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CN211814437U - an incubator - Google Patents

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CN211814437U
CN211814437U CN202020278199.5U CN202020278199U CN211814437U CN 211814437 U CN211814437 U CN 211814437U CN 202020278199 U CN202020278199 U CN 202020278199U CN 211814437 U CN211814437 U CN 211814437U
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incubator
pipeline
compartment
gas
box
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李蓉
杜宇娜
张辉青
陈益国
高婧
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Jiangxi Jinyu Medical Laboratory Co ltd
Jiangxi Provincial Peoples Hospital
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Jiangxi Kingmed Diagnostics Inspection Institute Co ltd
Jiangxi Provincial Peoples Hospital
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Abstract

本实用新型公开了一种培养箱,包括箱体和外箱门,其特征在于,所述外箱门的一侧与箱体通过铰接的方式活动连接,所述箱体内设有多个隔热板层,每个隔热板层将箱体划分成多个隔间,每个隔间上还配备有内箱门,所述箱体上还安装有开关,每个所述隔间上还配备有管道系统,所述管道系统包括输送消毒气体管道、抽出培养箱内气体管道、输送二氧化碳管道、输送氧气管道和气体流通控制装置,每个所述隔间内还安装有紫外线灭菌灯、摄像头和监测设备,所述开关、管道系统、紫外线灭菌灯、摄像头和监测设备分别与控制器电性连接。本实用新型解决了现有技术微生物培养箱通用性不强,且易发生交叉污染的问题。

Figure 202020278199

The utility model discloses an incubator, comprising a box body and an outer box door. The utility model is characterized in that one side of the outer box door is movably connected with the box body by means of hinges, and a plurality of partitions are arranged in the box body. Heat plate layer, each heat insulation plate layer divides the box into a plurality of compartments, each compartment is also equipped with an inner box door, a switch is also installed on the box body, and each compartment is also Equipped with a pipeline system, the pipeline system includes a pipeline for conveying sterilized gas, a pipeline for extracting gas in the incubator, a pipeline for conveying carbon dioxide, a pipeline for conveying oxygen and a gas circulation control device, and each compartment is also equipped with an ultraviolet sterilization lamp, A camera and monitoring equipment, the switch, the piping system, the ultraviolet sterilization lamp, the camera and the monitoring equipment are respectively electrically connected with the controller. The utility model solves the problems that the prior art microbial incubator is not versatile and prone to cross-contamination.

Figure 202020278199

Description

一种培养箱an incubator

技术领域technical field

本实用新型涉及微生物培养技术领域,具体为一种培养箱。The utility model relates to the technical field of microorganism culture, in particular to an incubator.

背景技术Background technique

目前常用于临床的微生物培养箱多为CO2恒温培养箱,结构为立式箱体,内门用钢化玻璃制成。内室放置用于承托培养物的不锈钢隔板,可方便移动,并可任意改变高度。工作室和玻璃门之间装有硅橡胶密封圈,温度控制一般为5-50℃,带有温度指示、CO2浓度指示等装置。常用于微生物培养箱杀菌的技术为紫外灯照射,如将培养箱打开后开启房内紫外灯进行照射,或在培养箱内部安装紫外灯装置进行照射。通常一个培养箱内整体环境不可分隔,只能用于培养在一种特定环境下生长繁殖的微生物。At present, most of the microbial incubators commonly used in clinical are CO2 constant temperature incubators, which are vertical boxes and the inner door is made of tempered glass. The inner chamber is placed with a stainless steel partition for supporting the culture, which can be easily moved and the height can be changed arbitrarily. A silicone rubber sealing ring is installed between the studio and the glass door, and the temperature control is generally 5-50 ℃, with temperature indication, CO2 concentration indication and other devices. The technology commonly used for sterilization in microbial incubators is UV lamp irradiation. For example, after opening the incubator, turn on the UV lamp in the room for irradiation, or install a UV lamp device inside the incubator for irradiation. Usually, the overall environment in an incubator cannot be separated and can only be used to cultivate microorganisms that grow and reproduce in a specific environment.

