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CN1483807A - Feed microalgae cultivation device - Google Patents

Feed microalgae cultivation device Download PDF

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Publication number
CN1483807A
CN1483807A CNA031338275A CN03133827A CN1483807A CN 1483807 A CN1483807 A CN 1483807A CN A031338275 A CNA031338275 A CN A031338275A CN 03133827 A CN03133827 A CN 03133827A CN 1483807 A CN1483807 A CN 1483807A
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CN
China
Prior art keywords
algae culture
light source
culture bag
bait
diversing bucket
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CNA031338275A
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Chinese (zh)
Inventor
鹰 刘
刘鹰
杨红生
刘保忠
张涛
王金霞
毛劲松
刘石林
张桂臻
俞志明
冯志华
张福绥
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Institute of Oceanology of CAS
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Institute of Oceanology of CAS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute of Oceanology of CAS filed Critical Institute of Oceanology of CAS
Priority to CNA031338275A priority Critical patent/CN1483807A/en
Publication of CN1483807A publication Critical patent/CN1483807A/en
Pending legal-status Critical Current

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M21/00Bioreactors or fermenters specially adapted for specific uses
    • C12M21/02Photobioreactors
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/14Bags
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/48Holding appliances; Racks; Supports
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/06Nozzles; Sprayers; Spargers; Diffusers
    • C12M29/08Air lift
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M31/00Means for providing, directing, scattering or concentrating light
    • C12M31/10Means for providing, directing, scattering or concentrating light by light emitting elements located inside the reactor, e.g. LED or OLED

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Clinical Laboratory Science (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The culture equipment of bait microalgae mainly is formed from microalgae culture bag, light source, air pipe, guide shell, oxygen-enriching pump and tourmaline, in which the microalgae culture bag is mounted on the base seat, and is positioned in the cylindrical steel wire ring, the bottom portion of the microalgae culture bag is equipped with algae liquor discharge outlet which is communicated with bait tank, and the top of the microalgae culture bag is equipped with water inlet pipe communicated with the cultured algae seed, prepared nutrient liquor and disinfected sea water, its interior is equipped with a guide shell, and the air pipe is positioned in the guide shell. Said equipment can implement all-weather high-density culture of bait microalgae, and its method is simple.

