CN1541983A - Production method of compound microbial fertilizer - Google Patents
Production method of compound microbial fertilizer Download PDFInfo
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- CN1541983A CN1541983A CNA2003101054852A CN200310105485A CN1541983A CN 1541983 A CN1541983 A CN 1541983A CN A2003101054852 A CNA2003101054852 A CN A2003101054852A CN 200310105485 A CN200310105485 A CN 200310105485A CN 1541983 A CN1541983 A CN 1541983A
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- 239000003337 fertilizer Substances 0.000 title claims abstract description 20
- 238000004519 manufacturing process Methods 0.000 title claims description 10
- 230000000813 microbial effect Effects 0.000 title abstract description 11
- 150000001875 compounds Chemical class 0.000 title abstract description 8
- 241000894006 Bacteria Species 0.000 claims abstract description 18
- 239000002131 composite material Substances 0.000 claims abstract description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 5
- 239000001888 Peptone Substances 0.000 claims abstract description 5
- 108010080698 Peptones Proteins 0.000 claims abstract description 5
- 229920002472 Starch Polymers 0.000 claims abstract description 5
- 239000001913 cellulose Substances 0.000 claims abstract description 5
- 229920002678 cellulose Polymers 0.000 claims abstract description 5
- 239000008103 glucose Substances 0.000 claims abstract description 5
- 235000019319 peptone Nutrition 0.000 claims abstract description 5
- 239000008107 starch Substances 0.000 claims abstract description 5
- 235000019698 starch Nutrition 0.000 claims abstract description 5
- 239000001963 growth medium Substances 0.000 claims abstract description 4
- BPQQTUXANYXVAA-UHFFFAOYSA-N Orthosilicate Chemical compound [O-][Si]([O-])([O-])[O-] BPQQTUXANYXVAA-UHFFFAOYSA-N 0.000 claims abstract description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 3
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 5
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 3
- 239000000203 mixture Substances 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims 2
- 230000001488 breeding effect Effects 0.000 claims 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims 1
- 238000012258 culturing Methods 0.000 claims 1
- 238000011534 incubation Methods 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 239000011780 sodium chloride Substances 0.000 claims 1
- 239000002689 soil Substances 0.000 abstract description 14
- 238000000034 method Methods 0.000 abstract description 10
- 235000015097 nutrients Nutrition 0.000 abstract description 10
- 239000002609 medium Substances 0.000 abstract description 6
- 235000015278 beef Nutrition 0.000 abstract description 4
- 230000001737 promoting effect Effects 0.000 abstract description 4
- 239000008223 sterile water Substances 0.000 abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 abstract description 4
- 230000009466 transformation Effects 0.000 abstract description 3
- 238000010521 absorption reaction Methods 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 230000001461 cytolytic effect Effects 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 1
- 241001074968 Halobacteria Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000235070 Saccharomyces Species 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 230000003203 everyday effect Effects 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 239000003621 irrigation water Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- RMAQACBXLXPBSY-UHFFFAOYSA-N silicic acid Chemical compound O[Si](O)(O)O RMAQACBXLXPBSY-UHFFFAOYSA-N 0.000 description 1
- 235000012239 silicon dioxide Nutrition 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000010902 straw Substances 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
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Abstract
本发明涉及一种具有多种功能的复合微生物菌肥，并能采用混合培养工艺生产的方法。复合微生物菌肥由有互惠效应、能互利共栖的自生固氮菌、硅酸盐菌、酵母菌、溶磷细菌、中温纤维素分解菌等组成。加入到特制的培养基中，培养基用牛肉膏、蛋白胨、可溶性淀粉、KNO3、K2HPO4、MgSO4、FeSO4、葡萄糖、纤维素混合后消毒，用无菌水配成营养液，加入复合菌种后37℃+2℃恒温培养120小时。生产出的肥料成品有效菌种总数大于108个/ml。具有促进土壤养分转化，促进植物养分吸收，提高土壤与植株的抗逆性等功能。产品在常温下可以保质半年。
The invention relates to a compound microbial fertilizer with multiple functions, which can be produced by a mixed culture process. The compound microbial fertilizer is composed of self-generated nitrogen-fixing bacteria, silicate bacteria, yeast, phosphorus-dissolving bacteria, and mesophilic cellulose-decomposing bacteria with mutual benefit and mutual benefit. Add it into a special culture medium, the medium is sterilized after mixing beef extract, peptone, soluble starch, KNO 3 , K 2 HPO 4 , MgSO 4 , FeSO 4 , glucose, and cellulose, and make a nutrient solution with sterile water. After adding the composite strains, culture at a constant temperature of 37°C+2°C for 120 hours. The total number of effective strains of the finished fertilizer produced is greater than 10 8 /ml. It has the functions of promoting the transformation of soil nutrients, promoting the absorption of plant nutrients, and improving the stress resistance of soil and plants. The product can be guaranteed for half a year at room temperature.
