CN203960207U - A kind of container for biological detection - Google Patents
A kind of container for biological detection Download PDFInfo
- Publication number
- CN203960207U CN203960207U CN201420366083.1U CN201420366083U CN203960207U CN 203960207 U CN203960207 U CN 203960207U CN 201420366083 U CN201420366083 U CN 201420366083U CN 203960207 U CN203960207 U CN 203960207U
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- container
- tube
- nucleic acid
- tube body
- detection
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
一种用于生物检测的容器，其包括一个容器主体，一个入口通道，一个出口通道；该容器主体包括一个管体和一个与管体相连的管盖，管体的上部是圆筒形的，下部是锥形的；管体的两侧各设有一个开口；在管体内部设置有两个通道，连接至管体两侧的开口，形成一个入口通道，一个出口通道。入口通道连接至管体圆筒形上部的三分之二处，出口通道连接至管体的锥形底部。本实用新型不仅可以实现整个的核酸检测实验，也可进行单一的核酸提取或PCR扩增实验，用途广泛。本实用新型使实验过程仅对单管容器操作，控制简单，容易实现自动化。
A container for biological detection, which includes a container body, an inlet channel, and an outlet channel; the container body includes a tube body and a tube cover connected to the tube body, the upper part of the tube body is cylindrical, The lower part is tapered; two openings are provided on both sides of the pipe body; two passages are arranged inside the pipe body, connected to the openings on both sides of the pipe body, forming an inlet passage and an outlet passage. The inlet channel is connected to the cylindrical upper two thirds of the body and the outlet channel is connected to the conical bottom of the body. The utility model can not only realize the whole nucleic acid detection experiment, but also perform single nucleic acid extraction or PCR amplification experiment, and has wide applications. The utility model makes the experimental process only operate on a single-tube container, has simple control, and is easy to realize automation.
Description
Technical field
The present invention relates to a kind of container for biological detection, belong to bio-science and nanometer material science field.
Background technology
Detection of nucleic acids is the general name of a series of technology of direct-detection pathogen nucleic acid.Its basic step comprises nucleic acid extraction, amplification and detects.In laboratory, each step of conventional detection of nucleic acids is to carry out respectively in different instruments or container: nucleic acid extraction is in instrument for extracting nucleic acid, and amplification, in pcr amplification instrument, detects and passes through agarose gel electrophoresis.So, in experimentation, there will be unavoidably the pollution of sample reagent, affect the accuracy of laboratory test results.So in order to address the above problem, the present invention designs a kind of container for detection of biological samples, not only can realize whole detection of nucleic acids experiment, also can carry out single nucleic acid extraction or pcr amplification experiment, of many uses.Also can make experimentation only to single tube container operation, control simply, easily realize automatization.
Summary of the invention
The object of the present invention is to provide a kind of container for biological detection, in this container, can carry out the testing process of whole sample of nucleic acid, without agent transfer operation, avoided the pollution of reagent.
For realizing above-mentioned functions, the present invention takes following technical scheme: a kind of container for biological detection, and it comprises a container body, an access road, an exit passageway; This container body comprises the pipe lid that a body is connected with body with, and the top of body is columnar, and bottom is taper; The both sides of body are respectively provided with an opening; In body inside, be provided with two passages, be connected to the opening of body both sides, form an access road, an exit passageway.
The further restriction of technique scheme is: this body is to be made by the transparent material of aseptic deactivation.
The further restriction of technique scheme is: access road extends to 2/3rds places of body cylinder-shaped upper part inwall, and exit passageway extends to the conical lower portion of body inside.
The further restriction of technique scheme is: access road and exit passageway can be the plastics tubings of inserting in body, can be to be also embedded in the passage surrounding with body in body, is not particularly limited.
In detection of nucleic acids experiment, sample first joins in body with liquid-transfering gun by opening pipe lid, and the required reagent of ensuing nucleic acid extraction and pcr amplification can add by access road successively, and the waste liquid producing in process is discharged by exit passageway.Compared with prior art, the present invention not only can realize whole detection of nucleic acids experiment, also can carry out single nucleic acid extraction or pcr amplification experiment, of many uses.The present invention makes experimentation only to single tube container operation, controls simply, easily realizes automatization.
Accompanying drawing explanation:
Figure of description is overall appearance schematic diagram of the present invention
In figure: container (10), pipe lid (11), body (12), body top (13), and body bottom (14), entrance (15), access road (16), outlet (17), exit passageway (18),
Embodiment:
Below in conjunction with accompanying drawing, preferred embodiment of the present invention is elaborated, so that clearer understanding object of the present invention, characteristics and advantages.It should be understood that embodiment shown in the drawings is not limitation of the scope of the invention, and just for the connotation of technical solution of the present invention is described.
As shown in Figure of description, the 10th, a kind of container for biological detection.11 is the pipe lid of container.Before detection of nucleic acids starts, with liquid-transfering gun, sample is joined in container 10 to lid upper tube cap 11.First step of detection of nucleic acids is nucleic acid extraction, and what in the implementation case, adopt is that paramagnetic particle method is adjusted extraction.The mixed solution of magnetic bead and lysate is entered in body 12 along access road 16 from the entrance 15 on body top 13 by outside injection device, after sufficient cracking, a magnet is pressed close to body bottom 14, and magnetic bead reunion is adsorbed on body bottom 14 in a moment.Now, utilize outside liquid sucking device by waste liquid along exit passageway 18 from exporting 17 absorptions out.
Repeat said process, then pass into successively different scavenging solutions, finally from entrance 15 and access road 16, add elutriant to carry out, wash-out, completing nucleic acid extraction step.From entrance 15, add the required reagent of PCR again, sealing outlet 17 and entrance 15, put into real-time fluorescence PCR instrument by container and carry out real-time fluorescence PCR amplification, when having increased, detects and also complete.
The present invention makes experimentation only to single tube container operation, controls simply, easily realizes automatization.
Claims (1)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420366083.1U CN203960207U (en) | 2014-07-04 | 2014-07-04 | A kind of container for biological detection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420366083.1U CN203960207U (en) | 2014-07-04 | 2014-07-04 | A kind of container for biological detection |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203960207U true CN203960207U (en) | 2014-11-26 |
Family
ID=51920816
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420366083.1U Expired - Fee Related CN203960207U (en) | 2014-07-04 | 2014-07-04 | A kind of container for biological detection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203960207U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106399053A (en) * | 2016-11-14 | 2017-02-15 | 湖南圣湘生物科技有限公司 | PCR reaction device and using method thereof |
| WO2017201046A1 (en) * | 2016-05-17 | 2017-11-23 | Integrated Nano-Technologies, Inc. | Filtration column assembly for diagnostic assay system |
-
2014
- 2014-07-04 CN CN201420366083.1U patent/CN203960207U/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2017201046A1 (en) * | 2016-05-17 | 2017-11-23 | Integrated Nano-Technologies, Inc. | Filtration column assembly for diagnostic assay system |
| US11148135B2 (en) | 2016-05-17 | 2021-10-19 | Integrated Nano-Technologies, Inc. | Filtration column assembly for diagnostic assay system |
| CN106399053A (en) * | 2016-11-14 | 2017-02-15 | 湖南圣湘生物科技有限公司 | PCR reaction device and using method thereof |
| CN106399053B (en) * | 2016-11-14 | 2019-07-05 | 湖南圣湘生物科技有限公司 | A kind of PCR reaction unit and its application method |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141126 Termination date: 20150704 |
|
| EXPY | Termination of patent right or utility model |