) of upper shelf plate (61) 64) A cell-carrying container (67) is placed inside, and the lower end of the cell-carrying container (67) is connected to the bottom hole (65) of the container.