然而现有技术中存在以下缺陷:紫外等照射杀菌范围广而迅速,处理时间短。但孢子、孢囊和病毒等微生物对于紫外线照射的耐受性较高,不易被杀灭。但紫外照射杀菌没有持续消毒能力,可能存在微生物的光复活问题且不易做到在整个处理空间内辐射均匀,容易影响微生物的培养。并且一台培养箱一般只能用于培养在一种特定环境下生长繁殖的微生物,如果要培养多种需求不同培养环境的微生物,则需要联合使用多台微生物培养箱。此外,培养箱内温度、CO2浓度等指标无法自动记录,不利于实验室的连续质控及对于微生物培养环境的把控。另外,操作者只有在打开培养箱拿出培养物之后,才能观察到生物生长繁殖情况,并且目前常用的培养箱的门过大,频繁地开关容易造成污染。However, the prior art has the following defects: the sterilization range of ultraviolet and other irradiation is wide and rapid, and the treatment time is short. However, microorganisms such as spores, cysts and viruses are highly resistant to ultraviolet radiation and are not easily killed. However, ultraviolet irradiation sterilization has no continuous disinfection ability, there may be a problem of photoreactivation of microorganisms, and it is not easy to achieve uniform radiation in the entire processing space, which easily affects the cultivation of microorganisms. And an incubator can generally only be used to cultivate microorganisms that grow and reproduce in a specific environment. If you want to cultivate a variety of microorganisms that require different cultivation environments, you need to use multiple microbial incubators in combination. In addition, indicators such as temperature and CO2 concentration in the incubator cannot be automatically recorded, which is not conducive to the continuous quality control of the laboratory and the control of the microbial culture environment. In addition, the operator can only observe the growth and reproduction of the organisms after opening the incubator to take out the culture, and the door of the currently commonly used incubator is too large, and frequent opening and closing may easily cause pollution.

实用新型内容Utility model content

本实用新型的目的是克服现有技术中存在的不足,提供一种培养箱,解决了现有技术微生物培养箱通用性不强,且易发生交叉污染的问题。The purpose of the utility model is to overcome the deficiencies in the prior art and provide an incubator, which solves the problems that the prior art microbial incubator is not versatile and prone to cross-contamination.

为了实现上述目的,本实用新型采用的技术方案为:In order to achieve the above purpose, the technical scheme adopted by the present utility model is:

一种培养箱,包括箱体和外箱门,其特征在于,所述外箱门的一侧与箱体通过铰接的方式活动连接,所述箱体内设有多个隔热板层,每个隔热板层将箱体划分成多个隔间,每个隔间上还配备有内箱门,所述箱体上还安装有开关,每个所述隔间上还配备有管道系统,所述管道系统包括输送消毒气体管道、抽出培养箱内气体管道、输送二氧化碳管道、输送氧气管道和气体流通控制装置,每个所述隔间内还安装有紫外线灭菌灯、摄像头和监测设备,所述开关、管道系统、紫外线灭菌灯、摄像头和监测设备分别与控制器电性连接。An incubator includes a box body and an outer box door, characterized in that one side of the outer box door is movably connected to the box body by means of hinges, and the box body is provided with a plurality of heat insulation board layers, each of which is The box body is divided into a plurality of compartments by a heat insulation board layer, each compartment is also equipped with an inner box door, a switch is also installed on the box body, and each compartment is also equipped with a piping system, The pipeline system includes a pipeline for conveying sterilized gas, a pipeline for extracting gas from the incubator, a pipeline for conveying carbon dioxide, a pipeline for conveying oxygen, and a gas circulation control device, and each compartment is also equipped with an ultraviolet sterilization lamp, a camera and monitoring equipment, The switch, the pipeline system, the ultraviolet sterilization lamp, the camera and the monitoring equipment are respectively electrically connected with the controller.

优选的,所述隔间设有6个。Preferably, there are 6 compartments.

优选的,每个所述内箱门上安装有拉手环。Preferably, a handle ring is installed on each of the inner box doors.

优选的,所述紫外线灭菌灯和摄像头安装于隔间内的顶部,所述监测设备安装于隔间内的侧壁上。Preferably, the ultraviolet sterilization lamp and the camera are installed on the top of the compartment, and the monitoring device is installed on the side wall of the compartment.

