CN201622263U - Vertical electrophoresis system - Google Patents

Abstract

The utility model discloses a vertical electrophoresis system which comprises a tank body and a base positioned in the tank body, wherein one side surface of the base is provided with a gel chamber communicating the tank body with the base, the upper edge in the colloid chamber is clamped with a comb, and the wire electrodes arranged in the base and the tank body are respectively connected with a positive electrode and a negative electrode of a power supply. The gel chamber is combined by two gelatin plates with U-shaped cross section, the openings of the two gelatin plates are arranged oppositely, the inner side of the side wall of the fixed gel plate fixed on one side of the base and the outer side of the side wall of the other movable gel plate are matched in size and fastened with each other, when the movable gel plate is arranged on the fixed gel plate, the lower end surfaces of the two are in the same horizontal line, and the upper end surface of the fixed gel plate is lower than that of the movable gel plate. The vertical electrophoresis system has the characteristics of simple operation, high reliability, variable glue manufacturing areas, and the like.

Description

Vertical Electrophoresis System

technical field

The utility model relates to an experimental instrument for biochemical analysis, in particular to a vertical electrophoresis system.

Background technique

Electrophoresis is a common method for separating and identifying proteins in the teaching and research of biochemistry and molecular biology, and has been widely used in medical analysis, scientific research and drug development. The existing vertical electrophoresis tank, as shown in the Chinese utility model patent whose notification number is CN201108810Y, includes a tank body and a base located in the tank body. One side of the base is provided with a double-layer gel plate, and between the two gel plates A clamping strip is provided, and a comb is clamped on the upper edge between the two layers of gel plates, and the electrode wires provided in the base and the tank body are respectively connected with the positive pole and the negative pole of the power supply. The two separated gel plates are aligned with the two independent clamping strips and clamped with clips to seal the bottom to form a glue chamber. During the experiment, the liquid gel was poured into the glue chamber, and the comb was taken out after the gel was polymerized. Add the sample to be analyzed into the gel, and at the same time add the buffer into the buffer pool formed by the tank and the base, and electrophoresis can be performed after the power is turned on. Since the clamp strips and the gel plate are only connected by clips, it is difficult to fix the positional relationship between the two independent clamp strips and the separated gel plate, the operation is complicated, and the fixing effect is not ideal. The liquid gel is easy to flow from both sides of the glue chamber and/or or the bottom leaks out, so that the electrophoresis experiment cannot be carried out; in addition, the area of the gel plate produced each time cannot be changed, and when there are many experimental samples and more than one gel plate is needed, the operation of gel making will become more complicated and affect the production efficiency. The speed of the gel; and when the experimental sample is small and the area of the glue plate is small, the gel will be wasted.

Utility model content

The technical problem to be solved by the utility model is to provide a vertical electrophoresis system with simple operation and high reliability.

In order to solve the above problems, the vertical electrophoresis system designed by the utility model includes a tank body and a base located in the tank body. A glue chamber is provided on one side of the base, and the glue chamber is connected to the tank body and the base. Along the clamping comb, the electrode wires provided in the base and the tank are respectively connected to the positive and negative poles of the power supply. The difference is that the glue chamber is composed of two gel plates with a U-shaped section. The opening of the gel plate is set opposite to each other, and the inside of the side wall of the fixed gel plate fixed on one side of the base is fastened with the outer dimension of the side wall of the other movable gel plate. When the movable gel plate is installed on the fixed gel plate When going up, the lower end surfaces of the two are on the same horizontal line, and the height of the upper surface of the fixed gel plate is lower than the height of the upper surface of the movable gel plate.

In order to facilitate the installation of the fixed gel plate and the movable gel plate, the inner side of the side wall of the fixed gel plate and the outer side of the movable gel plate are provided with longitudinally extending guiding devices preferably in cooperation. Among them, the preferred guiding devices are guide rails and guide grooves.

The movable gel plate described in the above solution is preferably two or more. When the movable gel plate is installed on the fixed gel plate, the height of the upper surface of the movable gel plate increases gradually from the inside to the outside. In this way, when there are many experimental samples, two or more rubber sheets can be made at one time.