Description

The bait micro-algae culture apparatus
Technical field
The present invention relates to aquaculture technology, specifically a kind of culture apparatus of bait micro-algae is used for cultivating the required main hydrobiont bait-bait micro-algae of aquaculture production etc.
Background technology
Through the artificial screening biological feed of pedestrian worker after cultivating of going forward side by side, be aquaculture of aquatic animal, especially the class high-quality bait produced of seed is being brought into play irreplaceable effect aborning.Illumination, salinity, temperature, potential of hydrogen etc. are the main environment factors that influences algal grown, use the main open cultivation of cement pit of adopting in producing at present, this mode equipment is fairly simple, but algae liquid and air contact area are big, be subjected to the harmful animal biological pollution easily, and be subjected to factor affecting such as artificial, season, weather big, make that throughput is low, poor stability, a little less than the ability to ward off risks, produce be in lowly drop into, in expensive, the state that production efficiency is low.Its technological facilities falls behind product quality and the culture benefit that directly has influence on mariculture industry.
Summary of the invention
For overcome existing bait training method poor stability, culture density is low, disadvantages such as the ability to ward off risks is weak, the invention provides a kind of bait micro-algae culture apparatus, this device is enclosed construction, have good stability, characteristics that the ability to ward off risks is strong, can realize the round-the-clock high-density culture of bait micro-algae.
The present invention solves the scheme that its technical problem adopts: mainly be made up of little algae culture bag, light source, tracheae, diversing bucket, oxygen increasing pump and gas stone, wherein: little algae culture bag is installed on the base, be positioned at the cylindrical steel wire ring, little algae culture bag bottom is established algae liquid relief outlet and is communicated with the bait pond, the top is established water inlet pipe and is communicated through the algae kind after cultivating, the nutritive medium for preparing and sterilization seawater, the inner diversing bucket of installing; Tracheae is arranged in diversing bucket, and a termination is received the gas from oxygen increasing pump, and the other end and gas stone join, and the diversing bucket upper/lower terminal is respectively equipped with pod apertures; Described light source is installed in the diversing bucket and/or the Steel Traveler outside or little algae culture bag top;
Described light source divides built-in, external two kinds, and built-in light source adopts fluorescent light source, and external light source adopts fluorescent light source or reflection sunlight color dysprosium lamp; The described fluorescent light source of built-in light source can be 1~4; The described fluorescent light source of external light source is selected 2~6 groups for use, 2~6 every group; Lateral vertical from 5~10 centimeters of little algae culture bag hangs; It is 200~400 watts that described reflection sunlight color dysprosium lamp is selected power for use, hangs from little algae culture bag top 30~60 centimeters; Described Guan Yiduan and cover plate for sealing are installed, and the other end cover plate and body are by being threaded; Described gas stone is suspended between diversing bucket bottom and the pipe; Vacuumize in the described plexi-glass tubular, diameter is 0.06~0.2 meter; Establish strainer between described oxygen increasing pump and the tracheae; It is 35~50 millimeters needle-based strainer that described strainer can adopt diameter, and blend fiber ester millipore filtration is housed in it, and the filter membrane aperture is 0.30~0.65 micron; Little algae culture bag is cylindrical, and diameter is 0.3~0.7 meter, highly is 1.0 meters~2.0 meters, and thickness is 0.06~0.12 millimeter, and material is a polythene PE; Described diversing bucket is the transparent polyethylene material, and thickness is 0.06~0.10 millimeter, and diameter is 1/2~2/3 of a plastics film culture bag, and the diversing bucket bottom is along 1~4 pod apertures of all jumpers, and top is by 1~4 rectangle square hole of top cutter.
The principle of the invention is: in production application, gas stone aeration zone hydrodynamic stream rises along diversing bucket, pod apertures through diversing bucket top is discharged, current have been realized mixing to greatest extent by the diversing bucket circulation exchange in hole up and down, keep the consistence of culture environment, and can make little algae accept impartial illuminance, when weather cloudy day or night, look the illuminance situation, open inside and outside luminescent lamp and/or reflection-type sunlight color dysprosium lamp to replenish illumination.
The invention has the beneficial effects as follows:
1. culture density is big.Because the present invention is provided with the inside and outside light source that replenishes, and makes algal grown be in optimal light intensity, satisfies the growth and breeding needs to greatest extent, the algae cell density of cultivation is big, and can realize the round-the-clock cultivation of bait micro-algae.
2. good stability, the ability to ward off risks are strong.The diversing bucket of the present invention's design makes the circulation continuously in little algae culture bag of algae liquid, can make little algae accept impartial intensity of illumination, and, make micro algae growth stable by other off-set facility (as diversing bucket), be not subjected to the external environment variable effect, have certain ability to ward off risks.