优选的,所述输送消毒气体管道、抽出培养箱内气体管道、输送二氧化碳管道和输送氧气管道的管道内分别装配有气体滤网。Preferably, a gas filter screen is installed in the pipeline for conveying sterile gas, the pipeline for extracting gas in the incubator, the pipeline for conveying carbon dioxide and the pipeline for conveying oxygen.

优选的,每个所述隔间内安装有置物板。Preferably, a storage board is installed in each of the compartments.

本实用新型具备有益效果:本实用新型通过设计多个隔间可用于培养需求不同培养环境的微生物,可同时培养孵育需氧菌、厌氧菌、微需氧菌、真菌等微生物,且能够保证各种不同的微生物在固定的隔间里进行培养,不容易发生交叉污染的情况。以气体熏蒸和紫外照射两种消毒灭菌技术来保证后续微生物培养不会被污染。在管道系统中加装气体滤网既可过滤绝大部分微生物,而又不会影响到气体的进入和流通。通过安装摄像头和监测设备可保证操作者可以在不打开培养箱的情况下观察培养箱内微生物生长繁殖情况,减少污染的可能性。监测装置便于实验室进行长期连续监测和质量控制,为微生物培养孵育提供良好环境。The utility model has beneficial effects: the utility model can be used for culturing microorganisms requiring different culture environments by designing a plurality of compartments, and can simultaneously cultivate and incubate microorganisms such as aerobic bacteria, anaerobic bacteria, micro-aerobic bacteria, fungi, etc., and can ensure A variety of different microorganisms are cultivated in fixed compartments, and cross-contamination is not easy to occur. Two disinfection and sterilization technologies, gas fumigation and ultraviolet irradiation, are used to ensure that the subsequent microbial culture will not be contaminated. Adding a gas filter to the pipeline system can filter most microorganisms without affecting the entry and circulation of gas. By installing cameras and monitoring equipment, the operator can observe the growth and reproduction of microorganisms in the incubator without opening the incubator, reducing the possibility of contamination. The monitoring device facilitates long-term continuous monitoring and quality control in the laboratory, and provides a good environment for microbial culture and incubation.

附图说明Description of drawings

图1为本实用新型结构示意图;Fig. 1 is the structural representation of the utility model;

图2为本实用新型隔间结构示意图;Fig. 2 is the utility model compartment structure schematic diagram;

图3为图2的侧视图;Fig. 3 is the side view of Fig. 2;

图4为本实用新型箱体内部结构示意图;Figure 4 is a schematic diagram of the internal structure of the box body of the present invention;

图5为本实用新型气体滤网结构示意图;5 is a schematic structural diagram of a gas filter screen of the present invention;

图中:1、箱体,2、外箱门,3、隔热板层,4、隔间,5、内箱门,6、拉手环,7、开关,8、管道系统,81、输送消毒气体管道,82、抽出培养箱内气体管道,83、输送二氧化碳管道,84、输送氧气管道,85、气体流通控制装置,9、紫外线灭菌灯,10、摄像头,11、监测设备,12、气体滤网,13、置物板。In the picture: 1. Box body, 2. Outer box door, 3. Insulation board layer, 4. Compartment, 5. Inner box door, 6. Handle ring, 7. Switch, 8. Piping system, 81. Transport disinfection Gas pipeline, 82. Gas pipeline for drawing out the incubator, 83. Carbon dioxide pipeline, 84. Oxygen pipeline, 85. Gas circulation control device, 9. Ultraviolet sterilization lamp, 10. Camera, 11. Monitoring equipment, 12. Gas Filter, 13, storage board.

具体实施方式Detailed ways

下面结合附图对本实用新型作进一步详细说明。The present utility model will be described in further detail below in conjunction with the accompanying drawings.