The vertical electrophoresis system described in the utility model preferably also includes one or more sheet-shaped insulating baffles, the thickness of the insulating baffles is consistent with the thickness of the glue chamber, and the width of the insulating baffles is smaller than the width of the glue chamber. The insulating baffle is inserted into the glue chamber before glue making, which is used to reduce the area of glue making, so as to prevent waste caused by small experimental samples.

In order to make the experimental electricity more even, the height of the insulating baffle described in the above scheme is consistent with the height of the fixed baffle.

Since there are generally one or more types of gels poured into the glue chamber, in order to allow the experimenter to intuitively understand the amount of gel poured and control its ratio, it is preferable to have a scale on the movable gel. In addition, it is also preferable to have a scale on the comb described in the above solution, and adjust the injection volume by controlling the insertion depth of the comb.

Compared with the prior art, the utility model is composed of two oppositely arranged U-shaped gel plates, the operation is simpler, and the relative shaking between the fixed gel plate and the movable gel plate can be effectively avoided , to prevent the leakage of glue; at the same time, by increasing the number of movable gel plates and insulating baffles, it is possible to easily change the area of the glue plate, improve the efficiency of glue production or reduce the waste of gel.

Description of drawings

Fig. 1 is a top view of a vertical electrophoresis system of a preferred embodiment of the present invention;

Fig. 2 is a longitudinal sectional view of Fig. 1 .

The labels in the figure are: 1. tank body; 2. base; 3. glue chamber; 3-1 fixed gel plate; 3-2 movable gel plate; 3-3. guiding device; 3-4. insulating baffle ; 4, comb; 5, electrode wire.

Detailed ways

A vertical electrophoresis system of a preferred embodiment of the present invention is shown in Figure 1 and Figure 2, comprising a tank body 1 and a base 2 located in the tank body 1, a side of the base 2 is vertically provided with a glue chamber 3, The glue chamber 3 is connected to the tank body 1 and the base 2, and the comb 4 is clamped on the upper edge between the glue chamber 3. The base 2 and the tank body 1 are respectively provided with electrode wires 5, and the electrode wire 5 located in the base 2 is connected to the power supply. The negative pole is connected, and the electrode wire 5 located in the tank body 1 is connected with the positive pole of the power supply. The above-mentioned glue chamber 3 is composed of two gel plates with a U-shaped cross-section. The openings of the two gel plates are arranged opposite to each other. The outer dimension of the side wall of another movable gel plate 3-2 is matched and fastened. When the movable gel plate 3-2 is installed on the fixed gel plate 3-1, the lower end surfaces of the two are on the same horizontal line, and the fixed gel plate The height of the upper end surface of the rubber plate 3-1 is lower than the height of the upper end surface of the movable gel plate 3-2. In order to facilitate the installation of the fixed gel plate 3-1 and the movable gel plate 3-2, the inside of the side wall of the fixed gel plate 3-1 cooperates with the outside of the side wall of the movable gel plate 3-2. A longitudinally extending guide 3-3. In a preferred embodiment of the present invention, the above-mentioned guiding device 3-3 is a guide rail and a guide groove. In addition, in order to allow the experimenter to understand the amount of gel in the gel chamber 3 more intuitively, to control the injection amount or the ratio of the gel, the movable gel plate 3-2 and/or the comb 4 are provided with scales .

Movable gel plate 3-2 is preferably two or more than two pieces, what cooperates with it is, is positioned at the side wall inner side of fixed gel plate 3-1 and the side wall outside of movable gel plate 3-2 The guiding device 3-3 is one or more than one. When the movable gel plate 3-2 is installed on the fixed gel plate 3-1, the lower end surfaces of all the movable gel plates 3-2 and the lower end surfaces of the fixed gel plate 3-1 are on the same horizontal plane, and each The height of the upper surface of the movable gel plate 3-2 gradually increases from the inside to the outside, so that the buffer in the matrix can communicate with each gel plate, and realize simultaneous electrophoresis of multiple gel plates. In this way, when there are many experimental samples, two or more rubber sheets can be made at one time. In addition, the vertical electrophoresis system preferably also includes one or more sheet-shaped insulating baffles 3-4, and the insulating baffles 3-4 are used to reduce the area of gel making to prevent waste caused by small experimental samples. . The thickness of the insulating baffle 3-4 should be consistent with the thickness of the glue chamber 3, and the width of the insulating baffle 3-4 should be less than the width of the glue chamber 3, and the height of the insulating baffle 3-4 is particularly required. The height of the insulating baffle 3-4 can be lower than the height of the fixed gel plate 3-1, and can be equal to or even higher than the height of the movable gel plate 3-2. More averagely, the height of the insulating baffle 3-4 described in the preferred embodiment of the present invention is consistent with the height of the fixed baffle.

