CN202157065U - Continuous culture device for plankton - Google Patents
Continuous culture device for plankton Download PDFInfo
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- CN202157065U CN202157065U CN201120291992.XU CN201120291992U CN202157065U CN 202157065 U CN202157065 U CN 202157065U CN 201120291992 U CN201120291992 U CN 201120291992U CN 202157065 U CN202157065 U CN 202157065U
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- glass tube
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- planktonic organism
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/02—Photobioreactors
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/34—Internal compartments or partitions
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
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Abstract
本实用新型提供了一种浮游生物连续培养装置，包括底座、安装于所述底座上的外玻璃管、位于所述外玻璃管内侧的内玻璃管及位于所述外玻璃管内侧的隔板，所述内玻璃管与所述外玻璃管之间设有一环形收容腔，所述隔板位于该收容腔内并将该收容腔分隔成一进水室及一出水室，所述外玻璃管上设有连通所述进水室的进水口及连通所述出水室的出水口，所述进水口高于所述出水口。本实用新型所述的浮游生物连续培养装置，满足营养盐浓度恒定的要求，也增加营养液中的溶解氧，有助于浮游生物的生长。
The utility model provides a continuous culture device for plankton, comprising a base, an outer glass tube installed on the base, an inner glass tube located inside the outer glass tube and a partition located inside the outer glass tube, An annular storage cavity is provided between the inner glass tube and the outer glass tube, the partition is located in the storage cavity and divides the storage cavity into a water inlet chamber and a water outlet chamber, and the outer glass tube is provided with There is a water inlet connected to the water inlet chamber and a water outlet connected to the water outlet chamber, and the water inlet is higher than the water outlet. The plankton continuous culture device described in the utility model meets the requirement of constant nutrient salt concentration, and also increases the dissolved oxygen in the nutrient solution, which is helpful for the growth of plankton.
Description
[technical field]
The utility model relates to a kind of planktonic bactogen, relates in particular to a kind of cultured continuously to mikrobes such as planktonic algae, planktobacterias.
[background technology]
The training method of planktonic organism in the laboratory comprises cultured continuously, semicontinuous cultivation and restricted cultivation.Wherein semicontinuous cultivation is to the no particular requirement of device, as long as satisfy the experiment demand, regular gets final product according to certain ratio replacing nutritive medium; Cultured continuously then requires nutrient solution concentration constant.In culture of continuous cultivation, accomplish that nutrient concentration is constant, be very important for accuracy of experimental results.Solving the main way of this difficult problem at present is exactly through calculating planktonic growth rate; Quantity according to the nutritive salt of planktonic organism consumption in the growth rate pro forma invoice bit time; Then according to the estimation result; By artificial or have computer software control regularly to add nutritive medium, the nutritive medium that consumes with compensation.But such training method is exactly discontinuous cultivation in essence, and only with lower nutritive medium turnover rate, more supply number of times satisfies the nutrition constant as much as possible and cultivates requirement.But this training method is difficult to the consumption of the interior nutritive salt of pro forma invoice bit time accurately, and its reason mainly is: (1) in this training method process, not only original planktonic organism is consuming nutritive medium, and the planktonic organism of added value is also consuming nutritive medium; (2) planktonic organism is in different growth phases, and vital activity and metabolism are also different, and the nutritive salt speed of consumption also is not quite similar.
[utility model content]
The utility model technical problem to be solved is to provide a kind of planktonic organism bactogen, and it can effectively keep the constant of nutritive medium in the growth room, and need not artificial stirring can increase dissolved oxygen.
For solving the problems of the technologies described above; The utility model adopts following technical scheme: a kind of planktonic organism bactogen; Comprise base, be installed on outer glass pipe on the said base, be positioned at the inboard inner glass tube of said outer glass pipe and be positioned at the inboard dividing plate of said outer glass pipe; Be provided with an annular host cavity between said inner glass tube and the said outer glass pipe; Said dividing plate is positioned at this host cavity and this host cavity is separated into an intake chamber and a water-supplying chamber, and said outer glass pipe is provided with water-in that is communicated with said intake chamber and the water outlet that is communicated with said water-supplying chamber, and said water-in is higher than said water outlet.