如图1-5所示的一种培养箱,包括箱体1和外箱门2,其特征在于,所述外箱门2的一侧与箱体1通过铰接的方式活动连接,所述箱体1内设有多个隔热板层3,每个隔热板层3将箱体1划分成多个隔间4,每个隔间4上还配备有内箱门5,所述箱体1上还安装有开关7,每个所述隔间4上还配备有管道系统8,所述管道系统8包括输送消毒气体管道81、抽出培养箱内气体管道82、输送二氧化碳管道83、输送氧气管道84和气体流通控制装置85,每个所述隔间4内还安装有紫外线灭菌灯9、摄像头10和监测设备11,所述开关7、管道系统8、紫外线灭菌灯9、摄像头10和监测设备11分别与控制器电性连接。An incubator as shown in Figures 1-5 includes a box body 1 and an outer box door 2. It is characterized in that one side of the outer box door 2 is movably connected to the box body 1 by means of hinges. The body 1 is provided with a plurality of insulation board layers 3, each insulation board layer 3 divides the box body 1 into a plurality of compartments 4, and each compartment 4 is also equipped with an inner box door 5. The box body A switch 7 is also installed on the 1, and a piping system 8 is also provided on each of the compartments 4. The piping system 8 includes a pipeline 81 for conveying sterile gas, a pipeline 82 for extracting gas in the incubator, a pipeline 83 for conveying carbon dioxide, and a pipeline for conveying oxygen. The pipeline 84 and the gas circulation control device 85, each of the compartments 4 is also equipped with an ultraviolet sterilization lamp 9, a camera 10 and a monitoring device 11, the switch 7, the pipeline system 8, the ultraviolet sterilization lamp 9, the camera 10 and the monitoring device 11 are respectively electrically connected with the controller.

较佳地,所述隔间4设有6个。每个所述内箱门5上安装有拉手环6。所述紫外线灭菌灯9和摄像头10安装于隔间4内的顶部,所述监测设备11安装于隔间4内的侧壁上。所述输送消毒气体管道81、抽出培养箱内气体管道82、输送二氧化碳管道83和输送氧气管道84的管道内分别装配有气体滤网12。每个所述隔间4内安装有置物板13。Preferably, there are six compartments 4 . A handle ring 6 is installed on each of the inner box doors 5 . The ultraviolet sterilization lamp 9 and the camera 10 are installed on the top of the compartment 4 , and the monitoring device 11 is installed on the side wall of the compartment 4 . Gas filter screens 12 are installed in the pipelines for conveying sterilizing gas 81 , extracting gas in the incubator 82 , conveying carbon dioxide 83 and conveying oxygen 84 , respectively. Each of the compartments 4 is provided with a storage board 13 .

本实用新型是这样来实现的,培养箱包括箱体1和外箱门2,外箱门2位于箱体1前端(如图1所示),箱体1的侧面与外箱门2活动密封连接。培养箱内环境用隔热板层3分隔成6个独立的隔间4,隔热板层3可以使用双层真空隔热玻璃,但不仅限于使用这种材料,可以将双层真空隔热玻璃换成其他隔热、可形成密闭空间又不影响微生物培养的材质。内箱门5一侧通过与箱体1活动连接,每个独立区域的隔间4上的内箱门5均可以通过拉手环6进行开关,不影响其它独立区。每个区域可用于培养不同的微生物,明确固定分区。The utility model is realized in this way, the incubator includes a box body 1 and an outer box door 2, the outer box door 2 is located at the front end of the box body 1 (as shown in Figure 1), and the side surface of the box body 1 is movably sealed with the outer box door 2 connect. The environment in the incubator is divided into 6 independent compartments 4 by the thermal insulation board layer 3. The thermal insulation board layer 3 can use double-layer vacuum insulation glass, but it is not limited to the use of this material. Replace it with other materials that are insulated and can form a closed space without affecting the cultivation of microorganisms. One side of the inner box door 5 is movably connected to the box body 1, and the inner box door 5 on the compartment 4 of each independent area can be opened and closed through the handle ring 6, without affecting other independent areas. Each area can be used to cultivate different microorganisms, with clear fixed partitions.