Before the experiment, the base 2 was taken out from the tank body 1 and placed on a flat table. Select the specifications and number of corresponding movable gel plates 3-2 (the thickness of the glue chamber 3 is determined by the height of the side walls of the movable gel plates 3-2), and install them on the fixed gel plate via the guide device 3-3. Plate 3-1. When the installation is completed, let the movable gel plate 3-2 and the lower end surface of the fixed gel plate 3-1 be on the same horizontal line, and seal the bottom of the glue chamber 3 with transparent glue. Insert the comb 4 into the upper edge between the glue chambers 3, and pour liquid gel into the glue chamber 3, and take out the comb 4 after the gel polymerizes. Of course, before making glue, we can selectively increase the movable gel plate 3-2 or the insulating baffle 3-4 according to the amount of experimental samples, so as to control the amount of gel in the glue chamber 3 and change the area of the glue plate. After the glue production is completed, the transparent glue at the bottom of the glue chamber 3 is removed, and the base 2 is put into the tank body 1 . Add the sample to be analyzed into the gel, and at the same time add the buffer solution into the buffer pool formed by the tank body 1 and the base 2, and then turn on the power to perform the electrophoresis experiment.

Claims (7)

1. vertical electrophoresis system, comprise cell body (1) and be positioned at the pedestal (2) of cell body (1), one side of pedestal (2) is provided with glue chamber (3), glue chamber (3) is communicated with cell body (1) and pedestal (2), the upper edge clamps comb (4) between the glue chamber (3), the wire electrode (5) that is provided with in pedestal (2) and the cell body (1) links to each other with negative pole with positive source respectively, it is characterized in that: described glue chamber (3) is combined by the gel slab that two sections are the U type, above-mentioned two clotting offset plate openings are oppositely arranged, the inside sidewalls that wherein is fixed on the immobilized gel plate (3-1) of pedestal (2) one sides cooperates fastening with the sidewall side dimension of the movable gel slab of another piece (3-2), when movable gel slab (3-2) was installed on the immobilized gel plate (3-1), both lower surfaces were on the same horizontal line, the height of immobilized gel plate (3-1) upper surface is lower than the height of movable gel slab (3-2) upper surface.

2. vertical electrophoresis according to claim 1 system is characterized in that: the guide piece (3-3) that offers longitudinal extension on the sidewall outside of the inside sidewalls of described immobilized gel plate (3-1) and movable gel slab (3-2) ordinatedly.

3. vertical electrophoresis according to claim 2 system, it is characterized in that: described guide piece (3-3) is guide rail and guide groove.

4. vertical electrophoresis according to claim 1 and 2 system, it is characterized in that: described movable gel slab (3-2) is two or more than two, when movable gel slab (3-2) was installed on the immobilized gel plate (3-1), the upper surface height of movable gel slab (3-2) from inside to outside increased gradually.

5. vertical electrophoresis according to claim 1 and 2 system, it is characterized in that: the insulation barrier (3-4) that also includes the sheet more than one or, the thickness of insulation barrier (3-4) is consistent with the thickness of glue chamber (3), and the width of insulation barrier (3-4) is less than the width of glue chamber (3).

6. vertical electrophoresis according to claim 5 system, it is characterized in that: the height of described insulation barrier (3-4) is consistent with the height of fixed dam.

7. vertical electrophoresis according to claim 1 system, it is characterized in that: described movable gel slab (3-2) and/or comb (4) are provided with scale.

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