Further, be formed with a planktonic growth room in the said inner glass tube, and this inner glass tube is provided with feed pathway, liquid outlet channel and the thief hole that is communicated with said growth room, said thief hole extends on the said outer glass pipe from said inner glass tube.
Further, said feed pathway is lower than said liquid outlet channel.
Further, said outer glass pipe is provided with the spillway that is communicated with said intake chamber, and this spillway is higher than said water-in.
Further, the bottom of said outer glass pipe is provided with two water port that are communicated with said intake chamber and water-supplying chamber respectively.
Further, be equipped with a valve on said feed pathway, liquid outlet channel and the thief hole, and also further be provided with a membrane module on the feed pathway, liquid outlet channel.
Further, said membrane module comprise a cylinder-shaped body, be positioned at bottom part body netted bottom surface, be positioned at the opening at main body top and be positioned at said body interior and according to the sealing-ring, netted cover plate and the filter membrane that set gradually away from said opening direction.
Further, said intake chamber and said water-supplying chamber are two spaces that independence does not communicate.
The described planktonic organism bactogen of the utility model, the constant requirement that it had both satisfied nutrient concentration can replace hand mixing again, and the dissolved oxygen of the liquid that has additional nutrients helps planktonic growth.In the passage of device planktonic organism culturing room, the filter membrane that the aperture varies in size is installed, both can have been satisfied the planktonic cultivation requirement that varies in size, the inner planktonic organism of assurance device is not outflowed again.
[description of drawings]
Fig. 1 is the structural representation of the described a kind of planktonic organism bactogen of the utility model.
Fig. 2 is the synoptic diagram of the membrane module of the described a kind of planktonic organism bactogen of the utility model.
Wherein, 1, outer glass pipe; 2, inner glass tube; 3, dividing plate; 4, base; 5, water-in; 6, water outlet; 7, spillway; 8, thief hole; 9, feed pathway; 10, liquid outlet channel; Youngster, valve; 12, membrane module; 121, main body; 122, netted bottom surface; 123, opening; 13, netted cover plate; 14, filter membrane; 15, sealing-ring; 16, intake chamber; 17, water-supplying chamber; 18, growth room; 19, water port.
[embodiment]
Like Fig. 1 and shown in Figure 2; The utility model provides a kind of planktonic organism bactogen 100, comprise cup dolly 4, be installed on the circle on the said base outer glass pipe 1, be positioned at the inboard inner glass tube 2 of said outer glass pipe 1 and be positioned at the inboard dividing plate 3 of said outer glass pipe.
Said inside and outside Glass tubing all is annular; Its bottom is fixed on the said base 4; And said inner glass tube 2 is the socket of concentric(al) circles mode with said outer glass pipe 1; Form an annular host cavity at inner glass tube 2 and 1 of outer glass pipe thus, supply the growth room 18 of planktonic organism growth and form one in the said inner glass tube 2.
Said dividing plate 3 is positioned at said annular host cavity, and said annular host cavity is cut off into two independent parts, be respectively intake chamber 16 and water-supplying chamber 17, and said intake chamber 16 is two spaces that do not communicate with said water-supplying chamber 17.
The top of said outer glass pipe 1 is provided with the water-in 5 that is communicated with said intake chamber 16, is higher than said water-in 5 and is communicated with the spillway 7 of intake chamber 16 and is positioned at said water-supplying chamber 17 1 sides and is communicated with the water outlet 6 of said water-supplying chamber 17, and the below of said outer glass pipe 1 is provided with two water port 19 that lay respectively at said intake chamber 16 1 sides and water-supplying chamber's 17 1 sides.Said inner glass tube 2
The thief hole 8 that said inner glass tube 2 is provided with the feed pathway 9 that is communicated with said intake chamber 16, the liquid outlet channel 10 that is communicated with said water-supplying chamber 17 and extends and pass through said outer glass pipe 1; Said feed pathway 9 is circle with liquid outlet channel 10, and it is mainly used in and supplies nutritive medium to feed in the growth room 18 in the said inner glass tube 2.Said thief hole 8 is lower than the water outlet 6 on the said outer glass pipe 1, and it is mainly used in takes a sample to the liquid in the growth room 18.