每个隔间4中都装备有紫外线灭菌灯9、摄像头10以及培养箱内各项指标的监测设备11。监测设备11可监测每个隔间4内的温度、二氧化碳浓度、氧气浓度、气体压力等指标。另外,每个隔间4内都装备有用以放置培养皿或其它物件的置物板13,并且置物板13与隔间4底层的隔热板层3可以保持有一定距离,距离高低可根据具体实际情况进行调整,也可以与隔间4底层的隔热板层3不保持距离。Each compartment 4 is equipped with an ultraviolet sterilization lamp 9, a camera 10 and monitoring equipment 11 for various indicators in the incubator. The monitoring device 11 can monitor the temperature, carbon dioxide concentration, oxygen concentration, gas pressure and other indicators in each compartment 4 . In addition, each compartment 4 is equipped with a storage board 13 for placing petri dishes or other objects, and the storage board 13 and the heat insulation board layer 3 at the bottom of the compartment 4 can be kept at a certain distance, and the distance can be based on the actual situation. The situation can be adjusted, and the distance from the heat insulating board layer 3 on the bottom layer of the compartment 4 may not be kept.

每个独立的隔间4都装配有连接密闭的消毒气体甲醇-高锰酸钾混合气体或福尔马林气体的输送消毒气体管道81、抽出培养箱内气体管道82、输送二氧化碳管道83、输送氧气管道84和气体流通控制装置85。并且,在每一根管道靠近培养箱的一侧都装有气体滤网12,气体流通控制装置85分别用以过滤进入培养箱内部的气体和控制气体流通。输送消毒气体管道81、抽出培养箱内气体管道82、输送二氧化碳管道83和输送氧气管道84的粗细可根据具体实际情况进行调整,每根管道的粗细可以保持一致,也可以不完全相同。气体如二氧化碳需从二氧化碳罐中出来经过管道进入培养箱,则从二氧化碳罐到培养箱由同一根管道进行二氧化碳的运输。氧气以及其他气体的运输管道以及抽气管道的设计与二氧化碳运输管道类似。每根管道都会通过气体流通控制装置85来控制气体流动并根据监测设备11来控制气体某种成分的含量浓度。所以,此设计方便实验室同时在一个培养箱中孵育培养多种需要不同孵育环境的微生物,可同时培养孵育需氧菌、厌氧菌、微需氧菌、真菌等微生物,且能够保证各种不同的微生物在固定的隔间4里进行培养,不容易发生交叉污染的情况。并且,每次拿放培养基都可以只打开一个隔间4的内箱门5,有效预防污染的发生和扩散。Each independent compartment 4 is equipped with a sterile gas pipeline 81 , a gas pipeline 82 for extracting the gas in the incubator, a carbon dioxide delivery pipeline 83 , a gas pipeline 82 for extracting the gas in the incubator, and a pipeline 83 for transporting carbon dioxide. Oxygen conduit 84 and gas flow control device 85 . In addition, a gas filter 12 is installed on the side of each pipeline close to the incubator, and the gas flow control device 85 is used to filter the gas entering the incubator and control the gas flow. The thickness of the pipeline 81 for conveying the sterilizing gas, the pipeline 82 for extracting the gas in the incubator, the pipeline 83 for conveying carbon dioxide and the pipeline 84 for conveying oxygen can be adjusted according to the actual situation, and the thickness of each pipeline can be the same or not. The gas such as carbon dioxide needs to come out of the carbon dioxide tank and enter the incubator through the pipeline, then the carbon dioxide is transported from the carbon dioxide tank to the incubator by the same pipeline. The design of the oxygen and other gas transport pipelines and the extraction pipelines is similar to that of the carbon dioxide transport pipelines. Each pipeline will control the gas flow through the gas flow control device 85 and control the content concentration of a certain component of the gas according to the monitoring device 11 . Therefore, this design is convenient for the laboratory to incubate and cultivate a variety of microorganisms that require different incubation environments in one incubator at the same time. Different microorganisms are cultivated in the fixed compartment 4, and cross-contamination is not easy to occur. Moreover, the inner box door 5 of only one compartment 4 can be opened each time the medium is taken and placed, which can effectively prevent the occurrence and spread of contamination.