Description
(一)技术领域(1) Technical field
本发明属于生物菌肥生产技术领域。The invention belongs to the technical field of biological bacterial fertilizer production.
(二)背景技术(2) Background technology
土壤微生物是土壤重要物质组成之一，在土壤养分转化过程中有重要作用。土壤是微生物的大本营，其生物多样性是其功能多样性的前提条件，而菌种的优化组合是其正面功能充分发挥的重要保证。我国早期研制的微生物肥料多数只含一种微生物。其功能单一(固氮菌只固氮，解钾菌只解钾……)，使用条件严格，使用后效果有时不明显。当前国际上流行多菌种复合或联合的微生物肥料，其功能全面，效果明显，应用条件相对比较粗放，深受农民欢迎。但国外对相关技术实行封锁，国内市场上为数不多的产品都是进口的。研制出功能强大，效果明显，农民欢迎的微生物肥料是生产优质无公害农业产品的关键之一。复合生物菌肥的生产一般采用先纯培养，后组合的工艺，生产成本较高，而且常温保存期短。研究筛选出对作物生长发育和土壤生态环境友好、功能互补、且有互惠效应、能互利共栖的一组菌种，并能采用混合培养工艺生产，将对我国的微生物肥料生产有重大意义。Soil microorganisms are one of the important components of soil and play an important role in the transformation of soil nutrients. Soil is the base camp of microorganisms, and its biodiversity is the prerequisite for its functional diversity, while the optimal combination of strains is an important guarantee for its full positive function. Most of the microbial fertilizers developed in my country in the early stage contain only one kind of microorganism. Its function is single (nitrogen-fixing bacteria only fix nitrogen, potassium-decomposing bacteria only decompose potassium...), the use conditions are strict, and the effect after use is sometimes not obvious. At present, multi-strain compound or combined microbial fertilizers are popular in the world. They have comprehensive functions, obvious effects, and relatively extensive application conditions, and are very popular among farmers. However, related technologies are blocked abroad, and the few products on the domestic market are all imported. Developing a microbial fertilizer with powerful functions and obvious effects, which is welcomed by farmers is one of the keys to produce high-quality pollution-free agricultural products. The production of compound biological bacterial fertilizer generally adopts the process of pure culture first and then combination, which has high production cost and short storage period at room temperature. The research screens out a group of strains that are friendly to crop growth and soil ecological environment, have complementary functions, have mutual benefit effects, and can live in mutual benefit, and can be produced by mixed culture technology, which will be of great significance to the production of microbial fertilizers in my country.
(三)发明内容(3) Contents of the invention
本发明提供一种具有多种功能的复合微生物菌肥，并能采用混合培养工艺生产的方法。The invention provides a compound microbial fertilizer with multiple functions, which can be produced by a mixed culture process.
本发明的通过如下技术措施实现：复合微生物菌肥由有互惠效应、能互利共栖的自生固氮菌、硅酸盐菌、酵母菌、溶磷细菌、中温纤维素分解菌等组成。菌种按照常规方法从土壤中筛选与富集，然后加入到特制的培养基中。培养基用牛肉膏、蛋白胨、可溶性淀粉、KNO3、K2HPO4、MgSO4、FeSO4、葡萄糖、纤维素混合后消毒，用无菌水配成营养液，加入复合菌种后37℃+2℃恒温培养120小时。The present invention is realized through the following technical measures: the composite microbial fertilizer is composed of self-generated nitrogen-fixing bacteria, silicate bacteria, yeast, phosphorus-dissolving bacteria, mesophilic cellulolytic bacteria and the like that have a mutual benefit effect and can live in mutual benefit. The bacteria are screened and enriched from the soil according to conventional methods, and then added to the special medium. The culture medium is mixed with beef extract, peptone, soluble starch, KNO 3 , K 2 HPO 4 , MgSO 4 , FeSO 4 , glucose, and cellulose, then sterilized, and nutrient solution is made with sterile water, and the compound bacteria are added at 37°C+ Incubate at a constant temperature of 2°C for 120 hours.
生产出的肥料成品有效菌种总数大于108个/ml。具有促进土壤养分转化，促进植物养分吸收，提高土壤与植株的抗逆性等功能。产品在常温下可以保质半年。The total number of effective strains of the finished fertilizer produced is greater than 10 8 /ml. It has the functions of promoting the transformation of soil nutrients, promoting the absorption of plant nutrients, and improving the stress resistance of soil and plants. The product can be guaranteed for half a year at room temperature.