In addition, be provided with a valve on water port 19 on the said outer glass pipe 1 and the thief hole 8, and water outlet 6 is starkly lower than said water-in 5.Be provided with valve 11 on liquid outlet channel 10 that is provided with on the said inner glass tube 2 and the feed pathway 9, and the material of said liquid outlet channel and feed pathway is synthetic glass.
Wherein, Be equipped with a membrane module 12 on said feed pathway 9 and the liquid outlet channel 10; This membrane module 12 is cylindrical; And in a spiral manner feed pathway 9 is carried out tight seal, and please cooperate and consult shown in Figure 2ly, said membrane module 12 comprises cylinder-shaped body 121, be positioned at the netted bottom surface 122 of bottom part body, be positioned at the opening 123 at main body top and be positioned at sealing-ring 15, netted cover plate 13 and the filter membranes 14 that said main body 121 is inner and from top to bottom set gradually.The diameter of said netted cover plate 13 is identical with the internal diameter of main body 121, and the area of each grid on the netted cover plate 13 is about 1cm2.Said rubber seal 15 is a ring texture, and the internal diameter size of external diameter and main body 121 is identical, and thickness satisfies seal request.The pore size of filter membrane 14 is according to planktonic size decision.
The utility model adopts the principle of communicating vessels and waterhead that the nutritive medium in the device is flowed.During use; Utilize micro pump that nutrient salt solution is promoted to intake chamber 16 from nutrient salt solution freight container (not shown),, therefore have waterhead between the two because water-in 5 is higher than water outlet 6; According to law of connected vessels; Nutritive medium gets into planktonic growth room 18 through the feed pathway 9 of inner glass tube 2, gets into water-supplying chamber 17 by liquid outlet channel 10 then, and last nutritive medium flows out through the water outlet 6 of outer glass pipe 1 naturally.When changing the filter membrane 14 in the membrane module 12; Want the valve open of elder generation with water port 19; The nutritive medium of intake chamber 16 and water-supplying chamber 17 is discharged, because feed pathway 9 is provided with membrane module 12 with liquid outlet channel 10, so the nutritive medium of planktonic organism growth room 18 the phenomenon that flows backwards fast can not occur; Close the valve 11 of feed pathway 9 and liquid outlet channel 10 then rapidly, membrane module 12 is taken off the back change filter membrane 14; After changing filter membrane 14, open the valve 11 of feed pathway 9 and liquid outlet channel 10, close water port 19, utilize micro pump that nutritive medium is promoted to intake chamber 16 once more.
The described planktonic organism bactogen of the utility model utilizes technique of backflow to prevent the nutritive medium waste; Nutritive medium is pumped into intake chamber 16 if micro pump is too much; For preventing that nutritive medium from overflowing; On the outer glass pipe 1 of device, a spillway 7 is set, too much nutritive medium can pass through spillway 7 backflow nutritive medium freight containers (not shown).
In sum, the described planktonic organism bactogen 100 of the utility model has the following advantages:
1), the nutrient concentration in this device keeps constant, need not manual work or instrument regularly adds, avoided because of the variation of nutrient concentration influence the planktonic organism growth.
2), nutrient salt solution mobile power is the waterhead that relies on water-in 5 and water outlet 6 in this device.Nutrient salt solution sluggish flow in this device increases the dissolved oxygen in the water body, helps planktonic growth, has avoided the manual regular stirring.