3. pollution-free.Because the present invention adopts air filter, impurity and bacterium in can filtrated air make bait not contaminated.
4. the present invention adopts the pocket type training method, can effectively utilize the space, reduces the cultivation pool floor space; Be easy to the artificial regulatory envrionment conditions, can realize industrialization production.
The present invention also have simple to operate, (compared with prior art, generally can shorten the formula phase 20~25%) with short production cycle, low cost and other advantages.
Description of drawings
Fig. 1 is a structural representation of the present invention.Wherein:---→ table liquid flow path direction,------→ table air flow line.
Embodiment
The present invention is further described below in conjunction with accompanying drawing and example.
As shown in Figure 1, mainly form by little algae culture bag 1, light source, tracheae 2, diversing bucket 3, oxygen increasing pump 11 and the gas stone 14 of plastic film material, wherein: little algae culture bag 1 is installed on the base 8, be positioned at cylindrical steel wire ring 15 with certain intensity, little algae culture bag 1 bottom is established algae liquid relief outlet 13 and is communicated with the bait pond, the top is established water inlet pipe 12 and is communicated through the algae kind after cultivating, the nutritive medium for preparing and sterilization seawater, the inner diversing bucket 3 of installing; Tracheae 2 is arranged in diversing bucket 3, one terminations and receives gas from oxygen increasing pump 11, and the other end joins with the gas stone 14 that is suspended at diversing bucket 3 bottoms and manage between 5, and diversing bucket 3 upper/lower terminals are respectively equipped with pod apertures 7;
Described light source divides built-in, external two kinds, and built-in light source adopts fluorescent light source 4, is embedded in the diversing bucket 3 by managing 5; External light source adopts fluorescent light source 4 to be installed in Steel Traveler 15 outsides, or reflection sunlight color dysprosium lamp 10, is installed in little algae culture bag 1 top, as the additional illumination at cloudy day or night;
The described fluorescent light source 4 of built-in light source can be 1~4 (present embodiment is 4); The described fluorescent light source 4 of external light source is selected 2~6 groups for use, 2~6 every group (present embodiment is 4 groups, 3 every group); 5~10 centimeters vertical hangings (present embodiment is at 5 centimeters vertical hangings) from little algae culture bag 1; It is 200~400 watts (present embodiment adopts 200 watts) that described reflection sunlight color dysprosium lamp 10 is selected power for use, hangs (present embodiment hangs at 60 centimetres top) from plastics film culture bag 1 top 30~60 centimeters.
Described pipe 5 adopts the machine glass materials, and an end casing is with the seal with elastometic washer of O shape, and by glue the leads that fluorescent light source 4 links to each other is seal-installed on the cover plate, and the other end cover plate and body are by being threaded.
Vacuumize in the described pipe 5, diameter is 0.06~0.2 meter (present embodiment is 0.2 meter).
Establish strainer 9 between described oxygen increasing pump 11 and the tracheae 2, it is the needle-based strainer of 35~50 millimeters (present embodiment is 50 millimeters) that its strainer 9 adopts diameter, in blend fiber ester millipore filtration is housed, the filter membrane aperture is 0.30~0.65 micron (present embodiment is 0.5 micron), is used for the impurity and the bacterium of filtrated air.
Little algae culture bag 1 is cylindrical, and diameter is 0.3~0.7 meter, highly is between 1.0 meters~2.0 meters, and the thickness of little algae culture bag 1 is 0.06~0.12 millimeter (present embodiment 0.3 rice diameter, 2.0 meters high, 0.12 mm thick), and material is a polythene PE.
Described diversing bucket 3 is the transparent polyethylene material, thickness is 0.06~0.10 millimeter, for cylindric, diameter be the plastics film culture bag 1/2~2/3 (present embodiment is 0.06 mm thick, diameter be the plastics film culture bag 1/2), diversing bucket 3 bottoms are along 4 square pod apertures 7 of four rectangles of all jumpers, and top is by four rectangle side's pod apertures 7 of top cutter.
Working process of the present invention is: flow into little algae culture bag 1 through the algae kind after cultivating, the nutritive medium for preparing and sterilization seawater by water inlet pipe 12, the gas that oxygen increasing pump 11 produces filters bacterium and impurity through needle-based strainer 9, tracheae 2 connects needle-based strainer 9 gentle stones 14, air after the filtration rises in little algae culture bag 1, driving algae liquid simultaneously rises, 7 places are spilled over to outside the diversing bucket 3 in diversing bucket 3 pod apertures, algae liquid descends, reenter diversing bucket 3 by diversing bucket 3 lower guide discharge orifices 7, finish full cycle.Luminescent lamp 4 and reflection-type sunlight color dysprosium lamp 10 are mainly used in the additional illumination at cloudy day or night; Dress luminescent lamp 4 is used to improve interior lights illumination in the pipe 5.Plastic film Bag 1 bottom is provided with base 8, and cylindrical steel wire ring 15 is arranged on the base 8, is used for fixing little algae culture bag.Algae liquid after cultivating well flow to the bait pond by aperture 13, supports to grow to produce and uses.