在第一次使用培养箱以及长时间不使用后又开始使用时,必须对培养箱进行消毒灭菌。本设计以独立的输送消毒气体管道81连接密闭的消毒气体甲醇-高锰酸钾混合气体或福尔马林气体,打开气体流通控制装置85让消毒气体进入培养箱内并充满整个空间。当气体熏蒸足够时间后,以抽出培养箱内气体管道82连接抽风装置(未图示)将培养箱内气体清空,以保证不会对后续微生物培养造成影响。之后可通过开关7打开紫外线灭菌灯9进行照射。以熏蒸和紫外照射两种消毒灭菌技术来保证后续微生物培养不会被污染。The incubator must be sterilized when it is used for the first time and when it is not used for a long time. In this design, an independent sterilizing gas pipeline 81 is used to connect the closed sterilizing gas methanol-potassium permanganate mixed gas or formalin gas, and the gas circulation control device 85 is opened to let the sterilizing gas enter the incubator and fill the entire space. After the gas has been fumigated for a sufficient time, the gas pipe 82 in the incubator is drawn out and connected to an air extraction device (not shown) to empty the gas in the incubator, so as to ensure that the subsequent microbial culture will not be affected. Afterwards, the ultraviolet sterilization lamp 9 can be turned on by the switch 7 for irradiation. Two disinfection and sterilization technologies, fumigation and ultraviolet irradiation, are used to ensure that subsequent microbial cultures will not be contaminated.

在隔间4内装备有摄像头10和监测设备11,摄像头10和监测设备11分别与控制器(未图示)电性连接,通过控制器显示检测到的各项指标。使得操作者可以在不打开培养箱的情况下观察培养箱内微生物生长繁殖情况,减少污染的可能性。置物板13的材质可以是铝制或不锈钢制。The compartment 4 is equipped with a camera 10 and a monitoring device 11. The camera 10 and the monitoring device 11 are respectively electrically connected to a controller (not shown), and the detected indicators are displayed through the controller. The operator can observe the growth and reproduction of microorganisms in the incubator without opening the incubator, reducing the possibility of contamination. The material of the storage board 13 may be aluminum or stainless steel.

上述实施方式是对本实用新型的说明,不是对本实用新型的限定,可以理解在不脱离本实用新型的原理和精神的情况下可以对这些实施例进行多种变化、修改、替换和变型,本实用新型的保护范围由所附权利要求及其等同物限定。The above embodiment is an illustration of the present invention, not a limitation of the present invention. It can be understood that these embodiments can be changed, modified, replaced and modified without departing from the principle and spirit of the present The scope of protection of the new type is defined by the appended claims and their equivalents.

Claims (6)

1. The utility model provides an incubator, includes box (1) and outer case door (2), its characterized in that, one side and box (1) of outer chamber door (2) are through articulated mode swing joint, be equipped with a plurality of heat insulating board layer (3) in box (1), every heat insulating board layer (3) divide into a plurality of compartments (4) with box (1), still be equipped with interior chamber door (5) on every compartment (4), still install switch (7) on box (1), every still be equipped with pipe-line system (8) on compartment (4), pipe-line system (8) are including carrying disinfection gas pipeline (81), taking out incubator interior gas pipeline (82), carrying carbon dioxide pipeline (83), carrying oxygen pipeline (84) and gaseous circulation controlling means (85), every still install ultraviolet sterilamp (9) in compartment (4), Camera (10) and monitoring facilities (11), switch (7), pipe-line system (8), ultraviolet sterilamp (9), camera (10) and monitoring facilities (11) respectively with controller electric connection.
2. An incubator as claimed in claim 1, characterised in that there are 6 compartments (4).
3. An incubator according to claim 1, characterised in that each inner door (5) is fitted with a bracelet (6).
4. An incubator as claimed in claim 1, characterised in that said UV germicidal lamp (9) and said camera (10) are mounted on the top inside the compartment (4) and said monitoring device (11) is mounted on the side wall inside the compartment (4).
5. An incubator according to claim 1, characterised in that the ducts for the transport of sterile gas (81), for the evacuation of gases (82) in the incubator, for the transport of carbon dioxide (83) and for the transport of oxygen (84) are equipped with respective gas screens (12).
6. An incubator as claimed in claim 1, characterised in that a shelf (13) is mounted in each compartment (4).
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113797368A (en) * 2021-11-03 2021-12-17 上海搏旅仪器有限公司 Two-in-one efficient sterilization incubator

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113797368A (en) * 2021-11-03 2021-12-17 上海搏旅仪器有限公司 Two-in-one efficient sterilization incubator

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