3), the filtering membranes 14 in the membrane module 12 can change according to the planktonic organism size of cultivating, the planktonic organism that suitable cultivation varies in size, and periodic replacement can prevent that also hydrobiont from and attach filter membrane 14 to cause water body to flow not smooth.
The above only is the most preferred embodiment of the utility model, is not the utility model is done any pro forma restriction, all belongs within the scope of the utility model technical scheme.Any those of ordinary skill in the art are not breaking away under the utility model technical scheme scope situation, utilize the method content of above-mentioned announcement that the utility model technical scheme is made many possible changes and modification, all belong to the scope of claims protection.
Claims (8)
1. planktonic organism bactogen; Comprise base, be installed on outer glass pipe on the said base, be positioned at the inboard inner glass tube of said outer glass pipe and be positioned at the inboard dividing plate of said outer glass pipe; It is characterized in that: be provided with an annular host cavity between said inner glass tube and the said outer glass pipe; Said dividing plate is positioned at this host cavity and this host cavity is separated into an intake chamber and a water-supplying chamber; Said outer glass pipe is provided with water-in that is communicated with said intake chamber and the water outlet that is communicated with said water-supplying chamber, and said water-in is higher than said water outlet.
2. planktonic organism bactogen according to claim 1; It is characterized in that: be formed with a planktonic growth room in the said inner glass tube; And this inner glass tube is provided with feed pathway, liquid outlet channel and the thief hole that is communicated with said growth room, and said thief hole extends on the said outer glass pipe from said inner glass tube.
3. planktonic organism bactogen according to claim 2 is characterized in that: said feed pathway is lower than said liquid outlet channel.
4. planktonic organism bactogen according to claim 3 is characterized in that: said outer glass pipe is provided with the spillway that is communicated with said intake chamber, and this spillway is higher than said water-in.
5. planktonic organism bactogen according to claim 4 is characterized in that: the bottom of said outer glass pipe is provided with two water port that are communicated with said intake chamber and water-supplying chamber respectively.
6. according to any described planktonic organism bactogen in the claim 2 to 5, it is characterized in that: be equipped with a valve on said feed pathway, liquid outlet channel and the thief hole, and also further be provided with a membrane module on the feed pathway, liquid outlet channel.
7. planktonic organism bactogen according to claim 6 is characterized in that: said membrane module comprises a cylinder-shaped body, is positioned at the netted bottom surface of bottom part body, is positioned at the opening at main body top and is positioned at said body interior and complies with sealing-ring, netted cover plate and the filter membrane that sets gradually away from said opening direction.
8. planktonic organism bactogen according to claim 6 is characterized in that: said intake chamber and said water-supplying chamber are two spaces that independence does not communicate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120291992.XU CN202157065U (en) | 2011-08-12 | 2011-08-12 | Continuous culture device for plankton |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201120291992.XU CN202157065U (en) | 2011-08-12 | 2011-08-12 | Continuous culture device for plankton |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202157065U true CN202157065U (en) | 2012-03-07 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201120291992.XU Expired - Fee Related CN202157065U (en) | 2011-08-12 | 2011-08-12 | Continuous culture device for plankton |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202157065U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105948264A (en) * | 2016-06-06 | 2016-09-21 | 浙江国科生态技术研究有限公司 | Bioreactor and eutrophic water body treating method thereof |
| CN110521644A (en) * | 2019-09-30 | 2019-12-03 | 中国水利水电科学研究院 | A kind of plankton separation incubator |
-
2011
- 2011-08-12 CN CN201120291992.XU patent/CN202157065U/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105948264A (en) * | 2016-06-06 | 2016-09-21 | 浙江国科生态技术研究有限公司 | Bioreactor and eutrophic water body treating method thereof |
| CN105948264B (en) * | 2016-06-06 | 2019-02-26 | 浙江国科生态技术研究有限公司 | A kind of bioreactor and its processing method to eutrophication water |
| CN110521644A (en) * | 2019-09-30 | 2019-12-03 | 中国水利水电科学研究院 | A kind of plankton separation incubator |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20120307 Termination date: 20120812 |