Claims (10)

1. bait micro-algae culture apparatus, it is characterized in that: mainly form by little algae culture bag (1), light source, tracheae (2), diversing bucket (3), oxygen increasing pump (11) and gas stone (14), wherein: little algae culture bag (1) is installed on the base (8), be positioned at cylindrical steel wire ring (15), little algae culture bag (1) bottom is established algae liquid relief outlet (13) and is communicated with the bait pond, the top is established water inlet pipe (12) and is communicated through the algae kind after cultivating, the nutritive medium for preparing and sterilization seawater, the inner diversing bucket (3) of installing; Tracheae (2) is arranged in diversing bucket (3), and a termination is received the gas from oxygen increasing pump (11), and the other end and gas stone (14) join, and diversing bucket (3) upper/lower terminal is respectively equipped with pod apertures (7); Described light source is installed in diversing bucket (3) lining and/or Steel Traveler (15) outside or little algae culture bag (1) top.
2. bait micro-algae culture apparatus according to claim 1 is characterized in that: described light source divides built-in, external two kinds, and built-in light source adopts fluorescent light source (4), and external light source adopts fluorescent light source (4) or reflection sunlight color dysprosium lamp (10).
3. bait micro-algae culture apparatus according to claim 2 is characterized in that: the described fluorescent light source of built-in light source (4) can be 1~4; The described fluorescent light source of external light source (4) is selected 2~6 groups for use, 2~6 every group; Lateral vertical from 5~10 centimeters of little algae culture bag (1) hangs; It is 200~400 watts that described reflection sunlight color dysprosium lamp (10) is selected power for use, hangs from little algae culture bag (1) top 30~60 centimeters.
4. bait micro-algae culture apparatus according to claim 2 is characterized in that: described pipe (5) one ends and cover plate for sealing are installed, and the other end cover plate and body are by being threaded.
5. bait micro-algae culture apparatus according to claim 1 is characterized in that: described gas stone (14) is suspended between diversing bucket (3) bottom and the pipe (5).
6. bait micro-algae culture apparatus according to claim 5 is characterized in that: described plexi-glass tubular vacuumizes in (5), and diameter is 0.06~0.2 meter.
7. bait micro-algae culture apparatus according to claim 1 is characterized in that: establish strainer (9) between described oxygen increasing pump (11) and the tracheae (2).
8. bait micro-algae culture apparatus according to claim 7 is characterized in that: it is 35~50 millimeters needle-based strainer that described strainer (9) can adopt diameter, and blend fiber ester millipore filtration is housed in it, and the filter membrane aperture is 0.30~0.65 micron.
9. bait micro-algae culture apparatus according to claim 1 is characterized in that: little algae culture bag (1) is for cylindrical, and diameter is 0.3~0.7 meter, highly is 1.0 meters~2.0 meters, and thickness is 0.06~0.12 millimeter, and material is a polythene PE.
10. bait micro-algae culture apparatus according to claim 1, it is characterized in that: described diversing bucket (3) is the transparent polyethylene material, thickness is 0.06~0.10 millimeter, diameter is 1/2~2/3 of a plastics film culture bag, diversing bucket (3) bottom is along 1~4 pod apertures of all jumpers (7), and top is by 1~4 rectangle square hole of top cutter.
CNA031338275A 2003-06-27 2003-06-27 Feed microalgae cultivation device Pending CN1483807A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CNA031338275A CN1483807A (en) 2003-06-27 2003-06-27 Feed microalgae cultivation device

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Application Number Priority Date Filing Date Title
CNA031338275A CN1483807A (en) 2003-06-27 2003-06-27 Feed microalgae cultivation device

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CN1483807A true CN1483807A (en) 2004-03-24

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102533522A (en) * 2011-11-30 2012-07-04 镇江绿能环保科技有限公司 Full-plastic airtight modularized airlift light biological reactor
CN102864070A (en) * 2012-10-24 2013-01-09 南京农业大学 Adjustable photobioreactor for high-density culture of microalgae
CN103725590A (en) * 2012-10-12 2014-04-16 台美生物资源股份有限公司 Algae cultivation bag equipment with function of improving illumination efficiency
TWI448241B (en) * 2011-06-10 2014-08-11 Taiam Pan Algasis Inc Culture bag structure
US9464752B2 (en) 2011-06-15 2016-10-11 Lenovo (Singapore) Pte. Ltd. Monitor stand assembly
WO2019121933A1 (en) * 2017-12-22 2019-06-27 Suez Groupe Photo bioreactor
WO2022268732A1 (en) * 2021-06-22 2022-12-29 Pure Algae Denmark Aps A method for growing algae and underwater plants and bioreactors therefor

Cited By (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
TWI448241B (en) * 2011-06-10 2014-08-11 Taiam Pan Algasis Inc Culture bag structure
US9464752B2 (en) 2011-06-15 2016-10-11 Lenovo (Singapore) Pte. Ltd. Monitor stand assembly
CN102533522A (en) * 2011-11-30 2012-07-04 镇江绿能环保科技有限公司 Full-plastic airtight modularized airlift light biological reactor
CN102533522B (en) * 2011-11-30 2013-07-24 镇江绿能环保科技有限公司 Full-plastic airtight modularized airlift light biological reactor
CN103725590A (en) * 2012-10-12 2014-04-16 台美生物资源股份有限公司 Algae cultivation bag equipment with function of improving illumination efficiency
CN102864070A (en) * 2012-10-24 2013-01-09 南京农业大学 Adjustable photobioreactor for high-density culture of microalgae
WO2019121933A1 (en) * 2017-12-22 2019-06-27 Suez Groupe Photo bioreactor
FR3075815A1 (en) * 2017-12-22 2019-06-28 Suez Groupe photobioreactor
CN111511892A (en) * 2017-12-22 2020-08-07 苏伊士集团 photobioreactor
US11639488B2 (en) 2017-12-22 2023-05-02 Suez International Photo bioreactor
WO2022268732A1 (en) * 2021-06-22 2022-12-29 Pure Algae Denmark Aps A method for growing algae and underwater plants and bioreactors therefor

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