(四)附图说明(4) Description of drawings
图1：复合微生物菌肥生产方法流程Figure 1: Process flow of the production method of compound microbial fertilizer
图1中1是土壤微生物的常规筛选；2是将筛选后的菌种按照常规方法富集培养；3是定量称取培养基用牛肉膏、蛋白胨、可溶性淀粉、KNO3、K2HPO4、MgSO4、FeSO4、葡萄糖、纤维素；4是将培养基原料粉碎，混匀；5是对培养基消毒。消毒的方法根据生产量的大小选择紫外线法或蒸汽法；6是将消毒后的培养基加入定量的无菌水配制成营养液；7是将流程2富集后的菌液加入到营养液中培养。培养温度为37℃+2℃，恒温培养时间为120小时。培养期间每天需要将培养液振摇和放气。获得的产品为带有酸甜气味的棕色液体。In Figure 1, 1 is the conventional screening of soil microorganisms; 2 is enriching and cultivating the strains after screening according to conventional methods; 3 is quantitatively weighing beef extract, peptone, soluble starch, KNO 3 , K 2 HPO 4 , MgSO 4 , FeSO 4 , glucose, cellulose; 4, pulverize and mix the medium raw materials; 5, sterilize the medium. The method of disinfection is to choose ultraviolet method or steam method according to the size of the production volume; 6 is to add quantitative sterile water to the sterilized medium to prepare a nutrient solution; 7 is to add the enriched bacterial solution in
(五)具体实施方式(5) Specific implementation methods
实施例：用常规土壤微生多筛选方法固氮菌、硅酸、盐菌、酵母菌、溶磷细菌中温纤维素分解菌等(1)，将筛选出的细菌富集培养(2)，然后按以下成份和重量比配制细菌培养基(3)：Embodiment: use conventional soil microbial multi-screening method nitrogen-fixing bacteria, silicic acid, halobacteria, saccharomyces, phosphate-dissolving bacteria, mesophilic cellulolytic bacteria, etc. The following ingredients and weight ratio prepare bacterial culture medium (3):
牛肉膏 5000gBeef Extract 5000g
蛋白胨 10000gPeptone 10000g
可溶性淀粉 20000gSoluble starch 20000g
KNO3 20000g KNO3 20000g
K2HPO4 1000gK 2 HPO 4 1000g
MgSO4 500g MgSO4 500g
FeSO4 10gFeSO 4 10g
Nacl 5000gNacl 5000g
葡萄糖 10000gGlucose 10000g
纤维素 10000gCellulose 10000g
将上述原料混匀后消毒(4)、(5)，再加入无菌水1000000ml配制成营养液(6)，加入富集培养后的混合菌种10000ml后摇动5分钟，37℃+2℃培养120小时(7)。培养期间每天将培养液振摇和放气。获得的产品为带有酸甜气味的棕色液体。Mix the above raw materials and sterilize (4), (5), then add 1,000,000ml of sterile water to make a nutrient solution (6), add 10,000ml of enriched cultured mixed strains, shake for 5 minutes, and culture at 37°C+2°C 120 hours (7). The medium was shaken and aerated daily during the culture period. The product obtained was a brown liquid with a sweet-sour odour.
该肥料经稀释后可直接喷洒于土壤中，或随灌溉水施入土壤。也可用于鸡粪、秸秆等有机物料的发酵。The fertilizer can be directly sprayed on the soil after dilution, or applied to the soil with irrigation water. It can also be used for the fermentation of organic materials such as chicken manure and straw.
Claims (3)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101054852A CN1541983A (en) | 2003-11-07 | 2003-11-07 | Production method of compound microbial fertilizer |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2003101054852A CN1541983A (en) | 2003-11-07 | 2003-11-07 | Production method of compound microbial fertilizer |
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| Publication Number | Publication Date |
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| CN1541983A true CN1541983A (en) | 2004-11-03 |
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| CNA2003101054852A Pending CN1541983A (en) | 2003-11-07 | 2003-11-07 | Production method of compound microbial fertilizer |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102978254A (en) * | 2012-12-26 | 2013-03-20 | 东华大学 | Method for culturing bacterial cellulose through pulsation |
| CN106008087A (en) * | 2016-05-19 | 2016-10-12 | 宁夏泰瑞制药股份有限公司 | Microorganism bacterium fertilizer and preparation method thereof |
-
2003
- 2003-11-07 CN CNA2003101054852A patent/CN1541983A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102978254A (en) * | 2012-12-26 | 2013-03-20 | 东华大学 | Method for culturing bacterial cellulose through pulsation |
| CN102978254B (en) * | 2012-12-26 | 2015-04-08 | 东华大学 | Method for culturing bacterial cellulose through pulsation |
| CN106008087A (en) * | 2016-05-19 | 2016-10-12 | 宁夏泰瑞制药股份有限公司 | Microorganism bacterium fertilizer and preparation method thereof |
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